A traditional Chinese medicine composition for treating Weijian polypeptide expression hyperplasia of stomach and its preparation method and application

Abstract

The present application belongs to the technical field of traditional Chinese medicine, and relates to a traditional Chinese medicine composition for treating gastric mucosa metaplasia, a preparation method and application thereof. The traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 9-18 parts of ginseng, 9-18 parts of white atractylodes, 9-18 parts of poria cocos, and 6-9 parts of licorice (stir-fried). The traditional Chinese medicine composition prepared by the present application can reduce damage to gastric mucosa glands, reduce oxidative stress damage, enhance antioxidant capacity, protect gastric mucosa glands, and prevent gastric mucosa metaplasia from further malignant transformation. The traditional Chinese medicine composition is safe and has no obvious toxic and side effects.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for treating gastric mucosal metaplasia, its preparation method, and its application. Background Technology

[0002] Gastric mucosal metaplasia refers to changes in the normal cell types of the gastric mucosa, usually caused by long-term chronic irritation or injury. It is currently widely believed that gastric mucosal metaplasia may be related to factors such as inflammation, infection, and atrophy. Long-term metaplasia may lead to gastric cancer and is recognized as one of the important precancerous lesions of the stomach.

[0003] Studies have shown that spasmolytic polypeptide expressing metaplasia (SPEM), as an alternative to intestinal metaplasia, plays a crucial role in the occurrence and progression of gastric cancer. Gastric mucosal tissue undergoes oxidative damage under the continuous stimulation of chronic inflammation, leading to SPEM, which can then transdifferentiate into incomplete intestinal metaplasia. This may be one of the mechanisms underlying gastric adenocarcinoma. Because the occurrence of SPEM is closely related to gastric mucosal carcinogenesis, it has attracted widespread attention in recent years. However, there is currently a lack of research and methods to block SPEM, further preventing this pathological process of gastric mucosal metaplasia from transforming into malignancy.

[0004] Therefore, developing and finding effective treatments will help in the early prevention and treatment of gastric cancer, and may help reduce the incidence and mortality of intestinal-type gastric cancer. Summary of the Invention

[0005] In view of the problems existing in the prior art, the present invention provides a traditional Chinese medicine composition for treating gastric mucosal metaplasia, its preparation method and application. In animal experiments, the present invention constructed a mouse model of gastric mucosal metaplasia—an antispasmodic peptide-expressing metaplasia—using tamoxifen administration, verifying a traditional Chinese medicine composition commonly used to treat spleen and stomach qi deficiency syndrome. This composition can improve the pathological state of gastric mucosal metaplasia by promoting the expression of antioxidant genes, reducing oxidative stress, and alleviating oxidative damage.

[0006] Antispasmodic peptide expression, as a second type of metaplasia associated with intestinal-type gastric cancer and considered one of the possible precancerous lesions of the gastric mucosa, is currently receiving increasing attention. To further prevent the pathological process of this gastric mucosal metaplasia transforming into malignancy, this invention provides a traditional Chinese medicine composition and its preparation method.

[0007] A traditional Chinese medicine composition for treating gastric mucosal metaplasia includes the following traditional Chinese medicine components in parts by weight: ginseng 9-18 parts, atractylodes macrocephala 9-18 parts, poria cocos 9-18 parts, and licorice (stir-fried) 6-9 parts.

[0008] The above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia can be prepared into decoctions, pills, ointments, tablets, capsules, granules, oral solutions, and powders, etc.

[0009] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia includes the following steps when the traditional Chinese medicine composition is an extract: (1) weigh the raw materials according to the ratio; (2) soak the weighed medicinal materials in about 3 times the volume of 70% alcohol for 12 hours, filter with filter paper and funnel, retain the filtrate and discard the filter residue; (3) place the obtained filtrate on a rotary evaporator for rotary evaporation, take it out when the filtrate is viscous and clings to the wall, and place it in a hot air sterilizer to evaporate the remaining alcohol.

[0010] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia, when the traditional Chinese medicine composition is in powder form, includes the following steps: taking the above-mentioned drug combination according to the specified weight proportions, pulverizing it, and simultaneously passing it through 10-mesh and 50-mesh sieves; continuing to pulverize the coarse particles that cannot pass through the 10-mesh sieve, and taking the powder located between the 10-mesh and 50-mesh sieves; mixing all the raw material powders evenly, packing them into a packaging bag, and sealing it. When needed, the powder can be directly dissolved in 150-200ml of warm water and taken orally.

[0011] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia, when the traditional Chinese medicine composition is in the form of pills, includes the following steps: take the above-mentioned combination of drugs according to the weight proportions, crush them, and pass them through 10-mesh and 50-mesh sieves. The coarse particles that cannot pass through the 10-mesh sieve should be further crushed. Take the powder between the 10-mesh and 50-mesh sieves and dry it in the sun. Take an appropriate amount of honey, simmer it slowly over low heat, stir, skim off the impurities and foam on the surface, and wait until the temperature rises to 116℃~119℃. Mix it with the drug powder (add it at a ratio of 1:1.2~1.5), roll it into pills, each weighing about 3g. After the prepared honey pills have dried at room temperature, seal them in bags for storage. This pill is convenient to take and has a long-lasting effect.

[0012] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia includes the following steps when the traditional Chinese medicine composition is a decoction: weighing the raw materials according to the ratio; soaking the raw materials in 8-12 times their weight of water for 30 minutes, then heating to boiling and maintaining a gentle boil, separating to obtain the first decoction and the residue; taking the residue obtained after the first decoction, adding 8-10 times the weight of water equivalent to the total weight of the raw materials, heating to boiling, maintaining a gentle boil, separating to obtain the second decoction and the residue; combining the first decoction and the second decoction to obtain the decoction.

[0013] The application of the pharmaceutical composition for treating gastric mucosal metaplasia described in this invention in the preparation of a drug for treating gastric mucosal metaplasia.

[0014] This invention provides a traditional Chinese medicine composition for treating gastric mucosal metaplasia. Ginseng is the principal ingredient, possessing the effects of sweet and warm properties, invigorating qi, strengthening the spleen, and nourishing the stomach. Atractylodes macrocephala, bitter and warm, is used as the assistant ingredient to invigorate the spleen, dry dampness, and enhance the qi-invigorating and digestive effects. Poria cocos, sweet and bland, is used as the adjuvant ingredient to invigorate the spleen and eliminate dampness; the combination of Poria cocos and Atractylodes macrocephala further enhances the spleen-invigorating and dampness-eliminating effects. Licorice root is used as the guiding ingredient to invigorate qi, harmonize the middle jiao, and regulate the effects of all the other ingredients. The four herbs work together to invigorate qi and strengthen the spleen. All the herbs in this formula are mild in nature, nourishing without being drastic, and warming without being drying; they are all neutral in nature.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows.

[0016] This invention provides a traditional Chinese medicine composition for treating gastric mucosal metaplasia, which can reduce damage to gastric mucosal glands, enhance antioxidant capacity by mitigating oxidative stress, protect gastric mucosal glands, and prevent further malignant transformation of gastric mucosal metaplasia. It is safe and has no obvious toxic side effects. Furthermore, this traditional Chinese medicine composition is effective in both prevention and treatment. The composition uses natural Chinese herbal medicines and can be provided in various dosage forms for convenient use. Attached Figure Description

[0017] Figure 1 Pretreatment with a traditional Chinese medicine composition improved tamoxifen-induced gastric mucosal gland damage.

[0018] Figure 2 The traditional Chinese medicine composition improved tamoxifen-induced gastric mucosal gland damage after treatment.

[0019] Figure 3 Pretreatment with a traditional Chinese medicine composition improved tamoxifen-induced H+ / K+ ATPase in gastric mucosal gland parietal cells.

[0020] Figure 4 Pretreatment with a traditional Chinese medicine composition improved the level of reactive oxygen species (ROS) in the gastric mucosal glands induced by tamoxifen.

[0021] Figure 5 Western blot analysis was performed on the expression levels of 8-oxo-dg, HO-1, and Nrf2 proteins in the gastric tissues of mice in the Model group and the traditional Chinese medicine combination group after tamoxifen induction at 0h, 6h, 24h, 48h, and 72h.

[0022] Figure 6 H&E staining was used to observe the effects on the histopathology of mouse heart, liver, and kidney tissues. Detailed Implementation

[0023] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not limited to the scope of the present invention. Modifications of the present invention in various equivalent forms by those skilled in the art fall within the scope defined by the appended claims.

[0024] A traditional Chinese medicine composition for treating gastric mucosal metaplasia includes the following traditional Chinese medicine components in parts by weight: ginseng 9-18 parts, atractylodes macrocephala 9-18 parts, poria cocos 9-18 parts, and licorice (stir-fried) 6-9 parts.

[0025] The above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia can be prepared into decoctions, pills, ointments, tablets, capsules, granules, oral solutions, and powders, etc.

[0026] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia includes the following steps when the traditional Chinese medicine composition is an extract: (1) weigh the raw materials according to the ratio; (2) soak the weighed medicinal materials in about 3 times the volume of 70% alcohol for 12 hours, filter with filter paper and funnel, retain the filtrate and discard the filter residue; (3) place the obtained filtrate on a rotary evaporator for rotary evaporation, take it out when the filtrate is viscous and clings to the wall, and place it in a hot air sterilizer to evaporate the remaining alcohol.

[0027] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia, when the traditional Chinese medicine composition is in powder form, includes the following steps: taking the above-mentioned drug combination according to the specified weight proportions, pulverizing it, and simultaneously passing it through 10-mesh and 50-mesh sieves; continuing to pulverize the coarse particles that cannot pass through the 10-mesh sieve, and taking the powder located between the 10-mesh and 50-mesh sieves; mixing all the raw material powders evenly, packing them into a packaging bag, and sealing it. When needed, the powder can be directly dissolved in 150-200ml of warm water and taken orally.

[0028] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosal metaplasia, when the traditional Chinese medicine composition is in the form of pills, includes the following steps: take the above-mentioned combination of drugs according to the weight proportions, crush them, and pass them through 10-mesh and 50-mesh sieves. The coarse particles that cannot pass through the 10-mesh sieve should be further crushed. Take the powder between the 10-mesh and 50-mesh sieves and dry it in the sun. Take an appropriate amount of honey, simmer it slowly over low heat, stir, skim off the impurities and foam on the surface, and wait until the temperature rises to 116℃~119℃. Mix it with the drug powder (add it at a ratio of 1:1.2~1.5), roll it into pills, each weighing about 3g. After the prepared honey pills have dried at room temperature, seal them in bags for storage. This pill is convenient to take and has a long-lasting effect.

[0029] The preparation method of the above-mentioned traditional Chinese medicine composition for treating gastric mucosa metaplasia lesions, when the traditional Chinese medicine composition is a decoction, includes the following steps: weighing the raw materials according to the ratio; adding the raw materials to 8-12 times the weight of water and soaking for 30 minutes, then heating to boiling and maintaining a gentle boil, and separating to obtain the first decoction liquid and the medicinal residues; taking the medicinal residues obtained after the first decoction, adding 8-10 times the weight of water equivalent to the total mass of the raw materials, heating to boiling, maintaining a gentle boil, and separating to obtain the second decoction liquid and the medicinal residues; combining the first decoction liquid and the second decoction liquid to obtain an oral decoction.

[0030] Example 1.

[0031] A traditional Chinese medicine composition for treating gastric mucosa metaplasia lesions, with the raw materials being: 9 parts of ginseng, 9 parts of atractylodes macrocephala, 9 parts of poria cocos, and 9 parts of licorice (stir-fried).

[0032] The preparation method of the traditional Chinese medicine composition extract: (1) weighing the raw materials according to the ratio; (2) soaking the weighed medicinal materials in 70% ethanol with a volume about 3 times for 12 hours, filtering with filter paper and funnel, retaining the filtrate, and discarding the filter residues; (3) placing the obtained filtrate on a rotary evaporator for rotary evaporation, taking it out when the filtrate is in a viscous state adhering to the wall, and placing it in a hot air disinfection box to evaporate the remaining alcohol.

[0033] Effect verification of Example 2.

[0034] 1. Experimental animals.

[0035] Male C57BL / 6 mice at 6-7 weeks of age, experimental animal license number: SCXK(Su)2023-0009. They were housed in a clean-grade animal room at a temperature of 20±24°C, a relative humidity of 55±5%, with 12 hours of alternating light and darkness, and free access to food and water. The animal experiment has passed the animal experiment ethics review of China Medical University.

[0036] 2. Experimental drugs.

[0037] The traditional Chinese medicine composition of Example 1 and tamoxifen (Toronto Research Chemicals Inc, Cat#T00600), prepared with a mixed solvent of anhydrous ethanol and sunflower oil in a volume ratio of 1:9, and the final concentration was configured to be 25 mg / ml.

[0038] 3. Construction of the mouse SPEM model and administration.

[0039] (1) Prepare sterile 1.5ml centrifuge tubes. Add 900μl of sterile sunflower seed oil to centrifuge tube A. Weigh 25mg of tamoxifen into another 1.5ml centrifuge tube B. Add 100μl of anhydrous ethanol to centrifuge tube B and sonicate until the solid is completely dissolved. Then transfer the mixture in centrifuge tube B to centrifuge tube A and quickly shake to mix. Finally, prepare a 25mg / ml tamoxifen mixture. Based on the equivalent clinical dose for a 60kg adult: the converted dosage of the herbal medicine composition for mice is 5.46g / kg·d.

[0040] (2) After one week of acclimatization, C57BL / 6 mice were randomly divided into 6 groups using a random number table, including pretreatment and no pretreatment programs. Each program included a Control group, a Model group, and a traditional Chinese medicine composition group, with 10 mice in each group (n=10), and 5 mice per cage. In the pretreatment program, the Control and Model groups were treated with ddH2O (10µl / g×mouse body weight) by gavage for 8 days, and the traditional Chinese medicine composition group was treated with the traditional Chinese medicine composition (5.46g / kg·d) for 8 days. On the 6th day of pretreatment, the Model and Traditional Chinese Medicine Composition groups were injected intraperitoneally with tamoxifen (10µl / g×mouse body weight) at a final concentration of 25mg / ml for 3 days. On the 6th day of pretreatment, the Control group was injected intraperitoneally with a blank solution (ddH2O) for 3 days. In the no-pretreatment regimen, the Model group and the traditional Chinese medicine combination group were intraperitoneally injected with tamoxifen (10µl / g×mouse body weight) at a final concentration of 25mg / ml for 3 days, while the Control group was intraperitoneally injected with blank solution (ddH2O) for 3 days to model the disease. Subsequently, the Control group and the Model group were administered ddH2O (10µl / g×mouse body weight) by gavage for 3 days, while the traditional Chinese medicine combination group was treated with the traditional Chinese medicine composition (5.46g / kg·d) for 3 days.

[0041] 4. Experimental methods.

[0042] After modeling was completed in each group and drug administration was completed, the heart, liver, kidneys, and stomach tissues were dissected and separated. Hematoxylin and eosin (H&E) staining was used to assess the damage and pathological changes in the mucosal glands of the heart, liver, kidneys, and stomach. Mouse serum was collected by centrifugation, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), lactate dehydrogenase (LDH), and creatine kinase isoenzyme (CK-MB) were detected. Immunofluorescence staining was used to detect H2O in the parietal cells of the gastric mucosal glands of mice. + / K + The effect of ATPase; the level of reactive oxygen species (ROS) in mouse gastric tissue was detected by frozen section, and the oxidative stress damage in mouse gastric tissue was detected by Western blotting.

[0043] Example 3.

[0044] 1. Damage to gastric mucosal glands and the pathological condition of major organ tissues.

[0045] Mice were sacrificed after drug administration, and their heart, liver, kidneys, and stomach tissues were separated, fixed in 10% formalin, embedded in paraffin, sectioned, and stained with H&E to observe the pathological changes in the heart, liver, and kidney tissues, as well as the damage to the gastric mucosal glands.

[0046] 2. Effects on gastric parietal cells H + / K + The effect of ATPase.

[0047] Mice were sacrificed after drug administration, and their stomach tissues were isolated, fixed in 10% formalin, embedded in paraffin, sectioned, dewaxed to water, and subjected to antigen retrieval in an autoclave after adding citrate buffer (pH=6.0). After blocking with blocking buffer at room temperature for 1 hour, primary antibody (H) was added. + / K + ATPase (1:100) was incubated overnight at 4°C in a humidified chamber, followed by washing three times with PBST. Secondary antibody corresponding to the primary antibody species was added and incubated at room temperature for 1 hour, followed by washing three times with PBST. Diluted DAPI was added to the slides to cover the tissue, and the reaction was carried out at room temperature for 5 minutes, protected from light. Finally, the slides were mounted with DAPI-containing mounting medium and air-dried before being imaged under a fluorescence microscope.

[0048] 3. Effects on reactive oxygen species (ROS) in the gastric mucosa.

[0049] Mice were sacrificed after drug administration, and their gastric tissue was isolated, rapidly frozen, embedded using OCT, and frozen sections were prepared. ROS levels in the mouse gastric tissue were detected using a ROS staining kit. The BBoxiProbe® DHE reactive oxygen species fluorescent probe was diluted 1000-fold with pure water to prepare a staining probe working solution. A 10X washing buffer was diluted 10-fold with pure water to prepare a 1X washing buffer working solution. Unfixed frozen sections with a thickness of 15 μm were prepared for testing. At room temperature, 200 μl of the washing buffer working solution was carefully added, covering the entire surface of the section, and allowed to stand for 5 minutes. The washing buffer was removed. 100-200 μl of the staining probe working solution was added, and the section was incubated at 37°C in the dark for 30 minutes. The staining solution was carefully removed, and the section was washed three times with PBS and mounted with glycerol. Fluorescence intensity was detected and photographed using a fluorescence microscope at an excitation wavelength of 535 nm and an emission wavelength of 610 nm.

[0050] 4. Effects on oxidative stress in mouse gastric tissue.

[0051] After drug administration, gastric tissue was separated, homogenized, and total protein was extracted. BCA protein quantification was performed, and Western blotting was conducted to detect the expression levels of 8-oxo-dg, HO-1, and Nrf2 proteins in the gastric tissue of mice in the Model group and the traditional Chinese medicine combination group after tamoxifen induction at 0h, 6h, 24h, 48h, and 72h, respectively, with β-actin used as an internal control.

[0052] 5. Biochemical function detection of mouse serum ALT, AST, Cr, LDH, and CK-MB.

[0053] Blood was collected from the orbital cavity of mice, and 1.5 ml of blood was placed in a sterile EP tube. The tube was incubated at room temperature for 1 hour, then centrifuged at 3000g for 15 minutes at 4°C. The supernatant was transferred to a labeled sample tube for storage, which is the obtained serum. The serum ALT, AST, Cr, LDH, and CK-MB levels were measured using an automated biochemical analyzer according to the kit instructions.

[0054] Example 4 Experimental results.

[0055] 1. The traditional Chinese medicine composition improved tamoxifen-induced gastric mucosal gland damage. H&E staining histopathological results are shown in […]. Figure 1 The results showed that, compared with the control group, the model group mice exhibited significant apical-basal damage to the gastric mucosal glands, with a reduction or even disappearance of parietal cells, and gland dilation. In contrast, after gavage administration of the traditional Chinese medicine composition, the apical-basal damage to the gastric mucosa in mice was not significant, and the number and morphology of gastric parietal cells remained largely normal, indicating that the traditional Chinese medicine composition has the effect of improving tamoxifen-induced gastric mucosal gland damage.

[0056] exist Figure 2 In this study, mice were administered tamoxifen or a blank solution via intraperitoneal injection for 3 days, followed by gavage administration of ddH2O or a traditional Chinese medicine composition for 3 days, respectively. Results showed that compared to the Control group, the Model group mice exhibited significant apical-basal damage to the gastric mucosal glands, with reduced or even absent parietal cells, and gland dilation. Mice administered the traditional Chinese medicine composition showed milder apical-basal damage to the gastric mucosa, with normal numbers and morphology of gastric parietal cells.

[0057] 2. The traditional Chinese medicine composition improves the H2O level of tamoxifen-induced gastric mucosal parietal cells. + / K + ATPase, results are shown in Figure 3 As shown. H + / K + ATPase plays an important role in maintaining the normal structure of gastric parietal cells and the normal acidic environment in the stomach. + / K +Low ATPase expression leads to reduced gastric acid secretion and gastric mucosal atrophy. Compared with the Control group, the Model group and the traditional Chinese medicine composition group showed lower H levels in the parietal cells of the gastric mucosal glands. + / K + The number of ATPase-positive cells decreased to varying degrees in all groups. Compared with the Model group, the number of H cells in the gastric mucosal gland parietal cells of mice in the traditional Chinese medicine composition group was significantly reduced. + / K + The number of ATPase-positive cells has improved.

[0058] 3. The traditional Chinese medicine composition improved tamoxifen-induced oxidative damage to gastric mucosal glands after treatment, as shown in the following results. Figure 4 As shown in the figure, reactive oxygen species (ROS) levels are a key indicator of oxidative damage caused by normal cellular physiological functions and environmental factors. Therefore, reliable measurement of ROS concentration or relative levels is of great significance. By detecting ROS levels in mouse gastric tissue, the results showed that compared with the Control group, the ROS levels in the gastric mucosal glands of the Model group were significantly increased. Compared with the Model group, the ROS levels in the gastric mucosal glands of mice were significantly reduced after gavage administration of the traditional Chinese medicine composition.

[0059] 4. The traditional Chinese medicine composition reduced tamoxifen-induced oxidative damage to gastric mucosal glands and enhanced antioxidant activity after treatment. 8-hydroxydeoxyguanosine (8-oxo-dg) is the most commonly used biomarker of DNA oxidative damage. Heme oxygenase-1 (HO-1) is an important antioxidant enzyme, while Nrf2 (Nuclear factor erythroid 2-related factor 2, NFE2L2) is a very important transcription factor in oxidative stress response and can directly regulate HO-1 promoter activity. Therefore, Western blot was used to detect the protein expression levels of 8-oxo-dg, Nrf2, and HO-1. The results are as follows. Figure 5 As shown, compared with the Model group, the expression of 8-oxo-dg protein in the gastric tissue of mice was downregulated and the expression of Nrf2 and HO-1 proteins was upregulated after tamoxifen induction at 0h, 6h, 24h, 48h and 72h, respectively.

[0060] 5. Effects of ALT, AST, Cr, LDH, and CK-MB levels in mouse serum.

[0061] To further evaluate the safety of the traditional Chinese medicine composition for treatment, serum ALT, AST, Cr, LDH, and CK-MB levels in mice were measured to observe the damage to myocardial enzymes, liver function, and kidney function. The results showed that the biochemical test results of myocardial enzymes, liver function, and kidney function in each group of mice were all within the normal reference range (Table 1).

[0062] Table 1 shows the serum ALT, AST, Cr, LDH, and CK-MB levels in mice.

[0063]

[0064] 6. Effects on the histopathology of mouse heart, liver, and kidney tissues.

[0065] The histopathological condition of the heart, liver, and kidneys of mice was observed by H&E staining. The results showed that in the Control, Model, and Traditional Chinese Medicine combination groups, the heart, liver, and kidney cells were tightly arranged, clearly demarcated, with abundant cytoplasm, normal cell morphology, and uniform staining, and no obvious abnormalities were observed.

Claims

1. The use of a pharmaceutical composition for treating gastric mucosal metaplasia in the preparation of a drug for treating gastric antispasmodic peptide-expressed metaplasia; The traditional Chinese medicine composition for treating gastric mucosal metaplasia is made from the following traditional Chinese medicine raw materials in parts by weight: ginseng 9-18 parts, atractylodes macrocephala 9-18 parts, poria cocos 9-18 parts, and stir-fried licorice root 6-9 parts.

2. The method for preparing the traditional Chinese medicine composition for treating gastric mucosal metaplasia according to claim 1, characterized in that, When the traditional Chinese medicine composition is an extract, the following steps are included: (1) weigh the raw materials according to the ratio; (2) soak the weighed medicinal materials in about 3 times the volume of 70% alcohol for 12 hours, filter with filter paper and funnel, retain the filtrate and discard the filter residue; (3) place the obtained filtrate on a rotary evaporator for rotary evaporation, take it out when the filtrate is viscous and clings to the wall, and place it in a hot air sterilizer to evaporate the remaining alcohol.

3. The method for preparing the traditional Chinese medicine composition for treating gastric mucosal metaplasia according to claim 1, characterized in that, When the traditional Chinese medicine composition is in the form of powder, the following steps are included: take the above drug combination according to the weight parts, crush it, and pass it through a 10-mesh and a 50-mesh sieve at the same time. The coarse particles that cannot pass through the 10-mesh sieve should be crushed further, and the powder located between the 10-mesh and 50-mesh sieves should be taken; mix the raw material powders evenly, put them into a packaging bag, and seal it.

4. The method for preparing the traditional Chinese medicine composition for treating gastric mucosal metaplasia according to claim 1, characterized in that, When the traditional Chinese medicine composition is in the form of pills, the following steps are included: Take the above-mentioned combination of medicines according to the weight proportions, crush them, and pass them through 10-mesh and 50-mesh sieves. Continue to crush the coarse particles that cannot pass through the 10-mesh sieve. Take the powder that is between the 10-mesh and 50-mesh sieves and dry it in the sun. Take an appropriate amount of honey, simmer it slowly over low heat, stir, and skim off the impurities and foam on the surface. When the temperature rises to 116℃~119℃, add the medicine powder in a ratio of 1:1.2~1.5 and mix well. Roll the mixture into pills, each weighing about 3g. After the prepared honey pills have dried at room temperature, seal them in bags and store them separately.

5. The method for preparing the traditional Chinese medicine composition for treating gastric mucosal metaplasia according to claim 1, characterized in that, When the traditional Chinese medicine composition is a decoction, the following steps are included: weighing the raw materials according to the formula; soaking the raw materials in 8-12 times their weight of water for 30 minutes, then heating to boiling and maintaining a gentle boil, separating to obtain the first decoction and the residue; taking the residue obtained after the first decoction, adding 8-10 times the weight of water equivalent to the total weight of the raw materials, heating to boiling and maintaining a gentle boil, separating to obtain the second decoction and the residue; combining the first decoction and the second decoction to obtain an oral solution.

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