A type of mite remover for cosmetics

Abstract

This invention discloses a mite-removing agent for cosmetics, relating to the field of cosmetic additive technology. This invention addresses the technical problem of existing technologies that do not combine multiple mechanisms such as moisture absorption and moisturizing, antibacterial and bactericidal effects to achieve good antibacterial, long-lasting mite removal, and oil control and acne treatment effects, thus expanding the range of applicable cosmetic products. By adding slow-release granules of Sichuan pepper fruit extract and high-molecular-weight wolfberry polysaccharides, this invention addresses the issue. Sichuan pepper fruit extract has a lifting and firming effect on the skin, and its volatile oils and alkaloids have strong bactericidal effects, inhibiting the growth of bacteria, fungi, and mites. When applied to skincare products, it can achieve skin conditioning and anti-aging effects. Wolfberry polysaccharides are easily absorbed by the skin surface and can scavenge free radicals, superoxide anions, and hydroxyl radicals. Therefore, when this mite-removing agent is applied to cosmetic products such as serums, toners, and night creams, it significantly improves the antibacterial, long-lasting mite removal, oil control, and acne treatment effects of cosmetics while remaining natural and harmless.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic additive technology, and specifically relates to a mite remover for cosmetic use. Background Technology

[0002] Mites can parasitize human skin and pose a significant threat to human health. They not only bite and suck blood, damaging the skin, but also cause various diseases such as dermatitis, rashes, internal mite infestations, scrub typhus, scabies, mite-induced allergic asthma, allergic rhinitis, and demodicosis. Existing antibacterial and mite-removing agents often contain toxic chemicals, which are not only unsafe and environmentally friendly, but also seriously affect human health.

[0003] Existing technology (CN113304085A) discloses the application of a plant extract in the preparation of cosmetics with antibacterial and anti-mite functions, belonging to the field of cosmetic technology. The plant extract comprises licorice root extract, eucalyptus leaf extract, Angelica pubescens root extract, Perilla frutescens leaf extract, and water. Cosmetics prepared using this plant extract exhibit strong inhibitory effects against various pathogens and excellent anti-mite effects. Furthermore, the prepared cosmetics are rich in natural products, free of skin-irritating substances, and avoid skin itching, showing promising application prospects. However, due to the complex composition of natural extracts, they cannot combine multiple mechanisms such as hygroscopic moisturizing, antibacterial, and bactericidal effects to achieve good antibacterial, long-lasting anti-mite, oil-controlling, and acne-reducing effects, thus limiting the range of applicable cosmetic products. Summary of the Invention

[0004] The purpose of this invention is to provide a mite-removing agent for cosmetics, which solves the technical problem that existing technologies cannot combine multiple mechanisms such as moisture absorption and moisturizing, antibacterial and bactericidal effects to achieve good antibacterial, long-lasting mite removal, oil control and acne removal effects, thereby expanding the range of applicable cosmetic products.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] This invention provides a cosmetic mite remover, which is obtained by compounding, sterilizing and sealing the following ingredients in parts by weight: 0.5-2 parts butylene glycol, 0.3-1 parts PEG40, 0.1-0.6 parts hydrogenated castor oil, 0.12-0.35 parts dichlorobenzyl alcohol, 1-3 parts slow-release granules of Sichuan pepper fruit extract, 0.6-1.5 parts multi-molecular weight wolfberry polysaccharide, 0.3-0.8 parts pH adjuster and 2-6 parts organic solvent; wherein, the slow-release granules of Sichuan pepper fruit extract are obtained by enzymatic hydrolysis and microwave extraction of the pericarp and seeds of Sichuan pepper fruit, followed by encapsulation with Eucommia ulmoides polysaccharide and corn starch, and extrusion; the multi-molecular weight wolfberry polysaccharide is obtained by water extraction, concentration and freeze-drying of wolfberry, followed by degradation through an oxidation-reduction system.

[0007] As a further improvement of the present invention, the preparation method of the sustained-release granules of Sichuan pepper fruit extract includes the following steps:

[0008] Step 1: Crush and squeeze the pericarp and seeds of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 50-60℃ for 6-8 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 25-30mL / g, microwave extract at 520-550W irradiation power for 30-60min, centrifuge, and remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0009] Step 2: Add Eucommia ulmoides polysaccharide powder and Zanthoxylum bungeanum fruit extract to a beaker at a weight ratio of 1.2-1.5:1, seal, heat in a water bath at 80-90℃ for 30-50 minutes, then add the same amount of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add corn starch, place in a mixer and mix evenly at a speed of 200-300 rpm, then pass through a single screw extruder to extrude and form Zanthoxylum bungeanum fruit extract sustained-release granules.

[0010] As a further improvement of the present invention, the mixed enzymes include pectinase, cellulase, hemicellulase and xylanase, with addition amounts of 0.3-0.4%, 0.5-0.7%, 0.03-0.06% and 1.1-1.3% of the weight of the fine powder of Sichuan pepper fruit, respectively; the addition amount of tea saponin is 3-5% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0011] As a further improvement of the present invention, the amount of corn starch used is 1 to 1.5 times the weight of Eucommia ulmoides polysaccharide powder, the screw speed of the single screw extruder is 300 to 400 rpm, the barrel diameter is 20 mm, and the die diameter is 1 to 2 mm.

[0012] As a further improvement of the present invention, the extraction method of the multi-molecular weight Lycium barbarum polysaccharide includes the following steps:

[0013] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries are crushed and sieved. Deionized water is added at a material-to-liquid ratio of 25-30 mL / g. The temperature is raised to 80-90℃ and the mixture is extracted twice. The mixture is filtered, and the filtrate is centrifuged at 4000-6000 rpm for 8-10 min. The supernatant is concentrated under reduced pressure at 60℃. Ethanol is added at 3-5 times the weight of the concentrate. The mixture is allowed to stand at 5-10℃ for 12 hours. The mixture is then centrifuged at 7000-8000 rpm for 5 min. The supernatant is removed, and the solid precipitate is freeze-dried at -30 to -10℃ to obtain crude wolfberry polysaccharides.

[0014] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 10-20 mg / mL. Vitamin C, 2 wt% hydrogen peroxide and ferrous sulfate heptahydrate were mixed evenly and added to the polysaccharide solution. The mixture was reacted at room temperature in the dark for 2-3 hours. The solution was then placed in a dialysis bag with a capacity of 400-500 Da and dialyzed in pure water for 3 days. The dialysate was centrifuged at 7000-8000 rpm. The supernatant was freeze-dried at -30 to -10℃ to constant weight and then pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0015] As a further improvement of the present invention, the ratio of the polysaccharide solution to vitamin C, hydrogen peroxide, and ferrous sulfate heptahydrate is 30 mL: 0.1 mol: 2 mL: 0.06 mol.

[0016] As a further improvement of the present invention, the pH adjuster is one or a mixture of sodium carbamate, sodium tripolyphosphate, and fruit acid, and the organic solvent is one or a mixture of propylene glycol, butanediol, and diethylene glycol.

[0017] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0018] 1. Compared with the prior art, the cosmetic mite-removing agent of the present invention, in addition to the conventional wetting agent butylene glycol, emulsifier PEG40, thickener hydrogenated castor oil, and preservative dichlorobenzyl alcohol, adds slow-release granules of Sichuan pepper fruit extract and high molecular weight wolfberry polysaccharide. Sichuan pepper fruit extract has the effect of lifting and firming the skin, and its volatile oil and alkaloids have a strong bactericidal effect, inhibiting the growth of bacteria, fungi, and mites, thus improving the mite-removing and antibacterial functions. When applied to skin care products, it can achieve skin conditioning and anti-aging effects. Wolfberry polysaccharide is easily absorbed by the skin surface and can scavenge free radicals, superoxide anions, and hydroxyl radicals. Therefore, when this mite-removing agent is applied to cosmetic products such as serums, toners, and night creams, it significantly improves the antibacterial, long-lasting mite-removing, oil-controlling, and acne-removing effects of cosmetics without being natural and harmless.

[0019] 2. The slow-release granules of Sichuan pepper fruit extract of the present invention, compared with the traditional water extraction, alcohol extraction and microwave extraction methods of Sichuan pepper fruit extract, involve adding mixed enzymes and tea saponin for enzymatic hydrolysis followed by microwave heating extraction to obtain Sichuan pepper fruit extract; the Sichuan pepper fruit extract is then coated with Eucommia ulmoides polysaccharide powder and corn starch and extruded to form spherical slow-release granules with uniform and stable particle size; considering that the pericarp of Sichuan pepper fruit is rich in plant fiber, wrapping and supporting the fruit is not conducive to the release of volatile oils, alkaloids, fatty oils and polyphenolic compounds inside; various biological enzymes can decompose different types of plant fiber into simple monosaccharides or oligosaccharides, and tea saponin, as a natural nonionic surfactant, reduces surface activity; the slow-release granules are biodegradable and have good controlled release, promoting the good and long-lasting antibacterial and anti-mite effect of the acaricide.

[0020] 3. The multi-molecular weight Lycium barbarum polysaccharide of the present invention is obtained by adding a redox system composed of vitamin C, hydrogen peroxide and ferrous sulfate heptahydrate to the Lycium barbarum polysaccharide solution, and then performing dialysis, centrifugation and freeze-drying to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation. This not only improves the antioxidant properties of the polysaccharide, but also enhances its moisture absorption and moisturizing effect when applied to cosmetics, and provides certain protection and repair effects for cells. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 A flowchart illustrating the preparation method of the cosmetic mite remover of the present invention is shown. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] The Sichuan pepper fruit varieties mentioned in this application are mainly selected from Sichuan Hanyuan pepper and Shaanxi Hancheng Dahongpao pepper. These peppers are large, uniform in size, of high quality, and contain high levels of natural active ingredients. Sichuan pepper fruit extract, traditionally understood as a substance extracted from Sichuan pepper, soothes subcutaneous muscle tissue under the action of various ion channels, has a temporary and reversible effect on nerve conduction, affects sensations of heat and touch, and reduces skin discomfort and itching. Sichuan pepper fruit extract is a plant extract with skin-lifting and firming properties, and also has anti-allergic, anti-inflammatory, and antibacterial functions. Its main role in skincare products is skin conditioning and anti-aging, and its product forms are mainly serums, toners, and night creams.

[0025] The main components of Sichuan pepper fruit extract include the following categories:

[0026] 1. Zanthoxylates: The main components are zanthoxylate and citric acid zanthoxylate, which are the main aromatic components of Sichuan pepper, giving it its unique spiciness and aroma;

[0027] 2. Terpenoids: such as β-cineole and α-pinene, these compounds are also among the aroma components of Sichuan pepper;

[0028] 3. Coumarin compounds: such as apigenin, have antioxidant and anti-inflammatory effects;

[0029] 4. Polyphenolic compounds: such as catechins and flavonoids, are natural antioxidants in plants that help protect cells from oxidative stress damage;

[0030] 5. Alkaloids: In addition to xanthoalkaloids, it also contains other alkaloids, such as xanthoalkalone.

[0031] Goji berry polysaccharide is a water-soluble polysaccharide extracted from the goji berry plant. It is one of the main active ingredients in goji berries. It has been identified as a proteoglycan, mainly composed of monosaccharides such as arabinose, glucose, galactose, mannose, xylose, and rhamnose. It has the functions of enhancing immunity and immune regulation, promoting hematopoietic function, lowering blood lipids, anti-tumor effect, and anti-aging effect.

[0032] The main methods for extracting polysaccharides from wolfberry are as follows:

[0033] 1. Water extraction (water immersion method): This is one of the most common methods for extracting polysaccharides from wolfberries. First, wolfberries are soaked in an appropriate amount of water, allowing the polysaccharide components to dissolve from the plant material into the water to form an extract. Then, the extract is separated through a filter or centrifuge to obtain an aqueous solution containing wolfberry polysaccharides. Finally, through concentration and dehydration steps, pure wolfberry polysaccharides are obtained.

[0034] 2. Pure water decoction method: Put goji berries into a pot, add an appropriate amount of water, and bring to a boil over medium heat; after boiling, turn to low heat and simmer for a period of time to allow the polysaccharide components to gradually dissolve into the water; finally, filter out the decoction water to obtain an aqueous solution containing goji berry polysaccharides.

[0035] 3. Ultrasonic-assisted extraction method: Place wolfberries and an appropriate amount of solvent (such as water or ethanol) into an ultrasonic extractor. Use ultrasound to promote the release of polysaccharide components from the plant material into the solvent. Then, separate the extract through centrifugation and other steps to obtain a solution containing wolfberry polysaccharides.

[0036] 4. Ethanol extraction method: Goji berries are placed in an ethanol solution for extraction. Ethanol effectively extracts polysaccharide components. Then, through concentration and evaporation, pure goji berry polysaccharides are obtained.

[0037] Example 1

[0038] See Figure 1 As shown, a cosmetic mite remover of this embodiment is obtained by mixing and compounding the following ingredients by weight, sterilizing by ultraviolet irradiation, and then sealing and packaging: 1.1 kg of butylene glycol, 0.6 kg of PEG40, 0.4 kg of hydrogenated castor oil, 0.26 kg of dichlorobenzyl alcohol, 1.8 kg of slow-release granules of Sichuan pepper fruit extract, 1.3 kg of multi-molecular-weight wolfberry polysaccharide, 0.5 kg of pH adjuster fruit acid, and 3 kg of organic solvent propylene glycol.

[0039] The preparation method of Sichuan pepper fruit extract sustained-release granules includes the following steps:

[0040] Step 1: Crush and squeeze the pericarp and seeds of 1 kg of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 56℃ for 7.5 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 27mL / g, microwave heat extraction at 540W irradiation power for 50min, centrifuge, remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0041] The mixed enzymes include pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.35%, 0.58%, 0.05%, and 1.18% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added is 3.8% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0042] Step 2: Add 140g of Eucommia ulmoides polysaccharide powder and 100g of Zanthoxylum bungeanum fruit extract to a beaker, seal, heat in an 86℃ water bath for 45 minutes, then add another 100g of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add 180g of corn starch, place in a mixer and mix evenly at 290rpm. Pass the mixture into a single-screw extruder and extrude to obtain Zanthoxylum bungeanum fruit extract slow-release granules. The screw speed of the single-screw extruder is 380rpm, the barrel diameter is 20mm, and the die orifice diameter is 1mm.

[0043] The extraction method for high molecular weight Lycium barbarum polysaccharides includes the following steps:

[0044] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries were crushed and sieved. Deionized water was added at a material-to-liquid ratio of 28 mL / g. The temperature was raised to 90℃ and the water was extracted twice. The mixture was filtered, and the filtrate was centrifuged at 5000 rpm for 9 min. The supernatant was concentrated under reduced pressure at 60℃. Ethanol with a weight of 4 times that of the concentrate was added. The mixture was allowed to stand at 8℃ for 12 hours and centrifuged at 7500 rpm for 5 min. The supernatant was removed, and the solid precipitate was freeze-dried at -20℃ to obtain crude wolfberry polysaccharides.

[0045] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 16 mg / mL. 176.12 g of vitamin C, 20 mL of 2 VT% hydrogen peroxide, and 166.8 g of ferrous sulfate heptahydrate were mixed evenly and added to 300 mL of the polysaccharide solution. The mixture was reacted at room temperature in the dark for 2.5 hours. The solution was then placed in a 450 Da dialysis bag and dialyzed in pure water for 3 days. The dialysate was centrifuged at 7500 rpm, and the supernatant was freeze-dried at -22℃ to constant weight. The supernatant was then pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0046] Example 2

[0047] See Figure 1 As shown, a cosmetic mite remover of this embodiment is obtained by mixing and compounding the following ingredients by weight, sterilizing by ultraviolet irradiation, and then sealing and packaging: 0.7 kg of butylene glycol, 0.4 kg of PEG40, 0.4 kg of hydrogenated castor oil, 0.28 kg of dichlorobenzyl alcohol, 2.5 kg of slow-release granules of Sichuan pepper fruit extract, 1.2 kg of multi-molecular-weight wolfberry polysaccharide, 0.7 kg of sodium tripolyphosphate pH adjuster, and 5 kg of diethylene glycol organic solvent.

[0048] The preparation method of Sichuan pepper fruit extract sustained-release granules includes the following steps:

[0049] Step 1: Crush and squeeze the pericarp and seeds of 1 kg of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 60℃ for 6.5 hours for enzymatic hydrolysis, filter under reduced pressure, and wash the filter cake with a pH=5 buffer solution. Add water to the filter residue to make the material-to-liquid ratio reach 30 mL / g, microwave extract at 550W irradiation power for 40 min, centrifuge, and remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0050] The mixed enzymes include pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.38%, 0.63%, 0.055%, and 1.3% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added is 4.8% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0051] Step 2: Add 130g of Eucommia ulmoides polysaccharide powder and 100g of Zanthoxylum bungeanum fruit extract to a beaker, seal, heat in a 90℃ water bath for 45 minutes, then add another 100g of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add 182g of corn starch, place in a mixer and mix evenly at 260 rpm. Pass the mixture into a single-screw extruder and extrude to obtain Zanthoxylum bungeanum fruit extract slow-release granules. The screw speed of the single-screw extruder is 400 rpm, the barrel diameter is 20 mm, and the die orifice diameter is 1.2 mm.

[0052] The extraction method for high molecular weight Lycium barbarum polysaccharides includes the following steps:

[0053] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries were crushed and sieved. Deionized water was added at a material-to-liquid ratio of 27 mL / g. The temperature was raised to 90℃ and the water was extracted twice. The mixture was filtered, and the filtrate was centrifuged at 5300 rpm for 9 min. The supernatant was concentrated under reduced pressure at 60℃. Ethanol of 4.5 times the weight of the concentrate was added, and the mixture was allowed to stand at 8℃ for 12 hours. The mixture was then centrifuged at 7800 rpm for 5 min. The supernatant was removed, and the solid precipitate was freeze-dried at -15℃ to obtain crude wolfberry polysaccharides.

[0054] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 16 mg / mL. 176.12 g of vitamin C, 20 mL of 2 VT% hydrogen peroxide and 166.8 g of ferrous sulfate heptahydrate were mixed evenly and added to 300 mL of the polysaccharide solution. The mixture was reacted at room temperature in the dark for 3 hours. The solution was then placed in a 500 Da dialysis bag and dialyzed in pure water for 3 days. The dialysate was centrifuged at 8000 rpm. The supernatant was freeze-dried at -22℃ to constant weight and pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0055] Example 3

[0056] See Figure 1 As shown, a cosmetic mite remover of this embodiment is obtained by mixing and compounding the following ingredients by weight, sterilizing by ultraviolet irradiation, and then sealing and packaging: 1.8 kg of butylene glycol, 0.9 kg of PEG40, 0.2 kg of hydrogenated castor oil, 0.16 kg of dichlorobenzyl alcohol, 2.8 kg of slow-release granules of Sichuan pepper fruit extract, 1.4 kg of multi-molecular-weight wolfberry polysaccharide, 0.7 kg of sodium carbamate (pH adjuster), and 2.5 kg of butylene glycol (organic solvent).

[0057] The preparation method of Sichuan pepper fruit extract sustained-release granules includes the following steps:

[0058] Step 1: Crush and squeeze the pericarp and seeds of 1 kg of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 53℃ for 8 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 27mL / g, microwave heat extraction at 535W irradiation power for 50min, centrifuge, remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0059] The mixed enzymes include pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.4%, 0.53%, 0.06%, and 1.3% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added is 3.7% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0060] Step 2: Add 150g of Eucommia ulmoides polysaccharide powder and 100g of Zanthoxylum bungeanum fruit extract to a beaker, seal, heat in an 88℃ water bath for 35 minutes, then add another 100g of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add 195g of corn starch, place in a mixer and mix evenly at 300rpm. Pass the mixture into a single-screw extruder and extrude to obtain Zanthoxylum bungeanum fruit extract slow-release granules. The screw speed of the single-screw extruder is 340rpm, the barrel diameter is 20mm, and the die orifice diameter is 1.6mm.

[0061] The extraction method for high molecular weight Lycium barbarum polysaccharides includes the following steps:

[0062] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries were crushed and sieved. Deionized water was added at a material-to-liquid ratio of 28 mL / g. The temperature was raised to 87℃ and the water was extracted twice. The mixture was filtered, and the filtrate was centrifuged at 5200 rpm for 10 min. The supernatant was concentrated under reduced pressure at 60℃. Ethanol was added at 3.8 times the weight of the concentrate. The mixture was allowed to stand at 9℃ for 12 hours. The mixture was centrifuged at 7200 rpm for 5 min. The supernatant was removed, and the solid precipitate was freeze-dried at -25℃ to obtain crude wolfberry polysaccharides.

[0063] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 16 mg / mL. 176.12 g of vitamin C, 20 mL of 2 VT% hydrogen peroxide and 166.8 g of ferrous sulfate heptahydrate were mixed evenly and added to 300 mL of the polysaccharide solution. The mixture was reacted at room temperature in the dark for 3 hours. The solution was then placed in a 500 Da dialysis bag and dialyzed in pure water for 3 days. The dialysate was centrifuged at 8000 rpm. The supernatant was freeze-dried at -25℃ to constant weight and pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0064] Example 4

[0065] See Figure 1 As shown, a cosmetic mite remover of this embodiment is obtained by mixing and compounding the following ingredients by weight, sterilizing by ultraviolet irradiation, and then sealing and packaging: 2 kg of butylene glycol, 1 kg of PEG40, 0.6 kg of hydrogenated castor oil, 0.33 kg of dichlorobenzyl alcohol, 3 kg of slow-release granules of Sichuan pepper fruit extract, 1.4 kg of multi-molecular-weight wolfberry polysaccharide, 0.7 kg of pH adjuster fruit acid, and 5.6 kg of organic solvent propylene glycol.

[0066] The preparation method of Sichuan pepper fruit extract sustained-release granules includes the following steps:

[0067] Step 1: Crush and squeeze the pericarp and seeds of 1 kg of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 52℃ for 6.5 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 27mL / g, microwave heat extraction at 550W irradiation power for 45min, centrifuge, remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0068] The mixed enzymes include pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.34%, 0.58%, 0.053%, and 1.26% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added is 3.7% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0069] Step 2: Add 130g of Eucommia ulmoides polysaccharide powder and 100g of Zanthoxylum bungeanum fruit extract to a beaker, seal, heat in an 85℃ water bath for 45 minutes, then add another 100g of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add 169g of corn starch, place in a mixer and mix evenly at 270 rpm. Pass the mixture into a single-screw extruder and extrude to obtain Zanthoxylum bungeanum fruit extract slow-release granules. The screw speed of the single-screw extruder is 380 rpm, the barrel diameter is 20 mm, and the die orifice diameter is 1.6 mm.

[0070] The extraction method for high molecular weight Lycium barbarum polysaccharides includes the following steps:

[0071] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries were crushed and sieved. Deionized water was added at a material-to-liquid ratio of 30 mL / g. The temperature was raised to 85℃ and the water was extracted twice. The mixture was filtered, and the filtrate was centrifuged at 5500 rpm for 10 min. The supernatant was concentrated under reduced pressure at 60℃. Ethanol was added at 5 times the weight of the concentrate. The mixture was allowed to stand at 8℃ for 12 hours. The mixture was centrifuged at 7600 rpm for 5 min. The supernatant was removed, and the solid precipitate was freeze-dried at -20℃ to obtain crude wolfberry polysaccharides.

[0072] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 16 mg / mL. 176.12 g of vitamin C, 20 mL of 2 VT% hydrogen peroxide, and 166.8 g of ferrous sulfate heptahydrate were mixed evenly and added to 300 mL of the polysaccharide solution. The mixture was reacted at room temperature in the dark for 2.6 hours. The solution was then placed in a 480 Da dialysis bag and dialyzed in pure water for 3 days. The dialysate was centrifuged at 7700 rpm, and the supernatant was freeze-dried at -23℃ to constant weight. The supernatant was then pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0073] Example 5

[0074] See Figure 1 As shown, a cosmetic mite remover of this embodiment is obtained by mixing and compounding the following ingredients by weight, sterilizing by ultraviolet irradiation, and then sealing and packaging: 1.8 kg of butylene glycol, 0.9 kg of PEG40, 0.3 kg of hydrogenated castor oil, 0.32 kg of dichlorobenzyl alcohol, 2.6 kg of slow-release granules of Sichuan pepper fruit extract, 1.4 kg of multi-molecular weight wolfberry polysaccharide, 0.7 kg of pH adjuster, and 3.9 kg of organic solvent; the pH adjuster is obtained by mixing sodium carbamate and fruit acid in a weight ratio of 1:1, and the organic solvent is obtained by mixing propylene glycol and diethylene glycol in a volume ratio of 1:1.

[0075] The preparation method of Sichuan pepper fruit extract sustained-release granules includes the following steps:

[0076] Step 1: Crush and squeeze the pericarp and seeds of 1 kg of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 60℃ for 8 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 28mL / g, microwave heat extraction at 530W irradiation power for 52min, centrifuge, remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract;

[0077] The mixed enzymes included pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.4%, 0.63%, 0.037%, and 1.26% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added was 4.8% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 was prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

[0078] Step 2: Add 126g of Eucommia ulmoides polysaccharide powder and 100g of Zanthoxylum bungeanum fruit extract to a beaker, seal, heat in an 87℃ water bath for 46 minutes, then add another 100g of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add 176g of corn starch, place in a mixer and mix evenly at 270rpm. Pass the mixture into a single-screw extruder and extrude to obtain Zanthoxylum bungeanum fruit extract slow-release granules. The screw speed of the single-screw extruder is 400rpm, the barrel diameter is 20mm, and the die orifice diameter is 2mm.

[0079] The extraction method for high molecular weight Lycium barbarum polysaccharides includes the following steps:

[0080] Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries were crushed and sieved. Deionized water was added at a material-to-liquid ratio of 25 mL / g. The temperature was raised to 85℃ and the water was extracted twice. The mixture was filtered, and the filtrate was centrifuged at 6000 rpm for 9 min. The supernatant was concentrated under reduced pressure at 60℃. Ethanol with a weight of 5 times that of the concentrate was added. The mixture was allowed to stand at 10℃ for 12 hours. The mixture was centrifuged at 7600 rpm for 5 min. The supernatant was removed, and the solid precipitate was freeze-dried at -25℃ to obtain crude wolfberry polysaccharides.

[0081] Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 16 mg / mL. 176.12 g of vitamin C, 20 mL of 2 VT% hydrogen peroxide and 166.8 g of ferrous sulfate heptahydrate were mixed evenly and added to 300 mL of the polysaccharide solution. The mixture was reacted at room temperature in the dark for 3 hours. The solution was then placed in a 500 Da dialysis bag and dialyzed in pure water for 3 days. The dialysate was centrifuged at 7900 rpm. The supernatant was freeze-dried at -26℃ to constant weight and pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

[0082] Compare with Example 1

[0083] The difference between this comparative example of a mite remover for cosmetic use and Example 1 is that the slow-release granules of Sichuan pepper fruit extract are replaced with Sichuan pepper fruit extract.

[0084] Compare with Example 2

[0085] The difference between this comparative example of acaricide for cosmetic use and Example 1 is that it does not contain multi-molecular-weight Lycium barbarum polysaccharide.

[0086] Compare with Example 3

[0087] The difference between the cosmetic mite remover in this comparative example and Example 1 is that the multi-molecular weight Lycium barbarum polysaccharide is replaced with crude Lycium barbarum polysaccharide.

[0088] Effect verification experiment

[0089] mite removal effect experiment

[0090] (1) Mite acquisition: Subjects with Demodex brevis parasitizing their facial skin were selected. After washing their face before bed, transparent tape with a length of 5cm and a width of 2cm was applied to multiple locations on the forehead, cheeks, and nose. The tape was removed the next morning and applied to another tape. Demodex brevis was then obtained by microscopic examination.

[0091] (2) Group Experiment: Using water as a solvent, the cosmetic mite-repellents prepared in Examples 1-5 and Control Examples 1-3 were diluted to a concentration of 20 vt%, and a blank control group was set up. 200 μL of the experimental solution was added to each glass slide, spread evenly, and allowed to fully contact the Demodex mites. The slides were placed at a temperature of 25 ± 2℃ and a relative humidity of 70%. After 2 hours, the slides were removed and observed continuously under a microscope for 5 minutes. Killing was determined by the immobility of the mites' claws or legs. The killing status of the Demodex mites was recorded using the microscope's counting function, and the killing rate was calculated. The killing rate was also tested under the same conditions after 3 days and 7 days of standing indoors. The results of the mite-repellent effect experiment are shown in Table 1.

[0092] Table 1. Results of mite-removal efficacy experiment

[0093]

[0094]

[0095] Based on the above experimental results on mite removal effects, the following conclusions can be drawn: 1) The mite remover prepared in the embodiments of the present invention has a good and long-lasting killing effect on Demodex brevis compared with the control example, indicating that the mite removal effect is more superior and longer-lasting; 2) The long-lasting killing effect of control examples 1-3 was reduced due to the deletion and replacement of the slow-release granules of Sichuan pepper fruit extract and the multi-molecular weight Lycium barbarum polysaccharide.

[0096] Antibacterial effect experiment

[0097] According to the "Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products" (QB / T 2738-2012, Industry Standard of the People's Republic of China), the antibacterial rate of 20 vt% experimental solutions from Examples 1-5 and Comparative Examples 1-3 was tested. The tested bacteria was Malassezia, and the test time was 20 min. The results of the antibacterial effect experiment are shown in Table 2.

[0098] Table 2. Results of antibacterial effect experiment

[0099]

[0100]

[0101] Observing the above-mentioned antibacterial effect test results, it can be found that the mite remover of the present invention has a good antibacterial effect.

[0102] Oil control and acne treatment experiment

[0103] Twenty subjects with excessive facial oil and frequent acne were selected. Oil control and acne treatment tests were conducted using 20 VT% experimental solutions from Examples 1-5 and Comparative Examples 1-3. 10 mL was applied each time and massaged until absorbed. The duration of oil control was determined by whether the face felt clean and non-greasy to the touch. The acne-reducing effect was also observed. The results showed that 80% of the subjects experienced effective oil control for 2-3 days, and 70% of the subjects showed significant signs of fading acne scars and pits.

[0104] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

[0105] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to specific implementations. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A mite-removing agent for cosmetic use, characterized in that, The product is prepared by compounding, sterilizing, and sealing the following components in parts by weight: 0.5-2 parts butylene glycol, 0.3-1 parts PEG40, 0.1-0.6 parts hydrogenated castor oil, 0.12-0.35 parts dichlorobenzyl alcohol, 1-3 parts slow-release granules of Sichuan pepper fruit extract, 0.6-1.5 parts multi-molecular-weight wolfberry polysaccharide, 0.3-0.8 parts pH adjuster, and 2-6 parts organic solvent. The slow-release granules of Sichuan pepper fruit extract are obtained by enzymatic hydrolysis and microwave extraction of the pericarp and seeds of Sichuan pepper fruit, followed by encapsulation with Eucommia ulmoides polysaccharide and corn starch, and then extrusion. The multi-molecular-weight wolfberry polysaccharide is obtained by water extraction, concentration, freeze-drying, and degradation via an oxidation-reduction system from wolfberry. The preparation method of the sustained-release granules of Sichuan pepper fruit extract includes the following steps: Step 1: Crush and squeeze the pericarp and seeds of Sichuan pepper fruit, pass through a 10-mesh sieve to obtain fine Sichuan pepper fruit powder, add it to a round-bottom flask, add mixed enzymes and tea saponins, incubate at 50~60℃ for 6~8 hours for enzymatic hydrolysis, filter under reduced pressure, wash the filter cake with pH=5 buffer solution, add water to the filter residue to make the material-to-liquid ratio reach 25~30mL / g, microwave heat extraction at 520~550W irradiation power for 30~60min, centrifuge, remove the aqueous phase by rotary evaporation to obtain Sichuan pepper fruit extract; Step 2: Add Eucommia ulmoides polysaccharide powder and Zanthoxylum bungeanum fruit extract to a beaker at a weight ratio of 1.2~1.5:1, seal, heat in a water bath at 80~90℃ for 30~50 minutes, then add the same amount of Zanthoxylum bungeanum fruit extract. After the reaction system is completely melted, add corn starch, place in a mixer and mix evenly at a speed of 200~300 rpm, then pass through a single screw extruder to extrude and form Zanthoxylum bungeanum fruit extract sustained-release granules. The extraction method of the multi-molecular-weight Lycium barbarum polysaccharide includes the following steps: Extraction of crude polysaccharides from wolfberry: Washed and drained wolfberries are crushed and sieved. Deionized water is added at a material-to-liquid ratio of 25-30 mL / g. The temperature is raised to 80-90℃ and the mixture is extracted twice. The mixture is filtered, and the filtrate is centrifuged at 4000-6000 rpm for 8-10 min. The supernatant is concentrated under reduced pressure at 60℃. Ethanol is added at 3-5 times the weight of the concentrate. The mixture is allowed to stand at 5-10℃ for 12 hours. The mixture is then centrifuged at 7000-8000 rpm for 5 min. The supernatant is removed, and the solid precipitate is freeze-dried at -30 to -10℃ to obtain crude wolfberry polysaccharides. Extraction of multi-molecular weight Lycium barbarum polysaccharides: Crude Lycium barbarum polysaccharides were dissolved in water to form a polysaccharide solution with a concentration of 10-20 mg / mL. Vitamin C, 2 wt% hydrogen peroxide and ferrous sulfate heptahydrate were mixed evenly and added to the polysaccharide solution. The mixture was reacted at room temperature in the dark for 2-3 hours. The solution was then placed in a dialysis bag with a capacity of 400-500 Da and dialyzed in pure water for 3 days. The dialysate was centrifuged at 7000-8000 rpm. The supernatant was freeze-dried at -30 to -10℃ to constant weight and then pulverized to obtain multi-molecular weight Lycium barbarum polysaccharides with different degrees of degradation.

2. The cosmetic mite remover according to claim 1, characterized in that, The mixed enzymes include pectinase, cellulase, hemicellulase, and xylanase, added at amounts of 0.3-0.4%, 0.5-0.7%, 0.03-0.06%, and 1.1-1.3% of the weight of the fine powder of Sichuan pepper fruit, respectively; the amount of tea saponin added is 3-5% of the weight of the fine powder of Sichuan pepper fruit; the buffer solution with pH=5 is prepared by mixing 0.2 mol / L sodium dihydrogen phosphate solution and sodium hydroxide solution.

3. The acaricide for cosmetic use according to claim 1, characterized in that, The amount of corn starch used is 1 to 1.5 times the weight of the Eucommia ulmoides polysaccharide powder. The screw speed of the single screw extruder is 300 to 400 rpm, the barrel diameter is 20 mm, and the die diameter is 1 to 2 mm.

4. The acaricide for cosmetic use according to claim 1, characterized in that, The ratio of the polysaccharide solution to vitamin C, hydrogen peroxide, and ferrous sulfate heptahydrate is 30 mL: 0.1 mol: 2 mL: 0.06 mol.

5. The acaricide for cosmetic use according to claim 1, characterized in that, The pH adjuster is one or a mixture of sodium carbamate, sodium tripolyphosphate, and fruit acid, and the organic solvent is one or a mixture of propylene glycol, butanediol, and diethylene glycol.

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