Photoelectric synergistic application device and method

Abstract

The present disclosure provides a photoelectric synergistic application device and method, and relates to the technical field of medical devices. The photoelectric synergistic application device comprises: a conductive part with a cavity, used for applying an electric pulse to a target area; and a light irradiation part arranged in the cavity of the conductive part, used for applying light irradiation to the target area; wherein the light irradiation part comprises a light transmission unit and at least one light output unit connected to the light transmission unit, and the at least one light output unit is used for outputting light of a specific wavelength conducted in the light transmission unit. In this way, the penetration of a photosensitizer to a cell membrane can be improved.

Description

Technical Field

[0001] This disclosure relates to the field of medical device technology, specifically to a photoelectric synergistic application device and method. Background Technology

[0002] Photodynamic therapy (PDT) is a novel method for treating diseases such as tumors and proliferative skin diseases using photosensitizing drugs and light activation. In this technique, a photosensitizer is first injected intravenously, applied topically, or orally into the cell membrane of cells in the target area. Then, light of a specific wavelength is irradiated onto the photosensitizer, activating it and producing a photosensitizing effect that kills cells in the target area.

[0003] However, due to the properties of the photosensitizer itself (such as molecular size, charge, hydrophilicity, etc.) and the obstruction of blood vessels and other normal tissues, the penetration of the photosensitizer into cells in the target area is affected, and the therapeutic effect is correspondingly weakened. Summary of the Invention

[0004] In view of this, the present disclosure provides a photoelectric synergistic application device and method to solve the problem of low cell penetration of photosensitizers in related technologies.

[0005] In a first aspect, a photoelectric co-application device is provided, comprising: a conductive part having a cavity for applying an electrical pulse to a target area; and an illumination part disposed in the cavity of the conductive part for applying illumination to the target area; wherein the illumination part includes a light transmission unit and at least one light output unit connected to the light transmission unit, the at least one light output unit being used to output the light transmitted in the light transmission unit.

[0006] In some embodiments, the conductive portion includes a first conductive unit having a cavity, and at least one optical output unit includes a first optical output unit disposed at the end of the cavity of the first conductive unit.

[0007] In some embodiments, the conductive portion further includes: a second conductive unit disposed around the periphery of the first conductive unit; and an insulating unit disposed between the first conductive unit and the second conductive unit for electrically isolating the first conductive unit and the second conductive unit; wherein the first conductive unit is used to apply a first polarity electrical pulse to the target area, and the second conductive unit is used to apply a second polarity electrical pulse to the target area, the polarities of the first polarity electrical pulse and the second polarity electrical pulse being opposite.

[0008] In some embodiments, the first conductive unit includes a first portion and a second portion connected axially, the outer diameter of the first portion being larger than the outer diameter of the second portion; the insulating unit includes a first insulating portion and a second insulating portion, the first insulating portion and the second insulating portion being sleeved on the periphery of the second portion, and the second conductive unit being sleeved on the periphery of the second insulating portion, wherein, in the axial direction of the first conductive unit, the first insulating portion is disposed between the first portion and the second conductive unit.

[0009] In some embodiments, the first conductive unit is provided with a tip having an inclined cut at one end of the first light output unit on the first light output unit side.

[0010] In some embodiments, the conductive portion further includes at least one radial channel formed on the sidewall of the conductive portion, each radial channel accommodating a light output unit.

[0011] In some embodiments, there are multiple radial channels, which are located on the same cross section of the conductive part, and the cross section is perpendicular to the radial direction of the conductive part.

[0012] In some embodiments, the included angle between any two adjacent radial channels is the same.

[0013] In some embodiments, both the end of the cavity of the conductive part and the end of the radial channel are provided with transparent seals.

[0014] In some embodiments, the photoelectric co-application device further includes a blocking plate for illuminating the target area with light emitted from at least one light output unit.

[0015] In a second aspect, a photoelectric co-application device is provided, comprising: the photoelectric co-application device provided in the first aspect; a light generating unit connected to a light illuminating unit in the photoelectric co-application device for generating light; and an electrical pulse generating unit connected to a conductive unit in the photoelectric co-application device for generating electrical pulses.

[0016] Thirdly, a photoelectric co-application method is provided, applied to the photoelectric co-application device provided in the first aspect above. The method includes: applying an electrical pulse to a target region using the photoelectric co-application device to generate micropores on the cell membrane of the target region; and outputting light of a specific wavelength to the target region using the photoelectric co-application device so that a photosensitizer in the target region combines with the light of the specific wavelength and becomes excited.

[0017] In some embodiments, applying an electrical pulse to a target region using a photoelectric co-application device includes applying a reversible electroporation pulse to the target region using the photoelectric co-application device, the reversible electroporation pulse being used to generate reversible micropores on the cell membrane of the target region.

[0018] In some embodiments, after applying a reversible electroporation pulse to a target region using a photoelectric co-application device, the method further includes: applying an irreversible electroporation pulse to the target region using the photoelectric co-application device, the irreversible electroporation pulse being used to generate irreversible micropores on the cell membrane of the target region.

[0019] In some embodiments, applying an electrical pulse to a target area using a photoelectric co-application device includes applying an irreversible electroporation electrical pulse to the target area using the photoelectric co-application device.

[0020] In some embodiments, the irradiation time of light of a specific wavelength includes at least one of the following: before the application of the electrical pulse, during the application of the electrical pulse, synchronously with the application of the electrical pulse, and after the application of the electrical pulse.

[0021] The photoelectric co-application device disclosed herein can apply an electrical pulse to a target area via a conductive part. The electrical pulse causes perforation of the cell membrane of cells in the target area, thereby allowing photosensitizers free outside the cells to enter the cell through micropores in the cell membrane. This enhances the permeability of the photosensitizer to the cell membrane. Attached Figure Description

[0022] Figure 1 The diagram shown is a structural schematic of a photoelectric co-application device provided in an embodiment of this disclosure.

[0023] Figure 2 The diagram shown is a structural schematic of another photoelectric co-application device provided in an embodiment of this disclosure.

[0024] Figure 3 The diagram shown is a structural schematic of another photoelectric co-application device provided in an embodiment of this disclosure.

[0025] Figure 4 As shown Figure 3 A cross-sectional schematic diagram along the direction of the dotted line in the embodiment.

[0026] Figure 5 The diagram shown is a schematic flowchart of a photoelectric synergistic application method provided in an embodiment of this disclosure.

[0027] Figure 6 The diagram shown is a schematic flowchart of another photoelectric synergistic application method provided in an embodiment of this disclosure.

[0028] Figure 7 The diagram shown is a flowchart illustrating another photoelectric synergistic application method provided in this embodiment.

[0029] Figure label:

[0030] 110 - Conductive part; 111 - First conductive unit; 1111 - First part; 1112 - Second part; 112 - Second conductive unit; 113 - Insulating unit; 1131 - First insulating part; 1132 - Second insulating part; 114 - Radial channel; 120 - Illuminating part; 121 - Light transmission unit; 122 - Light output unit. Detailed Implementation

[0031] The technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this disclosure, and not all embodiments. Based on the embodiments of this disclosure, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this disclosure.

[0032] As mentioned above, in photodynamic therapy, the photosensitizer reacts with oxygen under light excitation to generate highly reactive reactive oxygen species (ROS). Excessive ROS within cells can oxidize and damage cellular components such as proteins, nucleic acids, and lipids, inducing apoptosis or necrosis. Therefore, the therapeutic effect of photodynamic therapy depends to some extent on the concentration of the photosensitizer in the cells.

[0033] Due to the protective effect of the cell membrane on the cell, as well as the influence of factors such as the molecular size, charge, and hydrophilicity of the photosensitizer, the photosensitizer is not easy to penetrate the cell membrane and enter the cell, thus reducing the permeability of the photosensitizer and relatively weakening the therapeutic effect.

[0034] In view of this, the present disclosure provides a photoelectric synergistic application device, comprising: a conductive part having a cavity for applying electrical pulses to a target area; and a light irradiation part disposed in the cavity of the conductive part for applying light to the target area; wherein the light irradiation part includes a light transmission unit and at least one light output unit connected to the light transmission unit, the at least one light output unit being used to output light of a specific wavelength transmitted in the light transmission unit. During use, the electrical pulses applied by the device perforate the cell membrane, allowing the photosensitizer to enter the cell through the perforation, increasing the photosensitizer's permeability. Consequently, upon light irradiation, more photosensitizers inside the cells undergo photosensitization, enhancing the therapeutic effect.

[0035] The photoelectric co-application device provided in this disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0036] Figure 1 The image shown is an example of a photoelectric collaborative application device provided in this disclosure, such as... Figure 1 As shown, the photoelectric co-application device includes a conductive part 110 and a light-illuminating part 120.

[0037] The conductive part 110 has an internal cavity, is tubular in shape, and is made of conductive material. The conductive part 110 is connected to an external electrical pulse generating device, enabling the application of electrical pulses generated by the device to the target area. The illumination part 120 is disposed within the cavity of the conductive part 110. The illumination part 120 is connected to an external light generating device, enabling the application of light of a specific wavelength generated by the light generating device to the target area; wherein the wavelength of the light generated by the light generating device should match the type of photosensitizer, so that the photosensitizer inside the cell is excited by the light and causes damage to the cell. The target area is the area where the user wishes to apply light and / or electrical pulses. For example, the target area may be the location of tumor cells or diseased organs.

[0038] Specifically, the illumination unit 120 includes a light transmission unit 121 and a light output unit 122. The light transmission unit 121 can be a light transmission tool such as an optical fiber; one end of the light transmission unit 121 is connected to the light generating device, and the other end is connected to the light output unit 122, so as to transmit light of a specific wavelength generated by the electrical pulse generating device to the light output unit 122. The light output unit 122 further outputs the light of the specific wavelength, so as to illuminate the target area.

[0039] The device in this embodiment applies an electrical pulse to a target area via a conductive part. The electrical pulse causes perforation of the cell membrane in the target area, allowing photosensitizers outside the cell to enter the cell through micropores in the cell membrane. This enhances the permeability of the photosensitizer to the cell membrane. A light-emitting part applies light of a specific wavelength to the target area, exciting the photosensitizers inside the cell and causing damage. Because the concentration of photosensitizers inside the cell increases, the destructive effect is correspondingly enhanced. Therefore, when using the device of this embodiment for photodynamic therapy, the therapeutic effect is significantly improved.

[0040] The specific implementation scheme of the conductive part in the photoelectric co-application device will be described in detail below with reference to the accompanying drawings.

[0041] Figure 2 The image shown is another photoelectric collaborative application device provided in an embodiment of this disclosure. For example... Figure 2 As shown, the conductive part 110 includes a first conductive unit 111 having a cavity. The cavity inside the first conductive unit 111 extends through one end, forming an opening at the end. A light output unit, i.e., a first light output unit, is disposed on one side of the opening of the first conductive unit 111.

[0042] The first light output unit is hemispherical. The bottom surface of the first light output unit is connected to the light transmission unit; the spherical side of the first light output unit faces the opening of the first conductive unit 111. Light of a specific wavelength transmitted in the light transmission unit is directed from the bottom of the first light output unit to the hemispherical surface, refracted on the hemispherical surface, and finally output to the outside of the device through the opening at one end of the first conductive unit 111; more specifically, output to the target area.

[0043] The device in this embodiment is based on the refraction of light by a hemispherical lens, so that light of a specific wavelength is emitted from the first light output unit in all directions, thereby expanding the illumination application area of ​​the device.

[0044] In some embodiments, the first conductive unit 111 has a side with the first light output unit, the end of which is a pointed tip with an inclined cut. This arrangement facilitates puncture of skin tissue to the lesion in the target area.

[0045] In some embodiments, continue to refer to Figure 2 As shown, the conductive part 110 also includes a second conductive unit 112 and an insulating unit 113. The second conductive unit 112 is tubular in shape and is sleeved around the first conductive unit 111. The insulating unit 113 is disposed between the first conductive unit 111 and the second conductive unit 112; one side surface of the insulating unit 113 contacts the outer surface of the first conductive unit 111, and the other side surface of the insulating unit 113 contacts the inner surface of the second conductive unit 112.

[0046] For example, the insulating unit 113 may be a tubular structure made of insulating material, sleeved around the first conductive unit 111; the second conductive unit 112 may be sleeved around the insulating unit 113. Alternatively, the insulating unit 113 may be an insulating coating with insulating effect, the insulating coating being directly coated on the outer surface of the first conductive unit 111, and the second conductive unit 112 being sleeved on the insulating coating.

[0047] When an electrical pulse is applied to the device, the first conductive unit 111 can apply a first polarity electrical pulse, the second conductive unit 112 can apply a second polarity electrical pulse, or the second conductive unit 112 can be grounded.

[0048] When a first polarity electrical pulse is applied to the first conductive unit 111 and the second conductive unit 112 is grounded, the voltage of the second conductive unit 112 is 0V.

[0049] When a first polarity electrical pulse is applied to the first conductive unit 111 and a second polarity electrical pulse is applied to the second conductive unit 112, the polarities of the first and second polarity electrical pulses are opposite. For example, the first polarity electrical pulse is a positive polarity electrical pulse and the second polarity electrical pulse is a negative polarity electrical pulse; or, the first polarity electrical pulse is a negative polarity electrical pulse and the second polarity electrical pulse is a positive polarity electrical pulse. During this process, the insulating unit 113 can electrically isolate the first conductive unit 111 from the second conductive unit 112 to avoid a short circuit.

[0050] Both of the above-described configurations allow for the formation of two electrical pulse application regions on one side of the conductive portion, creating a potential difference between these regions. This generates an electric field zone between the two pulse application regions, disrupting the cell membranes of tissue cells within its coverage area and forming micropores. Furthermore, when two electrical pulses of opposite polarities are applied simultaneously, the generated electric field strength is the sum of the amplitudes of the two pulses. This reduces the device's power supply voltage requirements and correspondingly lowers the requirements for the high voltage resistance and insulation of the components within the device, thus reducing the overall cost of the device.

[0051] For devices that can only apply unipolar pulses, two devices need to be placed in the target area simultaneously; one device applies a first polarity electrical pulse, and the other device applies a second polarity electrical pulse or is grounded, in order to achieve the effect of this embodiment.

[0052] It is understood that in other embodiments, the first conductive unit 111 may be grounded, and a first polarity electrical pulse or a second polarity electrical pulse may be applied to the second conductive unit 112. The goal is simply to create a potential difference between the two conductive units.

[0053] In some embodiments, the first conductive unit 111 includes an axially connected first portion 1111 and a second portion 1112. (Continue referring to...) Figure 2 As shown, the outer diameter of the first part 1111 is larger than the outer diameter of the second part 1112. The insulating unit 113 is sleeved on the periphery of the second part 1112. By setting the thickness of the insulating unit 113, the sum of the thickness of the insulating unit 113 and the outer diameter of the second part 1112 is equal to the outer diameter of the first part 1111, so that the outer surface of the device is smooth overall.

[0054] The insulating unit 113 includes a first insulating portion 1131 and a second insulating portion 1132. As described above, both the first insulating portion 1131 and the second insulating portion 1132 are sleeved around the second portion 1112; further, the second conductive unit 112 is sleeved around the second insulating portion 1132. The first insulating portion 1131 and the second insulating portion 1132 can be tubular structures integrally formed from insulating material; or, the first insulating portion 1131 can be sleeved around the second insulating portion 1132 like the second conductive unit 112, and this disclosure does not impose specific limitations in this regard.

[0055] By setting the thicknesses of the first insulating portion 1131 and the second insulating portion 1132 respectively, the overall outer diameter of the device is made consistent and the outer surface is smooth. For example, the outer diameter of the second conductive unit 112 can be set to be equal to the outer diameter of the first portion 1111, and the second insulating portion 1132 can be uniformly filled between the second conductive unit 112 and the second portion 1112.

[0056] In the axial direction of the first conductive unit 111, a first insulating portion 1131 is disposed between the first portion 1111 and the second conductive unit 112; and the first insulating portion 1131 is made of insulating material. This arrangement further separates the first polarity electrical pulse applied by the first conductive unit 111 from the second polarity electrical pulse applied by the second conductive unit 112, forming two spaced pulse application regions. Furthermore, by changing the length of the first insulating portion 1131, the range of the electric field application region can be adjusted, thereby affecting the application range of the electrical pulse.

[0057] The above details the specific implementation scheme of the photoelectric co-application device that can simultaneously apply electrical pulses of different polarities. The following will, in conjunction with the accompanying drawings, detail the specific implementation scheme of the photoelectric co-application device that can simultaneously apply multiple beams of light.

[0058] Figure 3 The image shown is yet another photoelectric collaborative application device provided in an embodiment of this disclosure. For example... Figure 3 As shown, at least one radial channel 114 is formed on the side wall of the conductive part 110, and the radial channel 114 extends from the outer wall of the conductive part 110 into the cavity of the conductive part 110. The radial direction of the radial channel 114 may be perpendicular to the radial direction of the conductive part 110.

[0059] Each radial channel 114 houses a light output unit 122, the bottom surface of which is connected to the light transmission unit 121 on the outer surface of the conductive part 110 on the spherical side. Light of a specific wavelength transmitted in the light transmission unit 121 is directed from the bottom of the light output unit 122 toward the hemisphere and refracted on the hemisphere. It is then output to the outside of the device through the radial channel 114, causing the light of the specific wavelength to radiate outward from the radial channel 114 as the center, further expanding the illumination application area of ​​the device and thus expanding the effective range of the device.

[0060] In some embodiments, there are multiple radial channels 114, and the multiple radial channels 114 are located on the same cross section of the conductive portion 110. Figure 4 As shown Figure 3 A schematic cross-sectional view along the direction of the dotted line in the illustrated embodiment. (See diagram below.) Figure 4 As shown, this embodiment includes four radial channels 114, each of which is provided with a corresponding light output unit 122.

[0061] It is understood that the number of radial channels 114 can also be 2, 3, 5, etc., and this disclosure does not impose any specific restrictions on this.

[0062] In some embodiments, the included angle between the radial directions of any adjacent radial channels 114 is the same, such that the radial channels 114 uniformly surround the sidewall of the conductive portion 110.

[0063] Therefore, when the light output unit outputs light of a specific wavelength outward from the radial channel, the light radiating from the radial channel can act evenly on the target area, improving the treatment efficiency of the device.

[0064] In some embodiments, the ends of the cavity of the conductive part 110 and the ends of the radial channel 114 are provided with transparent seals. This prevents blood, tissue fluid, and other substances in the target area from entering the cavity of the conductive part 110 without affecting light exposure, while simultaneously keeping the outer surface of the device smooth.

[0065] In some embodiments, the photoelectric co-application device further includes a blocking plate. During photodynamic therapy, the blocking plate can be applied to the outside of the target area to prevent light of a specific wavelength from irradiating areas outside the target area, thereby preventing healthy tissue cells from being damaged by light irradiation.

[0066] Based on the same inventive concept, this disclosure also provides a photoelectric collaborative application device. Since the principle by which this device solves the problem is similar to that of the above embodiments, the implementation of this device embodiment can refer to the implementation of the above embodiments, and repeated details will not be described again.

[0067] The photoelectric co-application device in this embodiment includes the device provided in any of the above embodiments. In addition, it includes a light-generating unit connected to the illumination unit and an electrical pulse-generating unit connected to the conductive unit. The light-generating unit can generate light of various wavelengths, which is transmitted through the light-conducting unit of the illumination unit to the light-output unit, and then irradiates the target area centered on the light-output unit. The electrical pulse-generating unit can generate electrical pulses of various polarities and pulse parameters (such as pulse amplitude and pulse width), which are applied to the target area through conduction by the conductive unit.

[0068] By adjusting the pulse parameters of the electrical pulse generator, it is possible to generate either reversible or irreversible electroporation pulses. Reversible electroporation pulses can create recoverable micropores on the cell membrane, while irreversible electroporation pulses can create irreversible micropores. The specific pulse parameters are determined by the actual situation.

[0069] Based on the same inventive concept, this disclosure also provides a photoelectric collaborative application method, applied to the photoelectric collaborative application device provided in this disclosure. Since the principle of this method embodiment in solving the problem is similar to that of the above-described device embodiment, the implementation of this method embodiment can refer to the implementation of the above-described device embodiment, and repeated details will not be described again.

[0070] Figure 5 The diagram shown is a schematic flowchart of a photoelectric synergistic application method provided in an embodiment of this disclosure. Figure 5 As shown, the photoelectric synergistic application method provided in this embodiment includes the following steps.

[0071] S510 uses a photoelectric co-application device to apply an electrical pulse to a target area.

[0072] The target area includes tissue cells and photosensitizers free outside the tissue cells. The cell membranes of the tissue cells are disrupted by the electrical pulses applied by the device, creating micropores. The photosensitizers free outside the tissue cells enter the cell interior through the micropores, and the concentration of photosensitizers inside the cells increases accordingly.

[0073] The S520 uses a photoelectric co-application device to output light of a specific wavelength to a target area.

[0074] The wavelength of light should match the photosensitizer inside the cell. When light of a specific wavelength irradiates tissue cells in the target area, the photosensitizer inside the tissue cells reacts with oxygen under light excitation to generate highly reactive reactive oxygen species (ROS). ROS oxidize and damage cellular components such as proteins, nucleic acids, and lipids, inducing apoptosis or necrosis.

[0075] Before applying the electrical pulse, the photoelectric co-application device should be placed in the target area. If the application device can only apply a unipolar electrical pulse, then two application devices should be set up in the target area, one of which applies the first polarity electrical pulse and the other applies the second polarity electrical pulse; if the application device can apply two electrical pulses of opposite polarities simultaneously, then only one application device is needed.

[0076] The electrical pulse waveform of the application device can be diverse. For example, a first polarity electrical pulse and a second polarity electrical pulse can be applied simultaneously; alternatively, the first polarity electrical pulse and the second polarity electrical pulse can be applied sequentially, with the durations of the first and second polarity electrical pulses partially overlapping. Furthermore, by changing the pulse parameters of the first and second polarity electrical pulses, high-voltage narrow pulse sequences and low-voltage wide pulse sequences can be applied continuously or alternately. This further enhances the destructive effect of the electrical pulses on the cell membrane. It is understood that the above methods of applying electrical pulses are merely exemplary and should not be construed as limiting the scope of this disclosure.

[0077] In addition, a barrier plate can be applied to the edge of the target area before applying light to prevent healthy tissue cells from being damaged by light.

[0078] In some embodiments, applying an electrical pulse to a target region using a photoelectric co-application device includes applying a reversible electroporation pulse to the target region using the photoelectric co-application device. The reversible electroporation pulse can generate recoverable micropores on the cell membrane. Therefore, within a certain period after the application of the reversible electroporation pulse, the micropores will recover to an intact cell membrane. At this time, the cell interior contains more photosensitizer, which undergoes a chemical reaction to kill tissue cells under irradiation with light of a specific wavelength; compared to the case without an electrical pulse, its killing effect is stronger; at the same time, because the micropores caused by the reversible electroporation pulse can recover, the safety of this method is improved.

[0079] In some embodiments, after a period of time following the application of the reversible electroporation pulse, the method further includes applying an irreversible electroporation pulse to the target region using a photoelectric co-application device. The irreversible electroporation pulse can create irreversible micropores on the cell membrane, thereby further killing cells in the target region. Therefore, the irreversible electroporation pulse can further destroy target cell tissues that have not been killed by the photosensitized reaction, thereby improving the lethality of the method in this embodiment.

[0080] In some embodiments, the application of light and the application of electrical pulses are relatively independent. Therefore, light of a specific wavelength can be continuously applied before, during, and after the application of the electrical pulses. The specific implementation depends on the actual situation.

[0081] In some embodiments, applying an electrical pulse to a target region using a photoelectric synergistic application device includes: applying an irreversible electroporation pulse to the target region using the photoelectric synergistic application device. In this embodiment, an irreversible electroporation pulse is first applied to the target region, creating irreversible micropores in the cell membrane to kill the cells in the target region; simultaneously, a photosensitizer enters the cell through the micropores. Then, the photosensitizer inside the cell is excited by light, causing the remaining cells to die under the action of a photosensitizing reaction. In the method of this embodiment, the electrical pulse and the photosensitizer work synergistically to kill cells and accelerate cell death.

[0082] It is understood that the above application methods are merely exemplary, and the order of application of reversible electroporation pulses, irreversible electroporation pulses, and light of specific wavelengths can be set according to the actual situation. Two specific implementation methods are described below.

[0083] like Figure 6 As shown in the embodiments of this disclosure, another photoelectric synergistic application method includes the following steps.

[0084] S610 uses a photoelectric co-application device to apply reversible electroporation pulses to a target area.

[0085] The S620 uses a photoelectric co-application device to output light of a specific wavelength to a target area.

[0086] S630 uses a photoelectric co-application device to apply irreversible electroporation pulses to a target area.

[0087] In this implementation, reversible electroporation pulses create recoverable micropores in the cell membrane, effectively temporarily opening channels in the cell membrane and allowing photosensitizers to more easily enter. Within a short period after the pulse application ends, the micropores in the cell membrane recover. Then, light of a specific wavelength irradiates the target area, causing cell death due to the photosensitivity reaction. Finally, irreversible electroporation pulses are applied to the target area, creating multiple irreversible micropores in the cell membrane, further killing any surviving cells from the photosensitivity reaction; during this process, the specific wavelength of light can continue to irradiate the area.

[0088] Therefore, this implementation can largely kill tissue cells in the target area. Furthermore, the electrical pulse is applied in two stages, which enhances its safety.

[0089] The following describes another specific implementation method. For example... Figure 7 As shown in the embodiments of this disclosure, another photoelectric synergistic application method includes the following steps.

[0090] S710 uses a photoelectric co-application device to apply irreversible electroporation pulses to a target area.

[0091] The S720 uses a photoelectric co-application device to output light of a specific wavelength to a target area.

[0092] In this implementation, the irreversible electroporation pulse first creates irreversible micropores in the cell membrane to kill cells in the target area; simultaneously, a photosensitizer enters the cell through the micropores. Then, light of a specific wavelength irradiates the target area, further killing any surviving cells from the electroporation pulse, and the photosensitivity reaction accelerates the death of cells with irreversible micropores. Therefore, this implementation can kill a significant number of cells in the target area with higher efficiency.

[0093] Those skilled in the art will understand that aspects of this disclosure can be implemented as a system, method, or program product. When the program product is run on a terminal device such as an optoelectronic co-application device, the program code is used to cause the terminal device to perform the steps of the various exemplary embodiments of this disclosure described in the above-described method embodiments section of this specification.

[0094] Other embodiments of this disclosure will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This disclosure is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not disclosed herein. The specification and examples are to be considered exemplary only, and the true scope and spirit of this disclosure are indicated by the appended claims.

Claims

1. A photoelectric co-application device, characterized in that, include: A conductive part with a cavity is used to apply an electrical pulse to a target area; An illumination section is disposed in the cavity of the conductive section and is used to apply light to the target area; The illumination section includes a light transmission unit and at least one light output unit connected to the light transmission unit, wherein the at least one light output unit is used to output light of a specific wavelength transmitted in the light transmission unit; The conductive part includes a first conductive unit having a cavity, the cavity extending through one end of the first conductive unit; the at least one light output unit includes a first light output unit, the first light output unit being disposed at the end of the cavity of the first conductive unit; The conductive part further includes: The second conductive unit is sleeved around the first conductive unit; An insulating unit is disposed between the first conductive unit and the second conductive unit to electrically isolate the first conductive unit and the second conductive unit; The first conductive unit is used to apply a first polarity electrical pulse to the target area, and the second conductive unit is used to apply a second polarity electrical pulse to the target area or to ground the second conductive unit. The polarities of the first polarity electrical pulse and the second polarity electrical pulse are opposite.

2. The apparatus according to claim 1, characterized in that, The first conductive unit includes a first part and a second part connected axially, wherein the outer diameter of the first part is larger than the outer diameter of the second part; The insulating unit includes a first insulating part and a second insulating part, the first insulating part and the second insulating part are sleeved on the periphery of the second part, and the second conductive unit is sleeved on the periphery of the second insulating part, wherein, in the axial direction of the first conductive unit, the first insulating part is disposed between the first part and the second conductive unit.

3. The apparatus according to claim 1, characterized in that, The end of the first conductive unit on one side of the first light output unit is a pointed tip with an inclined cut.

4. The apparatus according to claim 1, characterized in that, The conductive portion further includes at least one radial channel formed on the sidewall of the conductive portion, each of the radial channels accommodating the light output unit.

5. The apparatus according to claim 4, characterized in that, The number of radial channels is multiple, and the multiple radial channels are located on the same cross section of the conductive part, and the cross section is perpendicular to the radial direction of the conductive part.

6. The apparatus according to claim 4, characterized in that, The included angle between any two adjacent radial channels is the same.

7. The apparatus according to claim 4, characterized in that, The ends of the cavity of the conductive part and the ends of the radial channel are both provided with transparent seals.

8. The apparatus according to claim 1, characterized in that, It also includes a baffle plate for directing the light emitted by the at least one light output unit to the target area.

9. A photoelectric co-generation device, characterized in that, include: The photoelectric co-application device according to any one of claims 1 to 8; A light-generating unit, connected to the light-illuminating unit in the photoelectric co-application device, is used to generate light of a specific wavelength; An electrical pulse generator, connected to a conductive part in the photoelectric co-application device, is used to generate electrical pulses.

10. A program product, characterized in that, When the program product is run, the program code causes the photoelectric collaborative application device according to any one of claims 1 to 8 to execute the photoelectric collaborative application method, the photoelectric collaborative application method comprising: Using the aforementioned photoelectric co-application device, an electrical pulse is applied to the target region to generate micropores on the cell membrane of the target region; Using the photoelectric co-application device, light of a specific wavelength is output to the target area so that the photosensitizer in the target area combines with the light of the specific wavelength and becomes excited.

11. The program product according to claim 10, characterized in that, The method of applying an electrical pulse to a target area using the photoelectric co-application device includes: Using the aforementioned photoelectric co-application device, a reversible electroporation pulse is applied to the target region, the reversible electroporation pulse being used to generate recoverable micropores on the cell membrane of the target region.

12. The program product according to claim 11, characterized in that, After applying the reversible electroporation pulse to the target area using the photoelectric co-application device, the method further includes: Using the aforementioned photoelectric co-application device, an irreversible electroporation pulse is applied to the target region, the irreversible electroporation pulse being used to generate irreversible micropores on the cell membrane of the target region.

13. The program product according to claim 10, characterized in that, The method of applying an electrical pulse to a target area using the photoelectric co-application device includes: Using the aforementioned photoelectric co-application device, an irreversible electroporation pulse is applied to the target area.

14. The program product according to claim 10, characterized in that, The irradiation time of the light of the specific wavelength includes at least one of the following: before the application of the electric pulse, during the application of the electric pulse, synchronously with the application of the electric pulse, and after the application of the electric pulse.

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