A composition for preventing and treating epilepsy, a preparation method thereof, and an application thereof

Abstract

The application discloses a composition for preventing and treating epilepsy and a preparation method thereof. The composition is prepared from raw materials in a mass ratio of Sonchus arvensis L., Astragalus membranaceus (Fisch.) Bunge, Radix Paeoniae Rubra and Ziziphus ju Jubiana Pall. in the following proportion: (3-5):(1-4):(1-2):(1-3). The composition is composed of Sonchus arvensis L., Astragalus membranaceus (Fisch.) Bunge, Radix Paeoniae Rubra and Ziziphus ju Jubiana Pall., and can be used as both medicine and food without side effects. The composition can reduce the number of convulsions, shorten the latent period and duration by regulating the content of 5-HT and GABA in the brain of an epilepsy patient.

Description

Technical Field

[0001] This invention relates to the field of epilepsy prevention and treatment technology, and more specifically to a composition for preventing and treating epilepsy, its preparation method, and its application. Background Technology

[0002] Epilepsy is a brain disease caused by abnormal and excessive discharge of neurons in the brain, which in turn causes transient, paroxysmal, and recurrent functional abnormalities in the central nervous system, leading to disturbances in the patient's mental state, consciousness, and senses. It is characterized by recurrent seizures, high mortality, and high disability rates.

[0003] Currently, medication remains the primary treatment for epilepsy. Commonly used antiepileptic drugs in China include carbamazepine, clonazepam, phenobarbital, phenytoin sodium, sodium valproate, lamotrigine, levetiracetam, oxcarbazepine, and topiramate. Approximately two-thirds of patients can control their condition with these antiepileptic drugs, but one-third of epilepsy patients do not respond to existing treatments. Even when medication is effective, it only temporarily controls symptoms and is prone to adverse drug reactions. For patients whose epilepsy cannot be controlled by medication, surgery is often the preferred treatment, but only a small percentage are suitable candidates. Therefore, finding safe and effective antiepileptic drugs is currently one of the hot topics in new drug research both domestically and internationally. Summary of the Invention

[0004] In view of this, the present invention provides a composition for treating epilepsy, providing a natural food or medicine without side effects for the prevention and treatment of epilepsy.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A composition for preventing and treating epilepsy is made from the following raw materials in the following mass ratio: sow thistle: astragalus: red peony: jujube = (3-5): (1-4): (1-2): (1-3).

[0007] Preferably, the above composition is made from the following raw materials in the following mass ratio: sow thistle: astragalus: red peony: jujube = 5:2:1:1.

[0008] The present invention also provides a method for preparing the above-mentioned composition for preventing and treating epilepsy, comprising the following steps:

[0009] (1) The chicory, astragalus, red peony and jujube are mixed in proportion and then pulverized to obtain compound powder;

[0010] (2) Add the compound powder to water, extract under reduced pressure, and filter to obtain the compound extract;

[0011] (3) The compound extract is concentrated under reduced pressure, and the concentrate is spray-dried to obtain the product composition.

[0012] Preferably, in step (2), the compound powder is added to water, and the material-to-liquid ratio is controlled to be 1:8-10 g·mL. -1 The pH value is 5-6, and the extraction time is 1-2 hours at 60℃.

[0013] Preferably, the concentration density under reduced pressure in step (3) is 1.01-1.14.

[0014] The epilepsy prevention and treatment composition provided by the present invention can be used in the preparation of drugs for the prevention and treatment of epilepsy.

[0015] As can be seen from the above technical solution, compared with the prior art, the present invention provides a composition composed of sow thistle, astragalus, red peony root, and jujube, which can be used as both medicine and food, and has no side effects. This composition reduces the frequency of seizures, shortens the latency period, and reduces the duration by regulating the levels of 5-HT and GABA in the brains of epilepsy patients. Detailed Implementation

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] 1 Experimental Methods

[0018] 1.1 Preparation of three different proportions of compositions for the prevention and treatment of epilepsy: Sow thistle, astragalus, red peony root, and jujube were compounded and pulverized according to mass ratios of 5:2:1:1, 7:4:2:3, and 3:1:1:2, respectively, and then set aside. The three compound powders were then added to water separately, with a material-to-liquid ratio of 1:8 (g·mL⁻¹). -1 The extract was prepared at pH 6, under reduced pressure at 60℃ for 2 hours, and filtered to obtain a compound extract. The extract was then concentrated under reduced pressure to a density of 1.04. The extract was further concentrated and spray-dried to obtain three different proportions of plant extract compositions.

[0019] 1.2 Experimental Animals and Model Establishment: Seventy Kunming mice (both male and female) weighing 18–23 g each were used. An acute epileptic seizure model was induced in mice by intraperitoneal injection of 55 mg / kg of pentylenetetrazol (PTZ). The success of the model was mainly based on behavioral changes during the seizure, graded according to the Racine classification: Grade 0: no response; Grade 1: facial clonic movements, including blinking, whisker twitching, and rhythmic chewing; Grade 2: Grade 1 plus rhythmic head nodding; Grade 3: Grade 2 plus forelimb clonic movements; Grade 4: Grade 3 plus hindlimb standing; Grade 5: Grade 4 plus falling. Mice reaching grades 4–5 were selected; those not reaching grade 4 were culled.

[0020] 1.3 Experimental Grouping and Index Detection

[0021] Sixty mice that successfully developed the epilepsy model were randomly selected and divided into four groups: a saline group (control group), a PTZ model group, a phenobarbital group, and a plant extract combination group, with ten mice in each group. All mice were administered the following medications via gavage: plant extract combination 0.5 g / kg (body weight), phenobarbital 50 mg / kg (body weight), and saline 0.4 mL / time, twice daily for 15 days. On day 16, PTZ 55 mg / kg was administered intraperitoneally. The incidence of seizures, latency period (time from drug administration to the first seizure), and duration were recorded.

[0022] After the experimental mice were tested and observed, they were euthanized by cervical dislocation. The whole brain was then removed by craniotomy in an ice bath. The brain was separated on an ice-cold petri dish, the blood was rinsed with ice-cold saline, and the brain was dried with filter paper. After weighing the brain, 9 times the amount of ice-cold saline was added, and the mixture was homogenized at 4°C. The homogenate was transferred to a 2 mL centrifuge tube and centrifuged (3000 rpm, 15 min, 4°C). The supernatant was collected, and the OD values ​​of 5-HT and GABA in the sample were determined according to the relevant operating steps of the kit. The values ​​were then substituted into the standard curve regression equation to calculate the content of 5-HT and GABA in the sample.

[0023] 2.1 Effects on the latency of seizures in mice

[0024] Compared with the PTZ model group, different proportions of plant extract compositions could reduce the incidence of seizures in mice. Compared with the positive control group, the plant extract composition group (5:2:1:1) was better than the positive control group (phenobarbital). It can be seen that the appropriate proportion of plant extract composition can effectively reduce the incidence of seizures in PTZ-induced epilepsy mice. The results are shown in Table 1.

[0025] Table 1. Effects of plant extract compositions on the incidence of seizures in PTZ-induced epilepsy mice.

[0026]

[0027]

[0028] 2.2 Effects on the latency of seizures in mice

[0029] Compared with the PTZ model group, different proportions of plant extract compositions could reduce the effect of PTZ-induced seizure latency in mice. Compared with the positive control group, the plant extract composition group (5:2:1:1) was better than the positive control group (phenobarbital). It can be seen that the appropriate proportion of plant extract composition can reduce the effect of PTZ-induced seizure latency in mice. The results are shown in Table 2.

[0030] Table 2. Effects of plant extract compositions on the latency of seizures in PTZ-induced epileptic mice.

[0031]

[0032] 2.3 Effect on the duration of seizures in mice

[0033] Compared with the PTZ model group, different proportions of plant extract compositions could shorten the duration of continuous seizures in mice. Compared with the positive control group, the plant extract composition group (5:2:1:1) was superior to the positive control group (phenobarbital). It can be seen that the appropriate proportion of plant extract composition can reduce the duration of seizures in PTZ-induced epilepsy mice. The results are shown in Table 3.

[0034] Table 3. Effects of plant extract compositions on the duration of seizures in PTZ-induced epileptic mice.

[0035]

[0036]

[0037] 2.4 Effects on 5-HT and GABA in the brain of PTZ-induced epilepsy mice

[0038] Compared with the PTZ model group, plant extract compositions with different ratios were able to regulate the levels of 5-HT and GABA in the brains of epileptic mice. Compared with the positive control group, the plant extract composition groups (5:2:1:1) and (7:4:2:3) were superior to the positive control group (phenobarbital). This shows that plant extract compositions with appropriate ratios can be used for the prevention and treatment of epilepsy in animals. The results are shown in Table 4.

[0039] Table 4. Effects of plant extract compositions on 5-HT and GABA levels in the brains of PTZ-induced epilepsy mice.

[0040]

[0041] 2.5 Synergistic effect index of plant extracts

[0042] Table 5 shows the theoretical IC50 concentration of plant extracts. 50mix The values ​​are all higher than the experimental IC. 50add The value is small, and the γ value of each combination is also less than 1, and γ 苦苣菜+黄芪(5：2) <γ 苦苣菜+黄芪+赤芍(5：2：1) <γ 苦苣菜+黄芪+赤芍+酸枣(5：2：1：1) This demonstrates that the combination of extracts from sow thistle, astragalus, red peony root, and jujube in a ratio of 5:2:1:1 exhibits a better synergistic effect. Therefore, this invention determines the optimal combination ratio of the extracts from sow thistle, astragalus, red peony root, and jujube to be 5:2:1:1.

[0043] Table 5 Synergistic effect index of plant extract combinations

[0044]

[0045] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0046] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition for preventing and treating epilepsy, characterized in that, Made from the following raw materials in the following mass ratio: sow thistle: astragalus: red peony: jujube = (3~5): (1~4): (1~2): (1~3).

2. The composition for preventing and treating epilepsy according to claim 1, characterized in that, Made from the following raw materials in the following mass ratio: sow thistle: astragalus: red peony: jujube = 5:2:1:

1.

3. A method for preparing a composition for preventing and treating epilepsy according to claim 1 or 2, characterized in that, Includes the following steps: (1) The chicory, astragalus, red peony and jujube were compounded in proportion and then pulverized to obtain compound powder; (2) Add the compound powder to water, extract under reduced pressure, and filter to obtain the compound extract; (3) The compound extract is concentrated under reduced pressure, and the concentrate is spray-dried to obtain the product extract compound.

4. The method for preparing a composition for preventing and treating epilepsy according to claim 3, characterized in that, Step (2) Add the compound powder to water, controlling the material-to-liquid ratio to be 1:8~10 g·mL. -1 The pH value is 5-6, and the extraction time is 1-2 hours at 60℃.

5. The method for preparing a composition for preventing and treating epilepsy according to claim 3, characterized in that, In step (3), the concentration density under reduced pressure is 1.01-1.

14.

6. The use of the composition for preventing and treating epilepsy as described in claim 1 in the preparation of a medicament for preventing and treating epilepsy.

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