Composition with thickening effect, amino acid surfactant composition and use of biosurfactants

Abstract

This application discloses a thickening composition and an amino acid surfactant, including a lipopeptide biosurfactant. The lipopeptide biosurfactant possesses the basic cleaning and foaming properties of surfactants while thickening the amino acid surfactant and its compound system, synergistically improving cleaning ability without affecting the system's appearance or transparency. The lipopeptides in this application are produced by bacteria, are a green source of raw materials, are mild and non-irritating, harmless to humans and environmentally friendly, biodegradable, and pose no harm with long-term use.

Description

Technical Field

[0001] This application relates to the field of daily chemical technology, and more specifically, to the use of compositions with thickening properties, amino acid surfactant compositions, and biosurfactants. Background Technology

[0002] Surfactants play a crucial role in cosmetics, acting as emulsifiers, solubilizers, dispersants, and cleaners. Their cleaning properties are primarily found in facial cleansers, shampoos, and shower gels. Traditional surfactants, such as sulfate-based and soap-based surfactants, are widely used in various cleaning and hair care products due to their strong cleaning and degreasing power. However, these two types of surfactants can irritate the skin and hair with prolonged use. Amino acid surfactants, based on biomass, possess an amino acid backbone structure, making them compatible with skin and hair. In addition to the general properties of surfactants, they are milder, have lower toxicity and side effects, are less irritating, and are readily biodegradable. They represent an upgrade from traditional surfactants and are increasingly used in cleaning and hair care products. However, amino acid surfactants are difficult to thicken, and their addition can even lead to rapid viscosity reduction in the system, thus limiting their application in cosmetics.

[0003] The most commonly used amino acid surfactants in cosmetics are N-acyl amino acid surfactants. Based on the type of hydrophilic amino acid, they are classified into glycine, glutamic acid, sarcosine, alanine, and taurine types; based on the lipophilic group, they are classified into coconut oil-based, lauryl (C12), myristyl (C14), palmityl (C16), and stearyl (C18); and based on the inorganic salt, they are classified into sodium salts, potassium salts, and TEA salts, etc. The difficulty in thickening amino acid surfactants is due to their relatively large hydrophilic groups, which make it difficult to form rod-shaped micelles. Therefore, the hydrophilic group is the key factor affecting the thickening ease of amino acid surfactants.

[0004] Currently, the main methods for thickening amino acid surfactants include: 1) using polymer thickeners, such as acrylate copolymers and cellulose; 2) compounding nonionic (such as fatty alcohols, fatty acids, alkanolamides, alkyl glycosides and esters), cationic, and amphoteric surfactants (such as betaines); 3) adding natural gums and their modifiers, such as carrageenan, guar gum, and xanthan gum. Each of these thickening methods has its own problems or is only applicable to a few amino acid surfactants. Using polymer thickeners can lead to problems such as easy discoloration at high temperatures, jelly-like phenomena at low temperatures, reduced foaming effect and user experience, and a slippery feel due to incomplete rinsing. Furthermore, polymers typically require very high dosages to achieve the desired thickening effect. Compounding other surfactants for thickening not only has selectivity for amino acid surfactants and is not suitable for multiple amino acid surfactants, but also places high demands on the type and ratio of the compounded surfactants, significantly limiting the use of amino acid surfactants in formulations. In addition, excessive addition of some nonionic surfactants can reduce the transparency of the system. Gel-like substances are not only difficult to dissolve, but excessive amounts can also cause problems such as jelly-like texture, stringiness, and poor rheological properties. In addition, the amount used is usually low, serving as an auxiliary thickening ingredient.

[0005] Therefore, there is an urgent need in this field for a thickener that can efficiently thicken a variety of amino acid surfactants, is not limited by the formulation system of the application, has no negative effects on the system, and does not affect the intended use of amino acid surfactants (mild and low-irritant, green and environmentally friendly, with good foaming and cleaning power). Summary of the Invention

[0006] To address the problems existing in the prior art, this application provides a high-efficiency, widely applicable, environmentally friendly, mild and low-irritant thickener composition for thickening amino acid surfactants and its application in cosmetics.

[0007] Specifically, this application adopts the following technical solution:

[0008] 1. A composition having a thickening effect, wherein it comprises 0.0001 wt% to 99.9999 wt% of a biosurfactant as the thickening active ingredient.

[0009] 2. The composition according to claim 1, wherein the thickening is a thickening of an amino acid surfactant.

[0010] 3. The composition according to item 1 or 2, wherein the biosurfactant is the sole active ingredient for thickening the amino acid surfactant.

[0011] 4. The composition according to any one of items 1-3, wherein the biosurfactant is selected from one or more of lipopeptides, glycolipids and phospholipids.

[0012] 5. The composition according to item 4, wherein the lipopeptide is selected from one or more of the group consisting of: surfactants, irisin, fenbufen, lichenin, babylin, permiracin, spore-forming fungicides, antifungal fungicides, phosphatidylin, fusarium fungicides, kurstakin, spore-forming fungicides, polymyxins, octapeptide, and polypeptide fungicides.

[0013] 6. The composition according to any one of items 2-5, wherein the amino acid surfactant is selected from one or more of glycine type, sarcosine type, glutamic acid type, alanine type or taurine type.

[0014] 7. The composition according to any one of items 2-6, wherein the hydrophilic amino acid of the amino acid surfactant has a molecular weight of less than 150 Da.

[0015] 8. The composition according to any one of items 2-7, wherein the mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:50, preferably 1:5 to 1:30.

[0016] 9. The use of the composition having a thickening effect as described in any one of items 1-8, wherein the composition having a thickening effect is added as a thickener to a cosmetic.

[0017] 10. An amino acid surfactant composition, comprising an amino acid surfactant and the composition having a thickening effect as described in any one of items 1-9.

[0018] 11. The composition according to item 10, wherein the content of the biosurfactant is 5 wt% to 90 wt%, preferably 10 wt% to 50 wt%, based on the weight of the amino acid surfactant composition.

[0019] 12. The composition according to item 10 or 11, further comprising other surfactants besides amino acid surfactants, wherein the other surfactants are preferably one or more of anionic surfactants, nonionic surfactants or amphoteric surfactants.

[0020] 13. The composition according to any one of items 10-12, wherein the composition comprises one or more of water, inorganic salts, other surfactants, humectants, pH adjusters, and preservatives.

[0021] 14. The composition according to any one of items 10-13, wherein the inorganic salt is sodium chloride, preferably, the mass ratio of sodium chloride to amino acid surfactant is 1:10 to 1:30.

[0022] 15. Use of biosurfactants in amino acid surfactants, wherein the biosurfactants act as thickeners in the amino acid surfactants.

[0023] 16. The composition according to items 10-14 or the use according to item 15, wherein the biosurfactant is selected from one or more of lipopeptides, glycolipids and phospholipids.

[0024] 17. The composition or use according to claim 18, wherein the lipopeptide is selected from one or more of the group consisting of: surfactants, iturobrine, fenbufen, lichenin, babylin, permiracin, spore-forming mycotoxin, antifungal mycotoxin, phosphatidylcholine, fusarium oxysporin, kurstakin, spore-forming mycotoxin, polymyxin, octapeptide, and polypeptide mycotoxin.

[0025] 18. The composition according to items 10-14 or the use according to any one of items 15-17, wherein the amino acid surfactant is selected from one or more of glycine type, sarcosine type, glutamic acid type, alanine type or taurine type.

[0026] 19. The composition described in items 10-14 or the use described in any one of items 15-18, wherein the hydrophilic amino acid of the amino acid surfactant has a molecular weight of less than 150 Da.

[0027] 20. The composition according to items 10-14 or the use according to any one of items 15-19, wherein the mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:50, preferably 1:5 to 1:30.

[0028] Invention Effects

[0029] 1. The lipopeptides in this application are produced by bacteria, are green source raw materials, are mild and non-irritating, harmless to the human body and environmentally friendly, biodegradable, and have no harm with long-term use;

[0030] 2. The thickening composition in this application has good electrolyte resistance, does not require neutralization, and is less affected by temperature;

[0031] 3. The lipopeptide in this application is a biosurfactant that possesses the basic properties of surfactants such as cleaning and foaming, while also thickening amino acid surfactants and their compound systems to synergistically improve cleaning ability without affecting the appearance or transparency of the system. Detailed Implementation

[0032] The following description provides exemplary embodiments of this application, including various details to aid understanding, and should be considered merely exemplary. Therefore, those skilled in the art will recognize that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of this application. Similarly, for clarity and brevity, descriptions of well-known functions and structures are omitted in the following description.

[0033] This application provides a composition having a thickening effect, wherein 0.0001 wt% to 99.9999 wt% of a biosurfactant is included as the thickening active ingredient.

[0034] In this application, "thickening" is used.

[0035] In one specific embodiment, in the composition having a thickening effect, the biosurfactant is the sole active ingredient for thickening the amino acid surfactant. "Sole active ingredient" means that the composition having a thickening effect does not contain any other thickening ingredients.

[0036] The biosurfactants of this application are surface-active metabolites produced by microorganisms during metabolism under specific culture conditions, including lipopeptides, glycolipids, and phospholipids.

[0037] The lipopeptides of this application may be cyclic or linear lipopeptides, preferably having one of the following formulas (I)-(III):

[0038]

[0039]

[0040] In this structure, R represents the carbon chain of the fatty acid chain, and A1, A2, A3…Am represent the 1st, 2nd, 3rd…mth amino acids on the peptide chain, respectively. The carboxyl group of the fatty acid is attached to the N-terminus of A1, and the C-terminal carboxyl group of Am is attached to the hydroxyl or amino groups of the fatty acid or other amino acids on the peptide chain to form a cyclic structure. Linear lipopeptides are linear products formed after the ring-opening of cyclic lipopeptides.

[0041] Lipopeptides are metabolites produced during microbial fermentation. They are amphoteric substances composed of hydrophilic cyclic oligopeptides and hydrophobic fatty acid chains linked by lactone bonds. Primarily derived from microorganisms such as Bacillus and Streptomyces, they have asymmetrical molecular structures, strong polarity, and are amphiphilic (both water and oil-loving). They can significantly reduce surface and interfacial tension, making them a novel type of natural surfactant. These surfactants mainly include surfactants such as iturins and fengycin. Surfactins consist of a 12-17 carbon atom β-hydroxy fatty acid linked to a 7-α amino acid cyclic heptapeptide linked by lactone bonds. Fengycin consists of a 14-17 carbon atom β-hydroxy fatty acid linked to a 10-amino acid cyclic peptide chain; the cyclic peptide structure of fengycin is formed by the peptide chain itself, without the β-hydroxy fatty acid participating. Iturins consist of a β-amino fatty acid and a peptide chain linked by amide bonds. Most bacterial metabolic products, such as ituronic acid, have a heptapeptide peptide chain that forms a macrocycle with a β-amino fatty acid containing 14-17 carbon atoms. Lipopeptides have extremely low CMC values, ranging from 15-25 mg / L, and can reduce the surface tension of water to as low as 28 mN / m. Lipopeptide biosurfactants possess excellent emulsifying, dispersing, and detergency properties, are biodegradable, and exhibit low toxicity and low irritation.

[0042] The lipopeptide can be any type of lipopeptide known in the art, such as those from the surfactant family, the iturobrine family, and the fenbufen (also known as fenbufen) family.

[0043] In one specific embodiment, the lipopeptide is selected from one or more of the following groups: surfactant, irisin, fenbufen, lichenin, babylin, permiracin, spore-forming mycotoxin, antifungal mycotoxin, phosphatidylcholine, fusarium oxytocin, kurstakin, spore-forming mycotoxin, polymyxin, octapeptide, and polypeptide mycotoxin.

[0044] In one specific embodiment, the lipopeptide is a surfactant.

[0045] In one specific embodiment, the lipopeptide is itursin.

[0046] In one specific embodiment, the lipopeptide is fenestrated mustard.

[0047] The lipopeptide can be produced by bacteria, which can be wild-type or genetically engineered bacteria. For example, the bacteria can be selected from the group consisting of Bacillus, Pseudomonas, Streptomyces, and Arthrobacter.

[0048] In one specific embodiment, the lipopeptide is obtained by culturing Bacillus.

[0049] In one specific embodiment, the lipopeptide is obtained by culturing Pseudomonas bacteria.

[0050] In one specific embodiment, the lipopeptide is obtained by culturing Bacillus and regulating the expression of lipopeptide synthesis-related genes. These genes are selected from one or more of the following groups: lipopeptide synthesis gene srfA or fen (a fen-like substance), lic (a lichenin-like substance), bam (a spore-forming mycotoxin-like substance), mycotoxin-like substance, itu (a spore-forming mycotoxin-like substance), pps (a phosphatidylin-like substance), fusin (a fusin-like substance), krs (a spore-forming mycotoxin-like substance), pmx (a polymyxin-like substance), octapeptide-like substance, ycxA (a transmembrane transport protein-like substance), yngH (a biotin carboxylase-like substance), spore synthesis genes spoIVA / B / C / F, spoVA / B / D / E, and leucine synthesis pathway genes leuABCD / ilvK. The glycolipids of this application include various types of glycolipids produced by microbial fermentation, such as rhamnolipids, sophorolipids, and mannose-erythritol lipolipids.

[0051] In one specific embodiment, the glycolipid is selected from one or more of the following groups: rhamnolipid, sophorolipid, mannose erythritol lipolipid, trehalose lipolipid, and cellobiose lipolipid.

[0052] In one specific embodiment, the glycolipid is rhamnolipid.

[0053] In one specific embodiment, the glycolipid is sophorolipid.

[0054] The phospholipids in this application include various types of phospholipids produced by microbial fermentation, which are divided into two major categories: glycerol phospholipids and neurol phospholipids, such as lecithin, cephalin, inositol phospholipids, serine phospholipids, and phosphoryl ethanolamine.

[0055] In one specific embodiment, the phospholipid is phosphorylethanolamine.

[0056] Those skilled in the art will understand that the biosurfactants of this application can be obtained commercially or cultivated in-house. For example, the lipopeptides of this application can be produced by fermentation using Bacillus subtilis THY-7 / Pg3-srfA constructed in CN201810865295.7. Specifically, the fermentation broth obtained by directly fermenting Bacillus subtilis THY-7 / Pg3-srfA can be used as a composition with thickening properties; or further, the pH of the fermentation broth can be adjusted to acidity, causing the lipopeptide surfactant to precipitate, followed by solid-liquid separation and drying to obtain a product containing lipopeptide surfactant as a composition with thickening properties. The content of surfactant in the composition can be above 70 wt%, for example, 70 wt%-99 wt% or 95 wt%-99 wt%. The content of biosurfactant in the thickening composition of this application is 0.0001 wt% to 99.9999 wt%, for example, it can be 0.0001 wt%, 0.0002 wt%, 0.0005 wt%, 0.001 wt%, 0.005 wt%, 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.2 wt%, 0.4 wt%, 0.6 wt%, 0.8 wt%, 1.0 wt%, 1.5 wt%, 2.0 wt%, 2.5 wt%, 3.0 wt%, 4.0 wt%, 5.0 wt%, 10 wt%, 15 wt%, 20 wt%, 30 wt%, 40 wt%, 50 wt%, 60 wt%, 70 wt%, 80 wt%, 90 wt%, 95 wt%, 99 wt%, 99.9999 wt%, or any range between these values.

[0057] The thickening composition provided in this application not only has a good thickening effect on amino acid surfactants, but can also effectively thicken various amino acid surfactants and their compound systems, including but not limited to glycine-type, sarcosine-type, glutamic acid-type, alanine-type, and taurine-type. It has no selectivity for amino acid surfactants, is not affected by other components in the system, and does not affect the excellent properties of amino acid surfactants (such as mildness, low irritation, foaming, etc.) and the transparency and stability of the system. It is suitable for various dosage forms and formulation systems.

[0058] In a preferred embodiment, depending on the type of hydrophilic amino acid in the amino acid surfactant, the smaller the molecular weight of the hydrophilic amino acid, the better the thickening effect of the composition with thickening effect provided in this application. Examples of hydrophilic amino acids with small molecular weight include glutamic acid (molecular weight 147.13), alanine (molecular weight 89.09), sarcosine (molecular weight 89.09), glycine (molecular weight 75.07), etc. In this application, the specific type of hydrophilic amino acid is not limited. In a further preferred embodiment, the hydrophilic amino acid of the amino acid surfactant has a molecular weight of less than 150 Da. For example, the molecular weight can be less than 140 Da, less than 130 Da, less than 120 Da, less than 110 Da, less than 100 Da, less than 90 Da, less than 80 Da, less than 70 Da, less than 60 Da, less than 50 Da, or less than 40 Da. Examples of amino acid surfactants with a hydrophilic amino acid molecular weight of less than 150 Da include sodium cocoyl glutamate (glutamic acid molecular weight is 147.13), sodium cocoyl aminopropionate (alanine molecular weight is 89.09), sodium lauroyl sarcosinate (sarcosine molecular weight is 89.09), sodium cocoyl glycinate (glycine molecular weight is 75.07), sodium methyl cocoyl taurate (taurate molecular weight is 125.15), etc. The types of amino acid surfactants with a hydrophilic amino acid molecular weight of less than 150 Da in this application are not limited.

[0059] When the thickening composition of this application is used to thicken an amino acid surfactant, the amounts of the biosurfactant and the amino acid surfactant are not limited. In a preferred embodiment, the mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:50, for example, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:15, 1:20, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:35, 1:40, 1:45, 1:49, and more preferably 1:5 to 1:30.

[0060] The thickening composition of this application may further include one or more of water, inorganic salts, surfactants, humectants, pH adjusters, and preservatives.

[0061] The thickening composition of this application may further include a surfactant, which may be one or more of anionic surfactants, nonionic surfactants, amphoteric surfactants, and amino acid surfactants. The anionic surfactants include, but are not limited to, one or more of fatty acid soaps, carboxylates, sulfates (esters), sulfonates (esters), and phosphates (esters); the nonionic surfactants include, but are not limited to, fatty alcohols, fatty acids, alkanolamides, glycosides, and esters; the amphoteric surfactants include, but are not limited to, betaines, imidazoline types, and amine oxide types; and the amino acid surfactants include, but are not limited to, N-acyl amino acids, N-alkyl amino acids, and amino acid esters. Mixtures of surfactants may be used. When a surfactant is present in the thickening composition of this invention, its content may be 0.0001 wt% to 99 wt%, preferably 30 wt% to 80 wt%.

[0062] When the thickening composition of this application contains a surfactant, such as an amino acid surfactant, the thickening composition can be self-thickening or used to thicken other amino acid surfactants.

[0063] This application also provides the application of the composition having a thickening effect, adding the composition as a thickener to cosmetics, preferably cleansing cosmetics, such as facial cleansers, cleansing gels, shampoos, and shower gels. Preferably, the cleansing cosmetic formulation uses an amino acid surfactant as the main surfactant or contains an amino acid surfactant.

[0064] This application further provides an amino acid surfactant composition, comprising an amino acid surfactant and a composition having a thickening effect as described in any of the preceding claims.

[0065] The amounts of the biosurfactant and the amino acid surfactant are not limited. In a preferred embodiment, the mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:50, for example, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:15, 1:20, 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:35, 1:40, 1:45, 1:49, and more preferably 1:5 to 1:30. In a preferred embodiment, the content of the biosurfactant, based on the weight of the amino acid surfactant composition, is 5 wt% to 90 wt%, for example, it can be 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, 15 wt%, 20 wt%, 25 wt%, 30 wt%, 35 wt%, 40 wt%, 45 wt%, 46 wt%, 47 wt%, 48 wt%, 49 wt%, 50 wt%, 55 wt%, 60 wt%, 65 wt%, 70 wt%, 75 wt%, 80 wt%, 85 wt%, or 89 wt%, and more preferably 10 wt% to 50 wt%.

[0066] The amino acid surfactant composition may further contain other surfactants besides the amino acid surfactant, preferably one or more of anionic surfactants, nonionic surfactants, or zwitterionic surfactants. The anionic surfactants include, but are not limited to, one or more of fatty acid soaps, carboxylates, sulfates (esters), sulfonates (esters), and phosphates (esters); the nonionic surfactants include, but are not limited to, fatty alcohols, fatty acids, alkanolamides, glycosides, and esters; the zwitterionic surfactants include, but are not limited to, betaines, imidazoline-type surfactants, and amine oxide-type surfactants. Mixtures of surfactants may be used. When other surfactants are present in the composition having a thickening effect, their content may be 0.0001 wt% to 99.9 wt%, preferably 30 wt% to 80 wt%.

[0067] When inorganic salts are present in the composition having a thickening effect, the inorganic salts include, but are not limited to, sodium chloride, potassium chloride, and ammonium chloride. Mixtures of inorganic salts can be used. Their content can be 0-80 wt%, preferably not more than 50 wt%, preferably not more than 30 wt%, preferably not more than 20 wt%, and preferably not more than 10 wt%.

[0068] In a preferred embodiment, the inorganic salt is sodium chloride, used as a stabilizer and auxiliary thickener. In a further preferred embodiment, the mass ratio of sodium chloride to amino acid surfactant is 1:10 to 1:30, for example, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28, or 1:29. In a further preferred embodiment, when the composition is used to prepare an aqueous solution, the sodium chloride does not exceed 3 wt% of the total volume of the aqueous solution system.

[0069] When a humectant is present in a composition having a thickening effect, the humectant may be one or more of polyol humectants, polyether humectants, polysaccharide humectants, and amino acid humectants; the polyol humectants include, but are not limited to, butylene glycol, glycerol, 1,2-pentanediol, polypropylene glycol, sorbitol, and pentanediol; the polysaccharide humectants include, but are not limited to, hydroxyethyl urea, soluble proteoglycans, sodium hyaluronate, tremella polysaccharide, and xylitol; the polyether humectants include, but are not limited to, glyceryl polyoxyethylene ether, polyoxypropylene ether, and polyoxyethylene-polyoxypropylene copolymer.

[0070] When a pH adjuster is present in a composition having a thickening effect, the pH adjuster includes, but is not limited to, triethanolamine, octanoyl hydroxamic acid, sodium hydroxide, sodium bicarbonate, citric acid, and sodium citrate, and may be one or more of the above-mentioned pH adjusters.

[0071] When a preservative is present in a composition having a thickening effect, the preservative includes, but is not limited to, imidazolidinyl urea, DMDM ​​hydantoin, isothiazolinone, bropol, phenoxyethanol, benzyl alcohol, parabens, benzoic acid, and sorbic acid, and may be one or more of the above preservatives.

[0072] Regarding the specific types and amounts of water, inorganic salts, surfactants, humectants, pH adjusters, and preservatives, those skilled in the art can make corresponding adjustments based on the composition of existing amino acid surfactant products and the specific product form of the amino acid surfactant. Those skilled in the art will understand that the surfactants referred to here are surfactants other than the biosurfactants of this application.

[0073] This application further provides the use of any of the biosurfactants described above in amino acid surfactants, wherein the biosurfactant acts as a thickener in the amino acid surfactant.

[0074] In the use of the composition having a thickening effect in cosmetics and in the use of the biosurfactant in an amino acid surfactant, the biosurfactant is any of the biosurfactants described above, the amino acid surfactant is any of the amino acid surfactants described above, and the mass ratio of the biosurfactant to the amino acid surfactant is the mass ratio of any of the biosurfactants to the amino acid surfactants described above.

[0075] The thickening composition described in this application not only has a good thickening effect on amino acid surfactants, but can also effectively thicken various amino acid surfactants and their compound systems, including but not limited to glycine-type, sarcosine-type, glutamic acid-type, alanine-type, and taurine-type. It has no selectivity for amino acid surfactants, is not affected by other components in the system, and does not affect the excellent properties of amino acid surfactants (such as mildness, low irritation, foaming, etc.) and the transparency and stability of the system. It is suitable for various dosage forms and formulation systems.

[0076] Example

[0077] Example 1: Preparation of lipopeptides

[0078] 1. Obtaining a composition with thickening effect

[0079] A single colony of Bacillus subtilis THY-7 / Pg3-srfA (obtained from patent CN109097315A) was picked, inoculated into LB liquid medium, and cultured at 37℃ and 200rpm for 16 hours to obtain seed culture. The seed culture was then inoculated into a shake flask of fermentation medium at a ratio of 5%, and cultured at 37℃ and 200rpm for 2-6 hours. 1 Mm IPTG was added, and the culture was continued for 2 days to obtain the fermentation broth containing surfactant.

[0080] The fermentation medium used consisted of: 30-100 g / L sugars, 10-50 g / L inorganic nitrogen source, 0.5-3 g / L organic nitrogen source, 0.1-1 g / L KH2PO4, 0.5-0.3 g / L Na2HPO4·12H2O, 0.002-0.01 g / L CaCl2, 0.002-0.01 g / L MnSO4·H2O, 0.002-0.01 g / L FeSO4·7H2O, pH 6.5-7.5, and fermentation additives (silicone defoamers, polysaccharides, amino acids or their derivatives, oligopeptides, phospholipids, glycolipids, and / or fatty acids and their derivatives) 0.01-20 wt%.

[0081] The surfactant content in the fermentation broth was determined using the method disclosed in CN105400784A. Specifically, 1 mL of fermentation broth was centrifuged at 12000 rpm for 1 minute, and 100 μL of the supernatant was added to 1900 μL of deionized water. After mixing, the mixture was filtered through a 0.22 μm filter and analyzed by HPLC. The mobile phase for HPLC analysis was methanol and water in a ratio of 85 / 15, the flow rate was 1 mL / min, the chromatographic column was a C18-ODS reversed-phase column, the column temperature was 40℃, and the detection wavelength was 205 nm using a UV detector.

[0082] The content of surfactant in the fermentation broth was found to be approximately 10-50 g / L. Surfactant was extracted from the fermentation broth and obtained as a powder by freeze-drying. The content of lipopeptide in the obtained powder was 40 wt%-99.99 wt%. The powder was purified to obtain purified lipopeptide powder with a lipopeptide content of 95 wt%-99.99 wt%. The lipopeptide powder is composition 1 of this application.

[0083] 2. Obtaining a composition containing iturobrine and fenbufen

[0084] Iridin and fenbufen were purchased from MedChemExpress and were used as Composition 2 and Composition 3 of this application, respectively, wherein the lipopeptide content was 99wt%-99.99wt%.

[0085] Example 2: Thickening experiment with a single amino acid surfactant

[0086] Because there are many types of amino acid surfactants, and because their thickening properties are difficult due to the relatively large hydrophilic groups (amino acids) that make it hard to form rod-shaped micelles, the type of hydrophilic group, i.e., the amino acid, is a key factor affecting the thickening effect of amino acid surfactants. Based on the type of amino acid, amino acid surfactants are classified into glycine-type, glutamic acid-type, sarcosine-type, alanine-type, and taurine-type. Therefore, this application only selects one of each of the above five types of surfactants as an example for illustration. The selected amino acid surfactants are: potassium cocoyl glycinate, sodium cocoyl glutamate, sodium lauroyl sarcosinate, sodium cocoaminopropionate, and sodium methyl cocoyl taurate. In this application, the amino acid surfactants used are all raw materials with a content of 30% or 35% for formulation experiments.

[0087] In this embodiment, the thickening compositions 1, 2, and 3 from Example 1 (hereinafter referred to as thickener compositions) were used to conduct thickening tests on potassium cocoyl glycinate, sodium cocoyl glutamate, sodium lauroyl sarcosinate, sodium cocoyl aminopropionate, and sodium methyl cocoyl taurate, respectively. Different amounts of thickener compositions 1, 2, and 3 were added to the basic formulation containing the above-mentioned amino acid surfactants, and the viscosity was tested using a rotational viscometer.

[0088] 2.1 Thickening properties of the thickener composition on potassium cocoyl glycinate

[0089] The basic formulation containing potassium cocoyl glycinate is shown in Table 1, and the amount of thickener composition added and the tested viscosity are shown in Table 2.

[0090] Table 1. Basic formulations containing potassium cocoyl glycinate

[0091]

[0092] Table 2 Thickening properties of thickener compositions on potassium cocoyl glycinate

[0093] Amount of Composition 1 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 4687 19800 28000 32400 33600 Formula 2 viscosity, MPAs 6450 22100 36400 37500 40000 Amount of Composition 2 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 4687 16470 27320 33190 33410 Formula 2 viscosity, MPAs 6450 20300 35700 37400 39700 Amount of Composition 3 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 4687 15587 21750 27610 31190 Formula 2 viscosity, MPAs 6450 19800 31670 33400 37800

[0094] 2.2 Thickening properties of the thickener composition on sodium cocoyl glutamate

[0095] The basic formulation containing sodium cocoyl glutamate is shown in Table 3, and the amount of thickener composition added and the tested viscosity are shown in Table 4.

[0096] Table 3 Basic formulations containing sodium cocoyl glutamate

[0097]

[0098]

[0099] Table 4 Thickening properties of thickener compositions on sodium cocoyl glutamate

[0100] Amount of Composition 1 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 57 103 295 517 961 Formula 2 viscosity, MPAs 83 167 325 570 1366 Amount of Composition 2 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 57 135 301 696 1591 Formula 2 viscosity, MPAs 83 231 497 823 1820 Amount of Composition 3 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 57 108 233 501 977 Formula 2 viscosity, MPAs 83 132 295 514 1130

[0101] 2.3 Thickening properties of the thickener composition on sodium lauroyl sarcosinate

[0102] The basic formulation containing sodium lauroyl sarcosinate is shown in Table 5, and the amount of thickener composition added and the tested viscosity are shown in Table 6.

[0103] Table 5 Basic formulations containing sodium lauroyl sarcosinate

[0104]

[0105]

[0106] Table 6 Thickening properties of thickener compositions on sodium lauroyl sarcosinate

[0107] Amount of Composition 1 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 264 700 1671 2250 3345 Formula 2 viscosity, MPAs 336 801 1961 2875 4616 Amount of Composition 2 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 264 639 1653 2520 3475 Formula 2 viscosity, MPAs 336 786 1930 3133 3928 Amount of Composition 3 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 264 701 1526 2210 3593 Formula 2 viscosity, MPAs 336 832 1855 2950 4155

[0108] 2.4 Thickening properties of the thickener composition for sodium methylcocoyl taurate

[0109] The basic formulation containing sodium methyl cocoyl taurate is shown in Table 7, and the amount of thickener composition added and the tested viscosity are shown in Table 8.

[0110] Table 7 Basic Formulations Containing Sodium Methylcocoyl Taurate

[0111]

[0112] Table 8 Thickening properties of thickener compositions on sodium methylcocoyl taurate

[0113] Amount of Composition 1 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 91 605 1231 1973 2674 Formula 2 viscosity, MPAs 106 703 1524 2346 3150 Amount of Composition 2 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 91 599 1344 1891 2595 Formula 2 viscosity, MPAs 106 695 1690 2100 2928 Amount of Composition 3 added 0 0.5wt% 0.8wt% 1.0wt% 1.5wt% Formula 1 viscosity, MPa 91 571 1203 1795 2681 Formula 2 viscosity, MPAs 106 710 1531 2360 3155

[0114] 2.5 Thickening properties of the thickener composition on sodium cocoaminopropionate

[0115] The basic formulation containing sodium cocoyl aminopropionate is shown in Table 9, and the amount of thickener composition added and the tested viscosity are shown in Table 10.

[0116] Table 9 Basic Formulations Containing Sodium Cocoylaminopropionate

[0117]

[0118] Table 10 Thickening properties of thickener compositions on sodium cocoyl aminopropionate

[0119]

[0120]

[0121] The data in the table above shows that thickener compositions 1, 2, and 3 have good thickening effects on the five types of amino acid surfactants tested, and the dosage is relatively low. After adding the thickener compositions to the test formulations, the viscosity of the system increases, and the appearance becomes clear and transparent. They also have a significant thickening effect on glutamic acid-type surfactants, which are difficult to thicken. In the thickening tests, the addition amounts of compositions 1, 2, and 3 were 0.5 wt%, 0.8 wt%, 1.0 wt%, and 1.5 wt%, respectively. Adding 0.5 wt% of the thickener composition to the test formulations resulted in a significant increase in viscosity, and the viscosity increased with increasing thickener composition dosage.

[0122] Example 3: Thickening experiment of amino acid surfactant compound system

[0123] This embodiment tests the thickening performance of thickener composition 1, thickener composition 2, and thickener composition 3 of this application on an amino acid surfactant compound system. Different amounts of thickener composition 1, thickener composition 2, and thickener composition 3 were added to the above basic test formulation, and the viscosity of the system was tested using a rotational viscometer.

[0124] Table 11 shows the compound system 1 of various amino acid surfactants, and Table 12 shows the compound system 2 of amino acid surfactants with anionic, amphoteric, and nonionic surfactants. The amount of thickener composition added and the tested viscosity are shown in Table 13.

[0125] Table 11 Amino acid surfactant compound system 1

[0126]

[0127]

[0128] Table 12 Amino acid surfactant compound system 2

[0129]

[0130] Table 13 Thickening test results of amino acid surfactant compound systems

[0131] Composition and Dosage The viscosity of compound system 1, mpas The viscosity of compound system 2, mpas No added composition 95 127 Composition 1, 0.5 wt% 803 982 Composition 1, 1.0 wt% 1970 2350 Composition 2, 0.5 wt% 795 863 Composition 2, 1.0 wt% 1890 1985 Composition 3, 0.5 wt% 701 1032 Composition 3, 1.0 wt% 1540 2565

[0132] The data in the table show that thickener composition 1, thickener composition 2, and thickener composition 3 not only have good thickening effects on single amino acid surfactants, but also have very good thickening effects on compound systems of multiple amino acid surfactants (compound system 1) and compound systems of amino acid surfactants with nonionic, amphoteric, and anionic surfactants (compound system 2).

[0133] Example 4: Transparent Amino Acid Facial Cleansing Gel

[0134] In this embodiment, a transparent amino acid surfactant system cleansing gel was prepared using composition 1 of Example 1. The raw material composition and formulation are shown in Table 14.

[0135] Table 14. Ingredients and Proportions of Transparent Amino Acid Facial Cleanser

[0136] Raw material name Content, wt% (based on active ingredient content) Potassium cocoyl glycinate 5 Sodium cocoyl glutamate 2 Sodium lauroyl sarcosinate 5 Alkyl glycosides 3.5 Sodium lauroylamphoacetate 2 Cocamidopropyl Betaine 2.5 glycerin 5 Citric acid Add an appropriate amount and adjust the pH to 6.0-7.0. EDTA-2Na 0.1 Composition 1 2.0 essence 0.3 preservative Appropriate amount water Up to 100

[0137] The preparation method is as follows:

[0138] 1. Add 50% deionized water and EDTA2Na to the stirred tank and stir until homogeneous;

[0139] 2. Add potassium cocoyl glycinate, sodium cocoyl glutamate, sodium lauroyl sarcosinate, alkyl glycoside, sodium lauroamphoacetate, and cocamidopropyl betaine to a mixing tank and stir thoroughly until homogeneous;

[0140] 3. Add glycerin and stir well;

[0141] 3. Add an appropriate amount of citric acid to adjust the pH to 6.0-7.0;

[0142] 4. Add the composition 1 of Example 2, the fragrance, and the preservative, and stir until well mixed;

[0143] 5. Add the remaining deionized water to bring the total to 100%;

[0144] 6. Once the sample passes the inspection, the material can be discharged.

[0145] The facial cleansing gel of this embodiment uses amino acid surfactants as the main surfactant. It is colorless, clear, and transparent in appearance, without any suspended or precipitated impurities. It contains no other thickeners besides thickener composition 1. Its viscosity is 1800-2000 mPa·s (25°C), and its pH is 6.0-7.0 (slightly acidic). It exhibits good cleansing and foaming power and has no odor other than added fragrance. After temperature resistance (40°C ± 2°C) and cold resistance (-5°C ± 2°C) tests, the product showed no change in color or odor, and no suspended matter, sediment, or crystallization. All product indicators meet the relevant national or industry standards.

[0146] Example 5 Amino Acid Facial Cleanser

[0147] In this embodiment, an amino acid facial cleanser was prepared using composition 2 from Example 1. The raw material composition and formulation are shown in Table 15.

[0148] Table 15 Composition and Proportion of Ingredients in Amino Acid Facial Cleanser

[0149]

[0150] The preparation method is as follows:

[0151] 1. Add each component of phase A to the mixing vessel and heat it to about 85°C with an appropriate stirring speed to ensure the materials are mixed evenly;

[0152] 2. Maintain a temperature of approximately 85°C and add phase B;

[0153] 3. Cool down to about 50℃, add phase C and stir well;

[0154] 4. Keep the temperature at around 40℃, add phase D and stir thoroughly for 2-3 hours. The material will begin to solidify. Reduce the speed and stir slowly until it is completely solidified.

[0155] 5. Once the sample passes inspection, the material can be discharged.

[0156] The amino acid facial cleanser of this embodiment is a white, creamy texture with a soft consistency and a slight pearlescent sheen. It produces fine foam and is easy to rinse. It contains no thickeners other than thickener composition 2. It has no odor other than added fragrance. After being placed in temperature (40℃±2℃) and cold (-5℃±2℃) tests, the product showed no change in color or odor, and exhibited no suspended matter, sediment, or crystallization. All product indicators meet the relevant national or industry standards.

[0157] Example 6 Amino Acid Shampoo

[0158] This embodiment uses composition 3 from Example 1 to prepare an amino acid shampoo.

[0159] Table 16 Composition and Proportion of Amino Acid Shampoo Ingredients

[0160]

[0161]

[0162] The preparation method is as follows:

[0163] 1. Add each component of phase A to the stirred tank, heat to about 50°C, and stir at an appropriate speed;

[0164] 2. After all components in phase A have completely dissolved, cool to 40°C, add all components in phase B, and mix thoroughly.

[0165] 3. After adding phase C, stir thoroughly and stop heating;

[0166] 4. Add phase D and stir well;

[0167] 5. Once the sample passes the test, the material can be discharged.

[0168] This amino acid shampoo is free of silicone oil and sulfates, has a clear and transparent appearance, and contains no thickeners other than thickener composition 3. Its viscosity is approximately 2000 mPa·s. It produces rich lather, has excellent cleaning power, and is easy to rinse. It has no odor other than added fragrance. After temperature (40℃±2℃) and cold (-5℃±2℃) tests, the product showed no change in color or odor, and exhibited no suspended matter, sediment, or crystallization. All product indicators meet the relevant national or industry standards.

[0169] Comparative Example 1

[0170] Commonly used thickeners in amino acid surfactant systems include SF-1 (acrylate copolymer), CMEA (coconut oil fatty acid monoethanolamine), and DOE-120 (PEG-120 methyl glucoside ester). In this example, the thickening effects of the above three thickeners were tested in the same thickener-free matrix formulation and compared with composition 1. The test formulations are shown in Table 17, and the thickening effects are shown in Table 18.

[0171] Table 17 Thickener-free matrix formulations

[0172]

[0173]

[0174] Table 18 Thickening test results of different thickeners on matrix formulations

[0175] Types of thickeners Amount added, wt% Viscosity, MPAs Thickener-free (base formulation) / 336 Composition 1 0.8 1044 Composition 1 1.5 2380 SF-1 0.8 621 SF-1 1.5 1360 CMEA 0.8 703 CMEA 1.5 1241 DOE-120 0.8 601 DOE-120 1.5 1158

[0176] As can be seen from Tables 17 and 18, when the amount of thickener added is the same, the thickening effect of Composition 1 is better than that of SF-1, DOE-120 and CMEA.

[0177] Although the embodiments of this application have been described above in conjunction with the specific embodiments described, this application is not limited to the specific embodiments and application fields described above. The specific embodiments described above are merely illustrative and instructive, and not restrictive. Those skilled in the art can make many other forms based on the teachings of this specification and without departing from the scope of protection of the claims of this application, and these are all within the scope of protection of this application.

Claims

1. A composition having a thickening effect, wherein, The active thickening ingredient comprises 0.0001 wt% to 99.9999 wt% of a biosurfactant, wherein the thickening is for amino acid surfactants, and the biosurfactant is the sole active ingredient for thickening with amino acid surfactants. The biosurfactant is a lipopeptide; The lipopeptide is selected from one or more of surfactants, itursin, and fenbufen; The surfactant was prepared by the following method: Single colonies of Bacillus subtilis THY-7 / Pg3-srfA were picked and inoculated into LB liquid medium to obtain seed culture. Then, 5% of the seed culture was transferred to a shake flask of fermentation medium. After 2-6 hours of culture, 1 Mm IPTG was added and cultured for another 2 days to obtain a fermentation broth containing surfactant. The surfactant was then purified. The amino acid surfactant is selected from one or more of the following types: glycine, sarcosine, glutamic acid, alanine, or taurine. The mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:

50.

2. The composition according to claim 1, wherein, The hydrophilic amino acid in the amino acid surfactant has a molecular weight of less than 150 Da.

3. The composition according to claim 1, wherein, The mass ratio of the biosurfactant to the amino acid surfactant is 1:5 to 1:

30.

4. The use of the composition having a thickening effect according to any one of claims 1-3, wherein the composition having a thickening effect is added as a thickener to a cosmetic.

5. An amino acid surfactant composition, wherein, Includes amino acid surfactants and the thickening composition according to any one of claims 1-3.

6. The composition according to claim 5, wherein, The content of the biosurfactant is 5 wt% to 90 wt% based on the weight of the amino acid surfactant composition.

7. The composition according to claim 6, wherein, The content of the biosurfactant is 10wt% to 50wt% based on the weight of the amino acid surfactant composition.

8. The composition according to claim 5, wherein, It also includes other surfactants besides amino acid surfactants.

9. The composition according to claim 8, wherein, The other surfactants are selected from one or more of anionic surfactants, nonionic surfactants, or amphoteric surfactants.

10. The composition according to claim 5, wherein, The composition further includes one or more of water, inorganic salts, humectants, pH adjusters, and preservatives.

11. The composition according to claim 10, wherein, The inorganic salt is sodium chloride.

12. The composition according to claim 11, wherein, The mass ratio of sodium chloride to amino acid surfactant is 1:10 to 1:

30.

13. The uses of biosurfactants in amino acid surfactants, among which, The biosurfactant acts as a thickener in amino acid surfactants, and is the only active ingredient for thickening amino acid surfactants. The biosurfactant is a lipopeptide; The lipopeptide is selected from one or more of surfactants, itursin, and fenbufen; The surfactant was prepared by the following method: Single colonies of Bacillus subtilis THY-7 / Pg3-srfA were picked and inoculated into LB liquid medium to obtain seed culture. Then, 5% of the seed culture was transferred to a shake flask of fermentation medium. After 2-6 hours of culture, 1 Mm IPTG was added and cultured for another 2 days to obtain a fermentation broth containing surfactant. The surfactant was then purified. The amino acid surfactant is selected from one or more of the following types: glycine, sarcosine, glutamic acid, alanine, or taurine. The mass ratio of the biosurfactant to the amino acid surfactant is 1:1 to 1:

50.

14. The composition according to any one of claims 5-12 or the use according to claim 13, wherein, The hydrophilic amino acid in the amino acid surfactant has a molecular weight of less than 150 Da.

15. The composition according to any one of claims 5-12 or the use according to claim 13, wherein, The mass ratio of the biosurfactant to the amino acid surfactant is 1:5 to 1:30.

Images