Fixing and inhaling device for fruit fly's glossary reflex experiment and experimental method thereof

Abstract

The application provides a fruit fly ecdysial reflex experiment fixing and air suction device and an experiment method thereof, and belongs to the technical field of animal experiment devices. The existing fruit fly ecdysial reflex experiment operation process is complicated, errors are prone to occur, fruit flies escape or are fixed for multiple times, and the fruit fly activity, fruit fly light-avoiding quiet time and other problems are caused. The device comprises a fixing device and an air suction device. The fixing device is made of a sponge material without peculiar smell, and the air suction device is made of a transparent polypropylene material. The fixing device is conical in shape, the air suction device is cylindrical in shape, the suction head of the air suction device is concave downward to form a hemisphere, and the head of the fruit fly can be just wrapped. The application is simple and convenient to operate, reduces uncertain influencing factors, greatly improves the experiment efficiency, and is easy to make due to rich raw material sources.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal experiment device, in particular to a fruit fly proboscis extension reflex experiment fixing and air suction device. BACKGROUND

[0002] The fruit fly has sensitive taste and smell, and the existing behavior paradigm can be used to track and manipulate the behavior neural circuit, so that the fruit fly becomes an attractive model for studying taste and smell. The proboscis extension reflex experiment mainly uses the fruit fly to smell and taste a certain substance to perform feeding behavior, and the number of proboscis extensions of the fruit fly is recorded to determine the degree of preference of the fruit fly for food.

[0003] The existing operation is to cut off the front end of a 200ul syringe, and the fruit fly body is fixed in the syringe, and the head is exposed on the syringe, but the operation process is complicated, and errors are prone to occur, which leads to problems such as fruit fly escape or multiple fixation affecting fruit fly activity, fruit fly light-avoiding quiet time, etc., and if the fruit fly after the experiment is used for future experiments, the fruit fly needs to be taken out from the above fixing mode, which needs to cut open the syringe or push the fruit fly out from the bottom, and the fruit fly is extremely easy to be injured, which will seriously affect the experiment. SUMMARY

[0004] Therefore, the present application aims to provide a fruit fly proboscis extension reflex experiment fixing and air suction device and an experimental method thereof, which has the advantages of easy operation control and observation, stable experimental conditions, reduced fruit fly injury, improved experimental efficiency, etc.

[0005] To achieve the above-mentioned purpose, the following technical scheme is adopted: a fruit fly proboscis extension reflex experiment fixing and air suction device, comprising a fixing device and an air suction device, the fixing device is connected with the air suction device, the fixing device comprises a fixing device body, a fixing device top outlet, a sponge barb strip and a fixing device bottom inlet, the upper and lower parts of the fixing device body are respectively the fixing device top outlet and the fixing device bottom inlet, the fixing device body is made of sponge sheet material, a plurality of sponge barb strips are arranged on the inner wall of the fixing device body, the air suction device comprises an air suction device body, an air suction device top, a partition, a small hole, a concave hemisphere and an air suction device bottom, the upper and lower parts of the air suction device body are respectively the air suction device top and the air suction device bottom, the air suction device body is provided with a partition, the partition is a convex partition, the top of the convex partition is a concave hemisphere, and a plurality of small holes are arranged on the concave hemisphere.

[0006] Further, the fixing device bottom inlet and the fixing device top outlet are both circular, and the diameter of the fixing device bottom inlet is greater than the diameter of the fixing device top outlet.

[0007] Further, the diameter of the fixing device bottom inlet is 6mm, and the diameter of the fixing device top outlet is 3mm.

[0008] Further, the top of the inhalation device is connected with the top outlet of the fixing device.

[0009] Further, the body of the inhalation device is cylindrical.

[0010] Further, the diameter of the top and bottom of the inhalation device is 5mm.

[0011] Further, the body of the inhalation device is made of transparent polypropylene material.

[0012] Further, the bottom of the inhalation device is connected with the power source.

[0013] Further, the diameter of the concave hemisphere is 2mm.

[0014] An experimental method for fixing and inhaling device by using Drosophila proboscis extension reflex experiment, specifically comprising the following steps:

[0015] Step one, put the head of the fruit fly upwards into the bottom entrance of the fixing device, place the top outlet of the fixing device at the top of the inhalation device, turn on the power source switch of the inhalation device, and observe the position of the fruit fly in the fixing device, when the head of the fruit fly just fixes in the fixing device, turn off the power source switch, put the fixing device with fruit flies into a quiet and light-avoiding place for 30-60min for proboscis extension reflex experiment;

[0016] Step two, after the adaptation period of 30-60min in a quiet and light-avoiding place, provide water to the fruit fly and allow it to drink freely until it is satisfied, the fruit fly which does not stop reacting to water within 5min is discarded;

[0017] Step three, immerse the cotton swab with a sharp head in the corresponding test solution, then manually apply the cotton swab with the test solution to the tip of the long nose of the fruit fly for 1-2s, and monitor the long nose extension reflex, use three values to score the proboscis extension reflex test, 1.0 indicates that the fruit fly extends the long nose and feeds for 1.0s after smelling the immersed cotton swab, if the time of the fly extending the beak and consuming food is less than 1.0s, the score is 0.5, if the fruit fly fails to extend its beak, the score is 0, each fruit fly is subjected to 3 consecutive proboscis extension reflex test, with an interval of 1min, only allow the fruit fly to eat for 2s during each feeding period, in order to eliminate the slow reacting animals, provide 1% sucrose solution 3-5min after completing the test, the fruit fly which does not show proboscis extension reflex will not be included in the data set, the proboscis extension reflex index of each test is calculated as: the total score sum / test fruit fly number*100%, finally, the data is counted and calculated.

[0018] Compared with the prior art, the fruit fly proboscis extension reflex experiment fixing and inhalation device and the experimental method thereof have the following beneficial effects:

[0019] The present application is simple and convenient to operate, reduces uncertain influencing factors, and greatly improves experimental efficiency; the device is easy to make and has abundant raw material sources. BRIEF DESCRIPTION OF DRAWINGS

[0020] The accompanying drawings, which form a part of this application, are intended to provide further understanding of the application and are incorporated herein for a description of the application. The present application is shown schematically in the drawings and is explained in detail below.

[0021] Figure 1 A structural schematic view of a new type of fruit fly proboscis extension reflex experiment fixing device provided by the embodiment of the present application is shown in the figure.

[0022] Figure 2 An exploded schematic view of the new type of fruit fly proboscis extension reflex experiment fixing device is shown in the figure.

[0023] Figure 3 A structural schematic view of a new type of fruit fly proboscis extension reflex experiment fixing device provided by the embodiment of the present application is shown in the figure.

[0024] Figure 4 A combination schematic view of the new type of fruit fly proboscis extension reflex experiment fixing device and the new type of air suction device is shown in the figure.

[0025] The figure number explanation: 1, top outlet of the fixing device; 2, sponge bar with barbs; 3, bottom inlet of the fixing device; 4, sponge sheet; 5, top of the air suction device; 6, small hole; 7, concave hemisphere; 8, bottom of the air suction device. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict, and the described embodiments are only a part of the embodiments of the present application, but not all the embodiments.

[0027] Reference Figures 1-4 To illustrate the present embodiment, a fruit fly proboscis extension reflex experiment fixing and air suction device is provided, which is especially suitable for fruit fly proboscis extension reflex experiment and is composed of two parts. One part is a fixing device made of four-sided odorless sponge material, and the other part is an air suction device made of transparent polypropylene material. The shape of the fixing device is conical, and the shape of the air suction device is cylindrical. The suction head of the air suction device is concave downward to form a hemisphere, which can just cover the head of the fruit fly. The concave hemisphere 7 has a small hole 6 to prevent the fruit fly from being sucked into the device, as shown in the figure. Figure 4

[0028] ​Specifically, the fruit fly ecdysial reflex experiment fixing and suction device comprises a fixing device and a suction device, the fixing device is connected with the suction device, the fixing device comprises a fixing device body, a fixing device top outlet 1, a sponge barb strip 2 and a fixing device bottom inlet 3, the upper and lower parts of the fixing device body are the fixing device top outlet 1 and the fixing device bottom inlet 3 respectively, the fixing device body is made of a sponge sheet 4, a plurality of sponge barb strips 2 are arranged on the inner wall of the fixing device body, the suction device comprises a suction device body, a suction device top 5, a partition, a small hole 6, a concave hemisphere 7 and a suction device bottom 8, the upper and lower parts of the suction device body are the suction device top 5 and the suction device bottom 8 respectively, the partition is arranged in the suction device body, the partition is a convex partition, the top of the convex partition is the concave hemisphere 7, and a plurality of small holes 6 are arranged on the concave hemisphere 7.

[0029] The fixing device bottom inlet 3 and the fixing device top outlet 1 are both circular, and the diameter of the fixing device bottom inlet 3 is greater than that of the fixing device top outlet 1.

[0030] The diameter of the fixing device bottom inlet 3 is 6 mm, and the diameter of the fixing device top outlet 1 is 3 mm.

[0031] The suction device top 5 is connected with the fixing device top outlet 1.

[0032] The suction device body is cylindrical.

[0033] The diameters of the suction device top 5 and the suction device bottom 8 are 5 mm.

[0034] The suction device body is made of transparent polypropylene.

[0035] The suction device bottom 8 is connected with a power source.

[0036] The diameter of the concave hemisphere 7 is 2 mm.

[0037] The specific experimental method of the fruit fly ecdysial reflex experiment fixing and suction device is as follows:

[0038] 1. Put the fruit fly head upwards into the fixing device bottom inlet 3, connect the suction device top 5 with the fixing device top outlet 1 for a short time, open the power source (negative pressure suction device) switch of the suction device, and observe the position of the fruit fly in the fixing device, since the materials are transparent and visible, the concave hemisphere 7 of the suction device can cover the fruit fly head, and only the head can be exposed, and only the fruit fly head can be sucked out during suction, when the fruit fly head is exposed and fixed in the fixing device, close the negative pressure suction device power source switch, put the fixing device with the fruit fly into a quiet and light-proof place for 30-60 min, and use it for ecdysial reflex experiment.

[0039] 2. After a 30-60 min acclimation period in a quiet, light-free room, flies are offered water and allowed to drink freely until satiated. Flies that do not cease responding to water within 5 min are discarded. A cotton swab with a pointed tip is dipped into the appropriate test solution and then the test solution-coated swab is manually applied to the tip of the fly's proboscis for 1-2 s and the proboscis extension reflex is monitored. The proboscis extension reflex assay is scored using three values. A score of 1.0 indicates that the fly extends its proboscis and feeds for 1.0 s upon smelling the dipped swab. A score of 0.5 is given if the fly extends its proboscis and consumes the food for less than 1.0 s. A score of 0 is given if the fly fails to extend its proboscis. Each fly is subjected to 3 consecutive proboscis extension reflex assays, with 1 min between assays. During each feeding session, flies are allowed to feed for only 2 s. To eliminate sluggish animals, a 1% sucrose solution is offered 3-5 min after the completion of the test, and flies that do not exhibit a proboscis extension reflex are not included in the data set. The proboscis extension reflex index for each trial is calculated as: (sum of scores) / (number of flies tested) x 100%. Finally, the data is tabulated and analyzed.

[0040] The working principle of the fly proboscis extension reflex assay fixation and suction device according to the present application is as follows:

[0041] The fly head is placed into the bottom entrance 3 of the fixation device with the head facing upwards, the bottom 8 of the suction device is connected to a suction power source through a conduit, and then the top 5 of the suction device is connected to the top exit 1 of the fixation device. The fly is pushed to move upwards by the negative pressure generated by suction, and the fixation device is composed of a movable sponge with barbs, so that the fly is difficult to return after moving forward. In addition, the suction head of the suction device has a concave hemisphere 7, the size of which is just right to wrap the fly head and suck it out. The lower half of the fly's body cannot be sucked out and is limited in the fixation device, and the fly is fixed in the appropriate position of the fixation device by observing it with the naked eye.

[0042] The above disclosed embodiments of the present application are only used to help explain the present application. The embodiments do not describe all the details, nor limit the present application to the specific embodiments described. According to the content of the present specification, many modifications and changes can be made. The present specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present application, so that those skilled in the art can well understand and utilize the present application.

Claims

1. An experimental method for fixing and sucking a device using a Drosophila proboscis reflex experiment, characterized in that: The fruit fly kiss reflex experiment fixing and inhaling device comprises a fixing device and an inhaling device, the fixing device is connected with the inhaling device, the fixing device comprises a fixing device body, a fixing device top outlet (1), a sponge barb strip (2) and a fixing device bottom inlet (3), the upper and lower parts of the fixing device body are respectively the fixing device top outlet (1) and the fixing device bottom inlet (3), the fixing device body is made of a sponge sheet (4), a plurality of sponge barb strips (2) are arranged on the inner wall of the fixing device body, the inhaling device comprises an inhaling device body, an inhaling device top (5), a partition, a small hole (6), a concave hemisphere (7) and an inhaling device bottom (8), the upper and lower parts of the inhaling device body are respectively the inhaling device top (5) and the inhaling device bottom (8), the partition is arranged in the inhaling device body, the partition is a convex partition, the top of the convex partition is the concave hemisphere (7), a plurality of small holes (6) are arranged on the concave hemisphere (7); Specifically comprising the following steps: Step one, place the fruit fly head up into the fixing device bottom inlet (3), place the fixing device top outlet (1) on the inhaling device top (5), turn on the power source switch of the inhaling device, and observe the position of the fruit fly in the fixing device, when the fruit fly head just protrudes and is fixed in the fixing device, turn off the power source switch, place the fixing device with the fruit fly in a quiet and light-proof place for 30-60 min for kiss reflex experiment; Step two, after the 30-60 min adaptation period in a quiet and light-proof place, provide water for the fruit fly and allow it to drink freely until it is satisfied, the fruit fly that does not stop reacting to water within 5 min is discarded; Step three, immerse a cotton swab with a sharp tip in the corresponding test solution, then manually apply the cotton swab with the test solution to the tip of the fruit fly proboscis for 1-2 s, and monitor the proboscis extension reflex, use three values to score the kiss reflex test, 1.0 indicates that the fruit fly sniffs the soaked cotton swab and extends the proboscis and feeds for 1.0 s, if the time for the fly to extend the proboscis and consume food is less than 1.0 s, the score is 0.5, if the fruit fly fails to extend its proboscis, the score is 0, each fruit fly is subjected to 3 consecutive PER tests with an interval of 1 min, only allow the fruit fly to feed for 2 s during each feeding period, in order to eliminate sluggish animals, provide a 1% sucrose solution 3-5 min after completing the test, fruit flies that do not exhibit kiss reflex will not be included in the data set, the kiss reflex index of each test is calculated as: the total score sum / tested fruit fly number*100%, finally, the data is counted and calculated.

2. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The fixing device bottom inlet (3) and the fixing device top outlet (1) are both circular, and the diameter of the fixing device bottom inlet (3) is greater than that of the fixing device top outlet (1).

3. The experimental method of claim 2, wherein the experimental method is characterized by: The diameter of the fixing device bottom inlet (3) is 6 mm, and the diameter of the fixing device top outlet (1) is 3 mm.

4. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The fixing device top outlet (1) is placed on the inhaling device top (5).

5. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The inhaling device body is cylindrical.

6. The experimental method of claim 5, wherein the experimental method is characterized by: The diameter of the top of the inhaler (5) and the bottom of the inhaler (8) is 5mm.

7. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The body of the inhaler is made of transparent polypropylene material.

8. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The bottom of the inhaler (8) is connected to a power source.

9. The experimental method using Drosophila proboscis extension reflex experiment fixation and suction device according to claim 1, characterized in that: The diameter of the concave hemisphere (7) is 2mm.

Images