A composition with soothing, anti-inflammatory, anti-wrinkle and firming efficacy and a process for its preparation

Abstract

The application discloses a composition with soothing anti-inflammatory anti-wrinkle firming efficacy and a preparation method thereof, and the composition comprises a TRPV1 inhibitor, an extract of Glycyrrhiza inflata Bat., palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7. The four raw materials are compounded to have a synergistic effect, reduce the release of pro-inflammatory mediators and inhibit the high reactivity of nerve fibers to achieve soothing anti-inflammatory and anti-irritation; promote the generation of self collagen and sodium hyaluronate, reduce the oxidative stress response to achieve the anti-wrinkle firming efficacy and delay skin aging. The composition has comprehensive effects in soothing, anti-inflammatory, anti-irritation, anti-oxidation, anti-wrinkle and firming, reduces the generation of inflammation and delays skin aging, relieves the burning and stinging sensation of the skin at the same time, and meets the requirements of safety and high efficiency pursued by sensitive skin groups.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics, specifically relating to a composition with soothing, anti-inflammatory, anti-wrinkle, and firming effects, and its preparation method. Background Technology

[0002] Sensitive skin is a skin type that reacts more easily to external stimuli. It is caused by a variety of factors, including genetic factors, climate change, environmental pollution, allergens, and the use of irritating cosmetics or skincare products. Clinically, sensitive skin is characterized by subjective symptoms such as stinging, burning, tightness, or itching when stimulated by physical, chemical, or psychological factors, often accompanied by objective signs such as inflammation and erythema.

[0003] The mechanisms underlying sensitive skin primarily involve two aspects: firstly, the hyperresponsiveness of sensory neurons in the skin; and secondly, impaired skin barrier function. Sensitive skin is often accompanied by an overreaction of peripheral sensory neurons, particularly painful skin reactions such as burning and itching caused by stimulation of transient receptor potential vanillic acid subtype 1 receptors (TRPV1). On the other hand, impaired skin barrier function in sensitive skin reduces protection for nerve endings, making TRPV1 receptors more susceptible to activation by changes in the external environment. When TRPV1 receptors are activated, calcium ions influx in, depolarization occurs, action potentials are generated, and neurotransmitter transmission mediates the afferent sensations of pain, itching, heat, and chemical stimuli.

[0004] Compared to normal skin, sensitive skin has a damaged skin barrier, making it more susceptible to irritation and inflammatory responses. After inflammation causes tissue damage, inflammatory cells (such as macrophages and neutrophils) release specific enzymes called matrix metalloproteinases (MMPs). These MMPs are directed to the damaged tissue and degrade collagen, further exacerbating inflammation. Simultaneously, collagen degradation leads to loss of skin elasticity, resulting in wrinkles, age spots, sagging, and other signs of skin aging. Furthermore, chronic inflammation triggers excessive free radical production, damaging cell structure and DNA, and harming collagen and elastin in the skin. This damage leads to wrinkles, sagging, and loss of elasticity, accelerating the skin aging process. Chronic inflammation also affects the production and elimination of the skin's natural moisturizing factors, causing faster moisture loss. Skin lacking sufficient moisture appears dry, rough, and dull, increasing the risk of fine lines and wrinkles.

[0005] Currently, there is a wide variety of cosmetics on the market targeting sensitive skin. Most products offer only a single function and often cause allergic reactions, itching, and pain in users with sensitive skin. Given these issues and the increasing anti-aging needs of people with sensitive skin, the development of a product that simultaneously soothes, reduces inflammation, provides antioxidant benefits, reduces wrinkles, firms, and combats aging is currently essential in the market. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a composition with soothing, anti-inflammatory, anti-wrinkle, and firming effects. By inhibiting the release of pro-inflammatory factors, counteracting the hyperreactivity of nerve fibers, promoting collagen secretion, inhibiting collagen degradation, and reducing the body's oxidative stress response, it alleviates skin inflammation and increases collagen content. This composition is of great significance for the anti-inflammatory and anti-aging applications in people with sensitive skin.

[0007] The present invention provides a composition with soothing, anti-inflammatory, anti-wrinkle and firming effects, comprising: 0.1-5 parts of TRPV1 inhibitor, 0.1-5 parts of Glycyrrhiza uralensis root extract, 0.01-5 parts of palmitoyl tripeptide-1 and 0.01-5 parts of palmitoyl tetrapeptide-7.

[0008] The composition provided by this invention, which has soothing, anti-inflammatory, anti-wrinkle and firming effects, combines four ingredients: TRPV1 inhibitor, Glycyrrhiza uralensis root extract, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7. By targeting multiple points, they work synergistically to alleviate skin inflammation and signs of aging in multiple dimensions.

[0009] Preferably, the TRPV1 inhibitor is one or more of acetyl dipeptide-1 cetyl ester, pentapeptide-1, and hydrolyzed algae extract.

[0010] Preferably, the composition further comprises 0.01-3 parts of a small molecule acid, wherein the small molecule acid includes one or more of lactic acid, glycolic acid and mandelic acid.

[0011] Preferably, the composition further comprises 1-10 parts of 1,2-pentanediol.

[0012] Preferably, the composition further comprises 60-90 parts of C12-15 alcohol benzoate.

[0013] Preferably, the composition further comprises 1-20 parts of phenoxyethanol.

[0014] Preferably, the composition comprises, by weight, 0.1-5 parts acetyl dipeptide-1 cetyl ester, 0.1-5 parts Glycyrrhiza inflata root extract, 0.01-5 parts palmitoyl tripeptide-1, 0.01-5 parts palmitoyl tetrapeptide-7, 0.01-3 parts glycolic acid, 1-10 parts 1,2-pentanediol, 60-90 parts C12-15 benzoate, and 1-20 parts phenoxyethanol.

[0015] Preferably, the composition comprises, by weight, 0.35 parts acetyl dipeptide-1 cetyl ester, 0.8 parts glycyrrhiza uralensis root extract, 0.08 parts palmitoyl tripeptide-1, 0.04 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.23 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0016] This invention provides a method for preparing a composition with soothing, anti-inflammatory, anti-wrinkle, and firming effects, comprising the following steps:

[0017] Step 1: Mix the root extract of Glycyrrhiza uralensis and palmitoyl tripeptide-1, heat and then keep at a constant temperature to obtain the first solution;

[0018] Step 2: Heat palmitoyl tetrapeptide-7 to a constant temperature and add it to the first solution to obtain the second solution;

[0019] Step 3: Cool the solution obtained in Step 2, add the TRPV1 inhibitor to the second solution, and stir.

[0020] Step 4: The composition is obtained after ultrasonic filtration.

[0021] Preferably, step one further includes the addition of 1,2-pentanediol and phenoxyethanol, wherein,

[0022] The root extract of Glycyrrhiza inflata, palmitoyl tripeptide-1, 1,2-pentanediol and phenoxyethanol were mixed, heated and kept at a constant temperature to obtain the first solution.

[0023] Preferably, step two further includes the addition of a small molecule acid and a C12-15 alcohol benzoate, wherein,

[0024] The small molecule acid, palmitoyl tetrapeptide-7, and C12-15 alcohol benzoate were mixed, heated and kept at a constant temperature, and then added to the first solution to obtain the second solution.

[0025] Preferably, in step one, the temperature is raised to 70-80°C and held for 20-30 minutes; in step two, the temperature is raised to 70-80°C and held for 20-30 minutes; and in step three, the temperature is lowered to 35-40°C.

[0026] Preferably, the present invention provides a method for preparing a composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, comprising the following steps:

[0027] Step 1: Mix the root extract of Glycyrrhiza uralensis, palmitoyl tripeptide-1, 1,2-pentanediol and phenoxyethanol, heat to 70-80℃, and hold at that temperature for 20-30 minutes to obtain the first solution.

[0028] Step 2: Mix glycolic acid, palmitoyl tetrapeptide-7 and C12-15 benzoate, heat to 70-80°C, hold at that temperature for 20-30 minutes, and add to the first solution to obtain the second solution;

[0029] Step 3: When the solution obtained in Step 2 is cooled to 35-40℃, add acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0030] Step 4: The composition is obtained after ultrasonic filtration.

[0031] More preferably, the present invention provides a method for preparing the soothing, anti-inflammatory, anti-wrinkle, and firming composition as described above, comprising the following steps:

[0032] Step 1: Stir 0.8 parts of Glycyrrhiza uralensis root extract, 0.08 parts of palmitoyl tripeptide-1, 10 parts of 1,2-pentanediol, and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution.

[0033] Step 2: Stir 0.5 parts glycolic acid, 0.04 parts palmitoyl tetrapeptide-7, and 73.23 parts C12-15 benzoic acid ester and heat to 70°C, hold at that temperature for 30 minutes, then add to the first solution to obtain the second solution;

[0034] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0035] Step 4: The composition is obtained after ultrasonic filtration.

[0036] To better understand this invention, a detailed explanation of the specific mechanism is provided below.

[0037] Oxidative stress is a major factor contributing to inflammatory responses. Oxidative stress occurs when cells are subjected to external damage or when endogenous factors (such as metabolic processes) generate oxygen free radicals that exceed the cell's antioxidant defense capacity. Oxidative stress can then activate inflammatory responses, leading to inflammation-related diseases and symptoms. Oxidative stress is also considered an important mechanism in the aging process. With age and changes in metabolism, the cell's ability to scavenge oxygen free radicals gradually weakens, leading to increased oxidative stress. This results in the accumulation of oxidative damage to cells, affecting cell function and tissue health, ultimately leading to aging. Antioxidants have the potential to inhibit oxidative stress, reduce inflammation, and delay aging. Through in-depth research on various natural plant extracts with antioxidant activity, the inventors discovered that *Glycyrrhiza inflata* root extract possesses superior antioxidant activity, which can help maintain the body's health and youthfulness to a certain extent. Combining *Glycyrrhiza inflata* root extract, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7 significantly enhances the anti-inflammatory and anti-aging effects of the composition.

[0038] In the soothing, anti-inflammatory, anti-wrinkle, and firming composition of this invention, acetyl dipeptide-1 cetyl ester, as an outstanding TRPV1 receptor antagonist, has the effect of timely soothing the skin, relieving burning and stinging sensations, and increasing the skin's tolerance threshold. Simultaneously, it can stimulate elastin synthesis to combat skin laxity. Licorice root extract has excellent free radical scavenging ability, preventing oxidative damage to the skin caused by external stimuli, and can significantly inhibit inflammatory factors, soothe skin irritation, and improve facial redness. Palmitoyl tripeptide-1 stimulates collagen and elastin production to improve skin elasticity and firmness, increase skin water content and moisture retention, enhance epidermal structure function, strengthen the skin barrier function, and effectively prevent and reduce wrinkles. Palmitoyl tetrapeptide-7 stimulates the regeneration of collagen fibers in the dermis, increases the content of sodium hyaluronate in the skin, improves the epidermal barrier function, and can inhibit the immune response caused by inflammation in the skin. By regulating intercellular signaling pathways, it reduces the release of inflammatory factors, thereby alleviating inflammatory reactions such as redness, itching, and discomfort.

[0039] The composition of this invention inhibits the release of inflammatory factors and activation of TRPV1 receptors, which can alleviate skin inflammation, reduce facial redness and irritation, and reduce the production of metalloproteinases, thereby reducing collagen degradation and wrinkle formation. At the same time, it reduces oxidative stress, stimulates the production of collagen and sodium hyaluronate, repairs the skin barrier function, delays skin aging, and further inhibits inflammation, thus addressing the skin care pain points of sensitive skin people who are more easily irritated and more prone to aging from multiple dimensions.

[0040] Compared with the prior art, the present invention has the following technical advantages:

[0041] 1. The composition of the present invention can effectively soothe sensitive skin, inhibit inflammatory factors, stimulate collagen production, and meet the anti-inflammatory and anti-aging needs of sensitive skin.

[0042] 2. The licorice root extract selected by the present invention has a strong antioxidant capacity, which can effectively inhibit the oxidation reaction of free radicals in the body and significantly improve the anti-inflammatory and anti-aging effects of the composition.

[0043] 3. This invention creatively uses phenoxyethanol, 1,2-pentanediol, and C12-15 benzoate as solvents to fully dissolve licorice root extract, acetyl dipeptide-1 cetyl ester, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7, forming a composition with soothing, anti-inflammatory, anti-wrinkle, and firming effects.

[0044] 4. The composition of the present invention is an oil-based composition, and its small molecule active substances are more easily absorbed through the skin and reach the dermis layer of the skin more quickly to exert their effects, avoiding the potential harm caused by the use of penetration enhancers. Attached Figure Description

[0045] Figure 1 A bar chart showing the distance traveled by zebrafish embryos under high temperature.

[0046] Figure 2 The bar chart shows the relative expression levels of type I collagen genes in zebrafish.

[0047] Figure 3 A bar chart showing the intensity of reactive oxygen species (ROS) signals in zebrafish embryos. Detailed Implementation

[0048] It should be noted that, unless otherwise specified, the embodiments and features described in this invention can be combined with each other. The effects of the invention will be further illustrated below with reference to the embodiments. It should be understood that the embodiments described in this specification are merely for explaining this application and are not intended to limit this application.

[0049] Example 1

[0050] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.4 parts Glycyrrhiza uralensis root extract, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.75 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0051] The preparation method of the above composition includes the following steps:

[0052] Step 1: Stir 0.4 parts of Glycyrrhiza uralensis root extract, 10 parts of 1,2-pentanediol, and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution;

[0053] Step 2: Stir 0.5 parts of glycolic acid and 73.75 parts of C12-15 benzoic acid ester and heat to 70°C, keep at the temperature for 30 minutes, and add to the first solution to obtain the second solution;

[0054] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0055] Step 4: The composition is obtained after ultrasonic filtration.

[0056] Example 2

[0057] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.8 parts glycyrrhiza uralensis root extract, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.35 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0058] The preparation method is the same as in Example 1.

[0059] Example 3

[0060] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.04 parts palmitoyl tripeptide-1, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.11 parts C12-15 benzoyl alcohol, and 15 parts phenoxyethanol.

[0061] The preparation method of the above composition includes the following steps:

[0062] Step 1: Stir 0.04 parts palmitoyl tripeptide-1, 10 parts 1,2-pentanediol and 15 parts phenoxyethanol and heat to 75°C, hold at this temperature for 25 minutes to obtain the first solution;

[0063] Step 2: Stir 0.5 parts glycolic acid and 74.11 parts C12-15 benzoic acid ester and heat to 70°C, keep at the temperature for 30 minutes, and add to the first solution to obtain the second solution;

[0064] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0065] Step 4: The composition is obtained after ultrasonic filtration.

[0066] Example 4

[0067] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.08 parts palmitoyl tripeptide-1, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.07 parts C12-15 benzoyl alcohol, and 15 parts phenoxyethanol.

[0068] The preparation method is the same as in Example 3.

[0069] Example 5

[0070] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.02 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.13 parts C12-15 benzoyl alcohol, and 15 parts phenoxyethanol.

[0071] The preparation method of the above composition includes the following steps:

[0072] Step 1: Stir 10 parts of 1,2-pentanediol and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution;

[0073] Step 2: Stir 0.5 parts glycolic acid, 0.02 parts palmitoyl tetrapeptide-7, and 74.13 parts C12-15 benzoic acid ester and heat to 70°C, hold at this temperature for 30 minutes, then add to the first solution to obtain the second solution;

[0074] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0075] Step 4: The composition is obtained after ultrasonic filtration.

[0076] Example 6

[0077] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.04 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.11 parts C12-15 benzoyl alcohol, and 15 parts phenoxyethanol.

[0078] The preparation method is the same as in Example 5.

[0079] Example 7

[0080] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.4 parts glycyrrhiza uralensis root extract, 0.04 parts palmitoyl tripeptide-1, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.71 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0081] The preparation method of the above composition includes the following steps:

[0082] Step 1: Stir 0.4 parts of Glycyrrhiza uralensis root extract, 0.04 parts of palmitoyl tripeptide-1, 10 parts of 1,2-pentanediol, and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution.

[0083] Step 2: Stir 0.5 parts of glycolic acid and 73.71 parts of C12-15 alcohol benzoate and heat to 70°C, keep at the temperature for 30 minutes, and add to the first solution 1 to obtain the second solution;

[0084] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0085] Step 4: The composition is obtained after ultrasonic filtration.

[0086] Example 8

[0087] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.8 parts glycyrrhiza uralensis root extract, 0.08 parts palmitoyl tripeptide-1, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.27 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0088] The preparation method is the same as in Example 7.

[0089] Example 9

[0090] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.4 parts glycyrrhiza uralensis root extract, 0.02 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.73 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0091] The preparation method of the above composition includes the following steps:

[0092] Step 1: Stir 0.4 parts of Glycyrrhiza uralensis root extract, 10 parts of 1,2-pentanediol, and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution;

[0093] Step 2: Stir 0.5 parts glycolic acid, 0.02 parts palmitoyl tetrapeptide-7, and 73.73 parts C12-15 benzoic acid ester and heat to 70°C, hold at that temperature for 30 minutes, and add to the first solution to obtain the second solution;

[0094] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0095] Step 4: The composition is obtained after ultrasonic filtration.

[0096] Example 10

[0097] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.8 parts glycyrrhiza uralensis root extract, 0.04 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.31 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0098] The preparation method is the same as in Example 9.

[0099] Example 11

[0100] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.04 parts palmitoyl tripeptide-1, 0.02 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.09 parts C12-15 benzoyl alcohol, and 15 parts phenoxyethanol.

[0101] The preparation method of the above composition includes the following steps:

[0102] Step 1: Stir 0.04 parts palmitoyl tripeptide-1, 10 parts 1,2-pentanediol and 15 parts phenoxyethanol and heat to 75°C, hold at this temperature for 25 minutes to obtain the first solution;

[0103] Step 2: Stir 0.5 parts glycolic acid, 0.02 parts palmitoyl tetrapeptide-7, and 74.09 parts C12-15 benzoic acid ester and heat to 70°C, hold at that temperature for 30 minutes, then add to the first solution to obtain the second solution;

[0104] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0105] Step 4: The composition is obtained after ultrasonic filtration.

[0106] Example 12

[0107] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.08 parts palmitoyl tripeptide-1, 0.04 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 74.03 parts C12-15 benzoyl ester, and 15 parts phenoxyethanol.

[0108] The preparation method is the same as in Example 11.

[0109] Example 13

[0110] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.4 parts Glycyrrhiza inflata root extract, 0.04 parts palmitoyl tripeptide-1, 0.02 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.69 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0111] The preparation method of the above composition includes the following steps:

[0112] Step 1: Stir 0.4 parts of Glycyrrhiza uralensis root extract, 0.04 parts of palmitoyl tripeptide-1, 10 parts of 1,2-pentanediol, and 15 parts of phenoxyethanol and heat to 75°C, hold at that temperature for 25 minutes to obtain the first solution.

[0113] Step 2: Stir 0.5 parts glycolic acid, 0.02 parts palmitoyl tetrapeptide-7, and 73.69 parts C12-15 benzoic acid ester and heat to 70°C, hold at that temperature for 30 minutes, then add to the first solution to obtain the second solution;

[0114] Step 3: When the temperature drops to 35°C, add 0.35 parts of acetyl dipeptide-1 cetyl ester to the second solution and stir.

[0115] Step 4: The composition is obtained after ultrasonic filtration.

[0116] Example 14

[0117] A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, said composition being made from the following ingredients in weight percentages: 0.35 parts acetyl dipeptide-1 cetyl ester, 0.8 parts glycyrrhiza uralensis root extract, 0.08 parts palmitoyl tripeptide-1, 0.04 parts palmitoyl tetrapeptide-7, 0.5 parts glycolic acid, 10 parts 1,2-pentanediol, 73.23 parts C12-15 benzoate, and 15 parts phenoxyethanol.

[0118] The preparation method is the same as in Example 13.

[0119] Test Example 1

[0120] The samples prepared in the above embodiments were used for cell experiments to test the expression of IL-6 and TNF-α.

[0121] Inoculation: 1×10 4 Normal human fibroblasts were seeded into 96-well plates at a seeding density of cells / well and incubated overnight in an incubator (37°C, 5% CO2).

[0122] Drug administration: When the cell deposition rate in the 96-well plate reached 40-50%, drug administration was performed in groups. The samples prepared in the above examples were added to the groups, with the blank control group receiving no sample. Each group had 4 replicates. After drug administration, the 96-well plates were placed in an incubator (37°C, 5% CO2) for 24 hours.

[0123] UVA irradiation: According to the experimental group, the UVA irradiation group was subjected to UVA irradiation and placed in an incubator (37℃, 5% CO2) for 24 hours.

[0124] Cell collection: Collect the cell supernatant into EP tubes and freeze at -80°C after collection.

[0125] The ELISA kit was followed sequentially, and the levels of IL-6 and TNF-α in the fibroblast supernatant were measured using a microplate reader. The inhibition of IL-6 and TNF-α by each sample group is shown in Table 1.

[0126] Table 1. Different embodiments inhibit the secretion of inflammatory factors IL-6 and TNF-α in human fibroblasts induced by UVA.

[0127]

[0128]

[0129] Table 1 shows that the combination of acetyl dipeptide-1 cetyl ester, *Glycyrrhiza inflata* root extract, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7 exhibits excellent inhibitory effects on the inflammatory factors IL-6 and TNF-α, significantly superior to the inhibitory effects of any single ingredient. This indicates a significant synergistic effect among these four components in inhibiting IL-6 and TNF-α. Furthermore, increasing the proportion of these four components further enhances the inhibitory effect on the inflammatory factors.

[0130] Test Example 2

[0131] Performance testing was conducted using Example 14.

[0132] Example 14 was aliquoted into four centrifuge tubes and labeled as samples A to D. Sample A was placed at room temperature, sample B at -20°C, sample C at 50°C, and sample D was placed in a high and low temperature thermal cycling test chamber (-20°C for 24 hours, 25°C for 24 hours, 50°C for 24 hours, -20°C for 24 hours, with 4 days as one cycle). After 84 days, the four samples were taken out and their properties and colors were observed to see if there were any significant changes, or if there was any precipitation or stratification. If there were no significant differences, it indicated that the samples had good thermal cycling stability.

[0133] Samples A through D showed no precipitation or stratification, and exhibited good stability under thermal cycling.

[0134] Example 14 was placed in a centrifuge at 5000 r / min and centrifuged for 60 min. After centrifugation, it was observed whether any precipitate was formed. If no precipitate was formed, it indicated that the sample had good centrifugal stability.

[0135] Example 14 was clear, without precipitation, and showed good centrifugal stability.

[0136] Test Example 3

[0137] Example 14 was selected for testing on the reduction of zebrafish embryo movement distance under high temperature.

[0138] Transient receptor potential vanilloid 1 (TRPV1) is a key ion channel protein widely expressed in sensory neurons. TRPV1 is a thermosensitive ion channel that can be activated by heat stimulation and specific chemicals. The primary function of TRPV1 is to transmit signals related to pain and temperature perception. When tissues are stimulated or damaged, certain chemicals are released that can bind to and activate TRPV1 channels, leading to sensations of pain and burning in sensory neurons. Besides pain and temperature perception, TRPV1 is also involved in other physiological processes. For example, its expression in skin cells is associated with skin sensitivity and itching. Zebrafish also possess the TRPV1 pathway, which is activated at high temperatures, peaking at 37°C or above, resulting in a significant increase in swimming speed. Therefore, using zebrafish activated by high temperatures is ideal for testing soothing tingling tests. The soothing effect of the test sample is evaluated by measuring the distance the zebrafish embryos move and calculating the rate of reduction in movement distance.

[0139] Sixteen 5-day-old zebrafish were exposed to a 1% concentration of the sample solution added in Example 14 in a 96-well plate, with a model control group also included. After 2 hours of exposure at 28°C, the zebrafish were placed in a DanioVision zebrafish motion recorder preheated to 38°C to record their swimming trajectory over 5 minutes in a dark environment as their distance, which was then statistically analyzed. The test results are shown below. Figure 1 .

[0140] Compared with the blank control group, 1% Example 14 reduced the movement distance of zebrafish embryos by 29% (p = 0.0063). 1% Example 14 can significantly shorten the movement distance of zebrafish embryos at high temperatures and has a soothing effect on stinging.

[0141] Test Example 4

[0142] Example 14 was selected for a test to promote the expression of type I collagen genes in zebrafish.

[0143] Collagen is the most abundant extracellular matrix protein in the human body, with type I collagen being the most common type, accounting for 90% of the body's collagen production. Type I collagen is a natural antioxidant that helps maintain skin hydration and elasticity, reduces wrinkles, and replaces collagen fibers lost due to light. With age, adult skin gradually loses type I collagen, leading to decreased skin elasticity and causing wrinkles, sagging, and other signs of aging. The distribution of type I collagen in zebrafish is similar to that in humans and shows a high degree of conservation. Testing the expression of zebrafish type I collagen genes (col1a1a, col1a1b, and col1a2) and calculating the type I collagen gene expression promotion rate can evaluate the anti-wrinkle and firming effects of the tested samples.

[0144] Thirty-six six-day-old zebrafish were divided into three groups and exposed to a 1% concentration of the sample solution added in Example 14. A blank control group was also included. After 24 hours of exposure, RNA was extracted from the zebrafish, cDNA was synthesized, and real-time PCR amplification was performed. β-actin was used as the housekeeping gene, and Ct was used as the amplification result. The relative expression levels of col1a1a, col1a1b, and col1a2 were calculated and statistically analyzed. The test results are shown in Table 2 and... Figure 2 .

[0145] Compared with the blank control group, the 1% Example 14 significantly promoted the expression of zebrafish col1a1a, col1a1b, and col1a2 genes by 31% (p = 0.00082), 73% (p = 0.0023), and 178% (p = 0.0014), respectively. The 1% Example 14 significantly promoted the expression of zebrafish type I collagen genes, demonstrating a promotion of type I collagen regeneration and achieving anti-wrinkle and firming effects.

[0146] Table 2. Results of the test on promoting type I collagen gene expression in zebrafish

[0147]

[0148] Test Example 5

[0149] Example 14 was selected for zebrafish embryo reactive oxygen species (ROS) scavenging test.

[0150] Reactive oxygen species (ROS) are byproducts of normal aerobic metabolism in the body. Under normal circumstances, a certain amount of ROS within cells is necessary, playing an important role in cell signaling and regulating cell proliferation. However, excessive ROS can damage cell structure and function. When the body is exposed to stimuli such as ultraviolet radiation or heat, ROS levels increase dramatically, exceeding the body's own clearance capacity. This leads to an imbalance in the body's oxidation-antioxidant processes, resulting in oxidative stress. Consequently, various cellular biomolecules (DNA, lipids, and proteins) suffer oxidative damage. Accumulated oxidative damage leads to decreased cell function and ultimately cell death. Cell death causes abnormal skin aging, resulting in loss of skin elasticity and wrinkles. The ROS regulation mechanism in zebrafish is the same as in humans. Using specific reactive oxygen species fluorescent staining reagents, intracellular ROS in zebrafish embryonic cells can be efficiently and specifically labeled, and ROS clearance rates can be calculated to evaluate the anti-wrinkle and firming effects of the test samples.

[0151] Twenty-four 48-hour-old zebrafish embryos were exposed to a 1% concentration of the sample solution added in Example 14, with a blank control group included. After 24 hours of exposure, the embryos were stained with H2DCFDA, and the ROS signal intensity was measured by fluorescence imaging and statistically analyzed. The test results are shown below. Figure 3 .

[0152] Compared with the blank control group, 1% Example 14 showed a 49% ROS scavenging rate in zebrafish embryos (p = 0.000000000000000099). 1% Example 14 significantly scavenged ROS in zebrafish embryos, demonstrating antioxidant effects and achieving anti-wrinkle and firming benefits.

[0153] Table 3. Results of reactive oxygen species (ROS) scavenging test in zebrafish embryos

[0154]

[0155] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to the embodiments, those skilled in the art should understand that modifications or equivalent substitutions to the technical solutions of the present invention do not depart from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A composition having soothing, anti-inflammatory, anti-wrinkle, and firming effects, characterized in that, The composition comprises the following components: 0.1-5 parts TRPV1 inhibitor, 0.1-5 parts Glycyrrhiza uralensis root extract, 0.01-5 parts palmitoyl tripeptide-1, 0.01-5 parts palmitoyl tetrapeptide-7, 0.01-3 parts small molecule acid, 1-10 parts 1,2-pentanediol, 1-20 parts phenoxyethanol, and 60-90 parts C12-15 benzoyl alcohol ester; The TRPV1 inhibitor is acetyl dipeptide-1 cetyl ester; The small molecule acid is glycolic acid.

2. A method for preparing the composition with soothing, anti-inflammatory, anti-wrinkle, and firming effects as described in claim 1, comprising the following steps: Step 1: Mix the root extract of Glycyrrhiza uralensis inflata, palmitoyl tripeptide-1, 1,2-pentanediol and phenoxyethanol, heat and then keep at a constant temperature to obtain the first solution; Step 2: Mix the small molecule acid, palmitoyl tetrapeptide-7, and C12-15 benzoyl alcohol, heat and maintain the temperature, then add to the first solution to obtain the second solution; Step 3: Cool the solution obtained in Step 2, add the TRPV1 inhibitor to the second solution, and stir. Step 4: The composition is obtained after ultrasonic filtration.

3. The preparation method according to claim 2, characterized in that, In step one, the temperature is raised to 70-80℃ and held for 20-30 minutes; in step two, the temperature is raised to 70-80℃ and held for 20-30 minutes; in step three, the temperature is lowered to 35-40℃.

Images