An active polypeptide nutrient and its preparation process

Abstract

The application provides an active polypeptide nutrient and a preparation process thereof, and comprises the following raw materials in parts by weight: coconut milk powder 40-90 parts, fish collagen peptide 29-53 parts, sea cucumber peptide 25-35 parts, oyster peptide 18-29 parts, has rich coconut fragrance, no fishy smell, is milky white, has fresh taste, mellow taste, and no stimulation; the prepared product can improve immunity, oxidation resistance and fatigue resistance, and effectively help the human body to repair damaged cells, improve skin elasticity and glossiness; the coconut milk polypeptide is obtained through enzymolysis technology, is mixed with other polypeptides, improves the nutritional value and health care function of the product; the polypeptide is adjusted in conductivity, then is conditioned, can strengthen the mixing of the coconut milk and the polypeptides, reduces the damage of high temperature, low temperature and centrifugation to the polypeptides, maintains the structure and functional integrity of the polypeptides, effectively increases the solubility and biological activity of the polypeptides; the embedding technology is used to isolate the external force, so as to protect the polypeptides from damage and degradation in the gastrointestinal tract, and improve the bioavailability and stability of the polypeptides.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food technology processing, in particular to an active polypeptide nutrient and a preparation process thereof. BACKGROUND

[0002] Peptides are important substances related to the functions of various cells in the human body. Peptides can synthesize cells and regulate the functional activities of cells. Peptides act as neurotransmitters in the human body, transmitting information. Peptides can act as transport tools in the human body, transporting various nutrients, vitamins, biotin, calcium and trace elements beneficial to the human body to cells, organs and tissues in the human body. Peptides are important physiological regulators of the human body, which can comprehensively regulate physiological functions of the human body, enhance and exert physiological activities of the human body, and have important biological functions. Peptides are irreplaceable for the activity, functional activity and life of human cells.

[0003] Coconut milk contains rich nutrients such as sugars, fats, proteins, vitamins and minerals, and also contains a variety of bioactive substances, among which polypeptides are one of them. Coconut milk polypeptides have various biological activities such as antioxidant, anti-inflammatory and antibacterial effects. These polypeptide substances have low content in coconut milk, but have important physiological functions.

[0004] Fish collagen peptide is a protein extracted from fish scales, fish skin, fish bones and other fish waste, which is a natural collagen peptide with good biocompatibility and biological activity. This protein is composed of various amino acids, including glycine, serine, lysine, proline, etc. Compared with other sources of collagen on the market, fish collagen peptide has a smaller molecular weight, is easy to absorb and utilize by the human body, and does not contain cholesterol and fat and other undesirable ingredients, so it has been widely welcomed. Fish collagen peptide is mainly used in beauty care and health food, and has the effects of promoting skin elasticity, moisturizing, delaying skin aging, promoting bone and joint health, etc. Its preparation process is relatively complex, and the raw materials are relatively scarce, especially the pollution-free deep-sea fish, which has a high cost. Choosing collagen without additives and pollution is a safe choice.

[0005] Sea cucumber contains bioactive calcium, sea cucumber mucopolysaccharide, polypeptide, sea cucumber element, sea cucumber saponin, amino acid and other active ingredients, which can effectively repair damaged joint cartilage and restore the normal function of bone joints. At the same time, the nutritional ingredients in sea cucumber also have the functions of expanding blood vessels, increasing blood flow, inhibiting platelet aggregation, enhancing fibrinolysis, improving microcirculation, promoting inflammation exudate absorption, analgesia, sedation, anti-inflammatory, anti-infection and improving the body's immunity. Methionine, taurine, trace elements vanadium, selenium, germanium and vitamin PP are indispensable substances for human growth and development, which play an irreplaceable physiological function in the body, such as methionine and taurine, which can promote growth and development and relieve fatigue; trace element vanadium can make the body more effectively absorb iron elements and improve various anemias; elements selenium and germanium have the functions of removing free radicals, preventing and resisting cancer.

[0006] Oyster peptide is a kind of oyster peptide prepared by using peptide molecular biological technology in the oyster processing process. The small molecular oligopeptide formed by this preparation method completely retains the original vitamins, trace elements and taurine and other nutrients of oysters, so that the oysters rich in nucleic acid are more easily absorbed by the human body after being taken into the human body, and have more important biological activity in human metabolism.

[0007] The above-mentioned polypeptides each have different advantages, and mixed use can take advantage of each other and play a better health care role. However, the physical and chemical properties of the polypeptides are slightly different, the polypeptide powder is a high molecular weight substance, and when mixed, the molecular weight difference may cause precipitation, resulting in product stratification or precipitation. Fish collagen peptide, sea cucumber peptide and oyster peptide are extracted from marine organisms, and marine organisms contain rich amino acids, proteins and other natural ingredients. These substances are constantly metabolized, oxidized and interacted, so that sulfur-containing and nitrogen-containing compounds are transformed, free fatty acids are rancid, and it is difficult to remove the fishy smell. It is difficult to achieve the ideal deodorizing effect by adding a single masking agent. SUMMARY

[0008] In view of this, the present application provides an active polypeptide nutrient and a preparation process thereof, which solve the above problems.

[0009] The technical scheme of the present application is as follows: an active polypeptide nutrient: including the following raw materials by weight: 40-90 parts of coconut milk powder, 29-53 parts of fish collagen peptide, 25-35 parts of sea cucumber peptide and 18-29 parts of oyster peptide.

[0010] Further, an active polypeptide nutrient: including the following raw materials by weight: 65 parts of coconut milk powder, 41 parts of fish collagen peptide, 30 parts of sea cucumber peptide and 23 parts of oyster peptide.

[0011] Further, a preparation process of an active polypeptide nutrient includes the following steps:

[0012] Step S1, preparing coconut milk powder: fresh coconut milk is selected as raw material, enzyme preparation is added for enzymatic reaction, the obtained enzymatic solution is separated and purified, concentrated, dried to obtain coconut milk powder;

[0013] Step S2, removing fishy smell: fish collagen peptide, sea cucumber peptide and oyster peptide are mixed and dissolved in a solvent to obtain a polypeptide solution, an adsorbent is added, a low-voltage pulse electric field is applied, magnetic stirring is carried out at a speed of 2000-4000 rpm for 25-55 min, the adsorbent is separated out, the solution is freeze-dried, and a mixed polypeptide after removing fishy smell is obtained;

[0014] Step S3, adjusting quality and mixing: the coconut milk powder and the mixed polypeptide are mixed, the conductivity is adjusted to 20-40 μS / cm, the mixture is put into a quality adjuster, saturated water vapor is introduced, and the quality adjustment is stopped when the moisture content is increased to 40-58%, a mixed polypeptide solution is obtained, an ultrafiltration membrane with a molecular cut-off of 1 kDa is used for screening, and a mixed polypeptide solution with a molecular weight of less than 1 kDa is obtained;

[0015] Step S4, embedding: the mixed polypeptide solution and the nanoparticle material are ultrasonically mixed at a volume ratio of 15-24:12 for 20-40 min, dried, and the excess solution is removed, dried, and an active polypeptide nutrient is obtained.

[0016] Further, the method for preparing the coconut milk powder in step S1 includes the following steps:

[0017] S11, preparing raw material: fresh coconut milk is selected as raw material, filtered and impurity-removed for standby;

[0018] S12, enzymolysis: the coconut milk is placed at 28-38℃ and pH 5-7, enzyme preparation is added for enzymatic reaction for 12-24 h to obtain a mixed solution, and the enzyme preparation is acid carboxyl peptidase and proline peptidase with a mass ratio of 2-5:8;

[0019] S13, separation and purification: the mixed solution is separated, filtered and centrifuged to remove residues and impurities, and a clear enzymatic solution is obtained;

[0020] S14, concentration: the obtained enzymatic solution is concentrated, the excess water is removed, and spray drying or vacuum drying is carried out to obtain coconut milk powder.

[0021] Further, the adsorbent in step S2 is activated carbon or activated clay with a particle size of 0.3-0.5 mm.

[0022] Further, the solvent in step S2 is water or a dilute acid solution with a concentration less than 1 mol / L, or a dilute alkali solution, wherein the dilute acid solution is selected from dilute hydrochloric acid and dilute nitric acid, and the dilute alkali solution is sodium hydroxide, potassium hydroxide or calcium hydroxide.

[0023] Further, the voltage of the low-voltage pulsed electric field in step S2 is 50-80 V, and the frequency is 40-50 Hz.

[0024] Further, the temperature in step S3 is 60-80 DEG C.

[0025] Further, the ultrasonic power in step S4 is 300-800 W, and the ultrasonic frequency is 250-350 kHz.

[0026] Further, the nanoparticle material in step S4 is chitosan nanoparticles or liposome nanoparticles.

[0027] Compared with the prior art, the present application has the following advantages:

[0028] The active polypeptide nutrient of the present application has a rich coconut fragrance, no fishy smell, a milky white color, a fresh taste, a mellow taste, and no irritation. The polypeptide component in coconut milk polypeptide can regulate human immunity, promote mineral absorption, and has antioxidant effects. By decomposing the protein in coconut juice into polypeptides through enzymatic technology, and mixing coconut milk polypeptide and various polypeptides together, the advantages of the two can be fully utilized, and the nutritional value and health care function of the product can be improved. The polypeptide is adjusted in conductivity and then conditioned, so as to reduce the damage of high temperature to the polypeptide, maintain the structural and functional integrity of the polypeptide, reduce the crystallinity and aggregation degree of the polypeptide, increase the solubility and biological activity of the polypeptide, maintain good performance of the polypeptide under low temperature conditions, reduce the aggregation degree and sedimentation tendency of the polypeptide, reduce the damage of centrifugation to the polypeptide, and strengthen the mixing of coconut milk and various polypeptides, increase the chemical stability and physical stability of the polypeptide. The embedding technology is used to isolate the polypeptide from external forces, so as to protect the polypeptide from damage and degradation in the gastrointestinal tract, improve the bioavailability and stability of the polypeptide, solve the separation or precipitation phenomenon of various polypeptides due to different physical and chemical properties when mixed, provide various nutritional components and biological active substances, help to improve immunity, antioxidant and anti-fatigue, effectively help the human body to repair damaged cells, and improve skin elasticity and glossiness. It is a preferred product for modern people to pursue healthy and beautiful skin. DETAILED DESCRIPTION

[0029] In order to better understand the technical content of the present application, the following specific examples are provided to further illustrate the present application.

[0030] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.

[0031] The materials, reagents and the like used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0032] Embodiment 1

[0033] An active polypeptide nutrient: including the following weight parts of raw materials: coconut milk powder 40 parts, fish collagen peptide 29 parts, sea cucumber peptide 25 parts, oyster peptide 18 parts.

[0034] Embodiment 2

[0035] An active polypeptide nutrient: including the following weight parts of raw materials: coconut milk powder 90 parts, fish collagen peptide 53 parts, sea cucumber peptide 35 parts, oyster peptide 29 parts.

[0036] Embodiment 3

[0037] An active polypeptide nutrient: including the following weight parts of raw materials: coconut milk powder 65 parts, fish collagen peptide 41 parts, sea cucumber peptide 30 parts, oyster peptide 23 parts;

[0038] The above embodiments 1-3 use the following preparation process:

[0039] Step S1, preparing coconut milk powder includes the following steps:

[0040] S11, preparing raw materials: selecting fresh coconut water as raw material, after filtering and impurity removal treatment, it is ready for use;

[0041] S12, enzymolysis: taking the above coconut water, adding enzyme preparation for enzymolysis reaction at 33℃ and pH 6 for 12-24h, obtaining a mixed solution, the enzyme preparation is acid carboxyl peptidase and proline peptidase with a mass ratio of 3:8;

[0042] S13, separation and purification: separating, filtering and centrifuging the above mixed solution to remove residues and impurities, obtaining a clear enzymolysis solution;

[0043] S14, concentration: concentrating the obtained enzymolysis solution, removing excess water, vacuum drying, obtaining coconut milk powder;

[0044] Step S2, deodorization: mixing fish collagen peptide, sea cucumber peptide and oyster peptide, dissolving in 0.3mol / L dilute hydrochloric acid to obtain a polypeptide solution, adding activated carbon with a particle size of 0.4mm, applying low-voltage pulsed electric field with a voltage of 70V and a frequency of 45Hz, magnetically stirring at a speed of 3000rpm for 40min, separating the adsorbent, freeze-drying the solution to obtain deodorized mixed polypeptide;

[0045] Step S3, conditioning mixing: the coconut milk powder and the mixed polypeptide are mixed, the conductivity is adjusted to 30 μS / cm, and the mixed polypeptide solution is obtained by mixing in a conditioner at 70°C, passing in saturated water vapor, and stopping conditioning when the moisture content is increased to 48%; the mixed polypeptide solution with a molecular weight of less than 1 kDa is obtained by screening with an ultrafiltration membrane with a molecular weight cut-off of 1 kDa;

[0046] Step S4, embedding: the mixed polypeptide solution and the chitosan nanoparticles are ultrasonically mixed at a volume ratio of 20:12 for 30 min at an ultrasonic power of 500 W and an ultrasonic frequency of 300 kHz, dried, and the excess solution is removed, and the active polypeptide nutrient is obtained by drying.

[0047] Example 4

[0048] An active polypeptide nutrient: including the following raw materials by weight: coconut milk powder 65 parts, fish collagen peptide 41 parts, sea cucumber peptide 30 parts, oyster peptide 23 parts;

[0049] The preparation process used in the above example 4 is as follows:

[0050] Step S1, preparing coconut milk powder, including the following steps:

[0051] S11, preparing raw materials: fresh coconut water is selected as the raw material, which is filtered and impurity-removed for standby;

[0052] S12, enzymatic hydrolysis: the coconut water is placed in an enzyme preparation at 28°C and pH 5 for enzymatic hydrolysis for 12 h to obtain a mixed solution, and the enzyme preparation is an acid carboxyl peptidase and proline peptidase with a mass ratio of 2:8;

[0053] S13, separation and purification: the mixed solution is separated, filtered, and centrifuged to remove residues and impurities, and a clear enzymatic hydrolysate is obtained;

[0054] S14, concentration: the obtained enzymatic hydrolysate is concentrated under reduced pressure, and the excess water is removed for vacuum drying to obtain coconut milk powder;

[0055] Step S2, deodorization: the fish collagen peptide, sea cucumber peptide, and oyster peptide are mixed and dissolved in 0.7 mol / L dilute nitric acid to obtain a polypeptide solution, activated carbon with a particle size of 0.3-0.5 mm is added, a low-voltage pulsed electric field with a voltage of 50 V and a frequency of 40 Hz is applied, magnetic stirring is performed at a speed of 2000 rpm for 25 min, the adsorbent is separated out, and the solution is freeze-dried to obtain deodorized mixed polypeptide;

[0056] Step S3, conditioning mixing: the coconut milk powder and the mixed polypeptide are mixed, the conductivity is adjusted to 20 μS / cm, and the mixed polypeptide solution is obtained by mixing in a conditioner at 60°C, passing in saturated water vapor, and stopping conditioning when the moisture content is increased to 40%. The mixed polypeptide solution with a molecular weight of less than 1 kDa is obtained by screening with an ultrafiltration membrane with a molecular weight cut-off of 1 kDa.

[0057] Step S4, embedding: the mixed polypeptide solution and the chitosan nanoparticles are ultrasonically mixed at a volume ratio of 15:12 for 20 min, the ultrasonic power is 300 W, the ultrasonic frequency is 250 kHz, and the active polypeptide nutrient is obtained by drying and removing excess solution.

[0058] Example 5

[0059] An active polypeptide nutrient: including the following raw materials by weight: coconut milk powder 65 parts, fish collagen peptide 41 parts, sea cucumber peptide 30 parts, oyster peptide 23 parts;

[0060] The preparation process of the above example 5 is as follows:

[0061] Step S1, preparing coconut milk powder, including the following steps:

[0062] S11, preparing raw materials: fresh coconut water is selected as the raw material, and is prepared by filtering and removing impurities;

[0063] S12, enzymatic hydrolysis: the coconut water is placed in an enzyme preparation at 38°C and pH 7 for 24 h to obtain a mixed solution, and the enzyme preparation is acid carboxyl peptidase and proline peptidase with a mass ratio of 5:8;

[0064] S13, separation and purification: the mixed solution is separated, filtered, and centrifuged to remove residues and impurities, and a clear enzymatic hydrolysate is obtained;

[0065] S14, concentration: the obtained enzymatic hydrolysate is concentrated, and the excess water is removed and vacuum dried to obtain coconut milk powder;

[0066] Step S2, deodorization: the fish collagen peptide, sea cucumber peptide, and oyster peptide are mixed and dissolved in 0.5 mol / L sodium hydroxide to obtain a polypeptide solution, active white clay with a particle size of 0.5 mm is added, a low-voltage pulsed electric field with a voltage of 80 V and a frequency of 50 Hz is applied, and the solution is magnetically stirred at a speed of 4000 rpm for 55 min. The adsorbent is separated, the solution is freeze-dried, and the deodorized mixed polypeptide is obtained.

[0067] Step S3, conditioning mixing: the coconut milk powder and the mixed polypeptide are mixed, the conductivity is adjusted to 40 μS / cm, and the mixed polypeptide solution is obtained by mixing in a conditioner at 80°C, stopping conditioning when the moisture content is increased to 58% by passing in saturated water vapor; the mixed polypeptide solution with a molecular weight of less than 1 kDa is obtained by screening with an ultrafiltration membrane with a molecular cut-off of 1 kDa;

[0068] Step S4, embedding: the mixed polypeptide solution and the liposome nanoparticles are ultrasonically mixed at a volume ratio of 24:12 for 40 min, the ultrasonic power is 800 W, the ultrasonic frequency is 350 kHz, and the active polypeptide nutrient is obtained by drying and removing excess solution.

[0069] Comparative Example 1

[0070] The difference between this comparative example and Example 3 is that the active polypeptide nutrient comprises the following raw materials by weight: coconut milk powder 35 parts, fish collagen peptide 63 parts, sea cucumber peptide 15 parts, and oyster peptide 15 parts.

[0071] Comparative Example 2

[0072] The difference between this comparative example and Example 3 is that the preparation process of the active polypeptide nutrient does not apply low-voltage pulsed electric field to assist in deodorization in the deodorization step of S2.

[0073] Comparative Example 3

[0074] The difference between this comparative example and Example 3 is that the preparation process of the active polypeptide nutrient does not apply low-voltage pulsed electric field to assist in deodorization in the deodorization step of S2.

[0075] Comparative Example 4

[0076] The difference between this comparative example and Example 3 is that the preparation process of the active polypeptide nutrient does not apply low-voltage pulsed electric field to assist in deodorization in the deodorization step of S2.

[0077] I. Performance Test

[0078] The solubility, stability, and biological activity of the active polypeptide nutrients obtained in Examples 1-5 and Comparative Examples 1-4 are determined, and the determination method is as follows:

[0079] 1. Solubility test: the solubility is verified by measuring the surface tension of the liquid film. The lower the surface tension, the better the dispersibility of the substance in the solvent, and the higher the solubility. Conversely, the higher the surface tension, the worse the dispersibility of the substance in the solvent, and the lower the solubility.

[0080] The surface tension test method is to slowly add the solution into the metal ring placed in the known surface tension meter, and observe the maximum height of the bubble. According to the formula: σ = 2γ / r, wherein σ is the surface tension, γ is the surface tension of the metal ring, and r is the radius of the metal ring, the surface tension of the solution can be calculated, and the test results are as follows:

[0081] Surface tension (dyn / cm) Example 1 20.3 Example 2 19.8 Example 3 18.4 Example 4 20.1 Example 5 21.4 Comparative Example 3 49.4 Comparative Example 4 40.3

[0082] By comparing the above results, the solubility of the coconut milk and the mixed polypeptide is improved by the tempering mixing of step S3; it is explained that the tempering can control the solubility of the polypeptide in the solution by adjusting the conductivity of the polypeptide; the embedding technology can change the solubility of the polypeptide, and improve the solubility of the polypeptide in the specific solvent,

[0083] 2. Stability test: the prepared polypeptide solution is respectively tested for heat stability test, cold stability test and centrifugal stability test, three parallel experiments for each experiment,

[0084] (1) Heat or cold stability test: heat stability test: heat the sample to 50℃ for 30min, cold stability test: place the sample at-15℃ for 45min, observe the change of physical properties, such as whether there is precipitation, color change and other phenomena;

[0085] (2) Centrifugal stability test: through 4000rpm centrifugal test for 20min, observe whether there is precipitation in the polypeptide solution, and measure the change of polypeptide content in the polypeptide solution before and after centrifugation;

[0086] (3) Data results:

[0087]

[0088] Note: “-” means no precipitation, “+” means precipitation, and “+” quantity means the amount of precipitation;

[0089] The above results show that the mixed conditioning can enhance the mixing of coconut milk and each polypeptide, increase the chemical stability and physical stability of the polypeptide, reduce the degradation and loss of the polypeptide during storage and transportation, and thus improve the stability thereof; compared with Comparative Example 3, the conditioning after adjusting the conductivity of the polypeptide can increase the stability of the polypeptide, reduce the damage of high temperature to the polypeptide, maintain the structural and functional integrity of the polypeptide, reduce the crystallinity and aggregation degree of the polypeptide, increase the solubility and biological activity of the polypeptide, and maintain good performance of the polypeptide under low temperature conditions; meanwhile, the conditioned polypeptide solution can reduce the aggregation degree and sedimentation tendency of the polypeptide, improve the centrifugal stability of the polypeptide, reduce the damage of centrifugation to the polypeptide, maintain the structural and functional integrity of the polypeptide, and improve the centrifugal separation and purification effect of the polypeptide; and the solubility and stability of the polypeptide in the solution can be effectively controlled;

[0090] Compared with Comparative Example 4, the embedding technology can protect the polypeptide from being damaged by high temperature, maintain the structural and functional integrity of the polypeptide, protect the polypeptide from crystallization and sedimentation caused by low temperature, maintain the stability of the structural and functional integrity of the polypeptide, and the polypeptide is wrapped in the protective layer and isolated from external forces, thereby reducing the occurrence of aggregation and sedimentation. Meanwhile, the embedding technology can further improve the centrifugal stability of the polypeptide nutrient by changing the solubility and aggregation state of the polypeptide nutrient.

[0091] 3. Biological activity

[0092] The biological activity of the polypeptide solution is tested by a cell experiment, and the following steps are followed:

[0093] 1) Preparation of cells: mouse fibroblasts (L929 cells) are selected for culture and amplification. Ensure that the cells are in the logarithmic growth phase for the experiment.

[0094] 2) Preparation of the solution to be tested: according to the experimental requirements, the polypeptide is dissolved in a suitable solvent to prepare a 1 mg / ml polypeptide nutrient solution. Ensure that the purity and concentration of the polypeptide meet the experimental requirements.

[0095] 3) Cell treatment: divide the cells into experimental and control groups, add the polypeptide nutrient solution to the experimental group, add the polypeptide nutrient solution of Comparative Example 3 to control group 1, add the polypeptide nutrient solution of Comparative Example 4 to control group 2, and add an equal amount of sterile water to the blank group.

[0096] 4) Cultivation of cells: the treated cells are cultured at 22°C and 95% humidity for 3 days to allow the polypeptide to function.

[0097] 5) Observation index: At appropriate time, observe the changes of related indexes such as cell morphology, proliferation, differentiation, apoptosis, etc. The morphology and growth of cells can be observed by microscope, the number and proliferation of cells can be determined by cell counting kit, and the cell cycle and apoptosis can be detected by flow cytometry, etc.

[0098] 6) Data analysis: Collect experimental data and perform statistical analysis. Compare the differences between experimental group and control group, and evaluate the influence of polypeptide on cell function.

[0099] 7) Repeat experiment: In order to ensure the reliability and stability of experimental results, repeat experiment is needed, and comprehensive evaluation is carried out by using multiple biological indexes.

[0100] Test results:

[0101] Proliferation rate % Apoptosis rate % Experimental group (Example 5) 27.4 21.3 Control group 1 (Comparative Example 3) 17.5 13.4 Control group 2 (Comparative Example 4) 19.5 15.6 Blank group 1.3 2.4

[0102] The results of observing the proliferation of cells show that, under the action of the active polypeptide nutrient solution of the application, the number of cells in the experimental group increases significantly, and the proliferation rate and apoptosis rate are significantly higher than those of the control groups 1 and 2, which indicates that the polypeptide nutrient solution has the activity of promoting cell proliferation,

[0103] II. Sensory evaluation

[0104] According to the general principles and methods of food sensory evaluation system, according to the related requirements of sensory characteristics, physical and chemical indexes and health indexes of polypeptide solution, 100 food professionals are selected to evaluate the active polypeptide nutrient solution from the three indexes of smell, color and taste through the sensory systems of olfaction, vision and taste, and the specific evaluation standards are as follows:

[0105]

[0106] The results of sensory evaluation are as follows:

[0107]

[0108]

[0109] Compared with the commercially available polypeptide solution, the active polypeptide nutrient of the application has rich coconut fragrance, no fishy smell, presents milky white color, fresh taste, mellow taste, and no irritation; it can be known from the smell of the comparative example 2 that the low-voltage pulsed electric field can change the ion concentration and charge distribution in the solution, and affect the properties of polypeptide molecules, thereby effectively removing the fishy substances in the polypeptide solution.

[0110] The above merely provides the preferred embodiment of the present application, and is not used to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A process for the preparation of an active polypeptide nutrient, characterized in that: The active polypeptide nutrient is prepared from 40-90 parts by weight of coconut milk powder, 29-53 parts of fish collagen peptide, 25-35 parts of sea cucumber peptide and 18-29 parts of oyster peptide. The preparation of the active polypeptide nutrient comprises the following steps: Step S1, preparing coconut milk powder: fresh coconut juice is selected as raw material, enzyme preparation is added for enzymolysis reaction, the obtained enzymolysis solution is separated and purified, concentrated, and dried to obtain coconut milk powder; Step S2, removing fishy smell: fish collagen peptide, sea cucumber peptide and oyster peptide are mixed and dissolved in a solvent to obtain a polypeptide solution, an adsorbent is added, a low-voltage pulsed electric field is applied, magnetic stirring is performed at a speed of 2000-4000 rpm for 25-55 min, the adsorbent is separated, the solution is freeze-dried, and a mixed polypeptide after removing fishy smell is obtained; Step S3, adjusting and mixing: the coconut milk powder and the mixed polypeptide are mixed, the conductivity is adjusted to 20-40 μS / cm, the mixture is placed in a conditioner, saturated water vapor is introduced, the conditioning is stopped when the moisture content is increased to 40-58%, a mixed polypeptide solution is obtained, an ultrafiltration membrane with a molecular cut-off of 1 kDa is used for screening, and a mixed polypeptide solution with a molecular weight of less than 1 kDa is obtained; Step S4, embedding: the mixed polypeptide solution and the nanoparticle material are ultrasonically mixed at a volume ratio of 15-24:12 for 20-40 min, dried, and the excess solution is removed, and dried to obtain the active polypeptide nutrient. The nanoparticle material in step S4 is chitosan nanoparticles or liposome nanoparticles.

2. A process for the preparation of an active polypeptide nutrient according to claim 1, characterized in that: The active polypeptide nutrient is prepared from 40-90 parts by weight of coconut milk powder, 29-53 parts of fish collagen peptide, 25-35 parts of sea cucumber peptide and 18-29 parts of oyster peptide.

3. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The method for preparing the coconut milk powder in step S1 comprises the following steps: S11, preparing raw materials: fresh coconut juice is selected as raw material, and is filtered and impurity-removed for standby; S12, enzymolysis: the coconut juice is placed in a 28-38℃ environment with pH of 5-7, and enzyme preparation is added for enzymolysis reaction for 12-24 h to obtain a mixed solution, wherein the enzyme preparation is acid carboxyl peptidase and proline peptidase with a mass ratio of 2-5:8; S13, separation and purification: the mixed solution is separated, filtered and centrifuged to remove residues and impurities, and a clear enzymolysis solution is obtained; S14, concentration: the obtained enzymolysis solution is concentrated to remove excess water, and is spray-dried or vacuum-dried to obtain coconut milk powder.

4. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The adsorbent in step S2 is activated carbon or activated clay with a particle size of 0.3-0.5 mm.

5. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The solvent in step S2 is water or a dilute acid solution with a concentration of less than 1 mol / L or a dilute alkali solution with a concentration of less than 1 mol / L, the dilute acid solution is selected from dilute hydrochloric acid and dilute nitric acid, and the dilute alkali solution is sodium hydroxide, potassium hydroxide or calcium hydroxide.

6. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The low-voltage pulsed electric field in step S2 has a voltage of 50-80 V and a frequency of 40-50 Hz.

7. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The conditioning temperature in step S3 is 60-80℃.

8. The preparation process of an active polypeptide nutrient as described in claim 1, characterized in that: The ultrasonic power in step S4 is 300-800 W, and the ultrasonic frequency is 250-350 kHz.

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