Method for rapidly treating traditional Chinese medicine wastewater by using activated carbon immobilized enzyme

Abstract

The present application relates to the technical field of traditional Chinese medicine wastewater treatment, and specifically discloses a method for rapidly treating traditional Chinese medicine wastewater by using activated carbon immobilized enzyme, which comprises the following steps: activated carbon selection, activated carbon pretreatment, immobilized alkaline xylanase, activated carbon with immobilized enzyme treating traditional Chinese medicine wastewater, and filtering the water treated by activated carbon with immobilized enzyme; ordinary medium-speed filter paper is used for preliminary filtration to remove larger impurities and particles; then, a filter membrane with a pore size of 0.22-0.45 um is used for further filtration to remove smaller particles and impurities; and finally, the treated water is obtained. The present application can efficiently degrade natural organic polysaccharide pollutants in traditional Chinese medicine wastewater by using activated carbon immobilized enzyme, improve the biodegradability of wastewater, reduce membrane blockage, reduce operation cost, efficiently reduce the colority, COD, total nitrogen and ammonia nitrogen of the treated water, and thus simplify the traditional Chinese medicine wastewater treatment process and reduce the operation cost of a wastewater treatment plant.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine wastewater treatment technology, specifically relating to a method for rapidly treating traditional Chinese medicine wastewater using activated carbon-immobilized enzymes. Background Technology

[0002] Traditional Chinese medicine (TCM) wastewater contains a high proportion of cellulose and hemicellulose, and has a high color intensity. Wastewater treatment plants face problems such as heavy load, high cost, and difficulty in achieving effluent standards. Currently, the main technologies for treating TCM wastewater include: bar filtration, anaerobic reaction, aeration treatment, iron-carbon micro-electrolysis, coagulation sedimentation, advanced oxidation, and MBR membrane filtration.

[0003] MBR membrane filtration technology, widely used in wastewater treatment plants, effectively removes various pollutants. However, the membranes are easily clogged by natural organic matter, making cleaning and replacement troublesome and resulting in high operating costs. Soluble polysaccharides with molecular weights exceeding one million can still pass through 0.22µm pore size MBR membranes and easily adhere to the membrane pores, causing blockage. These permeate polysaccharides also affect the color and COD control of the effluent. This further complicates the treatment of traditional Chinese medicine wastewater and increases the operating costs of treatment plants. Summary of the Invention

[0004] The purpose of this invention is to provide a method for rapidly treating traditional Chinese medicine wastewater using activated carbon-immobilized enzymes, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A method for rapidly treating traditional Chinese medicine wastewater using activated carbon-immobilized enzymes includes the following steps:

[0007] S1. Selection of activated carbon: Select wood-based activated carbon with a pore size of 32-100 mesh;

[0008] S2, Activated carbon pretreatment: using 0.2-1.0 mol·L⁻¹ -1 Soak the wood-based activated carbon in hydrochloric acid solution for 3-12 hours, wash until neutral, and then use 0.4-2 mol·L⁻¹ hydrochloric acid solution. -1 Soak in sodium hydroxide for 3-12 hours, wash until neutral, centrifuge to remove liquid, spread evenly in a container, and then dry at 50-100℃ for 1.5-3 hours;

[0009] S3. Immobilized alkaline xylanase: Prepare alkaline xylanase at a concentration of 10-220 μg / mL using pH 8.0 hydrochloric acid-Tris buffer. -1 Add 5-25 mg of treated activated carbon to 4-20 mL of the solution, stir magnetically for 1-4 h, rinse until neutral, filter with filter paper, air dry at low temperature, and use immediately, or soak in hydrochloric acid-Tris buffer and store at 4°C for later use.

[0010] S4. Treatment of traditional Chinese medicine wastewater with immobilized enzyme activated carbon: Ensure treatment temperature of 20-60℃, treatment time of 1-24h, immobilized enzyme activated carbon content of 0.01%-1%, and magnetic stirring at 50-100r / min.

[0011] S5. Filtration of water treated with immobilized enzyme activated carbon: Let the mixture from S4 stand for 5-20 minutes. First, use ordinary medium-speed filter paper for preliminary filtration to remove larger impurities and particles. Then, use a filter membrane with a pore size of 0.22-0.45um for further filtration to remove smaller particles and impurities, resulting in the final treated water.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] This invention utilizes activated carbon to immobilize enzymes to efficiently degrade natural organic polysaccharide pollutants in traditional Chinese medicine wastewater, thereby improving the biodegradability of the wastewater, reducing membrane clogging, lowering operating costs, and effectively reducing the color, COD (chemical oxygen demand), total nitrogen, and ammonia nitrogen in the treated water. This simplifies the treatment process of traditional Chinese medicine wastewater and reduces the operating costs of wastewater treatment plants. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the process of the present invention;

[0015] Figure 2 This is a bar graph showing the relationship between alkaline xylanase concentration and enzyme loading in this invention.

[0016] Figure 3 This is a bar graph showing the relationship between alkaline xylanase concentration and enzyme activity retention in this invention.

[0017] Figure 4 This is a bar graph showing the relationship between the concentration of enzyme-loaded activated carbon in this invention and the removal rates of total nitrogen, ammonia nitrogen, COD, and color. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1:

[0020] Please see Figure 1 - Figure 4 As shown, a method for rapidly treating traditional Chinese medicine wastewater using activated carbon-immobilized enzymes includes the following steps:

[0021] Select wood-based activated carbon with a pore size of 100 mesh, and use 0.5 mol·L⁻¹ -1 Soak in hydrochloric acid solution for 4 hours, wash until neutral, and then use 1 mol·L⁻¹ water. -1 Soak in sodium hydroxide for 4 hours, wash until neutral, centrifuge to remove liquid, spread evenly in a container, dry at 100℃ for 2 hours, and prepare alkaline xylanase at 100 μg / mL using pH 8.0 hydrochloric acid-Tris buffer. -1 Add 5 mg of treated activated carbon to 4 mL of the solution, stir magnetically for 4 h, rinse until neutral, filter with filter paper, air dry at low temperature, soak in hydrochloric acid-Tris buffer, and store at 4℃ for later use. When treating traditional Chinese medicine wastewater with immobilized enzyme activated carbon, the treatment temperature is 55℃, the treatment time is 1 h, the immobilized enzyme activated carbon content is 0.5%, and the magnetic stirring is 60 r·min. -1 The stirred mixture was allowed to stand for 10 minutes. The supernatant was then filtered through ordinary medium-speed filter paper to remove larger impurities and particulate matter, and changes in various pollution indicators were monitored. Then, further filtration was performed using a 0.45µm filter membrane to remove even smaller particles and impurities, ultimately yielding treated water.

[0022] Tests showed that the water treated by filter paper had a color removal rate of 96%, a COD removal rate of 85%, a total nitrogen removal rate of 38%, and an ammonia nitrogen removal rate of 83%.

[0023] Example 2:

[0024] Please see Figure 1 - Figure 4 As shown, wood-based activated carbon with a pore size of 100 mesh was selected, and 0.5 mol·L⁻¹ was used. -1 Soak in hydrochloric acid solution for 4 hours, wash until neutral, and then use 1 mol·L⁻¹ water. -1 Soak in sodium hydroxide for 4 hours, wash until neutral, centrifuge to remove liquid, spread evenly in a container, dry at 100℃ for 2 hours, and prepare alkaline xylanase at 100 μg / mL using pH 8.0 hydrochloric acid-Tris buffer. -1 Add 5 mg of treated activated carbon to 4 mL of the solution, stir magnetically for 4 h, rinse until neutral, filter with filter paper, air dry at low temperature, soak in hydrochloric acid-Tris buffer, and store at 4℃ for later use. When treating traditional Chinese medicine wastewater with immobilized enzyme activated carbon, the treatment temperature is 55℃, the treatment time is 1 h, the immobilized enzyme activated carbon content is 1%, and the magnetic stirring is 60 r·min. -1 The stirred mixture was allowed to stand for 10 minutes. The supernatant was then filtered through ordinary medium-speed filter paper to remove larger impurities and particulate matter, and changes in various pollution indicators were monitored. Then, further filtration was performed using a 0.45µm filter membrane to remove even smaller particles and impurities, ultimately yielding treated water.

[0025] Tests showed that the water treated by filter paper achieved a color removal rate of 97.02%, a COD removal rate of 85.7%, a total nitrogen removal rate of 48.32%, and an ammonia nitrogen removal rate of 84.7%.

[0026] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for rapidly treating traditional Chinese medicine wastewater using activated carbon-immobilized enzymes, characterized in that, Includes the following steps: S1. Selection of activated carbon: Select wood-based activated carbon with a pore size of 32-100 mesh; S2, Activated carbon pretreatment: using 0.2-1.0 mol·L⁻¹ -1 Soak the wood-based activated carbon in hydrochloric acid solution for 3-12 hours, wash until neutral, and then rinse with 0.4-2 mol·L⁻¹ hydrochloric acid solution. -1 Soak in sodium hydroxide for 3-12 hours, wash until neutral, centrifuge to remove liquid, spread evenly in a container, and then dry at 50-100℃ for 1.5-3 hours; S3. Immobilized alkaline xylanase: Prepare alkaline xylanase at a concentration of 10-220 μg / mL using pH 8.0 hydrochloric acid-Tris buffer. -1 Add 5-25 mg of the prepared activated carbon to 4-20 mL of the solution, stir magnetically for 1-4 h, rinse until neutral, filter with filter paper, air dry at low temperature and use immediately. For storage, soak in hydrochloric acid-Tris buffer and store at 4°C. S4. Treatment of traditional Chinese medicine wastewater with immobilized enzyme activated carbon: Ensure treatment temperature of 20-60℃, treatment time of 1-24 h, immobilized enzyme activated carbon content of 0.01%-1%, and magnetic stirring at 50-100 r / min. S5. Filtration of water treated with activated carbon and immobilized enzymes: After stirring in S4, the mixture is allowed to stand for 5-20 minutes. First, it is initially filtered using ordinary medium-speed filter paper to remove larger impurities and particles. Then, it is further filtered using a filter membrane with a pore size of 0.22-0.45 μm to remove smaller particles and impurities, finally obtaining the treated water. By utilizing activated carbon immobilized enzymes to efficiently degrade natural organic polysaccharide pollutants in traditional Chinese medicine wastewater, the biodegradability of the wastewater is improved, membrane clogging is reduced, operating costs are lowered, and the color, COD, total nitrogen, and ammonia nitrogen of the treated water are effectively reduced.

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