A control system and method for sterilizing a plant tissue culture apparatus

Abstract

The application belongs to the technical field of plant tissue culture device, and discloses a control system and method for sterilizing a plant tissue culture device, which comprises a steam sterilization control module, a plant tissue culture device, a detection device and a steam delivery pipeline; the steam delivery pipeline delivers steam to a device to be sterilized of the plant tissue culture device; the detection device detects the solution condition in the plant tissue culture device; and the steam sterilization control module controls the sterilization of the device to be sterilized by steam according to the detection result. The steam sterilization control module is used to accurately control the on-off of the steam sterilization of the plant tissue culture device; the detection device sends an electric signal to the steam sterilization control module to indicate the specific valve body that needs to be supplemented with solution; and the steam sterilization control module automatically performs steam sterilization on the specific valve body after receiving the electric signal, thereby more accurately and fully automatically controlling the steam sterilization process.

Description

Technical Field

[0001] This invention belongs to the technical field of plant tissue culture devices, specifically, it relates to a control system and method for disinfecting plant tissue culture devices. Background Technology

[0002] With the continuous improvement of living standards, people are paying increasing attention to strengthening their constitution and maintaining health through the consumption of medicinal herbs with nourishing effects. Due to the huge population, the demand for medicinal plants has risen sharply. Furthermore, cultivating medicinal plants using traditional methods requires not only large amounts of land and a long growth cycle, but also a suitable climate. Any unsuitable conditions will limit and reduce the scale of medicinal plant cultivation and the yield of medicinal plants.

[0003] Therefore, researchers have developed a method and cultivation device for large-scale cultivation using isolated plant tissues or cells. By separating plant organs such as roots, stems, and leaves, and then placing them in a nutrient-rich culture medium while providing suitable temperature, light, and other environmental conditions, callus, adventitious buds, and adventitious roots are induced. Finally, these callus, adventitious buds, and adventitious roots are used as seeds for further cultivation in the device. This method overcomes the dependence on soil and climate conditions for medicinal plant cultivation and allows for large-scale cultivation using numerous devices, increasing the yield of medicinal plants.

[0004] In existing technologies, plant tissue culture requires strict aseptic conditions to be effective, thus necessitating the sterilization of the culture apparatus. Steam sterilization is a common method, and automated production workshops typically utilize electrical control technology to operate various valves. However, traditional steam sterilization methods are not precise, as they involve steam sterilizing valves at fixed intervals, wasting energy and failing to achieve accurate sterilization.

[0005] In view of this, the present invention is hereby proposed. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a control system and method for disinfecting plant tissue culture devices. The steam disinfection control module in the electrical control cabinet precisely controls the steam disinfection switch of the plant tissue culture device. When the detection device on the tank detects that the tank needs to be replenished with solution, the detection device sends an electrical signal to the steam disinfection control module to indicate the specific valve that needs to be replenished with solution. After receiving the electrical signal, the steam disinfection control module automatically performs steam disinfection on the specific valve, thus controlling the steam disinfection process more precisely and automatically.

[0007] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0008] A control system for sterilizing a plant tissue culture apparatus includes: a steam sterilization control module, a plant tissue culture apparatus, a detection device, and a steam delivery pipeline; the steam delivery pipeline delivers steam to the apparatus to be sterilized within the plant tissue culture apparatus, the detection device detects the solution status within the plant tissue culture apparatus, and the steam sterilization control module controls the steam to sterilize the apparatus based on the detection results.

[0009] Furthermore, the plant tissue culture device includes a culture tank and a seed tank, and at least one transplanting tube for conveying a high concentration of plant tissue from the seed tank to the culture tank and at least one return tube for conveying diluted plant tissue from the culture tank to the seed tank are provided between the culture tank and the seed tank.

[0010] The steam delivery pipeline includes several steam branch pipes, which are respectively connected to the culture tank, seed tank, transplanting pipe and return pipe. The steam branch pipes are also respectively connected to the devices to be sterilized on the culture tank, seed tank, transplanting pipe and return pipe. Each steam branch pipe is equipped with a steam control valve, which is used to receive control commands from the steam sterilization control module and execute the actions of the control commands.

[0011] Furthermore, the steam sterilization control module includes:

[0012] Disinfection control module for transplanting tube, disinfection control module for return tube, disinfection control module for tank pneumatic valve, and disinfection control module for inside tank;

[0013] The steam sterilization control module is a part of the electrical control cabinet. The plant tissue culture device is equipped with multiple control valves. The electrical control cabinet is connected to the control valves of the plant tissue culture device through a line. The line is used to transmit electrical signals for different control commands. The control valve includes at least a steam control valve installed on the steam branch pipe and a pneumatic valve for replenishing culture medium into the tank. The steam sterilization control module uses electrical signals to control the steam switches on the steam control valve and the pneumatic valve for replenishing culture medium into the tank to perform opening and closing operations.

[0014] Furthermore, the in-tank disinfection control module is used to control steam to disinfect the inside of the seed tank and culture tank. A gas distributor is installed inside the culture tank and seed tank, and an air inlet branch pipe is connected to the gas distributor. Each air inlet branch pipe has the same extension length, and the other end of each air inlet branch pipe is connected to a three-way pipe 23. The air inlet branch pipe is connected to an air inlet pipe through the three-way pipe 23. The air inlet pipe is located near the center of the bottom of the culture tank. Two air inlet branch pipes extend a certain distance to both sides of the tank and bend inward to connect to the gas distributor. The two air inlet branch pipes are symmetrically arranged on the bottom wall. A steam branch pipe is connected to the air inlet pipe, and a steam control valve is installed on the steam branch pipe. The in-tank disinfection control module sends an electrical signal to the steam control valve to open the steam control valve, so that steam is introduced into the tank through the gas distributor to disinfect the inside of the tank.

[0015] Furthermore, the seed transfer tube disinfection control module is used to control steam to disinfect the inside of the seed transfer tube that transfers seeds from the seed tank to the culture tank. The seed transfer tube is connected to a steam branch pipe, and a steam control valve is installed on the steam branch pipe. The seed transfer tube is also equipped with a seed transfer valve, and another steam branch pipe is connected to the seed transfer valve. A steam control valve is also installed on the steam branch pipe of the seed transfer valve.

[0016] When transplanting is required, the transplanting tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube, and also sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting valve to disinfect the transplanting tube and the transplanting valve. After disinfection is completed, the transplanting tube disinfection control module sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube and the steam control valve on the steam branch pipe connected to the transplanting valve, and then performs the transplanting operation.

[0017] Furthermore, the return seed tube disinfection control module is used to control steam to disinfect the inside of the return seed tube used for returning seeds from the culture tank to the seed tank. The return seed tube is also connected to a steam branch pipe, and a steam pneumatic valve is installed on the steam branch pipe. The return seed tube is also equipped with a return seed valve, and a steam branch pipe is also connected to the return seed valve. A steam control valve is also installed on the steam branch pipe of the return seed valve.

[0018] After transplanting, the disinfection control module of the return seed tube sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube, and also sends an opening electrical signal to the steam control valve on the steam branch pipe of the return seed valve to disinfect the return seed tube and the return seed valve. After disinfection is completed, the disinfection control module of the return seed tube sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube and the steam control valve on the steam branch pipe connected to the return seed valve, and then performs the return seeding operation.

[0019] Furthermore, the culture tank and seed tank are equipped with at least one of four pneumatic valves: a feeding pneumatic valve, an acid feeding pneumatic valve, an alkali feeding pneumatic valve, and an antifoaming liquid feeding pneumatic valve. The tank body pneumatic valve disinfection control module is used to control steam to disinfect the pneumatic valves. The tank body is also equipped with a detection device for detecting at least one of the pH value, solution concentration, and foam density inside the tank. The detection device is connected to the electrical control cabinet via a line.

[0020] When the detection device detects an imbalance in the solution, it sends an electrical signal to the electrical control cabinet. Upon receiving the signal, the electrical control cabinet sends an electrical signal to the tank pneumatic valve control module to indicate the required replenishment of liquid. The tank pneumatic valve control module then sends an electrical signal to the pneumatic valve containing the liquid to be replenished, controlling the opening of the steam switch on the pneumatic valve to introduce steam for sterilization. After sterilization is complete, the tank pneumatic valve control module sends an electrical signal to the pneumatic valve to close the steam switch, and then the liquid replenishment operation is performed.

[0021] A method for controlling the disinfection of a plant tissue culture apparatus, employing the aforementioned control system for disinfecting the plant tissue culture apparatus, includes the following steps:

[0022] S1. Add culture medium into the seed tank. The disinfection control module in the tank sends an opening electrical signal to the steam control valve connected to the gas distributor in the seed tank to disinfect the culture medium in the seed tank. After disinfection, the disinfection control module in the tank sends an electrical signal to the steam control valve to close the steam control valve and adds plant tissue culture to the seed tank for a certain period of time.

[0023] S2. After the plant tissue has been cultured in the seed tank for a period of time, culture medium is added to the culture tank. The disinfection control module in the tank sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the gas distributor in the culture tank to perform steam disinfection of the culture medium in the culture tank.

[0024] S3. After the culture medium in the culture tank is disinfected, the transplanting tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube to disinfect the inside of the transplanting tube. At the same time, the transplanting tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting valve to disinfect the transplanting valve. After disinfection, the plant tissue culture medium in the seed tank is transferred to the culture tank for cultivation.

[0025] Furthermore, after step S3, step S4 is also included: the disinfection control module of the return tube sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return tube to disinfect the inside of the return tube; the disinfection control module of the return tube then sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return valve to disinfect the return valve; after disinfection, the diluted part of the plant tissue culture solution in the culture tank is returned to the seed tank for continued culture.

[0026] Furthermore, in steps S1 and S2, after adding culture medium to the seed tank and culture tank, the culture medium in the tank is sterilized by introducing steam into the tank through a gas distributor to heat the culture medium in the tank to a specified temperature and time, thereby completing the sterilization of the culture medium.

[0027] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0028] 1. The steam sterilization control module can more accurately control the steam sterilization switch on the electric valve, and accurately sterilize the device that needs to be sterilized.

[0029] 2. The detection device installed on the tank can detect the solution inside the tank. When the solution inside the tank is out of balance, it will send an electrical signal to the electrical control cabinet. The steam sterilization control module in the electrical control cabinet will send an electrical signal to the pneumatic valve on the tank that needs to be replenished with liquid to sterilize the pneumatic valve. The system can automatically and accurately sterilize the plant tissue culture device.

[0030] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0031] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0032] Figure 1 This is a schematic diagram of the high-temperature steam sterilization cycle of the cultivation device of the present invention;

[0033] Figure 2 yes Figure 1 Enlarged schematic diagrams of the structures at points A and B in the middle;

[0034] Figure 3 This is a schematic diagram of the structure at the bottom of the culture tank connected to the five-way valve of the present invention;

[0035] Figure 4This is a schematic diagram of the structure of the gas distributor installed on the plant tissue culture vessel in this invention;

[0036] Figure 5 This is a flowchart of the in-tank disinfection control module of the present invention;

[0037] Figure 6 This is a flowchart of the seed tube disinfection control module of the present invention;

[0038] Figure 7 This is a flowchart of the disinfection control module for the seed return tube of the present invention;

[0039] Figure 8 This is a flowchart of the tank pneumatic valve disinfection control module of the present invention;

[0040] Figure 9 This is a flowchart of the seed tank sterilization process of the present invention;

[0041] Figure 10 This is a flowchart of the sterilization process for the culture tank of this invention;

[0042] Figure 11 This is a flowchart of the seed transfer steps of the present invention;

[0043] Figure 12 This is a flowchart of the seeding steps of the present invention;

[0044] Figure 13 This is a schematic diagram showing the distribution of the gas distributor and shearing device at the bottom of the seed tank according to the present invention;

[0045] Figure 14 This is a schematic diagram showing the distribution of the gas distributor and shearing device at the bottom of the culture tank according to the present invention;

[0046] Figure 15 This is a schematic diagram of the gas distributor and air inlet pipe at the bottom of the tank of the present invention;

[0047] Figure 16 This is a side view showing the distribution of the gas distributor and shearing device on the bottom wall of the bioreactor in this invention.

[0048] The following are the labels in the diagram: 3. High-temperature steam filtration device; 4. High-temperature steam main pipe; 5. Waste steam main exhaust pipe; 6. Seed transfer pipe; 7. Seed return pipe; 8. Seed tank; 9. Culture tank; 10. Waste steam recovery device; 11. Waste steam pressure reducing and cooling device; 12. Waste liquid recovery tank; 13. Feeding pneumatic valve; 14. Acid replenishing pneumatic valve; 15. Alkali replenishing pneumatic valve; 16. Defoaming liquid replenishing pneumatic valve; 17. Air filter; 18. Seed tank air inlet pipe; 19. Culture tank air inlet pipe; 20. Gas distributor; 21. Aeration section; 22. Shearing device; 23. T-connector;

[0049] 101. High-temperature steam inlet pipe for air filter; 102. Waste steam outlet pipe for air filter; 103. High-temperature steam inlet pipe for culture tank pneumatic valve; 104. Waste steam outlet pipe for culture tank pneumatic valve; 105. High-temperature steam inlet pipe for seed tank pneumatic valve; 106. Waste steam outlet pipe for seed tank pneumatic valve; 107. High-temperature steam inlet pipe for transplanting tube; 108. High-temperature steam inlet pipe for reseeding tube; 109. Discharge pipe; 110. Sewage pipe; 111. CIP discharge pipe; 112. CIP inlet pipe; 113. High-temperature steam inlet pipe for feeding pneumatic valve; 114. Waste steam outlet pipe for feeding pneumatic valve; 115. High-temperature steam inlet pipe for acid replenishment pneumatic valve; 16. Waste steam outlet pipe for acid replenishment pneumatic valve; 117. High-temperature steam inlet pipe for alkali replenishment pneumatic valve; 118. Waste steam outlet pipe for alkali replenishment pneumatic valve; 119. High-temperature steam inlet pipe for defoaming liquid replenishment pneumatic valve; 120. Waste steam outlet pipe for defoaming liquid replenishment pneumatic valve; 121. High-temperature steam inlet pipe for discharge valve; 122. Waste steam outlet pipe for discharge valve; 123. High-temperature steam inlet pipe for seed transfer valve; 124. Waste steam outlet pipe for seed transfer valve; 125. High-temperature steam inlet pipe for seed return valve; 126. Waste steam outlet pipe for seed return valve; 127. Waste liquid conveying pipe; 128. Steam branch pipe for seed tank inlet pipe; 129. Steam branch pipe for culture tank inlet pipe; 130. Inlet branch pipe;

[0050] 201. High-temperature steam main valve; 202. High-temperature steam inlet valve for air filter; 203. Waste steam exhaust valve for air filter; 204. Seed tank bottom valve; 205. Culture tank bottom valve; 206. Seed return valve; 207. Seed transfer valve; 208. High-temperature steam inlet valve for seed transfer tube; 209. High-temperature steam inlet valve for seed return tube; 210. CIP valve; 211. Steam inlet valve for feeding pneumatic valve; 212. Waste steam exhaust valve for feeding pneumatic valve; 213. Steam inlet valve for acid replenishment pneumatic valve; 214. Waste steam exhaust valve for acid replenishment pneumatic valve; 215. Steam inlet valve for alkali replenishment pneumatic valve; 216. 217. Pneumatic valve for replenishing alkali and exhausting waste steam; 218. Pneumatic valve for replenishing defoaming liquid and steam inlet valve; 219. Pneumatic valve for replenishing defoaming liquid and exhausting waste steam; 220. Discharge valve; 221. Drain valve; 222. CIP discharge valve; 223. Five-way valve; 224. Discharge valve and high-temperature steam inlet valve; 225. Discharge valve and waste steam outlet valve; 226. Transplant valve and high-temperature steam inlet valve; 227. Transplant valve and waste steam outlet valve; 228. Return valve and waste steam outlet valve; 229. Seed tank inlet pipe steam pneumatic valve; 230. Culture tank inlet pipe steam pneumatic valve.

[0051] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0052] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0053] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0054] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0055] This invention introduces a control system for sterilizing a plant tissue culture device, comprising a steam sterilization control module, a plant tissue culture device, a detection device, and a steam delivery pipeline; the steam delivery pipeline delivers steam to the device to be sterilized within the plant tissue culture device, the detection device detects the solution status within the plant tissue culture device, and the steam sterilization control module controls the steam to sterilize the device based on the detection results.

[0056] Specifically, the steam sterilization control module is used to control the steam to sterilize the plant tissue culture device, which is used for plant tissue and cell culture, and is especially suitable for the culture of adventitious roots.

[0057] After receiving information from the detection device installed on the culture device, the steam sterilization control module sends electrical signals to the control valve on the plant tissue culture device and the steam control valve on the steam delivery pipeline to achieve precise sterilization of the device to be sterilized.

[0058] The plant tissue culture device includes a culture tank 9 and a seed tank 8. At least one transplanting tube 6 for conveying plant tissue containing a high concentration from the seed tank 8 to the culture tank 9 and at least one return tube 7 for conveying diluted plant tissue from the culture tank 9 to the seed tank 8 are provided between the culture tank 9 and the seed tank 8.

[0059] like Figure 1 As shown, the steam delivery pipeline includes several steam branch pipes, which are respectively connected to the culture tank 9, seed tank 8, transplanting pipe 6 and return pipe 7. The steam branch pipes are also respectively connected to the devices to be sterilized installed on the culture tank 9, seed tank 8, transplanting pipe 6 and return pipe 7. Each steam branch pipe is equipped with a steam control valve, which is used to receive control commands from the steam sterilization control module and execute the actions of the control commands.

[0060] Specifically, the plant tissue culture apparatus mainly consists of a seed tank 8 for cultivating adventitious root growth and a culture tank 9 for further cultivating the grown adventitious roots. It also includes a transplanting pipe 6 and a return-seeding pipe 7 connecting the two tanks. Both the seed tank 8 and the culture tank are equipped with control valves, as are the steam branch pipes connected to the transplanting pipe 6 and the return-seeding pipe 7. Upon receiving an electrical signal from the steam sterilization control module, the electro-pneumatic valve can perform the corresponding operation. The detection device on the tank for monitoring the solution level is also an electro-controlled device, which can feed back the solution level to the control cabinet, which then sends the electrical signal to the steam sterilization module.

[0061] The steam sterilization control module includes:

[0062] Disinfection control module for transplanting tube, disinfection control module for return tube, disinfection control module for tank pneumatic valve, and disinfection control module for inside tank;

[0063] The steam sterilization control module is a part of the electrical control cabinet. The plant tissue culture device is equipped with multiple control valves. The electrical control cabinet is connected to the control valves on the plant tissue culture device via wiring. The wiring is used to transmit electrical signals for different control commands. The control valves include at least a steam control valve installed on the steam branch pipe and a pneumatic valve for replenishing culture medium into the tank. The steam sterilization control module uses electrical signals to control the steam switches on the steam control valve and the pneumatic valve for replenishing culture medium into the tank to perform opening and closing operations.

[0064] Specifically, the steam sterilization control module can control the control valves connected to all devices in the plant tissue culture apparatus that require sterilization, and can receive electrical signals from the detection device to determine the condition of the solution in the tank. When the solution is out of balance, the detection device sends an electrical signal to the control cabinet, which then automatically performs the corresponding operation upon receiving the signal.

[0065] like Figure 4 , 5As shown in Figure 15, the in-tank disinfection control module is used to control the steam to disinfect the inside of the seed tank 8 and the culture tank 9. Gas distributors 20 are installed inside the culture tank 9 and the seed tank 8. Each gas distributor 20 is connected to an air inlet branch pipe 130. At least two gas distributors 20 are evenly distributed on the bottom wall of the culture device. Each gas distributor 20 is connected to an air inlet branch pipe, and each air inlet branch pipe has the same extension length. The other end of each air inlet branch pipe is connected to a T-connector. The air inlet branch pipe is connected to an air inlet pipe via the T-connector. The air inlet pipe is located near the center of the bottom of the culture tank. The two air inlet branch pipes extend a certain distance to both sides of the tank and bend inward to connect to the gas distributors. The two air inlet branch pipes are symmetrically arranged on the bottom wall. An aeration section 21 is horizontally arranged inside the tank of the gas distributor 20. The aeration section 21 has a cavity at its center, which communicates with the air inlet pipe. The cavity wall has micropores that connect from the outside of the aeration section 21 to the inside of the cavity. A large number of micropores are densely and evenly distributed throughout the cavity wall. The aeration section 21 is made of titanium alloy, which prevents plant tissue from adhering to the outer surface of the aeration section 21, thereby avoiding the accumulation and decay of plant tissue.

[0066] By densely opening a large number of micropores on the side wall of the aeration section 21 and connecting all the micropores to the cavity in the center of the aeration section 21, the gas entering the cavity of the aeration section 21 through the air inlet pipe can be sprayed into the culture medium from all the micropores at the same time. This creates a large number of small bubbles in the culture medium. The large number of continuous small bubbles move outward from the outer surface of the aeration section 21 and float upward, preventing the small bubbles from agglomerating into large bubbles. This creates a driving force for the circulation of the culture medium and plant tissue in the tank, improving the contact and dissolution rate between the gas and the culture medium. Furthermore, the dispersed floating movement of a large number of small bubbles in the culture medium promotes the circulation of the culture medium and further improves the dissolved oxygen effect.

[0067] like Figure 13 As shown, at least two gas distributors 20 and two shearing devices 22 are provided on the bottom wall of the seed tank 8. The shearing devices 22 and the gas distributors 20 are alternately distributed on the bottom wall, and the shearing devices 22 are installed at a position higher than the gas distributors 20 in the vertical direction. The line connecting the installation positions of the two shearing devices 22 and the line connecting the installation positions of the two gas distributors 20 pass through the center of the seed tank 8.

[0068] like Figure 14 As shown, the bottom wall of the culture tank 9 is provided with at least four gas distributors 20 and two shearing devices 22, and the arrangement is similar to that of the seed tank 8.

[0069] Four gas distributors 20 are evenly arranged circumferentially on the bottom wall and are set at equal intervals. The aeration section 21 of the gas distributors 20 is kept horizontal and has a certain length. One end of any two opposing sets of aeration sections 21 connected to the air inlet pipe 19 is spaced from the bottom wall, and the other end is tangent to or intersects the center line of the culture tank. Their projections in the culture tank 9 are approximately parallel and symmetrically arranged with respect to the center of the bioreactor; the projections of two adjacent sets of aeration sections 21 in the culture tank 9 are approximately perpendicular.

[0070] A shearing device 22 is provided at intervals between every two gas distributors 20. The installation position of one shearing device 22 on the same side of the bottom wall and the line connecting the installation positions of the two adjacent gas distributors 20 in the culture tank 9 approximately form an isosceles triangle. The installation position of the shearing device 22 is higher than the gas distributor 20 in the vertical direction.

[0071] The air inlet pipe is connected to a steam branch pipe, and a steam control valve is installed on the steam branch pipe. The in-tank disinfection control module sends an electrical signal to the steam control valve to open the steam control valve, and steam is introduced into the tank through the gas distributor to disinfect the tank.

[0072] like Figure 15 , 16 As shown, by reasonably setting the relative positions of the shearing device 22 and the gas distributor 20, the gas provided by the aeration section 22 of the gas distributor 20 causes the culture medium and plant tissue in the bioreactor to rise along the region near the central axis, then diffuse to the periphery, and then circulate along the trajectory of an outer parabolic surface near the inner wall of the bioreactor. During the process of rising and falling along the trajectory of the near parabolic surface, the culture medium applies a certain force to the plant tissue, causing it to be arranged and flow in a basically parabolic trajectory in the length direction.

[0073] The gas distributor 20 provides air at a certain pressure, causing the culture medium and plant tissue in the bioreactor to rise and fall in a near-parabolic trajectory, and during the descent, they are intermittently sheared by the shearing device 22 and provided with new callus tissue.

[0074] In this embodiment, when the gas distributor 20 supplies gas into the bioreactor, it causes the culture medium and plant tissue to generate a near-parabolic circulating motion. On the one hand, this increases the contact area between the culture medium and plant tissue and the gas, increases the dissolved oxygen rate of the culture medium, and prevents the plant tissue from rotting and deteriorating. On the other hand, the plant tissue can be sheared by the shearing device 22 to avoid entanglement and facilitate discharge. Furthermore, the newly added callus tissue can improve the growth efficiency of the plant tissue, thereby improving the overall growth efficiency and quality of the plant tissue.

[0075] The aeration sections 21 of the two gas distributors 20 of the present invention are preferably arranged on the same plane during installation and their extension directions are approximately parallel in projection within the seed tank 8, and are arranged symmetrically with respect to the center of the bioreactor.

[0076] The aeration section 21 has an extended length, with one end connected to the air inlet pipe and spaced from the bottom wall, and the other end extending towards the center of the bioreactor, tangent to or intersecting the centerline of the bioreactor. The distance between the end of the aeration section 21 connected to the air inlet pipe and the bottom wall is smaller than the distance between the other end of the aeration section 21 and the bottom wall. That is, the length of the air inlet pipe extending into the tank is relatively short, just enough to allow the aeration section 21 to be installed on the bottom wall and its installation angle to be adjusted.

[0077] A single gas distributor 20 can supply both high-temperature steam for sterilization and gas for cultivation into the tank, serving multiple purposes. Furthermore, while providing high-temperature steam for sterilization, the gas distributor 20 also cleans and sterilizes the interior, solving the problem of difficult internal cleaning of traditional gas supply components. Simultaneously, during the sterilization of the tank's interior, the rising steam flow also sterilizes the shearing device.

[0078] The gas distributor 20 provides upward-flowing gas into the seed tank 8 and creates downward turbulence in the culture medium inside the seed tank 8. The turbulence causes the disordered plant tissue to align in the same direction and transports the plant tissue to the incision of the shearing device 3, where it is sheared into small segments.

[0079] The gas distributor 20 is located inside the lower conical sidewall of the tank and is used to replenish gas into the tank. A gas delivery pipeline is connected to the outside of the tank. The gas distributor is a hollow tube with micropores. When sterilization is required, high-temperature steam is released from the micropores of the hollow tube and floats to the surface of the nutrient solution near the conical bottom sidewall of the culture tank, causing the nutrient solution near the sidewall to flow upwards, while the nutrient solution near the center flows downwards. A steam branch pipe connected to the gas delivery pipeline is used to introduce steam into the tank for sterilization. After sterilization, culture medium is added to the tank, and steam is introduced into the tank again through the gas distributor to sterilize the culture medium. The sterilization operation is stopped after the culture medium is heated to the specified temperature and time.

[0080] like Figure 6 As shown, the seed transfer tube disinfection control module is used to control steam to disinfect the inside of the seed transfer tube 6 that transfers seeds from the seed tank 8 to the culture tank 9. The seed transfer tube 6 is connected to a steam branch pipe, and a steam control valve is installed on the steam branch pipe. The seed transfer tube 6 is also equipped with a seed transfer valve 207, which is connected to another steam branch pipe. A steam control valve is also installed on the steam branch pipe of the seed transfer valve 207.

[0081] When transplanting is required, the transplanting tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube 6, and also sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting valve 207 to disinfect the transplanting tube 6 and the transplanting valve 207. After disinfection is completed, the transplanting tube disinfection control module sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube 6 and the steam control valve on the steam branch pipe connected to the transplanting valve 207, and then performs the transplanting operation.

[0082] Specifically, the transplanting tube 6 connects the bottom of the seed tank 8 and the upper side wall of the culture tank 9. When the concentration of adventitious roots in the seed tank 8 reaches a specified value, the detection device sends an electrical signal to the control cabinet. Upon receiving the signal, the control cabinet sends an electrical signal to the transplanting tube disinfection control module. The module controls the high-temperature steam inlet valve 208 on the high-temperature steam inlet pipe 107 connected to the transplanting tube 6 to open, and simultaneously controls the high-temperature steam inlet valve 225 on the high-temperature steam inlet pipe 123 connected to the transplanting valve 207 to open, thus disinfecting the transplanting tube 6 and the transplanting valve 207. After disinfection, the disinfection control module sends a closing electrical signal to the high-temperature steam inlet valve 208 and the high-temperature steam inlet valve 225. Then, the control cabinet sends an opening electrical signal to the bottom valve 204 of the seed tank and the transplanting valve 207 to open them, initiating the transplanting operation.

[0083] like Figure 7 As shown, the return tube disinfection control module is used to control steam to disinfect the inside of the return tube 7 used for returning seeds from the culture tank 9 to the seed tank 8. The return tube 7 is also connected to a steam branch pipe, and a steam control valve is installed on the steam branch pipe. The return tube 7 is also equipped with a return valve 206, which is connected to another steam branch pipe. A steam control valve is also installed on the steam branch pipe of the return valve 206.

[0084] After transplanting, the return seed tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube 7, and also sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return seed valve 206 to disinfect the return seed tube 7 and the return seed valve 206. After disinfection is completed, the return seed tube disinfection control module sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube 7 and the steam control valve on the steam branch pipe connected to the return seed valve 206, and then performs the return seeding operation.

[0085] When the detection device detects that the solution volume in seed tank 8 has decreased to a certain value, it sends an electrical signal to the electrical control cabinet. Upon receiving the signal, the electrical control cabinet first sends a closing signal to the seed transfer valve 207 and the seed tank bottom valve 204, and then sends an electrical signal to the return tube disinfection control module to open the high-temperature steam inlet valve 209 on the high-temperature steam branch inlet pipe 108 connected to the return tube 7. Simultaneously, it controls the opening of the high-temperature steam inlet valve 227 on the high-temperature steam branch inlet pipe 125 connected to the return valve 206, thus disinfecting the return tube 7 and the return valve 206. After disinfection, the return tube disinfection control module sends a closing signal to the high-temperature steam inlet valve 209 and the high-temperature steam inlet valve 227 connected to the return tube. Then, the control cabinet sends electrical signals to the reseeding valve 206 and the bottom valve 205 of the culture tank, opening them and allowing the diluted adventitious root culture solution in the culture tank 9 to flow back into the seed tank 8. When the volume of the solution in the seed tank 8 reaches a certain value, the detection device sends an electrical signal to the control cabinet. Upon receiving the signal, the control cabinet sends a closing signal to the reseeding valve 206 and the bottom valve 205 of the culture tank, closing them.

[0086] like Figure 2 , Figure 8 As shown, the culture tank 9 and the seed tank 8 are equipped with at least one of four pneumatic valves: a feeding pneumatic valve 13, an acid feeding pneumatic valve 14, an alkali feeding pneumatic valve 15, and an antifoaming liquid feeding pneumatic valve 16. The tank body pneumatic valve disinfection control module is used to control steam to disinfect the pneumatic valves. The tank body is also equipped with a detection device for detecting at least one of the pH value, solution concentration, and foam density in the tank. The detection device is connected to the electrical control cabinet via a line.

[0087] When the detection device detects an imbalance in the solution, it sends an electrical signal to the electrical control cabinet. Upon receiving the signal, the electrical control cabinet sends an electrical signal to the tank pneumatic valve control module to indicate the required replenishment of liquid. The tank pneumatic valve control module then sends an electrical signal to the pneumatic valve containing the liquid to be replenished, controlling the opening of the steam switch on the pneumatic valve to introduce steam for sterilization. After sterilization is complete, the tank pneumatic valve control module sends an electrical signal to the pneumatic valve to close the steam switch, and then the liquid replenishment operation is performed.

[0088] The feeding pneumatic valve 13 is used to replenish the culture medium into the tank. The acid replenishing pneumatic valve 14 and the alkali replenishing pneumatic valve 15 are used to replenish acid and alkali solutions into the tank to maintain the pH value. The defoaming liquid replenishing pneumatic valve 16 is used to replenish defoaming liquid into the tank to eliminate foam generated by adventitious roots in the tank. The pneumatic valves used to replenish materials into the tank need to be disinfected before each use to prevent contamination of the solution by introducing contaminants and pathogens into the culture tank during the replenishment process.

[0089] The detection device is located in the middle of the tank. It uses electrodes to detect changes in the solution inside the tank and sends electrical signals to the control cabinet for feedback. The electrodes of the detection device are immersed in the culture medium, and the components in the sample are detected through the electrochemical reaction between the electrodes and the culture medium. When a decrease in the concentration of the solution in the tank is detected, culture medium needs to be added; when the pH value of the solution in the tank is detected to be lower than the preset value, alkali solution needs to be added; when the pH value of the solution in the tank is detected to be higher than the preset value, acid solution needs to be added; when the density of foam in the tank is detected to be too high, defoaming solution needs to be added.

[0090] The present invention also provides a method for controlling the sterilization of a plant tissue culture device, comprising the following steps:

[0091] S1, such as Figure 9 As shown, culture medium is added into seed tank 8. The disinfection control module inside the tank sends an opening electrical signal to the steam control valve connected to the gas distributor inside seed tank 8 to disinfect the culture medium inside seed tank 8. After disinfection, the disinfection control module inside the tank sends an electrical signal to the steam control valve to close the steam control valve and adds adventitious roots into seed tank 8 for a certain period of time.

[0092] S2, such as Figure 10 As shown, after the adventitious roots are cultured in the seed tank 8 for a period of time, culture medium is added to the culture tank 9. The disinfection control module inside the tank sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the gas distributor inside the culture tank 9 to perform steam disinfection of the culture medium inside the culture tank 9.

[0093] S3, such as Figure 11 As shown, after the culture medium in the culture tank 9 is disinfected, the transplanting tube disinfection control module sends an electrical signal to the steam pneumatic valve on the steam branch pipe connected to the transplanting tube 6 to open the steam switch on the steam pneumatic valve to disinfect the inside of the transplanting tube 6. At the same time, the transplanting tube disinfection control module sends an electrical signal to the transplanting valve 207 to open the steam switch on the transplanting valve 207 to disinfect the transplanting valve. After disinfection, the adventitious root culture medium in the seed tank 8 is transferred to the culture tank 9 for cultivation.

[0094] Specifically, when the steam sterilization control module sterilizes the plant tissue culture device, it first controls the opening of the seed tank inlet steam pneumatic valve 229 on the seed tank inlet steam branch pipe 128 connected to the seed tank inlet pipe 18 via the in-tank sterilization control module. Steam is then introduced into the seed tank 8 through the gas distributor inside the seed tank 8 to sterilize the culture medium added to the seed tank 8. Adventitious roots are then added to the seed tank 8 for cultivation. During cultivation, a cutting unit is installed at the bottom of the tank to cut the grown adventitious roots to a certain length. A detection device on the tank constantly monitors the solution inside. When the solution concentration in the tank becomes unbalanced, the detection device transmits an electrical signal back to the main control unit of the electrical control cabinet. The steam sterilization control module in the electrical control cabinet sends an electrical signal to the pneumatic valve on the tank body used to replenish the material solution, controlling the opening of the steam sterilization switch on the pneumatic valve to sterilize the pneumatic valve. After sterilization, the required replenishing material solution is added to the tank.

[0095] When the adventitious roots in seed tank 8 reach a certain concentration, they need to be transferred to culture tank 9. Culture tank 9 is larger than seed tank 8 and can accommodate more adventitious roots for cultivation. Transferring the adventitious roots to culture tank 9 is done through transfer tube 6. Before transfer, culture medium needs to be added to culture tank 9, and the in-tank sterilization control module in the steam sterilization control module also needs to control steam to sterilize the culture medium in culture tank 9. After the culture medium in the culture tank is sterilized, the transfer tube sterilization control module controls steam to sterilize transfer tube 6, and then the adventitious root culture solution in seed tank 8 is introduced into culture tank 9 for cultivation.

[0096] like Figure 12 As shown, after step S3, it is necessary to transfer the seed culture from the culture tank 9 to the seed tank 8. The seed return tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the seed return tube 7 to disinfect the inside of the seed return tube 7. The seed return tube disinfection control module then sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the seed return valve 206 to disinfect the seed return valve. After disinfection, the diluted adventitious root culture solution in the culture tank 9 is returned to the seed tank 8 for continued cultivation.

[0097] Specifically, to reduce the risk of contamination from manual feeding, this system includes a return seed tube 7 between the two tanks. After the adventitious root culture medium is completely introduced into the culture tank 9, the return seed tube steam sterilization control module sends an electrical signal to the return seed tube high-temperature steam inlet valve 209 on the return seed tube high-temperature steam branch inlet pipe 108 connected to the return seed tube 7 to control steam to enter the return seed tube for sterilization. After sterilization, the diluted adventitious root culture medium in the culture tank is then returned to the seed tank through the return seed tube to continue cultivating adventitious roots.

[0098] In steps S1 and S2, after adding culture medium to the seed tank 8 and culture tank 9, the culture medium inside the tank needs to be sterilized. Steam is introduced into the tank through a gas distributor to heat the culture medium inside the tank to the specified temperature and time, thus completing the sterilization of the culture medium.

[0099] Specifically, the culture medium added to the tank inevitably carries the risk of contamination with pathogens. Therefore, after adding the culture medium to the tank, it is necessary to introduce steam for high-temperature sterilization to maintain the culture medium in a sterile state. The detection device on the tank can detect the temperature of the culture medium inside and send an electrical signal to the main control unit in the electrical control cabinet. The sterilization control module inside the tank sends an electrical signal to the steam pneumatic valve on the steam branch pipe connected to the gas distributor to regulate and control the steam flow rate into the tank, ensuring that the temperature for sterilizing the culture medium is within a reasonable range. The sterilization time must also be controlled to avoid excessively high temperatures and prolonged times leading to culture medium inactivation, or excessively low temperatures and insufficient time resulting in incomplete sterilization.

[0100] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A control system for disinfecting a plant tissue culture device, characterized in that, include: The system includes a steam sterilization control module, a plant tissue culture device, a detection device, and a steam delivery pipeline. The steam delivery pipeline delivers steam to the plant tissue culture device to be sterilized. The detection device detects the solution status within the plant tissue culture device. The steam sterilization control module controls the steam to sterilize the device based on the detection results. The plant tissue culture device includes a culture tank and a seed tank. The steam sterilization control module includes an in-tank sterilization control module, which is used to control the steam to sterilize the inside of the seed tank and the culture tank. The culture tank and seed tank are equipped with a gas distributor, which is located inside the lower conical side wall of the tank. The gas distributor has an aeration section horizontally arranged inside the tank. The center of the aeration section has a cavity, which is connected to the air inlet pipe. The cavity wall has micropores that connect from the outside of the aeration section to the cavity. A large number of micropores are densely and evenly distributed throughout the cavity wall. At least four gas distributors are provided on the bottom wall of the culture tank. The four gas distributors are evenly distributed around the circumference of the bottom wall and are set at equal intervals. The aeration section of the gas distributor is kept horizontal and has a certain length. One end of any two opposite aeration sections connected to the air inlet pipe is spaced from the bottom wall, and the other end is tangent to or intersects the center line of the culture tank. Their projections in the culture tank are approximately parallel and symmetrically arranged with respect to the center of the bioreactor. The projections of two adjacent aeration sections in the culture tank are approximately perpendicular. Two shearing devices are installed on the bottom wall of the culture tank; a shearing device is installed between every two gas distributors. The installation position of one shearing device on the same side of the bottom wall and the installation positions of the two adjacent gas distributors form an approximately isosceles triangle inside the culture tank. The installation position of the shearing device is higher than the gas distributor in the vertical direction.

2. The control system for disinfecting a plant tissue culture device according to claim 1, characterized in that, At least one transplanting tube (6) for conveying a high concentration of plant tissue from the seed tank (8) to the culture tank (9) and at least one return tube (7) for returning diluted plant tissue from the culture tank (9) to the seed tank (8) are provided between the culture tank (9) and the seed tank (8); The steam delivery pipeline includes several steam branch pipes, which are respectively connected to the culture tank (9), seed tank (8), transplanting pipe (6) and return pipe (7). The steam branch pipes are also respectively connected to the devices to be sterilized set on the culture tank (9), seed tank (8), transplanting pipe (6) and return pipe (7). Each steam branch pipe is equipped with a steam control valve, which is used to receive control instructions from the steam sterilization control module and execute the actions of the control instructions.

3. The control system for disinfecting a plant tissue culture device according to claim 1, characterized in that, The steam sterilization control module also includes: a seed transfer tube sterilization control module, a seed return tube sterilization control module, and a tank pneumatic valve sterilization control module; The steam sterilization control module is a part of the electrical control cabinet. The plant tissue culture device is equipped with multiple control valves. The electrical control cabinet is connected to the control valves on the plant tissue culture device via wiring. The wiring is used to transmit electrical signals for different control commands. The control valves include at least a steam control valve installed on the steam branch pipe and a pneumatic valve for replenishing culture medium into the tank. The steam sterilization control module uses electrical signals to control the steam switches on the steam control valve and the pneumatic valve for replenishing culture medium into the tank to perform opening and closing operations.

4. A control system for disinfecting a plant tissue culture device according to claim 3, characterized in that, The gas distributor (20) is connected to an air inlet branch pipe. Each air inlet branch pipe has the same extension length. The other end of each air inlet branch pipe is connected to a three-way pipe. The air inlet branch pipe is connected to the air inlet pipe through the three-way pipe. The air inlet pipe is located near the center of the bottom of the culture tank. Two air inlet branch pipes extend a certain distance to both sides of the tank and bend inward to connect to the gas distributor. The two air inlet branch pipes are symmetrically arranged on the bottom wall. A steam branch pipe is connected to the air inlet pipe. A steam control valve is installed on the steam branch pipe. The tank disinfection control module sends an electrical signal to the steam control valve to open the steam control valve and introduce steam into the tank through the gas distributor (20) to disinfect the tank.

5. A control system for disinfecting a plant tissue culture device according to claim 3, characterized in that, The seed tube disinfection control module is used to control steam to disinfect the inside of the seed tube (6) that transfers seeds from the seed tank (8) to the culture tank (9). The seed tube (6) is connected to a steam branch pipe, and a steam control valve is installed on the steam branch pipe. The seed tube (6) is also equipped with a seed transfer valve (207), which is connected to another steam branch pipe. A steam control valve is also installed on the steam branch pipe of the seed transfer valve (207). When transplanting is required, the transplanting tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube (6), and also sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplanting valve (207) to disinfect the transplanting tube (6) and the transplanting valve (207). After disinfection is completed, the transplanting tube disinfection control module sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the transplanting tube (6) and the steam control valve on the steam branch pipe connected to the transplanting valve (207), and then performs the transplanting operation.

6. A control system for disinfecting a plant tissue culture device according to claim 5, characterized in that, The disinfection control module for the return tube is used to control steam to disinfect the inside of the return tube (7) used for returning seeds from the culture tank (9) to the seed tank (8). The return tube (7) is also connected to a steam branch pipe, and a steam control valve is provided on the steam branch pipe. The return tube (7) is also equipped with a return valve (206), and another steam branch pipe is connected to the return valve (206). A steam control valve is also provided on the steam branch pipe of the return valve (206). After transplanting, the disinfection control module of the return seed tube sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube (7), and also sends an opening electrical signal to the steam control valve on the steam branch pipe of the return seed valve (206) to disinfect the return seed tube (7) and the return seed valve (206). After disinfection, the disinfection control module of the return seed tube sends a closing electrical signal to the steam control valve on the steam branch pipe connected to the return seed tube (7) and the steam control valve on the steam branch pipe connected to the return seed valve (206), and then performs the return seeding operation.

7. A control system for disinfecting a plant tissue culture device according to claim 2 or 3, characterized in that, The culture tank (9) and seed tank (8) are equipped with at least one of four pneumatic valves: a feeding pneumatic valve (13), an acid feeding pneumatic valve (14), an alkali feeding pneumatic valve (15), and an antifoaming liquid feeding pneumatic valve (16). The tank body pneumatic valve disinfection control module is used to control steam to disinfect the pneumatic valves. The tank body is also equipped with a detection device for detecting at least one of the pH value, solution concentration, and foam density in the tank. The detection device is connected to the electrical control cabinet via a line. When the detection device detects an imbalance in the solution, it sends an electrical signal to the electrical control cabinet. Upon receiving the signal, the electrical control cabinet sends an electrical signal to the tank pneumatic valve control module to indicate the required replenishment of liquid. The tank pneumatic valve control module then sends an electrical signal to the pneumatic valve containing the liquid to be replenished, controlling the opening of the steam switch on the pneumatic valve to introduce steam for sterilization. After sterilization is complete, the tank pneumatic valve control module sends an electrical signal to the pneumatic valve to close the steam switch, and then the liquid replenishment operation is performed.

8. A method for controlling the disinfection of a plant tissue culture device, characterized in that, The control system for sterilizing a plant tissue culture device, as described in any one of claims 1-7, is used. The control method includes the following steps: S1. Add culture medium into the seed tank (8). The disinfection control module in the tank sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the gas distributor in the seed tank (8) to disinfect the culture medium in the seed tank (8). After disinfection, the disinfection control module in the tank sends an electrical signal to the steam control valve to close the steam control valve and adds plant tissue culture into the seed tank (8) for a certain period of time. S2. After the plant tissue has been cultured in the seed tank (8) for a period of time, culture medium is added to the culture tank (9). The disinfection control module in the tank sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the gas distributor in the culture tank (9) to perform steam disinfection on the culture medium in the culture tank (9). S3. After the culture medium in the culture tank (9) is disinfected, the transplant tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplant tube (6) to disinfect the inside of the transplant tube (6). At the same time, the transplant tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the transplant valve (207) to disinfect the transplant valve (207). After disinfection, the plant tissue culture medium in the seed tank (8) is transferred to the culture tank (9) for cultivation.

9. A method for controlling the disinfection of a plant tissue culture device according to claim 8, characterized in that, After step S3, step S4 is also included: the return tube disinfection control module sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return tube (7) to disinfect the inside of the return tube (7), and the return tube disinfection control module then sends an opening electrical signal to the steam control valve on the steam branch pipe connected to the return valve (206) to disinfect the return valve (206). After disinfection, the diluted part of the plant tissue culture solution in the culture tank (9) is returned to the seed tank (8) for continued culture.

10. A method for controlling the disinfection of a plant tissue culture device according to claim 8, characterized in that, In steps S1 and S2, after adding culture medium to the seed tank (8) and culture tank (9), the culture medium in the tank is sterilized. Steam is introduced into the tank through a gas distributor to heat the culture medium in the tank to the specified temperature and time, thus completing the sterilization of the culture medium.

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