Application of serine in the diet of Coccinella septempunctata, diet of Coccinella septempunctata and preparation method thereof

Abstract

The present invention discloses the application of serine in the feed of Coccinella septempunctata, belonging to the field of feed. It also discloses the feed of Coccinella septempunctata added with serine, and its raw material composition is: dried fly maggot pupae powder, dried goose liver powder, rose pollen, dried kiwifruit powder, sucrose, olive oil, purified water, potassium sorbate, serine. Through the improvement of materials, the present invention improves the feeding effect of female Coccinella septempunctata, and by controlling the content of serine, more precise control is carried out on the egg-laying amount, feeding frequency, egg hatching rate and adult survival rate, improving the survival rate, egg hatching rate and egg-laying amount of Coccinella septempunctata, which is more beneficial to the feeding of female Coccinella septempunctata, and the cost is greatly reduced compared with the prior art.

Description

Technical Field

[0001] The present invention relates to the field of feeds, and particularly to the application of serine in the feed of Coccinella septempunctata, the feed of Coccinella septempunctata and its preparation method. Background Art

[0002] Coccinella septempunctata is widely used for the biological control of agricultural and forestry pests. Feeding Coccinella septempunctata only with aphids will cause problems such as occupying a large amount of space, consuming a large amount of manpower, material resources, electricity, etc., which limits the large-scale production of Coccinella septempunctata. Developing artificial feed is the basis for improving the feeding efficiency of Coccinella septempunctata. The raw materials of the artificial feed for Coccinella septempunctata are rich in resources, suitable in price and simple to store. Chinese patent applications CN115024449A and CN115039846A respectively disclose that estrogen and androgen are used in the artificial feed for feeding Coccinella septempunctata. However, from the current situation of feeding Coccinella septempunctata with artificial feed, there are still problems such as the relatively high cost of the artificial feed for Coccinella septempunctata. Summary of the Invention

[0003] One of the purposes of the present invention is to provide the application of serine in the feed of Coccinella septempunctata to solve the above problems.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is as follows: The application of serine in the feed of Coccinella septempunctata, wherein the serine is used as an additive in the artificial feed for female Coccinella septempunctata, and the serine is L-serine.

[0005] As a preferred technical solution, the concentration of the serine in the artificial feed is 20 - 80 mg / L.

[0006] It should be noted that: The artificial feed of the present invention is composed of dry powder feed and liquid feed, and serine is added to the liquid feed, so the unit is mg / L.

[0007] As a further preferred technical solution, the concentration of L-serine in the artificial feed is 40 mg / L.

[0008] As a preferred technical solution, the L-serine is used to increase the egg-laying amount, hatching rate or feeding frequency of Coccinella septempunctata.

[0009] Another purpose of the present invention is to provide a feed for Coccinella septempunctata added with L-serine. The technical solution adopted is that it is composed of the following raw materials in parts by weight: 25 - 38 parts of dry fly maggot pupae powder, 15 - 30 parts of dry goose liver powder, 18 - 26 parts of rose pollen, 8 - 16 parts of dry kiwifruit powder, 12 - 19 parts of sucrose, 8 - 16 parts of olive oil, 80 - 100 parts of purified water, 0.5 - 1.5 parts of potassium sorbate, and L-serine with a concentration of 5 - 80 mg / L.

[0010] The third object of the present invention is to provide a method for preparing the above-mentioned feed, comprising the following steps:

[0011] (1) Weigh dry fly maggot pupae powder, dry goose liver powder, rose pollen, dry kiwifruit powder, sucrose and olive oil, mix and stir evenly to obtain a mixture, which is the dry feed.

[0012] (2) Mix sucrose, purified water, potassium sorbate and L-serine, and stir evenly to obtain a liquid feed after stirring.

[0013] As a preferred technical solution, in step (2), a magnetic stirrer is used for stirring, the stirring speed is 115 - 125 r / min, and the stirring time is 15 - 25 min to ensure sufficient stirring.

[0014] As a preferred technical solution: The paste-like artificial feed is wrapped and sealed with a sealing film, and then stored in a refrigerator for later use to prevent contamination and moisture loss.

[0015] As a preferred technical solution, the temperature of the refrigerator is 3 - 5 °C to prevent the artificial feed from deteriorating due to excessive temperature.

[0016] Compared with the prior art, the advantages of the present invention are as follows: The present invention innovatively uses L-serine as an additive in the artificial feed for female Coccinella septempunctata, and further screens its appropriate concentration to obtain an artificial feed for female Coccinella septempunctata containing L-serine additive; through the improvement of materials, the feeding effect of female Coccinella septempunctata is improved, and by controlling the content of serine, the egg production, feeding frequency, egg hatching rate and adult survival rate are more precisely controlled, improving the survival rate, egg hatching rate and egg production of Coccinella septempunctata, which is more conducive to the feeding of female Coccinella septempunctata; the artificial feed containing L-serine achieves a feeding effect similar to that of the natural feed of aphids; more importantly, compared with the reported artificial feed added with estrogen and androgen, the cost of the present invention is reduced by about 50%, showing very significant technical effects.

[0017] It should be noted that: Aphids are the natural prey of Coccinella septempunctata, and the feeding effect of the artificial feed cannot exceed that of the aphid feed for the time. However, the breeding of aphids is affected by various factors (temperature, light, humidity, space, etc.), and it also requires a large amount of manpower, material resources and electricity; while the raw materials of the artificial feed are rich, the price is appropriate, and the breeding space is small. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Table showing the effect of artificial feeds containing different concentrations of L-serine on the egg production of Coccinella septempunctata;

[0019] Figure 2Table showing the effect of artificial diets containing different concentrations of L-serine on the hatching rate of Coccinella septempunctata

[0020] Figure 3 Table showing the effect of artificial diets containing different concentrations of L-serine on the feeding frequency of adult Coccinella septempunctata

[0021] Figure 4 Table showing the effect of artificial diets containing different concentrations of L-serine on the survival rate of adult Coccinella septempunctata Detailed implementation manners

[0022] The present invention will be further described below in conjunction with the accompanying drawings.

[0023] Example 1:

[0024] The artificial diet for female Coccinella septempunctata containing L-serine as an additive consists of the following raw materials in parts by weight: 30 parts of dried fly maggot pupae powder, 20 parts of dried goose liver powder, 21 parts of rose pollen, 10 parts of dried kiwifruit powder, 15 parts of sucrose, 10 parts of olive oil, 90 parts of purified water, 1 part of potassium sorbate, and L-serine with a concentration of 20 mg / L;

[0025] The preparation method of the new additive for the above artificial diet for female Coccinella septempunctata includes the following steps:

[0026] (1) Place the fly maggot pupae and goose liver in a blender respectively and crush them into powder. Weigh the dried fly maggot pupae powder, dried goose liver powder, rose pollen, dried kiwifruit powder, sucrose and olive oil, mix them in a blender and stir evenly to obtain a mixture;

[0027] (2) Put all the mixture in step (1) into a beaker, add purified water, potassium sorbate and L-serine, and stir evenly with a magnetic stirrer at a stirring speed of 125 r / min for 15 min to form a paste-like artificial diet containing the additive.

[0028] Example 2:

[0029] Compared with Example 1, the added amount of L-serine in this example is 40 mg / L, and the rest is the same as in Example 1.

[0030] Example 3:

[0031] Compared with Example 1, the added amount of L-serine in this example is 80 mg / L, and the rest is the same as in Example 1.

[0032] Effect test example

[0033] Experimental screening of the feeding effect of different concentrations of L-serine additives on Coccinella septempunctata

[0034] In this experiment, the effects of L-serine additives at 5 different concentration gradients on the feeding indexes of the ladybug, such as fecundity, hatching rate, adult feeding frequency, and adult survival rate, were measured respectively. The purpose was to clarify the effects of L-serine additives at different concentrations on the reproduction of the ladybug and screen for the appropriate concentration of additives for artificial feed.

[0035] 1) Test method: Take the following raw materials in parts by weight: 30 parts of fly maggot pupa dry powder, 20 parts of goose liver dry powder, 21 parts of rose pollen, 10 parts of kiwifruit dry powder, 15 parts of sucrose, 10 parts of olive oil, 90 parts of purified water, and 1 part of potassium sorbate. According to the preparation method of the embodiment, an appropriate amount of L-serine was added to prepare a paste-like artificial diet for female ladybugs with additives at concentrations of 0, 5, 10, 20, 40, and 80 mg / L. The experiment was carried out under the conditions of an indoor temperature of 25±1°C, a humidity of 75%, and a light intensity of 16 h / d.

[0036] Pair the mated male and female adults individually in a petri dish (diameter 9 cm), and place 0.2 g of the paste-like artificial diet in a plastic lid (diameter 1 cm) to feed the adults. Replace the fresh diet once a day to ensure freshness. Set 20 replicates, observe the fecundity of female ladybugs and the hatching rate of eggs until the adults stop laying eggs, and use the group fed with pure aphids as the control group. Observe and collect the egg masses laid by ladybugs in the petri dish at 9:00, 12:00, 14:00, and 17:00 every day, and count the number of eggs per pair of adults.

[0037] Two to three days after the artificial diet is put in, select 150 eggs from each treatment and place them in a petri dish with filter paper at the bottom. Spray water to maintain a certain humidity, and observe the hatching of eggs at 10:00 and 16:00 every day. Timely pick out the hatched larvae to avoid them eating the eggs.

[0038] Starting from the day when the artificial diet is put in, observe once every 10 minutes from 15:00 to 16:00 every day. Record the treatment with feeding behavior as 1 and the one without as 0, and count the feeding frequency of ladybugs in each treatment group.

[0039] Starting from the day when the artificial diet is put in, observe the survival of male and female adults at 10:00 and 16:00 every day. Timely pick out the dead adults, count the number of surviving adults in each treatment group after 60 days, and calculate the survival rate.

[0040] 2) Experimental results:

[0041] As Figure 1As shown in the figure, the results of the artificial diet supplemented with different concentrations of L-serine on the egg-laying of Coccinella septempunctata are shown as follows: During the entire observation period, the average daily egg-laying amounts of the six treatment groups and the control group fed to Coccinella septempunctata decreased from high to low as: control group > 40mg / L > 20mg / L > 80mg / L > 10mg / L > 5mg / L > 0mg / L, which were 31.13, 32.80, 28.75, 27.50, 21.25, 15.13, and 3.00 eggs, respectively. The average daily egg-laying amounts of the Coccinella septempunctata in the 0, 5, and 10mg / L treatment groups were significantly lower than those of the control group (p < 0.05), and there was no significant difference in the average daily egg-laying amounts of the Coccinella septempunctata between the 20, 40, and 80mg / L treatment groups and the control group (P > 0.05).

[0042] As Figure 2 shown in the figure, to clarify the effect of the artificial diet supplemented with different concentrations of L-serine on the egg hatching rate of Coccinella septempunctata, the results of the artificial diet supplemented with different concentrations of L-serine on the egg hatching rate of Coccinella septempunctata are shown as follows: During the entire recording period, the egg hatching rates of the six treatment groups and the control group fed to Coccinella septempunctata decreased from high to low as: control group > 40mg / L > 80mg / L > 20mg / L > 10mg / L > 5mg / L > 0mg / L, which were 89.31%, 88.63%, 86.49%, 84.78%, 69.29%, 68.58%, and 49.06%, respectively. The egg hatching rates of the Coccinella septempunctata in the 0, 5, and 10mg / L treatment groups were significantly lower than those of the control group (p < 0.05), and there was no significant difference in the egg hatching rates of the Coccinella septempunctata between the 20, 40, and 80mg / L treatment groups and the control group (P > 0.05).

[0043] As Figure 3 shown in the figure, to clarify the effect of the artificial diet supplemented with different concentrations of L-serine on the feeding frequency of adult Coccinella septempunctata, the results of the artificial diet supplemented with different concentrations of L-serine on the feeding frequency of Coccinella septempunctata are shown as follows: During the entire observation period, the average feeding frequencies of the six treatment groups fed to Coccinella septempunctata decreased from high to low as: 40mg / L > 80mg / L > 20mg / L > 10 / L > 5mg / L > 0mg / L, that is, 0.35, 0.30, 0.28, 0.20, 0.19, and 0.12 times / min, respectively.

[0044] As Figure 4 shown in the figure, to clarify the effect of the artificial diet supplemented with different concentrations of L-serine on the survival rate of adult Coccinella septempunctata, the results of the artificial diet supplemented with different concentrations of L-serine on the survival rate of Coccinella septempunctata are shown as follows: During the entire observation period, the survival rates of the six treatment groups and the control group fed to Coccinella septempunctata decreased from high to low as: control group > 40mg / L > 10mg / L > 20mg / L > 80mg / L > 5mg / L > 0mg / L, and there was no significant difference among the six treatment groups and the control group (P > 0.05).

[0045] In summary, adding an appropriate concentration of L-serine to artificial feed has a positive significance for the rearing of Coccinella septempunctata, which can increase the egg production, hatching rate, adult feeding frequency, etc. of Coccinella septempunctata, and will not have an adverse effect on the survival rate of Coccinella septempunctata. The preferred concentration of L-serine is 5-80 mg / L, and the more preferred content is 40 mg / L.

[0046] Comparison of feed costs:

[0047] Taking the purchase of 20 mg of the androgen of CN115039846A or 400 g of serine for 200 yuan as an example, the cost comparison is shown in Table 1.

[0048] Table 1 Cost comparison of different feeds

[0049] Name Volume of feeding solution (L) Feeding time (taking 500 pairs of ladybugs as an example) Average cost (yuan / day) Androgen 20L 40 days 5.00 yuan / day Example 2 40L 80 days 2.50 yuan / day

[0050] As can be seen from Table 1, when feeding Coccinella septempunctata with serine of the present invention, compared with the androgen of the existing literature CN115039846A, under the same egg-laying efficiency, the cost is 50% of that of the androgen, and the present invention significantly reduces the application cost; the cost of the estrogen of CN115024449A is similar to that of the above androgen.

[0051] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. The application of serine in Coccinella septempunctata feed is characterized in that: The serine is used as an additive in artificial feed of female Coccinella septempunctata to increase the egg-laying amount, hatching rate or feeding frequency of the Coccinella septempunctata. The serine is L-serine.

2. The use according to claim 1, characterized in that The concentration of serine in the artificial feed is 5-80 mg / L.

3. The use according to claim 2, characterized in that The concentration of L-serine in the artificial feed is 40 mg / L.

4. The use according to claim 1, characterized in that The artificial feed for female Coccinella septempunctata consists of the following raw materials in parts by weight: 25-38 parts of fly maggot pupa dry powder, 15-30 parts of goose liver dry powder, 18-26 parts of rose pollen, 8-16 parts of kiwi fruit dry powder, 12-19 parts of sucrose, 8-16 parts of olive oil, 80-100 parts of purified water, 0.5-1.5 parts of potassium sorbate, and L-serine with a concentration of 5-80 mg / L.

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