Anti-aging whitening moisturizing composition containing caviar extract and application thereof

Abstract

The present application relates to a kind of anti-aging whitening moisturizing composition containing caviar extract and its application, the anti-aging whitening moisturizing composition includes sturgeon roe extract 1-10 parts, salmon roe extract 0.5-5 parts, N-acetylneuraminic acid 5-10 parts and tranexamic acid 0.5-3 parts by mass fraction.The present application creatively sturgeon roe extract, salmon roe extract, N-acetylneuraminic acid and tranexamic acid four active ingredients are compounded to form a new composition, the composition has excellent anti-aging, whitening, moisturizing effect, can be applied to the preparation of various cosmetics, mild and non-irritating.Four components have potential interaction between each other, mutually positive influence.When four components are combined above the specific mass ratio, the efficacy of anti-aging, whitening, moisturizing can be maximized.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and relates to an anti-aging whitening moisturizing composition containing caviar extract and application thereof, in particular to an anti-aging whitening moisturizing composition containing caviar extract and application thereof in preparation of cosmetics. BACKGROUND

[0002] Skin is continuously exposed to the outside world, including environmental pollution, ultraviolet radiation and other factors, which continuously produce free radicals, causing the skin to gradually age. Free radicals are the byproducts of human cell metabolism, and excessive free radicals have destructive effects on unsaturated fatty acids, protein molecules, nucleic acid molecules, extracellular soluble components and membrane lipids, causing wrinkles, sagging and subcutaneous fat reduction. Free radicals destroy collagen and elastin, causing the skin to lose its moisturizing and elasticity maintaining functions, and accelerate the deposition of melanin. Age and certain external factors can cause the body and skin tissue to produce more free radicals than the body's normal ability to remove free radicals, thereby damaging the skin tissue and leading to aging.

[0003] At the same time, when the environment changes, the stratum corneum of the skin cannot adjust enough moisturizing factors in time, and the activity of sebaceous glands will also decrease, causing the oil and water on the face to decrease, and the skin to appear tight and even have fine lines around the eyes. Therefore, when taking care of the skin in daily life, it is necessary to maintain the moisture of the skin, reduce the loss of moisture of the skin, and thus maintain a certain water-oil balance state of the skin.

[0004] Moisturizing and antioxidant are important links to maintain young skin and delay aging, and whitening is also one of the main demands of many consumers. The depth of skin color is controlled by many factors and mainly depends on the content and distribution of melanin. Melanin is an amino acid derivative formed by a series of chemical reactions of tyrosine or 3,4-dihydroxyphenylalanine, which often exists in a polymerized form. Melanin is synthesized by melanocytes in the basal layer of human epidermis, and when ultraviolet rays are irradiated onto the skin, the skin will be in a "self-protection" state, activating the activity of tyrosinase to catalyze the oxidation of tyrosine or 3,4-dihydroxyphenylalanine to generate melanin to protect our skin cells. Melanin moves through layers of cell metabolism to the epidermis of the skin to form freckles, sunspots and dark spots. Therefore, it is very meaningful to develop a cosmetic product that effectively moisturizes, resists oxidation and whitens. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application aims to provide an anti-aging whitening moisturizing composition containing caviar extract and application thereof, in particular to provide an anti-aging whitening moisturizing composition containing caviar extract and application thereof in preparation of cosmetics.

[0006] To achieve the object of the present application, the present application adopts the following technical solutions:

[0007] In a first aspect, the present application provides an anti-aging, whitening and moisturizing composition containing caviar extract, which comprises 1-10 parts of sturgeon caviar extract, 0.5-5 parts of salmon caviar extract, 5-10 parts of N-acetylneuraminic acid and 0.5-3 parts of tranexamic acid.

[0008] The mass fraction of the sturgeon caviar extract can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the salmon caviar extract can be 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.; the mass fraction of the N-acetylneuraminic acid can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; and the mass fraction of the tranexamic acid can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc. Other specific point values within the above numerical ranges can also be selected, which will not be described here one by one.

[0009] The present application creatively combines sturgeon caviar extract, salmon caviar extract, N-acetylneuraminic acid and tranexamic acid to form a new composition, which has excellent anti-aging, whitening and moisturizing effects and can be applied to the preparation of various cosmetics without irritation. The four components have potential interactions and mutual positive effects. When the four components are combined in the above specific mass ratio, the anti-aging, whitening and moisturizing effects can be maximized.

[0010] The present application also creatively develops a more preferred preparation method of sturgeon caviar extract, which makes the anti-aging, whitening and moisturizing effects of the composition more significant. Specifically as follows:

[0011] Preferably, the sturgeon caviar extract is prepared by a preparation method comprising the following steps:

[0012] (S1) After the sturgeon caviar is crushed, it is mixed with protease for enzymatic hydrolysis treatment, the enzymatic hydrolysate is centrifuged, and the supernatant is taken;

[0013] (S2) The supernatant is mixed with phosphoric acid for degumming treatment, centrifuged, and the supernatant is taken;

[0014] (S3) The supernatant is mixed with alkali for deacidification treatment, centrifuged, and the supernatant is taken;

[0015] (S4) The supernatant is subjected to decolorization and deodorization treatment to obtain sturgeon caviar extract.

[0016] Preferably, the protease in step (S1) is a combination of papain and subtilisin.

[0017] The caviar extract is obtained by complex enzyme protease and subtilisin protease enzymolysis, so that the product is more easily absorbed by the skin and the efficacy is promoted; meanwhile, the excellent enzymolysis product distribution mode can also better improve the comprehensive effect of the product.

[0018] Preferably, the added amount of the protease is 3000-5000 U / g, such as 3000 U / g, 3500 U / g, 4000 U / g, 4500 U / g, 5000 U / g, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0019] Preferably, the enzymolysis treatment in step (S1) is carried out at pH = 6-8 (such as pH = 6, pH = 7, pH = 8, etc.), temperature 36-45℃ (such as 36℃, 38℃, 40℃, 42℃, 45℃, etc.) for 3-8h (such as 3h, 4h, 5h, 6h, 7h, 8h, etc.). Other specific point values in the above numerical range can be selected, which will not be repeated here.

[0020] Preferably, the added amount of the phosphoric acid in step (S2) is 1.0-2.0% of the supernatant, such as 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, etc.

[0021] Preferably, the degumming treatment is carried out at 60-70℃ (such as 60℃, 62℃, 64℃, 66℃, 70℃, etc.) for 0.5-2h (such as 0.5h, 0.8h, 1h, 1.2h, 1.5h, 2h, etc.).

[0022] Preferably, the added amount of the alkali in step (S3) is 1.0-2.0% of the supernatant, such as 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, etc.

[0023] Preferably, the deacidification treatment is carried out at 50-60℃ (such as 50℃, 52℃, 54℃, 56℃, 60℃, etc.) for 0.5-2h (such as 0.5h, 0.8h, 1h, 1.2h, 1.5h, 2h, etc.).

[0024] Preferably, the centrifugation is independently carried out at 3000-5000 rpm (such as 3000 rpm, 3500 rpm, 4000 rpm, 4500 rpm, 5000 rpm, etc.) for 5-15 min (such as 5 min, 8 min, 9 min, 10 min, 12 min, 13 min, 14 min, 15 min, etc.).

[0025] Other specific point values in the above numerical range can be selected, which will not be repeated here.

[0026] The application also creatively develops a more preferred preparation method of salmon roe extract, so that the composition has more significant effects on anti-aging, whitening and moisturizing. Specifically as follows:

[0027] Preferably, the salmon roe extract is prepared by a preparation method comprising the following steps:

[0028] (T1) After the salmon roe is minced, it is mixed with protease for enzymatic treatment, the enzymatic solution is centrifuged, and the supernatant is taken;

[0029] (T2) The supernatant is mixed with phosphoric acid for degumming treatment, centrifuged, and the supernatant is taken;

[0030] (T3) The supernatant is mixed with alkali for deacidification treatment, centrifuged, and the supernatant is taken;

[0031] (T4) The supernatant is subjected to decolorization and deodorization treatment to obtain the salmon roe extract.

[0032] Preferably, the protease in step (T1) is a combination of papain and neutral protease.

[0033] The salmon roe extract is obtained by enzymatic hydrolysis of a complex enzyme of papain and neutral protease, so that the product is more easily absorbed and utilized by the skin, and the promotion of the effect is facilitated; at the same time, the excellent enzymatic product distribution mode can also better improve the comprehensive effect of the product.

[0034] Preferably, the addition amount of the protease is 1000-3000 U / g, such as 1000 U / g, 1500 U / g, 2000 U / g, 2500 U / g, 3000 U / g, etc. Other specific point values within the above numerical range can also be selected, which will not be repeated here.

[0035] Preferably, the enzymatic treatment in step (T1) is carried out at pH = 6-8 (such as pH = 6, pH = 7, pH = 8, etc.), temperature 35-40℃ (such as 35℃, 36℃, 37℃, 38℃, 40℃, etc.) for 2-5h (such as 2h, 3h, 4h, 5h, etc.). Other specific point values within the above numerical range can also be selected, which will not be repeated here.

[0036] Preferably, the addition amount of phosphoric acid in step (T2) is 0.5-1.0% of the supernatant, such as 0.5%, 0.7%, 0.8%, 1.0%, etc.

[0037] Preferably, the degumming treatment is carried out at 60-70℃ for 0.5-2h (such as 60℃, 62℃, 64℃, 66℃, 70℃, etc.) for 0.5-2h (such as 0.5h, 0.8h, 1h, 1.2h, 1.5h, 2h, etc.).

[0038] Preferably, the amount of the base added in step (T3) is 0.5-1.0% of the supernatant, such as 0.5%, 0.7%, 0.8%, 1.0%, etc.

[0039] Preferably, the deacidification treatment is performed at 50-60℃ for 0.5-2h (such as 50℃, 52℃, 54℃, 56℃, 60℃, etc.) for 0.5-2h (such as 0.5h, 0.8h, 1h, 1.2h, 1.5h, 2h, etc.).

[0040] Preferably, the centrifugation is performed at 3000-5000rpm (such as 3000rpm, 3500rpm, 4000rpm, 4500rpm, 5000rpm, etc.) for 5-15min (such as 5min, 8min, 9min, 10min, 12min, 13min, 14min, 15min, etc.), each independently.

[0041] Other specific point values within the above-mentioned numerical ranges can also be selected, which will not be repeated here.

[0042] In a second aspect, the present application provides use of the caviar extract-containing anti-aging, whitening and moisturizing composition according to the first aspect in the preparation of a cosmetic product.

[0043] Preferably, the cosmetic product includes emulsion, cosmetic water, face cream, face mask, makeup remover, facial cleanser, etc.

[0044] Preferably, the content of the anti-aging, whitening and moisturizing composition in the cosmetic product is 1-10%, such as 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0045] Compared with the prior art, the present application has the following beneficial effects:

[0046] The present application creatively combines four active ingredients, i.e., sturgeon caviar extract, salmon caviar extract, N-acetylneuraminic acid and tranexamic acid, to form a brand-new composition, which has excellent anti-aging, whitening and moisturizing effects and can be applied in the preparation of various cosmetic products without irritation. Moreover, the four components have potential interactions and mutual positive influences. When the four components are combined in the above-mentioned specific mass ratio, the anti-aging, whitening and moisturizing effects can be maximized.

[0047] The present application also creatively develops a more preferred preparation method of sturgeon caviar extract or salmon caviar extract, so that the anti-aging, whitening and moisturizing effects of the above-mentioned composition are more significant. DETAILED DESCRIPTION

[0048] For further illustrating the technical means adopted by the present application and its effects, the technical solutions of the present application will be further explained in combination with the preferred embodiments of the present application, but the present application is not limited in the scope of the embodiments.

[0049] The source information of the raw materials involved in the following preparation examples, examples and comparative examples is as follows:

[0050] The neutral protease, subtilisin and papain are all from the product with food grade of model purchased from Jinyang Bioengineering Co., Ltd.

[0051] The coco-glucoside is from the product with trade name of CoCoGlucoside (C8-14 Fatty Alcohol Glycoside, SpecSufc TM APG0814) purchased from Spec-Chem Industry Inc. Co.

[0052] The polyglyceryl-3 methylglucose distearate is from the product with trade name of Care 450MB purchased from Evonik-Personal Care Co.

[0053] The jojoba seed oil is from the product with trade name of Jojoba Oil purchased from OLVEA Co.

[0054] The shea butter is from the product with trade name of shea butter purchased from Shaanxi Saien Biological Technology Co., Ltd.

[0055] Preparation Example 1-1

[0056] The present preparation example provides a sturgeon roe extract, and the preparation method is as follows:

[0057] (1) The sturgeon roe is washed and dried, then is minced, the pH is adjusted to 7.5, and is mixed with papain and subtilisin with enzyme activity ratio of 1:1 (total enzyme activity 4000 U / g), and is subjected to enzymatic reaction at 40℃ for 4h, the enzymatic solution is centrifuged at 5000 rpm for 10 min, and the supernatant is taken;

[0058] (2) The supernatant is mixed with 1% phosphoric acid solution with concentration of 70%, and is subjected to degumming treatment at 65℃ for 1h, is centrifuged at 5000 rpm for 10 min, and the supernatant is taken;

[0059] (3) The supernatant is mixed with 2% sodium hydroxide solution with concentration of 30%, and is subjected to deacidification treatment at 55℃ for 1h, is centrifuged at 5000 rpm for 10 min, and the supernatant is taken;

[0060] (4) The supernatant is subjected to decolorization and deodorization treatment, and the sturgeon roe extract is obtained.

[0061] Preparation Example 1-2

[0062] This preparation example provides a sturgeon roe extract, the difference between the preparation method and Preparation Example 1-1 is only in step (1) mixed with a single papain (total enzyme activity 4000 U / g), other preparation conditions remain unchanged.

[0063] Preparation Example 1-3

[0064] This preparation example provides a sturgeon roe extract, the difference between the preparation method and Preparation Example 1-1 is only in step (1) mixed with a single subtilisin (total enzyme activity 4000 U / g), other preparation conditions remain unchanged.

[0065] Preparation Example 2-1

[0066] This preparation example provides a salmon roe extract, the preparation method is as follows:

[0067] (1) The salmon roe is washed and dried, then minced, the pH is adjusted to 6.5, mixed with papain and neutral protease with an enzyme activity ratio of 1:1 (total enzyme activity 3000 U / g), and the enzymolysis reaction is carried out at 35°C for 3h, the enzymolysis solution is centrifuged at 4000 rpm for 10 min, and the supernatant is taken;

[0068] (2) The supernatant is mixed with a 70% concentration of 1% phosphoric acid solution, and the degumming treatment is carried out at 65°C for 0.5h, and the supernatant is taken after centrifugation at 4000 rpm for 10 min;

[0069] (3) The supernatant is mixed with a 30% concentration of 2% sodium hydroxide solution, and the deacidification treatment is carried out at 55°C for 0.5h, and the supernatant is taken after centrifugation at 4000 rpm for 10 min;

[0070] (4) The supernatant is subjected to decolorization and deodorization treatment to obtain a salmon roe extract.

[0071] Preparation Example 2-2

[0072] This preparation example provides a salmon roe extract, the difference between the preparation method and Preparation Example 2-1 is only in step (1) mixed with a single papain (total enzyme activity 3000 U / g), other preparation conditions remain unchanged.

[0073] Preparation Example 2-3

[0074] This preparation example provides a salmon roe extract, the difference between the preparation method and Preparation Example 2-1 is only in step (1) mixed with a single neutral protease (total enzyme activity 3000 U / g), other preparation conditions remain unchanged.

[0075] Example 1

[0076] The present example provides a cosmetic containing caviar extract, the formula table of which is as follows:

[0077]

[0078]

[0079] The preparation method is as follows:

[0080] (1) Add water, polyhydric alcohol, and chelating agent into the water phase pot, and heat and stir at 80°C until uniform; add emulsifier, emollient, and thickening agent into the oil phase pot, and heat and stir at 70°C until uniform;

[0081] (2) Draw the mixture in the water pot and oil pot into the emulsification pot, add antioxidant, homogenize at 70°C and 5000 rpm, then cool to 30°C, add skin conditioner and pH adjuster, and stir to obtain the product.

[0082] Example 2

[0083] The present example provides a cosmetic containing caviar extract, the formula table of which is as follows:

[0084] Example 3

[0085] The present example provides a cosmetic containing caviar extract, the formula table of which is as follows:

[0086] Example 4

[0087] The present example provides a cosmetic containing caviar extract, the formula table of which is as follows:

[0088] Example 5

[0089] The present example provides a cosmetic containing caviar extract, the formula table of which is as follows:

[0090] Comparative Example 1

[0091] The comparative example 1 provides a cosmetic containing caviar extract, whose formulation table is as follows:

[0092]

[0093] The preparation method is as follows:

[0094] (1) Water, polyol and chelating agent are added into the water phase pot, and heated and stirred uniformly at 80℃; emulsifier, emollient and thickening agent are added into the oil phase pot, and heated and stirred uniformly at 70℃;

[0095] (2) The mixture in the water pot and the oil pot is pumped into the emulsification pot, antioxidant is added, and after homogenization at 5000 rpm and 70℃, the temperature is lowered to 30℃, pH regulator is added, and stirred to obtain the product.

[0096] Comparative example 2

[0097] The comparative example 1 provides a cosmetic containing caviar extract, whose formulation table is as follows:

[0098] Comparative example 3

[0099] The comparative example 1 provides a cosmetic containing caviar extract, whose formulation table is as follows:

[0100] Comparative example 4

[0101] The comparative example 1 provides a cosmetic containing caviar extract, whose formulation table is as follows:

[0102] Comparative example 5

[0103] The comparative example 1 provides a cosmetic containing caviar extract, whose formulation table is as follows:

[0104] Test example 1

[0105] Red blood cell hemolysis experiment:

[0106] The project evaluates the irritation through a red blood cell hemolysis experiment. The red blood cell hemolysis experiment is one of the alternative methods of the rabbit eye irritation experiment (Draize test), and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolved and the degree of denaturation. Internationally, the RBC experiment is mainly used for the eye irritation research of cosmetics and raw materials and other chemicals, and the grading standards are shown in the following table.

[0107]

[0108]

[0109] The results of the red blood cell hemolysis experiment of each group of samples of Examples 1-5 and Comparative Examples 1-5 are shown in Table 1, and L / D is the ratio of HD50 (the sample concentration when 50% of red blood cells are hemolyzed) to DI (protein denaturation index).

[0110] Table 1

[0111]

[0112] As can be seen from Table 1, the anti-aging whitening and moisturizing composition involved in the present application has the characteristics of mildness and non-irritation, and has high safety in use.

[0113] Test Example 2

[0114] Moisturizing efficacy test:

[0115] 100 volunteers uniformly clean the left and right forearm curves, and after gently absorbing the water with a paper towel, 10 people participate in the test for each group of samples, and each person in each group of samples marks 6 areas of 3cm x 3cm as test areas, and each area is at least 2cm apart, and the test results are statistically averaged. Apply an appropriate amount of equal amounts of samples of Examples 1-5 and Comparative Examples 1-5 to the test area. Before and after using the sample for 2 hours, arrange the data collection of the subjects, and each time use the skin testing instrument (Corneometer CM825) to collect skin parameters, and calculate the increase rate of the water content of the stratum corneum (%), the increase rate = (water content after 2 hours of use - water content before use) / water content before use. The results are shown in Table 2.

[0116] Table 2

[0117] Group Moisture content increase rate (%) Example 1 57.3 Example 2 46.2 Example 3 51.8 Example 4 45.5 Example 5 49.4 Comparative Example 1 18.8 Comparative Example 2 35.7 Comparative Example 3 40.1 Comparative Example 4 32.9 Comparative Example 5 54.3

[0118] From the data in Table 2, it can be seen that the composition involved in the present application has excellent moisturizing effect and can maintain the water content of the stratum corneum. Among them, the moisturizing effect of Example 1 is better than that of Examples 2-5, indicating that the preparation conditions of caviar extract significantly affect the moisturizing efficacy of the final product; the moisturizing effect of Example 1 is better than that of Comparative Examples 2-5, indicating that the four components in the composition involved in the present application have a certain synergistic effect on improving the moisturizing efficacy.

[0119] Test Example 3

[0120] DPPH radical scavenging experiment:

[0121] Accurately take 47.7 g of DPPH reagent, dilute to 100 mL in a brown volumetric flask, take 5 mL of which and dilute to 50 mL in a brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (47.7 mg / L). Respectively, 2 mL of DPPH test solution was removed, 0.1 mL of the product prepared in Examples 1-5 and Comparative Examples 1-5 was added according to the table, and then an appropriate amount of anhydrous ethanol was added to make the total mass equal. After mixing thoroughly, stand in the dark for 30 min, with anhydrous ethanol as blank control, measure the absorbance at 517 nm, and calculate the DPPH scavenging rate of each extract according to the following formula:

[0122] DPPH scavenging rate (%) = [1-(As-Ar) / A0] x 100%

[0123] In the formula: As is the absorbance of the sample and DPPH reaction system; Ar is the absorbance of the sample-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0124]

[0125] The test results are shown in Table 3:

[0126] Table 3

[0127]

[0128]

[0129] From Table 3, it can be seen that the composition involved in the present application has a good scavenging effect on DPPH, among them, the antioxidant effect of Example 1 is better than that of Examples 2-5, indicating that the preparation conditions of caviar extract significantly affect the antioxidant efficacy of the final product; the antioxidant effect of Example 1 is better than that of Comparative Examples 2-5, indicating that the four components in the composition involved in the present application have a certain synergistic effect on improving the antioxidant efficacy.

[0130] Test Example 4

[0131] The product prepared in Examples 1-5 and Comparative Examples 1-5 was subjected to tyrosinase activity inhibition test.

[0132] Test instrument and reagent:

[0133] Main instrument: spectrophotometer Spectronic GENESYS-5, Thermo, USA.

[0134] Main reagent: tyrosinase powder, enzyme activity 25000 U, Sigma, USA; L-tyrosine, analytical pure, JCBIO, Shanghai; ascorbic acid, analytical pure, Shanghai National Pharmaceutical.

[0135] Solution preparation: weigh sodium dihydrogen phosphate 7.8 g and sodium hydrogen phosphate 17.91 g, dissolve in constant volume to 500 mL with distilled water to prepare pH 6.8, 0.1 mol / L phosphate buffer; accurately measure hydrochloric acid with mass fraction of 36%-38% 0.431 mL, constant volume to 50 mL with distilled water to prepare 0.1 mol / L hydrochloric acid; weigh 0.05 g L-tyrosine and dissolve in 35 mL 0.1 mol / L hydrochloric acid, then add 65 mL PBS buffer with pH 6.8 to obtain L-tyrosine solution with mass fraction of 0.05%; dissolve tyrosinase with enzyme activity of 25000 U in 250 mL pure water to prepare tyrosinase solution with enzyme activity of 100 U / mL, divide into 1.5 mL EP tubes, 1 mL in each, and store in -40℃ refrigerator; dilute each sample and ascorbic acid with PBS buffer to make their mass fraction concentration of 5%.

[0136] Specific method: the sample system of each group is shown in the following table, first mix PBS buffer, L-tyrosine solution and each sample solution, and place in 37℃ constant temperature water bath for 10-15 min; then add tyrosinase into the reaction solution, shake well, accurately time, stop after 15 min; sequentially point on the 96-well plate, and measure the absorbance with enzyme label instrument and calculate the inhibition rate.

[0137] Reagent C1 / mL C2 / mL S1 / mL S2 / mL PBS 4 5 2 3 Sample 0 0 2 2 L-tyrosine 2 2 2 2 Tyrosinase solution 1 0 1 0

[0138] The calculation formula of tyrosinase inhibition rate is as follows:

[0139] Tyrosinase inhibition rate = [(A1-A2)-(A3-A4)] / (A1-A2) x 100%

[0140] In the formula, A1, A2, A3, A4 are the absorbance values of C1, C2, S1 and S2 respectively.

[0141] The test results are shown in Table 4:

[0142] Table 4

[0143]

[0144]

[0145] From the data in Table 4, it can be seen that the composition involved in the present application has good tyrosinase inhibitory effect, wherein the effect of Example 1 is better than that of Examples 2-5, indicating that the preparation conditions of caviar extract significantly affect the tyrosinase inhibitory efficacy of the final product; the effect of Example 1 is better than that of Comparative Examples 2-5, indicating that the four components in the composition involved in the present application have certain synergistic effect in improving the tyrosinase inhibitory effect.

[0146] The applicant declares that the anti-aging, whitening and moisturizing composition containing caviar extract and the application thereof of the present application are illustrated by the above examples, but the present application is not limited to the above examples, that is, it does not mean that the present application must rely on the above examples to be implemented. It should be understood by those skilled in the art that any improvement of the present application, equivalent replacement of each raw material of the product of the present application, addition of auxiliary ingredients, selection of specific modes, etc. fall within the protection scope and disclosure scope of the present application.

[0147] In addition, it should be noted that each specific technical feature described in the above specific embodiments can be combined in any appropriate manner without contradiction, and in order to avoid unnecessary repetition, the present application will not further illustrate various possible combination manners.

Claims

1. An anti-aging whitening moisturizing composition containing caviar extract, characterized in that, The anti-aging whitening moisturizing composition comprises 1-10 parts of sturgeon roe extract, 0.5-5 parts of salmon roe extract, 5-10 parts of N-acetylneuraminic acid and 0.5-3 parts of tranexamic acid by mass fraction; The sturgeon roe extract is prepared by a preparation method comprising the following steps: (S1) grinding the sturgeon roe and mixing with protease for enzymatic hydrolysis treatment, centrifuging the enzymatic hydrolysate and taking the supernatant; (S2) mixing the supernatant with phosphoric acid for degumming treatment, centrifuging and taking the supernatant; (S3) mixing the supernatant with alkali for deacidification treatment, centrifuging and taking the supernatant; (S4) performing decolorization and deodorization treatment on the supernatant to obtain the sturgeon roe extract; The protease in step (S1) is a combination of papain and subtilisin with an enzyme activity ratio of 1:1; The addition amount of the protease in step (S1) is 3000-5000 U / g; The enzymatic hydrolysis treatment in step (S1) is performed at pH=6-8 and a temperature of 36-45℃ for 3-8 h; The salmon roe extract is prepared by a preparation method comprising the following steps: (T1) grinding the salmon roe and mixing with protease for enzymatic hydrolysis treatment, centrifuging the enzymatic hydrolysate and taking the supernatant; (T2) mixing the supernatant with phosphoric acid for degumming treatment, centrifuging and taking the supernatant; (T3) mixing the supernatant with alkali for deacidification treatment, centrifuging and taking the supernatant; (T4) performing decolorization and deodorization treatment on the supernatant to obtain the salmon roe extract; The protease in step (T1) is a combination of papain and neutral protease with an enzyme activity ratio of 1:1; The addition amount of the protease in step (T1) is 1000-3000 U / g; The enzymatic hydrolysis treatment in step (T1) is performed at pH=6-8 and a temperature of 35-40℃ for 2-5 h.

2. The caviar extract-containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The addition amount of the phosphoric acid in step (S2) is 1.0-2.0% of the supernatant.

3. The caviar extract-containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The degumming treatment is performed at 60-70℃ for 0.5-2 h.

4. The caviar extract-containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The addition amount of the alkali in step (S3) is 1.0-2.0% of the supernatant.

5. The caviar extract-containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The deacidification treatment is performed at 50-60℃ for 0.5-2 h.

6. The caviar extract-containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The centrifugation in steps (S2), (S3), (T2) and (T3) is independently performed at 3000-5000 rpm for 5-15 min.

7. The caviar extract containing anti-aging whitening moisturizing composition according to claim 1, characterized by, The addition amount of the phosphoric acid in step (T2) is 0.5-1.0% of the supernatant.

8. The caviar extract containing anti-aging whitening moisturizing composition according to claim 1, characterized in that, The addition amount of the alkali in step (T3) is 0.5-1.0% of the supernatant.

9. Use of the fish roe extract-containing anti-aging whitening moisturizing composition according to any one of claims 1-8 in the preparation of a cosmetic product.

10. Use according to claim 9, characterized in that, The cosmetic product comprises lotion, cosmetic water, cream, mask, makeup remover, facial cleanser.

11. Use according to claim 9, characterized in that, The content of the anti-aging whitening moisturizing composition in the cosmetic product is 1-10%.

Images