Fermented composition for improving lung ventilation function and method of preparation

Abstract

The application provides a fermented composition for moistening and nourishing the lung and a preparation method. The fermented composition comprises the following components in parts by mass: 20-60 parts of yam, 15-40 parts of dark plum, 10-30 parts of polygonatum, 10-30 parts of ginkgo, 5-25 parts of raphanus, 0.3-3 parts of fermented bacteria powder and 3.1-13 parts of enzyme preparation. The fermented composition with the function of moistening and nourishing the lung is prepared by the joint action of the fermented bacteria powder and the enzyme preparation on the five main components of the food and medicine.

Description

Technical Field

[0001] This invention belongs to the field of fermentation preparation technology, specifically relating to a fermentation composition for improving lung ventilation function, and a method for preparing the fermentation composition. Background Technology

[0002] Traditional Chinese medicine believes that "the lungs are a delicate organ, located at the highest point, and are intolerant of cold and heat." Furthermore, the lungs "prefer moisture and dislike dryness," making them most susceptible to damage from dryness.

[0003] Currently available lung-clearing tablets and teas only provide a one-sided effect of clearing lung heat. Traditional Chinese medicine fermentation technology, on the other hand, is a technology that can better protect the active ingredients of traditional Chinese medicine from damage, improve the extraction rate of effective components, degrade macromolecular substances in traditional Chinese medicine, generate new active substances, enhance the function of probiotics during fermentation, and reduce the toxic side effects of traditional Chinese medicine. Summary of the Invention

[0004] In view of this, the present invention combines traditional Chinese medicine fermentation technology to provide an effective fermentation composition that can significantly improve lung ventilation function, thereby effectively relieving symptoms such as difficulty breathing, shortness of breath, and dyspnea.

[0005] Another object of the present invention is to provide a method for preparing the composition. This method is simple and suitable for industrial production.

[0006] Furthermore, the purpose of this invention is to provide a product that improves lung ventilation function, effectively relieves symptoms such as difficulty breathing and shortness of breath, and has a good taste.

[0007] The present invention achieves the above objectives using the following technical solutions.

[0008] A fermented composition for improving lung ventilation function, comprising, by weight, 20-60 parts of yam, 15-40 parts of dried plum, 10-30 parts of Solomon's seal rhizome, 10-30 parts of ginkgo, 5-25 parts of radish seed, 0.3-3 parts of fermentation starter culture powder, and 3.1-13 parts of enzyme preparation.

[0009] Preferably, the fermentation powder includes 0.05-0.5 parts of *Lactobacillus plantarum*, 0.1-0.9 parts of *Bifidobacterium longum* subsp. *longum*, 0.1-0.6 parts of *Lactococcus lactis* subsp. *lactolaccos*, and 0.1-0.9 parts of *Lactobacillus fermentum*.

[0010] More preferably, the weight ratio of *Lactobacillus plantarum*, *Bifidobacterium longum* subsp. *longum*, *Lactococcus lactis* subsp. *lactococcus*, and *Lactobacillus fermentum* is 1:3:2:3.

[0011] Preferably, the enzyme preparation comprises 1-5 parts cellulase, 2-6 parts pectinase, and 0.1-2 parts acidic protease.

[0012] More preferably, the weight ratio of cellulase, pectinase and acidic protease is 2.2:2.2:1.

[0013] More preferably, the bacterial activity of *Lactobacillus plantarum* is 100-300 billion CFU / g, the bacterial activity of *Bifidobacterium longum* subsp. longum is 100-400 billion CFU / g, the bacterial activity of *Lactococcus lactis* subsp. lactis is 80-300 billion CFU / g, and the bacterial activity of *Lactobacillus fermentum* is 50-200 billion CFU / g.

[0014] More preferably, the cellulase has an enzyme activity of 10,000-28,000 U / g, the pectinase has an enzyme activity of 5,000-40,000 U / g, and the acidic protease has an enzyme activity of 5,000-20,000 U / g.

[0015] A method for preparing the above-mentioned fermentation composition includes the following steps: (1) crushing yam, dried plum, Solomon's seal, ginkgo and radish seeds, sieving to a mesh size of 100-200, adding water and mixing evenly, and adding an acid-base adjuster to a pH of 4.0-5.0 to obtain a mixed solution; (2) adding an enzyme preparation to the mixed solution for enzymatic hydrolysis, and adding an acid-base adjuster to a pH of 5.0-6.0 after the enzymatic hydrolysis is completed to obtain an enzymatic hydrolysate; (3) extracting the enzymatic hydrolysate to obtain an extract; (4) adding fermentation bacteria powder to the extract for fermentation to obtain a fermentation broth, and separating the fermentation broth into solid and liquid components to obtain a clear fermentation broth.

[0016] Preferably, the enzymatic hydrolysis conditions in step (2) are a temperature of 35-65℃ and a time of 100-260 min; the extraction conditions in step (3) are a temperature of 75-110℃ and a time of 15-90 min; the fermentation temperature in step (4) is 25-42℃ and the time is 48-96 h; and the acid-base regulator is sodium citrate.

[0017] A product for improving lung ventilation function, comprising the above-mentioned fermented composition for improving lung ventilation function.

[0018] Preferably, a product for improving lung ventilation also includes flavoring agents and / or sweeteners.

[0019] More preferably, the flavoring agent is selected from one or more of apple powder, pear powder, orange powder, loquat powder, or pomegranate powder.

[0020] The sweetener is further preferably selected from one or more of erythritol, xylitol, sorbitol, xylooligosaccharides, fructooligosaccharides, or inulin.

[0021] More preferably, the flavoring agent comprises 8-10 parts apple powder, 10-15 parts pear powder and 12-18 parts loquat powder; the sweetener comprises 18-25 parts erythritol and 40-50 parts fructooligosaccharides.

[0022] A method for preparing the above-mentioned product for improving pulmonary ventilation function includes the following steps:

[0023] (1) Crush yam, dried plum, Solomon's seal, ginkgo and radish seed, sieve to 100-200 mesh, add water and mix evenly, then add acid-base adjuster to pH 4.0-5.0 to obtain a mixed solution; (2) Add enzyme preparation to the mixed solution for enzymatic hydrolysis, and after the enzymatic hydrolysis is completed, add acid-base adjuster to pH 5.0-6.0 to obtain an enzymatic hydrolysate; (3) Extract the enzymatic hydrolysate to obtain an extract; (4) Add fermentation liquid powder to the extract for fermentation to obtain a fermentation liquid, and separate the solid and liquid of the fermentation liquid to obtain a fermentation clear liquid; (5) Add flavoring agent and / or sweetener to the fermentation clear liquid and mix evenly to obtain the final product.

[0024] More preferably, the enzymatic hydrolysis conditions in step (2) are a temperature of 35-65℃ and a time of 100-260 min; the extraction temperature in step (3) is 75-110℃ and the time is 15-90 min; the fermentation temperature in step (4) is 25-42℃ and the time is 48-96 h; and the acid-base regulator is sodium citrate.

[0025] The technical solution of this invention has the following advantages:

[0026] 1. Yam: Sweet in taste and neutral in nature; it enters the spleen, lung, and kidney meridians. It benefits qi and nourishes yin, tonifies the spleen, lungs, and kidneys, and strengthens essence and stops leukorrhea. The *Compendium of Materia Medica* states: It benefits kidney qi, strengthens the spleen and stomach, stops diarrhea, resolves phlegm, and moisturizes the skin and hair. Yam is rich in saponins and mucilage, all of which have excellent lung-benefiting and lung-nourishing effects, which is what traditional Chinese medicine considers as benefiting lung qi and nourishing lung yin, thus helping to treat lung deficiency with phlegm and cough. *Medical Records of Integrating Chinese and Western Medicine* records: "Yam is white and enters the lung meridian; its sweet taste enters the spleen meridian; its thick liquid benefits the kidneys; it can nourish blood vessels, astringe qi, calm cough and asthma, strengthen will and nourish spirit; its neutral nature allows for regular and frequent consumption." It can tonify lung qi and improve chronic cough due to lung deficiency, and consumptive cough.

[0027] Ume plum: Sour and astringent in taste, neutral in nature; enters the liver, spleen, lung, and large intestine meridians. It astringes the lungs to stop coughing, astringes the intestines to stop diarrhea, expels roundworms and relieves pain, and promotes the production of body fluids to quench thirst. Rich in vitamins B and C, as well as minerals, it can nourish the lungs and enhance their resistance.

[0028] Polygonatum odoratum: sweet in taste and neutral in nature; it enters the lung and stomach meridians. It nourishes yin and moistens dryness, promotes body fluid and quenches thirst. Polygonatum odoratum contains convalescent glycoside, convalescent glycoside, kaempferol, quercetin glycoside, vitamin A, alkaloids, Polygonatum odoratum polysaccharides and four kinds of Polygonatum odoratum fructans, etc., which can nourish yin and moisten the lungs, calm the mind and soothe the nerves, and improve sleep.

[0029] Ginkgo seed: sweet, bitter, astringent, neutral in nature; attributing to the lung and kidney meridians. Astringing the lung qi, calming panting, arresting vaginal discharge, stopping diarrhea, detoxifying, reducing frequent urination.

[0030] Semen Raphani: pungent, sweet, neutral in nature; attributing to the lung, spleen and stomach meridians. Promoting digestion to relieve distension, lowering qi to resolve phlegm. Modern pharmacological research shows that the chemical constituents of Semen Raphani mainly include alkaloids, sulfur glycosides, volatile components, fatty acids, flavonoids, etc., and have pharmacological activities such as enhancing gastrointestinal motility, lowering blood pressure, lowering blood lipids, anti-cancer, resolving phlegm, relieving cough and calming panting.

[0031] The present invention selects the above 5 kinds of medicine and food homologous raw materials as the main components. Under the action of enzyme preparations and strains, the fermented product prepared by the synergistic cooperation of each raw material can significantly improve the pulmonary ventilation function, thereby effectively relieving symptoms such as dyspnea and shortness of breath.

[0032] 2. The composition for improving pulmonary ventilation function provided by the present invention is enzymatically hydrolyzed with cellulase, pectinase and acidic protease and then fermented by Lactiplantibacillus plantarum, Bifidobacterium longum subsp. longum, Lactococcus lactis subsp. lactis and Lactobacillus mucosae fermentans, decomposing the macromolecular substances of traditional Chinese medicine into small molecules, increasing the effective active ingredients in traditional Chinese medicine and being more conducive to absorption.

[0033] 3. For the fermented composition for improving pulmonary ventilation function provided by the present invention, the acid-base regulator can adjust the pH value during the fermentation process, be more suitable for the proliferation and vitality of strains, and improve the fermentation yield.

[0034] 4. The fermented composition for improving pulmonary ventilation function provided by the present invention can be used as a basic raw material and prepared into different dosage forms such as liquid, ointment, powder, etc. Detailed implementation mode

[0035] To make the purpose, technical solutions and advantages of the present invention clearer, the following will further describe the implementation modes of the present invention in detail. Based on the embodiments in the present disclosure, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of this disclosure.

[0036] For those not specifying specific experimental steps or conditions in the examples, the operations or conditions of the conventional steps described in the literature in this field can be followed. For all reagents not indicating the manufacturer, they are all conventional reagent products that can be obtained through commercial purchase.

[0037] In the following examples, the source information of some products is as follows:

[0038] Chinese yam is the dried rhizome of Dioscorea opposita Thunb. of the Dioscoreaceae family, purchased from Bozhou Shishi Pharmaceutical Co., Ltd.

[0039] Black plum is the dried flesh of Prunus mume Sieb. et Zucc. of the Rosaceae family, purchased from Bozhou Shishi Pharmaceutical Co., Ltd.

[0040] Polygonatum odoratum is the dried rhizome of Polygonatum odoratum, a plant belonging to the genus Polygonatum in the family Liliaceae. It was purchased from Anhui Jiahe Traditional Chinese Medicine Technology Co., Ltd.

[0041] Ginkgo seeds are the dried, mature seeds of Ginkgo biloba, a plant in the Ginkgoaceae family, purchased from Bozhou Guhongtang Pharmaceutical Co., Ltd.

[0042] Radish seeds are the dried seeds of the radish plant (Radish spp.), belonging to the Brassicaceae family. They were purchased from Shishi Pharmaceutical Co., Ltd. in Haozhou City.

[0043] Lactobacillus plantarum LP-A3 was purchased from Shandong Zhongke Jiayi Biotechnology Co., Ltd.

[0044] Bifidobacterium longum subsp. JBLC-141 was purchased from Shandong Zhongke Jiayi Biotechnology Co., Ltd.

[0045] Lactococcus lactis subsp. JYLL-60 was purchased from Shandong Zhongke Jiayi Biotechnology Co., Ltd.

[0046] Lactobacillus fermentum strain suo was purchased from Jiangsu Xin Shen Ao Biotechnology Co., Ltd.

[0047] Cellulase, pectinase, and acidic protease were purchased from Shandong Longkete Enzyme Preparation Co., Ltd.

[0048] Example 1

[0049] This embodiment provides a fermented composition for improving lung ventilation, wherein the total weight of yam, dried plum, Solomon's seal, ginkgo, and radish seed is 100g, specifically comprising 35 parts yam, 20 parts dried plum, 15 parts Solomon's seal, 15 parts ginkgo, 10 parts radish seed, 0.1 parts *Lactobacillus plantarum*, 0.3 parts *Bifidobacterium longum* subsp. *longum*, 0.2 parts *Lactococcus lactis* subsp. *lactolaccos*, 0.3 parts *Lactobacillus fermentum*, 2.2 parts cellulase, 2.2 parts pectinase, 1 part acidic protease, 8.5 parts sodium citrate, and 2000 parts water. The preparation method is as follows:

[0050] (1) 35 parts of yam, 20 parts of dried plum, 15 parts of Solomon's seal, 15 parts of ginkgo and 10 parts of radish seed were crushed and passed through a 200-mesh sieve. They were then mixed with 2000 parts of water and 4.5 parts of sodium citrate were added to adjust the pH to 4.7 to obtain a mixed solution.

[0051] (2) Add 2.2 parts of cellulase, 2.2 parts of pectinase, and 1 part of acidic protease to the mixture for enzymatic hydrolysis. Hydrolyze at 58℃ for 150 min. After hydrolysis, add 4 parts of sodium citrate to adjust the pH to 5.6 to obtain the hydrolysate. The cellulase activity is 18000 U / g, the pectinase activity is 23000 U / g, and the acidic protease activity is 12000 U / g.

[0052] (3) Heat the enzymatic hydrolysate to 95°C and stir for 30 min to obtain the extract;

[0053] (4) After the temperature drops to 35℃, add 0.1 parts of Lactobacillus plantarum, 0.3 parts of Bifidobacterium longum subsp. longum, 0.2 parts of Lactococcus lactis subsp. lactic acid, and 0.3 parts of Lactobacillus fermentum to the extract. Ferment at 35℃ for 72 hours to obtain the fermentation broth; wherein the viable count of Lactobacillus plantarum is 120 billion CFU / g, Bifidobacterium longum subsp. longum is 100 billion CFU / g, Lactococcus lactis subsp. lactic acid is 220 billion CFU / g, and Lactobacillus fermentum subsp. lactic acid is 100 billion CFU / g.

[0054] (5) Separate the solid and liquid of the above fermentation broth to obtain a clear fermentation broth with a pH of 3.4, a soluble solids content of 9.6%, and a total acid content of 1.33% (calculated as lactic acid).

[0055] Example 2

[0056] This embodiment provides a fermented composition for improving lung ventilation, wherein the total weight of yam, dried plum, Solomon's seal, ginkgo, and radish seed is 100g, specifically comprising 20 parts yam, 40 parts dried plum, 10 parts Solomon's seal, 30 parts ginkgo, 5 parts radish seed, 0.3 parts *Lactobacillus plantarum*, 0.9 parts *Bifidobacterium longum* subsp. *longum*, 0.6 parts *Lactococcus lactis* subsp. *lactolaccos*, 0.9 parts *Lactobacillus fermentum*, 5 parts cellulase, 2 parts pectinase, 0.1 parts acidic protease, 9 parts sodium citrate, and 2000 parts water. The preparation method is as follows:

[0057] (1) 20 parts of yam, 40 parts of dried plum, 10 parts of Solomon's seal, 30 parts of ginkgo and 5 parts of radish seed were crushed and passed through a 200-mesh sieve. They were then mixed with 2000 parts of water and 5 parts of sodium citrate were added to adjust the pH to 4.7 to obtain a mixed solution.

[0058] (2) Add 5 parts of cellulase, 2 parts of pectinase, and 0.1 parts of acidic protease to the mixture for enzymatic hydrolysis. Hydrolyze at 50℃ for 180 min. After hydrolysis, add 4 parts of sodium citrate to adjust the pH to 6.0 to obtain the hydrolysate. The cellulase activity is 18000 U / g, the pectinase activity is 23000 U / g, and the acidic protease activity is 12000 U / g.

[0059] (3) Heat the enzymatic hydrolysate to 80°C and stir for 60 min to obtain the extract;

[0060] (4) When the temperature drops to 30℃, add 0.3 parts of Lactobacillus plantarum, 0.9 parts of Bifidobacterium longum subsp. longum, 0.6 parts of Lactococcus lactis subsp. lactic acid, and 0.9 parts of Lactobacillus fermentum to the extract. Ferment at 40℃ for 80 hours to obtain fermentation broth; wherein the viable count of Lactobacillus plantarum is 120 billion CFU / g, Bifidobacterium longum subsp. longum is 100 billion CFU / g, Lactococcus lactis subsp. lactic acid is 220 billion CFU / g, and Lactobacillus fermentum subsp. lactic acid is 100 billion CFU / g.

[0061] (5) Separate the solid and liquid of the above fermentation broth to obtain a clear fermentation broth with a pH of 3.6, a soluble solids content of 9.8%, and a total acid content of 1.23% (calculated as lactic acid).

[0062] Example 3

[0063] This embodiment provides a fermented composition for improving lung ventilation, wherein the total weight of yam, dried plum, Solomon's seal, ginkgo, and radish seed is 100g, specifically including 60 parts yam, 14 parts dried plum, 30 parts Solomon's seal, 10 parts ginkgo, 25 parts radish seed, 0.05 parts *Lactobacillus plantarum*, 0.15 parts *Bifidobacterium longum* subsp. *longum*, 0.1 parts *Lactococcus lactis* subsp. *lactolaccos*, 0.15 parts *Lactobacillus fermentum*, 1 part cellulase, 6 parts pectinase, 2 parts acidic protease, citric acid, and 2000 parts water. The preparation method is as follows:

[0064] (1) 60 parts of yam, 14 parts of dried plum, 30 parts of Solomon's seal, 10 parts of ginkgo and 25 parts of radish seed were crushed and passed through a 200-mesh sieve. They were then mixed with 2000 parts of water and 4 parts of sodium citrate were added to adjust the pH to 4.7 to obtain a mixed solution.

[0065] (2) Add 1 part cellulase, 6 parts pectinase and 2 parts acidic protease to the mixture for enzymatic hydrolysis. Hydrolyze at 60℃ for 150 min. After hydrolysis, add 4 parts sodium citrate to adjust the pH to 5.6 to obtain the hydrolysate. The cellulase activity is 18000 U / g, the pectinase activity is 23000 U / g, and the acidic protease activity is 12000 U / g.

[0066] (3) Heat the enzymatic hydrolysate to 95°C and stir for 30 min to obtain the extract;

[0067] (4) When the temperature drops to 35℃, add 0.05 parts of Lactobacillus plantarum, 0.15 parts of Bifidobacterium longum subsp. longum, 0.1 parts of Lactococcus lactis subsp. lactic acid, and 0.15 parts of Lactobacillus fermentum to the extract. Ferment at 35℃ for 72 hours to obtain the fermentation broth; wherein the viable count of Lactobacillus plantarum is 120 billion CFU / g, Bifidobacterium longum subsp. longum is 100 billion CFU / g, Lactococcus lactis subsp. lactic acid is 220 billion CFU / g, and Lactobacillus fermentum subsp. lactic acid is 100 billion CFU / g.

[0068] (5) Separate the solid and liquid of the above fermentation broth to obtain a clear fermentation broth with a pH of 3.1, a soluble solids content of 9.4%, and a total acid content of 1.13% (calculated as lactic acid).

[0069] Example 4

[0070] This embodiment provides a fermented composition for improving lung ventilation, wherein the total weight of yam, dried plum, Solomon's seal, ginkgo, and radish seed is 100g, specifically comprising 40 parts yam, 25 parts dried plum, 20 parts Solomon's seal, 25 parts ginkgo, 15 parts radish seed, 0.2 parts *Lactobacillus plantarum*, 0.6 parts *Bifidobacterium longum* subsp. *longum*, 0.4 parts *Lactococcus lactis* subsp. *lactolaccos*, 0.6 parts *Lactobacillus fermentum*, 3 parts cellulase, 4 parts pectinase, 1.5 parts acidic protease, 8.5 parts sodium citrate, and 2000 parts water. The preparation method is as follows:

[0071] (1) 40 parts of yam, 25 parts of dried plum, 20 parts of Solomon's seal, 25 parts of ginkgo and 15 parts of radish seed were crushed and passed through a 200-mesh sieve. They were then mixed with 2000 parts of water and 4.5 parts of sodium citrate were added to adjust the pH to 4.7 to obtain a mixed solution.

[0072] (2) Add 3 parts cellulase, 4 parts pectinase, and 1.5 parts acidic protease to the mixture for enzymatic hydrolysis. Hydrolyze at 65℃ for 250 min. After hydrolysis, add 4 parts sodium citrate to adjust the pH to 5.6 to obtain the hydrolysate. The cellulase activity is 18000 U / g, the pectinase activity is 23000 U / g, and the acidic protease activity is 12000 U / g.

[0073] (3) Heat the enzymatic hydrolysate to 110℃ and stir for 20 min to obtain the extract;

[0074] (4) After the temperature drops to 35℃, add 0.2 parts of Lactobacillus plantarum, 0.6 parts of Bifidobacterium longum subsp. longum, 0.4 parts of Lactococcus lactis subsp. lactic acid, and 0.6 parts of Lactobacillus fermentum to the extract. Ferment at 35℃ for 72 hours to obtain the fermentation broth; wherein the viable count of Lactobacillus plantarum is 120 billion CFU / g, Bifidobacterium longum subsp. longum is 100 billion CFU / g, Lactococcus lactis subsp. lactic acid is 220 billion CFU / g, and Lactobacillus fermentum subsp. lactic acid is 100 billion CFU / g.

[0075] (5) Separate the solid and liquid of the above fermentation broth to obtain a clear fermentation broth with a pH of 3.6, a soluble solids content of 9.7%, and a total acid content of 1.53% (calculated as lactic acid).

[0076] Example 5

[0077] This embodiment provides a product for improving lung ventilation function, wherein the total weight of yam, dried plum, Solomon's seal, ginkgo, and radish seed is 100g, specifically including 35 parts yam, 20 parts dried plum, 15 parts Solomon's seal, 15 parts ginkgo, 10 parts radish seed, 0.1 parts Lactobacillus plantarum, 0.3 parts Bifidobacterium longum subsp. longum, 0.2 parts Lactococcus lactis subsp. lactis, 0.3 parts Lactobacillus fermentum, 2.2 parts cellulase, 2.2 parts pectinase, 1 part acidic protease, 8.5 parts sodium citrate, 10 parts apple powder, 15 parts pear powder, 15 parts loquat powder, 20 parts erythritol, 45 parts fructooligosaccharides, and 2000 parts water. The preparation method is as follows:

[0078] (1) 35 parts of yam, 20 parts of dried plum, 15 parts of Solomon's seal, 15 parts of ginkgo and 10 parts of radish seed were crushed and passed through a 200-mesh sieve. They were then mixed with 2000 parts of water and 4.5 parts of sodium citrate were added to adjust the pH to 4.7 to obtain a mixed solution.

[0079] (2) Add 2.2 parts of cellulase, 2.2 parts of pectinase, and 1 part of acidic protease to the mixture for enzymatic hydrolysis. Hydrolyze at 58℃ for 150 min. After hydrolysis, add 4 parts of sodium citrate to adjust the pH to 5.6 to obtain the hydrolysate. The cellulase activity is 18000 U / g, the pectinase activity is 23000 U / g, and the acidic protease activity is 12000 U / g.

[0080] (3) Heat the enzymatic hydrolysate to 95°C and stir for 30 min to obtain the extract;

[0081] (4) After the temperature drops to 35℃, add 0.1 parts of Lactobacillus plantarum, 0.3 parts of Bifidobacterium longum subsp. longum, 0.2 parts of Lactococcus lactis subsp. lactic acid, and 0.3 parts of Lactobacillus fermentum to the extract. Ferment at 35℃ for 72 hours to obtain the fermentation broth; wherein the viable count of Lactobacillus plantarum is 120 billion CFU / g, Bifidobacterium longum subsp. longum is 100 billion CFU / g, Lactococcus lactis subsp. lactic acid is 220 billion CFU / g, and Lactobacillus fermentum subsp. lactic acid is 100 billion CFU / g.

[0082] (5) Separate the solid and liquid components of the above fermentation broth to obtain a clear fermentation broth. Add 10 parts apple powder, 15 parts pear powder, 15 parts loquat powder, 20 parts erythritol, and 45 parts fructooligosaccharides to the clear fermentation broth and stir for 30 minutes until well mixed. The density is 1.05 g / cm³. 3 The soluble solids content is 22%.

[0083] Comparative Example 1

[0084] This comparative example provides a fermented composition for improving lung ventilation. The difference between this composition and Example 1 lies in the raw materials. This comparative example includes 35 parts of yam, 20 parts of dried plum, and 15 parts of Solomon's seal rhizome, but the total weight of these three raw materials is the same as the total weight of the five raw materials (yam, dried plum, Solomon's seal rhizome, ginkgo, and radish seed) in Example 1. All other aspects are the same as in Example 1.

[0085] Comparative Example 2

[0086] This comparative example provides a fermented composition for improving lung ventilation. The difference between this composition and Example 1 lies in the raw materials. This comparative example includes 35 parts of yam, 20 parts of dried plum, 15 parts of Solomon's seal rhizome, and 10 parts of ginkgo seed. However, the total weight of these four raw materials is the same as the total weight of the five raw materials (yam, dried plum, Solomon's seal rhizome, ginkgo seed, and radish seed) in Example 1. All other aspects are the same as in Example 1.

[0087] Comparative Example 3

[0088] This comparative example provides a fermented composition for improving lung ventilation. The difference between this composition and Example 1 lies in the raw materials. This comparative example includes 35 parts of yam, 20 parts of dried plum, 15 parts of Solomon's seal rhizome, and 10 parts of radish seed. However, the total weight of the four raw materials is the same as the total weight of the five raw materials (yam, dried plum, Solomon's seal rhizome, ginkgo, and radish seed) in Example 1. All other aspects are the same as in Example 1.

[0089] Comparative Example 4

[0090] This comparative example provides a fermented composition for improving lung ventilation. The difference between this composition and Example 1 lies in the composition of the fermentation starter culture; in this comparative example, the fermentation starter culture is 0.9 parts of *Lactobacillus plantarum*. All other aspects are the same as in Example 1.

[0091] Comparative Example 5

[0092] This comparative example provides a fermentation composition for improving pulmonary ventilation. The difference between this composition and Example 1 lies in the composition of the fermentation starter culture. In this comparative example, the fermentation starter culture consists of 0.9 parts of *Bifidobacterium longum* subsp. *longum*. All other components are the same as in Example 1.

[0093] Comparative Example 6

[0094] This comparative example provides a fermentation composition for improving lung ventilation. The difference between this composition and Example 1 lies in the composition of the fermentation starter culture. In this comparative example, the fermentation starter culture consists of 0.9 parts of *Lactococcus lactis* subsp. *lactospirae*. All other components are the same as in Example 1.

[0095] Comparative Example 7

[0096] This comparative example provides a fermented composition for improving lung ventilation. The difference between this composition and Example 1 lies in the composition of the fermentation starter culture. In this comparative example, the fermentation starter culture consists of 0.9 parts of *Lactobacillus fermentatus*. All other aspects are the same as in Example 1.

[0097] Comparative Example 8

[0098] This comparative example provides a fermentation composition for improving lung ventilation. The difference between this composition and Example 1 lies in the composition of the fermentation starter culture. In this comparative example, the fermentation starter culture consists of 0.2 parts of *Lactobacillus plantarum*, 0.3 parts of *Bifidobacterium longum* subsp. *longum*, 0.2 parts of *Lactococcus lactis* subsp. *lactolaccos*, and 0.2 parts of *Lactobacillus fermentum*. All other components are the same as in Example 1.

[0099] Comparative Example 9

[0100] This comparative example provides a fermentation composition for improving lung ventilation. The difference between this composition and Example 1 lies in the enzyme preparation composition. In this comparative example, the enzyme preparation includes 2.2 parts cellulase and 2.2 parts pectinase, but the total weight is the same as in Example 1. All other aspects are the same as in Example 1.

[0101] Experimental Example 1

[0102] Animal experiments:

[0103] Experimental design for validating lung function using a rat model:

[0104] (1) Grouping, modeling and intervention methods: 210 rats were randomly divided into 15 groups of 14 rats each using a random number table method, namely, normal group, asthma group and experimental group. The experimental group included fermentation composition example 1 group, fermentation composition example 2 group, fermentation composition example 3 group, fermentation composition example 4 group, fermentation composition comparative example 1 group, fermentation composition comparative example 2 group, fermentation composition comparative example 3 group, fermentation composition comparative example 4 group, fermentation composition comparative example 5 group, fermentation composition comparative example 6 group, fermentation composition comparative example 7 group, fermentation composition comparative example 8 group, and fermentation composition comparative example 9 group. After acclimatization for 1 week, the rats in the asthma group and experimental group were sensitized by intraperitoneal injection of 1 mL sensitizing solution (containing 100 mg ovalbumin (OVA) and 100 mg aluminum hydroxide) on days 1 and 8 of the experiment. From day 15, they were challenged by nebulization with 1% OVA for 30 min / time, once every other day, for a total of 7 weeks.

[0105] The experimental group rats were administered the corresponding fermented composition by gavage once daily starting from day 15 of the experiment, for 7 weeks. The gavage dosage for rats was calculated based on the human-to-rat body surface area conversion factor of 0.018 and the usual adult dosage, with a gavage volume of 63 mL / kg. The asthma group received the same volume of physiological saline instead of the gavage solution; the normal control group received the same volume of physiological saline instead of the sensitizing solution, provocation solution, and gavage solution.

[0106] Four rats were randomly selected from each group and were not treated with the relevant drugs. Lung function was measured on day 15 and day 29 after nebulization to determine the success of the model. The success rate of modeling in each group was 100%.

[0107] (2) Lung function testing and tissue sampling

[0108] Ten rats were randomly selected from each experimental group for pulmonary ventilation function testing and tissue sampling. Twenty-four hours after the last gavage, the rats were anesthetized by intraperitoneal injection of 1% sodium pentobarbital solution (60 mg / kg). About 5 minutes later, endotracheal intubation was performed, and the rats were placed supine in a body plethysmography chamber. The endotracheal tube was connected to the airway of the body plethysmography chamber, and data collection began after the data curve stabilized. Forced expiratory volume in 0.3 seconds (FEV0.3), forced vital capacity (FEV), maximum expiratory flow (PEF), and minute ventilation (MV) were recorded.

[0109] (3) Data statistics and analysis

[0110] SPSS 19.0 software was used for statistical analysis. Quantitative data were analyzed using... ± s indicates that if the data in each group conform to a normal distribution and homogeneity of variance, a one-way ANOVA is performed, and the LSD test is used for comparisons between groups. If the variances are not homogeneous, a non-parametric test (Kruskal-Wallis one-way ANOVA) is used. P < 0.05 is considered statistically significant.

[0111] (4) Results and Discussion

[0112] The lung function results of each group of mice are shown in Table 1.

[0113] Table 1. Comparison of lung function levels (FEV0.3, FVC, PEF, MV) among different groups of rats ±s)

[0114]

[0115] Note: Compared with the asthma group, * P < 0.05; compared with Example 1, ^ P < 0.05.

[0116] Data Results Analysis: Compared with the asthma group, the FEV1, FVC, PEF, and MV of Examples 1-4 showed significant differences (P < 0.05), indicating that the fermented composition obtained by this invention can improve lung ventilation function, thereby effectively relieving symptoms such as difficulty breathing and shortness of breath, and playing a role in nourishing and moisturizing the lungs. Comparative Examples 1-9 showed significant differences compared with Example 1, meaning that the raw materials in the fermented composition provided by this invention work synergistically to improve lung ventilation function. Examples 2-4 showed significant differences compared with Example 1, indicating that the raw material ratio in Example 1 was the optimal choice. In summary, the fermented product obtained by this invention has a good effect on nourishing and moisturizing the lungs and can effectively relieve symptoms of poor lung function such as difficulty breathing and shortness of breath.

[0117] This invention is not limited to the above-described embodiments. Any modifications, improvements, or substitutions that can be conceived by those skilled in the art without departing from the essential content of this invention fall within the scope of this invention.

Claims

1. A fermentation composition for improving lung ventilation, characterized in that, The product, by weight, is composed of 20-60 parts of yam, 15-40 parts of dried plum, 10-30 parts of Solomon's seal rhizome, 10-30 parts of ginkgo, 5-25 parts of radish seed, 0.3-3 parts of fermentation starter culture, and 3.1-13 parts of enzyme preparation. The fermentation starter culture consists of 0.05-0.5 parts of *Lactobacillus plantarum*, 0.1-0.9 parts of *Bifidobacterium longum* subsp. *longum*, 0.1-0.6 parts of *Lactococcus lactis* subsp. *lactolaccos*, and 0.1-0.9 parts of *Lactobacillus fermentum*. The enzyme preparation consists of 1-5 parts of cellulase, 2-6 parts of pectinase, and 0.1-2 parts of acidic protease.

2. The composition according to claim 1, characterized in that, The weight ratio of *Lactobacillus plantarum*, *Bifidobacterium longum* subsp. *longum*, *Lactococcus lactis* subsp. *lactosporum*, and *Lactobacillus fermentum* is 1:3:2:3; the bacterial activity of *Lactobacillus plantarum* is 100-300 billion CFU / g, the bacterial activity of *Bifidobacterium longum* subsp. *longum* is 100-400 billion CFU / g, the bacterial activity of *Lactococcus lactis* subsp. *lactosporum* is 80-300 billion CFU / g, and the bacterial activity of *Lactobacillus fermentum* is 50-200 billion CFU / g.

3. The composition according to claim 1, characterized in that, The weight ratio of cellulase, pectinase, and acidic protease is 2.2:2.2:1; the enzyme activity of cellulase is 10000-28000 U / g, the enzyme activity of pectinase is 5000-40000 U / g, and the enzyme activity of acidic protease is 5000-20000 U / g.

4. A method for preparing the fermentation composition according to any one of claims 1-3, characterized in that, Includes the following steps: (1) Crush yam, dried plum, Solomon's seal, ginkgo and radish seeds, sieve to 100-200 mesh, add water and mix evenly, then add acid-base adjuster to pH 4.0-5.0 to obtain a mixed solution; (2) Add an enzyme preparation to the mixture for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, add an acid-base adjuster to adjust the pH to 5.0-6.0 to obtain the enzymatic hydrolysate. (3) Extract the enzyme hydrolysate to obtain an extract; (4) Add fermentation bacteria powder to the extract to ferment and obtain fermentation liquid, and separate the solid and liquid of the fermentation liquid to obtain fermentation clear liquid.

5. The preparation method according to claim 4, characterized in that, The enzymatic hydrolysis conditions in step (2) are 35-65℃ and 100-260 min; the extraction conditions in step (3) are 75-110℃ and 15-90 min; the fermentation temperature in step (4) is 25-42℃ and the time is 48-96 h; the acid-base regulator is sodium citrate.

6. A product for improving lung ventilation function, characterized in that, The fermentation composition for improving lung ventilation as described in any one of claims 1-3.

7. The product for improving lung ventilation function according to claim 6, characterized in that, It also includes flavoring agents and / or sweeteners; the flavoring agents are selected from one or more of apple powder, pear powder, orange powder, loquat powder or pomegranate powder; the sweeteners are selected from one or more of erythritol, xylitol, sorbitol, xylooligosaccharides, fructooligosaccharides or inulin.

8. The product for improving lung ventilation function according to claim 7, characterized in that, The flavoring agent comprises 8-10 parts apple powder, 10-15 parts pear powder and 12-18 parts loquat powder; the sweetener comprises 18-25 parts erythritol and 40-50 parts fructooligosaccharides.

9. A method for preparing the product for improving pulmonary ventilation function as described in any one of claims 6-8, characterized in that, Includes the following steps: (1) Crush yam, dried plum, Solomon's seal, ginkgo and radish seeds, sieve to 100-200 mesh, add water and mix evenly, then add acid-base adjuster to pH 4.0-5.0 to obtain a mixed solution; (2) Add an enzyme preparation to the mixture for enzymatic hydrolysis. After the enzymatic hydrolysis is completed, add an acid-base adjuster to adjust the pH to 5.0-6.0 to obtain the enzymatic hydrolysate. (3) Extract the enzyme hydrolysate to obtain an extract; (4) Add fermentation liquid powder to the extract to ferment and obtain fermentation liquid, and separate the solid and liquid of the fermentation liquid to obtain fermentation clear liquid; (5) Add flavoring agent and / or sweetener to the fermentation liquid and mix well to obtain the final product; In step (2), the enzymatic hydrolysis conditions are 35-65℃ for 100-260 min; in step (3), the extraction temperature is 75-110℃ for 15-90 min; in step (4), the fermentation temperature is 25-42℃ for 48-96 h; and the acid-base regulator is sodium citrate.

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