A large-range, multi-angle optical imaging system with an ultra-large imaging space

Abstract

The present invention provides a large-range multi-angle optical imaging system with an ultra-large imaging space, which relates to the field of laser scanning microscopic imaging technology, including a main gantry, a mainframe assembly is arranged on the top of the main gantry, an XY translation assembly is arranged between the main gantry and the mainframe assembly, a dual-axis rotating objective stage is arranged at the head of the mainframe assembly, a Z-axis translation assembly is arranged between the mainframe assembly and the objective stage, a signal detection device is arranged behind the dual-axis rotating objective stage, and a two-photon holographic light stimulation assembly can be installed on the top of the mainframe. A three-legged large-space gantry is adopted to provide an infinite imaging space; a large-stroke XY translation assembly and a Z-axis translation assembly realize a large-range three-dimensional movement to meet the imaging requirements of different positions in the imaging space; a two-axis rotating objective stage drives the objective lens to rotate in the horizontal direction and the vertical direction to meet the imaging requirements of different angles; a two-photon holographic light stimulation assembly can also be installed to perform two-photon holographic light stimulation to meet the imaging and two-photon holographic light stimulation requirements.

Description

Technical Field

[0001] The present invention relates to the field of microscopic imaging technology, and in particular to a large-range multi-angle optical imaging system with an ultra-large imaging space. Background Art

[0002] In recent years, the demand for microscopic imaging equipment in the field of scientific research has been continuously undergoing technological breakthroughs and developments. These devices need to meet the imaging needs of different samples, with higher resolution and deeper depth imaging. In order to meet the diverse needs of experiments, it is necessary to design a large-scale multi-angle optical imaging system with a super-large imaging space. Summary of the invention

[0003] Technical issues solved

[0004] In view of the deficiencies of the prior art, the present invention provides a large-range multi-angle optical imaging system with an ultra-large imaging space, which solves the problems existing in the prior art.

[0005] Technical Solution

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a large-range multi-angle optical imaging system with an ultra-large imaging space, comprising a main gantry, a main machine component is provided on the top of the main gantry, an XY translation component is provided between the main gantry and the main machine component, a dual-axis rotating objective stage is provided at the head of the main machine component, a Z-axis translation component is provided between the main machine component and the dual-axis rotating objective stage, the rear of the dual-axis rotating objective stage is connected to a signal collection component, and a laser introduction light path box is arranged below the main gantry.

[0007] Preferably, the main gantry includes a gantry support and a first installation box, a second installation box and a third installation box, and the first installation box, the second installation box and the third installation box are all provided with platform reflectors inside.

[0008] Preferably, the host assembly comprises a host housing, and a host optical element is disposed inside the host housing.

[0009] Preferably, the XY translation assembly includes a mounting platform interconnected with the main gantry, an electrically controlled X mobile platform and an electrically controlled Y mobile platform are arranged above the mounting platform, an optical path arrangement box is arranged on the left side of the electrically controlled X mobile platform, a first platform reflector and a second platform reflector are arranged inside the optical path arrangement box, the first platform reflector is connected to the electrically controlled X mobile platform, the second platform reflector is connected to the electrically controlled Y mobile platform, the first platform reflector is connected to the electrically controlled X mobile platform, and the second platform reflector is connected to the electrically controlled Y mobile platform.

[0010] Preferably, the Z-axis translation assembly is located at the head of the main assembly, and the Z-axis translation assembly includes an objective lens stand, and the objective lens stand is connected to the main assembly via a driving motor.

[0011] Preferably, the dual-axis rotating objective stage comprises a horizontal rotating structure and a vertical rotating structure. The dual-axis rotating objective stage is connected to a signal collecting component. The signal collecting component comprises a signal collecting component housing, a collecting optical path is provided inside the signal collecting component housing, and the signal collecting component housing is connected to a photomultiplier tube.

[0012] Preferably, a two-photon holographic light stimulation component may also be installed on the top surface of the host component.

[0013] Preferably, the two-photon holographic light stimulation component includes a two-photon holographic light stimulation housing and a two-photon holographic light stimulation host bracket interconnected with the host component, and two-photon holographic light stimulation optical elements are provided on both sides of the two-photon holographic light stimulation bracket.

[0014] Preferably, the bottom of the main gantry is fixed to the optical platform by bolting, and the main gantry and the XY translation assembly are bolted to each other, and the XY translation assembly and the host assembly are positioned to each other by a dovetail groove.

[0015] Preferably, the laser is optically connected with the laser introduction optical element in the laser introduction optical path box, the laser introduction optical element is optically connected with the first installation box body in the main gantry, the first installation box body, the second installation box body and the third installation box body in the main gantry are optically connected, the third installation box body in the main gantry is optically connected with the platform reflector in the optical path arrangement box body of the XY translation assembly, and the platform reflector in the optical path arrangement box body of the XY translation assembly and the host optical element of the host assembly are optically connected with each other.

[0016] Preferably, the optical path of the two-photon holographic optical stimulation component and the host optical element of the host component are optically connected to each other.

[0017] Beneficial Effects

[0018] The present invention provides a large-range multi-angle optical imaging system with an ultra-large imaging space. It has the following beneficial effects:

[0019] The system of the present invention adopts a three-legged large-space gantry to provide an infinite imaging space, allowing various types of imaging objects to be accommodated.

[0020] The system of the present invention uses a large-stroke XY translation component to drive the entire microscope to move over a large range in the XY direction, meeting the needs of imaging at different positions in a large imaging space.

[0021] The system of the present invention adopts a high-precision Z-axis translation component, which moves the object stage in the Z-axis direction to meet the imaging requirements of different depths.

[0022] The system of the present invention adopts a dual-axis rotating objective stage, which allows the objective lens to rotate horizontally and vertically to meet the needs of multi-angle imaging.

[0023] In this system, a two-photon holographic light stimulation component can be installed above the host component to meet the needs of two-photon holographic light stimulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is the overall structure diagram of the present invention;

[0025] Figure 2 It is the main gantry structure diagram of the present invention;

[0026] Figure 3 It is a structural diagram of the host component of the present invention;

[0027] Figure 4 It is a structural diagram of the XY translation stage of the present invention;

[0028] Figure 5 It is a structural diagram of the Z translation stage of the present invention;

[0029] Figure 6 It is a structural diagram of a two-axis rotating objective lens stage of the present invention;

[0030] Figure 7 It is a structural diagram of the two-photon holographic light stimulation component of the present invention;

[0031] Legend: 1. Main gantry; 101. Gantry support; 102. First installation box; 103. Second installation box; 104. Third installation box; 2. Host assembly; 201. Host housing; 202. Host optical element; 3. XY translation assembly; 301. Installation platform; 302. Electric-controlled X-moving platform; 303. Electric-controlled Y-moving platform; 304. Light path arrangement box; 305. First platform reflector; 306. Second platform reflector; 4. Z-axis translation assembly; 401, objective lens stand; 402, drive motor; 5, dual-axis rotating objective lens stage; 501, horizontal rotating structure; 502, vertical rotating structure; 6, two-photon holographic light stimulation assembly; 601, two-photon holographic light stimulation housing; 602, two-photon holographic light stimulation host bracket; 603, two-photon holographic light stimulation optical element; 7, signal collection assembly; 701, signal collection assembly housing; 702, photomultiplier tube; 8, laser introduction optical path box. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Specific embodiment one:

[0034] As shown in the figure, a large-range multi-angle optical imaging system with an ultra-large imaging space includes a main gantry 1, a main machine component 2 is provided on the top of the main gantry 1, an XY translation component 3 is provided between the main gantry 1 and the main machine component 2, a dual-axis rotating objective lens stage 5 is provided at the head of the main machine component 2, a Z-axis translation component 4 is provided between the main machine component 2 and the dual-axis rotating objective lens stage 5, a signal detection device is provided behind the dual-axis rotating objective lens stage, and a two-photon holographic light stimulation component 6 can be installed on the top of the main machine component 2.

[0035] In this embodiment, the main gantry 1 adopts a three-legged large-space gantry. The gantry is composed of three vertical support frames and two horizontal load-bearing beams, which are connected to each other by bolts. The bottom of the three support frames is equipped with a mounting seat to fix the entire system. A flat-type plane load-bearing platform is installed on the load-bearing beam, and the host component 2 is installed on the load-bearing platform to reduce stress and increase the load-bearing capacity of the gantry. In this embodiment, the main gantry 1 provides a space of 800mmWx800mmDx360mmH to accommodate imaging targets, allowing the placement of animal behavior equipment such as mice running balls or accommodating large model animals such as marmosets. In this embodiment, the main gantry 1 is hollow inside and is provided with a reflector mounting box, allowing access to the optical path and construction of the optical path inside the gantry.

[0036] In this embodiment, the long-stroke XY translation assembly 3 is bolted to the main gantry 1 through the mounting platform 301, and the electrically controlled XY mobile platform above the mounting platform 301 drives the main assembly 2 to move in a large range in the XY direction. In this embodiment, the XY direction movement range reaches 140mm, and the control accuracy reaches 1μm, allowing a large range of visual fields to be searched without moving the sample, and the target imaging area is located. The long-stroke XY translation assembly 3 is provided with an optical path arrangement box 304, and the optical path arrangement box 304 guides the incident light from the main gantry 1 into the main assembly. The interior of the optical path arrangement box 304 is provided with a first platform reflector 305 and a second platform reflector 306. The first platform reflector 305 is bolted to the electrically controlled X mobile platform 302 and moves therewith, and remains parallel to the two-dimensional translation stage in the X and Y directions. The second platform reflector 306 is bolted to the electrically controlled Y mobile platform 303 and moves therewith, and remains parallel to the two-dimensional translation stage in the Y direction. The first platform reflector 305 and the second platform reflector 306 remain parallel in the Y direction and relatively stationary in the X direction. The incident light from the main gantry 1 is incident along the X direction of the two-dimensional translation stage, turns to the Y direction through the first platform reflector 305, and turns to enter the main unit through the second platform reflector 306. When the two-dimensional translation stage moves along the X direction, since the first platform reflector 305 is parallel to the two-dimensional translation stage in the X direction, the position and angle of the light incident on the first platform reflector 305 will not change with the displacement of the translation stage. According to the law of reflection, the position and angle of the reflected light relative to the first platform reflector 305 will not change either, and the first platform reflector 305 and the second platform reflector 306 are relatively stationary in the X direction, so the overall optical path of the system will not change due to the movement of the two-dimensional translation stage in the X direction; the light reflected by the first platform reflector 305 is incident on the second platform reflector 306 along the Y direction. When the two-dimensional translation stage moves along the Y direction, the second platform reflector 306 moves with the Y-direction motion module of the two-dimensional translation stage. Since the first platform reflector 305 and the second platform reflector 306 remain parallel in the Y direction, the position and angle of the light incident on the second platform reflector 306 will not change. According to the law of reflection, the position and direction of the reflected light relative to the second platform reflector 306 will not change either, so the overall optical path of the system will not change due to the movement of the two-dimensional translation stage in the Y direction.

[0037] In this embodiment, the Z-axis translation assembly 4 is located at the head of the main assembly 2, and includes an objective lens stage 401 and a dual drive motor 402. One side of the drive motor 402 is bolted to the main assembly 2, and the other side is bolted to the objective lens stage 401. The drive motor 402 drives the objective lens stage 401 to translate in the Z direction. The dual drive motors are used to ensure high-precision and high-stability translation in the Z direction. The total stroke can reach 25 mm, and the control accuracy can reach 0.1 μm, which can meet the imaging requirements of different depths.

[0038] In this embodiment, the two-axis rotating objective lens stage 5 includes a horizontal rotating structure 501 and a vertical rotating structure 502. Both the horizontal rotating structure and the vertical rotating structure adopt a boss type rotating pair structure. In the boss type rotating pair structure, the boss structures 501-1 and 502-1 are connected to the pressing plates 501-2 and 502-2 through threads to constrain the axial degree of freedom. The horizontal rotating structure allows the objective lens to rotate horizontally.

[0039] The vertical rotation structure allows the objective lens to rotate ±90° in the vertical direction, and the vertical rotation structure allows the objective lens to rotate ±120° in the vertical direction to meet the imaging requirements at different angles;

[0040] In this embodiment, a two-photon holographic light stimulation component 6 can be installed above the host component 2, and the two-photon holographic light stimulation component 6 includes a two-photon holographic light stimulation housing 601, a two-photon holographic light stimulation host bracket 602, and a two-photon holographic light stimulation optical element 603. The two-photon holographic light stimulation host bracket is bolted to the host component 2, and two-photon holographic light stimulation optical elements 603 are installed on both sides of the two-photon holographic light stimulation host bracket 602. The two-photon holographic light stimulation component 6 is interconnected with the host component 2 and the host optical element 202 of the host component 2. Specific embodiment 2:

[0042] A large-range multi-angle optical imaging system with an ultra-large imaging space comprises a main gantry 1, a mainframe component 2 is arranged on the top of the main gantry 1, an XY translation component 3 is arranged between the main gantry 1 and the mainframe component 2, a double-axis rotating objective lens stage 5 is arranged at the head of the mainframe component 2, a Z-axis translation component 4 is arranged between the mainframe component 2 and the double-axis rotating objective lens stage 5, the rear of the double-axis rotating objective lens stage 5 is connected to a signal collection component 7, a laser introduction optical path box 8 is arranged below the main gantry 1, the main gantry 1 comprises a gantry support 101 and a first installation box body 102, a second installation box body 103 and a third installation box body 104, and the first installation box body (102), the second installation box body (103) and the third installation box body (104) are provided with The mainframe assembly 2 includes a mainframe housing 201, a mainframe optical element 202 is disposed inside the mainframe housing 201, an XY translation assembly 3 includes a mounting platform 301 interconnected with the main gantry 1, an electrically controlled X-moving platform 302 and an electrically controlled Y-moving platform 303 are disposed above the mounting platform 301, an optical path arrangement box 304 is disposed on the left side of the electrically controlled X-moving platform 302, and a platform reflector is disposed inside the optical path arrangement box 304, a first platform reflector 305 is connected to the electrically controlled X-moving platform 302, a second platform reflector 306 is connected to the electrically controlled Y-moving platform 303, a Z-axis translation assembly 4 is located at the head of the mainframe assembly 2, and the Z-axis translation assembly 4 includes an objective lens stand 401, and the objective lens stand 401 is connected to the mainframe assembly 2 is connected by a driving motor 402, the dual-axis rotating objective lens stage 5 includes a horizontal rotating structure 501 and a vertical rotating structure 502, the dual-axis rotating objective lens stage 5 is connected to the signal collecting component 7, the signal collecting component 7 includes a signal collecting component housing 701, the signal collecting component housing 701 has a collecting light path inside, the signal collecting component housing 701 is connected to the photomultiplier tube 702, the top surface of the host component 2 can also be installed with a two-photon holographic light stimulation component 6, the two-photon holographic light stimulation component 6 includes a two-photon holographic light stimulation housing 601, and a two-photon holographic light stimulation host bracket 602 interconnected with the host component 2, two-photon holographic light stimulation optical elements 603 are arranged on both sides of the bracket, and the bottom of the main gantry 1 is connected to the optical platform by bolts The main gantry 1 and the XY translation assembly 3 are connected and fixed, and the main gantry 1 and the XY translation assembly 3 are bolted to each other, and the XY translation assembly 3 and the main assembly 2 are positioned with each other through a dovetail groove, the laser is optically connected to the laser introduction optical element in the laser introduction optical path box 8, the laser introduction optical element is optically connected to the first installation box body 102 in the main gantry 1, the first installation box body 102, the second installation box body 103, and the third installation box body 104 in the main gantry 1 are optically connected, the third installation box body 104 in the main gantry 1 is optically connected to the platform reflector in the optical path arrangement box body 304 of the XY translation assembly 3, the platform reflector in the optical path arrangement box body 304 of the XY translation assembly 3 and the main optical element 202 of the main assembly 2 are optically connected to each other,The optical element 603 of the two-photon holographic light stimulation component 6 and the host optical element 202 of the host component 2 are optically connected to each other.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "comprising a reference structure" do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A large-range multi-angle optical imaging system with an ultra-large imaging space, characterized in that: The invention comprises a main gantry (1), a mainframe assembly (2) is arranged on the top of the main gantry (1), an XY translation assembly (3) is arranged between the main gantry (1) and the mainframe assembly (2), a double-axis rotating objective lens stage (5) is arranged at the head of the mainframe assembly (2), a Z-axis translation assembly (4) is arranged between the mainframe assembly (2) and the double-axis rotating objective lens stage (5), the rear of the double-axis rotating objective lens stage (5) is connected to a signal collection assembly (7), a laser introduction optical path box (8) is arranged below the main gantry (1), the XY translation assembly (3) comprises a mounting platform (301) interconnected with the main gantry (1), and the upper portion of the mounting platform (301) is provided with a plurality of optical path boxes (8) arranged below the main gantry (1), and the upper portion of the mounting platform (301) is provided with a plurality of optical path boxes (8) arranged below the main gantry (1). An electrically controlled X-moving platform (302) and an electrically controlled Y-moving platform (303) are provided, an optical path arrangement box (304) is provided on the left side of the electrically controlled X-moving platform (302), the first installation box (102), the second installation box (103) and the third installation box (104) in the main gantry (1) are optically connected, the third installation box (104) in the main gantry (1) is optically connected to the platform reflector in the optical path arrangement box (304) of the XY translation assembly (3), and the platform reflector in the optical path arrangement box (304) of the XY translation assembly (3) is optically connected to the host optical element (202) of the host assembly (2).

2. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: The main gantry (1) comprises a gantry support (101) and a first installation box (102), a second installation box (103) and a third installation box (104), and platform reflectors are arranged inside the first installation box (102), the second installation box (103) and the third installation box (104).

3. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: The host assembly (2) comprises a host housing (201), and a host optical element (202) is arranged inside the host housing (201).

4. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: A first platform reflector (305) and a second platform reflector (306) are arranged inside the optical path arrangement box (304); the first platform reflector (305) is connected to the electrically controlled X movable platform (302); and the second platform reflector (306) is connected to the electrically controlled Y movable platform (303).

5. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: The Z-axis translation assembly (4) is located at the head of the mainframe assembly (2), and the Z-axis translation assembly (4) comprises an objective lens stand (401), and the objective lens stand (401) is connected to the mainframe assembly (2) via a driving motor (402).

6. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: The dual-axis rotating objective stage (5) comprises a horizontal rotating structure (501) and a vertical rotating structure (502); the dual-axis rotating objective stage (5) is connected to a signal collecting component (7); the signal collecting component (7) comprises a signal collecting component housing (701); a collecting light path is provided inside the signal collecting component housing (701); and the signal collecting component housing (701) is connected to a photomultiplier tube (702).

7. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: A two-photon holographic light stimulation component (6) may also be installed on the top surface of the host component (2).

8. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 7, characterized in that: The two-photon holographic light stimulation component (6) comprises a two-photon holographic light stimulation housing (601) and a two-photon holographic light stimulation host bracket (602) interconnected with the host component (2), and two-photon holographic light stimulation optical elements (603) are arranged on both sides of the two-photon holographic light stimulation bracket.

9. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 1, characterized in that: The bottom of the main gantry (1) is fixed to the optical platform by bolting, and the main gantry (1) and the XY translation assembly (3) are bolted to each other, and the XY translation assembly (3) and the main machine assembly (2) are positioned to each other by dovetail grooves.

10. The large-range multi-angle optical imaging system with a super-large imaging space according to claim 7, characterized in that: The optical path of the two-photon holographic light stimulation optical element (603) and the host optical element (202) of the host component (2) are optically connected to each other.

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