Preparation method and product of a health-care drink containing pachyman and rehmannia enzyme

Abstract

The application discloses a kind of poria cocos rehmannia enzyme health drinks preparation method and product, belong to poria cocos rehmannia health food field.The poria cocos rehmannia enzyme health drinks include the following raw materials according to weight parts: 45-55 portions of poria cocos, 45-55 portions of rehmannia glutinosa, 7-10 portions of red date, 5-6 portions of Chinese wolfberry and 18-22 portions of compound bacterial liquid;The compound bacterial liquid is made by mixing lactobacillus reuteri bacterial liquid and bifidobacterium adolescentis bacterial liquid according to volume ratio (1-1.5) :1;The preservation number of lactobacillus reuteri is CICC 6118, and the preservation number of bifidobacterium adolescentis is CICC 6178.Poria cocos and rehmannia are used as main raw materials, supplemented with a small amount of red date and Chinese wolfberry to enhance the taste and health care effect of the beverage, and the raw materials are safe and healthy, non-toxic and side effects;Synergistic effect between components, with the effects of improving intestinal tract, regulating intestinal flora and improving sleep.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of health-care food of poria cocos and rehmannia, in particular to a preparation method and product of a poria cocos and rehmannia enzyme health-care beverage. BACKGROUND

[0002] Enzyme is also called "enzyme". With the improvement of living standards, people's health awareness is gradually enhanced, and enzyme is more and more favored by people. Enzyme exists in all living cells, and is an essential substance for maintaining normal functions of the body and repairing tissues and other life activities. Enzyme is divided into two categories, i.e. external enzyme and internal enzyme. With the increase of age, the internal enzyme will gradually decrease, and the resistance of the human body will also decrease. At this time, external enzyme needs to be supplemented from the outside. There are two main ways to obtain external enzyme: one is raw food, but raw food is seasonally limited and cannot be freely supplemented and consumed, and pesticide pollution is serious, which will also cause the decrease of enzyme effect; the other is to take enzyme supplements.

[0003] Microbial enzyme is often produced by fermentation of one or more fresh vegetable parts, fruit parts, mushroom parts, Chinese herbal medicine and the like by a plurality of beneficial bacteria. The functional microbial fermentation product contains rich nutritional components such as vitamins, enzymes, minerals and secondary metabolites. The enzyme not only preserves the inherent nutrients in the fermentation raw materials, but also greatly increases the content of effective components, and can produce new bioactive components and biological enzymes such as superoxide anion free radicals, hydroxyl free radicals, DPPH free radicals, and active enzymes such as superoxide dismutase, amylase, protease and lipase. In medical care, enzyme can accelerate the metabolism of skin cells, promote the regeneration of dermal fibers, and secrete sebaceous glands and sweat glands, and has the characteristics of clear effect, strong specificity and good curative effect.

[0004] At present, the enzyme supplements on the market are mainly natural fermentation. The natural fermentation not only cannot make the effective components fully dissolved, resulting in waste of raw materials, but also is easy to cause pollution by other bacteria. In addition, the period of natural fermentation is long, generally 2-4 months, which cannot meet the market demand. Chinese patent 201210216324.X discloses a plant essence enzyme and a preparation method thereof. The plant essence enzyme is prepared by 4 times of oxygen increasing aeration culture of 20 kinds of fresh fruits including apples, 10 kinds of fresh vegetables including celery, sugar, trace elements, protein, wine, water and the like. The period of each oxygen increasing aeration culture is 10-18 days, and the production period is long. The existing technology generally uses several fresh fruits and vegetables as raw materials, and has the problems of single nutrition, insufficient effect and long production period.

[0005] Poria cocos and Rehmannia glutinosa are traditional Chinese medicinal materials, which have various health care effects, such as invigorating the spleen and soothing the heart, nourishing yin and tonifying the kidney. However, the traditional Chinese medicine taking mode is relatively cumbersome, and the taste is not good, which limits its popularization and application. Therefore, it is of great significance to develop a Poria cocos and Rehmannia glutinosa enzyme health care beverage which has good taste, is easy to take and has significant health care effect. At present, there are various health care beverages on the market, but there is no health care beverage prepared by effective extraction and reasonable collocation of Poria cocos and Rehmannia glutinosa and combined with enzyme fermentation process. SUMMARY

[0006] The purpose of the present application is to provide a Poria cocos and Rehmannia glutinosa enzyme health care beverage preparation method and product to solve the problems existing in the prior art. The Poria cocos and Rehmannia glutinosa enzyme health care beverage has synergistic effect between the components, and has the effects of improving the intestinal tract, regulating the intestinal flora and improving sleep.

[0007] To achieve the above-mentioned purpose, the present application provides the following solutions:

[0008] The present application provides a Poria cocos and Rehmannia glutinosa enzyme health care beverage, which comprises the following raw materials by weight:

[0009] Poria cocos 45-55 parts, Rehmannia glutinosa 45-55 parts, red dates 7-10 parts, wolfberry 5-6 parts and composite bacterial liquid 18-22 parts;

[0010] The composite bacterial liquid is prepared by mixing Lactobacillus reuteri bacterial liquid and Bifidobacterium adolescentis bacterial liquid at a volume ratio of (1-1.5) : 1;

[0011] The preservation number of the Lactobacillus reuteri is CICC 6118, and the preservation number of the Bifidobacterium adolescentis is CICC 6178.

[0012] Optionally, the following raw materials are included by weight: Poria cocos 50 parts, Rehmannia glutinosa 50 parts, red dates 10 parts, wolfberry 5 parts and composite bacterial liquid 20 parts.

[0013] Optionally, the viable bacterial count of the Lactobacillus reuteri bacterial liquid and the Bifidobacterium adolescentis bacterial liquid is 1.0x10 8 CFU / mL.

[0014] The present application also provides a preparation method of the Poria cocos and Rehmannia glutinosa enzyme health care beverage, comprising the following steps:

[0015] Mixing Lactobacillus reuteri bacterial liquid and Bifidobacterium adolescentis bacterial liquid at a volume ratio of (1-1.5) : 1 to obtain a composite bacterial liquid;

[0016] Taking Poria cocos, Rehmannia glutinosa, red dates and wolfberry by a specified weight, mixing and crushing to obtain a mixed powder;

[0017] The mixed powder is added with water in a weight ratio of 5-6 times of the total weight of the powder after sterilization, and heated for 2.5-3.5 hours for extraction, and then filtered to obtain an extract;

[0018] The extract is mixed with the compound bacterial solution, and then fermented and filtered to obtain the Poria cocos and Rehmannia glutinosa enzyme health-care beverage.

[0019] Optionally, the volume ratio of the Lactobacillus reuteri solution and Bifidobacterium adolescentis solution is 1.5:1.

[0020] Optionally, the sterilization is microwave sterilization at 85-95 DEG C for 100-120 seconds.

[0021] Optionally, the fermentation is anaerobic fermentation, and the fermentation temperature is 37 DEG C, and the fermentation time is 42-48 hours.

[0022] The application further provides the use of the Poria cocos and Rehmannia glutinosa enzyme health-care beverage in any of the following aspects:

[0023] In the regulation of intestinal flora,

[0024] In the preparation of health-care products for regulating intestinal flora,

[0025] In the improvement of sleep,

[0026] In the preparation of health-care products for improving sleep.

[0027] The application further provides a product for regulating intestinal flora and / or improving sleep, comprising the Poria cocos and Rehmannia glutinosa enzyme health-care beverage.

[0028] Optionally, the product comprises a health-care product.

[0029] The application discloses the following technical effects:

[0030] The application discloses a Poria cocos and Rehmannia glutinosa enzyme health-care beverage, which uses Poria cocos and Rehmannia glutinosa as main raw materials, and a small amount of red dates and wolfberry to enhance the taste and health-care effect of the beverage, and has the advantages of safe and healthy raw materials, no toxic side effects, no dependence effect, synergistic effect between components, and good effect.

[0031] In addition, the preparation method of the Poria cocos and Rehmannia glutinosa enzyme health-care beverage is simple, the raw materials are easy to obtain, the production operation is simple, the requirement for equipment is low, and the method can be used for large-scale industrial production. DETAILED DESCRIPTION

[0032] The following detailed description of various example embodiments of the application will not be considered limiting of the application, but rather as a description of certain aspects, features and embodiments of the application.

[0033] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. Additionally, for a range of values, the endpoints are included within the range. Any smaller range within a range of values is also contemplated. In lay terms, every possible combination of range endpoints is also contemplated.

[0034] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application. All documents mentioned herein are incorporated by reference to disclose and describe in further detail the methods and / or materials associated with the documents. In the case of conflict between the present specification and any document incorporated by reference, the present specification will control.

[0035] Many modifications and variations of this application can be made in the light of the above teachings without departing from the spirit and scope thereof, and it is to be understood that all such modifications and variations warrant the patentable subject matter under the patent laws. Other embodiments of the application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. The specification and examples given are exemplary only.

[0036] As used herein, the terms "comprises", "comprising", "includes", "including", "has", "having", "contains", "containing", or variations thereof, are intended to be open-ended terms that mean including, but not limited to.

[0037] The Lactobacillus reuteri used in the present application has the preservation number of CICC 6118, and the Bifidobacterium adolensentis used in the present application has the preservation number of CICC 6178, both of which are purchased from the China Industrial Microbial Culture Collection Center.

[0038] NA medium: 10.0 g of proteose peptone, 3.0 g of beef extract, 5.0 g of NaCl, 15.0 g of agar and 1000 mL of distilled water are adjusted to pH 7.0, sterilized, and cooled for standby.

[0039] Seed liquid medium: casein peptone (pancreatic digestion) 10.0 g, beef extract 10.0 g, yeast extract 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, agar 15.0 g and distilled water 1000 mL, adjust pH to 6.3, sterilize, cool and reserve.

[0040] Raw material selection: high-quality Poria cocos and Rehmannia glutinosa were selected as main raw materials, which were strictly screened and tested to ensure that they were free of impurities, moldy, insect-infested, and had good medicinal value. At the same time, an appropriate amount of auxiliary materials (red dates, Chinese wolfberry) were matched to enhance the taste and health care effect of the beverage. Before operation, the raw materials such as Poria cocos and Rehmannia glutinosa were washed to remove the surface dirt and impurities. Then, the raw materials were cut into appropriate size blocks or slices.

[0041] Example 1: A Poria cocos and Rehmannia glutinosa enzyme health care beverage

[0042] The raw materials include the following components by weight: Poria cocos 50 parts, Rehmannia glutinosa 50 parts, red dates 10 parts, Chinese wolfberry 5 parts and composite bacterial liquid 20 parts.

[0043] The preparation method is as follows:

[0044] Preparation of composite bacterial liquid: Lactobacillus reuteri and Bifidobacterium adolescentis were inoculated into NA medium respectively, and cultured anaerobically at 37°C for 24h to obtain activated strains;

[0045] Then, under sterile conditions, the activated strains were inoculated into 250mL conical flasks preloaded with 100mL seed liquid medium at a proportion of 1wt%, and then cultured in a constant temperature shaker at 37°C and 180rpm for 48h. The standard curve of linear relationship between cell concentration and OD 600 value was established by plate counting method combined with turbidity method. After multiple test verification, the OD 600 value of each bacterial liquid could exceed 2.0 after 48h under the above activation conditions, and the effective viable bacterial count of each bacterial liquid could reach more than 1.0×10 8 CFU / mL.

[0046] The Lactobacillus reuteri bacterial liquid and Bifidobacterium adolescentis bacterial liquid were uniformly mixed at a volume ratio of 1.5:1 to obtain the composite bacterial liquid.

[0047] Preparation of extract: Poria cocos, Rehmannia glutinosa, red dates and Chinese wolfberry were mixed according to the specified weight, crushed by a traditional Chinese medicine crusher, sieved through an 80 mesh sieve, and the mixed powder was sterilized by microwave at 85°C for 120s; 5 times the weight of water was added, heated to boiling, extracted for 3 hours, filtered to obtain the extract.

[0048] Fermentation: the extract liquid is mixed with the compound bacteria liquid, and after being sealed, anaerobic fermentation is carried out in a fermentation tank, the fermentation temperature is 37℃, and the fermentation time is 42h.

[0049] The fermented product after fermentation is completed is filtered by a bag filter, and a Fuling Dihuang enzyme health drink is obtained.

[0050] Example 2: A Fuling Dihuang enzyme health drink

[0051] The raw materials include the following components by weight: 45 parts of Fuling, 55 parts of Dihuang, 8 parts of red dates, 6 parts of wolfberry, and 18 parts of compound bacteria liquid.

[0052] The preparation method is as follows:

[0053] Preparation of compound bacteria liquid: Lactobacillus reuteri and Bifidobacterium adolescentis are inoculated into NA culture medium respectively, and anaerobic culture is carried out at 37℃ for 24h to obtain activated bacteria of each strain;

[0054] Then under sterile conditions, the activated bacteria of each strain are inoculated into a 250mL conical flask preloaded with 100mL seed liquid culture medium at a proportion of 1wt%, and then shaken and cultured at 37℃ and 180rpm on a constant temperature shaker for 48h. The standard curve of linear relationship between cell concentration and OD 600 value of each strain is established by plate counting method combined with turbidity method. After multiple test verification, the OD 600 value of each strain liquid can exceed 2.0 after 48h under the above activation conditions, and at this time the effective viable cell count of each bacteria liquid can reach 1.0×10 8 CFU / mL or more.

[0055] The Lactobacillus reuteri liquid and the Bifidobacterium adolescentis liquid are uniformly mixed at a volume ratio of 1.5:1 to obtain the compound bacteria liquid.

[0056] Preparation of extract liquid: Fuling, Dihuang, red dates and wolfberry are mixed according to the specified weight, crushed by a traditional Chinese medicine crusher, sieved through an 80 mesh sieve, and the mixed powder is sterilized by microwave at 90℃ for 100s; 6 times the weight of water based on the total weight is added, heated to boiling, extracted for 3.5 hours, filtered, and the extract liquid is obtained.

[0057] Fermentation: the extract liquid is mixed with the compound bacteria liquid, and after being sealed, anaerobic fermentation is carried out in a fermentation tank, the fermentation temperature is 37℃, and the fermentation time is 48h.

[0058] The fermented product after fermentation is completed is filtered by a bag filter, and a Fuling Dihuang enzyme health drink is obtained.

[0059] Example 3: A Fuling Dihuang enzyme health drink

[0060] The raw materials include the following components by weight: 55 parts of poria cocos, 45 parts of rehmannia, 7 parts of red dates, 6 parts of wolfberry, and 22 parts of complex bacterial liquid.

[0061] The preparation method is as follows:

[0062] The complex bacterial liquid is prepared: Lactobacillus reuteri and Bifidobacterium adolescentis are inoculated into NA culture medium respectively, and are cultured anaerobically at 37°C for 24 hours to obtain activated strains;

[0063] Then, under sterile conditions, the activated strains are inoculated into 250 mL conical flasks pre-loaded with 100 mL seed liquid culture medium at a proportion of 1 wt%, and then are cultured on a constant temperature shaker at 37°C and 180 rpm for 48 hours. Plate counting method combined with turbidity method is used to establish a standard curve of linear relationship between cell concentration and OD 600 value of each strain. After repeated test verification, the OD 600 value of each strain liquid can exceed 2.0 after 48 hours according to the above activation conditions, and at this time the effective viable cell count of each liquid can reach more than 1.0×10 8 CFU / mL.

[0064] The Lactobacillus reuteri liquid and the Bifidobacterium adolescentis liquid are uniformly mixed at a volume ratio of 1:1 to obtain the complex bacterial liquid.

[0065] The extraction liquid is prepared: Poria cocos, rehmannia, red dates, and wolfberry are mixed according to the specified weight parts, are crushed by a traditional Chinese medicine crushing machine, are sieved through an 80-mesh sieve, and the mixed powder is sterilized by microwave at 95°C for 100 seconds; 5 times the weight of water based on the total weight is added, is heated to boiling, is extracted for 2.5 hours, is filtered, and the extraction liquid is obtained.

[0066] Fermentation: The extraction liquid and the complex bacterial liquid are mixed, are sealed, and are subjected to anaerobic fermentation in a fermentation tank at a fermentation temperature of 37°C for 45 hours.

[0067] The fermented product after fermentation is filtered by a bag filter to obtain a poria cocos-rehmannia enzyme health drink.

[0068] Comparative Example 1: A poria cocos-rehmannia enzyme health drink

[0069] The same as Example 1, except that the raw materials do not include red dates and wolfberry. Specifically, the raw materials include the following components by weight: 50 parts of poria cocos, 50 parts of rehmannia, and 20 parts of complex bacterial liquid.

[0070] The preparation method is the same as that of Example 1.

[0071] Comparative Example 2: A poria cocos-rehmannia enzyme health drink

[0072] The same as example 1, the only difference is that the complex bacteria liquid is not used, only lactobacillus reuteri single bacteria liquid (effective viable count is 1.0 x 10 8 CFU / mL) is used.

[0073] Comparative example 3 A health-care drink of poria cocos and rehmannia glutinosa enzyme

[0074] The same as example 1, the only difference is that the complex bacteria liquid is not used, only lactobacillus reuteri single bacteria liquid (effective viable count is 1.0 x 10 8 CFU / mL) is used.

[0075] Application example Intestinal tract performance test of mice

[0076] Select ordinary Kunming white mice, half male and half female, 18-20g, and feed normally. Randomly select 70 from them, and give them intragastric administration of lincomycin hydrochloride 0.2mL (20mg) per mouse at 9:00 every morning. Select another 10 as a blank control group, and give them intragastric administration of the same amount of sterilized normal saline at the same time every day, continuously for one week, to prepare a mouse model of intestinal flora imbalance. The model group of mice has decreased diet, no death and obvious diarrhea phenomenon, soft feces, normal appearance, more water content, and damp bedding. Divide the 70 mice of intestinal flora imbalance into 7 groups at random, of which 6 groups are given intragastric administration of the health-care drink of poria cocos and rehmannia glutinosa enzyme prepared in examples 1-3 and comparative examples 1-3 at 10mL / kg per day once a day, and the other group is taken as a natural recovery group, and the blank control group is given intragastric administration of the same amount of sterilized normal saline at the same time every day, continuously for 30 days. Observe the growth and defecation of the white mice every day for 37 days, weigh the mice in each group on the 8th day and the 37th day, and calculate the average weight growth rate of each group.

[0077] Measure the number of fecal E. coli of the model group and the health-care drink of poria cocos and rehmannia glutinosa enzyme intervention group of mice every 5 days, and calculate the average number.

[0078] As shown in table 1, the average weight growth rate of the model mice using the health-care drink of poria cocos and rehmannia glutinosa enzyme prepared in examples 1-3 and comparative examples 1-3 is significantly higher than that of the natural recovery group, and it can be seen that the health-care drink of poria cocos and rehmannia glutinosa enzyme prepared by the application has the effect of improving the intestinal tract. At the same time, the effect of examples 1-3 is significantly better than that of comparative examples 1-3, and the average growth rate of examples 1-3 can be flat with the blank control group. It shows that comparative examples 1-3 lack the coordination of auxiliary materials (jujube and wolfberry) or use single bacteria liquid, and the health-care effect is far from that of examples 1-3.

[0079] Take about 0.1g of mouse feces, add 3 glass beads (0.1g of fecal sample + 0.5mL of diluent) in a sterile operation table, dilute and inoculate McConkey agar medium, and calculate the number of E. coli in each gram of wet feces.

[0080] The results are shown in Table 2. After the intervention of the Poria cocos and Rehmannia glutinosa enzyme health-care beverage, the number of E. coli in the intestinal tract was significantly reduced, which was significantly lower than that of the natural recovery group. It can be seen that the Poria cocos and Rehmannia glutinosa enzyme health-care beverage prepared in the application has the effect of regulating intestinal flora. At the same time, the effects of the example 1-3 groups are significantly better than those of the comparative example 1-3 groups, indicating that the health-care effects of the comparative example 1-3 are far from those of the example 1-3 due to the lack of the coordination of the auxiliary materials (jujube and wolfberry) or the use of a single bacterial liquid.

[0081] In summary of the above results, the Poria cocos and Rehmannia glutinosa enzyme health-care beverage prepared in the application has the effects of improving the intestinal tract and regulating intestinal flora. The probiotics in the health-care beverage can rapidly colonize in the intestinal tract of the mice, form a dominant flora, and effectively inhibit the growth and reproduction of pathogenic bacteria such as E. coli, and the colonization time is long, which continuously and effectively improves the intestinal performance.

[0082] Table 1: Effect on the body weight of mice

[0083] Group Average initial weight / g Average final weight / g Average growth rate / % Blank control group 25.36±1.42 49.28±1.48 94.32 b ]] Natural recovery group 20.58±1.24 27.38±1.66 33.04 a ]]> Example 1 group 20.39±1.18 39.17±1.37 92.10 b ]]> Example 2 group 20.64±1.27 38.98±1.40 88.86 b ]]> Example 3 group 20.48±1.15 38.81±1.35 89.50 b ]] Comparative example 1 group 20.54±1.30 30.14±1.59 46.74 c ]]> Comparative example 2 group 20.53±1.28 29.65±1.63 44.42 c ]]> Comparative example 3 group 20.41±1.20 29.14±1.65 42.77 c ]]>

[0084] Note: Different superscripts indicate that the difference is statistically significant (P<0.05).

[0085] Table 2: Effect on the fecal E. coli of mice

[0086] Group Initial (x10 5 cfu / g) End (x10 5 cfu / g) Average decrease rate / % Natural recovery group 5.79±0.64 4.61±1.66 20.38 a ]]> Example 1 group 5.75±0.19 0.90±1.40 84.38 b ]] Example 2 group 5.82±0.28 0.92±1.37 84.19 b ]]> Example 3 group 5.77±0.25 0.92±1.35 84.06 b ]] Comparative example 1 group 5.76±0.27 2.49±1.59 56.77 c ]] Comparative example 2 group 5.80±0.20 2.66±1.63 54.14 c ]]> Comparative example 3 group 5.78±0.22 2.71±1.65 53.11 c ]]

[0087] Note: Different superscripts indicate that the difference is statistically significant (P<0.05).

[0088] It is also found in the experimental process that the sleep duration of the mice in the group intervened with the Poria cocos and Rehmannia glutinosa enzyme health-care beverage is higher than that of the natural recovery group, which is presumed that the Poria cocos and Rehmannia glutinosa enzyme health-care beverage prepared in the application has the effect of improving sleep.

[0089] Application Example 2: Effect test of soothing the nerves and helping sleep

[0090] Research object: 140 volunteers with different degrees of insomnia symptoms were selected for the test, aged 20-60 years old, including 70 males and 70 females. The volunteers were randomly divided into 7 groups: example 1-3 groups, comparative example 1-3 groups, and a blank group, 20 people in each group.

[0091] The volunteers met one or more of the following symptoms within the last 3 months before starting the test:

[0092] 1. For at least 3 days a week for a month, the sleep time was more than 30 minutes;

[0093] 2. The actual sleep time per day was less than 6.5 hours (regular work and rest, no bad habits before sleep);

[0094] 3. The number of times of waking up at night during sleep is more than 2, it is difficult to fall asleep after waking up, and the time of waking up is more than 30 minutes per night;

[0095] 4. During sleep, it is easy to be in light sleep, easy to wake up, and easy to have dreams and nightmares;

[0096] 5. The next day after getting up, it is accompanied by drowsiness, fatigue, poor mental state, and decreased cognitive function.

[0097] Test scheme: each test group drinks the corresponding example or the prepared Fuling Dihuang enzyme health care beverage of the comparative example: twice a day, 200 mL each time, continuously for 10 days; the blank group drinks the same amount of white water every day. The volunteers have no bad habits, and they eat light and have regular work and rest during the experiment. After 10 days, the number of improved and not improved in each test group is counted, and the effective rate is calculated (effective rate = (number of improved / total number x 100%).

[0098] The test results are shown in Table 3, and the sleep can be significantly improved by drinking the Fuling Dihuang enzyme beverage prepared by the present application, wherein the improvement effect of the example 1-3 group is significantly better than that of the comparative example 1-3 group, and it is speculated that the comparative example 1-3 lacks the coordination of auxiliary materials (red dates and medlar) or uses single bacterial liquid, and its improvement effect on insomnia is far less than that of the example 1-3.

[0099] Table 3: Effect of Fuling Dihuang enzyme health care beverage on soothing the nerves and improving sleep

[0100] Group Number of people improved Number of people not improved Efficiency / % Blank group 1 19 5 a ]] Example 1 group 17 3 85 b ]] Example 2 group 16 4 80 b ]] Example 3 group 17 3 85 b ]] Comparative example 1 group 12 8 60 c ]] Comparative example 2 group 12 8 60 c ]] Comparative example 3 group 11 9 55 c ]]

[0101] Note: Different shoulder letters indicate that the difference is statistically significant (P<0.05).

[0102] After 2 months of the test, the volunteers who drink the Fuling Dihuang enzyme health care beverage are randomly visited, the volunteers feedback that the symptoms of insomnia have improved, no adverse reactions have occurred during the drinking period, and no side effects have occurred after not drinking the Fuling Dihuang enzyme health care beverage.

[0103] The above examples only describe the preferred mode of the present application, and do not limit the scope of the present application, and various modifications and improvements of the technical solutions of the present application made by those skilled in the art without departing from the design spirit of the present application shall fall within the protection scope determined by the claims of the present application.

Claims

1. The use of Poria cocos and Rehmannia glutinosa ferment health care beverage in the preparation of products for regulating intestinal flora and improving sleep, characterized in that, The Poria cocos and Rehmannia glutinosa ferment health-care beverage is made of the following raw materials by weight parts: Poria cocos 45-55 parts, Rehmannia glutinosa 45-55 parts, red dates 7-10 parts, Chinese wolfberry 5-6 parts and compound bacterial liquid 18-22 parts; The compound bacterial liquid is made by mixing Lactobacillus reuteri bacterial liquid and Bifidobacterium adolescentis bacterial liquid at a volume ratio of (1-1.5):1; The preservation number of the Lactobacillus reuteri is CICC 6118, and the preservation number of the Bifidobacterium adolescentis is CICC 6178; The viable cell count of the Lactobacillus reuteri bacterial solution and the Bifidobacterium adolescentis bacterial solution is 1.0 x 10 8 CFU / mL; The preparation method of the Poria cocos and Rehmannia glutinosa ferment health-care beverage comprises the following steps: Mixing Lactobacillus reuteri bacterial liquid and Bifidobacterium adolescentis bacterial liquid at a volume ratio of (1-1.5):1 to obtain a compound bacterial liquid; Taking Poria cocos, Rehmannia glutinosa, red dates and Chinese wolfberry by the specified weight parts, mixing and crushing to obtain a mixed powder; After sterilizing the mixed powder, adding water with a weight of 5-6 times of the total weight, heating and extracting for 2.5-3.5 hours, filtering to obtain an extract; Mixing the extract and the compound bacterial liquid, fermenting and filtering to obtain the Poria cocos and Rehmannia glutinosa ferment health-care beverage.

2. Use according to claim 1, wherein The Poria cocos and Rehmannia glutinosa ferment health-care beverage is made of the following raw materials by weight parts: Poria cocos 50 parts, Rehmannia glutinosa 50 parts, red dates 10 parts, Chinese wolfberry 5 parts and compound bacterial liquid 20 parts.

3. The use according to claim 1, wherein The volume ratio of the Lactobacillus reuteri bacterial liquid and the Bifidobacterium adolescentis bacterial liquid is 1.5:

1.

4. The use according to claim 1, wherein The sterilization is 85-95℃ microwave sterilization for 100-120s.

5. The use according to claim 1, wherein The fermentation condition is anaerobic fermentation, the fermentation temperature is 37℃, and the fermentation time is 42-48h.

6. A product for regulating intestinal flora and improving sleep, characterized by, The Poria cocos and Rehmannia glutinosa ferment health-care beverage as claimed in claim 1.

7. The product of claim 6, wherein, The product comprises health-care products.