A feed additive for relieving constipation of sows and a preparation method thereof

Abstract

The application discloses a feed additive for relieving constipation of sows and a preparation method thereof, and comprises the following steps: preparation of enzyme-decomposed primary wet powder, preparation of enzyme-decomposed powder fermentation material, preparation of polysaccharide compound iron-selenium liquid, preparation of polysaccharide compound iron-selenium fermentation material, preparation of acidified compound iron-selenium fermentation material and preparation of the feed additive. The application increases the complexation of iron-selenium and polysaccharide through a new traditional Chinese medicine prescription and acidified fermentation treatment of iron-selenium and polysaccharide, so that the produced feed additive can improve the activity of the sow body, promote the intestinal health, increase the intestinal peristalsis and greatly relieve the constipation of the sow.

Description

Technical Field

[0001] This invention relates to the field of feed additive preparation technology, specifically to a feed additive for relieving constipation in sows and its preparation method. Background Technology

[0002] Animal husbandry has a long history, and through selective breeding and propagation, pigs have become the main domesticated poultry. China is a major pig-producing country with huge economies of scale.

[0003] Pigs come in many varieties and have diverse uses. Meat pigs are the world's largest source of edible meat; pork is delicious, can be processed into many varieties, and is suitable for human consumption, making it popular with consumers. The productivity of sows is a crucial aspect of pig farming efficiency. Intestinal health of sows is an important factor in improving their profitability. A trend in intestinal health is towards green, efficient, and safe intestinal care for sows.

[0004] Constipation in sows has always been a persistent problem in pig farming. It not only affects the sow's nutrient absorption but also impacts litter size, average litter weight, and the number of healthy piglets. Therefore, alleviating sow constipation is a key aspect of improving sow farming efficiency. Using natural plant-based feed additives offers advantages such as multiple target points, significant effects, no toxic side effects, and safety. These plant-based additives can also provide good intestinal health benefits for pigs.

[0005] Chinese patent document CN202211627180.7 discloses a traditional Chinese medicine composition for preventing and treating constipation in pregnant sows and its preparation method. The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 25-30 parts of Scrophularia ningpoensis, 21-25 parts of Ophiopogon japonicus, 20-25 parts of Rehmannia glutinosa, 10-15 parts of Artemisia annua, 6-8 parts of Artemisia argyi, and 6-8 parts of Perilla frutescens. Although the prepared traditional Chinese medicine composition can improve the symptoms of constipation in sows to a certain extent, the herbal ingredients are only extracted through simple soaking and the efficacy of traditional Chinese medicine is not fully utilized, and the prevention and treatment effect still needs to be further improved. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide a feed additive for relieving constipation in sows and a method for preparing the same.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0009] S1. Preparation of enzymatically hydrolyzed primary wet powder: Add rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract to water and mix evenly to obtain primary wet powder. Then add hemicellulase, cellulase and pectinase to the primary wet powder to carry out enzymatic hydrolysis reaction to obtain enzymatically hydrolyzed primary wet powder.

[0010] S2. Preparation of enzymatic hydrolysate fermentation material: Add the brewer's yeast liquid, Clostridium butyricum liquid and Lactobacillus plantarum liquid to the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, seal and ferment to obtain enzymatic hydrolysate fermentation material.

[0011] S3. Preparation of polysaccharide composite iron-selenium solution: Peach gum, Poria cocos and Solanum nigrum are mixed evenly to obtain a mixed powder. Then, ferrous fumarate, nano selenium, cellulase, hemicellulase and water are added to it and mixed evenly. The mixture is kept at 45-60℃ for 6-10 hours to obtain polysaccharide composite iron-selenium solution.

[0012] S4. Preparation of polysaccharide-compound iron-selenium fermentation material: Add glucose and Clostridium butyricum bacterial solution to polysaccharide-compound iron-selenium solution, stir evenly, seal and ferment to obtain polysaccharide-compound iron-selenium fermentation material.

[0013] S5. Preparation of acidified composite iron-selenium fermentation material: Citric acid and water are added to the polysaccharide composite iron-selenium fermentation material and kept at 55-65℃ for 0.5-2h. Then lactic acid powder is added and kept at 55-65℃ for 2-4h. After drying, acidified composite iron-selenium fermentation material is obtained.

[0014] S6. Preparation of feed additives: Mix the enzymatically hydrolyzed fermented feed, acidified compound iron-selenium fermented feed and water evenly, and ferment to obtain feed additives.

[0015] Preferably, in step S1, the mass ratio of rice flower glutinous rice, ground ivy leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water is 4-6:5-8:3-7:1-3:8-15:0.8-2:10-15.

[0016] Preferably, in step S1, the amount of hemicellulase added is 5% of the mass of the primary wet powder, the amount of cellulase added is 5% of the mass of the primary wet powder, and the amount of pectinase added is 3% of the mass of the primary wet powder;

[0017] The temperature for the enzymatic hydrolysis reaction is 38-42℃, and the reaction time is 1-3 hours.

[0018] Preferably, in step S2, the amount of Saccharomyces cerevisiae liquid added is 1% (v / w) of the enzymatically hydrolyzed primary wet powder, the amount of Clostridium butyricum liquid added is 1% (v / w) of the enzymatically hydrolyzed primary wet powder, and the amount of Lactobacillus plantarum liquid added is 1% (v / w) of the enzymatically hydrolyzed primary wet powder.

[0019] Preferably, in step S2, the temperature for sealed fermentation is 30-33℃, and the time for sealed fermentation is 72-144h.

[0020] Preferably, in step S3, the mass ratio of peach gum, Poria cocos, and Solanum nigrum is 15-20:8-12:2-5; and the mass ratio of ferrous fumarate, nano-selenium, cellulase, hemicellulase, water, and mixed powder is 0.02:0.003:6:8:250:100.

[0021] Preferably, in step S4, the amount of glucose added is 1% (w / w) of the polysaccharide complex iron-selenium solution, and the amount of Clostridium butyricum solution added is 1% (v / w) of the polysaccharide complex iron-selenium solution.

[0022] The temperature for sealed fermentation is 30-33℃, and the fermentation time is 72-144 hours.

[0023] Preferably, in step S5, the mass ratio of citric acid, water, polysaccharide compound iron-selenium fermentation material, and lactic acid powder is 8-15:200-300:100:15-20.

[0024] Preferably, in step S6, the mass ratio of enzymatic hydrolysate fermentation material, acidified compound iron-selenium fermentation material, and water is 6-8:4-6:2-4.

[0025] The fermentation temperature is 30-33℃, and the sealed fermentation time is 24-48 hours.

[0026] The present invention also provides a feed additive for relieving constipation in sows prepared by the above preparation method.

[0027] Compared with the prior art, the present invention has the following beneficial effects: The present invention increases the complexation effect of iron selenium and polysaccharides through a new traditional Chinese medicine formula and the treatment of iron selenium and polysaccharide acidification and fermentation. The feed additive produced can improve the vitality of sows, promote intestinal health, increase intestinal peristalsis, and greatly relieve constipation in sows. Detailed Implementation

[0028] The present invention will be further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0029] It should be noted that, unless otherwise specified, all chemical reagents involved in this invention were purchased through commercial channels.

[0030] Clostridium butyricum was purchased from Qingdao Blue Ocean Biotechnology Co., Ltd.

[0031] The brewing yeast was purchased from Qingdao Weilan Biotechnology Co., Ltd.

[0032] Lactobacillus plantarum was purchased from Qingdao Weilan Biotechnology Co., Ltd.

[0033] Hemicellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 50,000 U / g;

[0034] Cellulase was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., at a concentration of 11000 U / g;

[0035] Pectinase (H type / viscosity reducer) was purchased from Ningxia Xiasheng Industrial Group Co., Ltd., 25000 U / g;

[0036] Black nightshade was purchased from Xi'an Guohao Biotechnology Co., Ltd.

[0037] The Elsholtzia ciliata was purchased from Nanjing Tongrentang Pharmaceutical Co., Ltd.

[0038] The leaves of *Georgium chinense* were purchased from Xi'an Qiancao Biotechnology Co., Ltd.

[0039] The blood-nourishing vegetables were purchased from Zhuoma Ama's flagship store;

[0040] Poria cocos was purchased from Xingyuan Grain and Oil Store in Luozhuang District;

[0041] The peach gum was purchased from Heyuntang flagship store;

[0042] The peptone was purchased from Jinan Lanjue Trading Co., Ltd.

[0043] The glucose was purchased from Shandong Xujie Chemical Co., Ltd.

[0044] Ferrous fumarate was purchased from Nanning Zewell Feed Co., Ltd.

[0045] Nano-selenium was purchased from Chengdu Chelating Biotechnology Co., Ltd.

[0046] Citric acid was purchased from Shanghai Mingjiong Biotechnology Co., Ltd.

[0047] Lactic acid powder was purchased from Qingdao Huazhiyuan Food Additives Co., Ltd.

[0048] The activation process of the bacterial solution in the various embodiments and comparative examples of the present invention is as follows:

[0049] Clostridium butyricum culture preserved on slant culture was scraped with an inoculation loop (loop diameter 0.3 mm), inoculated into 300 mL of activation medium, placed in a 500 mL Erlenmeyer flask, and cultured at 33 °C with a rotation speed of 180 r / m for 20 h to obtain Clostridium butyricum culture.

[0050] Saccharomyces cerevisiae culture preserved on slant surface was scraped with an inoculation loop (0.3 mm in diameter) to obtain hexacyclophosphamide, which was then inoculated into 300 mL of activation medium and placed in a 500 mL Erlenmeyer flask. The culture was carried out at 33 °C and 180 r / m for 20 h to obtain Saccharomyces cerevisiae culture solution.

[0051] The *Lactobacillus plantarum* strain preserved on slant culture was scraped with an inoculation loop (0.3 mm in diameter), inoculated into 300 mL of activation medium, placed in a 500 mL Erlenmeyer flask, and cultured at 33 °C with a rotation speed of 180 r / m for 22 h to obtain *Lactobacillus plantarum* bacterial suspension.

[0052] The culture medium consists of 1% (w / w) yeast extract, 1% (w / w) peptone, and 4% (w / w) glucose. After preparation, sterilize at 115°C for 30 minutes and cool to room temperature.

[0053] Black nightshade, fragrant rice glutinous rice, ground ivy leaves, poria cocos, peach gum, and blood-nourishing vegetables: Grind them separately using a grinder, and then pass them through an 80-mesh sieve.

[0054] Example 1

[0055] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0056] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 5:7:6:2:10:1:15. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder. The mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0057] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0058] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0059] S4. Preparation of polysaccharide-complex iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum bacterial solution to 100g polysaccharide-complex iron-selenium solution, stir evenly, put into a plastic bag, and seal and ferment at 32℃ for 96 h to obtain polysaccharide-complex iron-selenium fermentation material.

[0060] S5. Preparation of acidified composite iron-selenium fermentation material: 10g of citric acid monohydrate and 250g of water were added to 100g of polysaccharide composite iron-selenium fermentation material and kept at 60℃ for 1h. Then, 18g of lactic acid powder was added and kept at 60℃ for 3h. Then, the mixture was dried in a drying oven at 60℃ until the moisture content was 17% (w / w) to obtain acidified composite iron-selenium fermentation material.

[0061] S6. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 50g of acidified compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0062] Example 2

[0063] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0064] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 4:8:5:2:12:1.5:15. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder, and the mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0065] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0066] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0067] S4. Preparation of polysaccharide-complex iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum bacterial solution to 100g polysaccharide-complex iron-selenium solution, stir evenly, put into a plastic bag, and seal and ferment at 32℃ for 96 h to obtain polysaccharide-complex iron-selenium fermentation material.

[0068] S5. Preparation of acidified composite iron-selenium fermentation material: 10g of citric acid monohydrate and 250g of water were added to 100g of polysaccharide composite iron-selenium fermentation material and kept at 60℃ for 1h. Then, 18g of lactic acid powder was added and kept at 60℃ for 3h. Then, the mixture was dried in a drying oven at 60℃ until the moisture content was 17% (w / w) to obtain acidified composite iron-selenium fermentation material.

[0069] S6. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 50g of acidified compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0070] Example 3

[0071] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0072] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 6:5:4:2:9:1:10. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder. The mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0073] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0074] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0075] S4. Preparation of polysaccharide-complex iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum bacterial solution to 100g polysaccharide-complex iron-selenium solution, stir evenly, put into a plastic bag, and seal and ferment at 32℃ for 96 h to obtain polysaccharide-complex iron-selenium fermentation material.

[0076] S5. Preparation of acidified composite iron-selenium fermentation material: 10g of citric acid monohydrate and 250g of water were added to 100g of polysaccharide composite iron-selenium fermentation material and kept at 60℃ for 1h. Then, 18g of lactic acid powder was added and kept at 60℃ for 3h. Then, the mixture was dried in a drying oven at 60℃ until the moisture content was 17% (w / w) to obtain acidified composite iron-selenium fermentation material.

[0077] S6. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 50g of acidified compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0078] Example 4

[0079] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0080] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 5:8:4:1:15:2:15. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder, and the mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0081] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0082] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0083] S4. Preparation of polysaccharide-complex iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum bacterial solution to 100g polysaccharide-complex iron-selenium solution, stir evenly, put into a plastic bag, and seal and ferment at 32℃ for 96 h to obtain polysaccharide-complex iron-selenium fermentation material.

[0084] S5. Preparation of acidified composite iron-selenium fermentation material: 10g of citric acid monohydrate and 250g of water were added to 100g of polysaccharide composite iron-selenium fermentation material and kept at 60℃ for 1h. Then, 18g of lactic acid powder was added and kept at 60℃ for 3h. Then, the mixture was dried in a drying oven at 60℃ until the moisture content was 17% (w / w) to obtain acidified composite iron-selenium fermentation material.

[0085] S6. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 50g of acidified compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0086] Comparative Example 1

[0087] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0088] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 5:7:6:2:10:1:15. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder. The mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0089] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0090] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0091] S4. Preparation of polysaccharide-complex iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum bacterial solution to 100g polysaccharide-complex iron-selenium solution, stir evenly, put into a plastic bag, and seal and ferment at 32℃ for 96 h to obtain polysaccharide-complex iron-selenium fermentation material.

[0092] S5. Preparation of acidified composite iron-selenium fermentation material: 10g of citric acid monohydrate and 250g of water were added to 100g of polysaccharide composite iron-selenium fermentation material and kept at 60℃ for 1h. Then, 18g of lactic acid powder was added and kept at 60℃ for 3h. Then, the mixture was dried in a drying oven at 60℃ until the moisture content was 17% (w / w) to obtain acidified composite iron-selenium fermentation material.

[0093] S6. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 10g of acidified compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0094] Compared with Example 1, the proportions of each raw material in step S6 are different.

[0095] Comparative Example 2

[0096] A method for preparing a feed additive to relieve constipation in sows includes the following steps:

[0097] S1. Preparation of enzymatically hydrolyzed primary wet powder: Rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract were added to water, wherein the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water was 5:7:6:2:10:1:15. The mixture was stirred evenly to obtain primary wet powder. Based on the mass of primary wet powder, 5% hemicellulase, 5% cellulase and 3% pectinase were added to the primary wet powder. The mixture was kept at 40℃ for 2.0h to obtain enzymatically hydrolyzed primary wet powder.

[0098] S2. Preparation of enzymatic hydrolysate fermentation material: Add 1 mL of Saccharomyces cerevisiae liquid, 1 mL of Clostridium butyricum liquid and 1 mL of Lactobacillus plantarum liquid to 100 g of the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h to obtain enzymatic hydrolysate fermentation material.

[0099] S3. Preparation of polysaccharide composite iron-selenium solution: Mix 90g of peach gum, 50g of Poria cocos and 15g of Solanum nigrum evenly to obtain a mixed powder; weigh 100g of the mixed powder, and then add 0.02g of ferrous fumarate, 0.003g of nano selenium, 6g of cellulase, 8g of hemicellulase and 250g of water to it, mix evenly, and keep warm at 50℃ for 8h to obtain polysaccharide composite iron-selenium solution;

[0100] S4. Preparation of polysaccharide compound iron-selenium fermentation material: Add 1g glucose and 1mL Clostridium butyricum inoculum to 100g polysaccharide compound iron-selenium inoculum, stir evenly, put into a plastic bag, seal and ferment at 32℃ for 96 h, and then dry in a drying oven at 60℃ until the moisture content is 17% (w / w) to obtain polysaccharide compound iron-selenium fermentation material.

[0101] S5. Preparation of feed additives: Mix 70g of enzymatically hydrolyzed fermented feed, 50g of polysaccharide compound iron-selenium fermented feed and 30g of water evenly, and ferment at 32℃ for 36 hours to obtain feed additives.

[0102] Compared with Example 1, the polysaccharide-compound iron-selenium fermentation material does not undergo acidification treatment.

[0103] Comparative Example 3

[0104] Compared to Example 1, no peach gum was added.

[0105] Comparative Example 4

[0106] Compared to Example 1, no black nightshade was added.

[0107] Comparative Example 5

[0108] Compared to Example 1, no fragrant glutinous rice with pine nuts was added.

[0109] Comparative Example 6

[0110] Compared to Example 1, no earth-structure leaves were added.

[0111] Comparative Example 7

[0112] Compared to Example 1, no blood-nourishing vegetables were added.

[0113] The feed additives prepared in Example 1 and Comparative Examples 1-7 were tested to study their effects on constipation in sows. The specific experimental methods are as follows:

[0114] Nine hundred Duroc, Landrace, and Greenland sows of similar health and parity, aged 60 days of gestation, were randomly divided into nine groups, with ten replicates per group and ten sows per group. The control group was fed a basal diet, while the experimental groups were fed a basal diet plus 2% feed additive (i.e., the feed additives prepared in Example 1 and Comparative Examples 1-7). The experimental period was 13 days. The basal diet composition was corn:soybean meal:concentrate (Baiyiyun Pig Concentrate, Hunan Baiyiyun Business Technology Co., Ltd.) at a ratio of 64:24:12. Statistical criteria were as follows: Severe constipation: difficulty in defecation, feces were granular and hard; Mild constipation: feces were cylindrical or contained some granules, and were relatively hard when stepped on; Moderate constipation: easy defecation, soft feces that crumbled upon impact. The number of sows was calculated as the average over 13 days, calculated as the sum of the number of sows with constipation per day / 13. The experimental results are shown in Table 1.

[0115] Table 1

[0116]

[0117] Finally, it should be noted that the above embodiments do not limit the present invention in any way. Those skilled in the art can make modifications and improvements based on the present invention. Therefore, any modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A method for preparing a feed additive to relieve constipation in sows, characterized in that, Includes the following steps: S1. Preparation of enzymatically hydrolyzed primary wet powder: Add rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, and loofah extract to water and mix evenly to obtain primary wet powder. Then add hemicellulase, cellulase and pectinase to the primary wet powder to carry out enzymatic hydrolysis reaction to obtain enzymatically hydrolyzed primary wet powder. S2. Preparation of enzymatic hydrolysate fermentation material: Add the brewer's yeast liquid, Clostridium butyricum liquid and Lactobacillus plantarum liquid to the enzymatic hydrolysate primary wet powder obtained in step S1, stir evenly, seal and ferment to obtain enzymatic hydrolysate fermentation material. S3. Preparation of polysaccharide composite iron-selenium solution: Peach gum, Poria cocos and Solanum nigrum are mixed evenly to obtain a mixed powder. Then, ferrous fumarate, nano selenium, cellulase, hemicellulase and water are added to it and mixed evenly. The mixture is kept at 45-60℃ for 6-10 hours to obtain polysaccharide composite iron-selenium solution. S4. Preparation of polysaccharide-compound iron-selenium fermentation material: Add glucose and Clostridium butyricum bacterial solution to polysaccharide-compound iron-selenium solution, stir evenly, seal and ferment to obtain polysaccharide-compound iron-selenium fermentation material. S5. Preparation of acidified composite iron-selenium fermentation material: Citric acid and water are added to the polysaccharide composite iron-selenium fermentation material and kept at 55-65℃ for 0.5-2h. Then lactic acid powder is added and kept at 55-65℃ for 2-4h. After drying, acidified composite iron-selenium fermentation material is obtained. S6. Preparation of feed additives: Mix the enzymatically hydrolyzed fermented feed, acidified compound iron-selenium fermented feed and water evenly, and ferment to obtain feed additives. In step S1, the mass ratio of rice flower glutinous rice, ground mulberry leaf, blood-nourishing vegetable, winter melon powder, five-clawed peach, loofah extract and water is 4-6:5-8:3-7:1-3:8-15:0.8-2:10-15.

2. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S1, based on the mass of the primary wet powder, the amount of hemicellulase added is 5%, the amount of cellulase added is 5%, and the amount of pectinase added is 3%. The temperature for the enzymatic hydrolysis reaction is 38-42℃, and the reaction time is 1-3 hours.

3. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S2, based on the mass of the primary wet powder from enzymatic hydrolysis, the amount of added Saccharomyces cerevisiae culture solution is 1% v / w, the amount of added Clostridium butyricum culture solution is 1% v / w, and the amount of added Lactobacillus plantarum culture solution is 1% v / w.

4. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S2, the temperature for sealed fermentation is 30-33℃, and the fermentation time is 72-144h.

5. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S3, the mass ratio of peach gum, Poria cocos, and Solanum nigrum is 15-20:8-12:2-5; the mass ratio of ferrous fumarate, nano selenium, cellulase, hemicellulase, water, and mixed powder is 0.02:0.003:6:8:250:

100.

6. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S4, based on the mass of the polysaccharide complex iron-selenium solution, the amount of glucose added is 1% w / w, and the amount of Clostridium butyricum solution added is 1% v / w. The temperature for sealed fermentation is 30-33℃, and the fermentation time is 72-144 hours.

7. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S5, the mass ratio of citric acid, water, polysaccharide compound iron-selenium fermentation material, and lactic acid powder is 8-15:200-300:100:15-20.

8. The method for preparing the feed additive for relieving constipation in sows according to claim 1, characterized in that, In step S6, the mass ratio of enzymatic hydrolysate fermentation material, acidified compound iron-selenium fermentation material and water is 6-8:4-6:2-4. The fermentation temperature is 30-33℃, and the sealed fermentation time is 24-48 hours.

9. The feed additive for relieving constipation in sows prepared by the preparation method according to any one of claims 1-8.

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