A nanofiber membrane for efficient and uniform delivery, slow release of collagen and a method of making the same

Abstract

The application provides a nanofiber membrane for efficiently and uniformly delivering and slowly releasing collagen and a preparation method thereof, the nanofiber membrane has a double-layer structure, the nanofiber membrane base layer is hydrophilic, the nanofiber membrane surface layer is hydrophobic, and collagen mist beads or collagen fiber filaments are distributed in the nanofiber membrane base layer. The fiber membrane has a base layer and a surface layer structure, the base layer is rich in a higher amount of collagen, and meanwhile, the hydrophilic polymer material is used to deliver collagen protein by means of the electrospinning technology for nanofiber structure, so as to achieve the purpose of efficiently and uniformly dispersing collagen to the skin; the hydrophobic polymer material is used to prepare the surface layer of the nanofiber membrane by means of the electrospinning technology, the collagen protein skin area is effectively covered, a waterproof and breathable layer is formed, water loss to the outside is prevented, a moist environment is provided at the same time with breathability, the skin can freely breathe, and the absorption of collagen protein by the skin is promoted.

Description

Technical Field

[0001] This invention relates to the field of nanofiber materials technology, and in particular to a nanofiber membrane for efficient and uniform delivery and slow release of collagen, and its preparation method. Background Technology

[0002] Collagen is a major structural protein in humans and vertebrates. As a key component of supporting and connective tissues, it plays multiple roles, including support, protection, and connection. In human skin, collagen accounts for 70%-80% of the dermis and is the main component that maintains the elasticity of skin and muscles, keeping the skin firm and elastic.

[0003] Based on the functionalities demonstrated in current collagen research, it is widely used in healthcare, beauty care, and other fields. The triple helix conformation of collagen endows it with physicochemical support properties and biological activity, enabling it to promote cell growth, adhesion, and synergistic wound repair with new cells and tissues when used as an artificial organ framework or wound dressing. This makes collagen a widely applicable biomedical material. Its sources mainly include animal-derived and recombinant collagen. However, animal-derived collagen still suffers from poor biocompatibility, making recombinant collagen more commonly used. The most well-known recombinant collagens in the medical and beauty industries include recombinant humanized type I collagen and recombinant humanized type III collagen.

[0004] The most common ways to apply collagen in practice include subcutaneous injection, ingestion, and topical application. In skin wound repair and skin rejuvenation, topical application is most common. This requires a relatively moist environment for skin absorption of collagen. However, when encapsulating collagen in wound repair, gauze is typically used, which doesn't effectively prevent moisture loss and maintain a relatively moist environment to promote collagen absorption. Furthermore, most skin rejuvenation products contain relatively low levels of pure collagen in their collagen solutions, and typical application methods fail to effectively and evenly distribute the collagen on the skin, significantly reducing the efficiency of collagen use.

[0005] Therefore, there are two problems in reality: (1) a relatively humid environment is needed to prevent moisture loss and promote the absorption of collagen by the skin; (2) the content of pure collagen is low, and the application method cannot make it evenly dispersed on the skin. How to efficiently distribute collagen evenly on the skin and maintain moisture for a long time remains to be solved.

[0006] In view of this, it is necessary to improve the existing collagen-rich carriers in order to solve the above problems. Summary of the Invention

[0007] The objective of this invention is to disclose a nanofiber membrane for efficient and uniform delivery and slow release of collagen. This membrane disperses and binds collagen onto a polymer nanofiber membrane, allowing the collagen to be efficiently and uniformly delivered to the skin surface with the help of the polymer material, while maintaining a relatively moist environment, thus prolonging the effect time of the collagen and improving its absorption rate.

[0008] To achieve the above objectives, the present invention provides a nanofiber membrane for efficient and uniform delivery and slow release of collagen. The nanofiber membrane has a bilayer structure, wherein the base layer of the nanofiber membrane is hydrophilic and the surface layer of the nanofiber membrane is hydrophobic, and collagen droplets or collagen filaments are distributed in the base layer of the nanofiber membrane.

[0009] In some embodiments, the collagen beads and collagen filaments are nanofiber bead / filament structures.

[0010] The second objective of this invention is to disclose a method for preparing a nanofiber membrane that efficiently and uniformly delivers and slowly releases collagen, wherein the collagen-rich membrane is coated onto the skin for direct use via electrostatic spraying and / or electrospinning.

[0011] To achieve the above objectives, this invention provides a method for preparing a nanofiber membrane with highly efficient and uniformly dispersed collagen, comprising the following steps:

[0012] Step 1: Prepare collagen mist bead nano-base solution or collagen fiber nano-base spinning solution;

[0013] Step 2: Prepare the spinning solution for the nanofiber membrane surface;

[0014] Step 3: Prepare collagen mist bead nano-base layer or collagen fiber filament nano-base layer;

[0015] Step 4: Based on Step 3, prepare the nanofiber membrane surface layer to form a nanofiber membrane that efficiently and uniformly delivers and slowly releases collagen.

[0016] In some embodiments, in step three, collagen mist drop nanolayers or collagen fiber nanolayers can be prepared, or both types of layers can be prepared simultaneously to deliver collagen.

[0017] In some embodiments, the collagen mist bead nano-base liquid is prepared by adding 10-15 parts of polyvinylpyrrolidone (PVP) and 1-3 parts of collagen to 80-90 parts of purified water, stirring at high speed until homogeneous, and then setting aside for use. The viscosity of the collagen mist bead nano-base liquid is 300-1500 mPa·s.

[0018] In some embodiments, the method for preparing the collagen fiber nano-base spinning solution is to mix 40-45 parts of purified water and 40-45 parts of anhydrous ethanol evenly, then add 18-25 parts of polyvinylpyrrolidone (PVP) and 1-3 parts of collagen, stir at high speed until evenly mixed, and set aside for use. The viscosity of the collagen fiber nano-base spinning solution is 450-2000 mPa·s.

[0019] In some embodiments, the spinning solution for the nanofiber membrane surface is prepared by adding 5-20 parts of polyvinyl butyral (PVB) or polyvinyl formal to 60-120 parts of anhydrous ethanol, stirring at high speed at room temperature until completely dissolved, and then adding 3-15 parts of medical silica gel dropwise to the completely dissolved solution while stirring until evenly dispersed before use.

[0020] In some embodiments, the collagen mist nanolayer is prepared by electrostatic spraying, wherein the electrostatic spraying conditions are a voltage of 6.5-12.5kV, a receiving distance of 5-15cm, and a propulsion speed of 1-5mL / h.

[0021] In some embodiments, the collagen fiber nanolayer is prepared by electrospinning, wherein the electrospinning working conditions are a voltage of 7-20kV, a receiving distance of 8-19cm, and a propulsion speed of 1-10mL / h.

[0022] In some embodiments, the nanofiber membrane surface layer is prepared by electrospinning, wherein the electrospinning operating conditions are a voltage of 8.5–20 kV, a collection distance of 10–20 cm, and a propulsion speed of 1–10 mL / h.

[0023] Compared with the prior art, the beneficial effects of the present invention are: (1) The fiber membrane has a base layer and a surface layer structure. The base layer is rich in collagen. At the same time, the hydrophilic polymer material is used to deliver collagen to the skin by electrospinning technology. This achieves the purpose of efficiently and evenly dispersing collagen to the skin. (2) The hydrophobic polymer material is used to prepare the nanofiber structure membrane by electrospinning technology. This effectively covers the collagen skin area, forming a waterproof and breathable layer. This prevents the internal moisture from being lost to the outside and provides a humid environment while being breathable, allowing the skin to breathe freely and promoting the absorption of collagen by the skin. Attached Figure Description

[0024] Figure 1 This is a diagram of the collagen mist droplet nano-base structure shown in this invention;

[0025] Figure 2 This is a diagram of the collagen fiber nanofiber base layer structure shown in this invention;

[0026] Figure 3This is a diagram illustrating the hydrophobic effect of the nanofiber membrane shown in this invention.

[0027] Figure 4 This is an image showing the effect of the hydrophobic and moisturizing membrane of the nanofiber membrane shown in this invention. Detailed Implementation

[0028] The present invention will now be described in detail with reference to the embodiments shown in the accompanying drawings. However, it should be noted that these embodiments are not intended to limit the present invention. Equivalent changes or substitutions in function, method, or structure made by those skilled in the art based on these embodiments are all within the scope of protection of the present invention.

[0029] like Figure 1-4 As shown, a nanofiber membrane for efficient and uniform delivery and slow release of collagen is disclosed. The nanofiber membrane has a bilayer structure, wherein the base layer is hydrophilic and the surface layer is hydrophobic. Collagen droplets or collagen filaments are distributed in the base layer. The collagen droplets and collagen filaments are nanofiber bead / filament structures.

[0030] This embodiment also discloses a method for preparing a nanofiber membrane that efficiently and uniformly delivers and slowly releases collagen. The collagen-rich membrane is coated onto the skin for direct use via electrostatic spraying and / or electrospinning, and includes the following steps:

[0031] Step 1: Prepare collagen mist bead nano-base solution or collagen fiber nano-base spinning solution;

[0032] Step 2: Prepare the spinning solution for the nanofiber membrane surface;

[0033] Step 3: Prepare collagen mist bead nano-base layer or collagen fiber filament nano-base layer;

[0034] Step 4: Based on Step 3, prepare the nanofiber membrane surface layer to form a nanofiber membrane that efficiently and uniformly delivers and slowly releases collagen.

[0035] In step three, collagen mist nanolayers or collagen fiber nanolayers can be prepared, or both types of layers can be prepared simultaneously for collagen delivery. The purpose of this step is to ensure that the nanofiber membrane contains as much collagen as possible in its base layer, which is then delivered to the skin surface while maintaining its moisture.

[0036] The collagen mist drop nano-base layer is made from an electrostatic spray dispersion. The preparation method for the collagen mist drop nano-base layer solution is to add 10-15 parts polyvinylpyrrolidone (PVP) and 1-3 parts collagen to 80-90 parts purified water, stir at high speed until homogeneous, and then set aside. The stirring speed is 800-1500 rpm, and the viscosity of the prepared electrostatic spray dispersion is 300-1500 mPa·s.

[0037] The preparation method of the collagen fiber nano-base spinning solution is to mix 40-45 parts of purified water and 40-45 parts of anhydrous ethanol evenly, then add 18-25 parts of polyvinylpyrrolidone (PVP) and 1-3 parts of collagen, stir at high speed until evenly mixed, and set aside for use. The viscosity of the collagen fiber nano-base spinning solution is 450-2000 mPa·s.

[0038] The method for preparing the spinning solution for the nanofiber membrane surface layer is as follows: add 5-20 parts of polyvinyl butyral (PVB) or polyvinyl formal to 60-120 parts of anhydrous ethanol, stir at high speed at room temperature until completely dissolved (stirring speed 1000-1500 rpm), then add 3-15 parts of medical silica gel dropwise to the completely dissolved solution while stirring until evenly dispersed, and set aside for use (stirring speed 1200-1500 rpm).

[0039] The collagen mist nano-base layer is prepared by electrostatic spraying. The working conditions of the electrostatic spraying are a voltage of 6.5-12.5kV, a receiving distance of 5-15cm, and a propulsion speed of 1-5mL / h.

[0040] The collagen fiber nanolayer is prepared by electrospinning. The working conditions for electrospinning are a voltage of 7-20kV, a receiving distance of 8-19cm, and a propulsion speed of 1-10mL / h.

[0041] The nanofiber membrane surface layer is prepared by electrospinning. The working conditions for electrospinning are a voltage of 8.5-20 kV, a collection distance of 10-20 cm, and a propulsion speed of 1-10 mL / h.

[0042] Working principle: Electrostatic spray dispersion and / or electrospinning dispersion, together with a portable electrospinning dressing gun, disperse collagen in situ on the skin surface; electrostatic spraying and electrospinning can be performed sequentially or simultaneously. After visually observing that the target skin area has been completely covered with a thin layer (i.e., collagen mist nano-base layer and / or collagen fiber nano-base layer) (a 10cm*10cm target area can be completely covered in about 5 minutes, depending on the actual use), a hydrophobic moisturizing coating (i.e., the surface layer of the nanofiber membrane) spinning solution, together with a portable electrospinning dressing gun, is applied to the target skin area for coating and moisturizing.

[0043] The vapor transmission rate of the fabricated nanofiber membrane was tested according to YY / T 0471.2-2004, and the test results are shown in Table 1. The higher the water vapor transmission rate, the greater the porosity of the fiber membrane.

[0044] Table 1. Water vapor transmission rate after nanofiber material coating

[0045] sample Water vapor transmission rate (g / m2·24h) 1 912 2 876 3 934 4 923 5 906 average value 910

[0046] Table 1 shows the breathability of the hydrophobic moisturizing film. Figure 3 The demonstration of the nanofiber membrane's external waterproofness indicates that while preventing internal moisture loss, the nanofiber membrane also ensures high breathability, allowing the skin to breathe freely.

[0047] If the nanofiber membrane is used for infusion patches or sterile wound dressings, it should be tested in accordance with the requirements of YY / T0148-2006 "General Requirements for Medical Tape".

[0048] The detailed descriptions listed above are merely specific descriptions of feasible embodiments of the present invention, and are not intended to limit the scope of protection of the present invention. All equivalent embodiments or modifications made without departing from the spirit of the present invention should be included within the scope of protection of the present invention.

[0049] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for preparing a nanofiber membrane for efficient and uniform delivery and slow release of collagen, characterized in that, The nanofiber membrane has a bilayer structure, the base layer of the nanofiber membrane is hydrophilic, the surface layer of the nanofiber membrane is hydrophobic, and collagen droplets and / or collagen filaments are distributed in the base layer of the nanofiber membrane; the collagen droplets and collagen filaments are nanofiber bead / filament structure; The preparation method includes the following steps: Step 1: Prepare collagen mist bead nano-base solution or collagen fiber nano-base spinning solution; The collagen mist nano-base solution comprises polyvinylpyrrolidone (PVP), collagen, and purified water; the collagen fiber nano-base spinning solution comprises polyvinylpyrrolidone (PVP), collagen, purified water, and anhydrous ethanol. Step 2: Prepare the spinning solution for the nanofiber membrane surface; The spinning solution on the surface of the nanofiber membrane includes polyvinyl butyral (PVB) or polyvinyl formal, anhydrous ethanol and medical-grade silicone. Step 3: Preparation of collagen mist nano-base layer or collagen fiber nano-base layer; The collagen mist nano-base layer is prepared by electrostatic spraying, with the operating conditions being a voltage of 6.5-12.5kV, a receiving distance of 5-15cm, and a propulsion speed of 1-5mL / h; The collagen fiber nano-base layer is prepared by electrospinning, with the operating conditions being a voltage of 7-20kV, a receiving distance of 8-19cm, and a propulsion speed of 1-10mL / h; The collagen mist nano-base layer and / or the collagen fiber nano-base layer are then dispersed in situ on the skin surface using a portable electrospinning dressing gun for in-situ dispersion of collagen; The preparation method of the collagen mist nano-base liquid is to add 10-15 parts of polyvinylpyrrolidone (PVP) and 1-3 parts of collagen to 80-90 parts of purified water, stir at high speed until uniform, and set aside for use. The viscosity of the collagen mist nano-base liquid is 300-1500 mPa·s. The preparation method of the collagen fiber nano-base spinning solution is to mix 40-45 parts of purified water and 40-45 parts of anhydrous ethanol evenly, add 18-25 parts of polyvinylpyrrolidone (PVP) and 1-3 parts of collagen, stir at high speed until evenly mixed, and set aside for use. The viscosity of the collagen fiber nano-base spinning solution is 450-2000 mPa·s. The method for preparing the spinning solution for the nanofiber membrane surface layer is to add 5-20 parts of polyvinyl butyral (PVB) or polyvinyl formal to 60-120 parts of anhydrous ethanol, stir at high speed at room temperature until completely dissolved, and then add 3-15 parts of medical silica gel dropwise to the completely dissolved solution while stirring until evenly dispersed before use. Step 4: Based on Step 3, prepare a nanofiber membrane surface layer to form a nanofiber membrane that efficiently and uniformly delivers and slowly releases collagen. The nanofiber membrane surface layer is prepared by electrospinning. The working conditions for electrospinning are a voltage of 8.5~20 kV, a collection distance of 10~20 cm, and a propulsion speed of 1~10 mL / h. The nanofiber membrane surface layer is applied to the target skin area for moisturizing using a portable electrospinning dressing gun.

2. The method for preparing a nanofiber membrane for efficient and uniform delivery and slow release of collagen according to claim 1, characterized in that, In step three, collagen mist drop nano-base layer or collagen fiber nano-base layer can be prepared, or both types of base layers can be prepared simultaneously to deliver collagen.

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