A kit for detecting plant pathogenic bacteria and a method for detecting the same

Abstract

The application relates to the technical field of plant pathogenic bacteria detection, in particular to a plant pathogenic bacteria detection kit convenient to detect and a detection method thereof. The kit comprises a lower base and an upper base, the upper base and the lower base are movably installed together through buckles, a clearance groove is formed in the upper end of the lower base, a recess is formed in one side of the clearance groove, one end of a guide spring is fixedly connected with a cover plate, the other end is fixedly connected with a cavity, when a reagent bottle completely enters the cavity, first and second baffle plates are movably connected in the cavity under the elastic force of the guide spring; a rotating disc is driven to rotate through a motor, so that a plurality of groups of reagent bottles are driven to move, when a reagent bottle to be used moves to the position of a glass window, the motor is turned off, at the moment, a guide wheel located at the position of the glass window enters the recess, external substances such as dust and insects are prevented from entering the cavity, so that the reagent is prevented from being damaged or deteriorated.

Description

Technical Field

[0001] This invention relates to the field of plant pathogenic bacteria detection technology, specifically to a reagent kit and detection method for easy detection of plant pathogenic bacteria. Background Technology

[0002] For example, Chinese patent CN212051350U discloses a plant pathogenic bacteria detection kit, which includes a detection kit body, a top cover installed on the top of the detection kit body, a dilution plate installed at the bottom inside the detection kit body, and a bottom plate installed above the dilution plate. The bottom plate has a screw hole at the top, and a round rod is threadedly connected to the screw hole. A screw is installed at the bottom end of the round rod. A second partition is installed above the bottom plate, and a second reagent bottle is placed on the top of the second partition.

[0003] However, the above solution has the following shortcomings: In the above patent, reagent bottles are placed sequentially on the first and second partitions. When a reagent is needed, the round rod is pulled upwards to lift all items above the bottom plate out of the test kit body. However, this method is not convenient for operators to quickly find the required reagent. Operators have to search for the required reagent one by one among all the pulled-out reagents, which is not convenient to use. At the same time, when testing is required in an external environment, this method will expose all reagents to the external environment. When the reagent is pushed back into the test kit, dust, insects and other external substances will be brought into the test kit, which may cause the reagent to be damaged or deteriorated and become unusable. Therefore, we have introduced a test kit for detecting plant pathogenic bacteria that is easy to detect, as well as its detection method and leveling machine. Summary of the Invention

[0004] The purpose of this invention is to provide a reagent kit and detection method for detecting plant pathogenic bacteria that are easy to use, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A reagent kit for detecting plant pathogenic bacteria, which is easy to detect, includes a lower base and an upper base, which are movably installed together by a snap fastener. The lower base has a relief groove in its upper end and a recess in one side of the relief groove. A turntable is movably connected in the relief groove. The upper base has a connecting cavity in its lower end. The turntable has several sets of placement holes in its upper end for placing reagent bottles. An arc-shaped clamp is provided in each placement hole to hold the reagent bottle. The side of the arc-shaped clamp near the placement hole is fixedly connected to two sets of T-shaped rods, and the end of the T-shaped rod away from the arc-shaped clamp is movably connected to a sliding rod.

[0007] The slide bar is movably connected to a guide wheel at the end away from the T-shaped bar. The guide wheel contacts the clearance groove. A cross-shaped guide groove is provided in the placement hole. A pushing mechanism is provided on the lower side of the turntable. The reagent bottle is pushed upward by the pushing mechanism. A cavity is provided in the upper end of the upper base. A guide cavity is provided in the lower end of the cavity. The lower end of the guide cavity is connected to the connecting cavity. A cover plate is provided on the upper side of the guide cavity.

[0008] The cover plate is movably connected to the cavity. When the reagent bottle moves upward, it pushes the cover plate open. An arc-shaped rod is fixedly connected to the upper end of the cover plate. The arc-shaped rod slides in an arc-shaped groove. The arc-shaped groove is opened in the upper base. A guide spring is sleeved on the outside of the arc-shaped rod. One end of the guide spring is fixedly connected to the cover plate, and the other end is fixedly connected to the cavity. When the reagent bottle is completely inserted into the cavity, under the elastic force of the guide spring, the cover plate pushes the reagent bottle to the left. A first baffle and a second baffle are movably connected to the cavity.

[0009] Preferably, the lower end of the turntable is fixedly connected to the output end of the motor, and the motor is fixedly installed inside the lower base.

[0010] Preferably, the T-shaped rod is slidably connected to the sliding cavity, the sliding cavity is opened inside the slide rod, and a first spring is fixedly connected inside the sliding cavity, with the other end of the first spring fixedly connected to the T-shaped rod.

[0011] Preferably, a limiting ring is sleeved on the outer side of the slide rod, the limiting ring slides within a limiting cavity, the limiting cavity is located within the turntable, and a second spring is sleeved on the outer side of the slide rod, one end of the second spring being fixedly connected to the limiting ring and the other end being fixedly connected to the limiting cavity.

[0012] Preferably, the pushing mechanism includes a cross-shaped push plate, the lower end of which is fixedly connected to an L-shaped push rod. The L-shaped push rod is slidably connected in a connecting groove, which is opened in the lower base. A return spring is fixedly connected in the connecting groove, and the other end of the return spring is fixedly connected to the connecting groove.

[0013] Preferably, the upper base has a storage cavity at its upper end, and a sealing cover is snapped onto the upper end of the storage cavity. A glass window is fixedly installed on one side of the upper base, and the glass window corresponds to the position of the connecting cavity.

[0014] Furthermore, to achieve the above objectives, the present invention also provides a detection method for detecting plant pathogenic bacteria based on the easily detectable plant pathogenic bacteria detection kit described in any one of the above claims, comprising:

[0015] S1. Open the buckle to separate the upper base from the lower base, place several sets of reagent bottles into several sets of placement holes, and use the arc-shaped clamp to hold the reagent bottles in the placement holes. After placement, reassemble the upper base and the lower base together using the buckle.

[0016] S2. When the reagent in the reagent bottle needs to be used, turn on the motor to start the turntable to rotate. The rotation of the turntable will drive several sets of reagent bottles to rotate. Rotate the reagent bottle to be used through the glass window to the position of the glass window. After the guide wheel rotates to the position of the groove, the arc-shaped clamp will release the pressure on the reagent bottle. After the rotation is completed, turn off the motor.

[0017] S3. Push the L-shaped push rod upward to move the cross-shaped push plate upward. As the cross-shaped push plate moves upward, the reagent bottle in the placement hole will be pushed upward. After the reagent bottle enters the guide cavity, as the reagent bottle moves upward, the cover will be pushed open. Pull the first baffle upward. When the reagent bottle is completely pushed into the cavity, under the elastic force of the guide spring, the cover will push the reagent bottle to the left, open the second baffle and take out the reagent bottle. After the reagent bottle is taken out, put the pathogen sample collected from the plant sample into the reagent bottle. By observing the color change of the sample in the reagent, it can be determined whether the target pathogen is present in the sample.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention uses an electric motor to drive a turntable to rotate, causing several sets of reagent bottles to move. When the reagent bottle to be used moves to the position of the glass window, the electric motor is turned off. At this time, the guide wheel located at the glass window position enters the groove, causing the arc-shaped clamp to release its grip on the reagent bottle. By pushing the L-shaped push rod upward, the reagent bottle can be moved upward. During the movement, the reagent bottle pushes open the cover plate, causing the guide spring to be compressed. When the reagent bottle is completely inside the cavity, the first baffle is pulled upward, causing the cover plate to push the reagent bottle into the left side of the cavity under the elastic force of the guide spring. At the same time, the cover plate is put back on the upper end of the guide cavity. By pulling the second baffle upward, the reagent bottle can be taken out for use. This prevents dust, insects and other external substances from entering the cavity, which could cause damage or deterioration of the reagent. Attached Figure Description

[0019] Figure 1 This is a top-section structural diagram of the present invention;

[0020] Figure 2 This is a top-section structural diagram of the present invention;

[0021] Figure 3 For the present invention Figure 2 Enlarged structural diagram at point A in the middle;

[0022] Figure 4This is a cross-sectional structural diagram showing the connection between the slide bar and the turntable in this invention;

[0023] Figure 5 This is a schematic diagram of the connection between the cover plate and the arc-shaped rod of the present invention;

[0024] Figure 6 This is a cross-sectional view of the reagent bottle in its placement state according to the present invention;

[0025] Figure 7 This is a cross-sectional view of the reagent bottle of the present invention being pushed into the cavity.

[0026] In the diagram: 1. Lower base; 2. L-shaped push rod; 3. Connecting groove; 4. Return spring; 5. Motor; 6. Cross-shaped push plate; 7. Placement hole; 8. Glass window; 9. Guide cavity; 10. Second baffle; 11. First baffle; 12. Cavity; 13. Cover plate; 14. Guide spring; 15. Arc-shaped groove; 16. Arc-shaped clamp; 17. Turntable; 18. Cross-shaped guide groove; 19. Connecting cavity; 20. Upper base; 21. Storage cavity; 22. Sealing cover plate; 23. Buckle; 24. T-shaped rod; 25. Sliding cavity; 26. First spring; 27. Guide wheel; 28. Slide rod; 29. ​​Limiting cavity; 30. Second spring; 31. Limiting ring; 32. Arc-shaped rod; 33. Groove; 34. Relief groove; 35. Reagent bottle. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Please see Figure 1-7 The present invention provides a technical solution:

[0029] Example 1:

[0030] A reagent kit for detecting plant pathogenic bacteria, which is easy to use, includes a lower base 1 and an upper base 20. The upper base 1 and the lower base 20 are movably installed together by a snap fastener 23. A clearance groove 34 is formed in the upper end of the lower base 1, and a recess 33 is formed in one side of the clearance groove 34. When the guide wheel 27 rotates to the position of the recess 33, the arc-shaped clamp 16 will release the pressure on the reagent bottle 35. A turntable 17 is movably connected in the clearance groove 34. A connecting cavity 19 is formed in the lower end of the upper base 20. Several sets of placement holes 7 are opened in the upper end of the tray 17. The placement holes 7 are used to place reagent bottles 35. An arc-shaped clamp 16 is provided in the placement hole 7. The reagent bottle 35 can be clamped by the arc-shaped clamp 16. The side of the arc-shaped clamp 16 near the placement hole 7 is fixedly connected to two sets of T-shaped rods 24. The end of the T-shaped rod 24 away from the arc-shaped clamp 16 is movably connected to the slide rod 28. When the diameter of the reagent bottle 35 is large, after the arc-shaped clamp 16 contacts the reagent bottle 35, the slide rod 28 moves continuously.

[0031] The T-shaped rod 24 will move into the limiting cavity 29. At this time, the first spring 26 is compressed. The end of the slide rod 28 away from the T-shaped rod 24 is movably connected to the guide wheel 27. The guide wheel 27 contacts the relief groove 34. A cross-shaped guide groove 18 is opened in the placement hole 7. A pushing mechanism is provided on the lower side of the turntable 17. The reagent bottle 35 is pushed upward by the pushing mechanism. A cavity 12 is opened in the upper end of the upper base 20. A guide cavity 9 is opened in the lower end of the cavity 12. The lower end of the guide cavity 9 is connected to the connecting cavity 19. A cover plate 13 is provided on the upper side of the guide cavity 9.

[0032] The cover plate 13 is movably connected to the cavity 12. When the reagent bottle 35 moves upward, it pushes the cover plate 13 open. An arc-shaped rod 32 is fixedly connected to the upper end of the cover plate 13. The arc-shaped rod 32 slides in the arc-shaped groove 15, which is opened on the upper base 20. A guide spring 14 is sleeved on the outside of the arc-shaped rod 32. One end of the guide spring 14 is fixedly connected to the cover plate 13, and the other end is fixedly connected to the cavity 12. When the reagent bottle 35 is completely inserted into the cavity 12, under the elastic force of the guide spring 14, the cover plate 13 pushes the reagent bottle 35 to the left. The cavity 12 is movably connected to the first baffle 11 and the second baffle 10 respectively. When the L-shaped push rod 2 is pushed upward, the first baffle 11 is pulled upward, so that the reagent bottle 35 can be pushed into the left side of the cavity 12 by the cover plate 13. After it is pushed in, the second baffle 10 is pulled upward to remove the reagent bottle 35, thus preventing dust or insects from the external environment from entering the connecting cavity 19.

[0033] Example 2:

[0034] Based on Example 1, in order to enable the arc-shaped clamping plate 16 to clamp reagent bottles 35 of different diameters, the lower end of the turntable 17 is fixedly connected to the output end of the motor 5. The motor 5 is fixedly installed in the lower base 1. The model of the motor 5 can be conventionally selected according to the size of the equipment and the actual power. The rotation of the motor 5 drives the turntable 17 to start rotating. During the rotation of the turntable 17, several groups of reagent bottles 35 can be driven to rotate synchronously. The T-shaped rod 24 is slidably connected in the sliding cavity 25. The sliding cavity 25 is opened in the slide rod 28. The first spring 26 is fixedly connected in the sliding cavity 25. The other end of the first spring 26 is fixedly connected to the T-shaped rod 24. When the diameter of the reagent bottle 35 is large, after the arc-shaped clamping plate 16 contacts the reagent bottle 35, the T-shaped rod 24 will move into the limiting cavity 29 as the slide rod 28 moves continuously. At this time, the first spring 26 is compressed.

[0035] A limiting ring 31 is sleeved on the outside of the slide rod 28. The limiting ring 31 slides in the limiting cavity 29, which is located in the turntable 17. A second spring 30 is sleeved on the outside of the slide rod 28. One end of the second spring 30 is fixedly connected to the limiting ring 31, and the other end is fixedly connected to the limiting cavity 29. When the guide wheel 27 moves to the position of the groove 33, the slide rod 28 will move outward under the elastic force of the second spring 30, and drive the guide wheel 27 to move synchronously, so that the arc-shaped clamp 16 releases its grip on the reagent bottle 35.

[0036] The pushing mechanism includes a cross-shaped push plate 6, the lower end of which is fixedly connected to an L-shaped push rod 2. The L-shaped push rod 2 is slidably connected in a connecting groove 3, which is located in the lower base 1. A return spring 4 is fixedly connected in the connecting groove 3, and the other end of the return spring 4 is fixedly connected to the connecting groove 3. A storage cavity 21 is provided in the upper end of the upper base 20, and a sealing cover 22 is snapped onto the upper end of the storage cavity 21. The test item can be placed into the storage cavity 21 by opening the sealing cover 22. A glass window 8 is fixedly installed in one side of the upper base 20, and the glass window 8 corresponds to the position of the connecting cavity 19.

[0037] Furthermore, to achieve the above objectives, the present invention also provides a detection method for detecting plant pathogenic bacteria based on the aforementioned easy-to-detect plant pathogenic bacteria detection kit, comprising:

[0038] S1. Open the buckle 23 to separate the upper base 20 from the lower base 1, and put several sets of reagent bottles 35 into several sets of placement holes 7. The arc-shaped clamp 16 clamps the reagent bottles 35 in the placement holes 7. After placement, the upper base 20 and the lower base 1 are reinstalled together by using the buckle 23.

[0039] S2. When the reagent in the reagent bottle 35 needs to be used, turn on the motor 5 to drive the turntable 17 to start rotating. The rotation of the turntable 17 drives several sets of reagent bottles 35 to rotate. The reagent bottle 35 to be used is rotated to the position of the glass window 8 through the glass window 8. After the guide wheel 27 rotates to the position of the groove 33, the arc-shaped clamp 16 will release the pressure on the reagent bottle 35. After the rotation is completed, turn off the motor 5.

[0040] S3. Pushing the L-shaped push rod 2 upwards causes the cross-shaped push plate 6 to move upwards. The cross-shaped push plate 6 moves upwards and passes through the cross-shaped guide groove 18 into the placement hole 7. As the cross-shaped push plate 6 moves upwards, the reagent bottle 35 located in the placement hole 7 is pushed upwards. When the reagent bottle 35 enters the guide cavity 9, as the reagent bottle 35 moves upwards, the cover plate 13 is pushed open. Pulling the first baffle 11 upwards, when the reagent bottle 35 is completely pushed into the cavity 12, under the elastic force of the guide spring 14, the cover plate 13 pushes the reagent bottle 35 to the left, releasing the push and pull on the L-shaped push rod 2 to return it to its original position. Opening the second baffle 10 allows the reagent bottle 35 to be taken out. After the reagent bottle 35 is taken out, the pathogen sample collected from the plant sample is placed into the reagent bottle 35. By observing the color change of the sample in the reagent bottle 35, it is determined whether the target pathogen exists in the sample.

[0041] Working principle: When in use, the upper base 20 and the lower base 1 can be separated by opening the buckle 23. After the upper base 20 and the lower base 1 are separated, a set of reagent bottles 35 are placed in the placement hole 7 near the glass window 8. After placement, the motor 5 is turned on to drive the turntable 17 to start rotating, so that the other set of placement holes 7 rotates to the position of the glass window 8. The other set of reagent bottles 35 are placed into the placement hole 7 and the turntable 17 is rotated again. During the rotation of the turntable 17, the guide wheel 27 located in the groove 33 will rotate into the clearance groove 34. At this time, the slide bar 28 is squeezed and moves inward. The movement of the slide bar 28 drives the T-shaped bar 24 to move inward. At this time, the arc-shaped clamp 16 clamps the reagent bottle 35 in the placement hole 7.

[0042] After all the reagent bottles 35 are placed into the placement hole 7, the upper base 20 and the lower base 1 can be installed together by the buckle 23. When the reagent in the reagent bottle 35 needs to be used, the motor 5 is turned on to drive the turntable 17 to start rotating. During the rotation of the turntable 17, several sets of reagent bottles 35 will start to rotate. The reagent bottle 35 to be used can be rotated to the position of the glass window 8. After the guide wheel 27 rotates to the position of the groove 33, the arc-shaped clamp 16 will release the pressure on the reagent bottle 35. After the rotation is completed, the motor 5 can be turned off. Push the L-shaped push rod 2 upward to drive the cross-shaped push plate 6 to move upward. The cross-shaped push plate 6 moves upward and passes through the cross-shaped guide groove 18 into the placement hole 7. At this time, as the cross-shaped push plate 6 continues to move upward, the reagent bottle 35 in the placement hole 7 will be pushed upward. When the reagent bottle 35 enters the guide cavity 9, as the reagent bottle 35 continues to move upward, the cover plate 13 will be pushed open.

[0043] At this point, pull the first baffle 11 upwards. When the reagent bottle 35 is fully pushed into the cavity 12, the cover 13 will move downwards under the elastic force of the guide spring 14 and push the reagent bottle 35 to the left. Release the push and pull on the L-shaped push rod 2. At this point, under the elastic force of the reset spring 4, the L-shaped push rod 2 will return to its original position. Then, open the second baffle 10 and take out the reagent bottle 35.

[0044] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A reagent kit for detecting plant pathogenic bacteria, comprising a lower base and an upper base, wherein the upper base and the lower base are movably connected together by a snap fastener, characterized in that: The lower base has a clearance groove in its upper end and a recess in one side of the clearance groove. A turntable is movably connected in the clearance groove. The upper base has a connecting cavity in its lower end. The turntable has several sets of placement holes in its upper end for placing reagent bottles. An arc-shaped clamp is provided in each placement hole to hold the reagent bottle. The side of the arc-shaped clamp near the placement hole is fixedly connected to two sets of T-shaped rods. The end of the T-shaped rod away from the arc-shaped clamp is movably connected to a sliding rod. The slide bar is movably connected to a guide wheel at the end away from the T-shaped bar. The guide wheel contacts the clearance groove. A cross-shaped guide groove is provided in the placement hole. A pushing mechanism is provided on the lower side of the turntable. The reagent bottle is pushed upward by the pushing mechanism. A cavity is provided in the upper end of the upper base. A guide cavity is provided in the lower end of the cavity. The lower end of the guide cavity is connected to the connecting cavity. A cover plate is provided on the upper side of the guide cavity. The pushing mechanism includes a cross-shaped push plate, the lower end of which is fixedly connected to an L-shaped push rod. The L-shaped push rod slides in a connecting groove, which is located in the lower base. A return spring is fixedly connected in the connecting groove, and the other end of the return spring is fixedly connected to the connecting groove. A storage cavity is provided in the upper end of the upper base, and a sealing cover is snapped onto the upper end of the storage cavity. A glass window is fixedly installed in one side of the upper base, and the glass window corresponds to the position of the connecting cavity. The cover plate is movably connected to the cavity. When the reagent bottle moves upward, it pushes the cover plate open. An arc-shaped rod is fixedly connected to the upper end of the cover plate. The arc-shaped rod slides in an arc-shaped groove. The arc-shaped groove is opened in the upper base. A guide spring is sleeved on the outside of the arc-shaped rod. One end of the guide spring is fixedly connected to the cover plate, and the other end is fixedly connected to the cavity. When the reagent bottle moves to the position of the glass window, the guide wheel located at the glass window position enters the groove, so that the arc-shaped clamp releases the clamp on the reagent bottle. Pushing the L-shaped push rod upward can drive the reagent bottle to move upward. The reagent bottle enters the guide cavity and pushes the cover plate open. When the reagent bottle is completely inside the cavity, under the elastic force of the guide spring, the cover plate pushes the reagent bottle to the left. A first baffle and a second baffle are movably connected inside the cavity.

2. The reagent kit for detecting plant pathogenic bacteria according to claim 1, characterized in that: The lower end of the turntable is fixedly connected to the output end of the motor, and the motor is fixedly installed inside the lower base.

3. The reagent kit for detecting plant pathogenic bacteria according to claim 1, characterized in that: The T-shaped rod is slidably connected to the sliding cavity, which is opened inside the sliding rod. A first spring is fixedly connected inside the sliding cavity, and the other end of the first spring is fixedly connected to the T-shaped rod.

4. The reagent kit for detecting plant pathogenic bacteria according to claim 3, characterized in that: A limiting ring is sleeved on the outside of the slide rod, and the limiting ring slides in the limiting cavity, which is located inside the turntable. A second spring is sleeved on the outside of the slide rod, with one end of the second spring fixedly connected to the limiting ring and the other end fixedly connected to the limiting cavity.

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