A hot spring water-containing composition, a method for preparing the same, and use thereof in the preparation of skin care products having a repairing effect
Through the synergistic effect of pyrroloquinoline quinone disodium salt, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate in the hot spring water composition, the problems of skin dehydration and functional impairment were solved, achieving skin cell repair and antioxidant effects, and improving skin health.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGZHOU QIAOMEI COSMETIC CO LTD
- Filing Date
- 2024-03-12
- Publication Date
- 2026-08-04
AI Technical Summary
Existing technologies are insufficient to effectively repair skin dehydration and functional impairment caused by factors such as UV radiation, blue light radiation, smoke, age, and malnutrition, leading to skin diseases such as itchy skin, pain, rosacea, and dry skin.
Using a composition containing hot spring water, and through the synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate, a composition with excellent repair and antioxidant effects was prepared, which promotes skin cell proliferation, improves cell vitality, and strengthens the skin barrier.
It achieves skin cell repair and regeneration, enhances skin barrier function, improves sensitive skin, has excellent antioxidant and soothing effects, and is safe and non-irritating.
Smart Images
Figure SMS_1 
Figure SMS_2 
Figure SMS_3
Abstract
Description
Technical Field
[0001] This invention relates to the field of cosmetic technology, specifically to a composition containing hot spring water, its preparation method, and its application in the preparation of skin care products with repairing effects. Background Technology
[0002] The skin is the largest organ in the human body. Its outermost layer is the epidermis, which consists of five layers of keratinocytes, from the outside in: stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum basale. Factors such as UV radiation, blue light radiation, smoke, age, nutrition, and health can all cause skin dehydration, impairing skin function and leading to various skin diseases such as itchy skin, painful skin, rosacea, atopic dermatitis, and dry skin.
[0003] Therefore, how to provide a repair composition has become a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention
[0004] This invention provides a hot spring water-containing composition, its preparation method, and its application in the preparation of skin care products with repairing effects. The hot spring water-containing composition, based on the addition of hot spring water, achieves excellent repairing and antioxidant effects through the synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate. It can effectively promote the proliferation of skin cells, improve cell vitality, promote the regeneration of skin cells and the repair of cell damage, strengthen the skin barrier, and improve sensitive skin.
[0005] A hot spring water composition comprising the following components in parts by weight: 0.1-0.25 parts thickener, 0.2-0.6 parts disodium pyrroloquinoline quinone, 0.2-0.8 parts hydrolyzed zinc hyaluronic acid, 0.5-1.5 parts brown rice fermentation filtrate, 1-4 parts rose hydrosol, 2-6 parts hot spring water, 4-18 parts humectant, and 65-85 parts water.
[0006] This invention combines the above-mentioned raw materials in a specific mass ratio, and with the addition of hot spring water, it obtains a composition with excellent repair and antioxidant effects through the synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate. This composition can effectively promote the proliferation of skin cells, improve cell vitality, promote the regeneration of skin cells and the repair of cell damage, strengthen the skin barrier, and improve sensitive skin.
[0007] Among them, pyrroloquinoline quinone disodium salt has excellent repair, antioxidant and soothing properties. It can simultaneously activate the cell's antioxidant signaling pathway, namely the Nrf2-ARE pathway, promote the transcription of a series of antioxidant stress genes, inhibit the activity of reactive oxygen species that promote cell aging, promote skin cell proliferation, inhibit the expression of matrix metalloproteinases in the skin, thereby inhibiting the degradation of collagen and elastin, and improving and repairing mitochondrial function.
[0008] The brown rice extract filtrate is rich in peptides, amino acids, and minerals, and has excellent antioxidant, whitening, and repairing effects. It can improve cell vitality and human immune function. The brown rice extract filtrate can penetrate deep into the skin, deeply nourish the skin, promote cell metabolism, promote cell proliferation, inject vitality into skin cells, effectively promote skin cell regeneration and repair of cell damage, promote cell migration, and at the same time improve dull skin and brighten skin tone.
[0009] The hydrolyzed zinc hyaluronic acid has moisturizing, repairing and nourishing effects on the skin, as well as excellent antioxidant and soothing effects. It can effectively repair the skin, strengthen the skin barrier and improve sensitive skin.
[0010] The hot spring water mentioned above comes from natural mineral hot springs in volcanic areas. Various minerals can soothe the skin, reduce allergies, and enhance the skin's natural protective function.
[0011] As a preferred embodiment of the present invention, it comprises the following components in parts by weight: 0.15-0.25 parts thickener, 0.3-0.5 parts disodium pyrroloquinoline quinone, 0.4-0.7 parts hydrolyzed zinc hyaluronic acid, 0.8-1.3 parts brown rice fermentation filtrate, 1-2 parts rose hydrosol, 2-5 parts hot spring water, 6-16 parts humectant, and 70-85 parts water.
[0012] As a preferred embodiment of the present invention, it comprises the following components in parts by weight: 0.2 parts thickener, 0.4 parts disodium pyrroloquinoline quinone, 0.6 parts hydrolyzed zinc hyaluronic acid, 1 part brown rice fermentation filtrate, 1.5 parts rose hydrosol, 4 parts hot spring water, 13 parts humectant, and 79.3 parts water.
[0013] In a preferred embodiment of the present invention, the thickener is at least one of xanthan gum, sclerotinia gum, carrageenan, and carbomer.
[0014] As a preferred embodiment of the present invention, the moisturizer includes at least one of 1,2-hexanediol, propylene glycol, butylene glycol, glycerin, pentanediol, xylitol, and sorbitol.
[0015] The present invention also provides a method for preparing a composition containing hot spring water, comprising the following steps: Thickener, disodium pyrroloquinoline quinone, and humectant were added to water and stirred until homogeneous. Then, hydrolyzed zinc hyaluronic acid, brown rice fermentation filtrate, rose hydrosol, and hot spring water were added and stirred until homogeneous to obtain a hot spring water-containing composition.
[0016] The present invention also provides an application of a hot spring water-containing composition in the preparation of skin care products with repairing effects.
[0017] In a preferred embodiment of the present invention, the skin care product with repairing effect contains 0.1% to 10% by mass of the hot spring water composition.
[0018] In a preferred embodiment of the present invention, the skin care product with repairing effect contains 1-5% by mass of the hot spring water composition.
[0019] As a preferred embodiment of the present invention, the dosage form of the skin care product is at least one of serum, mask, cream, and lotion.
[0020] The beneficial effects of this invention are as follows: Based on the addition of hot spring water, this invention obtains a composition with excellent repair and antioxidant effects through the synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate. It can effectively promote the proliferation of skin cells, improve cell vitality, promote the regeneration of skin cells and the repair of cell damage, enhance the skin barrier, and improve sensitive skin. Detailed Implementation
[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0022] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.
[0023] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.
[0024] In this invention, there are no particular limitations on the specific dispersion and stirring methods.
[0025] Unless otherwise specified, all reagents or instruments used in this invention are commercially available products. The raw materials used in the examples and comparative examples are described below: Disodium pyrroloquinoline quinone: Water sheep organism.
[0026] Hydrolyzed zinc hyaluronic acid: Bloomage Biotechnology.
[0027] Brown rice fermentation filtrate: Bloomage Biotechnology.
[0028] Hot spring water: Huilang Biology, GOLDEN MOUNTAIN SPRING WATERS (inci hot spring water).
[0029] Unless otherwise specified, all components and raw materials used in the embodiments and comparative examples of this invention are commercially available, and the same type of components and raw materials are used in each parallel experiment.
[0030] Examples and comparative examples: The components and weight parts of the compositions containing hot spring water in the examples and comparative examples are shown in Tables 1 and 2 (all weight parts are shown in the tables).
[0031] The preparation methods of the compositions containing hot spring water in the examples and comparative examples are as follows: Xanthan gum, disodium pyrroloquinoline quinone, propylene glycol, and glycerin were added to water and stirred until homogeneous. Then, hydrolyzed zinc hyaluronic acid, brown rice fermentation filtrate, rose hydrosol, and hot spring water were added and stirred until homogeneous to obtain a hot spring water-containing composition (in which, the comparative proportions were added according to the ratio of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate).
[0032] Table 1
[0033] Table 2
[0034] Test case
[0035] Test Example 1
[0036] Stability tests were conducted on the examples and comparative examples. The heat resistance test involved standing at 45±2℃ for one month, observing the appearance, color, and odor. The cold resistance test involved standing at -18±2℃ for one month, observing the appearance, color, and odor. The thermal cycling test involved storing the samples at 45℃, room temperature, and -18℃ for 24 hours each, cycling three times, and observing the appearance, color, and odor. The test results are shown in Table 3.
[0037] Table 3
[0038] As can be seen from Table 3, the hot spring water-containing composition of the present invention exhibits excellent stability.
[0039] Test Example 2 HaCaT (human keratinocytes) were cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin at 37°C, 5% CO2, and 98% humidity. Logarithmic growth phase cells were selected and seeded in 96-well plates. After 24 hours of complete cell adhesion, the medium was replaced with RPMI-1640 containing the samples from the examples and comparative examples (final concentration 0.1 mg / mL), and incubated for 12 hours. The culture was then incubated with 30 mJ / cm² water. 2 Cells were irradiated with UVB at a distance of 15 cm. After irradiation, the cell culture supernatant was collected, and the concentration of IL-1α in the supernatant was detected using a human ELISA kit. Each experiment was repeated three times, and the average value was taken as the final result. The results are shown in Table 4.
[0040] Test Example 3 Prepare a 1 mg / mL sample solution using distilled water from the compositions of Example 1 and the comparative example. Prepare a 0.12 mg / mL DPPH ethanol solution using 95% ethanol. Add 2 mL of the DPPH standard solution to a test tube, mix thoroughly, and incubate at room temperature for 30 min. Measure the absorbance (A) at 517 nm. Repeat each experiment three times, and take the average value as the final result. Determine the DPPH scavenging rate using the following formula; the results are shown in Table 4.
[0041] DPPH removal rate = [1 - (A1 - A2) / A0] × 100%; A1: Absorbance of 2 mL sample solution + 2 mL DPPH solution; A2: Absorbance of 2 mL sample solution + 2 mL 95% ethanol; A0: Absorbance of 2 mL 95% ethanol + 2 mL DPPH solution.
[0042] Table 4
[0043] As can be seen from Tables 1 to 4, the compositions of the present invention have excellent antioxidant and repair effects.
[0044] Comparing Examples 1 to 5, it can be seen that Example 1 is the best implementation of the present invention, with the best antioxidant and repair effects. The weights of each raw material are as follows: 0.15 to 0.25 parts thickener, 0.3 to 0.5 parts disodium pyrroloquinoline quinone, 0.4 to 0.7 parts hydrolyzed zinc hyaluronic acid, 0.8 to 1.3 parts brown rice fermentation filtrate, 1 to 2 parts rose hydrosol, 2 to 5 parts hot spring water, 6 to 16 parts moisturizer, and 70 to 85 parts water. When these ingredients are present, the antioxidant and repair effects are even better.
[0045] Comparing Examples 1-5 with Comparative Examples 10-11, it can be seen that different amounts of raw materials have a certain impact on the effect. By controlling the amount of each raw material within the range of the present invention, the antioxidant and repair effects are further improved.
[0046] Comparing Example 1 with Comparative Examples 1-9, it can be seen that in this invention, disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate are indispensable substances. The synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate has a synergistic effect in anti-oxidation and repair. If any one of them is missing, the anti-oxidation and repair effects will be significantly reduced.
[0047] Comparing Example 1 and Comparative Example 12, it can be seen that the present invention, by adding hot spring water, obtains a composition with excellent repair and antioxidant effects through the synergistic effect of disodium pyrroloquinoline quinone, hydrolyzed zinc hyaluronic acid, and brown rice fermentation filtrate.
[0048] Test Example 4 Ten healthy subjects aged 20 to 30 years with no skin diseases or allergies were selected. The human skin patch test was conducted on the composition of Example 1 in accordance with the human skin patch test method specified in the "Cosmetic Safety Technical Specifications" (2015 edition).
[0049] Select qualified patch material, and place 0.02±0.002g of sample into the patch applicator. The control area is blank (without any substance). Apply both the sample and the blank control to the flexor side of the subject's forearm, and gently press with the palm to ensure even application to the skin. The test substance was removed 24 hours later. The skin condition of the sample and control areas was examined at 0.5 hours, 24 hours and 48 hours after the test substance plaque was removed.
[0050] Grading criteria for adverse skin reactions in the test area: 0 points for negative reaction; 1 point for questionable: only slight erythema; 2 points for weak positive reaction: erythema, infiltration, edema, and possible papules; 3 points for strong positive reaction: erythema, infiltration, edema, and possible papules, with the reaction extending beyond the test area; 4 points for extremely strong positive reaction: obvious erythema, severe infiltration, edema, and confluent herpes, with the reaction extending beyond the test area. The test results are shown in Table 5.
[0051] Table 5
[0052] As can be seen from Table 5, the composition described in this invention has excellent safety, is mild and non-irritating, and will not cause adverse reactions.
[0053] Based on the above-described preferred embodiments of the present invention, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the technical spirit of the present invention. The technical scope of the present invention is not limited to the contents of the specification, but must be determined according to the scope of the claims.
Claims
1. A composition containing hot spring water, characterized in that, It contains the following components in parts by weight: 0.1-0.25 parts thickener, 0.2-0.6 parts disodium pyrroloquinoline quinone, 0.2-0.8 parts hydrolyzed zinc hyaluronic acid, 0.6-1.5 parts brown rice fermentation filtrate, 1-4 parts rose hydrosol, 2-6 parts hot spring water, 4-18 parts humectant, and 65-85 parts water; The brown rice fermentation filtrate was purchased from Bloomage Biotechnology; the hot spring water was purchased from Huilang Biotechnology.
2. The hot spring water composition according to claim 1, characterized in that, It contains the following components by weight: 0.15-0.25 parts thickener, 0.3-0.5 parts disodium pyrroloquinoline quinone, 0.4-0.7 parts hydrolyzed zinc hyaluronic acid, 0.8-1.3 parts brown rice fermentation filtrate, 1-2 parts rose hydrosol, 2-5 parts hot spring water, 6-16 parts humectant, and 70-85 parts water.
3. The hot spring water composition according to claim 1, characterized in that, It contains the following components by weight: 0.2 parts thickener, 0.4 parts disodium pyrroloquinoline quinone, 0.6 parts hydrolyzed zinc hyaluronic acid, 1 part brown rice fermentation filtrate, 1.5 parts rose hydrosol, 4 parts hot spring water, 13 parts humectant, and 79.3 parts water.
4. The hot spring water composition according to claim 1, characterized in that, The thickener is at least one of xanthan gum, sclerotinia gum, carrageenan, and carbomer.
5. The hot spring water composition according to claim 1, characterized in that, The humectant includes at least one of 1,2-hexanediol, propylene glycol, butylene glycol, glycerin, pentanediol, xylitol, and sorbitol.
6. The method for preparing the hot spring water composition according to any one of claims 1 to 5, characterized in that, Includes the following steps: Thickener, disodium pyrroloquinoline quinone, and humectant were added to water and stirred until homogeneous. Then, hydrolyzed zinc hyaluronic acid, brown rice fermentation filtrate, rose hydrosol, and hot spring water were added and stirred until homogeneous to obtain a hot spring water-containing composition.
7. The use of the hot spring water composition according to any one of claims 1 to 6 in the preparation of skin care products with repairing effects.
8. The application according to claim 7, characterized in that, The skin care product with repairing effect contains 0.1% to 10% by mass of the hot spring water composition.
9. The application according to claim 8, characterized in that, The skin care product with repairing effect contains 1-5% by mass of the hot spring water composition.
10. The application according to claim 7, characterized in that, The skincare product is in the form of at least one of serum, mask, cream, or lotion.