Application of paenibacillus chibensis space mutant in sesame production

Abstract

The application discloses application of a space mutant of Paenibacillus chibensis in sesame production, and provides a new application of a space mutant △PS04-17 strain of Paenibacillus chibensis, research shows that the fermentation liquor of the △PS04-17 strain is used for root soaking and spraying treatment in the growth period of sesame, can promote the development of sesame root system, strengthen the growth of sesame, promote the growth of sesame, significantly increase the number of sesame pods and the weight of sesame grains, and can also increase the yield of sesame by 30% through field yield test results. Meanwhile, the space mutant can also prevent the occurrence of sesame diseases, reduce the incidence of sesame diseases, improve the prevention efficiency of the space mutant on the bacterial wilt and stem spot wilt of sesame, the relative prevention efficiency of the space mutant on the bacterial wilt of sesame reaches 45.00+ / -6.95%, the relative prevention efficiency of the space mutant on the stem spot wilt of sesame reaches 48.18+ / -5.83%, the space mutant can prevent the early decline of sesame plants, can be used for green prevention and control of sesame, and has important significance for quality improvement and yield increase of sesame.

Description

Technical Field

[0001] This invention belongs to the field of agricultural microbiology technology. More specifically, it relates to the application of a space mutant of Bacillus creboni in sesame production. Background Technology

[0002] Oilseed crops refer to crops used for vegetable oil production. Their primary purpose is to extract vegetable oil for use as edible oil, industrial raw materials, and energy. They are important sources of edible oils and feed protein. Oilseed crops play a vital role in human life and industrial production. Soybeans, peanuts, rapeseed, and sesame are the four most important oilseed crops in my country, accounting for 90% of the country's cultivated oilseed crop area. In terms of seed oil content, sesame seeds have an oil content of 50-60%, ranking among the highest of these oilseed crops.

[0003] Sesame, as one of the four major edible oil crops in China, has high application value and plays an important role in crop cultivation due to its short growth period. However, there are still many problems in sesame production in my country. For example, continuous cropping and improper cropping arrangements are important factors limiting the improvement of sesame yield and quality. Continuous cropping leads to increased disease and nutrient imbalance, especially in major producing areas where the area of ​​continuous cropping is large, making diseases such as sesame wilt, bacterial wilt, and stem blight difficult to control, resulting in low and unstable yields. Sesame has many diseases, but stem blight and bacterial wilt are the most prevalent and serious. Stem blight, also known as "black root blight" or "black stalk blight," generally reduces sesame yield by about 10% under normal circumstances, and in severe cases, it can reduce yield by more than half. The earlier the onset, the greater the loss, resulting in decreased sesame quality and yield, and reduced oil content. Sesame bacterial wilt, also known as "black stem disease" or "black stalk disease," or "sesame wilt," can cause widespread death in severe cases. It can reduce yield by 10%-20% in mild cases and more than half in severe cases, resulting in total crop failure in the most severe cases, demonstrating the extent of its damage.

[0004] There is an urgent need to improve sesame yield and quality, and reduce the impact of continuous cropping and diseases on sesame. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the above-mentioned defects and deficiencies and provide the application of Bacillus creboni space mutant in sesame production.

[0006] The purpose of this invention is to provide Cribbenoid Bacillus ( Paenibacillus kribbensis A new application of the space mutant strain △PS04-17 in sesame production.

[0007] Another objective of this invention is to provide a method for promoting sesame growth, increasing sesame yield, or improving sesame quality.

[0008] Another objective of this invention is to provide a method for preventing or reducing the incidence of sesame diseases.

[0009] The above-mentioned objective of this invention is achieved through the following technical solution:

[0010] This invention provides Cribbenoid Bacillus ( Paenibacillus kribbensis A novel application of the space mutant strain △PS04-17 in sesame production has been discovered. This strain was deposited on March 10, 2022, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC NO: 62233), located at No. 100, Xianlie Middle Road, Guangzhou. Research shows that using a microbial agent containing the fermentation broth of the Cribbella space mutant strain △PS04-17, applied through root drenching and spraying during the sesame growing season, can significantly promote sesame root development, enhance sesame growth, increase the number of pods and the weight and quantity of sesame seeds, and ultimately increase sesame yield. The △PS04-17 strain promotes plant growth, increases sesame yield, and improves sesame quality. Furthermore, treatment with the △PS04-17 fermentation broth can also prevent sesame diseases such as bacterial wilt. Ralstonia solanacearum ) and sesame stem spot blight ( Macrophoma sesami The occurrence of △PS04-17 strain fermentation liquid can prevent premature aging of sesame plants. Furthermore, according to the disease incidence survey, treatment with △PS04-17 strain fermentation liquid can also reduce the incidence of sesame diseases. The relative control efficacy against sesame bacterial wilt reached 45.00±6.95%, and the relative control efficacy against sesame stem spot blight reached 48.18±5.83%.

[0011] Therefore, the present invention provides Cribbenoid Bacillus ( Paenibacillus kribbensis The following applications of the space mutant strain △PS04-17:

[0012] Application in promoting sesame growth;

[0013] Application in increasing sesame yield;

[0014] Application in improving sesame quality;

[0015] Application in the preparation of sesame growth promoters;

[0016] Application in the prevention and control of sesame diseases;

[0017] Application in reducing the incidence of sesame diseases.

[0018] This invention provides a method for promoting sesame growth, increasing sesame yield, or improving sesame quality by treating sesame with a microbial agent containing fermentation broth of Bacillus cristatus space mutant strain △PS04-17.

[0019] Preferably, the specific method of use is as follows: the microbial agent containing the fermentation broth of the Cribben-type Bacillus aerospace mutant strain △PS04-17 is applied to the roots and leaves during the sesame growing season.

[0020] This invention also provides a method for preventing or reducing the incidence of sesame diseases, which uses the fermentation broth of the Cricket Bacillus space mutant strain △PS04-17, diluted 75 times, to treat sesame plants by root drenching and foliar spraying.

[0021] Preferably, the fermentation broth concentration of the Cribben-like Bacillus space mutant strain △PS04-17 is not less than 1×10⁻⁶. 6 CFU / mL.

[0022] Preferably, the root drenching and foliar spraying treatment methods are as follows: during the sesame growing season, root drenching is performed once, with an interval of 15 days; then foliar spraying is performed twice, with an interval of 15 days.

[0023] Furthermore, the preparation method of the fermentation broth of the △PS04-17 strain is as follows: the △PS04-17 bacterial solution is inoculated into the fermentation medium at an inoculation amount of 1-10%, and fermented at 25-30℃ and 150-200 rpm for 7 days.

[0024] Furthermore, the sesame disease mentioned is sesame bacterial wilt (… Ralstonia solanacearum ) and / or sesame stem spot blight ( Macrophoma sesami ).

[0025] The present invention has the following beneficial effects:

[0026] This invention provides a novel application of the space-mutated mutant strain △PS04-17 of Bacillus creboni in sesame production. Treatment of sesame with the fermentation broth of this strain effectively promotes plant growth, increases yield, and significantly improves both production and quality. Simultaneously, it reduces plant disease incidence and prevents bacterial wilt and stem blight in sesame, thus preventing premature aging of the plants. Applying strain △PS04-17 to sesame production not only reduces the use of chemical pesticides but also contributes to food safety and environmental sustainability. Its ability to prevent premature aging in sesame, representing a green control measure, is of great significance for improving the quality and efficiency of sesame production. Attached Figure Description

[0027] Figure 1 The study compared the fresh weight of sesame plants between the control and treatment groups; CK was the control group, and HTB was the treatment group.

[0028] Figure 2 This is a comparison of sesame plants between the control group and the treatment group; CK is the control group, and HTB is the treatment group.

[0029] Figure 3 The study compared the fresh weight of sesame roots between the control group and the treatment group; CK was the control group, and HTB was the treatment group.

[0030] Figure 4 The comparison is between the sesame roots of the control group and the treatment group; CK is the control group and HTB is the treatment group.

[0031] Figure 5 This study compares the number of sesame seeds between the control group and the treatment group; CK represents the control group, and HTB represents the treatment group.

[0032] Figure 6 This study compares the number of sesame pods between the control group and the treatment group; CK represents the control group, and HTB represents the treatment group.

[0033] Figure 7 The comparison is between the control group and the treatment group for sesame pods; CK is the control group and HTB is the treatment group.

[0034] Figure 8 The comparison of sesame seed weight between the control group and the treatment group; CK is the control group, and HTB is the treatment group.

[0035] Figure 9 This is a comparison of the average weight of sesame seeds between the control group and the treatment group; CK is the control group, and HTB is the treatment group.

[0036] Figure 10 This study compares the yield of sesame plots between the control and treatment groups. CK represents the control group, and HTB represents the treatment group.

[0037] Figure 11 Comparison of bacterial wilt and stem spot disease incidence in sesame during the flowering and capsule-setting and grain-filling stages and the treatment stages. CK was the control group, and HTB was the treatment group.

[0038] Figure 12 This study compares the relative efficacy of disease control for sesame during the flowering and capsule-forming stages with that during the grain-filling and ripening stages. Detailed Implementation

[0039] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0040] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0041] Example 1: Preparation of Cribbella-like Bacillus inoculum

[0042] The present invention uses Cribbella ( Paenibacillus kribbensisThe space-induced mutation strain △PS04-17 is the research result of the applicant's research group. It was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 17, 2022, with the accession number GDMCC NO: 62233, and is recorded in the prior art: CN 114480222 A (patent publication number).

[0043] Preparation of microbial inoculum: A 1% inoculum of △PS04-17 bacterial suspension was inoculated into a modified Czapek's liquid medium (sodium nitrate 1.5 g / L, dipotassium hydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, potassium chloride 0.5 g / L, ferrous sulfate 0.01 g / L, sucrose 30 g / L). Fermentation was carried out at 25–30℃ and 150 rpm for 7 days to prepare a fermentation broth containing the space-induced mutant strain △PS04-17 of Bacillus creboni. The concentration of the fermentation broth was 1×10⁻⁶. 6 CFU / mL.

[0044] Experiment Example 2: Application in Sesame Growth

[0045] The microbial agent containing the space-induced mutant strain △PS04-17 of Bacillus crippans prepared in Example 1 (hereinafter referred to as Aerospace Treasure) was used in a field trial. The field trial was conducted in Qianfang Town, Jinxian County, Jiangxi Province, with sesame as the crop, variety Ganzhi 7.

[0046] In late May, apply 30 kg of 45% Stanley potassium sulfate compound fertilizer (15-15-15) per mu (approximately 0.067 hectares), followed by rotary tillage. In early June, create raised beds, each 30 m² in size. 2 (4 m × 7.5 m), row sowing is used in each plot, with 12 rows per plot, and the seed amount per acre is approximately 300 g. After sowing, spray 90 mL of 720 g / L metolachlor per acre, diluted in 15 kg of water, as a pre-emergence herbicide treatment.

[0047] The plot was divided into two groups: a control group (CK) and a treatment group (HTB). The CK group received conventional fertilization, while the HTB group received conventional fertilization plus Aerospace Treasure, that is, Aerospace Treasure was applied on the basis of conventional fertilization. The timing and amount of conventional fertilization were the same. Apart from the difference of whether Aerospace Treasure was applied, the field management of the two groups was the same.

[0048] Starting from June 27th, the HTB group was treated with a root drench once every 15 days using 200 mL of Aerospace Treasure diluted in 15 kg of water (75 times dilution); starting from July 27th, the group was treated with a foliar spray using 200 mL of Aerospace Treasure diluted in 15 kg of water every 15 days. No other pesticides were used for pest and disease control in the plots during the trial period.

[0049] Sesame yield was measured using a 3-point random sampling method at harvest time. Sesame biological traits were measured and statistically analyzed: fresh weight per plant, fresh weight per root, number of pods per plant, number of sesame seeds per pod, number of sesame seeds and weight. The results are shown in Table 1 below.

[0050] Table 1 Effects of different treatments on the biological traits of sesame

[0051]

[0052] As shown in Table 1, the fresh weight of individual plants in the HTB group treated with the microbial agent Aerospace Treasure reached over 95 g, with an average of 101.51 g, while the fresh weight of individual plants in the CK group ranged from 40 g to 43.76 g, showing a significant difference. p <0.05)( Figure 1 The results of the comparison of sesame plants are as follows: Figure 2 As shown, the fresh weight of individual roots in the HTB group was over 9 g, while the fresh weight of individual roots in the CK group fluctuated around 5 g, and the difference was significant. p <0.05)( Figure 3 The results of the comparison of sesame roots are as follows: Figure 4 As shown, the number of sesame seeds in the pods of the HTB group was all above 90, with some exceeding 100, while the number of sesame seeds in the pods of the CK group was between 70 and 79. The difference was statistically significant. p <0.05)( Figure 5 The average number of pods per plant in the HTB group was approximately 21, while the average number in the CK group was 16, showing a significant difference. p <0.05)( Figure 6 The results of the comparison of sesame pods are as follows: Figure 7 As shown, the average weight of sesame seeds in the HTB group was approximately 0.34 g, while the average weight of sesame seeds in the CK group was approximately 0.17 g, showing a significant difference. p <0.05)( Figure 8 The average weight of sesame seeds in the CK group was approximately 0.0025 g, while the average weight of sesame seeds in the HTB group was approximately 0.0036 g, showing a significant difference. p <0.05)( Figure 9 ).

[0053] In summary, the results show that the HTB group treated with the microbial agent Aerospace Treasure was superior to the CK group without the agent in terms of single plant fresh weight, single plant root fresh weight, number of pods per plant, number of sesame seeds per pod, and sesame seed weight. It can significantly promote the development of sesame roots, enhance the growth of sesame, and significantly increase the number of sesame pods and the weight of sesame seeds. This indicates that the space-induced mutant strain △PS04-17 of Bacillus crebae plays a role in improving the quality and yield of sesame plants.

[0054] Further statistical analysis of the sesame yield in the treated plots was conducted, and the results are shown in Table 2 below.

[0055] Table 2. Production Statistics of the Community

[0056]

[0057] Table 2 shows that there were significant differences in sesame yield among different treatments. The total yield of the HTB plot treated with the microbial inoculant Aerospace Treasure reached 12.70 kg, with an average yield of approximately 0.14 kg / m², while the total yield of the CK plot was 8.50 kg, with an average yield of approximately 0.09 kg / m². The HTB group yielded 4.20 kg more than the CK plot, and the difference was statistically significant. p <0.05%. The yield of HTB was equivalent to 94.10 kg / mu, while the yield of the CK group was equivalent to 63.00 kg / mu. The yield of the HTB group increased by 31.10 kg / mu compared to the CK group. Figure 10 The results showed that the Aerospace Treasure treatment had a significant effect on increasing sesame yield, increasing it by up to 30%.

[0058] Example 3: Application in the control of sesame diseases

[0059] During the sesame field trial, the occurrence of sesame diseases was investigated and statistically analyzed during the growth and fertilization period after sowing. The main focus was on common sesame field diseases, including bacterial wilt. Ralstonia solanacearum ) and sesame stem spot blight ( Macrophoma sesami Disease surveys were conducted during the flowering and seed-setting stages and the grain-filling and ripening stages of sesame, and the statistical results are shown in Table 3 below.

[0060] Table 3. Statistics on the number of sesame plants affected by diseases

[0061]

[0062] Table 3 shows that during the flowering and capsule-forming stage of sesame, the average incidence of bacterial wilt in the CK group was 1.30%, and the average incidence of stem blight was 1.01%. In the HTB group, the average incidence of bacterial wilt was approximately 0.68%, and the average incidence of stem blight was approximately 0.51%. The incidence rates of bacterial wilt and stem blight in the HTB group decreased by 0.62% and 0.50%, respectively. During the grain-filling and ripening stage of sesame, the average incidence of bacterial wilt in the CK group was approximately 8.73%, and the average incidence of stem blight was approximately 5.07%. In contrast, the average incidence of bacterial wilt in the HTB group (treated with the fungicide) was approximately 4.74%, and the average incidence of stem blight was approximately 2.61%. The incidence rates of bacterial wilt and stem blight in the HTB group decreased by 3.99% and 2.46%, respectively. The statistical results of diseases in different treatment groups are as follows: Figure 11 As shown, the application of Aerospace Treasure has a significant preventive effect against bacterial wilt and stem spot disease in sesame.

[0063] Further statistical analysis was conducted on the relative control efficacy against sesame diseases. The relative control efficacy (%) was calculated as follows: (incidence rate in the control area - incidence rate in the treatment area) / incidence rate in the control area × 100%. The statistical results are shown in Table 4 below.

[0064] Table 4. Relative control efficacy against sesame diseases

[0065]

[0066] Table 4 shows that the relative control efficacy of the microbial inoculant Aerospace Treasure against bacterial wilt of sesame plants reached 47.12±19.82% during the flowering and capsule-setting stage, and the average relative control efficacy reached 45.00±6.95% during the grain-filling and ripening stage. The relative control efficacy against stem blight of sesame plants reached an average of 49.80±5.26% during the flowering and capsule-setting stage, and 48.18±5.83% during the grain-filling and ripening stage. The statistical results of the relative control efficacy of different treatment groups are as follows: Figure 12 As shown.

[0067] In summary, the microbial agent prepared using the space-induced mutant strain △PS04-17 of Bacillus cristatus in this invention can effectively promote sesame plant growth, increase sesame yield, and significantly improve both yield and quality. Simultaneously, it can reduce plant disease incidence and prevent the occurrence of bacterial wilt and stem blight in sesame, preventing premature aging of sesame plants. Disease incidence surveys showed that treatment with △PS04-17 fermentation broth achieved a relative control efficacy of 45.00±6.95% against bacterial wilt and 48.18±5.83% against stem blight. This not only reduces pesticide use but also contributes to food and environmental safety, making it suitable for green pest control in sesame and of great significance for improving the quality and efficiency of sesame production.

[0068] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. Cribbenoid Bacillus ( Paenibacillus kribbensis The application of the space mutant strain △PS04-17 in promoting sesame growth is characterized by, The Kreben Bacillus space mutant △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number GDMCC NO: 62233.

2. Application of the Cribbenoid Bacillus space mutant strain △PS04-17 in increasing sesame yield, characterized in that, The Kreben Bacillus space mutant △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number GDMCC NO: 62233.

3. The application of the Cribbenoid Bacillus space mutant strain △PS04-17 in the preparation of a sesame growth promoter, characterized in that, The Kreben Bacillus space mutant △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number GDMCC NO: 62233.

4. Application of the Cribbella aerospace mutant strain △PS04-17 in the control of sesame diseases, characterized in that, The *Bacillus cristatus* space mutant △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number GDMCC NO: 62233; the sesame disease is sesame bacterial wilt (…). Ralstonia solanacearum ) and / or sesame stem spot blight ( Macrophoma sesami ).

5. The application of the Cribbella aerospace mutant strain △PS04-17 in reducing the incidence of sesame diseases, characterized in that, The *Bacillus cristatus* space mutant △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number GDMCC NO: 62233; the sesame disease is sesame bacterial wilt (…). Ralstonia solanacearum ) and / or sesame stem spot blight ( Macrophoma sesami ).

6. A method for promoting sesame growth and increasing sesame yield, characterized in that, Sesame seeds were treated with a microbial agent containing the fermentation broth of Bacillus cristatus space mutant strain △PS04-17; the Bacillus cristatus space mutant strain △PS04-17 was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 10, 2022, with accession number: GDMCC NO: 62233.

7. A method for preventing or reducing the incidence of sesame diseases, characterized in that, The fermentation broth of the Bacillus cristatus space mutant strain △PS04-17 was used to treat sesame plants by root drenching and foliar spraying. The Bacillus cristatus space mutant △PS04-17 was deposited at the Guangdong Provincial Microbial Culture Collection Center on March 10, 2022, with accession number GDMCC NO: 62233. The sesame disease mentioned was bacterial wilt (sesame bacterial wilt). Ralstonia solanacearum ) and / or sesame stem spot blight ( Macrophoma sesami ).

Images