A method for rapidly achieving dual short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source through anaerobic / aerobic alternating starvation

Abstract

A method for rapidly achieving dual-short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source through anaerobic / aerobic alternating starvation, belonging to the field of sewage biological treatment. 1. Close the inlet and outlet water systems, continue stirring and intermittent aeration, and maintain the anaerobic / aerobic starvation state; 2. Starve for 10 to 20 days; 3. Restore sludge activity; 4. Start dual-short-range coupled anaerobic ammonium oxidation. This method inhibits the activity of nitrite oxidizing bacteria and the expression level of the nitrite reductase functional gene through starvation treatment, while ammonia oxidizing bacteria (AOB) respond quickly after the influent is restored, and the expression of the nitrate reductase functional gene is not affected. In the short term, the organic matter in domestic sewage is used as a carbon source to achieve dual short-range, control the residual ammonia nitrogen and sludge age, maintain the stable operation of the dual short-range, and provide a stable substrate source for the subsequent coupled anaerobic ammonium oxidation reaction. The present invention is simple to operate and low in cost, and can quickly achieve dual short-range and maintain a high nitrite accumulation rate for a long time.

Description

Technical field:

[0001] The invention relates to a method for rapidly achieving dual short-range coupled anaerobic ammonia oxidation using domestic sewage as a carbon source through anaerobic / aerobic alternating starvation, and belongs to the field of sewage biological treatment. Background technology:

[0002] The primary cause of eutrophication is the excessive discharge of nutrients such as nitrogen and phosphorus. Furthermore, facing increasingly stringent pollutant emission standards, traditional nitrification and denitrification technologies used in sewage treatment plants consume high energy and fail to meet standards. Therefore, the development of new biological wastewater treatment processes with low energy consumption, high denitrification efficiency, and low sludge production is urgently needed.

[0003] Anaerobic ammonium oxidation (ANAMMOX) is an autotrophic denitrification process that has been extensively studied in recent years. Theoretically, it requires no organic carbon source, no aeration, and produces low sludge, making it a highly efficient and energy-efficient process. However, the source of nitrite, one of the ANAMMOX substrates, limits its application in mainstream urban sewage. Short-cut nitrification and short-cut denitrification are two pathways for the generation of nitrite, which opens up new avenues for the application of ANAMMOX. Short-cut nitrification coupled with ANAMMOX allows ANAMMOX to utilize the generated nitrite to simultaneously remove ammonia nitrogen. However, the nitrate nitrogen generated by the short-cut nitrification ANAMMOX system cannot be further removed, and the startup and maintenance of short-cut nitrification are difficult. While coupling short-cut denitrification with ANAMMOX is relatively easy to achieve, the presence of nitrate nitrogen as a substrate in the short-cut denitrification reaction restricts its further application. Currently, it is primarily used in the treatment of nitrified sludge liquids coupled with ANAMMOX. Based on the advantages and disadvantages of short-range nitrification and short-range denitrification, a process is proposed to realize short-range nitrification, short-range denitrification and anaerobic ammonium oxidation reactions in the same reaction system, namely a one-stage double short-range coupled anaerobic ammonium oxidation process.

[0004] However, integrated reactors are relatively difficult to start up. Short-term nitrification primarily selectively suppresses NOB through the addition of hydroxylamine, free ammonia (FA), free nitrite (FNA), and low dissolved oxygen. Short-term denitrification primarily involves the addition of biodegradable organic matter and hydroxylamine. Both short-term nitrification and denitrification require complex control strategies, increasing operating costs.

[0005] Starvation treatment is a simple and efficient method for achieving both short-cut nitrification and short-cut denitrification. In anaerobic starvation, the reactor is stirred but no water is added, which suppresses the expression level of the functional gene of nitrite reductase, while the expression of the functional gene of nitrate reductase is basically unaffected, thereby widening the difference between the nitrate reduction rate and the nitrite reduction rate, thereby achieving short-cut denitrification in a shorter time. In aerobic starvation, the reactor is aerated but no water is added, which suppresses the activity of AOB and NOB. After the reaction cycle is restored with normal water inflow, the recovery rate of AOB is greater than that of NOB. At the same time, controlling the excess ammonia nitrogen can quickly start short-cut nitrification. Based on this, it is proposed to suppress the expression level of denitrifying bacteria functional genes and the activity of nitrifying bacteria through alternating anaerobic / aerobic starvation, so as to quickly achieve short-cut denitrification and short-cut nitrification. After the normal reaction cycle is restored in the later stage, the dual short-cut reaction is maintained by controlling the excess ammonia nitrogen and shortening the sludge age. Summary of the invention:

[0006] To address the startup issues of dual-shortcut coupled anaerobic ammonium oxidation (AAMOX) processes for treating domestic sewage, this patent proposes a method for rapidly achieving dual-shortcuts. This invention utilizes selective inhibition of nitrite reductase gene expression under anaerobic starvation conditions, and inhibition of nitrifying bacteria activity and selective restoration of AOB activity under aerobic starvation conditions, supplemented by controlling residual ammonia nitrogen, dissolved oxygen, and sludge age, to rapidly achieve dual-shortcut startup using domestic sewage as a carbon source.

[0007] A dual short-range method for rapidly achieving domestic sewage as a carbon source through anaerobic / aerobic alternating starvation, the device used includes: an SBR reactor (16) and online pH and DO detection equipment; Figure 1 As shown, the domestic sewage inlet bucket (1) is connected to the SBR water inlet (3) through the water inlet peristaltic pump (2) and enters the SBR reactor (16); the nitrate wastewater (14) enters the reactor through the peristaltic pump (15); a stirrer (7) is set to stir the activated sludge inside the reactor to mix it evenly; a rotor flowmeter (9) is connected to the air pump (8) and the aeration plate (10) for aeration to control the dissolved oxygen in the reactor; water is discharged through the outlet valve (11); sludge is discharged through the sludge outlet (13); the pH probe (5) and the DO probe (6) are connected to the WTW host (4) through a data line to reflect the sludge properties inside the reactor.

[0008] A method for rapidly achieving dual short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source by anaerobic / aerobic alternating starvation, characterized by comprising the following steps:

[0009] (1) Before starvation treatment, inoculate the wastewater treatment plant with excess sludge, control the sludge concentration to 2800-3000 mg / L, and debug the reactor to ensure the normal operation of all equipment;

[0010] (2) Anaerobic / aerobic starvation treatment: close the water inlet and outlet systems of the reactor, do not add any substrate, continuously stir, aerate intermittently, control the aeration time, make the anaerobic / anoxic starvation time 4-5h and 3-4h respectively, control the dissolved oxygen of aerobic starvation at 4.0-6.0mg / L, and do not discharge sludge during the starvation period. Take samples regularly to measure the sludge status and material changes inside the reactor. The starvation time is determined according to the properties of the sludge. Sludge can be taken regularly for batch experiments: add 500 ml of the sludge in the starvation treatment to a 500 ml small test bottle, add 18-20 mg / L ammonia nitrogen and 8-10 mg / L nitrite nitrogen, maintain aeration at 5-6 mg / L, and conduct the batch test for 2 hours. Take samples every 10 minutes to measure the concentrations of ammonia nitrogen and nitrate nitrogen. The ammonia nitrogen consumption rate and nitrate nitrogen production rate represent the activity of ammonia oxidizing bacteria and nitrite oxidizing bacteria, respectively; add 500 ml of the sludge in the starvation treatment to a 500 ml small test bottle, add 18-20 mg / L nitrate nitrogen and 58-60 mg / L COD, COD is provided by sodium acetate, and the experiment is conducted for 3 hours. Take samples every 15 minutes to measure the concentrations of nitrate nitrogen and nitrite nitrogen to obtain the nitrate conversion rate. When the batch experiment showed that the activities of AOB and NOB were inhibited, the ammonia nitrogen degradation rate and nitrate nitrogen production rate dropped to 0, and the nitrate nitrogen conversion rate of denitrification reached more than 80%, the starvation treatment was stopped and the activity recovery and stable operation stage was carried out;

[0011] (3) Activity recovery treatment: To quickly restore the activity of nitrifying and denitrifying bacteria, restart the reactor: First, centrifuge the sludge after starvation treatment to remove nitrogen, phosphorus and other substances produced by fermentation during the starvation process; open the water inlet system and drainage system, and run the SBR system according to the pre-set program, running four cycles a day, each cycle is 6 hours, including water inlet 10 minutes, anoxic stirring 50 minutes, aerobic aeration 180 minutes, sedimentation 30 minutes, drainage 10 minutes, idle 80 minutes, and drainage ratio 70%; in the activity recovery stage, maintain the sludge age of 15 days; adjust the water inlet life The ratio of sewage to nitrate wastewater is such that the nitrate concentration after mixing is 18-20 mg / L; the dissolved oxygen in the aerobic stage is maintained at 1-2 mg / L; the influent is changed to clean water every 8 cycles to avoid excessive accumulation of nitrite nitrogen in the reactor, which inhibits the activity of the bacterial community and affects the recovery of activity; regular sampling is taken to measure the concentration of pollutants at the inlet and outlet water and the anoxic end, and the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage are calculated to reflect the short-cut denitrification and short-cut nitrification effects of the reaction system. When the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage are both ≥80% and maintained for more than 10 days, it can be considered that the reactor has recovered its activity and entered the stable operation stage;

[0012] (4) Startup of the one-stage double short-range coupled anaerobic ammonium oxidation reactor. To successfully start the double short-range coupled anaerobic ammonium oxidation reaction, anaerobic ammonium oxidation biofilm filler was added to the double short-range reactor with a filling ratio of 10%. The SBR system reaction process was adjusted. The startup and stable operation of the reactor were carried out according to this process: two cycles were run every day, each cycle was 12 hours, including water inlet 10 minutes, anaerobic stirring 80 minutes, aerobic aeration 2-3 hours, anoxic stirring 6-7 hours, sedimentation 30 minutes, Drain for 10 minutes and leave idle for 50 minutes. The anoxic time is adjusted according to the aerobic time. The sum of the two is 9 hours, and the drainage ratio is 50%. In order to ensure the carbon source for short-term denitrification in the anoxic section, a segmented water inlet method is adopted: the first water inlet is before anaerobic operation, accounting for 35% of the reactor volume, and the second water inlet is when the anoxic stirring is 3-4 hours, accounting for 15% of the reactor volume; the sludge age is maintained at 15-20 days; the DO in the aerobic section is maintained below 1 mg / L, and the residual ammonia nitrogen at the end of aerobic operation is controlled at 6-8 mg / L; regular sampling is performed The pollutant concentrations of the inlet and outlet water were determined. When the outlet TIN was less than 10 mg / L, the one-stage dual short-range coupled anaerobic ammonium oxidation reactor was considered to have been successfully started. The reactor that had been successfully started continued to operate with staged water inflow. The first water inflow was before anaerobic operation and accounted for 35% of the reactor volume. The second water inflow was after 3-4 hours of anoxic stirring and accounted for 15% of the reactor volume. The reactor was operated for two cycles per day, each cycle lasting 12 hours, including 10 minutes of water inflow, 80 minutes of anaerobic stirring, 2-3 hours of aerobic aeration, and 6 hours of anoxic stirring. ~7h, sedimentation 30min, drainage 10min, idle 50min, among which the anoxic time is adjusted according to the aerobic time, the sum of the two is 9h, and the drainage ratio is 50%; maintain a sludge age of 15 to 20 days; maintain the DO of the aerobic section below 1mg / L, and control the residual ammonia nitrogen at the end of aerobic at 6 to 8mg / L; take samples regularly to measure the concentration of inlet and outlet pollutants, run the reactor for 30 to 40d, and keep the effluent TIN less than 10mg / L. It is considered that the one-stage double short-range coupled anaerobic ammonia oxidation reactor is stable.

[0013] The technical principle of the present invention is that under anaerobic starvation conditions, the functional gene expression levels of nitrite reductase and nitrate reductase are inhibited to different degrees, thereby widening the difference between the nitrate reduction rate and the nitrite reduction rate, the nitrite reduction rate is low, and a higher nitrite accumulation is achieved; while under aerobic starvation conditions, both ammonia oxidizing bacteria and nitrite oxidizing bacteria are inhibited, but ammonia oxidizing bacteria have strong environmental adaptability due to their special cell structure and can quickly respond to changes in the external environment. In the recovery process after the end of starvation, ammonia oxidizing bacteria show a faster recovery rate than nitrite oxidizing bacteria; at the same time, the ammonia nitrogen surplus, dissolved oxygen and sludge age in the aerobic section are controlled, and the dual short-range with domestic sewage as the carbon source is quickly achieved.

[0014] The present invention provides a method for rapidly achieving dual short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source through anaerobic / aerobic starvation, which has the following advantages:

[0015] The dual short-term startup time is short, and the anaerobic / aerobic starvation and recovery process can expand the differences in denitrification functional genes and nitrifying bacteria activity. The system can achieve higher nitrate conversion rate and nitrite accumulation rate in a shorter time.

[0016] The starvation treatment method is simple and does not require complicated facilities and operations. It only requires continuous stirring during the starvation process and providing aeration for a certain period of time during aerobic starvation to maintain an aerobic starvation environment.

[0017] Small economic investment, low cost, no need to modify the original device and equipment during the anaerobic / aerobic starvation process, no need to add drugs and carbon sources, and can directly achieve dual short-range carbon source using domestic sewage;

[0018] The dual short-range coupled anaerobic ammonium oxidation system was successfully started without the need for an external carbon source. The system used a staged water inlet method, saving carbon sources.

[0019] The present invention has strong practicability and can quickly realize the dual short-range in the SBR reactor in the sewage treatment plant through anaerobic / aerobic starvation for a certain period of time. Description of the drawings:

[0020] Figure 1 It is a structural diagram of a dual short-range coupled anaerobic ammonium oxidation system that quickly realizes domestic sewage as a carbon source through anaerobic / aerobic starvation treatment.

[0021] Figure 2 It is the operating cycle diagram of the reactor.

[0022] In the figure: 1 is the water inlet bucket, 2 is the water inlet peristaltic pump, 3 is the water inlet, 4 is the WTW main unit, 5 is the pH probe, 6 is the DO probe, 7 is the agitator, 8 is the air pump, 9 is the rotor flowmeter, 10 is the aeration plate, 11 is the water outlet valve, 12 is the water outlet bucket, 13 is the mud outlet, 14 is nitrate wastewater, 15 is the peristaltic pump, and 16 is the SBR reactor. Specific implementation method:

[0023] The present invention is further described in detail below with reference to the accompanying drawings and technical solutions, but the present invention is not limited to the following embodiments.

[0024] refer to Figure 1A schematic diagram of a method and device for rapidly achieving dual short-range carbon source using domestic sewage through anaerobic / aerobic starvation is shown, wherein a domestic sewage inlet bucket (1) is connected to an SBR water inlet (3) through an inlet peristaltic pump (2) and enters an SBR reactor (16); nitrate wastewater (14) enters the reactor through a peristaltic pump (15); an agitator (7) is provided for stirring to mix the activated sludge inside the reactor; a rotor flowmeter (9) is connected to an air pump (8) and an aeration disk (10) for aeration to control the dissolved oxygen in the reactor; water is discharged through a water outlet valve (11); and sludge is discharged through a sludge outlet (13); a pH probe (5) and a DO probe (6) are connected to a WTW host (4) through a data line to reflect the sludge properties inside the reactor.

[0025] refer to Figure 1 Schematic diagram of a dual short-range method device for rapidly achieving domestic sewage as a carbon source through anaerobic / aerobic starvation. Figure 2 The operating cycle diagram of each stage realizes dual short-range coupled anaerobic ammonium oxidation according to the following steps:

[0026] (1) Before starvation treatment, inoculate the wastewater treatment plant with excess sludge, control the sludge concentration to 2800-3000 mg / L, and debug the reactor to ensure the normal operation of all equipment;

[0027] (2) Anaerobic / aerobic starvation treatment: close the water inlet and outlet systems of the reactor, do not add any substrate, continuously stir, aerate intermittently, control the aeration time, make the anaerobic / anoxic starvation time 4-5h and 3-4h respectively, control the dissolved oxygen of aerobic starvation at 4.0-6.0mg / L, and do not discharge sludge during the starvation period. Take samples regularly to measure the sludge status and material changes inside the reactor. The starvation time is determined according to the properties of the sludge. Sludge can be taken regularly for batch experiments: add 500 ml of the sludge in the starvation treatment to a 500 ml small test bottle, add 18-20 mg / L ammonia nitrogen and 8-10 mg / L nitrite nitrogen, maintain aeration at 5-6 mg / L, and conduct the batch test for 2 hours. Take samples every 10 minutes to measure the concentrations of ammonia nitrogen and nitrate nitrogen. The ammonia nitrogen consumption rate and nitrate nitrogen production rate represent the activity of ammonia oxidizing bacteria and nitrite oxidizing bacteria, respectively; add 500 ml of the sludge in the starvation treatment to a 500 ml small test bottle, add 18-20 mg / L nitrate nitrogen and 58-60 mg / L COD, COD is provided by sodium acetate, and the experiment is conducted for 3 hours. Take samples every 15 minutes to measure the concentrations of nitrate nitrogen and nitrite nitrogen to obtain the nitrate conversion rate. When the batch experiment showed that the activities of AOB and NOB were inhibited, the ammonia nitrogen degradation rate and nitrate nitrogen production rate dropped to 0, and the nitrate nitrogen conversion rate of denitrification reached more than 80%, the starvation treatment was stopped and the activity recovery and stable operation stage was carried out;

[0028] (3) Activity recovery treatment: To quickly restore the activity of nitrifying and denitrifying bacteria, restart the reactor: First, centrifuge the sludge after starvation treatment to remove nitrogen, phosphorus and other substances produced by fermentation during the starvation process; open the water inlet system and drainage system, and run the SBR system according to the pre-set program, running four cycles a day, each cycle is 6 hours, including water inlet 10 minutes, anoxic stirring 50 minutes, aerobic aeration 180 minutes, sedimentation 30 minutes, drainage 10 minutes, idle 80 minutes, and drainage ratio 70%; in the activity recovery stage, maintain the sludge age of 15 days; adjust the water inlet life The ratio of sewage to nitrate wastewater is such that the nitrate concentration after mixing is 18-20 mg / L; the dissolved oxygen in the aerobic stage is maintained at 1-2 mg / L; the influent is changed to clean water every 8 cycles to avoid excessive accumulation of nitrite nitrogen in the reactor, which inhibits the activity of the bacterial community and affects the recovery of activity; regular sampling is taken to measure the concentration of pollutants at the inlet and outlet water and the anoxic end, and the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage are calculated to reflect the short-cut denitrification and short-cut nitrification effects of the reaction system. When the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage are both ≥80% and maintained for more than 10 days, it can be considered that the reactor has recovered its activity and entered the stable operation stage;

[0029] (4) Startup of the one-stage double short-range coupled anaerobic ammonium oxidation reactor. To successfully start the double short-range coupled anaerobic ammonium oxidation reaction, anaerobic ammonium oxidation biofilm filler was added to the double short-range reactor with a filling ratio of 10%. The SBR system reaction process was adjusted. The startup and stable operation of the reactor were carried out according to this process: two cycles were run every day, each cycle was 12 hours, including water inlet 10 minutes, anaerobic stirring 80 minutes, aerobic aeration 2-3 hours, anoxic stirring 6-7 hours, sedimentation 30 minutes, Drain for 10 minutes and leave idle for 50 minutes. The anoxic time is adjusted according to the aerobic time. The sum of the two is 9 hours, and the drainage ratio is 50%. In order to ensure the carbon source for short-term denitrification in the anoxic section, a segmented water inlet method is adopted: the first water inlet is before anaerobic operation, accounting for 35% of the reactor volume, and the second water inlet is when the anoxic stirring is 3-4 hours, accounting for 15% of the reactor volume; the sludge age is maintained at 15-20 days; the DO in the aerobic section is maintained below 1 mg / L, and the residual ammonia nitrogen at the end of aerobic operation is controlled at 6-8 mg / L; regular sampling is performed The pollutant concentrations of the inlet and outlet water were determined. When the outlet TIN was less than 10 mg / L, the one-stage dual short-range coupled anaerobic ammonium oxidation reactor was considered to have been successfully started. The reactor that had been successfully started continued to operate with staged water inflow. The first water inflow was before anaerobic operation and accounted for 35% of the reactor volume. The second water inflow was after 3-4 hours of anoxic stirring and accounted for 15% of the reactor volume. The reactor was operated for two cycles per day, each cycle lasting 12 hours, including 10 minutes of water inflow, 80 minutes of anaerobic stirring, 2-3 hours of aerobic aeration, and 6 hours of anoxic stirring. ~7h, sedimentation 30min, drainage 10min, idle 50min, among which the anoxic time is adjusted according to the aerobic time, the sum of the two is 9h, and the drainage ratio is 50%; maintain a sludge age of 15 to 20 days; maintain the DO of the aerobic section below 1mg / L, and control the residual ammonia nitrogen at the end of aerobic at 6 to 8mg / L; take samples regularly to measure the concentration of inlet and outlet pollutants, run the reactor for 30 to 40d, and keep the effluent TIN less than 10mg / L. It is considered that the one-stage double short-range coupled anaerobic ammonia oxidation reactor is stable.

[0030] The above is a detailed introduction to the anaerobic / anoxic rapid start-up dual short-range coupled anaerobic ammonia oxidation biological denitrification device and method provided by the present invention. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above implementation cases is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and applications. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A method for rapidly achieving dual short-range coupled anaerobic ammonium oxidation using domestic sewage as a carbon source through anaerobic / aerobic alternating starvation, characterized in that: The device used includes: an SBR reactor (16), a pH probe (5) and a DO probe (6); the domestic sewage inlet bucket (1) is connected to the SBR water inlet (3) through the water inlet peristaltic pump (2) and enters the SBR reactor (16); the nitrate wastewater (14) enters the SBR reactor (16) through the peristaltic pump (15); a stirrer (7) is set to stir the activated sludge to mix it evenly; a rotor flow meter (9) is connected to the air pump (8) and the aeration plate (10) for aeration to control the dissolved oxygen in the reactor; water is discharged through the outlet valve (11); sludge is discharged through the sludge outlet (13); the pH probe (5) and the DO probe (6) are connected to the WTW host (4) through a data cable; It is characterized in that it includes the following steps: (1) Before starvation treatment, inoculate the wastewater treatment plant with excess sludge, control the sludge concentration to 2800-3000 mg / L, and debug the reactor to ensure the normal operation of all equipment; (2) Anaerobic / aerobic starvation treatment: close the water inlet and drainage systems of the reactor, do not add any substrate, continuously stir, aerate intermittently, control the aerobic time, make the anaerobic / aerobic starvation time 4~5h and 3~4h respectively, control the dissolved oxygen of aerobic starvation at 4.0~6.0mg / L, and do not discharge sludge during the starvation period; regularly sample and measure the sludge state and material changes inside the reactor, and regularly take sludge for batch experiments: add 500mL of sludge in the starvation treatment to a 500mL small test bottle, add 18~20mg / L ammonia nitrogen and 8~10mg / L nitrite nitrogen, maintain aeration at 5~6mg / L, and carry out the batch experiment for 2h. Samples are taken every 10 minutes to measure the ammonia nitrogen and Nitrate nitrogen concentration, with ammonia nitrogen consumption rate and nitrate nitrogen production rate representing the activity of ammonia oxidizing bacteria and nitrite oxidizing bacteria, respectively; 500 mL of sludge from the starvation treatment was added to a 500 mL test bottle, and 18-20 mg / L nitrate nitrogen and 58-60 mg / L COD were added. COD was provided by sodium acetate. The experiment was carried out for 3 hours, and samples were taken every 15 minutes to measure the nitrate nitrogen and nitrite nitrogen concentrations to obtain the nitrate conversion rate. When the batch experiment showed that the activities of AOB and NOB were both inhibited, the ammonia nitrogen consumption rate and nitrate nitrogen production rate dropped to 0, and the nitrate conversion rate of denitrification reached more than 80%, the starvation treatment was stopped and the activity recovery and stable operation stage was carried out; (3) Activity recovery treatment: To quickly restore the activity of nitrifying and denitrifying bacteria, restart the reactor: First, centrifuge the sludge after starvation treatment to remove nitrogen and phosphorus substances produced by fermentation during the starvation process; open the water inlet and drainage systems, and operate the SBR reactor according to the pre-set program, running four cycles a day, each cycle for 6 hours, including water inlet for 10 minutes, anoxic stirring for 50 minutes, aerobic aeration for 180 minutes, sedimentation for 30 minutes, drainage for 10 minutes, and idleness for 80 minutes, with a drainage ratio of 70%; during the activity recovery stage, maintain a sludge age of 15 days; The ratio of influent domestic sewage to nitrate wastewater was adjusted so that the nitrate concentration after mixing was 18-20 mg / L; the dissolved oxygen in the aerobic stage was maintained at 1-2 mg / L; the influent was changed to clean water every 8 cycles; the influent and effluent and anoxic terminal pollutant concentrations were regularly sampled and measured, and the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage were calculated. The nitrate conversion rate and nitrite accumulation rate were used to represent the short-cut denitrification and short-cut nitrification effects of the reactor, respectively. When the nitrate conversion rate in the anoxic stage and the nitrite accumulation rate in the aerobic stage were both ≥80% and maintained for more than 10 days, the reactor was considered to have recovered its activity and entered the stable operation stage; (4) Startup and operation of one-stage dual short-range coupled anaerobic ammonium oxidation reaction. To successfully start the dual short-range coupled anaerobic ammonium oxidation reaction, anaerobic ammonium oxidation biofilm filler was added to the SBR reactor with a filling ratio of 10%. The reaction process of the SBR reactor was adjusted. The startup and stable operation of the reactor were operated according to this process: two cycles were run every day, each cycle was 12 hours, including water inlet for 10 minutes, anaerobic stirring for 80 minutes, aerobic aeration for 2-3 hours, anoxic stirring for 6-7 hours, sedimentation for 30 minutes, drainage for 10 minutes, and idle for 50 minutes. Among them, the anoxic time was adjusted according to the aerobic time, and the sum of the two was 9 hours. The drainage ratio was 50%. In order to ensure the carbon source for short-range denitrification in the anoxic section, a segmented water inlet method was adopted: the first water inlet was before anaerobic, accounting for 35% of the reactor volume, and the second water inlet was when the anoxic stirring was 3-4 hours, accounting for 15% of the reactor volume; the sludge age was maintained at 15-20 days; the DO of the aerobic section was maintained at 1 mg / L or less, and the residual ammonia nitrogen at the end of aerobic state is controlled at 6~8mg / L; sampling is taken regularly to determine the concentration of pollutants in the inlet and outlet water. When the effluent TIN is less than 10mg / L, it is considered that the one-stage double short-range coupled anaerobic ammonia oxidation reaction has been successfully started; the successfully started reactor continues to operate, using segmented water inlet, the first water inlet before anaerobic operation, accounting for 35% of the reactor volume, and the second water inlet when the anoxic stirring is 3~4h, accounting for 15% of the reactor volume; two cycles are run every day, each cycle is 12 hours, including water inlet 10min, anaerobic stirring 80min, aerobic aeration 2~3h, anoxic stirring 6~7h, sedimentation 30min, drainage 10min, and idle 50min, among which the anoxic time is adjusted according to the aerobic time, the sum of the two is 9h, and the drainage ratio is 50%; the sludge age is maintained at 15~20 days; the DO of the aerobic section is maintained at 1 mg / L, and the residual ammonia nitrogen at the end of aerobic oxidation was controlled at 6~8 mg / L; regular sampling was taken to determine the concentration of pollutants in the inlet and outlet water. The reactor was run for 30~40 days, and the effluent TIN was kept less than 10 mg / L. It was considered that the one-stage double short-range coupled anaerobic ammonia oxidation reaction was stable.

2. The method according to claim 1, characterized in that The aerobic time is maintained in steps (3) and (4) to control the residual ammonia nitrogen concentration.

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