Apparatus and method for rapid treatment of tobacco leaf with a biological enzyme

The highly integrated bio-enzyme processing equipment has solved the problems of lack of standards and complexity in the tobacco processing process, realizing the automation, environmental consistency and speed of tobacco processing, and improving experimental efficiency and data acquisition capabilities.

CN117859946BActive Publication Date: 2026-02-03ZHENGZHOU TOBACCO RES INST OF CNTC +1
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Patent Information

Application Number
CN202410165045.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-02-05
Publication Date
2026-02-03
Estimated Expiration
2044-02-05

AI Technical Summary

Technical Problem

The existing bio-enzyme treatment process for tobacco leaves lacks standards and is complex, resulting in high reproducibility, huge workload, and small sample size per test, making it difficult to achieve rapid evaluation and application.

Method used

Design a highly integrated bio-enzyme processing device, comprising a left-side chamber, a relay chamber, and a right-side chamber, with several independent chambers for fermentation, inactivation, and shipping, equipped with a multi-joint robotic arm and a sterile fresh air system to achieve automated transfer and processing of tobacco leaves.

Benefits of technology

It has achieved automation, environmental consistency, and high speed in the tobacco processing process, making it suitable for single-time, small-batch, high-frequency experiments of various types, thus improving experimental efficiency and data acquisition capabilities.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application provides a device and method for rapidly processing tobacco leaves by using biological enzymes, which comprises a left box, a relay box and a right box; the left box and the right box are respectively spliced on the two sides of the relay box and are sealed at the splicing positions of the relay box; a plurality of space-independent chambers are arranged in the left box and the right box, and the plurality of chambers are divided into a plurality of fermentation chambers, at least one inactivation chamber and at least one delivery chamber; each chamber is provided with a movable sealing door for communicating or isolating the inside of the relay box on the side close to the relay box, and the inside sealing chamber formed by the left box, the relay box and the right box is a sterile chamber; a multi-joint mechanical hand is installed in the relay box and is used for realizing the transfer of the shelves in different chambers. The device and method for rapidly processing tobacco leaves by using biological enzymes have the advantages of high integration, high-frequency, small-grain and repetitive biological enzyme processing, and improved experimental efficiency.
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Description

Technical Field

[0001] This invention relates to the field of tobacco processing technology, and more specifically, to a device and method for rapid bio-enzyme processing of tobacco leaves. Background Technology

[0002] The raw materials for cigarettes are key to the quality of tobacco products, and the quality of tobacco leaves directly determines the taste and aroma of the smoke.

[0003] Tobacco leaf cell walls contain a large number of macromolecules, such as lignin, cellulose, starch, pectin, protein, and cutin. Excessive amounts of these macromolecules can cause unpleasant taste and increased irritation in cigarette products. The addition of bio-enzymes can not only break down these macromolecules in tobacco leaf cell walls but also produce smaller molecules such as sugars and aroma molecules, rapidly and significantly improving tobacco leaf quality. Therefore, using bio-enzyme treatment to improve tobacco leaf quality has become one of the important methods for enhancing tobacco product quality.

[0004] Currently, the tobacco processing of bio-enzymes suffers from a lack of standards and complex processes, which severely restricts the rapid evaluation and application of bio-enzymes.

[0005] As participants in tobacco research and standardization, the entire standardization process requires numerous cycles of bio-enzyme treatment of tobacco leaves to obtain a large number of parameters, which in turn revise relevant technical standards. This process is highly repetitive and involves a huge workload, but the number of samples used each time is small. The core demand is to automate the entire processing process, standardize the processing environment, and accelerate the processing speed. Designing a dedicated device and process that meets these requirements is a technical problem that urgently needs to be solved in this field.

[0006] In order to solve the above problems, people have been seeking an ideal technological solution. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a device and method for rapid bio-enzyme treatment of tobacco leaves that is highly integrated, capable of performing high-frequency repetitive bio-enzyme treatment processes, and improves experimental efficiency.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is: a device for rapid biological enzyme treatment of tobacco leaves, comprising a left side chamber, a relay chamber, and a right side chamber;

[0009] The left and right boxes are respectively spliced ​​to both sides of the relay box and sealed at the splice point of the relay box;

[0010] The left and right boxes are provided with several spatially independent chambers, which are divided into several fermentation chambers, at least one inactivation chamber and at least one shipping chamber.

[0011] Each chamber is equipped with a movable sealing door on the side near the relay box for connecting or isolating the interior of the relay box. The internal sealed chamber formed by the left box, the relay box and the right box is a sterile chamber.

[0012] The fermentation chamber is equipped with a temperature and humidity control mechanism and a temperature and humidity detection sensor.

[0013] The inactivation chamber is equipped with a heating component and a temperature and humidity detection sensor;

[0014] The fermentation chamber, inactivation chamber, and discharge chamber are equipped with shelves and storage spaces of the same specifications.

[0015] The relay box is equipped with a multi-joint robotic arm, which is used to transfer the shelves in different chambers between the fermentation chamber, the inactivation chamber and the shipping chamber.

[0016] At least the fermentation chamber and the discharge chamber are provided with openable and closed doors that connect to the outside.

[0017] Based on the above, the gripping end of the multi-joint manipulator includes a tray, a positioning post, and an electrically controlled movable pin. The positioning post is vertically disposed at the front end of the tray, and the electrically controlled movable pin is disposed on one side of the tray and performs a horizontal insertion action.

[0018] The shelves are arranged vertically at intervals in different chambers, and the shelf is provided with a positioning hole and a socket on the side near the movable sealing door;

[0019] The positioning hole engages with the positioning post, and the socket engages with the movable pin, thereby achieving the connection and locking between the gripping end of the multi-joint robot and the shelf.

[0020] Based on the above, each chamber is provided with a multi-layer support for the corresponding shelf. The shelf includes a central grid and surrounding positioning edges. Each layer of the support is provided with a support part and a limiting post corresponding to the positioning edge of the shelf. The positioning edge of the shelf is provided with a limiting hole matching the limiting post.

[0021] Based on the above, the movable sealing door is a horizontally moving side-sliding door, and the chamber has an opening on the side closest to the relay box. The side-sliding door is opened and closed by an electrically controlled push rod, a pneumatic cylinder, or a hydraulic cylinder.

[0022] Based on the above, the inactivation chamber is equipped with an openable and closed door that connects to the outside.

[0023] Based on the above, the fermentation chamber and the inactivation chamber are equipped with sterilization mechanisms.

[0024] Based on the above, both the fermentation chamber and the inactivation chamber are equipped with independent circulating fans and independent sterile fresh air systems. The independent sterile fresh air systems are used to regulate the temperature and humidity of the fermentation chamber and the inactivation chamber, thereby forming a temperature and humidity regulating mechanism in the fermentation chamber and a heating component in the inactivation chamber.

[0025] Based on the above, both the fermentation chamber and the inactivation chamber are equipped with independent circulating fans and independent sterile fresh air systems. The independent sterile fresh air systems are used to regulate the temperature and humidity of the fermentation chamber, thus forming a temperature and humidity regulating mechanism in the fermentation chamber. The heating component in the inactivation chamber is an independent heating component.

[0026] A method for rapidly treating tobacco leaves with bio-enzymes, comprising the aforementioned bio-enzyme rapid tobacco leaf treatment device, and the treatment process including the following steps:

[0027] Step 1) Prepare tobacco leaf samples for enzyme treatment and blank control treatment, with each type of tobacco leaf sample having the same weight;

[0028] Step 2) Add water to the tobacco leaf samples to adjust the total moisture content of the tobacco leaf samples to between 20% and 25%;

[0029] Step 3) Weigh the corresponding proportion of enzyme preparation according to the weight of the tobacco leaf sample, dissolve it repeatedly with sterile water, and then spray it evenly on the tobacco leaf sample that needs to be treated with enzyme preparation. The tobacco leaf sample for blank control treatment is sprayed with an equal amount of sterile water.

[0030] Step 4) After the treated tobacco leaf samples are sealed in a sealed bag, they are placed in the designated shelf in the fermentation chamber for constant temperature fermentation at a temperature of 32°C - 37°C for a reaction time of 4-8 hours.

[0031] Step 5) After fermentation, open the movable sealing door of the fermentation chamber and the movable sealing door of the inactivation chamber in sequence. Use a multi-joint robotic arm to transfer the corresponding rack to the inactivation chamber, close the movable sealing door, and perform high-temperature inactivation at 85°C or above for at least 30 minutes to inactivate the enzymes in the tobacco sample.

[0032] Step 6) After inactivation, open the movable sealed doors of the inactivation chamber and the shipping chamber in sequence, and transfer the inactivated tobacco leaf sample along with the rack to the shipping chamber using a multi-joint robotic arm. Set the temperature and humidity balance for a set time to make the moisture content of the tobacco leaf reach 12%-15%, then ship it out for subsequent shredding, rolling and sensory evaluation.

[0033] Based on the above, the weight of the enzyme is one-thousandth of the weight of the tobacco leaf sample, and the initial weight of the tobacco leaf sample is 20g-30g; the constant temperature and humidity of the discharge chamber is 20°C-25°C, and the relative humidity is 60%-65%.

[0034] This invention has outstanding substantive features and significant progress compared to the prior art. Specifically, this invention has the following advantages:

[0035] 1. A closed, modular box structure is designed with several independent chambers, all of which, when opened, are within a single, sterile, enclosed space. Different functions are implemented in each chamber, including fermentation, inactivation, and balanced discharge. An automated robotic arm transfers the tobacco leaves between the chambers according to a pre-defined sequence, achieving fully automated operation of the fermentation, inactivation, and discharge processes. This device is suitable for automated operation of single-batch, low-volume, high-frequency, and multi-type comparative experiments. With the assistance of this integrated equipment, a large amount of data can be obtained rapidly in a short time, providing significant advantages for analysis, research, and the development of standardized application parameters.

[0036] 2. All transfers between chambers are performed inside the enclosure. The chambers are isolated during operation but connected during transfer. During maintenance or intermittent work, the cavities are disinfected and sterilized to ensure operation in a sterile environment. This ensures uniform environmental parameters throughout the experimental process and guarantees the effectiveness of experimental comparisons. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the overall structure of the device for rapid bio-enzyme treatment of tobacco leaves in this invention.

[0038] Figure 2 This is a schematic diagram of the multi-joint robotic arm's transfer motion in this invention.

[0039] Figure 3 This is a schematic diagram of the internal structure of the fermentation chamber in this invention.

[0040] Figure 4 This is a schematic diagram of the structure of the multi-joint robotic arm and the shelf in this invention.

[0041] Figure 5 This is a schematic diagram of the gripping end of the multi-joint robotic arm in this invention.

[0042] In the diagram: 1. Left side enclosure; 2. Relay enclosure; 3. Right side enclosure; 4. Multi-joint robotic arm; 5. Shelf; 6. Support frame;

[0043] 10. Door for opening and closing; 11. Fermentation chamber; 12. Inactivation chamber; 13. Discharge chamber; 14. Temperature and humidity sensor; 15. Aseptic fresh air system; 16. Circulating fan;

[0044] 21. Movable sealed door; 22. Electric actuator; 23. Ultraviolet lamp;

[0045] 41. Pallet; 42. Positioning pin; 43. Electrically controlled movable pin;

[0046] 51. Central grid; 52. Positioning edge; 53. Limiting hole; 54. Positioning hole; 55. Socket;

[0047] 61. Support section; 62. Limiting column. Detailed Implementation

[0048] The technical solution of the present invention will be further described in detail below through specific embodiments.

[0049] like Figures 1-5 As shown, a device for rapid bio-enzyme treatment of tobacco leaves includes a left-side chamber 1, a relay chamber 2, and a right-side chamber 3.

[0050] The left box 1 and the right box 3 are respectively spliced ​​on both sides of the relay box 2 and sealed at the splice of the relay box 2. In this embodiment, the left box 1, the relay box 2 and the right box 3 are sealed together at the splice points by sealing strips and other structures.

[0051] The left box 1 and the right box 3 are provided with several spatially independent chambers. The chambers are divided into several fermentation chambers 11, at least one inactivation chamber 12 and at least one discharge chamber 13. In this embodiment, since the fermentation process is the most time-consuming, it is set to three fermentation chambers 11, one inactivation chamber 12 and one discharge chamber 13. The fermentation chambers 11 are all located in the left box 1, and the inactivation chambers 12 and the discharge chamber 13 are integrated in the right box 3.

[0052] Each chamber is provided with a movable sealing door 21 on the side near the relay box 2 for connecting or isolating the interior of the relay box 2. The movable sealing door 21 is a sliding door, driven by an independent electric push rod, pneumatic push rod or hydraulic rod. In this embodiment, an electric push rod 22 is used. A horizontal sliding track is provided on the side wall of the box corresponding to the sliding door.

[0053] The internal sealed chamber formed by the left box 1, the relay box 2, and the right box 3 is a sterile chamber, so that it is not disturbed by other environments during fermentation, transfer, and inactivation.

[0054] The fermentation chamber 11 is equipped with a temperature and humidity regulation mechanism and a temperature and humidity detection sensor 14. In this embodiment, the temperature and humidity regulation mechanism relies on a sterile fresh air system 15 independently configured in each fermentation chamber 11 to achieve temperature control at around 34°C. It is also equipped with a circulating fan 16 for airflow inside the fermentation chamber 11, which ensures a balanced temperature distribution and is beneficial to the stability of the fermentation process.

[0055] The inactivation chamber 12 is equipped with a heating component and a temperature and humidity detection sensor 14. The inactivation chamber 12 is also equipped with a sterile fresh air system 15 and a circulating fan 16. The sterile fresh air system is mainly used for the renewal and circulation of air in the inactivation chamber, and the circulating fan 16 is mainly used to maintain the temperature stability inside the inactivation chamber 12. The heating component can have two modes: one is to rely on the fresh air provided by the sterile fresh air system 15 for heating, and the heating temperature is maintained at 85°C; the other is to rely on an independent heating component for heating. The heating component can be a commonly used heating mode such as an electric heating wire or a heat exchange tube.

[0056] The fermentation chamber 11, inactivation chamber 12, and discharge chamber 13 are equipped with shelves 5 of the same specifications and shelf storage spaces. Each chamber has multiple layers of supports 6 corresponding to the shelves 5. The shelves 5 include a central grid 51 and surrounding positioning edges 52. Each layer of the support 6 is equipped with a support part 61 and a limiting post 62 corresponding to the positioning edge 52 of the shelves 5. The positioning edge 52 of the shelves 5 is equipped with a limiting hole 53 matching the limiting post 62 for placing and locking the position of the shelves 5 relative to the supports 6.

[0057] The relay box 2 is equipped with a multi-joint manipulator 4, which is used to transfer the shelf 5 in different chambers between the fermentation chamber 11, the inactivation chamber 12 and the shipping chamber 13. Specifically, the gripping end of the multi-joint manipulator 4 includes a tray 41, a positioning post 42 and an electrically controlled movable pin 43. In this embodiment, there are two positioning posts 42. The positioning posts 42 are vertically arranged at the front end of the tray 41, and the electrically controlled movable pin 43 is arranged on one side of the tray 41 and performs a horizontal insertion action.

[0058] The shelves 5 are vertically spaced on the supports 6 of different chambers. The shelf 5 is provided with a positioning hole 54 and a socket 55 on the side near the movable sealing door 21. The positioning hole 54 cooperates with the positioning post 42, and the socket 55 cooperates with the movable pin 43 to realize the connection and locking between the gripping end of the multi-joint manipulator 4 and the shelf 5.

[0059] Specifically, when the multi-joint robotic arm 4 moves to the shelf 5, it first enters the bottom of the shelf 5, and then moves from bottom to top, so that the positioning post 42 enters the positioning hole 54 on the positioning edge 52. After moving into place, it controls the movement of the electrically controlled movable pin 43 to insert into the socket 55, locking the shelf 5 at the gripping end of the multi-joint robotic arm 4, so that the transfer action can be performed.

[0060] At least the fermentation chamber 11 and the discharge chamber 13 are provided with opening and closing doors 10 that connect to the outside world, so as to facilitate the storage and retrieval of experimental samples. In this embodiment, the inactivation chamber 12 is also provided with opening and closing doors 10 that connect to the outside world, so as to perform corresponding operations during the inactivation process.

[0061] To maintain the cleanliness of the fermentation chamber 11 and the inactivation chamber 12, sterilization mechanisms are provided in the fermentation chamber 11 and the inactivation chamber 12. In this embodiment, an ultraviolet lamp 23 is used to sterilize the interior of the chamber during the maintenance period.

[0062] This paper takes the method of rapid bio-enzyme treatment of tobacco leaves as an example to illustrate the operation process of the entire equipment for rapid bio-enzyme treatment of tobacco leaves.

[0063] The following steps are required for processing:

[0064] Step 1) Prepare tobacco leaf samples for enzyme treatment and blank control treatment. The weight of each treatment tobacco leaf sample is the same. In this example, 20g is selected. In other examples, a value between 20g and 25g can also be selected. Its feature is that the amount is small, the number of groups is large, and more comparative data have been formed.

[0065] Step 2) Add water to the tobacco leaf samples to adjust the total moisture content of the samples to between 20% and 25%, so that they have the same properties and ensure that the baseline for subsequent experiments is the same.

[0066] Step 3) Weigh the enzyme preparation according to the weight of the tobacco leaf sample. In this example, one-thousandth of the weight of the tobacco leaf is selected as the enzyme preparation. It is repeatedly dissolved with sterile water and then evenly sprayed onto the tobacco leaf sample that needs to be treated with the enzyme preparation. The tobacco leaf sample of the blank control is sprayed with an equal amount of sterile water.

[0067] Step 4) After the treated tobacco leaf samples are sealed in a sealed bag, they are placed in the designated shelf of the fermentation chamber 11 for constant temperature fermentation at a temperature of 32°C - 37°C and a reaction time of 4-8 hours.

[0068] Considering the large number of chambers and shelves in fermentation chamber 11, the multi-joint robotic arm will be intelligently controlled during the automated transfer and operation process. The processing time for each shelf will be statistically analyzed, enabling the multi-joint robotic arm to sort and transfer the shelves that need to be transferred based on the statistical time, thereby ensuring the automation of the tobacco sample processing process.

[0069] Step 5) After fermentation is completed, open the movable sealing door of fermentation chamber 11 and the movable sealing door of inactivation chamber 12 in sequence. Use the multi-joint robotic arm 4 to transfer the corresponding rack 5 to inactivation chamber 12, close the movable sealing door, and perform high-temperature inactivation at 85°C or above for at least 30 minutes to inactivate the enzymes in the tobacco sample.

[0070] Generally, multiple shelves are transferred at a time to improve the utilization efficiency of each chamber of the equipment.

[0071] Step 6) After inactivation, open the movable sealed doors of the inactivation chamber 12 and the discharge chamber 13 in sequence, and transfer the inactivated tobacco leaf sample along with the rack 5 to the discharge chamber 13 using the multi-joint robotic arm 4. Set the temperature and humidity (temperature 20°C-25°C, relative humidity 60%-65%) balance time to make the moisture content of the tobacco leaf reach 12%-15% before discharge.

[0072] Step 7) Cut the balanced tobacco leaves into shreds using a tobacco sample preparation instrument; roll the cut tobacco shreds into single-material cigarettes using a small cigarette rolling machine; place the cigarettes in a constant temperature and humidity chamber (temperature 20℃, relative humidity 60%) for 24 hours to balance; the balanced cigarettes are used for storage or for sensory evaluation.

[0073] This device allows for the setting of plans and control of the time tobacco samples are stored in the fermentation chamber 11, enabling continuous batch bio-enzyme treatment experiments with multiple batches and small weights. The intermediate processes, such as transfer, time control, inactivation, equilibration, and shipping, are all automated, freeing up the hands of experimental personnel, improving experimental efficiency, facilitating the acquisition of a large number of parameters, and ensuring the stability and consistency of the experimental environment, thus guaranteeing the uniformity of experimental standards.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of the present invention or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solutions of the present invention, and all such modifications and substitutions should be covered within the scope of the technical solutions claimed in the present invention.

Claims

1. A device for rapid bio-enzyme treatment of tobacco leaves, characterized in that: Includes the left-side enclosure, the relay enclosure, and the right-side enclosure; The left and right boxes are respectively spliced ​​to both sides of the relay box and sealed at the splice point of the relay box; The left and right boxes are provided with several spatially independent chambers, which are divided into several fermentation chambers, at least one inactivation chamber and at least one shipping chamber. Each chamber is equipped with a movable sealing door on the side near the relay box for connecting or isolating the interior of the relay box. The internal sealed chamber formed by the left box, the relay box and the right box is a sterile chamber. The fermentation chamber is equipped with a temperature and humidity control mechanism and a temperature and humidity detection sensor. The inactivation chamber is equipped with a heating component and a temperature and humidity detection sensor; The fermentation chamber, inactivation chamber, and discharge chamber are equipped with shelves and storage spaces of the same specifications. The relay box is equipped with a multi-joint robotic arm, which is used to transfer the shelves in different chambers between the fermentation chamber, the inactivation chamber and the shipping chamber. At least the fermentation chamber and the discharge chamber are provided with openable and closed doors that connect to the outside.

2. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 1, characterized in that: The gripping end of the multi-joint manipulator includes a tray, a positioning post, and an electrically controlled movable pin. The positioning post is vertically arranged at the front end of the tray, and the electrically controlled movable pin is arranged on one side of the tray and performs a horizontal insertion action. The shelves are arranged vertically at intervals in different chambers, and the shelf is provided with a positioning hole and a socket on the side near the movable sealing door; The positioning hole engages with the positioning post, and the socket engages with the movable pin, thereby achieving the connection and locking between the gripping end of the multi-joint robot and the shelf.

3. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 2, characterized in that: Each chamber has a multi-layer support for the corresponding shelf. The shelf includes a central grid and surrounding positioning edges. Each layer of the support has a support part and a limiting post corresponding to the positioning edge of the shelf. The positioning edge of the shelf is provided with a limiting hole matching the limiting post.

4. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 3, characterized in that: The movable sealing door is a horizontally moving side-sliding door. The chamber has an opening on the side closest to the relay box. The side-sliding door is opened and closed by an electrically controlled push rod, a pneumatic cylinder, or a hydraulic cylinder.

5. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 4, characterized in that: The inactivation chamber is equipped with an openable and closed door that connects to the outside.

6. The apparatus for rapid bio-enzyme treatment of tobacco leaves according to any one of claims 1-5, characterized in that: The fermentation chamber and the inactivation chamber are equipped with sterilization mechanisms.

7. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 6, characterized in that: Both the fermentation chamber and the inactivation chamber are equipped with independent circulating fans and independent sterile fresh air systems. The independent sterile fresh air systems are used to regulate the temperature and humidity of the fermentation chamber and the inactivation chamber, thereby forming a temperature and humidity regulating mechanism in the fermentation chamber and a heating component in the inactivation chamber.

8. The device for rapid bio-enzyme treatment of tobacco leaves according to claim 6, characterized in that: Both the fermentation chamber and the inactivation chamber are equipped with independent circulating fans and independent sterile fresh air systems. The independent sterile fresh air systems are used to regulate the temperature and humidity of the fermentation chamber, thus forming a temperature and humidity regulation mechanism in the fermentation chamber. The heating component in the inactivation chamber is an independent heating component.

9. A method for rapid bio-enzyme treatment of tobacco leaves, characterized in that: The apparatus for rapid bio-enzyme treatment of tobacco leaves according to any one of claims 1-8 comprises the following steps: Step 1) Prepare tobacco leaf samples for enzyme treatment and blank control treatment, with each type of tobacco leaf sample having the same weight; Step 2) Add water to the tobacco leaf samples to adjust the total moisture content of the tobacco leaf samples to between 20% and 25%; Step 3) Weigh the corresponding proportion of enzyme preparation according to the weight of the tobacco leaf sample, dissolve it repeatedly with sterile water, and then spray it evenly on the tobacco leaf sample that needs to be treated with enzyme preparation. The tobacco leaf sample for blank control treatment is sprayed with an equal amount of sterile water. Step 4) After the treated tobacco leaf samples are sealed in a sealed bag, they are placed in the designated shelf in the fermentation chamber for constant temperature fermentation at a temperature of 32°C - 37°C for a reaction time of 4-8 hours. Step 5) After fermentation, open the movable sealing door of the fermentation chamber and the movable sealing door of the inactivation chamber in sequence. Use a multi-joint robotic arm to transfer the corresponding rack to the inactivation chamber, close the movable sealing door, and perform high-temperature inactivation at 85°C or above for at least 30 minutes to inactivate the enzymes in the tobacco sample. Step 6) After inactivation, open the movable sealed doors of the inactivation chamber and the shipping chamber in sequence, and transfer the inactivated tobacco leaf sample along with the rack to the shipping chamber using a multi-joint robotic arm. Set the temperature and humidity balance for a set time to make the moisture content of the tobacco leaf reach 12%-15%, then ship it out for subsequent shredding, rolling and sensory evaluation.

10. The method for rapid bio-enzyme treatment of tobacco leaves according to claim 9, characterized in that: The enzyme's weight is one-thousandth of the tobacco leaf sample's weight, and the initial weight of the tobacco leaf sample is 20g-30g; the constant temperature and humidity of the discharge chamber is 20°C-25°C, and the relative humidity is 60%-65%.

Citation Information

Patent Citations

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