Compositions with dual moisturizing and repairing effects, inclusion liposomes and their preparation methods, composite compositions and their applications

Abstract

This invention provides a composition with dual moisturizing and repairing effects, an inclusion liposome, a method for preparing the same, a composite composition, and its application. The composition with dual moisturizing and repairing effects includes acetylglucosamine, adenosine, and sialic acid. Acetylglucosamine, adenosine, and sialic acid each have moisturizing and repairing effects, and their combined use can synergistically enhance their effects, resulting in significant moisturizing and repairing efficacy. Compared to the weak moisturizing and repairing effects when using any one or two of the above three substances, the composition simultaneously including acetylglucosamine, adenosine, and sialic acid shows a significant improvement in the effects of hydration, moisturizing, and skin repair.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, and in particular to a composition with dual moisturizing and repairing effects, an inclusion liposome and its preparation method, a composite composition and its application. Background Technology

[0002] With increasing emphasis on skincare and a growing demand for healthy skin, the demand for high-quality moisturizing skincare products is also rising. Currently, most moisturizing products suffer from various shortcomings. For example, moisturizing lotions may have limited functionality and lack skin repair benefits; others may have only one function and contain numerous active ingredients that can interact, making it difficult to control production scale and quality; still others may have overly complex preparation processes requiring a large amount of raw materials, making it difficult to guarantee production quality. Therefore, there is an urgent need for products with a simple and clearly defined composition of active ingredients, offering both excellent moisturizing and repairing functions. Summary of the Invention

[0003] Therefore, it is necessary to provide a composition, inclusion liposome, preparation method thereof, composite composition and application that have both moisturizing and repairing effects and a simple and clear composition of effective ingredients.

[0004] A first aspect of the present invention provides a composition having both moisturizing and repairing effects, comprising acetylglucosamine, adenosine, and sialic acid.

[0005] In some embodiments, the mass ratio of the acetylglucosamine, the adenosine, and the sialic acid is (0.1-3):(0.01-5):(0.1-1); optionally it is (0.6-1.2):(0.4-1.2):(0.2-0.8).

[0006] A second aspect of the present invention provides an inclusion liposome with dual moisturizing and repairing effects, comprising a membrane material and a composition with dual moisturizing and repairing effects according to the first aspect of the present invention, the composition being encapsulated within a capsule formed of the membrane material.

[0007] In some embodiments, the inclusion complex liposomes have at least one of the following characteristics:

[0008] (1) The membrane material includes one or more of lecithin and cholesterol;

[0009] Optionally, the membrane material comprises lecithin and cholesterol in a mass ratio of (2.5-7.5):1;

[0010] (2) The ratio of the total mass of the composition to the mass of the membrane material is 1:(1.3-2.0);

[0011] (3) The particle size of the inclusion complex liposomes is 100nm-200nm.

[0012] A third aspect of the present invention provides a method for preparing inclusion complex liposomes with dual moisturizing and repairing effects as described in the second aspect of the present invention, comprising the following steps:

[0013] Prepare an oil-phase organic solution containing the membrane material;

[0014] Prepare an aqueous active ingredient solution containing the acetyl glucosamine, the adenosine, and the sialic acid;

[0015] The inclusion complex liposomes are prepared by mixing the oil phase membrane material organic solution and the aqueous phase active ingredient solution, removing the organic solvent.

[0016] A fourth aspect of the present invention provides a composite composition having dual moisturizing and repairing effects, comprising the inclusion liposomes having dual moisturizing and repairing effects of the second aspect of the present invention or the inclusion liposomes having dual moisturizing and repairing effects prepared by the method of the third aspect of the present invention.

[0017] In some embodiments, the composite composition further includes one or more of a thickener, preservative, chelating agent, filler, emollient, emulsifier, and fragrance.

[0018] In some embodiments, the composite composition has at least one of the following characteristics:

[0019] (1) The thickener includes one or more of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, hydroxyethyl cellulose, carbomer, gum arabic, polymer EMT-10 and agarose;

[0020] Optionally, the thickener includes sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, and hydroxyethyl cellulose;

[0021] Further optionally, the mass ratio of the sodium acrylate / sodium acryloyl dimethyl taurate copolymer, the xanthan gum, and the hydroxyethyl cellulose in the thickener is (1.5-3.5):(3.5-4.7):(0.3-0.4);

[0022] (2) The preservatives include one or more of phenoxyethanol, pentylene glycol, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea and DMDM ​​hydantoin;

[0023] Optionally, the preservative includes phenoxyethanol and pentylene glycol;

[0024] Further optionally, the mass ratio of phenoxyethanol to pentanediol in the preservative is (0.3-0.5):(0.3-0.5);

[0025] (3) The chelating agent includes EDTA-2NA;

[0026] (4) The filler includes one or more of kaolin, titanium dioxide, cassava starch, sodium chloride, silica and magnesium sulfate;

[0027] Optionally, the filler includes kaolin and titanium dioxide;

[0028] Further optionally, the mass ratio of the kaolin to the titanium dioxide in the filler is (1.2-3.2):(1-3);

[0029] (5) The emollients include one or more of the following: phytosterols, squalane, tocopheryl acetate, behenol, isononyl isononanoate, glycerin, panthenol, isohexadecane, mannitol, sodium hyaluronate, propylene glycol, and wheat germ oil;

[0030] Optionally, the emollient includes phytosterols, squalane, tocopheryl acetate, behenol, and isononyl isononanoate;

[0031] Further optionally, the mass ratio of the phytosterols to the squalane in the emollient is (2.1-3.9):(1.5-4);

[0032] Further optionally, the mass ratio of the phytosterols and tocopheryl acetate in the emollient is (2.1-3.9):(2.3-4.1);

[0033] Further optionally, the mass ratio of the phytosterols and behenols in the emollient is (2.1-3.9):(1.5-3.7);

[0034] Further optionally, the mass ratio of the phytosterol to the isononyl isononanoate in the emollient is (2.1-3.9):(0.6-5.5);

[0035] (6) The emulsifier includes one or more of PEG-100 stearate, PEG-400 dilaurate, PEG-400 monolaurate, PEG-12 lauryl ether, PEG-20 sorbitan monostearate and cetearyl glucoside;

[0036] Optionally, the emulsifier includes PEG-100 stearate;

[0037] (7) The fragrances include daily-use fragrances.

[0038] In some embodiments, the composite composition comprises, by weight percentage, the following components:

[0039] 0.1%-3% acetylglucosamine, 0.01%-5% adenosine, 0.1%-1% sialic acid, 1.0%-10.0% lecithin, 0.2%-2.0% cholesterol, 1.5%-3.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3.5%-4.7% xanthan gum, 0.3%-0.4% hydroxyethyl cellulose, 0.3%-0.5% phenoxyethanol, 0.3%-0.5% pentanediol, 0.0 5%-0.1% EDTA-2NA, 1.2%-3.2% kaolin, 1%-3% titanium dioxide, 2.1%-3.9% phytosterols, 1.5%-4% squalane, 2.3%-4.1% tocopheryl acetate, 1.5%-3.7% behenol, 0.6%-5.5% isononyl isononanoate, 1.3%-2.6% PEG-100 stearate, 0.04%-0.4% daily fragrance, balance water.

[0040] In some embodiments, the composite composition comprises, by weight percentage, the following components:

[0041] 0.6%-1.2% acetylglucosamine, 0.4%-1.2% adenosine, 0.2%-0.8% sialic acid, 1.0%-10.0% lecithin, 0.2%-2.0% cholesterol, 1.5%-3.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3.5%-4.7% xanthan gum, 0.3%-0.4% hydroxyethyl cellulose, 0.3%-0.5% phenoxyethanol, 0.3%-0.5% pentanediol, 0 0.05%-0.1% EDTA-2NA, 1.2%-3.2% kaolin, 1%-3% titanium dioxide, 2.1%-3.9% phytosterols, 1.5%-4% squalane, 2.3%-4.1% tocopheryl acetate, 1.5%-3.7% behenol, 0.6%-5.5% isononyl isononanoate, 1.3%-2.6% PEG-100 stearate, 0.04%-0.4% fragrance, balance being water.

[0042] The fifth aspect of the present invention provides the use of the composite composition of the fourth aspect of the present invention, which has both moisturizing and repairing effects, in the preparation of skin care products or cosmetics with both moisturizing and repairing effects.

[0043] The above-mentioned compositions, inclusion liposomes and their preparation methods, composite compositions and their applications with dual moisturizing and repairing effects include acetyl glucosamine, adenosine and sialic acid. Acetyl glucosamine, adenosine and sialic acid each have moisturizing and repairing effects, and the combination of the three can synergistically enhance the effect, resulting in significant moisturizing and repairing effects. Compared with the weak moisturizing and repairing effects when using any one or two of the above three substances, the composition that includes acetyl glucosamine, adenosine and sialic acid at the same time has a significant improvement in the effects of hydration, moisturizing and skin repair. Detailed Implementation

[0044] To facilitate understanding of the present invention, a more complete description will be given below with reference to relevant embodiments. Preferred embodiments of the present invention are shown below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that a thorough and complete understanding of the disclosure of the present invention will be achieved.

[0045] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0046] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0047] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0048] This document only specifically discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form an unspecified range; and any lower limit can be combined with other lower limits to form an unspecified range, just as any upper limit can be combined with any other upper limit to form an unspecified range. Furthermore, each individually disclosed point or single value can itself serve as a lower or upper limit and be combined with any other point or single value or with other lower or upper limits to form an unspecified range.

[0049] Unless otherwise specified, the temperature parameters in this invention can be either constant temperature processing or processing within a certain temperature range. The constant temperature processing allows temperature fluctuations within the precision range controlled by the instrument.

[0050] In the description of the invention, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0051] Unless otherwise specified, all embodiments and optional embodiments of the present invention can be combined with each other to form new technical solutions. Unless otherwise specified, all technical features and optional technical features of the present invention can be combined with each other to form new technical solutions.

[0052] Unless otherwise specified, all steps of the present invention may be performed sequentially or randomly, but are preferably performed sequentially.

[0053] A first aspect of the present invention provides a composition having both moisturizing and repairing effects, comprising acetylglucosamine, adenosine, and sialic acid.

[0054] Acetyl glucosamine, abbreviated as NAG, or N-acyl glucosamine, mostly occurs in nature as chitosan. It is a monosaccharide similar to glucose, widely found in animals, plants, and the human body, and contains a nitrogen atom in its structure. NAG possesses high stability, high safety, high water solubility, and high moisturizing properties, allowing for large-scale production with easy control over production scale and quality. NAG can quickly penetrate the stratum corneum and reach the deeper layers of the skin, promoting skin repair, wound healing, and reducing wound infection. Being natural, NAG gently promotes keratin metabolism, enhances skin hydration, and makes the skin more radiant. As a natural moisturizer, long-term use not only does not weaken the skin barrier but also strengthens it, making oily and sensitive skin smoother, more delicate, and healthier. NAG can also penetrate the skin, promoting hyaluronic acid production, improving skin appearance, promoting water-oil balance, reducing fine lines, and beautifying the skin. It is an effective ingredient with both moisturizing and repairing effects.

[0055] Adenosine can replenish skin energy, significantly improve skin elasticity, promote skin cell metabolism, prevent skin tissue sagging, restructure the skin, and restore skin elasticity by tightening and shrinking pores.

[0056] Sialic acid, also known as N-acetylneuraminic acid or sialic acid, is a naturally occurring carbohydrate widely distributed in human tissues. It exists in the form of cell membrane glycoproteins and glycolipids, and possesses various biological functions, such as: skin brightening, anti-aging, and antioxidant effects; promoting brain development; promoting gut health and nutrient absorption; improving memory and combating Alzheimer's disease; and regulating immunity and fighting viruses. The content of sialic acid increases in areas of inflammation, achieving skin repair effects. It is one of the effective ingredients with both moisturizing and repairing properties.

[0057] Understandably, the combination of acetylglucosamine, adenosine, and sialic acid can synergistically enhance their effects, resulting in significant moisturizing and repairing benefits. Compared to the weak moisturizing and repairing effects when using any one or two of the above three substances, the combination of acetylglucosamine, adenosine, and sialic acid has a significantly improved effect in hydrating, moisturizing, and repairing the skin.

[0058] It should be noted that the hyaluronic acid mentioned above is a moisturizer that can form a protective film on the skin's surface, helping the skin retain moisture and improving dryness and roughness. It also promotes collagen production, contributing to anti-aging. The main functions and effects of hyaluronic acid are as follows: 1. Moisturizing: Hyaluronic acid has strong water-retention properties, absorbing surrounding moisture to form a protective film and prevent moisture loss, thus achieving a moisturizing effect; 2. Nourishing: Hyaluronic acid can penetrate the skin's surface, replenishing moisture and making the skin softer and smoother, thereby improving dryness and roughness; 3. Repairing: Hyaluronic acid can promote skin cell metabolism, accelerate the repair of damaged skin tissue, reduce skin damage, and maintain healthy skin; 4. Anti-aging: Hyaluronic acid can stimulate collagen synthesis in the skin, enhancing skin elasticity and reducing the appearance of wrinkles and fine lines, thus having a certain anti-aging effect.

[0059] In some embodiments, the mass ratio of acetylglucosamine, adenosine, and sialic acid is (0.1-3):(0.01-5):(0.1-1); for example, but not limited to (0.1-2.8):(0.1-4.8):(0.15-1), (0.2-2.6):(0.2-4.5):(0.2-1), (0.3-2.4):(0.3-4.2):(0.25-1), (0.4-2.2):(0.4-4):(0.3-1), (0.5-2):(0.5-3.7):(0.35-1), (0.6-1.8):(0.6-3.5):(0.4-1). The following are some examples of acetylglucosamine, adenosine, and sialic acid ratios: (0.7-1.6):(0.7-3.3):(0.45-1), (0.8-1.5):(0.8-2.8):(0.5-1), (0.9-1.4):(0.9-2.5):(0.55-1), (1-1.3):(1-2.3):(0.6-1), (1.1-1.3):(1.1-2):(0.65-1), (1-1.5):(1.1-1.7):(0.7-1), (0.9-1.5):(1.1-1.5):(0.7-0.9), or (1-1.4):(1.1-1.3):(0.75-0.85). When the mass ratio of acetylglucosamine, adenosine, and sialic acid is within the above range, it is more conducive to the synergistic moisturizing and repairing effects of the three. Optionally, the mass ratio of acetylglucosamine, adenosine, and sialic acid is (0.6-1.2):(0.4-1.2):(0.2-0.8).

[0060] A second aspect of the present invention provides an inclusion liposome with dual moisturizing and repairing effects, comprising a membrane material and a composition with dual moisturizing and repairing effects as described in the first aspect of the present invention, wherein the composition is encapsulated within a capsule formed by the membrane material. The liposome encapsulation technology encapsulates functional substances such as acetylglucosamine, adenosine, and sialic acid. This method protects the active functional substances, allowing them to more easily penetrate the skin barrier and reach the skin to exert their effects. This further facilitates the synergistic moisturizing and skin-repairing effects of the functional substances such as acetylglucosamine, adenosine, and sialic acid in the composition.

[0061] In some embodiments, the membrane material includes one or more of lecithin and cholesterol.

[0062] In some alternative embodiments, the membrane material comprises lecithin and cholesterol in a mass ratio of (2.5-7.5):1. For example, but not limited to, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1, 5.5:1, 6:1, 6.5:1, 7:1, 7.5:1, or any range between two of the above ratios. Optionally, the membrane material comprises lecithin and cholesterol in a mass ratio of (2.5-3.75):1.

[0063] In some embodiments, the ratio of the total mass of the composition to the mass of the membrane material is 1:(1.3-2.0); for example, it can be, but is not limited to, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2.0, or any range between two of the above ratios. When the ratio of the total mass of the composition to the mass of the membrane material is within the above range, the resulting liposomes have a round and regular morphology and uniform size.

[0064] As one possible implementation, the particle size of the inclusion liposomes is 100nm-200nm. For example, it can be, but is not limited to, 100nm, 110nm, 120nm, 130nm, 140nm, 150nm, 160nm, 170nm, 180nm, 190nm, 200nm, or any range between two of the above particle sizes.

[0065] A third aspect of the present invention provides a method for preparing inclusion liposomes with dual moisturizing and repairing effects as described in the second aspect of the present invention, comprising the following steps: preparing an oil phase organic solution containing a membrane material; preparing an aqueous phase active ingredient solution containing acetyl glucosamine, adenosine, and sialic acid; mixing the oil phase organic solution and the aqueous phase active ingredient solution, removing the organic solvent, and preparing inclusion liposomes.

[0066] In some embodiments, the step of preparing an oil-phase organic solution of membrane material includes: dissolving the membrane material in an organic mixed solution of chloroform and diethyl ether, shaking to dissolve until the solution is transparent, thereby obtaining an oil-phase organic solution of membrane material.

[0067] In some embodiments, the step of preparing an aqueous active ingredient solution containing acetylglucosamine, adenosine, and sialic acid includes: dissolving acetylglucosamine, adenosine, and sialic acid in a phosphate buffer to prepare an aqueous active phosphate buffer.

[0068] In some embodiments, a method for preparing inclusion liposomes with dual moisturizing and repairing effects includes the following steps: dissolving a membrane material in an organic mixture of chloroform and diethyl ether, shaking until the solution becomes transparent, to obtain an oil-phase membrane material organic solution; dissolving acetyl glucosamine, adenosine, and sialic acid in a phosphate buffer solution to obtain an aqueous-phase active phosphate buffer solution; mixing the oil-phase membrane material organic solution and the aqueous-phase active phosphate buffer solution evenly to obtain a stable W / O emulsion; subjecting the W / O emulsion to vacuum evaporation to fully hydrate it, removing the organic solvent, until a semi-solid gel state is formed; slowly adding a small amount of phosphate buffer solution to the semi-solid gel state, and ultrasonically filtering to obtain inclusion liposomes with uniform particle size and dual moisturizing and repairing effects.

[0069] A fourth aspect of the present invention provides a composite composition having dual moisturizing and repairing effects, comprising the inclusion liposomes having dual moisturizing and repairing effects of the second aspect of the present invention or the inclusion liposomes having dual moisturizing and repairing effects prepared by the method of the third aspect of the present invention.

[0070] The composite composition provided by this invention has dual effects of moisturizing and repairing the skin. The composition has a simple range of components with clearly defined effects, simultaneously achieving multiple skin-nourishing and beautifying effects, including increasing skin hydration and improving skin health. Furthermore, the components of the composite composition are mild and non-irritating, the manufacturing process is simple, and it is easy to control product quality and production scale. The use of liposome encapsulation technology ensures its moisturizing and skin-repairing efficacy.

[0071] In some embodiments, the composite composition further includes one or more of a thickener, preservative, chelating agent, filler, emollient, emulsifier, and fragrance.

[0072] In some embodiments, the thickener includes one or more of sodium acrylate / sodium acryloyldimethyl taurate copolymer, xanthan gum, hydroxyethyl cellulose, carbomer, gum arabic, polymer EMT-10, and agarose. Optionally, the thickener includes sodium acrylate / sodium acryloyldimethyl taurate copolymer, xanthan gum, and hydroxyethyl cellulose.

[0073] In some alternative embodiments, the mass ratio of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, and hydroxyethyl cellulose in the thickener is (1.5-3.5):(3.5-4.7):(0.3-0.4); for example, but not limited to (1.5-3.3):(3.6-4.7):(0.3-0.39), (1.5-3):(3.8-4.7):(0.3-0.38), (1.5-2.8):(4-4.7):(0.3-0.37), (1.5-2.5):(4.2-4.7):(0.3-0.36), (1.5-2.3):(4.4-4.7):(0.3-0.35), or (1.5-2):(4.5-4.7):(0.3-0.34), etc.

[0074] In some embodiments, the preservative includes one or more of phenoxyethanol, pentylene glycol, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea, and DMDM ​​hydantoin.

[0075] In some alternative embodiments, the preservative includes phenoxyethanol and pentylene glycol. Optionally, the mass ratio of phenoxyethanol to pentylene glycol in the preservative is (0.3-0.5):(0.3-0.5); for example, but not limited to (0.3-0.47):(0.33-0.5), (0.3-0.45):(0.35-0.5), (0.3-0.43):(0.38-0.5), (0.3-0.4):(0.4-0.5), (0.3-0.38):(0.43-0.5), (0.3-0.35):(0.45-0.5), or (0.3-0.33):(0.47-0.5), etc.

[0076] In some implementations, the chelating agent includes EDTA-2NA.

[0077] In some embodiments, the filler includes one or more of kaolin, titanium dioxide, cassava starch, sodium chloride, silica, and magnesium sulfate.

[0078] In some alternative embodiments, the filler includes kaolin and titanium dioxide. Optionally, the mass ratio of kaolin to titanium dioxide in the filler is (1.2-3.2):(1-3); for example, but not limited to (1.5-3.2):(1.2-3), (1.7-3.2):(1.5-3), (2-3.2):(1.8-3), (2.2-3.2):(2-3), (2.5-3.2):(2.3-3), (2.7-3.2):(2.5-3), or (3-3.2):(2.7-3), etc.

[0079] In some embodiments, the emollient includes one or more of phytosterols, squalane, tocopheryl acetate, behenol, isononyl isononanoate, glycerin, panthenol, isohexadecane, mannitol, sodium hyaluronate, propylene glycol, and wheat germ oil.

[0080] In some alternative embodiments, the emollients include phytosterols, squalane, tocopheryl acetate, behenol, and isononyl isononanoate.

[0081] Optionally, the mass ratio of phytosterols to squalane in the emollient is (2.1-3.9):(1.5-4); for example, it can be, but is not limited to, (2.3-3.9):(1.7-4), (2.5-3.9):(2-4), (2.7-3.9):(2.3-4), (3-3.9):(2.5-4), (3.3-3.9):(2.8-4), (3.4-3.8):(3-4), (3.4-3.7):(3.3-4), or (3.4-3.6):(3.5-4), etc.

[0082] Optionally, the mass ratio of phytosterols to tocopheryl acetate in the emollient is (2.1-3.9):(2.3-4.1); for example, it can be, but is not limited to, (2.3-3.9):(2.4-4), (2.5-3.9):(2.5-3.9), (2.7-3.9):(2.6-3.8), (3-3.9):(2.7-3.7), (3.3-3.9):(2.8-3.6), (3.4-3.8):(2.8-3.5), (3.4-3.7):(2.8-3.4), or (3.4-3.6):(2.8-3.3), etc.

[0083] Optionally, the mass ratio of phytosterols and behenols in the emollient is (2.1-3.9):(1.5-3.7); for example, but not limited to (2.3-3.9):(1.5-3.5), (2.5-3.9):(1.5-3.3), (2.7-3.9):(1.5-3), (3-3.9):(1.5-2.7), (3.3-3.9):(1.5-2.5), (3.4-3.8):(1.5-2.2), (3.4-3.7):(1.5-2), or (3.4-3.6):(1.5-1.7), etc.

[0084] Optionally, the mass ratio of phytosterols to isononyl isononanoate in the emollient is (2.1-3.9):(0.6-5.5); for example, it can be, but is not limited to, (2.3-3.9):(1-5.2), (2.5-3.9):(1.2-5), (2.7-3.9):(1.5-4.8), (3-3.9):(1.8-4.5), (3.3-3.9):(2-4.3), (3.4-3.8):(2.3-4), (3.4-3.7):(2.5-3.7), or (3.4-3.6):(2.7-3.5), etc.

[0085] In some embodiments, the emulsifier includes one or more of PEG-100 stearate, PEG-400 dilaurate, PEG-400 monolaurate, PEG-12 lauryl ether, PEG-20 sorbitan monostearate, and cetearyl glucoside.

[0086] In some alternative embodiments, the emulsifier includes PEG-100 stearate.

[0087] In some implementations, the fragrance includes everyday fragrances.

[0088] In some embodiments, the composite composition comprises, by weight percentage, the following components:

[0089] 0.6%-1.2% acetylglucosamine, 0.4%-1.2% adenosine, 0.2%-0.8% sialic acid, 1.0%-10.0% lecithin, 0.2%-2.0% cholesterol, 1.5%-3.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3.5%-4.7% xanthan gum, 0.3%-0.4% hydroxyethyl cellulose, 0.3%-0.5% phenoxyethanol, 0.3%-0.5% pentanediol, 0 0.05%-0.1% EDTA-2NA, 1.2%-3.2% kaolin, 1%-3% titanium dioxide, 2.1%-3.9% phytosterols, 1.5%-4% squalane, 2.3%-4.1% tocopheryl acetate, 1.5%-3.7% behenol, 0.6%-5.5% isononyl isononanoate, 1.3%-2.6% PEG-100 stearate, 0.04%-0.4% fragrance, balance being water.

[0090] The mass percentage content of acetylglucosamine in the composite composition is 0.6%-1.2%; for example, it can be, but is not limited to, one or more of 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, or 1.2%. Optionally, the mass percentage content of acetylglucosamine in the composite composition is 0.8%-1.2%.

[0091] The adenosine content in the composite composition is 0.4%-1.2% by mass; for example, it can be, but is not limited to, one or more of 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, or 1.2%. Optionally, the adenosine content in the composite composition is 0.8%-1.2% by mass.

[0092] The mass percentage content of sialic acid in the composite composition is 0.2%-0.8%; for example, it can be, but is not limited to, one or more of 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, or 0.8%. Optionally, the mass percentage content of sialic acid in the composite composition is 0.4%-0.8%.

[0093] The lecithin content in the composite composition is 1.0%-10.0% by mass; for example, it can be, but is not limited to, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, or any range between two of the above values. Optionally, the lecithin content in the complex is 2.5%-3.75% by mass.

[0094] The cholesterol content in the composite composition is 0.2%-2.0% by mass; for example, it can be, but is not limited to, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, or any two of the above values.

[0095] The mass percentage content of sodium acrylate / sodium acryloyldimethyl taurate copolymer in the composite composition is 1.5%-3.5%; for example, it can be, but is not limited to, one or more of 1.5%, 1.7%, 2%, 2.3%, 2.5%, 2.7%, 3%, 3.3% or 3.5%.

[0096] The xanthan gum content in the composite composition is 3.5%-4.7% by mass; for example, it can be, but is not limited to, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7% or any range between two of the above values.

[0097] The mass percentage content of hydroxyethyl cellulose in the composite composition is 0.3%-0.4%; for example, it can be, but is not limited to, 0.3%, 0.31%, 0.32%, 0.33%, 0.34%, 0.35%, 0.36%, 0.37%, 0.38%, 0.39%, 0.4% or any two of the above values.

[0098] The mass percentage content of phenoxyethanol in the composite composition is 0.3%-0.5%; for example, it can be, but is not limited to, 0.3%, 0.32%, 0.35%, 0.37%, 0.4%, 0.42%, 0.45%, 0.48%, 0.5%, or any range between two of the above values.

[0099] The mass percentage content of pentanediol in the composite composition is 0.3%-0.5%; for example, it can be, but is not limited to, 0.3%, 0.32%, 0.35%, 0.37%, 0.4%, 0.42%, 0.45%, 0.48%, 0.5%, or any range between any two of the above values.

[0100] The mass percentage content of EDTA-2NA in the composite composition is 0.05%-0.1%; for example, it can be, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1% or any two of the above values.

[0101] The mass percentage content of kaolin in the composite composition is 1.2%-3.2%; for example, it can be, but is not limited to, 1.2%, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.2% or any two of the above values.

[0102] The mass percentage content of titanium dioxide in the composite composition is 1%-3%; for example, it can be, but is not limited to, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.8%, 3% or any two of the above values.

[0103] The mass percentage content of phytosterols in the composite composition is 2.1%-3.9%; for example, it can be, but is not limited to, 2.1%, 2.3%, 2.5%, 2.8%, 3%, 3.3%, 3.5%, 3.7%, 3.9% or any two of the above values.

[0104] The mass percentage content of squalane in the composite composition is 1.5%-4%; for example, it can be, but is not limited to, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.3%, 3.5%, 3.7%, 4% or any two of the above values.

[0105] The mass percentage content of tocopherol acetate in the composite composition is 2.3%-4.1%; for example, it can be, but is not limited to, 2.3%, 2.5%, 2.8%, 3%, 3.3%, 3.5%, 3.7%, 3.9%, 4.1% or any two of the above values.

[0106] The mass percentage content of behenol in the composite composition is 1.5%-3.7%; for example, it can be, but is not limited to, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.3%, 3.5%, 3.7% or any two of the above values.

[0107] The mass percentage content of isononyl isononanoate in the composite composition is 0.6%-5.5%; for example, it can be, but is not limited to, 0.6%, 0.8%, 1%, 1.3%, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.8%, 3%, 3.3%, 3.5%, 3.7%, 4%, 4.3%, 4.5%, 4.8%, 5%, 5.3%, 5.5%, or any range between two of the above values.

[0108] The mass percentage content of PEG-100 stearate in the composite composition is 1.3%-2.6%; for example, it can be, but is not limited to, 1.3%, 1.5%, 1.8%, 2%, 2.3%, 2.5%, 2.6% or any two of the above values.

[0109] The mass percentage content of daily fragrance in the composite composition is 0.04%-0.4%; for example, it can be, but is not limited to, 0.04%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4% or any two of the above values.

[0110] The fifth aspect of the present invention provides the application of the composite composition of the fourth aspect, which has both moisturizing and repairing effects, in the preparation of skin care products or cosmetics with both moisturizing and repairing effects.

[0111] The technical solution of the present invention will be described below through specific embodiments.

[0112] I. Formulation of a compound composition with dual moisturizing and repairing effects

[0113] The formulations of each embodiment and comparative example are shown in Table 1.

[0114] Table 1

[0115]

[0116]

[0117] II. Preparation process of composite combination

[0118] The preparation methods for Examples 1-3 are as follows:

[0119] Step 1: Dissolve lecithin and cholesterol in a chloroform-ether organic mixture and shake until the solution becomes transparent to prepare an oil phase membrane material organic solution; dissolve acetyl glucosamine, adenosine, and sialic acid in phosphate buffer to prepare an aqueous phase active phosphate buffer; mix the oil phase membrane material organic solution and the aqueous phase active phosphate buffer evenly to obtain a stable W / O type emulsion; perform vacuum evaporation on the W / O type emulsion to fully hydrate it and remove the organic solvent until a semi-solid gel is formed; slowly add a small amount of phosphate buffer to the semi-solid gel and ultrasonically filter to obtain inclusion liposomes with uniform particle size and dual moisturizing and repairing effects.

[0120] Step 2: Wet and disperse the thickener, preservative and chelating agent completely in deionized water to obtain mixture 1.

[0121] Step 3: Add filler, emollient and inclusion liposome to the mixture, heat to 60℃, and homogenize at 3000r / min for 3-5 minutes to ensure complete dispersion, to obtain mixture 2.

[0122] Step 4: Add emulsifier and fragrance to the mixture 2 obtained in step 3, homogenize at 3000 r / min for 3 min to ensure complete emulsification, and heat to 60℃ to obtain a face cream composition that improves skin radiance.

[0123] Comparative Examples 1-7

[0124] The difference between the preparation methods of the composite compositions in Comparative Examples 1-7 and the preparation method of the composite composition in Example 3 is that the functional active substances added during the preparation of the inclusion complex liposomes are different, while all other aspects are the same.

[0125] III. Performance Testing

[0126] 1. Safety test: Each product prepared in Table 1 was placed in a patch applicator. The patch applicator containing the test substance was applied to the flexor side of the forearm of the test subjects (33 subjects). The patch applicator was gently pressed with the palm of the hand to make it evenly applied to the skin for 24 hours. Skin irritation and sensitization were observed according to the (Skin reaction grading standard for closed patch test) at 30 minutes, 24 hours and 48 hours after the patch applicator was removed, and the observation results were recorded.

[0127] The skin reaction grading criteria are as follows:

[0128] Grade 0: Negative reaction;

[0129] Grade 1: Suspicious reaction; only slight erythema;

[0130] Grade 2: Weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0131] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, vesicles; the reaction may extend beyond the tested area;

[0132] Grade 4: Extremely strong reaction (confluent herpes simplex reaction); obvious erythema, severe infiltration, edema, confluent herpes simplex, reaction extending beyond the test area.

[0133] The results showed that the subjects using the test products of Examples 1-3 and Comparative Examples 1-7 all scored 0 at 30 min, 24 hours and 48 hours, and no adverse skin reactions were observed. This indicates that the composite composition of the present invention, which has both moisturizing and repairing effects, is safe and non-irritating to the skin.

[0134] 2. Human efficacy testing

[0135] (1) Subjects: 100 healthy volunteers, all female, aged 20-55 years, were divided into 10 groups of 10 subjects each. Each group used the test products of Examples 1-3 and Comparative Examples 1-7 respectively.

[0136] All enrolled participants were healthy volunteers from Shanghai, with no history of skin diseases or other serious illnesses within the past three months, and who had not taken any antibiotics or hormonal medications. Written informed consent forms were provided to all participants before the study began, and they signed the forms after thorough communication. This study was conducted under the strict supervision and guidance of the ethics committee, and the personal privacy and data security of the participants were rigorously guaranteed.

[0137] Environmental conditions: Set the experimental environment conditions according to the experimental requirements (temperature 20-22℃, humidity 40-60%), and the subjects should adapt to the experimental environment for more than 15-30 minutes.

[0138] (2) Test instruments

[0139] 1. TEWL ( Combo)

[0140] 2. Cornermeter Combo)

[0141] Table 2

[0142]

[0143]

[0144] (3) Experimental Methods: A series of tests were conducted on volunteers using various instruments. First, the subjects who passed the selection / exclusion screening form arrived at the testing center to sign the informed consent form, wash their face, straighten their hair, and sit quietly for 30 minutes. During the sitting period, the selection / exclusion screening form and skin type form were used. The stratum corneum moisture content and skin TEWL value of each subject's skin were tested before use and after 2, 4, and 8 weeks of use. After obtaining the corresponding data, the change rate based on the pre-use data was obtained. The subjects evaluated their satisfaction with the tested product based on relevant indicators after 2, 4, and 8 weeks of use.

[0145] (4) Data analysis: After calculating the average value of the data obtained from each experiment, the average value of the relevant parameters was obtained by comparing the data of different embodiments or proportions with the average value measured before the trial.

[0146] (5) Instrument data processing and analysis

[0147] 1) Analysis of changes in the moisture content of the stratum corneum of the subjects' skin over time

[0148] After digital processing using the Cornermeter instrument, the skin stratum corneum moisture content values ​​for each subject across various embodiments and comparative examples can be obtained. The mean skin stratum corneum moisture content data of the 10 subjects in each group measured before using the test sample is BL1.

[0149] Rate of change at different times after use of the test product = (mean stratum corneum moisture content of 10 subjects in each group after use of the test product - BL1) / BL1

[0150] Table 3 shows the rate of change in the mean water content of the stratum corneum of the skin.

[0151] Table 3

[0152]

[0153] As shown in Table 3, the combination of acetylglucosamine, adenosine, and sialic acid effectively increases the moisture content of the stratum corneum, thus providing a moisturizing effect. Furthermore, in Example 3, the subjects exhibited a generally higher stratum corneum moisture content compared to the other groups, indicating the optimal moisturizing effect.

[0154] 2) Analysis of the changes in transepidermal water loss (TEWL) values ​​of subjects over time.

[0155] The mean TEWL (transepidermal water loss) value of each subject across all embodiments and comparative examples can be obtained using the instrument TEWL. The mean TEWL value of the skin transepidermal water loss of 10 subjects in each group, measured before the use of the test sample, is BL2.

[0156] The rate of change at different times after using the test product = (mean transepidermal water loss (TEWL) of 10 subjects in each group after using the test product - BL2) / BL2

[0157] Table 4 shows the rate of change of the mean transepidermal water loss (TEWL) value of the skin.

[0158] Table 4

[0159]

[0160] Results from four studies indicate that the combination of acetylglucosamine, adenosine, and sialic acid effectively reduces the transepidermal water loss (TEWL) value of the skin, demonstrating a repairing effect. Furthermore, in Example 3, the subjects showed a generally lower TEWL value compared to the other groups, indicating the best repair effect.

[0161] It should be noted that transdermal water loss (TEWL), also known as transdermal water loss, represents the loss of water from the deep dermis through evaporation from the epidermis. It is an important parameter describing the skin barrier and is closely related to the water content of the stratum corneum. The higher the TEWL value, the more water is lost through the skin, and the poorer the barrier function of the stratum corneum.

[0162] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0163] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. An inclusion liposome with dual moisturizing and repairing effects, characterized in that, The invention includes a membrane material and a composition having both moisturizing and repairing effects, the composition being encapsulated within a capsule formed by the membrane material; the composition consists of acetyl glucosamine, adenosine, and sialic acid, wherein the mass ratio of acetyl glucosamine, adenosine, and sialic acid is (0.1-3):(0.01-5):(0.1-1); the membrane material includes lecithin and cholesterol in a mass ratio of (2.5-7.5):

1.

2. The inclusion complex liposome according to claim 1, characterized in that, The mass ratio of the acetyl glucosamine, the adenosine, and the sialic acid is (0.6-1.2):(0.4-1.2):(0.2-0.8).

3. The inclusion complex liposome according to claim 1, characterized in that, The inclusion complex liposome has at least one of the following characteristics: (1) The ratio of the total mass of the composition to the mass of the membrane material is 1:(1.3-2.0); (2) The particle size of the inclusion complex liposomes is 100nm-200nm.

4. A method for preparing an inclusion complex liposome with dual moisturizing and repairing effects as described in any one of claims 1 to 3, characterized in that, Includes the following steps: Prepare an oil-phase organic solution containing the membrane material; Prepare an aqueous active ingredient solution containing the acetyl glucosamine, the adenosine, and the sialic acid; The inclusion complex liposomes are prepared by mixing the oil phase membrane material organic solution and the aqueous phase active ingredient solution, removing the organic solvent.

5. A composite composition with dual moisturizing and repairing effects, characterized in that, Includes inclusion liposomes with dual moisturizing and repairing effects as described in any one of claims 1 to 3, or inclusion liposomes with dual moisturizing and repairing effects prepared by the method described in claim 4.

6. The composite composition according to claim 5, characterized in that, The composite composition also includes one or more of the following: thickener, preservative, chelating agent, filler, emollient, emulsifier, and fragrance.

7. The composite composition according to claim 6, characterized in that, The composite composition has at least one of the following characteristics: (1) The thickener includes one or more of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum, hydroxyethyl cellulose, carbomer, gum arabic, polymer EMT-10 and agarose; (2) The preservatives include one or more of phenoxyethanol, pentylene glycol, phenoxyethanol, methylparaben, propylparaben, imidazolidinyl urea and DMDM ​​hydantoin; (3) The chelating agent includes EDTA-2NA; (4) The filler includes one or more of kaolin, titanium dioxide, cassava starch, sodium chloride, silica and magnesium sulfate; (5) The emollients include one or more of the following: phytosterols, squalane, tocopheryl acetate, behenol, isononyl isononanoate, glycerin, panthenol, isohexadecane, mannitol, sodium hyaluronate, propylene glycol, and wheat germ oil; (6) The emulsifier includes one or more of PEG-100 stearate, PEG-400 dilaurate, PEG-400 monolaurate, PEG-12 lauryl ether, PEG-20 sorbitan monostearate and cetearyl glucoside; (7) The fragrances include daily-use fragrances.

8. The composite composition according to claim 7, characterized in that, The composite composition has at least one of the following characteristics: (1) The thickener includes sodium acrylate / sodium acryloyl dimethyl taurate copolymer, xanthan gum and hydroxyethyl cellulose; (2) The mass ratio of the sodium acrylate / sodium acryloyl dimethyl taurate copolymer, the xanthan gum, and the hydroxyethyl cellulose in the thickener is (1.5-3.5):(3.5-4.7):(0.3-0.4); (3) The preservatives include phenoxyethanol and pentanediol; (4) The mass ratio of phenoxyethanol to pentanediol in the preservative is (0.3-0.5):(0.3-0.5); (5) The filler includes kaolin and titanium dioxide; (6) The mass ratio of the kaolin and the titanium dioxide in the filler is (1.2-3.2):(1-3); (7) The moisturizing lotion includes phytosterols, squalane, tocopheryl acetate, behenol and isononyl isononanoate; (8) The mass ratio of the phytosterols and squalane in the emollient is (2.1-3.9):(1.5-4); (9) The mass ratio of the phytosterols and tocopheryl acetate in the emollient is (2.1-3.9):(2.3-4.1); (10) The mass ratio of the phytosterols and behenols in the emollient is (2.1-3.9):(1.5-3.7); (11) The mass ratio of the phytosterol to the isononyl isononanoate in the emollient is (2.1-3.9):(0.6-5.5); (12) The emulsifier includes PEG-100 stearate.

9. The composite composition according to any one of claims 5 to 8, characterized in that, The composite composition comprises the following components by weight percentage: 0.6%-1.2% acetylglucosamine, 0.4%-1.2% adenosine, 0.2%-0.8% sialic acid, 1.0%-10.0% lecithin, 0.2%-2.0% cholesterol, 1.5%-3.5% sodium acrylate / sodium acryloyldimethyl taurate copolymer, 3.5%-4.7% xanthan gum, 0.3%-0.4% hydroxyethyl cellulose, 0.3%-0.5% phenoxyethanol, 0.3%-0.5% pentanediol, 0 0.05%-0.1% EDTA-2NA, 1.2%-3.2% kaolin, 1%-3% titanium dioxide, 2.1%-3.9% phytosterols, 1.5%-4% squalane, 2.3%-4.1% tocopheryl acetate, 1.5%-3.7% behenol, 0.6%-5.5% isononyl isononanoate, 1.3%-2.6% PEG-100 stearate, 0.04%-0.4% fragrance, balance being water.

10. The use of the composite composition having dual moisturizing and repairing effects as described in any one of claims 5 to 9 in the preparation of skin care products having dual moisturizing and repairing effects.

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