A method for purifying effective ingredients in Bletilla striata extract

Abstract

The invention is applicable to the technical field of bletilla striata extraction and provides a purification method for effective components in bletilla striata extract. The method comprises the following steps: adding bletilla striata powder into a circulation extraction system, independently adding hot water into the circulation extraction system in 5-10 times; using the circulation extraction system to mobilize hot water to continuously circulate and rinse the bletilla striata powder, draining it out and performing the next heating water, and repeating the above steps; taking out the bletilla striata powder extracted from the circulation extraction system, adding a bletilla striata crude extract and a clarifier into the circulation extraction system, and performing circulation heating and mixing; cooling the mixture and performing a centrifugal operation to collect a supernatant and concentrate it; re-adding the mixture into the circulation extraction system together with ethanol, circulating and mixing, taking out a precipitate and vacuum drying it to obtain bletilla striata polysaccharide; the hot water can fully contact with the bletilla striata powder, completing a single high-efficiency crude extraction, and repeating the single high-efficiency crude extraction to realize calculus-type high-efficiency crude extraction of the bletilla striata polysaccharide in the bletilla striata powder.

Description

Technical Field

[0001] The present invention relates to the technical field of bletilla striata extraction, and more particularly to a method for purifying effective components in bletilla striata extract. Background Art

[0002] Bletilla striata, also known as white twig, is a species of the genus Bletilla in the Orchidaceae family; its tuber extract is mainly Bletilla striata polysaccharide; Bletilla striata polysaccharide is a common medicinal polysaccharide with a wide range of biological activities and pharmacological effects. It is extracted from the traditional Chinese medicine Bletilla striata, has multiple health care and therapeutic effects, and is widely used in the field of traditional Chinese medicine; Bletilla striata polysaccharide is mainly composed of glucose and xylose, and has multiple functional groups, such as hydroxyl, methyl and oxygen groups, which give Bletilla striata polysaccharide special chemical properties and pharmacological activities.

[0003] In the current environment of abuse of chemical additives, the rational application of Chinese herbal medicine extracts is a greener and healthier approach. Bletilla striata polysaccharide, which is an extract of Bletilla striata, is one of the greener and healthier applications.

[0004] The seeds of Bletilla striata are extremely small, like powder, without endosperm, and only have incompletely developed embryos composed of several cells. Through tissue culture technology, a large number of seedlings can be rapidly propagated. They are sown aseptically on different culture media, and after the seeds germinate, they are propagated asexually using tissue culture methods.

[0005] Bletilla striata polysaccharide has multiple health benefits, including antioxidant, anti-inflammatory, anti-tumor, and immunomodulatory properties. Its antioxidant properties enable it to scavenge free radicals in the body and mitigate damage caused by oxidative stress. Its anti-inflammatory properties reduce inflammatory responses and provide analgesia. Its anti-tumor effects make it important in the prevention and treatment of tumors. Its immunomodulatory properties enhance the body's immune function and improve disease resistance. Bletilla striata polysaccharide also possesses multiple therapeutic effects. It is widely used in Traditional Chinese Medicine to treat a variety of conditions, including cardiovascular and cerebrovascular diseases, tumors, chronic inflammation, and immune system disorders. Bletilla striata polysaccharide modulates related signaling pathways, regulating cell proliferation and apoptosis, and inhibiting tumor growth and spread. It also regulates immune system function, enhancing the body's resistance and preventing and treating disease.

[0006] Extraction methods for Bletilla striata extract include water decoction, enzymatic extraction, ultrasonic extraction, ion exchange resin, and graded precipitation. Many of these extraction methods involve direct soaking of Bletilla striata with the auxiliary extraction solvent. To improve the mixing degree and extraction rate of Bletilla striata and the auxiliary extraction solvent, the prior art incorporates stirring and mixing mechanisms. For example, the Chinese patent "A method for preparing Bletilla striata extract using ultrasonic technology" (Granted Publication No. CN106866835 B) states that "an ethanol-ammonium sulfate two-phase aqueous system is used as an extractant, the raw material powder and the extractant are mixed at a certain solid-liquid ratio, stirred evenly, and then ultrasonically extracted." However, stirring can also result in dead zones, affecting the purity and extraction rate of Bletilla striata extract. That is, the contact and mixing of Bletilla striata and the auxiliary extraction solvent is a key factor in determining the purity and extraction rate of Bletilla striata extract.

[0007] Therefore, in view of the above problems, a method for purifying the effective components in Bletilla striata extract is proposed. Summary of the Invention

[0008] Technical problem. The purpose of the present invention is to solve the shortcomings existing in the prior art: many extraction methods of Bletilla striata use related auxiliary extraction solvents that are directly soaked with Bletilla striata. In order to improve the mixing degree and extraction rate of Bletilla striata and related auxiliary extraction solvents, the prior art will add some stirring and mixing mechanisms, but there will also be dead corners in the stirring, which will affect the extraction purity and extraction rate of Bletilla striata extract. That is, the contact and mixing of Bletilla striata and related auxiliary extraction solvents is one of the key factors that determine the extraction purity and extraction rate of Bletilla striata extract. A purification method for purifying the effective ingredients in Bletilla striata extract is proposed.

[0009] The specific technical solution is: a method for purifying the active ingredients in the Bletilla striata extract, comprising the following steps:

[0010] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0011] Step 2: 15-25 parts by weight of Bletilla striata powder is added to a circulating extraction system, and 350-450 parts by weight of hot water at a temperature of 90-100 degrees is added to the circulating extraction system independently in 5-10 times; after each addition of hot water, the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1-1.5 hours to produce a single crude extract, which is then drained and heated for the next time, and the above operation is repeated; after 5-10 repetitions, all the single crude extracts are added together to produce a Bletilla striata crude extract;

[0012] During each single crude extraction process of adding hot water, the circulating extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.5-1.2 hours;

[0013] Hot water is added to the circulating extraction system independently multiple times to form multiple independent single crude extractions; during the single crude extraction process, the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder, so that the hot water and the Bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of Bletilla striata polysaccharides inside the Bletilla striata powder; at the same time, ultrasound has a disruptive effect on cell tissue, which helps to increase the leaching rate of Bletilla striata polysaccharides in hot water and extract them into hot water, thereby achieving efficient crude extraction and subsequent Bletilla striata extract purity;

[0014] Step 3: Take out the extracted Bletilla striata powder from the circulation extraction system, then add the crude Bletilla striata extract and the clarifier into the circulation extraction system in a volume ratio of 1-3:7-10, and carry out circulation heating and mixing for 2-3 hours; then cool to 10-20 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulation extraction system together with ethanol, carry out circulation mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0015] The technical solution of this application is further described below:

[0016] In one embodiment, in step 2, 18 parts by weight of Bletilla striata powder are added to the circulation extraction system, and 400 parts by weight of hot water at 92 degrees Celsius are added to the circulation extraction system independently in 8 times; after each addition of hot water, the circulation extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1.2 hours to produce a single crude extract, which is then drained and heated for the next time, and the above operation is repeated; after 8 repetitions, all the single crude extracts are added together to produce a Bletilla striata crude extract; during each addition of hot water for a single crude extraction, the circulation extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.8 hours.

[0017] Hot water at 92 degrees Celsius is added to the circulating extraction system independently 8 times, forming multiple independent single crude extractions lasting 1.2 hours. During the single crude extraction, the circulating extraction system is used to mobilize hot water to continuously circulate and flush the Bletilla striata powder, so that the hot water and the Bletilla striata powder are fully in contact, completing a single efficient crude extraction. Repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of Bletilla striata polysaccharides inside the Bletilla striata powder. At the same time, the 0.8-hour ultrasonic wave has a fragmenting effect on cell tissue, which helps to increase the extraction rate of Bletilla striata polysaccharides in hot water and extract them into hot water, achieving efficient crude extraction.

[0018] In one embodiment, in step three, the extracted Bletilla striata powder in the circulation extraction system is taken out, and then the crude Bletilla striata extract and the clarifier are added into the circulation extraction system in a volume ratio of 2:9, and circulated heating and mixing are carried out for 2.3 hours; after cooling to 15 degrees, centrifugation is performed, and the supernatant is concentrated; after concentration, it is re-added into the circulation extraction system together with ethanol, and circulated mixing is carried out. Finally, after being taken out and allowed to stand, the precipitate is taken out and vacuum dried to obtain Bletilla striata polysaccharide.

[0019] In the technical solution of the present invention, the circulating extraction system includes a circulating heating assembly and a circulating extraction pipe assembly, and both ends of the circulating heating assembly are respectively wound around and connected to both ends of the circulating extraction pipe assembly to form a closed loop.

[0020] In a further technical solution, the circulating heating assembly includes a heater with a pump installed inside the heater; a circulating pipe is connected to each end of the heater, and the circulating pipe includes a circulating feed pipe and a circulating discharge pipe. The circulating feed pipe is fixed with a connector at the end away from the heater, and the circulating discharge pipe is fixed with a liquid extraction pan at the end away from the heater.

[0021] In the optimized technical solution, the connector is equipped with a mounting flange at the connection with the circulating extraction pipe assembly; the liquid extraction plate includes a positioning plate and a connecting pipe, a cavity is provided inside the positioning plate, and the cavity is connected to the circulating discharge pipe; a plurality of connecting pipes are provided and distributed in a circular array on the positioning plate; the connecting pipe is connected to the cavity.

[0022] In a further technical solution, the circulating extraction pipe assembly includes a circulating extraction pipe and a unit extrusion extraction piece; a plurality of unit extrusion extraction pieces are provided and arranged in a ring array around the circulating extraction pipe and extending into the interior of the circulating extraction pipe.

[0023] In the optimized technical solution, a unit extraction chamber is divided inside the circulating extraction tube inside the unit extrusion extraction component, and a water outlet head is provided at the end of the unit extraction chamber, which is connected to the circulating heating assembly; a plurality of water outlet holes are provided on the water outlet head, and filter cloth A is installed inside the water outlet holes; a water permeable plate is provided inside the unit extraction chamber, and a liquid inlet hole is provided on the circulating extraction pipe column inside the water permeable plate toward the center line; the liquid inlet hole is connected to the circulating heating assembly; a plurality of water inlet holes distributed in an array are provided on the water permeable plate, and filter cloth B is installed inside the water inlet hole.

[0024] The unit extrusion extraction component includes a storage box, which is integrally fixed on the circulation extraction tube. The storage box is fixed with a storage tube. An electric telescopic rod is inserted and fixed inside the storage tube. The electric telescopic rod is fixed with an extrusion plate at its end. The electric telescopic rod is telescopically adjusted to drive the extrusion plate to stir and extrude inside the unit extraction chamber.

[0025] After the bletilla striata powder is added into the unit extraction chamber, hot water is introduced. The hot water is dispersed and enters through multiple water inlet holes on the permeable plate, and evenly rushes towards the bletilla striata powder and mixes with it. The electric telescopic rod is started to be telescopically adjusted to drive the extrusion plate to stir and extrude the bletilla striata powder inside the unit extraction chamber. On the one hand, it promotes the extraction of the bletilla striata powder by hot water, and on the other hand, it promotes the contact between the bletilla striata powder and the hot water. Thereafter, the hot water after the bletilla striata powder is extracted enters the circulating discharge pipe through the water outlet head. The circulating discharge pipe guides the hot water into the heater, and then enters the liquid inlet hole through the circulating feed pipe, and finally enters the unit extraction chamber again through the permeable plate.

[0026] The extrusion plate includes an outer receiving plate, and an inner interpenetrating partition plate is inserted on both sides of the outer receiving plate respectively. The inner interpenetrating partition plate is elastically connected to the outer receiving plate through a plurality of springs.

[0027] Compared with the prior art, the method for purifying the active ingredients in the Bletilla striata extract of the present invention can achieve the following:

[0028] 1. Hot water is added to the circulating extraction system independently for multiple times, forming multiple independent single crude extractions; during the single crude extraction process, the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder, so that the hot water and the Bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of Bletilla striata polysaccharides inside the Bletilla striata powder; at the same time, ultrasound has a disruptive effect on cell tissue, which helps to increase the leaching rate of Bletilla striata polysaccharides in hot water and extract them into the hot water, thereby achieving efficient crude extraction;

[0029] 2. After the bletilla striata powder is added to the unit extraction chamber, hot water is introduced. The hot water is dispersed and enters through multiple water inlet holes on the permeable plate, and evenly rushes towards the bletilla striata powder and mixes with it. The electric telescopic rod is started to be telescopically adjusted to drive the extrusion plate to stir and extrude the bletilla striata powder inside the unit extraction chamber. On the one hand, it promotes the extraction of the bletilla striata powder by hot water, and on the other hand, it promotes the contact between the bletilla striata powder and the hot water. Thereafter, the hot water after the bletilla striata powder is extracted enters the circulating discharge pipe through the water outlet head. The circulating discharge pipe guides the hot water into the heater, and then enters the liquid inlet hole through the circulating feed pipe, and finally enters the unit extraction chamber again through the permeable plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0031] Figure 1 This is a schematic structural diagram of a circulating extraction system used in a method for purifying active ingredients in a Bletilla striata extract according to one embodiment of the present invention;

[0032] Figure 2 for Figure 1 Schematic diagram of the structure of the medium-circulation heating assembly;

[0033] Figure 3 for Figure 1 Schematic diagram of the structure of the intermediate circulation extraction pipe assembly;

[0034] Figure 4 for Figure 3 A cross-sectional view of the intermediate circulation extraction pipe assembly;

[0035] Figure 5 for Figure 4 Schematic diagram of the structure of the middle unit extrusion extraction component;

[0036] Figure 6 for Figure 5 Cross-sectional view of the extrusion extraction component in the middle unit.

[0037] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0038] 100, circulating heating assembly; 110, connector, 120, heater, 130, circulating pipe, 140, liquid extraction tray; 1101, mounting flange; 1401, positioning plate, 1402, connecting pipe;

[0039] 200. Circulating extraction tube assembly; 210. Circulating extraction tube, 220. Unit extrusion extraction member, 230. Liquid inlet, 240. Unit extraction chamber, 250. Water permeable plate, 260. Water outlet; 2201. Storage tube, 2202. Storage box, 2203. Extrusion plate, 2204. Electric telescopic rod; 22031. Internal interlaced partition, 22032. External storage plate. DETAILED DESCRIPTION

[0040] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] A method for purifying effective ingredients in a Bletilla striata extract comprises the following steps:

[0043] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0044] Step 2: 15 parts by weight of Bletilla striata powder is added to the inside of the circulation extraction system, and 350 parts by weight of hot water at a temperature of 90 degrees is added to the inside of the circulation extraction system independently in 5 times; after each addition of hot water, the circulation extraction system is used to mobilize the hot water to continuously circulate and rinse the Bletilla striata powder for 1 hour to prepare a single crude extract, which is then drained and heated for the next time, and the above operation is repeated; after 5 repetitions, all the single crude extracts are added together to prepare a Bletilla striata crude extract;

[0045] During each single crude extraction process of adding hot water, the circulating extraction system where the Bletilla striata powder was stored was ultrasonicated for 0.5 h;

[0046] Summarizing the above introduction, it can be concluded that: hot water is added to the circulating extraction system independently for multiple times to form multiple independent single crude extractions; during the single crude extraction process, the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the bletilla striata powder, so that the hot water and the bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of bletilla striata polysaccharides inside the bletilla striata powder; at the same time, ultrasound has a disruptive effect on cell tissue, which helps to increase the leaching rate of bletilla striata polysaccharides in hot water and extract them into hot water, thereby achieving efficient crude extraction and subsequent bletilla striata extract purity;

[0047] Step 3: Take out the extracted Bletilla striata powder from the circulating extraction system, then add the crude Bletilla striata extract and the clarifier into the circulating extraction system at a volume ratio of 1:7, and carry out circulating heating and mixing for 2 hours; then cool to 10 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulating extraction system together with ethanol, carry out circulating mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0048] Example 2

[0049] A method for purifying effective ingredients in a Bletilla striata extract comprises the following steps:

[0050] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0051] Step 2: Add 25 parts by weight of Bletilla striata powder to the inside of a circulation extraction system, and add 450 parts by weight of hot water at a temperature of 100 degrees Celsius to the inside of the circulation extraction system independently in 10 times; after each addition of hot water, use the circulation extraction system to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1.5 hours to prepare a single crude extract, then drain it, and heat water for the next time, and repeat the above operation; after 10 repetitions, all the single crude extracts are added together to prepare a Bletilla striata crude extract;

[0052] During each single crude extraction process of adding hot water, the circulating extraction system where the Bletilla striata powder was stored was ultrasonicated for 1.2 hours;

[0053] Summarizing the above introduction, it can be concluded that: hot water is added to the circulating extraction system independently for multiple times to form multiple independent single crude extractions; during the single crude extraction process, the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the bletilla striata powder, so that the hot water and the bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of bletilla striata polysaccharides inside the bletilla striata powder; at the same time, ultrasound has a disruptive effect on cell tissue, which helps to increase the leaching rate of bletilla striata polysaccharides in hot water and extract them into hot water, thereby achieving efficient crude extraction and subsequent bletilla striata extract purity;

[0054] Step 3: Take out the extracted Bletilla striata powder from the circulation extraction system, then add the crude Bletilla striata extract and the clarifier into the circulation extraction system in a volume ratio of 3:10, and carry out circulation heating and mixing for 3 hours; then cool to 20 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulation extraction system together with ethanol, carry out circulation mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0055] Example 3

[0056] A method for purifying effective ingredients in a Bletilla striata extract comprises the following steps:

[0057] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0058] Step 2: 18 parts by weight of Bletilla striata powder are added to the inside of the circulation extraction system, and 400 parts by weight of hot water at a temperature of 92 degrees are added to the inside of the circulation extraction system independently in 8 times; after each addition of hot water, the circulation extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1.2 hours to prepare a single crude extract, which is then drained out and heated for the next time, and the above operation is repeated; after 8 repetitions, all the single crude extracts are added together to prepare a Bletilla striata crude extract; during each addition of hot water for a single crude extraction, the circulation extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.8 hours.

[0059] Summarizing the above introduction, it can be concluded that: hot water at 92 degrees is added independently into the circulating extraction system 8 times, forming multiple independent single crude extractions lasting 1.2 hours; the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the bletilla striata powder during the single crude extraction process, so that the hot water and the bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of bletilla striata polysaccharides inside the bletilla striata powder; at the same time, the ultrasonic wave for 0.8 hours has a crushing effect on cell tissue, which helps to increase the leaching rate of bletilla striata polysaccharides in hot water and extract them into hot water, thereby achieving efficient crude extraction;

[0060] Step 3: Take out the extracted Bletilla striata powder from the circulation extraction system, then add the crude Bletilla striata extract and the clarifier into the circulation extraction system in a volume ratio of 3:10, and carry out circulation heating and mixing for 3 hours; then cool to 20 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulation extraction system together with ethanol, carry out circulation mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0061] Example 4

[0062] A method for purifying effective ingredients in a Bletilla striata extract comprises the following steps:

[0063] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0064] Step 2: 18 parts by weight of Bletilla striata powder are added to the inside of the circulation extraction system, and 400 parts by weight of hot water at a temperature of 92 degrees are added to the inside of the circulation extraction system independently in 8 times; after each addition of hot water, the circulation extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1.2 hours to prepare a single crude extract, which is then drained and heated for the next time, and the above operation is repeated; after 8 repetitions, all the single crude extracts are added together to prepare a Bletilla striata crude extract; during each addition of hot water for a single crude extraction process, the circulation extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.8 hours;

[0065] Summarizing the above introduction, it can be concluded that: hot water at 92 degrees is added independently into the circulating extraction system 8 times, forming multiple independent single crude extractions lasting 1.2 hours; the circulating extraction system is used to mobilize hot water to continuously circulate and rinse the bletilla striata powder during the single crude extraction process, so that the hot water and the bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of bletilla striata polysaccharides inside the bletilla striata powder; at the same time, the ultrasonic wave for 0.8 hours has a crushing effect on cell tissue, which helps to increase the leaching rate of bletilla striata polysaccharides in hot water and extract them into hot water, thereby achieving efficient crude extraction;

[0066] Step 3: Take out the extracted Bletilla striata powder from the circulating extraction system, then add the crude Bletilla striata extract and the clarifier into the circulating extraction system in a volume ratio of 2:9, and carry out circulating heating and mixing for 2.3 hours; then cool to 15 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulating extraction system together with ethanol, carry out circulating mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0067] Example 5

[0068] The only difference between this embodiment and embodiment 4 is that:

[0069] like Figure 1 As shown: the circulating extraction system includes a circulating heating assembly 100 and a circulating extraction pipe assembly 200, and both ends of the circulating heating assembly 100 are respectively connected to both ends of the circulating extraction pipe assembly 200 to form a closed loop.

[0070] like Figure 1 and Figure 2As shown: the circulating heating assembly 100 includes a heater 120, and a pump is installed inside the heater 120; a circulating pipe 130 is connected to each end of the heater 120, and the circulating pipe 130 includes a circulating feed pipe and a circulating discharge pipe, and the circulating feed pipe is fixed with a connector 110 at the end away from the heater 120, and the circulating discharge pipe is fixed with a liquid extraction pan 140 at the end away from the heater 120.

[0071] It should be noted that the heater 120 is a prior art, and its detailed structure can be found in existing literature journals. It can also be purchased directly on the market, or its components can be purchased on the market to assemble it, etc. It is not protected by the present invention, so it will not be elaborated here, nor drawn in the accompanying drawings.

[0072] like Figure 2 As shown: the connector 110 is installed with a mounting flange 1101 at the connection with the circulating extraction pipe assembly 200; the liquid extraction plate 140 includes a positioning plate 1401 and a connecting pipe 1402, the positioning plate 1401 has a cavity inside, and the cavity is connected to the circulating discharge pipe; a plurality of connecting pipes 1402 are provided, and are distributed in a circular array on the positioning plate 1401; the connecting pipe 1402 is connected to the cavity.

[0073] Example 6

[0074] The only difference between this embodiment and embodiment 5 is that:

[0075] like Figure 3 and Figure 4 As shown, the circulating extraction pipe assembly 200 includes a circulating extraction pipe 210 and a unit extrusion extraction member 220 ; a plurality of unit extrusion extraction members 220 are provided in a ring array around the circulating extraction pipe 210 and extend into the interior of the circulating extraction pipe 210 .

[0076] It should be noted that the feeding structure on the circulating extraction pipe 210, such as the feeding port, feeding pipe, etc., are all existing technologies. Their detailed structures can be found in existing literature journals, and can also be purchased directly on the market, or parts can be purchased on the market to assemble them, etc.; they are not protected by the present invention, and will not be elaborated here, nor drawn in the accompanying drawings.

[0077] like Figure 3-6 As shown: the inside of the circulating extraction pipe 210 inside the unit extrusion extraction member 220 is divided into a unit extraction chamber 240, and the unit extraction chamber 240 is provided with a water outlet head 260 at its end, and the water outlet head 260 is connected to the circulating heating assembly 100 (specifically, the water outlet head 260 is connected to the circulating discharge pipe);

[0078] The water outlet head 260 is provided with a plurality of water outlet holes, and filter cloth A is installed inside the water outlet holes;

[0079] A water-permeable plate 250 is provided on the inner side of the unit extraction chamber 240, and a liquid inlet hole 230 is provided on the center line of the circulation extraction pipe 210 column inside the water-permeable plate 250; the liquid inlet hole 230 is connected to the circulation heating assembly 100 (specifically, the water outlet head 260 is connected to the circulation feed pipe); a plurality of water inlet holes distributed in an array are provided on the water-permeable plate 250, and a filter cloth B is installed inside the water inlet hole.

[0080] like Figure 3-6 As shown: the unit extrusion extraction member 220 includes a storage box 2202, which is integrally fixed to the circulation extraction pipe 210. A storage tube 2201 is fixed to the storage box 2202. An electric telescopic rod 2204 is inserted and fixed inside the storage tube 2201. The electric telescopic rod 2204 has an extrusion plate 2203 fixed at its end.

[0081] The electric telescopic rod 2204 is telescopically adjusted to drive the squeezing plate 2203 to stir and squeeze inside the unit extraction chamber 240 .

[0082] To summarize the above introduction, it can be concluded that: after the bletilla striata powder is added to the unit extraction chamber 240, hot water is introduced, and the hot water is dispersed and entered through the multiple water inlet holes on the permeable plate 250, and evenly rushes towards the bletilla striata powder and mixes with it. The electric telescopic rod 2204 is started to telescope and adjust, so as to drive the extrusion plate 2203 to stir and squeeze the bletilla striata powder inside the unit extraction chamber 240, on the one hand promoting the extraction of the bletilla striata powder by hot water, and on the other hand promoting the contact between the bletilla striata powder and the hot water; thereafter, the hot water after the bletilla striata powder is extracted enters the circulating discharge pipe through the water outlet head 260, and the circulating discharge pipe introduces the hot water into the heater 120, and then enters the liquid inlet hole 230 through the circulating feed pipe, and finally enters the unit extraction chamber 240 again through the permeable plate 250.

[0083] like Figure 6 As shown: the extrusion plate 2203 includes an outer receiving plate 22032, and an inner interlaced partition plate 22031 is inserted on both sides of the outer receiving plate 22032, and the inner interlaced partition plate 22031 is elastically connected to the outer receiving plate 22032 through multiple springs.

[0084] Comparative Example 1

[0085] The only difference between this embodiment and embodiment 4 is that the circulating extraction system is not used; the details are as follows:

[0086] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0087] Step 2: 18 parts by weight of Bletilla striata powder and 400 parts by weight of hot water at 92 degrees Celsius are added together, stirred and mixed, and ultrasonicated for 0.8 hours to prepare a crude Bletilla striata extract;

[0088] Step 3: Add the crude extract of Bletilla striata and the clarifier in a volume ratio of 2:9, and perform cyclic heating and mixing for 2.3 hours; then cool to 15 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it again together with ethanol, perform cyclic mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0089] Comparative Example 2

[0090] The only difference between this embodiment and embodiment 4 is that the circulating extraction system and ultrasound are not used; the details are as follows:

[0091] Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed;

[0092] Step 2: 18 parts by weight of Bletilla striata powder and 400 parts by weight of hot water at 92 degrees Celsius are added together, stirred and mixed to prepare a crude Bletilla striata extract;

[0093] Step 3: Add the crude extract of Bletilla striata and the clarifier in a volume ratio of 2:9, and perform cyclic heating and mixing for 2.3 hours; then cool to 15 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it again together with ethanol, perform cyclic mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

[0094] The extraction rate and purity of the polysaccharide from Bletilla striata in Examples 1-4 and Comparative Examples 1 and 2 were measured and calculated. The results are shown in Table 1.

[0095] Table 1

[0096] Extraction rate of Bletilla striata polysaccharide Purity of Bletilla striata polysaccharide Example 1 36.11 85.32 Example 2 36.05 83.58 Example 3 36.15 89.21 Example 4 36.30 90.18 Comparative Example 1 32.08 78.02 Comparative Example 2 33.58 77.92

[0097] As can be seen from Table 1, 92-degree hot water was added to the circulating extraction system independently 8 times, forming multiple independent single crude extractions lasting 1.2 hours. During the single crude extraction, the circulating extraction system was used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder, so that the hot water and the Bletilla striata powder were fully in contact, completing a single efficient crude extraction. Repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of Bletilla striata polysaccharides inside the Bletilla striata powder. At the same time, the 0.8-hour ultrasonic wave has a fragmentation effect on cell tissue, which helps to increase the leaching rate of Bletilla striata polysaccharides in hot water and extract them into hot water, achieving efficient crude extraction.

[0098] Many extraction methods for Bletilla striata involve direct soaking of the Bletilla striata with the auxiliary extraction solvents. To improve the mixing degree and extraction rate of Bletilla striata and the auxiliary extraction solvents, the prior art adds some stirring and mixing mechanisms. However, there are also dead spots in the stirring, which affects the extraction purity and extraction rate of the Bletilla striata extract. That is, the contact and mixing of Bletilla striata and the auxiliary extraction solvents is one of the key factors that determine the extraction purity and extraction rate of the Bletilla striata extract. The purification method of the active ingredient in the Bletilla striata extract of the present invention can achieve the following:

[0099] Hot water is added to the circulating extraction system independently in multiple times to form multiple independent single crude extractions; during the single crude extraction process, the circulating extraction system is used to mobilize hot water to continuously circulate and flush the bletilla striata powder, so that the hot water and the bletilla striata powder are fully in contact, completing a single efficient crude extraction, and repeating the single efficient crude extraction can achieve calculus-type efficient crude extraction of bletilla striata polysaccharides inside the bletilla striata powder; at the same time, ultrasound has a crushing effect on cell tissue, which helps to increase the leaching rate of bletilla striata polysaccharides in hot water and extract them into hot water, achieving efficient crude extraction.

[0100] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A circulating extraction system for purifying active ingredients in Bletilla striata extract, characterized in that: It includes a circulating heating assembly and a circulating extraction pipe assembly, with both ends of the circulating heating assembly respectively connected to both ends of the circulating extraction pipe assembly to form a closed loop; The circulating heating assembly includes a heater with a pump installed inside the heater; a circulating pipe is connected to each end of the heater, and the circulating pipe includes a circulating feed pipe and a circulating discharge pipe. The circulating feed pipe is fixed with a connector at the end away from the heater, and the circulating discharge pipe is fixed with a liquid extraction pan at the end away from the heater; the connector is installed with a mounting flange at the connection with the circulating extraction pipe assembly; the liquid extraction pan includes a positioning pan and a connecting pipe, the positioning pan has a cavity formed inside, and the cavity is connected to the circulating discharge pipe; a plurality of connecting pipes are provided and distributed in an annular array on the positioning pan; the connecting pipe is connected to the cavity; The circulating extraction pipe assembly includes a circulating extraction pipe and a unit extrusion extraction member; a plurality of unit extrusion extraction members are provided in a ring array around the circulating extraction pipe and extend into the interior of the circulating extraction pipe; The circulating extraction pipe inside the unit extrusion extraction member is divided into a unit extraction chamber, and the unit extraction chamber is provided with a water outlet head at its end, which is connected to the circulating heating assembly; the water outlet head is provided with multiple water outlet holes, and filter cloth A is installed inside the water outlet holes; a water permeable plate is provided inside the unit extraction chamber, and the circulating extraction pipe column inside the water permeable plate is provided with a liquid inlet hole toward the center line; the liquid inlet hole is connected to the circulating heating assembly; the water permeable plate is provided with multiple water inlet holes distributed in an array, and filter cloth B is installed inside the water inlet holes; The unit extrusion extraction component includes a storage box, which is integrally fixed on the circulation extraction tube. The storage box is fixed with a storage tube. An electric telescopic rod is inserted and fixed inside the storage tube. The electric telescopic rod is fixed with an extrusion plate at its end. The electric telescopic rod is telescopically adjusted to drive the extrusion plate to stir and extrude inside the unit extraction chamber.

2. The circulating extraction system for purifying the active ingredients in the Bletilla striata extract according to claim 1, characterized in that: The extrusion plate includes an outer receiving plate, and an inner interpenetrating partition plate is inserted on both sides of the outer receiving plate respectively. The inner interpenetrating partition plate is elastically connected to the outer receiving plate through a plurality of springs.

3. A method for purifying the active ingredients in a Bletilla striata extract, comprising the following steps: Step 1: After cleaning the white hyacinth with deionized water, crush it to make white hyacinth fragments; air-dry the white hyacinth fragments, and grind them into white hyacinth powder after the air-drying is completed; Step 2: adding 15-25 parts by weight of Bletilla striata powder to the circulation extraction system of claim 1 or 2, and independently adding 350-450 parts by weight of hot water at a temperature of 90-100 degrees into the circulation extraction system in 5-10 times; after each addition of hot water, using the circulation extraction system to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1-1.5 hours to produce a single crude extract, which is then drained and heated for the next time, and the above operation is repeated; after 5-10 repetitions, all the single crude extracts are added together to produce a Bletilla striata crude extract; During each single crude extraction process of adding hot water, the circulating extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.5-1.2 hours; Step 3: Take out the extracted Bletilla striata powder from the circulation extraction system, then add the crude Bletilla striata extract and the clarifier into the circulation extraction system in a volume ratio of 1-3:7-10, and carry out circulation heating and mixing for 2-3 hours; then cool to 10-20 degrees, perform centrifugation, and take the supernatant for concentration; after concentration, add it back into the circulation extraction system together with ethanol, carry out circulation mixing, and finally take it out and let it stand, then take out the precipitate and vacuum dry it to obtain Bletilla striata polysaccharide.

4. The method for purifying the active ingredients in the Bletilla striata extract according to claim 3, wherein: In the step 2, 18 parts by weight of Bletilla striata powder are added to the inside of the circulation extraction system, and 400 parts by weight of hot water at a temperature of 92 degrees are independently added to the inside of the circulation extraction system in 8 times; after each addition of hot water, the circulation extraction system is used to mobilize hot water to continuously circulate and rinse the Bletilla striata powder for 1.2 hours to prepare a single crude extract, which is then drained out and the next heating water is carried out, and the above operation is repeated; after 8 repetitions, all the single crude extracts are added together to prepare a Bletilla striata crude extract; during each single crude extraction process of adding hot water, the circulation extraction system where the Bletilla striata powder is stored is ultrasonicated for 0.8 hours.

5. The method for purifying the active ingredients in the Bletilla striata extract according to claim 3, characterized in that: In the step three, the bletilla striata powder extracted from the circulating extraction system is taken out, and then the crude bletilla striata extract and the clarifier are added into the circulating extraction system in a volume ratio of 2:9, and circulated heating and mixing are performed for 2.3 hours; after cooling to 15 degrees, centrifugation is performed, and the supernatant is taken for concentration; after concentration, it is re-added into the circulating extraction system together with ethanol, and circulated mixing is performed. Finally, after being taken out and allowed to stand, the precipitate is taken out and vacuum dried to obtain bletilla striata polysaccharide.

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