A method for preparing rice and pea plant double protein

Abstract

The invention provides a method for preparing rice and pea plant double protein, comprising the following steps: using rice protein as a raw material, adding water to prepare a rice protein raw material slurry, adjusting pH, homogenizing, and adding pea protein powder after high temperature shearing, preparing a plant double protein slurry containing pea protein powder, adjusting pH, homogenizing, and spray drying after high temperature shearing to obtain rice and pea plant double protein. The rice and pea plant double protein powder prepared by the preparation method of the invention is based on the complementary and coordinated advantages of rice protein and pea protein amino acids. Compared with a single enzymatically hydrolyzed rice protein, an enzymatically hydrolyzed pea protein, or a direct mixture of the enzymatically hydrolyzed protein powders of the two, the rice and pea plant double protein powder has a smooth and delicate taste, a maximum nitrogen solubility index of more than 85%, and good emulsification, foaming and other functional characteristics, a higher plant double protein digestibility, and improved nutritional value, thereby expanding the application range of plant protein.

Description

Technical Field

[0001] The invention relates to the technical field of plant protein product processing, and in particular to a method for preparing rice and pea plant double proteins. Background Art

[0002] Protein is one of the most basic substances for human activities. With the continuous growth of population, the demand for protein is also gradually increasing. However, the production of large amounts of animal protein will cause problems such as excessive consumption of resources and damage to the ecological environment. The processing cost of animal protein is high and there are safety risks. Excessive consumption of animal protein can cause chronic diseases and other diseases. Plant protein mainly comes from rice, flour and beans. It has the advantages of being cholesterol-free, low-fat, nutritious, safe, widely available, low-cost and environmentally friendly. Compared with animal protein, it has different nutritional effects and is best consumed in proportion.

[0003] Pea is one of the most widely planted and eaten legumes. As an important agricultural commodity, it has high dietary fiber and protein, low sodium, low fat, high lysine content and nutritional value, and low allergenicity. At present, the domestic processing of peas is mainly concentrated on the production of pea starch and other related products, and the processing and utilization of protein resources are relatively small, resulting in a waste of protein resources. Rice protein is mainly derived from the by-products of rice starch processing, and is composed of albumin (2%-5%), globulin (2%-10%), glutenin (66%-78%) and alcohol-soluble protein (1%-5%). Rice protein is the protein with the highest biological value among all grains (77). In addition, rice protein is a low-allergenic plant protein with high digestibility and net protein utilization. Rice protein is a rigid globular structure protein. The subunits form dense molecular aggregates through intramolecular and intermolecular disulfide bonds and hydrophobic interactions. It has poor solubility, which limits its application in the food field.

[0004] At present, double protein is mainly directly mixed in the form of pea protein and rice protein powder, or the two protein mixed powders are enzymatically modified, directly achieving the advantage of amino acid balance and complementary improvement of its nutritional value, and the strong granularity of protein powder seriously affects its taste and product promotion and application. As in the patent "A preparation method of plant double protein and its product" (publication number CN111493208A), the patent mainly uses pea protein and rice protein as raw materials, performs sizing, enzymolysis, mixing, enzyme synthesis to prepare protein-like reaction, enzyme inactivation and drying, etc.; the operation process is complicated and cumbersome. The present invention processes its slurry to change its structure and then improve its taste, promoting its application in different fields.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The object of the present invention is to provide a method for preparing rice and pea plant double protein, which is simple to operate, short in time and low in cost, and avoids the defects of complex process with poor effect, destruction of nutrients, and indirect impact on the quality of plant double protein.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The invention provides a method for preparing rice and pea plant double protein. The method comprises the following steps: taking rice protein as a raw material, adding water to prepare rice protein raw material slurry, adjusting pH, homogenizing, high-temperature shearing, and then adding pea protein powder to prepare plant double protein slurry containing pea protein powder, adjusting pH, homogenizing, high-temperature shearing, and spray drying to prepare rice and pea plant double protein.

[0009] Further, the solid content of the rice protein raw material slurry is 6-20%;

[0010] And / or, the solid content of the plant double protein slurry is 6-20%.

[0011] Further, the dry basis protein content of the rice protein is 80-85%;

[0012] And / or, the dry basis protein content of the pea protein powder is greater than 80%.

[0013] Further, the pH value of the rice protein raw material slurry is adjusted to 6.0-11.5;

[0014] And / or, the pH value of the plant double protein slurry is adjusted to 6.0-11.5.

[0015] Further, the homogenization pressure of the rice protein raw material slurry is 30-55MPa;

[0016] And / or, the rice protein raw material slurry is homogenized 1 to 2 times.

[0017] Further, the temperature of the high temperature shearing of the rice protein raw material slurry is set to 130-170° C.; and / or the high temperature shearing time of the rice protein raw material slurry is 30-60s; and / or the flow rate of the high temperature shearing of the rice protein raw material slurry is 1-3T / h.

[0018] Furthermore, the dry basis mass ratio of the pea protein powder to the rice protein is 6:(1-7).

[0019] Furthermore, the homogenization pressure of the plant double protein slurry is 30-55MPa;

[0020] And / or, the plant double protein slurry is homogenized 1 to 3 times.

[0021] Further, the temperature of the high temperature shearing of the plant double protein slurry is set to 130-170°C; and / or, the time of the high temperature shearing of the plant double protein slurry is set to 30-60s; and / or, the flow rate of the high temperature shearing of the plant double protein slurry is 1-3T / h.

[0022] Furthermore, the spray drying inlet temperature is 135-185°C, and the outlet temperature is 55-85°C.

[0023] Compared with the prior art, the technical solution of the present invention has at least the following technical effects:

[0024] The present invention mixes the rice protein slurry passed through the high temperature shearing device with the pea protein powder, and then processes it through the high temperature shearing device again to modify the physical structure, and obtains the rice and pea plant double protein powder after spray drying. The rice and pea plant double protein powder prepared by the present invention is based on the complementary and coordinated advantages of rice protein and pea protein amino acids. Compared with a single enzymatically hydrolyzed rice protein, an enzymatically hydrolyzed pea protein or a direct mixture of the enzymatically hydrolyzed protein powders of the two, the rice and pea plant double protein powder has a smooth and delicate taste, a maximum nitrogen solubility index of more than 85%, and good emulsification, foaming and other functional characteristics, a higher plant double protein digestibility, and improved nutritional value, thereby expanding the application range of plant protein. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The drawings constituting a part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. Among them:

[0026] Figure 1 This is a schematic diagram of the structure of the shearing equipment used in high-temperature shearing of the present invention;

[0027] Figure 2 This is a top view of the structure of the rotating drum proposed by the present invention.

[0028] Explanation of the reference numerals: 1. Silo; 2. Evaporator; 3. Rotating drum; 4. Annular channel; 5. Feed pipe; 6. Steam pipeline; 31. Through hole. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical scheme and advantages of the present invention clearer, the technical scheme in the embodiment of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention. The process parameters of the following embodiments that do not specify specific conditions are usually based on conventional conditions.

[0030] The endpoints and any values ​​of the ranges disclosed in the present invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be regarded as specifically disclosed in the present invention.

[0031] According to a first aspect of the present invention, there is provided a method for preparing rice and pea plant double protein, comprising the following steps:

[0032] Rice protein is used as a raw material, water is added to prepare a rice protein raw material slurry, the pH value is adjusted, homogenized, high-temperature shearing is performed, and then pea protein powder is added to prepare a plant double protein slurry containing pea protein powder, the pH value is adjusted, homogenized, high-temperature shearing is performed, and then spray drying is performed to obtain rice and pea plant double protein.

[0033] Rice protein and pea protein have different protein structures. A large amount of gluten exists in rice protein. In the present invention, the rice protein raw material slurry is homogenized and pre-treated by high-temperature shearing, and then the pea protein is added to make the two proteins better fused, thereby improving the performance and taste of the plant double protein.

[0034] In the preparation method of above-mentioned rice and pea plant double protein, as a kind of preferred embodiment, the solid content of described rice protein raw material slurry is 6-20% (such as 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%); Preferably, the solid content of described plant double protein slurry is 6-20% (such as 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%). According to the water absorption characteristics of albumen, the solid content of slurry directly affects the spray drying effect, solid is too high, and material fluidity variation is difficult to carry out spray drying, and solid is too low and can reduce spray drying milling efficiency and increase cost, therefore determines the best solid concentration of the present invention.

[0035] In the preparation method of above-mentioned rice and pea plant biprotein, as a kind of preferred embodiment, the dry basis protein content of described rice protein is 80-85% (such as 80%, 81%, 82%, 83%, 84%, 85%).The dry basis protein content of rice protein among the present invention is preferably controlled within this scope, if dry basis protein content is too low, then can cause the content of final product protein to be too low, and if want to obtain too high dry basis protein content then need to increase production process, can cause the increase of cost.

[0036] In the above-mentioned method for preparing rice and pea plant dual proteins, as a preferred embodiment, the rice protein includes any one of protein residues or low-soluble rice protein; preferably, the protein residue is produced when rice starch sugar is produced from rice or broken rice.

[0037] In the preparation method of above-mentioned rice and pea plant double protein, as a kind of preferred embodiment, described low soluble rice protein is selected from at least one of spray rice protein, tube bundle rice protein or airflow rice protein.Solubility is mainly based on the mensuration of nitrogen solubility index, and the nitrogen solubility index of the rice protein that is only dried by spraying, tube bundle and airflow mode without modification is usually less than 5%, so it is labeled as low soluble rice protein.

[0038] In the above-mentioned method for preparing rice and pea plant double protein, as a preferred embodiment, the pH value of the rice protein raw material slurry is adjusted to 6.0-11.5 (such as 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, 10.5, 11.0, 11.5).

[0039] In the preparation method of the above-mentioned rice and pea plant double protein, as a preferred embodiment, the homogenization pressure of the rice protein raw material slurry is 30-55MPa (such as 30MPa, 35MPa, 40MPa, 45MPa, 50MPa, 55MPa); Too low homogenization pressure can cause the material particle size to be uneven, affecting product quality, and too high homogenization pressure can affect the particle size, form and aggregation state of protein, so the homogenization pressure of the rice protein raw material slurry of the present invention is preferably controlled within this range. Preferably, the homogenization number of times is 1-2 times.

[0040] In the preparation method of above-mentioned rice and pea plant biprotein, as a kind of preferred embodiment, the temperature setting of the high temperature shear of described rice protein raw material slurry is 130-170 ℃ (such as 130 ℃, 140 ℃, 150 ℃, 160 ℃, 170 ℃), the time is set to 30-60s (such as 30s, 40s, 50s, 60s), and the flow velocity is 1-3T / h (such as 1T / h, 2T / h, 3T / h). The setting of the time, flow velocity and temperature of the high temperature shear of rice protein raw material slurry is preferably controlled within the scope of the present invention, if departing from the preferred range, then can directly affect protein structure, thereby affect its mouthfeel and color and luster.

[0041] In the above-mentioned method for preparing rice and pea plant dual proteins, as a preferred embodiment, the dry basis protein content of the pea protein powder is greater than 80%. The dry basis protein content of the pea protein powder is greater than 80%, which can ensure that the protein content of the final product is greater than 80%.

[0042] In the above-mentioned preparation method of rice and pea plant double protein, as a preferred embodiment, the dry basis mass ratio of the pea protein powder and the rice protein is 6: (1-7) (such as 6: 1, 6: 2, 6: 3, 6: 4, 6: 5, 6: 6, 6: 7). The amino acid composition of rice protein and pea protein is different. After the complementary matching of the two proteins, a reasonable amino acid ratio can be obtained, providing a higher quality plant protein source. If it exceeds the preferred ratio range of the present invention, it will cause amino acid imbalance and the nutritional value will be affected.

[0043] In the above-mentioned method for preparing rice and pea plant dual protein, as a preferred embodiment, the pH value of the plant dual protein slurry is adjusted to 6.0-11.5 (such as 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0, 10.5, 11.0, 11.5).

[0044] In the above-mentioned method for preparing rice and pea plant dual protein, as a preferred embodiment, the homogenization pressure of the plant dual protein slurry is 30-55MPa (such as 30MPa, 35MPa, 40MPa, 45MPa, 50MPa, 55MPa), preferably, the homogenization times is 1 to 3 times (such as 1 time, 2 times, 3 times).

[0045] In the above-mentioned method for preparing rice and pea plant dual proteins, as a preferred embodiment, the temperature of the high-temperature shearing of the plant dual protein slurry is set to 130-170°C (such as 130°C, 140°C, 150°C, 160°C, 170°C), the time is set to 30-60s (such as 30s, 40s, 50s, 60s), and the flow rate is 1-3T / h (such as 1T / h, 2T / h, 3T / h).

[0046] In the above-mentioned method for preparing rice and pea plant dual protein, as a preferred embodiment, the spray drying inlet temperature is 135-185°C (such as 135°C, 145°C, 155°C, 165°C, 175°C, 185°C), and the outlet temperature is 55-85°C (such as 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C).

[0047] In addition, the high temperature shearing of the raw material slurry (such as rice protein raw material slurry, plant double protein slurry) described in the present invention can be carried out by the following method: Figure 1 , 2The shearing device shown in the figure is carried out, and the shearing device includes a silo 1 and an evaporator 2, and the outlet of the silo 1 is connected to the evaporator 2; a rotating drum 3 is arranged inside the silo 1, and an annular channel 4 is formed between the inner wall of the silo 1 and the outer wall of the rotating drum 3; a feeding pipe 5 is arranged on the side wall of the silo 1, and the discharge port of the feeding pipe 5 is connected to the annular channel 4, and the discharge direction is tangent to the side wall of the rotating drum 3;

[0048] A steam pipeline 6 is provided on the top of the rotating drum 3, and the air outlet of the steam pipeline 6 is connected to the interior of the rotating drum 3; a through hole 31 is opened on the side wall of the rotating drum 3; the material passes through the feed pipe 5 and quickly enters the annular channel 4 along the tangent direction of the side wall of the rotating drum 3. The rotating drum 3 starts to rotate rapidly under the thrust of the material, and the material forms a thin film layer on the inner wall of the bin body 1. At the same time, the pressurized saturated steam quickly contacts the material from the inside of the rotating drum 3 through the through hole 31. The material quickly heats up and then quickly extends. The dispersed steam shears the material in an orderly manner. The sheared material enters the evaporator 2 to remove part of the water. The evaporator 2 plays a role in concentrating the sheared liquid.

[0049] The temperature of high temperature shear refers to the temperature of the steam entering the shearing equipment;

[0050] The high temperature shearing time refers to the length of time the material is in contact with the steam;

[0051] The flow rate of high temperature shear refers to the flow rate of the feed at the feed inlet.

[0052] The present invention is further described in detail below in conjunction with specific examples and comparative examples. It should be noted that the equipment used for spray drying in the following examples and comparative examples 1-14 is a spray drying tower, and the equipment used for high temperature shearing in the examples and comparative examples 1-13 is the above-mentioned shearing equipment, as shown in the attached Figure 1 , 2 shown.

[0053] Example 1

[0054] This embodiment provides a method for preparing rice and pea plant double protein, comprising the following steps:

[0055] Rice residues generated when rice or broken rice is used to produce rice starch sugar, or low-soluble rice protein is used as a raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, the slurry is homogenized, and pea protein powder is added to the slurry by adopting high-temperature shearing technology to prepare a plant double protein slurry containing pea protein powder, the pH value is adjusted, the slurry is homogenized, and the rice and pea plant double protein is prepared by spray drying after high-temperature shearing.

[0056] The dry protein content of the protein residue obtained during the preparation of starch sugar is 81%.

[0057] The solid content of the rice protein raw material slurry is 16%.

[0058] The pH of the rice protein raw material slurry is adjusted to 7.5.

[0059] The homogenization pressure of the rice protein raw material slurry is 55MPa, and the homogenization times is 1 time.

[0060] The temperature of the rice protein using the high temperature shearing technology is set to 150°C.

[0061] The time for high temperature shearing of the rice protein is 45s.

[0062] The flow rate of the rice protein using high temperature shearing is 2 T / h.

[0063] The dry-based protein content of the pea protein is 82%.

[0064] The dry basis mass ratio of the pea protein to the rice protein is 6:4.

[0065] The solid content of the plant double protein slurry is 15%.

[0066] The pH of the plant double protein slurry is adjusted to 7.0.

[0067] The homogenization pressure of the plant double protein slurry is 55 MPa, and the homogenization frequency is 1 time.

[0068] The temperature of the plant double protein slurry is set to 170° C. using high temperature shearing technology.

[0069] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0070] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0071] The spray drying inlet temperature of the plant double protein slurry is 175°C, and the outlet temperature is 78°C.

[0072] Example 2

[0073] This embodiment provides a method for preparing rice and pea plant double protein, comprising the following steps:

[0074] Rice residues generated when rice or broken rice is used to produce rice starch sugar, or low-soluble rice protein is used as a raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, the slurry is homogenized, and pea protein powder is added to the slurry by adopting high-temperature shearing technology to prepare a plant double protein slurry containing the pea protein powder, and the pH value is adjusted, the slurry is homogenized, and the rice and pea plant double protein is prepared by spray drying after high-temperature shearing.

[0075] The rice protein is airflow protein, and its dry basis protein content is 80%.

[0076] The solid content of the rice protein raw material slurry is 12%.

[0077] The pH of the rice protein raw material slurry is adjusted to 10.5.

[0078] The rice protein raw material slurry is homogenized at a pressure of 45 MPa and the homogenization times are 2 times.

[0079] The temperature of the rice protein raw material slurry is set to 160° C. using high temperature shearing technology.

[0080] The rice protein raw material slurry is subjected to high temperature shearing for 30 seconds.

[0081] The flow rate of the rice protein raw material slurry using high temperature shearing is 3 T / h.

[0082] The dry-based protein content of the pea protein is 80%.

[0083] The dry basis mass ratio of the pea protein to the rice protein is 6:2.

[0084] The solid content of the plant double protein slurry is 13%.

[0085] The pH of the plant double protein slurry is adjusted to 8.5.

[0086] The homogenization pressure of the plant double protein slurry is 50MPa, and the homogenization frequency is 1 time.

[0087] The temperature of the plant double protein slurry is set to 160° C. using high temperature shearing technology.

[0088] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0089] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0090] The spray drying inlet temperature of the plant double protein slurry is 165°C, and the outlet temperature is 70°C.

[0091] Example 3

[0092] This embodiment provides a method for preparing rice and pea plant double protein, comprising the following steps:

[0093] Rice residues generated when rice or broken rice is used to produce rice starch sugar, or low-soluble rice protein is used as a raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, the slurry is homogenized, and pea protein powder is added to the slurry by adopting high-temperature shearing technology to prepare a plant double protein slurry containing the pea protein powder, and the pH value is adjusted, the slurry is homogenized, and the rice and pea plant double protein is prepared by spray drying after high-temperature shearing.

[0094] The rice protein is sprayed protein, and its dry basis protein content is 84%.

[0095] The solid content of the rice protein raw material slurry is 12%.

[0096] The pH of the rice protein raw material slurry is adjusted to 10.

[0097] The rice protein raw material slurry is homogenized at a pressure of 35 MPa and the homogenization times are 2 times.

[0098] The temperature of the rice protein raw material slurry is set to 175° C. using high temperature shearing technology.

[0099] The rice protein raw material slurry is subjected to high temperature shearing for 60 seconds.

[0100] The flow rate of the rice protein raw material slurry using high temperature shearing is 1 T / h.

[0101] The dry-based protein content of the pea protein is 85%.

[0102] The dry basis mass ratio of the pea protein to the rice protein is 6:2.

[0103] The solid content of the plant double protein slurry is 13%.

[0104] The pH of the plant double protein slurry is adjusted to 6.5.

[0105] The homogenization pressure of the plant double protein slurry is 50MPa, and the homogenization frequency is 1 time.

[0106] The temperature of the plant double protein slurry is set to 150° C. using high temperature shearing technology.

[0107] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0108] The flow rate of the plant double protein slurry using high temperature shearing is 1.5 T / h.

[0109] The spray drying inlet temperature of the plant double protein slurry is 180°C, and the outlet temperature is 80°C.

[0110] Comparative Example 1

[0111] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the method does not contain a homogenization treatment of a rice protein raw material slurry, and comprises the following steps:

[0112] Rice residues generated when rice or broken rice is used to produce rice starch sugar, or low-soluble rice protein is used as raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, pea protein powder is added to the slurry by adopting high-temperature shearing technology, a plant double protein slurry containing pea protein powder is prepared, the pH value is adjusted, homogenized, high-temperature shearing and then spray-dried to obtain rice and pea plant double protein.

[0113] The dry protein content of the protein residue obtained during the preparation of starch sugar is 81%.

[0114] The solid content of the rice protein raw material slurry is 16%.

[0115] The pH of the rice protein raw material slurry is adjusted to 7.5.

[0116] The temperature of the rice protein raw material slurry is set to 150° C. using the high temperature shearing technology.

[0117] The time for high temperature shearing of the rice protein raw material slurry is 45 seconds.

[0118] The flow rate of the rice protein raw material slurry using high temperature shearing is 2 T / h.

[0119] The dry-based protein content of the pea protein is 82%.

[0120] The dry basis mass ratio of the pea protein to the rice protein is 6:4.

[0121] The solid content of the plant double protein slurry is 15%.

[0122] The pH of the plant double protein slurry is adjusted to 7.0.

[0123] The homogenization pressure of the plant double protein slurry is 55 MPa, and the homogenization frequency is 1 time.

[0124] The temperature of the plant double protein slurry is set to 170° C. using high temperature shearing technology.

[0125] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0126] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0127] The spray drying inlet temperature of the plant double protein slurry is 175°C, and the outlet temperature is 78°C.

[0128] Comparative Example 2

[0129] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the method does not contain high temperature shearing treatment of rice protein raw material slurry, and comprises the following steps:

[0130] Rice residues generated when rice or broken rice is used to produce rice starch sugar or low-soluble rice protein is used as a raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, the slurry is homogenized, pea protein powder is added to the slurry, a plant double protein slurry containing the pea protein powder is prepared, the pH value is adjusted, the slurry is homogenized, and the rice and pea plant double protein is prepared after high-temperature shearing and spray drying.

[0131] The dry protein content of the protein residue obtained during the preparation of starch sugar is 81%.

[0132] The solid content of the rice protein raw material slurry is 16%.

[0133] The pH of the rice protein raw material slurry is adjusted to 7.5.

[0134] The rice protein raw material slurry is homogenized at a pressure of 55 MPa and the homogenization frequency is 1 time.

[0135] The dry-based protein content of the pea protein is 82%.

[0136] The dry basis mass ratio of the pea protein to the rice protein is 6:4.

[0137] The solid content of the plant double protein slurry is 15%.

[0138] The pH of the plant double protein slurry is adjusted to 7.0.

[0139] The homogenization pressure of the plant double protein slurry is 55 MPa, and the homogenization frequency is 1 time.

[0140] The temperature of the plant double protein slurry is set to 170° C. using high temperature shearing technology.

[0141] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0142] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0143] The spray drying inlet temperature of the plant double protein slurry is 175°C, and the outlet temperature is 78°C.

[0144] Comparative Example 3

[0145] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those in Example 1, except that the method does not contain homogenization of the plant double protein slurry, and comprises the following steps:

[0146] Rice residues generated when rice or broken rice is used to produce rice starch sugar, or low-soluble rice protein is used as raw material, water is added to adjust the rice protein raw material slurry, the pH value is adjusted, the slurry is homogenized, pea protein powder is added to the slurry by adopting high-temperature shearing technology, a plant double protein slurry containing pea protein powder is prepared, the pH value is adjusted, high-temperature shearing is performed, and spray drying is performed to obtain rice and pea plant double protein.

[0147] The dry protein content of the protein residue obtained during the preparation of starch sugar is 81%.

[0148] The solid content of the rice protein raw material slurry is 16%.

[0149] The pH of the rice protein raw material slurry is adjusted to 7.5.

[0150] The rice protein raw material slurry is homogenized at a pressure of 55 MPa and the homogenization frequency is 1 time.

[0151] The temperature of the rice protein raw material slurry is set to 150° C. using the high temperature shearing technology.

[0152] The time for high temperature shearing of the rice protein raw material slurry is 45 seconds.

[0153] The flow rate of the rice protein raw material slurry using high temperature shearing is 2 T / h.

[0154] The dry-based protein content of the pea protein is 82%.

[0155] The dry basis mass ratio of the pea protein to the rice protein is 6:4.

[0156] The solid content of the plant double protein slurry is 15%.

[0157] The pH of the plant double protein slurry is adjusted to 7.0.

[0158] The temperature of the plant double protein slurry is set to 160° C. using high temperature shearing technology.

[0159] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0160] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0161] The spray drying inlet temperature of the plant double protein slurry is 175°C, and the outlet temperature is 78°C.

[0162] Comparative Example 4

[0163] A method for preparing rice and pea plant double protein comprises the following steps: using rice residue produced when rice or broken rice is used to produce rice starch sugar or using low-soluble rice protein as raw material, adding water to prepare rice protein raw material slurry, adding pea protein powder to the slurry, preparing plant double protein slurry containing pea protein powder, adjusting pH, homogenizing, high-temperature shearing and then spray drying to prepare rice and pea plant double protein.

[0164] The dry protein content of the protein residue obtained during the preparation of starch sugar is 82%.

[0165] The solid content of the rice protein raw material slurry is 16%.

[0166] The dry-based protein content of the pea protein is 82%.

[0167] The dry basis mass ratio of the pea protein to the rice protein is 6:4.

[0168] The solid content of the plant double protein slurry is 15%.

[0169] The pH of the plant double protein slurry is adjusted to 7.0.

[0170] The homogenization pressure of the plant double protein slurry is 55 MPa, and the homogenization frequency is 1 time.

[0171] The temperature of the plant double protein slurry is set to 140° C. using high temperature shearing technology.

[0172] The plant double protein slurry is subjected to high temperature shearing for 30 seconds.

[0173] The flow rate of the plant double protein slurry using high temperature shearing is 3 T / h.

[0174] Comparative Example 5

[0175] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the steps do not contain high-temperature shearing treatment of the plant double protein slurry.

[0176] Comparative Example 6

[0177] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the homogenization pressure of the rice protein raw material slurry described in the steps is 25 MPa.

[0178] Comparative Example 7

[0179] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the temperature of the high-temperature shearing of the rice protein raw material slurry described in the steps is set to 120°C.

[0180] Comparative Example 8

[0181] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the temperature of the high-temperature shearing of the rice protein raw material slurry described in the steps is set to 180°C.

[0182] Comparative Example 9

[0183] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the rice protein raw material slurry described in the steps is subjected to high temperature shearing for 25 seconds at a flow rate of 0.8 T / h.

[0184] Comparative Example 10

[0185] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the homogenization pressure of the plant double protein slurry described in the steps is 25 MPa.

[0186] Comparative Example 11

[0187] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the plant double protein slurry described in the steps is subjected to high temperature shearing for 25 seconds at a flow rate of 0.8 T / h.

[0188] Comparative Example 12

[0189] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the temperature of the high-temperature shearing of the plant double protein slurry described in the steps is set to 120°C.

[0190] Comparative Example 13

[0191] A method for preparing rice and pea plant double protein, wherein the specific steps are substantially the same as those of Example 1, except that the temperature of the high-temperature shearing of the plant double protein slurry described in the steps is set to 180°C.

[0192] Comparative Example 14

[0193] A method for preparing a rice and pea plant double protein, wherein the specific steps are the same as those of Example 1, except that the shearing equipment used for the high-temperature shearing of the rice protein raw material slurry and the high-temperature shearing of the plant double protein slurry in the steps is a commercially available product (high-shear high-speed disperser AYF-220, Nantong Bolide Machinery Technology Co., Ltd.), and the shearing conditions are as follows: the shearing speed is 1400 rpm, the shearing temperature is room temperature, and the shearing time is 5 min.

[0194] Experimental Example 1

[0195] (1) Sensory evaluation: Take an appropriate amount of sample and place it in a white porcelain plate. Observe its color and state under natural light, check for impurities, smell its odor, rinse your mouth with warm water, and taste its flavor. Use a scoring method of 1-5 points. The higher the score, the better the taste. This includes the evaluation of color, state, odor, granularity, smoothness, and bitterness.

[0196] (2) Determination of nitrogen solubility index: Weigh 5 g of sample into a ground-mouth flask with a stopper, add 200 mL of water, and then place it in a 200 rpm, 30°C vortex water bath constant temperature oscillator for 2 hours. After the oscillation, transfer the sample solution in the flask to a 250 mL volumetric flask, cool it to room temperature, and then make up the volume and shake it for 5 minutes; then shake the sample solution and centrifuge it at 1500 r / min for 10 minutes. Filter with fast filter paper and set aside the filtrate. The protein content was determined by the Kjeldahl method according to GB 5009.5 2016.

[0197]

[0198] (3) Determination of emulsification and emulsion stability:

[0199] ① Weigh 2.5 g of sample, disperse it in 50 mL of water, add 50 mL of salad oil, stir in a tissue grinder for 1 min, pour an appropriate amount into a graduated centrifuge tube, centrifuge at 2500 rpm for 5 min, and record the liquid height and emulsion layer height in the graduated centrifuge tube respectively.

[0200]

[0201] ② Place the above centrifuge tube in an 80℃ water bath, heat for 30 minutes, cool to room temperature with tap water, centrifuge again for 5 minutes at 2500rpm, and record the height of the emulsion layer.

[0202]

[0203] (4) Determination of foaming property and foam stability: Weigh 8 g of protein sample, dissolve in 92 mL of water, pour into a vial, measure and record the height of the sample solution (H1). Use a high-speed shearing machine to shear the sample at 12,000 rpm for 5 minutes, and then immediately measure and record the height of the sample solution (H2). After 30 minutes, record the sample height (H3). The foaming capacity (FC) and foam stability (FS) of the sample are calculated as follows:

[0204] FC(%)=(H2-H1) / H1×100%

[0205] FS(%)=(H3-H1) / H1×100%

[0206] (5) Determination of water holding capacity: Weigh the protein sample with the marked mass as m1 and place it in a 10 mL centrifuge tube. The total weight of the sample and the centrifuge tube is m2. Add distilled water so that the water just covers the sample, shake for 5 minutes to mix it, centrifuge at 5000 rpm for 15 minutes, and then absorb the water on the top of the centrifuge tube with filter paper. Accurately weigh the total mass of the sample and the centrifuge tube, which is recorded as m3.

[0207]

[0208] (6) Determination of in vitro digestibility:

[0209] (1) Preparation of simulated fluid

[0210] ① Oral simulated fluid (SSF) consists of 15.1mL KCl, 3.7mL KH2PO4, 6.8mL NaHCO3, 0.5mL MgCl2(H2O)6, 0.06mL (NH4)2CO3, and 0.09mL HCl.

[0211] ② Gastric simulated fluid (SGF) consists of 0.9mL KH2PO4, 6.9mL KCl, 12.5mL NaHCO3, 11.8mL NaCl, 0.4mL MgCl2(H2O)6, 0.5mL (NH4)2CO3, and 1.3mL HCl.

[0212] ③Simulated intestinal fluid (SIF) consisted of 6.8 mL KCl, 0.8 mL KH2PO4, 42.5 mL NaHCO3, 9.6 mL NaCl, 1.1 mL MgCl2(H2O)6, and 0.7 mL HCl.

[0213] (2) Digestion Experiment Procedure

[0214] ① Simulated oral digestion: Weigh 0.5 g (accurate to 0.001 g) of rice pea protein powder, add 5 mL of deionized water, mix it with equal volumes of simulated oral fluid consisting of 4.0 mL of SSF, 25 μL of CaCl2(H2O)2 and 975 μL of deionized water, adjust the pH value of the mixture to 7.0 with HCl / NaOH solution, place the sample in a full-temperature oscillator, and digest it at 37°C for 2 min.

[0215] ② Simulated gastric digestion: The oral digestive fluid (10 mL) obtained in step ① was mixed with an equal volume of simulated gastric fluid containing 7.5 mL SGF, 5 μL CaCl(H2O)2, 0.2 mL HCl, and 695 μL deionized water, followed by the addition of 1.6 mL pepsin. The pH value of the mixture was adjusted to 3.0 with HCl solution, and the sample was placed in a full-temperature oscillator for digestion at 37°C for 2 h.

[0216] ③Simulated intestinal digestion: The gastric digestive fluid (20 mL) obtained in step ② was mixed with an equal volume of simulated intestinal fluid consisting of 11 mL SIF, 40 μL CaCl2(H2O)2, 0.15 mL NaOH and 1.31 mL deionized water, and 5 mL trypsin and 2.5 mL chymosin were added. The pH value of the mixed solution was adjusted to 7.0 with HCl / NaOH solution, and the sample was placed in a full-temperature oscillator and digested at 37°C for 2 h. After the sample was taken out of the full-temperature oscillator, the digestive fluid was immediately inactivated for 30 min, and then the inactivated digestive fluid was centrifuged (10000×g, 20 min), and the supernatant was refrigerated for later use. The protein content was determined by the GB 5009.5 2016 Kjeldahl method.

[0217]

[0218]

[0219]

[0220] It can be seen from the above data that the rice and pea plant dual protein prepared by the present invention can significantly improve the taste and particle size, and has a smooth and delicate taste without obvious beany smell. At the same time, the processing characteristics such as high solubility, emulsification and emulsification stability, foaming and foaming stability, and water retention are effectively improved, and the digestibility is high and the absorption is good, and the application in different products can be expanded.

[0221] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing rice and pea plant double protein, characterized in that: The following steps are involved: Rice protein is used as a raw material, water is added to prepare a rice protein raw material slurry, the pH value is adjusted, homogenized, high-temperature shearing is performed, and then pea protein powder is added to prepare a plant double protein slurry containing pea protein powder, the pH value is adjusted, homogenized, high-temperature shearing is performed, and then spray drying is performed to obtain rice and pea plant double protein; The pH value of the rice protein raw material slurry is adjusted to 7.5-11.5; The pH value of the plant double protein slurry is adjusted to 7.0-11.5; The dry basis mass ratio of the pea protein powder to the rice protein is 6:(2-4); The shearing equipment used in the high-temperature shearing includes a silo and an evaporator, the outlet of the silo is connected to the evaporator, a rotating drum is arranged inside the silo, and an annular channel is formed between the inner wall of the silo and the outer wall of the rotating drum; a feed pipe is arranged on the side wall of the silo, the discharge port of the feed pipe is connected to the annular channel, and the discharge direction is tangent to the side wall of the rotating drum; A steam pipeline is arranged on the top of the rotating drum, and the air outlet of the steam pipeline is connected with the interior of the rotating drum; a through hole is opened on the side wall of the rotating drum; the material passes through the feed pipe and quickly enters the annular channel along the tangent direction of the side wall of the rotating drum, and the rotating drum starts to rotate rapidly under the thrust of the material, and the material forms a thin film layer on the inner wall of the bin body. At the same time, the saturated steam with pressure passes through the through hole from the inside of the rotating drum and quickly contacts the material, and the material quickly heats up and then quickly extends, and the dispersed steam shears the material in an orderly manner, and the sheared material enters the evaporator to remove part of the water, and the evaporator plays a role in concentrating the sheared liquid; The high temperature shearing time of the rice protein raw material slurry is 30-60s; The flow rate of the high temperature shearing of the rice protein raw material slurry is 1-3T / h; The high temperature shearing time of the plant double protein slurry is set to 30-60s; The high temperature shearing flow rate of the plant double protein slurry is 1-3 T / h.

2. The preparation method according to claim 1, characterized in that: The solid content of the rice protein raw material slurry is 6-20%; And / or, the solid content of the plant double protein slurry is 6-20%.

3. The preparation method according to claim 1, characterized in that: The dry basis protein content of the rice protein is 80-85%; And / or, the dry basis protein content of the pea protein powder is greater than 80%.

4. The preparation method according to claim 1, characterized in that: The homogenization pressure of the rice protein raw material slurry is 30-55MPa; And / or, the rice protein raw material slurry is homogenized 1 to 2 times.

5. The preparation method according to claim 1, characterized in that: The temperature of the high temperature shearing of the rice protein raw material slurry is set to 130-170°C.

6. The preparation method according to claim 1, characterized in that: The plant double protein slurry The homogenization pressure of the material is 30-55MPa; And / or, the plant double protein slurry is homogenized 1 to 3 times.

7. The preparation method according to claim 1, characterized in that: The temperature of the high temperature shearing of the plant double protein slurry is set to 130-170°C.

8. The preparation method according to claim 1, characterized in that: The spray drying inlet temperature is 135-185°C, and the outlet temperature is 55-85°C.

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