A pesticidal composition for controlling tobacco budworm

Abstract

The application belongs to the technical field of pesticides, and particularly relates to a kind of insecticidal composition for preventing and treating tobacco budworms. The effective component of the insecticidal composition is compounded by sulfoxaflor, flupyradifurone, spinetoram or chromafenozide. The application compounds sulfoxaflor with existing insecticidal components, and screens out a suitable compound formula. Within a certain mass ratio range, the insecticidal composition shows a synergistic effect on tobacco budworms, and can provide support for developing alternative pesticides for preventing and treating tobacco budworms.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically to an insecticidal composition for controlling tobacco budworm. Background Technology

[0002] The tobacco budworm, also known as the tobacco noctuid moth, is an agricultural pest belonging to the family Noctuidae in the order Lepidoptera. It is a global pest that can damage a variety of plants and vegetables, with tobacco and chili peppers being the most severely affected. The larvae of the tobacco budworm feed on the new shoots and tender leaves of tobacco plants, causing a decline in tobacco yield and quality.

[0003] For a long time, chemical control of tobacco budworms has mainly relied on organophosphates, carbamates, and pyrethroids, leading to varying degrees of pesticide resistance in the budworms and reduced control efficacy. In some tobacco-growing areas, the excessive use of chemical pesticides to improve pest control has caused serious environmental pollution, reduced tobacco quality, and exacerbated the development of pesticide resistance in tobacco budworms, hindering the sustainable development of the tobacco industry. Therefore, there is an urgent need to screen for highly effective, low-residue novel insecticides as alternatives for controlling tobacco budworms in tobacco production.

[0004] Thiofofluorooxime ether is a non-ester oxime ether compound with independent intellectual property rights, created by the Hunan Chemical Research Institute. Molecular formula: C 23 H 21 ClFNOS, with the following structural formula:

[0005]

[0006] Thiofop-oxime exhibits strong stomach poison and contact action against insects, making it suitable for controlling various lepidopteran and homoptera insects on crops. It also exhibits low toxicity to humans and livestock and is safe for crops. While thiofluop-oxime possesses these advantages, long-term use as a single pesticide will inevitably lead to the development and progression of pesticide resistance in pests. Rational combination of thiofluop-oxime with existing pesticides can reduce the risk of resistance and improve the control efficacy against target pests. Currently, there are no reports of thiofluop-oxime being combined with flupyrflurane, imidacloprid, or cyclooxygenated.

[0007] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0008] The purpose of this invention is to provide an insecticidal composition for controlling tobacco budworm, which can improve the control efficacy, reduce the amount of pesticides used, and help ensure tobacco yield and quality, as well as the sustainable development of the tobacco industry.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] An insecticidal composition, wherein the active ingredient of the insecticidal composition is a compound of thioflufenoxam, flupyrrolidone, pipemidone, or cyclopyridoxine.

[0011] Preferably, the mass ratio of the thiofluoxime ether to flupyrofurone is 1-7:20-1.

[0012] Preferably, the mass ratio of thioflume to piperazine is 1-19:19-1.

[0013] Preferably, the mass ratio of the thioflume to cyclophosphamide is 1-25:5-1.

[0014] The present invention also provides the application of the insecticidal composition in the control of tobacco pests.

[0015] Preferably, the tobacco pests include tobacco budworms.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) This invention combines thioflume ether with existing insecticidal ingredients, selects suitable compound formulations, and shows a synergistic effect on tobacco budworm within a certain mass ratio range, improves control efficacy, reduces pesticide application, and helps ensure tobacco yield and quality, as well as the sustainable development of the tobacco industry.

[0018] (2) The present invention combines thioflumezine with other components in a reasonable manner, which can reduce the risk of resistance to thioflumezine and improve the control effect on target pests, and can provide support for the development of alternative agents for the control of tobacco budworm. Detailed Implementation

[0019] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0020] Example Indoor bioactivity test

[0021] 1. Test subjects

[0022] Tobacco budworms were collected from the flue-cured tobacco growing area in Baise City, Guangxi Zhuang Autonomous Region. After being reared for six generations in the laboratory of Guangxi University, healthy second-instar larvae with basically the same body size were selected as experimental subjects.

[0023] 2. Test reagents

[0024] 95% thiophanate-methyl technical (Hunan Haili Chemical Co., Ltd.), 96% flupyrrolidone technical (Bayer AG), 95% piperamididine technical (Jiangsu Kesheng Group Co., Ltd.), 95% cyclooxygenated technical (Sichuan Hebang Biotechnology Co., Ltd.).

[0025] The original drug was dissolved in dimethyl sulfoxide and then diluted with a 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple formulations were prepared, and five mass concentration gradients were set for each single-dose stock solution and each formulation mixture according to the same ratio method. Each mass concentration solution was 50 mL and ready for use.

[0026] 3. Test Methods

[0027] The leaf immersion method was used. Tobacco leaves containing second-instar budworm larvae (the larvae had been starved for 5 hours) were immersed in the pesticide solution for 10 seconds, removed, air-dried, and placed in 9cm diameter petri dishes. The bottom of the dishes was lined with filter paper to maintain moisture, and the mouths were sealed with double layers of damp gauze. One tobacco leaf containing a budworm was placed in each petri dish. Each concentration of pesticide solution treated 20 larvae, with three replicates. A 0.1% Tween-80 solution treatment was included as a blank control. The experimental larvae were further reared in an artificial climate chamber at 27℃ and 80% relative humidity, with a light / dark cycle of 18h / 6h. After 48h, the experimental larvae were checked; those that did not move when touched with a pen tip were considered dead. The total number of larvae and the number of dead larvae were recorded for each treatment, and the corrected mortality rate for each treatment was calculated.

[0028]

[0029] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0030]

[0031] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0032] 4. Data Analysis

[0033] Regression analysis was performed on the logarithmic values ​​of drug concentrations and corrected mortality odds to calculate the LC50 of each treatment. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method, and the synergistic effect of the drug was evaluated based on the calculated CTC value. The results are shown in Table 1-3.

[0034] Table 1. Results of in vitro bioactivity assays of the combination of thiofluridine and flupyrrolidone on tobacco budworms.

[0035] Drug Name and Proportion LC50 (mg / L) ATI TTI CTC thiofluoxime ether 12.6947 100.0000 -- -- Flupyrrolidone 3.4712 365.7150 -- -- Thiofoxime 1: Flupyrrolidone 20 2.3176 547.7520 353.0619 155.1433 Thiofoxime 1: Flupyrrolidone 15 3.0167 420.8141 349.1078 120.5399 Thiofoxime 1: Flupyrrolidone 7 1.9611 647.3255 332.5006 194.6840 Thiofoxime 1: Flupyrrolidone 3 3.2747 387.6599 299.2863 129.5281 Thiofoxime 1: Flupyrrolidone 1 4.1369 306.8650 232.8575 131.7823 Thiofoxime 3: Flupyrrolidone 1 5.6032 226.5616 166.4288 136.1313 Thiofoxime 7: Flupyrrolidone 1 7.5008 169.2446 133.2144 127.0468

[0036] Table 1 shows that when thioflumezine and flupyrrolidone are combined, within a mass ratio of 1-7:20-1, the co-toxicity coefficients against tobacco budworms for each combination are greater than 120, exhibiting a synergistic effect. This indicates that the combination of thioflumezine and flupyrrolidone can improve the control effect against tobacco budworms.

[0037] Table 2. Results of in vitro bioactivity assays of the combination of thioflufenican and piperazine on tobacco budworm.

[0038]

[0039]

[0040] Table 2 shows that when thioflumezine and piperazine are combined, within a mass ratio of 1-19:19-1, the co-toxicity coefficients against tobacco budworm in each combination are greater than 120, exhibiting a synergistic effect. This indicates that the combination of thioflumezine and piperazine can improve the control effect against tobacco budworm.

[0041] Table 3. Results of indoor bioactivity assays of the combination of thioflufenican and cyclophosphamide on tobacco budworm.

[0042] Drug Name and Proportion LC50 (mg / L) ATI TTI CTC thiofluoxime ether 12.6947 100.0000 -- -- Epoxypyr 6.2165 204.2098 -- -- Thioflume 1: Epoxypyr 5 1.7396 729.7482 186.8415 390.5708 Thiofoxime 1: Epioxuron 3 2.9034 437.2357 178.1573 245.4211 Thiofoxime 1: Epioxuron 1 6.1112 207.7284 152.1049 136.5692 Thiofoxime 3: Epioxuron 1 2.2857 555.3966 126.0524 440.6076 Thioflume 5: Epioxuron 1 5.4735 231.9302 117.3683 197.6089 Thiofoxime 15: Epioxime 1 7.2616 174.8196 106.5131 164.1297 Thioflume 25: Epioxuron 1 3.1946 397.3800 104.0081 382.0665

[0043] Table 3 shows that when thioflumezine and cyclophosphamide are combined, within a mass ratio of 1-25:5-1, the co-toxicity coefficients against tobacco budworm are all greater than 120, exhibiting a synergistic effect. This indicates that the combination of thioflumezine and cyclophosphamide can improve the control effect against tobacco budworm.

[0044] In summary, when thioflumimonde is combined with flupyrrolidone, imidacloprid, or cyclooxygenated chlorpyrifos in this invention, it exhibits a synergistic effect against tobacco budworm within a certain mass ratio range, improving control efficacy, reducing pesticide application, and helping to ensure tobacco yield and quality, as well as the sustainable development of the tobacco industry.

[0045] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. An insecticidal composition, characterized by comprising, The active ingredient of the insecticidal composition is a compound of thioflumezine and cyclophosphamide, wherein the mass ratio of thioflumezine to cyclophosphamide is 1-25:5-1.

2. The application of the insecticidal composition according to claim 1 in the control of tobacco pests.

3. Use according to claim 2, characterized in that, The tobacco pests include tobacco budworms.

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