High-efficiency phosphorus-solubilizing bio-organic fertilizer

Abstract

The application discloses a high-efficiency phosphorus-dissolving bio-organic fertilizer, which comprises phosphorus-dissolving bacteria and an organic matrix, and is characterized in that the mass ratio of Aspergillus niger and Rhizobium rhizagapanthus is 1:10 to 10:1; the Aspergillus niger is Aspergillus niger A44 with a preservation number of CGMCC No.11793; and the Rhizobium rhizagapanthus is Rhizobium rhizagapanthus 3T7 with a preservation number of CGMCC No.21507. The phosphorus-dissolving performance of the Aspergillus niger and the Rhizobium rhizagapanthus is synergistically improved, so that the availability of phosphorus in soil is improved, and the growth of plants is promoted.

Description

Technical Field

[0001] This invention relates to a highly efficient phosphorus-soluble bio-organic fertilizer. Background Technology

[0002] A widespread problem in current agricultural production is insufficient soil phosphorus content and low efficiency in phosphate fertilizer utilization. Phosphorus is a crucial nutrient element indispensable for plant growth, development, and yield formation, second only to nitrogen. However, the availability of phosphorus in farmland soils is generally low, leading to limited plant growth and thus affecting agricultural yield. This problem stems from multiple factors, including soil phosphorus fixation, differences in soil type, unreasonable fertilization methods, and a lack of efficient phosphorus-solubilizing microorganisms.

[0003] First, soil phosphorus fixation is the main reason for the reduced availability of phosphorus. Iron, aluminum, and calcium ions in the soil combine with phosphorus to form insoluble phosphorus compounds, making it difficult for plants to absorb the phosphorus. Even with large applications of phosphate fertilizers, the absorption and utilization rate of phosphorus by plants remains low, resulting in poor phosphate fertilizer utilization efficiency.

[0004] Secondly, my country has diverse soil types, each with varying capacities for phosphorus absorption and fixation. For example, calcareous soils and yellow soils have a stronger capacity for phosphorus fixation, further reducing soil phosphorus availability. This necessitates developing appropriate phosphate fertilizer application strategies tailored to different soil types to improve phosphate fertilizer utilization.

[0005] Furthermore, insufficient and improper fertilization has long been a problem in agricultural production. Many farmers rely excessively on chemical fertilizers, neglecting the importance of organic and microbial fertilizers. This leads to reduced soil biological activity and affects the effectiveness of phosphate fertilizers. Therefore, the rational combination of organic, chemical, and microbial fertilizers is essential to improve soil biological activity, thereby enhancing phosphorus release and plant absorption.

[0006] It is worth mentioning that Chinese patent application number 2015109209178 has disclosed a highly efficient phosphorus-solubilizing bacteria, a bio-organic fertilizer prepared from it, and its application. This invention can dissolve phosphate rock powder, iron phosphate, aluminum phosphate, zinc phosphate, and tricalcium phosphate to a certain extent, but its phosphorus-solubilizing effect still needs to be improved. Summary of the Invention

[0007] In view of the shortcomings of the existing technology, the technical problem to be solved by the present invention is to provide a highly efficient phosphorus-soluble bio-organic fertilizer with good phosphorus-soluble ability.

[0008] A highly efficient phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate, wherein the phosphorus-solubilizing bacteria are Aspergillus niger and Rhizobium agapanthus.

[0009] Preferably, the Aspergillus niger is Aspergillus niger A44, accession number: CGMCCNo.11793.

[0010] Among them, Aspergillus niger A44 is highly efficient at dissolving phosphorus, which is achieved through the following mechanism:

[0011] Production of acidic substances: During its growth, *Aspergillus niger* A44 produces acidic substances, such as organic acids and acidic metabolites. These acidic substances lower the pH of the soil surrounding the fungus, causing insoluble phosphorus to gradually transform into a more soluble and absorbable form. Organic acids, such as citric acid, succinic acid, and acetic acid, can form soluble complexes with insoluble phosphorus in the soil, thereby increasing the solubility of phosphorus in the soil.

[0012] Production of acid phosphatase: The acid phosphatase produced by Aspergillus niger A44 can catalyze the hydrolysis of organic phosphorus, converting it into inorganic phosphorus that can be absorbed by plants. This enzyme plays an important role in the mineralization process of organic phosphorus and helps to improve the utilization rate of soil phosphorus.

[0013] Mycelial Adsorption: The mycelia of Aspergillus niger A44 have an adsorption capacity, capable of adsorbing phosphate ions in the soil and preventing them from forming insoluble phosphorus with calcium, iron, aluminum, and other minerals in the soil. Phosphate ions adsorbed on the surface of the mycelia are more easily absorbed by plant roots, thereby increasing the availability of phosphorus in the soil.

[0014] Plant root induction: Aspergillus niger A44 forms a symbiotic relationship with plant roots, stimulating root growth by secreting compounds and acidic substances. This encourages vigorous root growth near the mycelium, promoting phosphorus absorption and further enhancing phosphorus utilization.

[0015] In summary, Aspergillus niger A44 enhances the availability of phosphorus in the soil and improves the utilization rate of phosphate fertilizer by producing acidic substances, acid phosphatase, mycelial adsorption, and symbiosis with plant roots.

[0016] Preferably, the rhizobium of Agapanthus is Rhizobium rhizagapanthus 3T7, accession number: CGMCC No. 21507.

[0017] Rhizobium in the rhizosphere of Agapanthus can efficiently solubilize phosphorus through the following mechanism:

[0018] Acidification: Rhizobium in the rhizosphere of Agapanthus may acidify the soil environment by secreting organic acids, thereby dissolving inorganic phosphorus compounds in the soil. The acidic effect of organic acids can cause aluminum and iron ions in the soil to dissociate and form soluble aluminum phosphate or iron phosphate compounds with phosphorus compounds, thus increasing the availability of phosphorus in the soil.

[0019] Enzymatic hydrolysis: Rhizobium in the rhizosphere of Agapanthus may secrete enzymes such as phosphatases or acid phosphatases, which can directly decompose inorganic phosphates into phosphate ions, thereby increasing the availability of phosphorus in the soil.

[0020] Adsorption by bacteria: The surface of Rhizobium agapanis in the rhizosphere may have some phosphorus-loving functional groups, which can adsorb phosphate and convert it into a usable form of phosphorus, thereby improving the plant's efficiency in absorbing and utilizing phosphorus.

[0021] In summary, the rhizobia of Agapanthus can promote the decomposition and conversion of inorganic phosphorus compounds into usable phosphorus forms by secreting organic acids, enzymes, or functional groups on the surface of the bacteria, thereby increasing the availability of phosphorus in the soil and promoting plant growth.

[0022] Preferably, the phosphate-solubilizing bacteria are composed of Aspergillus niger and Rhizobium agapanthus in a mass ratio of 1:10 to 10:1.

[0023] The inventors discovered through research that *Aspergillus niger* and *Rhizobium agapanthus* rhizosphere nodules have a synergistic effect on phosphorus solubility when their mass ratio is 1:10 to 10:1. The inventors analyzed the possible mechanism as follows:

[0024] Metabolic product diversity: Aspergillus niger and Rhizobium agapanis have different metabolic pathways and metabolites, which can interact to enhance phosphate solubility and improve phosphorus availability in the soil. For example, Aspergillus niger can produce various organic acids (such as citric acid and malic acid), while Rhizobium agapanis can produce substances such as IAA, which can promote phosphate decomposition and improve phosphorus availability in the soil.

[0025] Altering the ecological environment: Aspergillus niger and Rhizobium agapanthus exist in different ecological environments. When they work together in the soil, they can alter the soil environment, thereby affecting phosphate transformation. For example, Aspergillus niger can lower the soil pH, making the soil environment more acidic, thus promoting the release of phosphate and its conversion into a usable form.

[0026] Variation in growth conditions: Aspergillus niger and Rhizobium agapanthus may produce different metabolites under different growth conditions, thus affecting phosphate solubility and improving the availability of phosphorus in the soil. For example, when Aspergillus niger and Rhizobium agapanthus work together, they may produce more enzymes such as phosphatases and nucleases, thereby promoting phosphate decomposition and improving the availability of phosphorus in the soil.

[0027] Promotional Interactions: When *Aspergillus niger* and *Rhizobium agapanthus* work together, they may exhibit synergistic effects, such as mutual stimulation and synergistic metabolism, which may lead to a synergistic enhancement of their phosphorus-solubilizing properties. For example, when *Aspergillus niger* and *Rhizobium agapanthus* work together, they may promote cell adsorption and phosphate decomposition, thereby increasing the availability of phosphorus in the soil. Furthermore, they may produce synergistic metabolites that may interact to enhance phosphate solubility and improve phosphorus availability in the soil. For instance, *Aspergillus niger* and *Rhizobium agapanthus* can produce biochelating agents and acidifying substances that can bind with inorganic phosphorus in the soil to form soluble organic phosphorus compounds, thereby increasing the availability of phosphorus in the soil.

[0028] In summary, the synergistic effect of *Aspergillus niger* and *Rhizobium agapanthus* rhizobia at a mass ratio of 1:10 to 10:1 may be due to multiple mechanisms of interaction, resulting in a synergistic effect. These mechanisms include the diversity of metabolites, changes in the ecological environment, variations in growth conditions, and the promotion of interactions. The interaction of these factors may lead to a synergistic enhancement of their phosphorus-solubilizing properties, thereby improving the availability of phosphorus in the soil and promoting plant growth.

[0029] In future research, the inventors will continue to determine its core mechanism.

[0030] Preferably, the number of live bacteria in the high-efficiency phosphorus-soluble bio-organic fertilizer is 0.1-0.4 billion / g.

[0031] Preferably, the organic matrix is ​​at least one of the following: livestock and poultry manure, decomposed straw, peat moss, rice husk powder, wheat husk powder, soybean meal powder, etc.

[0032] Preferably, the livestock and poultry manure is one or more mixtures of chicken manure, cow manure, sheep manure, horse manure, and pig manure.

[0033] Preferably, the high-efficiency phosphorus-soluble bio-organic fertilizer further includes one or more additives, binders, and protectants.

[0034] The binder is one or more mixtures of attapulgite powder and starch.

[0035] Preferably, a high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate, wherein the phosphorus-solubilizing bacteria are Aspergillus niger and Rhizobium agapanthus.

[0036] Preferably, the phosphate-solubilizing bacteria are composed of Aspergillus niger and Rhizobium agapanthus in a mass ratio of 1:10 to 10:1. Preferably, the phosphate-solubilizing bacteria are present in a concentration of 0.5-1 parts by weight.

[0037] Preferably, the organic matrix is ​​composed of the following raw materials in parts by weight: 5-20 parts by weight of livestock and poultry manure, 10-30 parts by weight of peat moss, 10-15 parts by weight of rice husk powder, and 5-10 parts by weight of soybean meal powder.

[0038] The highly efficient phosphorus-soluble bio-organic fertilizer is in powder or granular form.

[0039] Preferably, a granular, high-efficiency phosphorus-soluble bio-organic fertilizer further includes 1-8 parts by weight of a binder.

[0040] Preferably, the peat moss is 120 mesh.

[0041] Preferably, the particle size of the rice husk powder and soybean meal powder is 0.4-1 mm;

[0042] This application also discloses a method for preparing a highly efficient phosphorus-soluble bio-organic fertilizer.

[0043] The preparation method of the high-efficiency phosphorus-solubilizing bio-organic fertilizer includes the following steps: measuring the above-mentioned phosphorus-solubilizing bacteria and organic matrix according to the production ratio, mixing them evenly with a mixer, and preparing powdered high-efficiency phosphorus-solubilizing bio-organic fertilizer; or measuring the above-mentioned phosphorus-solubilizing bacteria, organic matrix, and binder according to the production ratio, mixing them evenly with a mixer, and preparing granular high-efficiency phosphorus-solubilizing bio-organic fertilizer.

[0044] Compared with the prior art, the present invention has the following beneficial technical effects:

[0045] (1) This invention uses livestock and poultry manure, peat soil, rice husk powder and soybean meal powder as raw materials, and Aspergillus niger and Rhizobium agapanthus as active biological bacteria to prepare high-efficiency phosphorus-soluble bio-organic fertilizer, which provides nutrients for crop growth, improves soil organic matter, soil aeration, increases microbial activity, thereby improving soil activity and effectively preventing soil compaction.

[0046] (2) This application introduces *Aspergillus niger* and *Rhizobium agapanthus* rhizosphere nodules. When *Aspergillus niger* and *Rhizobium agapanthus* rhizosphere nodules work together, they may produce synergistic effects, such as mutual stimulation and synergistic metabolism. These effects may lead to a synergistic enhancement of their phosphorus-solubilizing properties. They may promote cell adsorption and phosphate decomposition, thereby improving the availability of phosphorus in the soil. In addition, they may also produce some synergistic metabolites, which may interact with each other to enhance phosphate solubility and improve the availability of phosphorus in the soil. Detailed Implementation

[0047] (1) Strain name: Aspergillus niger A44

[0048] Latin name: Aspergillus niger

[0049] Preservation Institution: China General Microbiological Culture Collection Center, China Microbiological Culture Collection Committee

[0050] Collection institution abbreviation: CGMCC

[0051] Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing

[0052] Date of deposit: December 8, 2015

[0053] Registered with the China National Collection Center (CGMCC) No. 11793.

[0054] (2) Strain name: Rhizobium 3T7 of Agapanthus rhizosphere

[0055] Latin name: Rhi zobi um rhi zagapanthus

[0056] Preservation Institution: China General Microbiological Culture Collection Center, China Microbiological Culture Collection Committee

[0057] Collection institution abbreviation: CGMCC

[0058] Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing

[0059] Date of preservation: December 18, 2020

[0060] Registered with the China National Collection Center (CGMCC) No. 21507.

[0061] In this embodiment, the livestock and poultry manure used is cow manure and pig manure mixed in a mass ratio of 1:1.

[0062] In the example, the particle size of the rice husk powder is 0.4 mm.

[0063] In the example, the soybean meal powder has a particle size of 0.4 mm.

[0064] Example 1

[0065] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0066] The phosphate-solubilizing bacteria are a mixture of Aspergillus niger A44 and Rhizobium agapanthus in a mass ratio of 1:3.

[0067] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0068] Example 2

[0069] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0070] The phosphate-solubilizing bacteria are a mixture of Aspergillus niger A44 and Rhizobium agapanthus in a mass ratio of 3:1.

[0071] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0072] Example 3

[0073] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0074] The phosphate-solubilizing bacteria are a mixture of Aspergillus niger A44 and Rhizobium agapanthus in a mass ratio of 1:1.

[0075] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0076] Comparative Example 1

[0077] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0078] The phosphate-solubilizing bacteria is Aspergillus niger A44.

[0079] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0080] Comparative Example 2

[0081] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0082] The phosphate-solubilizing bacteria are *Agapanthus fasciatus* rhizobia.

[0083] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0084] Comparative Example 3

[0085] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0086] The phosphate-solubilizing bacteria is Aspergillus niger (China General Microbiological Culture Collection Center, accession number 3.3928).

[0087] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0088] Comparative Example 4

[0089] A high-efficiency phosphorus-solubilizing bio-organic fertilizer includes phosphorus-solubilizing bacteria and an organic substrate; 0.5 parts by weight of phosphorus-solubilizing bacteria, 20 parts by weight of livestock and poultry manure, 30 parts by weight of peat moss, 15 parts by weight of rice husk powder, and 10 parts by weight of soybean meal powder.

[0090] The phosphate-solubilizing bacteria were prepared by mixing Aspergillus niger (China General Microbiological Culture Collection Center, accession number 3.3928) and Rhizobium arachidii in a mass ratio of 1:1.

[0091] The preparation method of the high-efficiency phosphorus-soluble bio-organic fertilizer includes the following steps: the above-mentioned phosphorus-soluble bacteria, livestock and poultry manure, peat soil, rice husk powder and soybean meal powder are measured according to the production ratio, and then mixed evenly with a mixer to prepare powdered high-efficiency phosphorus-soluble bio-organic fertilizer.

[0092] Test Example 1

[0093] The high-efficiency phosphorus-soluble bio-organic fertilizer obtained in the examples was tested according to the requirements and testing methods of NY884-2012 "Bio-organic Fertilizer".

[0094] Table 1: Test Results of High-Efficiency Phosphorus-Soluble Bio-Organic Fertilizer

[0095] viable bacteria count (100 million / g) Organic matter / % Moisture / % Example 1 0.13 57.1 20 Example 2 0.40 60.3 21 Example 3 0.26 58.4 20 Comparative Example 1 0.09 53.3 19 Comparative Example 2 0.08 51.2 18 Comparative Example 3 0.05 49.5 19 Comparative Example 4 0.07 50.4 19

[0096] As can be seen from the results in Table 1 above, the high-efficiency phosphorus-soluble bio-organic fertilizer prepared in this application meets the requirements of NY884-2012 "Bio-organic Fertilizer".

[0097] Test Example 2

[0098] Crops: Corn "Denghai 605";

[0099] Fertilizer: The application rate of the high-efficiency phosphorus-soluble bio-organic fertilizer obtained in each example was 2600 kg / hm. 2 All fertilizers were applied as base fertilizer in a single application to the soil tillage layer, and no further topdressing was applied during the entire corn growing season. Other management practices remained consistent with local practices. A blank control group was set up, meaning no fertilizer was applied.

[0100] During the maize harvest season, ears of 30 consecutive plants with uniform growth were collected from each plot to determine aboveground biomass, maize yield, and yield components.

[0101] Table 2: Results of the Maize Planting Test

[0102]

[0103] This invention uses livestock and poultry manure, peat soil, rice husk powder, and soybean meal powder as raw materials, and Aspergillus niger and Rhizobium agapanthus as active biological bacteria to prepare high-efficiency phosphorus-soluble bio-organic fertilizer. It provides nutrients for crop growth, improves soil organic matter and soil aeration, increases microbial activity, thereby improving soil activity and effectively preventing soil compaction.

[0104] A comparison of Examples 1-3 and Comparative Examples 1-2 revealed that the planting effects of Examples 1-3 were superior to those of Comparative Examples 1-2. This is likely due to the introduction of *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia. *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia have different metabolic pathways and metabolites, which can interact to enhance phosphate solubility and improve phosphorus availability in the soil. For example, *Aspergillus niger* A44 can produce various organic acids (such as citric acid and malic acid), while *Agapanthus arachnoidea* rhizobia can produce substances such as IAA. These substances can promote phosphate decomposition and improve phosphorus availability in the soil. *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia exist in different ecological environments. When they act together on the soil, they can alter the soil environment, thereby affecting phosphate transformation. For example, *Aspergillus niger* A44 can lower the soil pH, making the soil environment more acidic, thus promoting the release of phosphate and its conversion into a usable form. Under different growth conditions, *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia may produce different metabolites, thus affecting phosphate solubility and improving soil phosphorus availability. For example, when *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia act together, they may produce more enzymes such as phosphatases and nucleases, thereby promoting phosphate decomposition and improving soil phosphorus availability. When *Aspergillus niger* A44 and *Agapanthus arachnoidea* rhizobia act together, they may exhibit synergistic effects, such as mutual stimulation and synergistic metabolism. These effects may lead to a synergistic enhancement of their phosphate-solubilizing properties, potentially promoting cell adsorption and phosphate decomposition, thereby improving soil phosphorus availability. Furthermore, they may also produce some synergistic metabolites, which may interact to enhance phosphate solubility and improve soil phosphorus availability.

[0105] Furthermore, by observing the data from Comparative Examples 3 and 4, it can be concluded that not all Aspergillus niger and Rhizobium agapanthus in the rhizosphere produce a synergistic effect when they work together, and the mechanism needs further investigation.

Claims

1. A high-efficiency phosphorus-solubilizing bio-organic fertilizer, comprising phosphorus-solubilizing bacteria and an organic substrate, characterized in that, The phosphate-solubilizing bacteria consist of Aspergillus niger and Rhizobium arachidii rhizobia in a mass ratio of 1:10 to 10:

1. The Aspergillus niger mentioned is Aspergillus niger A44, accession number: CGMCC No. 11793; The rhizobium of Agapanthus is Rhizobium rhizagapanthus 3T7, accession number: CGMCC No. 21507.

2. The high-efficiency phosphorus-soluble bio-organic fertilizer as described in claim 1, characterized in that: The high-efficiency phosphorus-soluble bio-organic fertilizer contains 0.1-0.4 billion live bacteria per gram.

3. The high-efficiency phosphorus-soluble bio-organic fertilizer as described in claim 1, characterized in that: The organic matrix is ​​at least one of the following: livestock and poultry manure, decomposed straw, peat moss, rice husk powder, wheat husk powder, and soybean meal powder.

4. The high-efficiency phosphorus-soluble bio-organic fertilizer as described in claim 1, characterized in that: The high-efficiency phosphorus-soluble bio-organic fertilizer also includes one or more additives, binders, and protectants.

5. The method for preparing high-efficiency phosphorus-soluble bio-organic fertilizer according to any one of claims 1-4, characterized in that: Includes the following steps: The above-mentioned phosphorus-solubilizing bacteria and organic matrix are measured according to the production ratio, and then mixed with a mixer to prepare powdered high-efficiency phosphorus-solubilizing bio-organic fertilizer; or the above-mentioned phosphorus-solubilizing bacteria, organic matrix, and binder are measured according to the production ratio, and then mixed with a mixer to prepare the high-efficiency phosphorus-solubilizing bio-organic fertilizer.

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