Compositions, cosmetics and methods of making for lifting skin tightness

Abstract

The present application relates to the field of cosmetic technology, in particular to a composition for improving skin firmness, a cosmetic and a preparation method thereof.The composition for improving skin firmness comprises the following components by weight: 0.1-10 parts of golden microalgae, 0.1-20 parts of rice fermentation liquor and 0.01-3 parts of inositol; wherein the fermentation strain of the rice fermentation liquor is yeast.The composition for improving skin firmness is mild and non-irritating, has strong permeability, can be used in cosmetics, enhances the generation of skin collagen and elastic fibers, and effectively improves skin firmness and elasticity.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to compositions, cosmetics and methods for preparing the same for improving skin firmness. Background Technology

[0002] The first sign of skin aging is not wrinkles, but sagging. The causes of skin aging are multifaceted, including both endogenous and exogenous factors. Endogenous factors mainly include a decreased cell renewal rate and reduced ATP function, while exogenous factors include ultraviolet radiation, pollution, and stress. These factors lead to oxidative stress in the skin, which in turn triggers the production of free radicals, damaging cell membranes and skin basal structural proteins, thus accelerating the skin aging process.

[0003] With changes in modern lifestyles and the environment, skin aging and sagging have become a common problem. Anti-aging and improving skin firmness and elasticity are no longer just needs of middle-aged and elderly people. More and more young people are also seeking effective anti-aging products. Therefore, the development of skin care products that improve skin firmness and elasticity is of great significance.

[0004] Traditional skincare products designed to improve skin firmness and elasticity have drawbacks such as high irritation, poor absorption, and limited effectiveness in improving skin firmness, and urgently need improvement. Summary of the Invention

[0005] Based on this, the present invention provides a mild and non-irritating composition with strong permeability that can effectively improve skin firmness. It can be used in cosmetics to enhance the production of collagen and elastin fibers in the skin, thereby effectively improving skin firmness and elasticity.

[0006] The first aspect of this invention provides a composition for improving skin firmness, the technical solution of which is as follows:

[0007] A composition for improving skin firmness comprises the following components in parts by weight:

[0008] 0.1-10 parts of golden microalgae, 0.1-20 parts of rice fermentation liquid, and 0.01-3 parts of inositol;

[0009] The fermentation strain in the rice fermentation liquid is yeast.

[0010] In one embodiment, the composition for improving skin firmness comprises the following components in parts by weight:

[0011] 0.1-6 parts of golden microalgae, 0.5-15 parts of rice fermentation liquid, and 0.05-2 parts of inositol.

[0012] In one embodiment, the composition for improving skin firmness comprises the following components in parts by weight:

[0013] 0.5-6 parts of golden microalgae, 1-12 parts of rice fermentation liquid, and 0.5-2 parts of inositol.

[0014] In one embodiment, the method for preparing the rice fermentation liquid includes the following steps:

[0015] Yeast stock solution and culture medium are mixed and cultured to prepare yeast strain suspension;

[0016] The yeast strain suspension is inoculated into cooked rice grains, and fermentation is carried out to prepare the rice fermentation broth.

[0017] In one embodiment, the yeast is Saccharomyces veronae.

[0018] In one embodiment, the inoculation ratio of the yeast strain suspension to the cooked rice grains is 10wt%~20wt%.

[0019] In one embodiment, the fermentation culture temperature is 30°C to 40°C.

[0020] In one embodiment, the fermentation culture time is 10h to 72h.

[0021] In one embodiment, the composition for improving skin firmness further includes shea butter, jojoba oil, tocopheryl acetate, and behenol.

[0022] In one embodiment, the skin-firming composition comprises, by weight, 0.5-5 parts shea butter, 0.5-5 parts jojoba oil, 1-5 parts tocopheryl acetate, and 2-5 parts behenol.

[0023] In a second aspect, the present invention also provides the application of the composition for improving skin firmness as described above, the technical solution of which is as follows:

[0024] A cosmetic product comprising the composition described above for improving skin firmness.

[0025] In one embodiment, the cosmetic is selected from at least one of face masks, eye creams, face creams, sunscreens, serums, lotions, and toners.

[0026] In one embodiment, the cosmetic is a face cream, comprising the following components by weight percentage:

[0027] The composition for improving skin firmness as described above contains 0.21wt%~53wt% of thickener, 10wt%~20wt% of chelating agent, 0.01wt%~0.2wt% of skin feel modifier, 0.1wt%~1wt% of solubilizer, 0.5wt%~3wt% of solubilizer and 22.8wt%~89.18wt% of solvent.

[0028] In one embodiment, the thickener is selected from one or more of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, carbomer, carboxymethyl cellulose, diatomaceous earth, and titanium dioxide.

[0029] In one embodiment, the chelating agent is selected from one or more of disodium ethylenediaminetetraacetate, sodium ethylenediamine di-o-phenylacetate, and tetrasodium glutamate diacetate.

[0030] In one embodiment, the skin feel modifier is selected from one or more of isononyl isononanoate, isopropyl palmitate, isopropyl myristate, and cyclopentamethoxysiloxane.

[0031] In one embodiment, the solubilizer is selected from one or more of polyethylene glycol stearate, coconut oil diethanolamide, and PEG-160 sorbitan triisostearate.

[0032] In one embodiment, the solvent is water.

[0033] In one embodiment, the face cream comprises the following components by weight percentage:

[0034] The composition includes: 0.1wt%~10wt% golden microalgae, 0.1wt%~20wt% rice fermentation broth, 0.01wt%~3wt% inositol, 0.5wt%~5wt% shea butter, 0.5wt%~5wt% jojoba oil, 1wt%~5wt% tocopheryl acetate, 2wt%~5wt% behenol, 2wt%~5wt% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3wt%~5wt% carbomer, 3.5wt%~6wt% carboxymethyl cellulose, 1wt%~2wt% diatomaceous earth, 0.5wt%~2wt% titanium dioxide, 0.01wt%~0.2wt% disodium ethylenediaminetetraacetate, 0.1wt%~1wt% isononyl isononanoate, 0.5wt%~3wt% polyethylene glycol stearate, and 22.8wt%~85.18wt% water.

[0035] In one embodiment, the cream further includes one or more of a preservative and a fragrance.

[0036] In one embodiment, the preservative is selected from one or both of phenoxyethanol and pentanediol.

[0037] A third aspect of the present invention provides a method for preparing the cosmetic as described above, the technical solution of which is as follows:

[0038] A method for preparing a cosmetic as described above includes the following steps:

[0039] Mix the components together.

[0040] The present invention has at least the following beneficial effects:

[0041] In this invention, golden microalgae, rice fermentation liquid, and inositol are used synergistically to give the composition excellent antioxidant properties, fundamentally protecting the skin from free radical damage. It also enhances collagen and elastin production, resulting in firmer and more elastic skin. Furthermore, it possesses good permeability and biocompatibility, easily penetrating deep into the skin while aiding the absorption of other active ingredients, further enhancing the efficacy of each ingredient. In particular, through synergistic effects, this invention can enhance the effects of inositol in boosting cell metabolism, improving cell nutrition, and increasing elasticity to alleviate sagging. Therefore, the composition provided by this invention can achieve the goal of deeply and multidimensionally improving skin firmness and elasticity.

[0042] In addition, the composition provided by the present invention can also: (1) maintain skin moisture balance, which can alleviate the problems of dry and rough skin; (2) protect; (3) enhance skin barrier function, strengthen the function of keratinocytes and dermal cells, reduce moisture loss, and enhance skin regeneration and repair capabilities; (4) improve uneven skin tone and pigmentation problems; (5) neutralize free radicals, regulate inflammation and oxidative stress pathways, and eliminate the toxicity of exogenous substances.

[0043] Given the numerous advantages of the skin-firming composition described in this invention, it is well-suited for use in the cosmetics field. Furthermore, the preparation method of the cosmetics described in this invention is simple, easy to industrialize, and employs mild reaction conditions with a high degree of mechanization, resulting in excellent reproducibility and stability of both the product quality and the process. Detailed Implementation

[0044] The present invention will be further described in detail below with reference to specific embodiments. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0045] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0046] the term

[0047] Unless otherwise stated or in case of contradiction, the terms or phrases used in this invention shall have the following meanings:

[0048] In this invention, both "above" and "below" include the number itself. For example, "below 1" means ≥1.

[0049] In this invention, numerical ranges are referred to as continuous unless otherwise specified, and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed in this invention should be understood to include any and all subranges to which they are included.

[0050] In this invention, if the unit of a data range is only followed by the right endpoint, it indicates that the units of the left and right endpoints are the same. For example, 800~850nm means that the units of the left endpoint "800" and the right endpoint "850" are both nm (nanometers).

[0051] In this invention, unless otherwise specified, the temperature parameter can be either constant temperature treatment or treatment within a certain temperature range. The constant temperature treatment allows temperature fluctuations within the precision range controlled by the instrument.

[0052] In this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "at least one" means one, two, or more than two; "several" or "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. In the description of this application, "several" means at least one, such as one, two, etc., unless otherwise explicitly specified.

[0053] Unless otherwise specified, all steps of the present invention may be performed sequentially or randomly, preferably sequentially. For example, the method includes steps (a) and (b), indicating that the method may include steps (a) and (b) performed sequentially, or it may include steps (b) and (a) performed sequentially. For example, the mention that the method may also include step (c) indicates that step (c) may be added to the method in any order. For example, the method may include steps (a), (b), and (c), or it may include steps (a), (c), and (b), or it may include steps (c), (a), and (b), etc.

[0054] In this invention, unless otherwise stated, singular terms may include plural terms and should not be construed as having a quantity of one.

[0055] In this invention, %(w / w) and wt% both represent weight percentage, %(v / v) refers to volume percentage, and %(w / v) refers to mass-volume percentage.

[0056] The aging process is influenced by many exogenous stimuli and endogenous cellular factors, with complex mechanisms. Numerous factors lead to oxidative stress in the skin, which in turn triggers the production of free radicals, damaging cell membranes and skin basal structural proteins, thus accelerating the skin aging process. Whether elderly or young, skin aging and sagging have become very common problems.

[0057] Currently, anti-aging products are mainly concentrated in the fields of food, health products, and medical aesthetics. (1) Their anti-aging perspective is relatively singular, or rather, their dimension of improving skin firmness and elasticity is singular. The effect is not ideal or the cost is too high and the side effects are strong. For example, medical aesthetic lifting is expensive and the postoperative recovery period is long. Retinol requires the establishment of tolerance and is highly irritating. (2) The effective ingredients are difficult to penetrate and cannot achieve fundamental improvement. For example, directly supplementing collagen and other peptides is difficult to ensure effective penetration and protein structure integrity as macromolecules. It also has high environmental requirements, and exogenous supplementation is also easy to degrade, which cannot fundamentally improve the elasticity of the skin. Therefore, it is of great significance to develop skin care products that are gentle and non-irritating, have good skin penetration and absorption, and can effectively improve skin firmness and improve skin elasticity in multiple dimensions.

[0058] To address the aforementioned issues, this invention provides a gentle, all-natural, non-irritating composition with high biocompatibility and permeability, which effectively enhances skin firmness and elasticity by synergistically using golden microalgae, rice fermentation liquid, and inositol. This composition can deeply and multidimensionally improve skin firmness and elasticity.

[0059] The technical solution is as follows:

[0060] A composition for improving skin firmness comprises the following components in parts by weight:

[0061] 0.1-10 parts of golden microalgae, 0.1-20 parts of rice fermentation liquid, and 0.01-3 parts of inositol;

[0062] The fermentation strain in the rice fermentation liquid is yeast.

[0063] Microalgae are algae that are adapted to various extreme environments. Golden microalgae contain a variety of active ingredients such as sugars, chlorophyll, amino acids, proteins, minerals, and vitamins. Under the influence of a micro-electric field, they aid in the absorption of active ingredients in skincare products, enhancing their brightening and anti-aging effects. Furthermore, golden microalgae possess strong antioxidant properties. Since human aging is inseparable from the oxidation process, golden microalgae can neutralize free radicals through exogenous antioxidant activity and promote the expression of antioxidant enzyme genes such as SOD for endogenous antioxidant activity, thus achieving a fundamental anti-aging effect. Moreover, golden microalgae also have highly efficient regenerative capabilities. Studies have shown that it can promote the synthesis of dermal fibers such as collagen, inhibit the activity of the enzyme MMP-1 which degrades dermal fibers, and stimulate the expression of regeneration-related genes, thereby accelerating cell repair and regeneration, achieving skin structure regeneration and reconstruction, and ultimately improving skin firmness and anti-aging effects.

[0064] This invention employs a method for producing gold microalgae by co-culturing algae extracts with gold, transforming "mineral gold" into low-irritant, highly nutritious, and highly active bio-gold. The gold content in the gold microalgae ranges from 2 mg / L to 10 mg / L. Simultaneously, the gold microalgae also contain various polysaccharides, oligosaccharides, chlorophyll, amino acids, proteins, minerals, vitamins, and other active ingredients. In particular, the bio-gold under the influence of a micro-electric field helps enhance the absorption of active ingredients in skincare products, further improving brightening and anti-aging effects.

[0065] In the example of this invention, the gold microalgae was purchased from Weidan Biotechnology Co., Ltd.

[0066] In this invention, the fermentation strain of the rice fermentation liquid is yeast.

[0067] In one embodiment, the yeast is *Saccharomyces veronae*.

[0068] In one embodiment, the method for preparing rice fermentation liquid includes the following steps:

[0069] Yeast stock solution and culture medium are mixed and cultured to prepare yeast strain suspension;

[0070] The yeast strain suspension is inoculated into cooked rice grains, and fermentation is carried out to prepare the rice fermentation broth.

[0071] In one embodiment, the parameters for culturing and preparing the yeast strain suspension include: a temperature of 20°C to 30°C and a time of 40h to 150h. Optionally, the temperature for culturing and preparing the yeast strain suspension includes, but is not limited to, 20°C, 22°C, 24°C, 26°C, 28°C, or 30°C. Optionally, the time for culturing and preparing the yeast strain suspension includes, but is not limited to, 40h, 48h, 60h, 72h, 80h, 90h, 100h, 110h, 120h, 130h, 140h, or 150h.

[0072] In one embodiment, the preparation of a yeast strain suspension by mixing yeast stock solution and culture medium includes the following steps:

[0073] Strain activation: Inoculate the selected yeast stock solution into sterilized activation medium and incubate at 28°C for 48 hours for later use;

[0074] Under aseptic conditions, the activated bacterial strain was transferred to the sterilized expansion medium using an inoculation loop and placed in a constant temperature shaker at 150 r / min and 28℃ for 48 hours.

[0075] The expanded strain was inoculated into MRS medium at an inoculation rate of 3% and cultured at 28°C for 48 hours to obtain a yeast suspension.

[0076] In one embodiment, the inoculation ratio of the yeast strain suspension to the cooked rice grains is 15wt% to 20wt%, including but not limited to 15wt%, 16wt%, 18wt%, 19wt%, or 20wt%. Preferably, the inoculation ratio of the yeast strain suspension to the cooked rice grains is 15wt%.

[0077] In one embodiment, the fermentation temperature is 30°C to 40°C, including but not limited to 30°C, 32°C, 35°C, 37°C, 39°C, or 40°C. Preferably, the fermentation temperature is 37°C.

[0078] In one embodiment, the fermentation time is 10h to 72h, including but not limited to 10h, 20h, 30h, 40h, 48h, 60h, or 72h. Preferably, the fermentation time is 48h.

[0079] Understandably, fermentation is carried out in a clean and stable environment. After fermentation, the filtrate can be collected through filtration and used as rice fermentation liquid.

[0080] In one embodiment, the preparation of the rice fermentation broth by inoculating cooked rice grains with the yeast strain suspension and fermenting the rice includes the following steps:

[0081] Wash 20g of selected rice with water, soak for 24 hours, drain and steam in a pot until cooked, then put into a bottle, add deionized water and let stand and cool to room temperature, while loosening the rice grains; transfer 15% of the yeast inoculum suspension into the rice grains and incubate at 37℃ for 2 days to obtain the fermentation liquid; let the fermentation liquid stand, filter and collect the clear liquid to obtain the yeast / rice fermentation product, i.e., rice fermentation liquid.

[0082] Research has found that rice ferment filtrate is a natural skincare ingredient with highly effective and rapid moisturizing properties. It can penetrate deep into the skin to provide long-lasting hydration. A significant characteristic of skin aging is increased moisture loss and decreased water content. Furthermore, rice ferment filtrate possesses excellent skin affinity and permeability (a smaller contact angle indicates better skin affinity; rice ferment filtrate has a contact angle of 82°, while purified water has 102°), which can improve the hydration of the epidermis and help other active ingredients penetrate and absorb.

[0083] Furthermore, rice fermentation broth is rich in biocompatible nutrients, including α-KG, guanosine, adenosine, cytidine, amino acids, polysaccharides, and minerals. Studies have shown that α-KG can promote the proliferation of epidermal keratinocytes, increase the expression of mRNA (FLG, SPT, and IVL) of skin water retention and barrier-related genes in keratinocytes, thereby promoting the synthesis of collagen and elastin fibers, thus enhancing skin firmness and elasticity, and reducing the appearance of fine lines and wrinkles. Adenosine can transfer energy in the form of ATP and ADP to provide cell vitality and inhibit the production of oxygen free radicals.

[0084] Simultaneously, it was found that substances containing adenosine can regulate MMP1 and inhibit its synthesis. MMP-1 is an important protease related to skin aging; increased levels accelerate the breakdown of skin collagen, leading to skin laxity and wrinkles. Guanosine, as an endogenous substance in organisms, participates in the synthesis of nucleic acids and proteins and various life activities. Studies have shown that topical application can directly and rapidly replenish cellular energy, promote the biosynthesis of skin collagen, elastin, and fibrin, and improve skin elasticity and firmness. Cytidine monophosphate (CMP) is also a ubiquitous nucleotide in organisms, an essential substance for cells, and an important intermediate in the conversion into lecithin, which can enhance the regenerative capacity of skin cells.

[0085] Inositol is a widely distributed cyclohexanehexol-type monosaccharide and a 100% naturally sourced vitamin. It participates in carbohydrate and lipid metabolism in the body and is widely used in the food, health products, and medical fields. It is also used in high-end skincare products.

[0086] Specifically, inositol possesses antioxidant and anti-inflammatory properties, protecting skin cells from free radical damage and helping to prevent signs of aging. Studies have shown that inositol can promote energy metabolism in dermal fibroblasts, improve cellular nutrition, and enhance protein synthesis, including structural proteins such as elastin and collagen, which are responsible for dermal structure, integrity, and biomechanical properties, making the skin more elastic and achieving anti-aging from the root while strengthening the skin barrier. Simultaneously, inositol significantly promotes the production of PDGF-BB in dermal fibroblasts, significantly improving skin elasticity. Furthermore, biomechanical tests have revealed that inositol use can significantly enhance elasticity and alleviate sagging, significantly improving skin elasticity recovery rate and elastic deformation rate, restoring the skin to a youthful state. Specifically, the elasticity recovery rate (Ur / Uf) decreases with age, a value correlated with the age of healthy adults. Inositol can also increase the expression of filaggrin in NHEK (normal human epidermal keratinocytes) by up to 65%, promoting barrier maintenance. Silk polymers play an important role in maintaining the skin's barrier function by promoting epidermal differentiation and forming the unique barrier structure of the stratum corneum during the formation of the outer layer of the epidermis.

[0087] Furthermore, as a biocompatible permeability factor, inositol has excellent transdermal permeability, which helps other active ingredients to quickly penetrate deep into the skin. In addition, it can protect keratinocytes from hypertonic pressure caused by dehydration, maintaining the keratinocyte water balance.

[0088] This invention utilizes gold microalgae, rice fermentation liquid, and inositol synergistically to exert effects from multiple angles, simultaneously addressing both endogenous and exogenous factors. It provides a gentle, all-natural, non-irritating composition with high biocompatibility and permeability, effectively enhancing skin firmness and elasticity, thus achieving a deep, multi-dimensional improvement in skin firmness and elasticity. Specifically: (1) Maintaining skin moisture balance can alleviate dryness and roughness; (2) Protection; (3) Enhancing skin barrier function, strengthening keratinocyte and dermal cell function, reducing moisture loss, and improving skin regeneration and repair capabilities; (4) Improving uneven skin tone and pigmentation; (5) Neutralizing free radicals, regulating inflammatory and oxidative stress pathways, and neutralizing the toxicity of exogenous substances; (6) Increasing the content of elastin and collagen in the skin, fundamentally improving skin elasticity and firmness. It is evident that this composition uses highly permeable and biocompatible raw materials to ensure that the active ingredients penetrate into the skin to exert their effects. At the same time, it is all-natural, gentle, and non-irritating. These raw materials and their constituent ingredients are used in very small quantities in skin care products, which enhances the production of collagen and elastin fibers in the skin and effectively improves skin firmness and elasticity.

[0089] In the composition for improving skin firmness of the present invention, the weight parts of golden microalgae are 0.1 to 10 parts, including but not limited to 0.1 parts, 0.5 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts. Preferably, in the composition for improving skin firmness of the present invention, the weight parts of golden microalgae are 0.1 to 6 parts. More preferably, in the composition for improving skin firmness of the present invention, the weight parts of golden microalgae are 0.5 to 6 parts.

[0090] In the composition for improving skin firmness of the present invention, the rice fermentation liquid is present in an amount of 0.1 to 20 parts by weight, including but not limited to 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts by weight. Preferably, in the composition for improving skin firmness of the present invention, the rice fermentation liquid is present in an amount of 0.5 to 15 parts by weight. Further, in the composition for improving skin firmness of the present invention, the rice fermentation liquid is present in an amount of 1 to 12 parts by weight.

[0091] In the composition for improving skin firmness of the present invention, the inositol content is 0.01 to 3 parts by weight, including but not limited to 0.01 parts, 0.05 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.7 parts, 2.8 parts, 2.9 parts, or 3 parts. Preferably, in the composition for improving skin firmness of the present invention, the inositol content is 0.05 to 2 parts by weight. More preferably, in the composition for improving skin firmness of the present invention, the inositol content is 0.5 to 2 parts by weight.

[0092] In one preferred embodiment, the composition for improving skin firmness comprises the following components in parts by weight:

[0093] 0.1-6 parts of golden microalgae, 0.5-15 parts of rice fermentation liquid, and 0.05-2 parts of inositol.

[0094] In one more preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0095] 0.5-6 parts of golden microalgae, 1-12 parts of rice fermentation liquid, and 0.5-2 parts of inositol.

[0096] In one embodiment, the composition for improving skin firmness further includes shea butter, jojoba oil, tocopheryl acetate, and behenol.

[0097] Preferably, the composition for improving skin firmness comprises, by weight, 0.5-5 parts shea butter, 0.5-5 parts jojoba oil, 1-5 parts tocopheryl acetate, and 2-5 parts behenol.

[0098] Understandably, in the skin-firming composition of the present invention, the shea butter is present in 0.5 to 5 parts by weight, including but not limited to 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.5, 4, 4.5, or 5 parts by weight. Preferably, in the skin-firming composition of the present invention, the shea butter is present in 1 to 3 parts by weight.

[0099] Understandably, in the composition for improving skin firmness of the present invention, the weight percentage of jojoba oil is 0.5 to 5 parts, including but not limited to 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.5, 4, 4.5, or 5 parts. Preferably, in the composition for improving skin firmness of the present invention, the weight percentage of jojoba oil is 1 to 2 parts.

[0100] Understandably, in the composition for improving skin firmness of the present invention, the weight parts of tocopherol acetate are 1 to 5 parts, including but not limited to 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.7 parts, 2.8 parts, 2.9 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts. Preferably, in the composition for improving skin firmness of the present invention, the weight parts of tocopherol acetate are 1.5 to 3 parts.

[0101] Understandably, in the composition for improving skin firmness of the present invention, the behenol is present in 2 to 5 parts by weight, including but not limited to 2 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.7 parts, 2.8 parts, 2.9 parts, 3 parts, 3.1 parts, 3.2 parts, 3.3 parts, 3.4 parts, 3.5 parts, 3.6 parts, 3.7 parts, 3.8 parts, 3.9 parts, 4 parts, 4.1 parts, 4.2 parts, 4.5 parts, or 5 parts. Preferably, in the composition for improving skin firmness of the present invention, the behenol is present in 2 to 4 parts by weight.

[0102] In one preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0103] 0.1-10 parts of golden microalgae, 0.1-20 parts of rice fermentation liquid, 0.01-3 parts of inositol, 0.5-5 parts of shea butter, 0.5-5 parts of jojoba oil, 1-5 parts of tocopheryl acetate, and 2-5 parts of behenol.

[0104] In one preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0105] 0.1-6 parts of golden microalgae, 0.5-15 parts of rice fermentation liquid, 0.05-2 parts of inositol, 0.5-5 parts of shea butter, 0.5-5 parts of jojoba oil, 1-5 parts of tocopheryl acetate, and 2-5 parts of behenol.

[0106] In one preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0107] 0.1-6 parts of golden microalgae, 0.5-15 parts of rice fermentation liquid, 0.05-2 parts of inositol, 1-3 parts of shea butter, 1-2 parts of jojoba oil, 1.5-3 parts of tocopheryl acetate, and 2-4 parts of behenol.

[0108] In one preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0109] 0.5-6 parts of golden microalgae, 1-12 parts of rice fermentation liquid, 0.5-2 parts of inositol, 0.5-5 parts of shea butter, 0.5-5 parts of jojoba oil, 1-5 parts of tocopheryl acetate, and 2-5 parts of behenol.

[0110] In one preferred embodiment, the skin-firming composition comprises the following components in parts by weight:

[0111] 0.5-6 parts of golden microalgae, 1-12 parts of rice fermentation liquid, 0.5-2 parts of inositol, 1-3 parts of shea butter, 1-2 parts of jojoba oil, 1.5-3 parts of tocopheryl acetate, and 2-4 parts of behenol.

[0112] In a second aspect, the present invention also provides the application of the composition for improving skin firmness as described above, the technical solution of which is as follows:

[0113] A cosmetic product comprising the composition described above for improving skin firmness.

[0114] In one embodiment, the cosmetic is selected from at least one of face masks, eye creams, face creams, sunscreens, serums, lotions, and toners.

[0115] In one preferred embodiment, the cosmetic is a face cream.

[0116] In one embodiment, the face cream comprises the following components by weight percentage:

[0117] The composition for improving skin firmness as described above contains 0.21wt%~53wt% of thickener, 10wt%~20wt% of chelating agent, 0.01wt%~0.2wt% of skin feel modifier, 0.1wt%~1wt% of solubilizer, 0.5wt%~3wt% of solubilizer and 22.8wt%~89.18wt% of solvent.

[0118] Understandably, in the face cream, the weight percentage of the composition that improves skin firmness as described above includes, but is not limited to, 0.21 wt%, 1 wt%, 2 wt%, 3 wt%, 4 wt%, 4.21 wt%, 5 wt%, 10 wt%, 12 wt%, 15 wt%, 20 wt%, 25 wt%, 30 wt%, 33 wt%, 35 wt%, 40 wt%, 45 wt%, 50 wt%, or 53 wt%.

[0119] Understandably, in the cream, the thickener may be present in a weight percentage of, but is not limited to, 10 wt%, 12 wt%, 15 wt%, 18 wt%, or 20 wt%.

[0120] Optionally, the thickener is selected from one or more of sodium acrylate / sodium acryloyldimethyl taurate copolymer, xanthan gum, carboxymethyl cellulose, diatomaceous earth, and titanium dioxide. Preferably, the thickener is selected from a mixture of sodium acrylate / sodium acryloyldimethyl taurate copolymer, xanthan gum, carboxymethyl cellulose, diatomaceous earth, and titanium dioxide.

[0121] Understandably, in the face cream, the weight percentage of the chelating agent includes, but is not limited to, 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.15 wt%, or 0.2 wt%.

[0122] Optionally, the chelating agent is selected from one or more of disodium ethylenediaminetetraacetate (EDTA-2Na), sodium ethylenediamine di-o-phenylacetate, and tetrasodium glutamate diacetate. Preferably, the chelating agent is disodium ethylenediaminetetraacetate.

[0123] Understandably, in the face cream, the weight percentage of the skin feel modifier includes, but is not limited to, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.4 wt%, 0.5 wt%, 0.6 wt%, 0.8 wt%, or 1 wt%.

[0124] Optionally, the skin feel modifier is selected from one or more of isononyl isononanoate, isopropyl palmitate, isopropyl myristate, and cyclopentamethoxysiloxane. Preferably, the skin feel modifier is isononyl isononanoate.

[0125] Understandably, in the face cream, the weight percentage of the solubilizer includes, but is not limited to, 0.5 wt%, 0.8 wt%, 1 wt%, 2 wt%, or 3 wt%.

[0126] Optionally, the solubilizer is selected from one or more of polyethylene glycol stearate, coconut oil diethanolamide, and PEG-160 sorbitan triisostearate. Further, the polyethylene glycol has a weight-average molecular weight of 100, and the solubilizer is PEG-100 stearate.

[0127] Understandably, in the face cream, the weight percentage of the solvent includes, but is not limited to, 22.8 wt%, 25 wt%, 30 wt%, 35 wt%, 40 wt%, 45 wt%, 50 wt%, 55 wt%, 60 wt%, 65 wt%, 70 wt%, 75 wt%, 80 wt%, 85.18 wt%, or 89.18 wt%.

[0128] Optionally, the solvent is water.

[0129] In one embodiment, the face cream comprises the following components by weight percentage:

[0130] The composition includes: 0.1wt%~10wt% golden microalgae, 0.1wt%~20wt% rice fermentation broth, 0.01wt%~3wt% inositol, 0.5wt%~5wt% shea butter, 0.5wt%~5wt% jojoba oil, 1wt%~5wt% tocopheryl acetate, 2wt%~5wt% behenol, 2wt%~5wt% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3wt%~5wt% carbomer, 3.5wt%~6wt% carboxymethyl cellulose, 1wt%~2wt% diatomaceous earth, 0.5wt%~2wt% titanium dioxide, 0.01wt%~0.2wt% disodium ethylenediaminetetraacetate, 0.1wt%~1wt% isononyl isononanoate, 0.5wt%~3wt% polyethylene glycol stearate, and 22.8wt%~85.18wt% water.

[0131] Optionally, the face cream may also include one or more of preservatives and fragrances.

[0132] Further, optionally, the preservative is selected from one or both of phenoxyethanol and pentanediol.

[0133] Further optionally, the preservative in the face cream is 0.5wt% to 1.5wt% by weight. This includes, but is not limited to, 0.5wt%, 1wt%, or 1.5wt%. Even further, phenoxyethanol is 0.3wt% to 0.8wt% by weight in the face cream, and pentylene glycol is 0.2wt% to 0.7wt% by weight in the face cream.

[0134] Optionally, the fragrance is a daily-use fragrance.

[0135] In one preferred embodiment, the face cream comprises the following components by weight percentage:

[0136] Golden microalgae 0.1wt%~10wt%, rice fermentation liquid 0.1wt%~20wt%, inositol 0.01wt%~3wt%, shea butter 0.5wt%~5wt%, jojoba oil 0.5wt%~5wt%, tocopheryl acetate 1wt%~5wt%, behenol 2wt%~5wt%, sodium acrylate / sodium acryloyl dimethyl taurate copolymer 2wt%~5wt%, carbomer 3wt%~5wt%, carboxymethyl cellulose 3.5wt%~6wt%, diatomaceous earth 1wt%~2wt%, titanium dioxide 0.5wt%~2wt%, disodium ethylenediaminetetraacetate 0.01wt%~0.2wt%, isononyl isononanoate 0.1wt%~1wt%, polyethylene glycol stearate 0.5wt%~3wt%, preservatives, fragrances and water 22.8wt%~84.675wt%.

[0137] In one preferred embodiment, the face cream comprises the following components by weight percentage:

[0138] Golden microalgae 0.1wt%~10wt%, rice fermentation broth 0.1wt%~20wt%, inositol 0.01wt%~3wt%, shea butter 0.5wt%~5wt%, jojoba oil 0.5wt%~5wt%, tocopheryl acetate 1wt%~5wt%, behenol 2wt%~5wt%, sodium acrylate / sodium acryloyl dimethyl taurate copolymer 2wt%~5wt%, carbomer 3wt%~5wt%, carboxymethyl cellulose 3.5wt%~6wt%. wt%, diatomaceous earth 1wt%~2wt%, titanium dioxide 0.5wt%~2wt%, disodium ethylenediaminetetraacetate 0.01wt%~0.2wt%, isononyl isononanoate 0.1wt%~1wt%, polyethylene glycol stearate 0.5wt%~3wt%, phenoxyethanol 0.3wt%~0.8wt%, pentylene glycol 0.2wt%~0.7wt%, fragrance 0.005wt%~0.1wt%, and water 22.8wt%~84.675wt%.

[0139] The face cream provided by this invention has excellent transdermal and antioxidant properties, which can penetrate deep into the skin to remove free radicals, fundamentally protect the skin from free radical damage, and enhance the production of collagen and elastin fibers, thereby achieving skin firmness and elasticity, and realizing the purpose of deep and multidimensional improvement of skin firmness and elasticity.

[0140] A third aspect of the present invention provides a method for preparing the cosmetic as described above, the technical solution of which is as follows:

[0141] A method for preparing a cosmetic as described above includes the following steps:

[0142] Mix the components together.

[0143] In one embodiment, the cosmetic is a face cream, and its preparation method includes the following steps:

[0144] The chelating agent and a portion of the thickener are mixed in a solvent to prepare a first mixture;

[0145] A second mixture is prepared by combining the first mixture, the remaining thickener, the composition for improving skin firmness, and the skin feel modifier.

[0146] The second mixture and the solubilizer are mixed and emulsified.

[0147] For formulations containing preservatives, the preservatives are mixed with a chelating agent and a portion of a thickener in a solvent to prepare a first mixture.

[0148] For formulations containing fragrance, the second mixture is combined with the solubilizer and fragrance, and then emulsified.

[0149] The following description is further illustrated with specific embodiments and comparative examples. Unless otherwise specified, the raw materials involved in the following specific embodiments and comparative examples are all commercially available. Unless otherwise specified, the instruments used are all commercially available. Unless otherwise specified, the processes involved are conventionally selected by those skilled in the art.

[0150] Examples 1 to 7

[0151] Referring to the weight percentages shown in Table 1, Examples 1 to 7 provide a composition for improving skin firmness, a face cream, and a method for preparing the face cream, the steps of which are as follows:

[0152] 1) Preparation of rice fermentation liquid

[0153] The stock solution of yeast (Saccharomyces veronae) was inoculated into sterilized activation medium and incubated at 28°C for 48 hours for later use. Under aseptic conditions, the activated inoculum was transferred to sterilized expansion medium using an inoculation loop and incubated in a constant temperature shaker at 150 rpm and 28°C for 48 hours. The expanded inoculum was then inoculated into MRS medium at an inoculation rate of 3% and incubated at 28°C for another 48 hours to obtain a yeast suspension.

[0154] Wash 20g of rice with water, soak for 24 hours, drain and steam in a pot until cooked, then put into a bottle, add deionized water and let stand and cool to room temperature, while loosening the rice grains; transfer 15% of the yeast inoculum suspension of cooked rice grains into the cooked rice grains, incubate and ferment at 37℃ for 2 days, then let stand, filter and collect the clear liquid to obtain rice fermentation liquid.

[0155] 2) Preparation of the first mixture

[0156] Phase A was completely wetted and dispersed in water to obtain the first mixture.

[0157] 3) Preparation of the second mixture

[0158] Add phases B, C, and E to the first mixture in step 2), heat to 60°C, and homogenize at 3000 r / min for 3-5 minutes to ensure complete dispersion, thus obtaining the second mixture.

[0159] 4) Prepare face cream

[0160] Add phase D to the second mixture in step 3), homogenize it at 3000 r / min for 3 minutes to ensure complete emulsification, and heat it to 60°C to obtain the face cream.

[0161] Table 1

[0162] .

[0163] Referring to the weight percentages shown in Table 2 and the methods of Examples 1 to 6, Comparative Examples 1 to 10 provide a composition, cream, and lotion for improving skin firmness.

[0164] Table 2

[0165] .

[0166] test

[0167] 1. Detection experiment of type I collagen expression level

[0168] The amount of collagen in the skin is an important indicator of the skin's plumpness and elasticity. Type I collagen can provide strong support for the skin, and an increase in its expression means improved skin elasticity and firmness.

[0169] Healthy fibroblasts were used as experimental subjects, and Examples 1 to 6 and Comparative Examples 1 to 10 were used.

[0170] Cells were seeded into 6-well plates and incubated overnight at 37°C with 5% CO2. When the cell deposition rate reached 40%–60%, 2 ml of sample was applied according to the formulation for each group, with three replicates per group. Cells were then incubated at 37°C with 5% CO2 for 24 hours. The cell culture supernatant was collected into EP tubes and stored at -80°C or immediately for analysis. Detection was performed according to the Collagen I ELISA kit instructions, and statistical analysis was performed using GraphPad Prism software.

[0171] Table 3. Results of Collagen I content detection

[0172] .

[0173] The results showed that, compared to Comparative Example 7 which did not contain the skin-firming composition, the Collagen I content in Examples 1-6, Comparative Examples 1-6, and Comparative Examples 8-10 was increased, and the increase rate was significantly correlated with the composition concentration. This effectively demonstrates that the increase in Collagen I content is composition-dependent, indicating that the composition provided by the present invention has a significant effect on promoting collagen expression in dermal cells, thereby improving skin firmness and elasticity. Furthermore, compared to Comparative Examples 1-10, the Collagen I content in Examples 1-6 was significantly increased, indicating a significant effect on improving skin firmness and elasticity.

[0174] 2. The human efficacy test involved 160 healthy Chinese women with sensitive skin, divided into 16 groups of 10 participants each. Each group used samples from both the implementation and comparative studies. Participants were aged 20 to 45 years, with a mean age of 36.55 years. All participants provided informed consent in writing. Participants agreed to cooperate and understand the necessity and duration of the treatment in order to adhere to the protocol established by the clinical trial center. The product was used twice daily, morning and evening, for 56 consecutive days. Skin firmness and elasticity were measured on days 7, 28, and 56 using a Cutometer® dual MPA580 skin elastometry instrument.

[0175] The calculation method for the rate of change in 2.1 and 2.2 below is as follows: Rate of change = (Average value of each group of measurements after using the product - Average value of each group of measurements before using the product) ÷ Average value of each group of measurements before using the product × 100%.

[0176] 2.1 Skin Tightness Test

[0177] The Cutometer® dual MPA580 skin elasticity meter is used to test skin firmness. The test environment conditions (temperature 20℃~22℃, humidity 40%~60%) are set according to the test requirements. Subjects should acclimatize to the test environment for at least 15~30 minutes before the test. A lower F4 skin firmness measurement indicates improved skin firmness.

[0178] Table 4. Change rate (%) of skin firmness F4 measurement values

[0179] .

[0180] 2.2 Changes in skin elasticity R7 value

[0181] The Cutometer® dual MPA580 skin elasticity meter is used to measure skin elasticity. A higher skin elasticity R7 value indicates improved skin elasticity.

[0182] Table 5. Change rate of skin elasticity R7 value (%)

[0183] .

[0184] As shown in Tables 4 and 5, compared with before use, after 7, 28, and 56 days of use of Example 1, the skin firmness F4 value decreased significantly, and the skin elasticity R7 value increased significantly. This indicates that the formula described in Example 1 can effectively improve skin firmness and elasticity, and can continue to have an anti-aging effect with prolonged use. The absolute values ​​of the change rates of all indicators in Example 2 were greater than those in Examples 1 and 3, and the absolute values ​​of the change rates of all indicators in Example 3 were lower than those in Example 1. This indicates that within this range, the anti-aging effect of the composition increases with increasing concentration, suggesting that the anti-aging effect of this product is dependent on the composition.

[0185] The changes in each indicator in Comparative Examples 1 to 10 were less pronounced than in Example 1. Comparative Example 7 showed no significant effect in altering the skin firmness F4 and skin elasticity R7 values, indicating that the components other than the skin firmness-enhancing composition are good basic ingredients. This further proves that the skin firmness and elasticity-enhancing effects originate from the composition of this invention. The examples contained yeast / rice fermentation product filtrate, golden microalgae, and inositol, while Comparative Examples 1 to 6 contained only two or one of these three components. Comparative Example 7 did not contain the skin firmness-enhancing composition. Comparative Example 8 used brown algae extract instead of the golden microalgae in this invention, Comparative Example 9 used adenosine instead of the rice fermentation liquid in this invention, and Comparative Example 10 used panthenol instead of inositol. The results showed that Example 1 was superior to the effects of the comparative examples, thus demonstrating that rice fermentation liquid, golden microalgae, and inositol have a synergistic effect.

[0186] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0187] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A face cream, characterized in that, Includes the following components by weight percentage: The ingredients are: golden microalgae 1wt%~10wt%, rice fermentation liquid 1.5wt%~20wt%, inositol 0.5wt%~3wt%, shea butter 0.5wt%~5wt%, jojoba oil 0.5wt%~5wt%, tocopheryl acetate 1wt%~5wt%, behenol 2wt%~5wt%, sodium acrylate / sodium acryloyl dimethyl taurate copolymer 2wt%~5wt%, carbomer 3wt%~5wt%, carboxymethyl cellulose 3.5wt%~6wt%, diatomaceous earth 1wt%~2wt%, titanium dioxide 0.5wt%~2wt%, disodium ethylenediaminetetraacetate 0.01wt%~0.2wt%, isononyl isononanoate 0.1wt%~1wt%, polyethylene glycol stearate 0.5wt%~3wt%, and water 22.8wt%~85.18wt%. The fermentation strain of the rice fermentation liquid is yeast; The gold content in gold microalgae ranges from 2 mg / L to 10 mg / L.

2. The face cream according to claim 1, characterized in that, The method for preparing the rice fermentation liquid includes the following steps: Yeast stock solution and culture medium are mixed and cultured to prepare yeast strain suspension; The yeast strain suspension is inoculated into cooked rice grains, and fermentation is carried out to prepare the rice fermentation broth.

3. The face cream according to claim 2, characterized in that, The preparation parameters of the rice fermentation liquid meet one or more of the following conditions (1) to (4): (1) The yeast strain is Saccharomyces veronae; (2) The inoculation ratio of the yeast strain suspension and the cooked rice grains is 10wt%~20wt%; (3) The fermentation culture temperature is 30℃~40℃; (4) The fermentation time is 10h~72h.

4. A method for preparing a face cream according to any one of claims 1 to 3, characterized in that, Includes the following steps: Mix the components together.