A fermentation and purification device for tryptophan production

Abstract

The invention relates to the technical field of tryptophan production, and in particular to a fermentation and purification device for tryptophan production, comprising a box body; a feed pipe is fixedly connected to the top end of the outer wall of the box body; a discharge pipe is fixedly connected to the bottom end of the outer wall of the box body, and both the discharge pipe and the feed pipe are connected to the box body; the invention drives a rotating shaft to rotate by a motor, and the rotation of the rotating shaft drives a third bevel gear to rotate, and the rotation of the third bevel gear drives a second bevel gear, a rotating column, a rotating rod and an auxiliary rod to rotate, so that the rotating rod and the auxiliary rod stir and mix the tryptophan for fermentation, and after the tryptophan fermentation is completed, the fermented tryptophan is dropped into a centrifugal filter barrel by a closing member, the centrifugal filter barrel is rotated by a transmission member to separate the fermented tryptophan, and then the tryptophan is evaporated and purified by a heating device, and the entire fermentation and purification process of the tryptophan is completed in the device, so that the tryptophan does not contact the outside world, thereby ensuring the purity and quality of the tryptophan.

Description

Technical Field

[0001] The invention relates to the technical field of tryptophan production, in particular to a fermentation and purification device for tryptophan production. Background Art

[0002] Tryptophan is an organic compound and an important amino acid. It is often used in medicine, food, and health products. In the production process of tryptophan, in order to reduce its cost, microorganisms are used for fermentation during its production, thereby achieving high yield and low-cost production.

[0003] In the prior art, when tryptophan is produced, tryptophan is usually fermented first, the fermented tryptophan is taken out, the microbial cells and the culture medium are separated by centrifugation or the like to obtain a fermentation liquid containing tryptophan, and finally the tryptophan is purified. Since the production of tryptophan requires the cooperation of multiple machines, it needs to be placed and taken out many times during the production process, which affects the work efficiency. In addition, the multiple taking and placing processes can easily lead to the contamination of tryptophan, which can easily reduce its yield and purity. Summary of the invention

[0004] The purpose of the present invention is to solve the problem that in the current tryptophan production, tryptophan is mostly fermented first, then the fermented tryptophan is taken out, and then the microbial cells and the culture medium are separated by centrifugation and the like to obtain a fermentation liquid containing tryptophan, and finally the tryptophan is purified. Since the production of tryptophan requires the cooperation of multiple machines, it is necessary to perform multiple placement and taking operations during the production process, which affects the work efficiency, and the multiple taking and placing processes are prone to cause the tryptophan to be contaminated, so that the yield and purity are easily reduced. A fermentation and purification device for tryptophan production is provided.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A fermentation and purification device for tryptophan production, comprising a box; a feed pipe is fixedly connected to the top of the outer wall of the box; a discharge pipe is fixedly connected to the bottom of the outer wall of the box, and both the discharge pipe and the feed pipe are connected to the box; a column is fixedly connected to the bottom of the outer wall of the box; a placement plate is fixedly connected to the inner wall of the box; a closing member is provided at the top of the outer wall of the placement plate; the closing member includes a closing plate; a centrifugal filter barrel is rotatably connected to the center of the bottom end of the inner wall of the box through a first cylinder; a transmission member is provided on the outer wall of the centrifugal filter barrel through a conical rack; the transmission member includes a first bevel gear; a heating device is fixedly connected to the bottom end of the inner wall of the box The outer wall of the box body is fixedly connected with an air outlet pipe at the bottom end, and the air outlet pipe is communicated with the box body; a rotating column is rotatably connected to the center of the top end of the inner wall of the box body; the outer wall of the rotating column is provided with a group of rotating rods; the outer wall of the rotating rod is fixedly connected with a group of auxiliary rods through a square block; the outer wall of the rotating column is fixedly connected with a second bevel gear; the outer wall of the box body is fixedly connected with a motor through a fixed block; the output end of the motor is provided with a rotating shaft, and one end of the outer wall of the rotating shaft passes through the outer wall of the box body and is located in the box body; one end of the outer wall of the rotating shaft passing through the box body is fixedly connected with a third bevel gear, and the third bevel gear and the second bevel gear are meshed with each other.

[0007] As a preferred embodiment of the present invention, a square through groove is opened at the center of the top end of the outer wall of the placing plate; first through grooves are opened on opposite sides of the inner wall of the square through groove; the outer walls of a pair of closing plates are respectively slidably connected to the inner walls of a pair of first through grooves; a first threaded rod is rotatably connected to one side of the outer wall of the closing plate; and one end of the outer wall of the first threaded rod passes through the box body, and the first threaded rod is threadedly connected to the box body.

[0008] As a preferred embodiment of the present invention, the inner wall of the conical rack is fixedly connected to the outer wall of the centrifugal filter barrel, and the conical rack is ring-shaped; the outer wall of the box body is rotatably connected to the first rotating rod, and one end of the outer wall of the first rotating rod passes through the outer wall of the box body and is located in the box body; one end of the outer wall of the first bevel gear is fixedly connected to one end of the outer wall of the first rotating rod located in the box body, and the first bevel gear and the conical rack are meshed with each other; the end of the outer wall of the first rotating rod that does not pass through the box body and the outer wall of the rotating shaft are both fixedly connected to the first sprocket, and a pair of first sprockets are connected by a first chain.

[0009] As a preferred embodiment of the present invention, a circular through groove is provided at the top end of the outer wall of the rotating column; a fourth bevel gear is fixedly connected to the center of the top end of the inner wall of the box body through a fixing rod, and the fourth bevel gear is located in the circular through groove; one end of the outer wall of a group of rotating rods passes through the outer wall of the rotating column and is located in the circular through groove, and a group of rotating rods are rotatably connected to the rotating shaft; a group of rotating rods are fixedly connected to one end of the outer wall located in the circular through groove, and a group of fifth bevel gears are meshed with the fourth bevel gear.

[0010] As a preferred embodiment of the present invention, a sealing plate is fixedly connected to the inner side wall of the box body, and the bottom end of the outer wall of the sealing plate is in contact with the top end of the outer wall of the centrifugal filter barrel; a second through groove is provided at the top end of the outer wall of the sealing plate; the bottom ends of the outer walls of a pair of closing plates are respectively fixedly connected to a first square plate and a second square plate through a pair of first round rods, and the bottom ends of the outer walls of the first square plate and the second square plate are both slidably connected to the top end of the outer wall of the sealing plate; the outer walls of the first square plate and the second square plate are provided with inclined surfaces on opposite sides.

[0011] As a preferred embodiment of the present invention, the top end of the outer wall of the sealing plate is rotatably connected to the sixth gear through a second round rod; a group of gear grooves are opened on one side of the outer wall of the first square plate, and the gear grooves are meshed with the sixth gear; a first gear bar is fixedly connected to one side of the outer wall of the second square plate, and the first gear bar is meshed with the sixth gear.

[0012] As a preferred embodiment of the present invention, the outer wall of the rotating column is fixedly connected to a square shell; the inner wall of the square shell is slidably connected to a sliding block; one end of the outer wall of the sliding block is fixedly connected to a cleaning plate through a spring, and the outer wall of one end of the cleaning plate is slidably connected to the inner wall of the square shell, and one side of the outer wall of the cleaning plate is in contact with the inner wall of the box body.

[0013] As a preferred embodiment of the present invention, a thread groove is provided at one end of the outer wall of the sliding block; a second threaded rod is threadedly connected to the inner wall of the thread groove, and one end of the outer wall of the second threaded rod is rotatably connected to the outer wall of the rotating column; a seventh bevel gear is fixedly connected to the outer wall of the second threaded rod; a third rotating rod is rotatably connected to one side of the outer wall of the square shell, and one end of the outer wall of the third rotating rod passes through the square shell and is located inside the square shell; an eighth bevel gear is fixedly connected to one end of the outer wall of the third rotating rod passing through the square shell, and the eighth bevel gear and the seventh bevel gear are meshed with each other.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The motor drives the rotating shaft to rotate, and the rotation of the rotating shaft drives the first rotating rod and the first bevel gear to rotate through a pair of first sprockets and a first chain, and the first bevel gear drives the conical rack to rotate, and the rotation of the conical rack drives the centrifugal filter barrel to rotate, so that the fermented tryptophan generates centrifugal force in the rotating centrifugal filter barrel, so that impurities and unnecessary substances such as microbial cells in the tryptophan fermentation liquid can be separated, so that the purity of the tryptophan fermentation liquid is higher.

[0016] 2. The cleaning plate is driven to rotate by the rotating column, so that the inner wall of the box is cleaned by the rotation of the cleaning plate. When the tryptophan fermentation liquid is stained on the inner wall of the box, the rotation of the cleaning plate will clean it, so that the tryptophan fermentation liquid is not easily wasted. Therefore, the device can not only prevent the waste of tryptophan fermentation liquid through the cleaning plate, but also prevent the liquid from being stained on the inner wall of the box, resulting in insufficient fermentation agent required for tryptophan. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to facilitate understanding by those skilled in the art, the present invention is further described below with reference to the accompanying drawings.

[0018] Figure 1 It is the main structure diagram of the present invention;

[0019] Figure 2 It is a cross-sectional structural diagram of the box body of the present invention;

[0020] Figure 3 It is the internal structure diagram of the box of the present invention;

[0021] Figure 4 It is a structural diagram of the rotating rod and the cleaning plate of the present invention;

[0022] Figure 5 It is a cross-sectional structural diagram of the rotating column of the present invention;

[0023] Figure 6 It is an exploded structural diagram of the square shell, the sliding block and the cleaning plate of the present invention;

[0024] Figure 7 It is an exploded structural diagram of the placement plate and the sealing plate of the present invention;

[0025] Figure 8 It is an exploded structural diagram of the sealing plate and the centrifugal filter barrel of the present invention;

[0026] Fig. 9 It is an exploded structural diagram of the sealing plate, the first square plate and the second square plate of the present invention;

[0027] Fig.10 It is a structural diagram of the centrifugal filter barrel and the rotating shaft of the present invention;

[0028] In the figure: 1, box body; 2, feed pipe; 3, discharge pipe; 4, placement plate; 5, closing plate; 6, centrifugal filter barrel; 7, conical rack; 8, first bevel gear; 9, heating device; 10, air outlet pipe; 11, rotating column; 12, rotating rod; 13, auxiliary rod; 14, second bevel gear; 15, motor; 16, rotating shaft; 17, third bevel gear; 18, square through groove; 19, first through groove; 20, first threaded rod; 21, first rotating rod; 22, first chain Wheel; 23, first chain; 24, circular through groove; 25, fourth bevel gear; 26, fifth bevel gear; 27, sealing plate; 28, second through groove; 29, first square plate; 30, second square plate; 31, sixth gear; 32, gear groove; 33, first gear bar; 34, square shell; 35, sliding block; 36, cleaning plate; 37, threaded groove; 38, second threaded rod; 39, seventh bevel gear; 40, third rotating rod; 41, eighth bevel gear; 42, column. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Embodiment 1:

[0031] See also Figure 1-Figure 6As shown, a fermentation and purification device for tryptophan production comprises a box body 1; a feed pipe 2 is fixedly connected to the top of the outer wall of the box body 1; a discharge pipe 3 is fixedly connected to the bottom of the outer wall of the box body 1, and the discharge pipe 3 and the feed pipe 2 are both connected to the box body 1; a column 42 is fixedly connected to the bottom of the outer wall of the box body 1; a placement plate 4 is fixedly connected to the inner wall of the box body 1; a closing member is provided at the top of the outer wall of the placement plate 4; the closing member comprises a closing plate 5; a centrifugal filter barrel 6 is rotatably connected to the center of the bottom end of the inner wall of the box body 1 through a first cylinder; a transmission member is provided on the outer wall of the centrifugal filter barrel 6 through a conical rack 7; the transmission member comprises a first bevel gear 8; a heating device 9 is fixedly connected to the bottom end of the inner wall of the box body 1; an air outlet pipe 10 is fixedly connected to the bottom end of the outer wall of the box body 1, and the air outlet pipe 10 is connected to the box body. 1 is connected; a rotating column 11 is rotatably connected at the center of the top of the inner wall of the box body 1; a group of rotating rods 12 are provided on the outer wall of the rotating column 11; a group of auxiliary rods 13 are fixedly connected to the outer wall of the rotating rod 12 through a square block; a second bevel gear 14 is fixedly connected to the outer wall of the rotating column 11; a motor 15 is fixedly connected to the outer wall of the box body 1 through a fixed block; a rotating shaft 16 is provided at the output end of the motor 15, and one end of the outer wall of the rotating shaft 16 passes through the outer wall of the box body 1 and is located in the box body 1; a third bevel gear 17 is fixedly connected to one end of the outer wall of the rotating shaft 16 passing through the box body 1, and the third bevel gear 17 is meshed with the second bevel gear 14. By putting tryptophan and fermentation agents into the box body 1 through the feeding pipe 2, and then closing the feeding pipe 2 through the valve, the passage The motor 15 drives the rotating shaft 16 to rotate. Since the rotating shaft 16 passes through the outer wall of the box body 1 and is fixedly connected to a third bevel gear 17 at one end, and the third bevel gear 17 is meshed with the second bevel gear 14 and the outer side wall of the rotating column 11 is fixedly connected to the second bevel gear 14, the rotation of the rotating shaft 16 drives the third bevel gear 17 to rotate, and the rotation of the third bevel gear 17 drives the second bevel gear 14 and the rotating column 11 to rotate, so that the rotating column 11 drives a group of rotating rods 12 and auxiliary rods 13 to rotate, so that the rotation of the rotating rods 12 and the auxiliary rods 13 stirs the tryptophan and the fermentation agent to mix them evenly and ferment them. When the tryptophan fermentation is completed, the placement plate 4 is opened through the closing member to allow the fermented tryptophan to fall into the centrifugal filter barrel 6 , thereby the centrifugal filter barrel 6 is rotated through the transmission member, so that the fermented tryptophan is separated, and then the tryptophan is heated and evaporated by the heating device 9, the heating device 9 is a prior art and is not described, so that the evaporated gas is discharged from the outlet pipe 10, and when the tryptophan is evaporated and purified, the discharge pipe 3 is opened to take out the fermented and purified tryptophan, so that the device does not need to use multiple machines for fermentation and purification when the tryptophan is fermented and purified, thereby saving the transfer and placement time of tryptophan, thereby improving work efficiency, and the entire fermentation and purification process of tryptophan is completed in the device, so that the tryptophan does not contact with the outside world, so that it is not easily contaminated by the outside world, thereby ensuring the purity and quality of the tryptophan.

[0032] A circular through groove 24 is provided at the top of the outer wall of the rotating column 11; a fourth bevel gear 25 is fixedly connected to the center of the top of the inner wall of the box body 1 through a fixed rod, and the fourth bevel gear 25 is located in the circular through groove 24; one end of the outer wall of a group of rotating rods 12 passes through the outer wall of the rotating column 11 and is located in the circular through groove 24, and a group of rotating rods 12 are rotatably connected to the rotating shaft 16; a group of rotating rods 12 are fixedly connected to a fifth bevel gear 26 at one end of the outer wall located in the circular through groove 24, and a group of fifth bevel gears 26 are meshed with the fourth bevel gear 25, and the rotating rod 12 is driven to rotate by the rotating column 11, so that the rotating rod 12 and the auxiliary rod 13 rotate with the rotating column 11, because the fourth bevel gear 25 is fixedly connected to the center of the top of the inner wall of the box body 1 through a fixed rod, and the fourth bevel gear 25 is located in the circular through groove 24 and a group of rotating rods 12 are fixedly connected to a fifth bevel gear 26 at one end of the outer wall located in the circular through groove 24, and A set of fifth bevel gears 26 are meshed with the fourth bevel gear 25, so that when the rotating rod 12 rotates with the rotating column 11, the rotating rod 12 will also rotate with the fifth bevel gear 26, while the fourth bevel gear 25 is stationary, so that when the fifth bevel gear 26 rotates with the rotating column 11, it will rotate along the fourth bevel gear 25, so that the fifth bevel gear 26 drives the rotating rod 12 to rotate, so that the rotating rod 12 and the auxiliary rod 13 rotate while rotating with the rotating column 11, thereby enhancing the stirring effect of the device and improving the stirring efficiency of the device, and because the fifth bevel gear 26 and the fourth bevel gear 25 are both located in the circular through groove 24, the tryptophan fermentation liquid will not contact the fifth bevel gear 26 and the fourth bevel gear 25, so that the tryptophan is not easily wasted and is not easily contacted with other devices, thereby ensuring the safety of the device while preventing the tryptophan from being contaminated.

[0033] The outer wall of the rotating column 11 is fixedly connected to a square shell 34; the inner wall of the square shell 34 is slidably connected to a sliding block 35; one end of the outer wall of the sliding block 35 is fixedly connected to a cleaning plate 36 through a spring, and the outer wall of one end of the cleaning plate 36 is slidably connected to the inner wall of the square shell 34, and one side of the outer wall of the cleaning plate 36 is in contact with the inner wall of the box body 1, and the cleaning plate 36 is fixedly connected to the outer wall of the sliding block 35 through a spring, and the outer wall of one end of the cleaning plate 36 is slidably connected to the inner wall of the square shell 34, and one side of the outer wall of the cleaning plate 36 is in contact with the inner wall of the box body 1, so that when the rotating column 11 rotates, it will drive the cleaning plate 36 to rotate, thereby making the cleaning plate 36 6 will clean the inner wall of the box body 1 by rotation, so that when the tryptophan fermentation liquid is stained on the inner wall of the box body 1, the rotation of the cleaning plate 36 will clean it and make it fall onto the placement plate 4 to mix with other tryptophan fermentation liquids, so that the tryptophan fermentation liquid is not easily wasted, and the tryptophan and the fermentation agent will not be stained on the inner wall of the box body 1 due to tryptophan or part of the fermentation agent, thereby causing incomplete fermentation of part of the tryptophan. Therefore, the present device can not only prevent the waste of tryptophan fermentation liquid through the cleaning plate 36, but also prevent the liquid from being stained on the inner wall of the box body 1, resulting in insufficient fermentation agent required for tryptophan.

[0034] A thread groove 37 is provided at one end of the outer wall of the sliding block 35; a second threaded rod 38 is threadedly connected to the inner side wall of the thread groove 37, and one end of the outer wall of the second threaded rod 38 is rotatably connected to the outer side wall of the rotating column 11; a seventh bevel gear 39 is fixedly connected to the outer side wall of the second threaded rod 38; a third rotating rod 40 is rotatably connected to one side of the outer wall of the square shell 34, and one end of the outer wall of the third rotating rod 40 passes through the square shell 34 and is located inside the square shell 34; an eighth bevel gear 41 is fixedly connected to one end of the outer wall of the third rotating rod 40 that passes through the square shell 34, and the eighth bevel gear 41 and the seventh bevel gear 39 are meshed with each other, and the rotation of the third rotating rod 40 will cause the third rotating rod 40 to drive the eighth bevel gear 41 to rotate, thereby causing the rotation of the eighth bevel gear 41 to drive the seventh bevel gear 39 to rotate, thereby causing the seventh bevel gear 39 to drive the second threaded rod 38 to rotate, because the inner side wall of the thread groove 37 is threadedly connected to the second threaded rod 38, the rotation of the second threaded rod 38 The sliding block 35 will be driven to move, so that the sliding block 35 drives the spring and the cleaning plate 36 to move, so that the cleaning plate 36 can work for a long time. When it is worn, the sliding block 35 can be driven to move the spring and the cleaning plate 36 by rotating the third rotating rod 40, so that the cleaning plate 36 can be re-attached to the inner wall of the box body 1 to clean the inner wall of the box body 1, and the sliding block 35 and the cleaning plate 36 are connected by a spring, so that the cleaning plate 36 has elasticity, so that when the cleaning plate 36 is less worn, the elastic force of the spring can squeeze the cleaning plate 36 so that it always fits the inner wall of the box body 1, thereby ensuring that the cleaning plate 36 can always maintain a cleaning state. When it is worn too much or the elastic force of the spring is weak after working for a long time, the sliding block 35 can be driven to move by the third rotating rod 40, so that the cleaning plate 36 is closer to the inner wall of the box body 1, and the elastic force of the spring can be adjusted by the movement of the sliding block 35, so that the spring has sufficient elastic force.

[0035] Embodiment 2:

[0036] See also Figure 2-3 and Figure 7-10As shown, a square through groove 18 is provided at the center of the top of the outer wall of the placing plate 4; first through grooves 19 are provided on opposite sides of the inner wall of the square through groove 18; the outer side walls of a pair of closing plates 5 are slidably connected to the inner side walls of a pair of first through grooves 19 respectively; a first threaded rod 20 is rotatably connected to one side of the outer wall of the closing plate 5, and one end of the outer wall of the first threaded rod 20 passes through the box body 1, and the first threaded rod 20 is threadedly connected to the box body 1, and by rotating the first threaded rod 20, because the outer wall of the closing plate 5 is rotatably connected to the first threaded rod 20 on one side, and the first threaded rod 20 passes through the box body 1, the first threaded rod 20 is threadedly connected to the box body 1 The first threaded rod 20 is connected so that when the first threaded rod 20 rotates, the first threaded rod 20 will move, so that the movement of the first threaded rod 20 drives the closing plate 5 to move, so that the movement of the closing plate 5 opens the square through groove 18 on the placement plate 4, so that tryptophan can fall from the square through groove 18, and after the tryptophan falls into the centrifugal filter barrel 6, the first threaded rod 20 is reversed to make the closing plate 5 close the square through groove 18, so that the device can operate the tryptophan outside the box body 1 to enter the next step of operation, so that the tryptophan will not be exposed to the outside world during fermentation and purification, thereby ensuring its purity and preventing it from being contaminated.

[0037] The inner side wall of the box body 1 is fixedly connected with a sealing plate 27, and the bottom end of the outer wall of the sealing plate 27 is in contact with the top end of the outer wall of the centrifugal filter barrel 6; the top end of the outer wall of the sealing plate 27 is provided with a second through groove 28; the bottom ends of the outer walls of a pair of closing plates 5 are respectively fixedly connected with a first square plate 29 and a second square plate 30 through a pair of first round rods, and the bottom ends of the outer walls of the first square plate 29 and the second square plate 30 are both slidably connected to the top end of the outer wall of the sealing plate 27; the outer walls of the first square plate 29 and the second square plate 30 are both provided with inclined surfaces on opposite sides. When the closing plate 5 is opened, the movement of the closing plate 5 will drive the first square plate 29 to move through the first round rod, so that the movement of the first square plate 29 opens the second through groove 28, so that the tryptophan fermentation liquid can flow from the square through groove 18 When the centrifugal filter barrel 6 falls, the tryptophan fermentation liquid will fall into the centrifugal filter barrel 6 through the second through groove 28 and be centrifugally separated, and when the centrifugal filter barrel 6 is not rotating, the first threaded rod 20 is reversed to make the closing plate 5 drive the first square plate 29 to move, so that the first square plate 29 blocks the second through groove 28 again, and the centrifugal filter barrel 6 rotates at this time, so that when the tryptophan fermentation liquid is centrifugally separated, the top of the centrifugal filter barrel 6 will be blocked by the sealing plate 27 and the first square plate 29, so that when the tryptophan fermentation liquid is centrifugally separated, the liquid and impurities of the tryptophan fermentation liquid will be blocked by the sealing plate 27 and the first square plate 29, so that the tryptophan fermentation liquid and impurities will not splash out of the centrifugal filter barrel 6, thereby ensuring the purity of the separated tryptophan fermentation liquid.

[0038] The top of the outer wall of the sealing plate 27 is rotatably connected to the sixth gear 31 through the second round rod; a group of gear grooves 32 are opened on one side of the outer wall of the first square plate 29, and the gear grooves 32 and the sixth gear 31 are meshed with each other; a first gear bar 33 is fixedly connected to one side of the outer wall of the second square plate 30, and the first gear bar 33 and the sixth gear 31 are meshed with each other. When the first square plate 29 is driven to move by the closing plate 5, a group of gear grooves 32 are opened on one side of the outer wall of the first square plate 29, and the gear grooves 32 and the sixth gear 31 are meshed with each other, so that the movement of the first square plate 29 will drive the sixth gear 31 to rotate. Because the first gear bar 33 is fixedly connected to one side of the outer wall of the second square plate 30, and the first gear bar 33 and the sixth gear 31 are meshed with each other, the rotation of the sixth gear 31 will drive the first gear bar 33 to move, thereby The first gear bar 33 drives the second square plate 30 to move in the opposite direction, and the movement of the second square plate 30 drives the other closing plate 5 to move through the first round rod, so that when the device moves one closing plate 5, it drives the first square plate 29 and the second square plate 30 to move in the opposite direction at the same time, so that when the second through groove 28 is opened, it also drives the other closing plate 5 to move, so that the other closing plate 5 also moves in the opposite direction, so that after the tryptophan fermentation liquid is fermented, through the movement of a pair of closing plates 5 and the movement of the first square plate 29 and the second square plate 30, the tryptophan fermentation liquid can fall into the centrifugal filter barrel 6 through the larger square through groove 18 and the second through groove 28, so that the tryptophan fermentation liquid can fall to the next operation more easily, and the operation of the device is also simpler and more convenient.

[0039] The inner side wall of the conical rack 7 is fixedly connected to the outer side wall of the centrifugal filter barrel 6, and the conical rack 7 is ring-shaped; the outer side wall of the box body 1 is rotatably connected to the first rotating rod 21, and one end of the outer wall of the first rotating rod 21 passes through the outer side wall of the box body 1 and is located in the box body 1; one end of the outer wall of the first bevel gear 8 is fixedly connected to one end of the outer wall of the first rotating rod 21 located in the box body 1, and the first bevel gear 8 and the conical rack 7 are meshed with each other; one end of the outer wall of the first rotating rod 21 that does not pass through the box body 1 and the outer side wall of the rotating shaft 16 are fixedly connected with a first sprocket 22, and a pair of first sprockets 22 are connected by a first chain 23, and the rotating shaft 16 is driven to rotate by the motor 15. Because the first rotating rod 21 does not pass through the outer wall of the box body 1, one end of the outer wall of the rotating shaft 16 and the outer side wall of the rotating shaft 16 are fixedly connected with a first sprocket 22, and a pair of first sprockets 22 are connected by a first chain 23, so that the rotation of the rotating shaft 16 is achieved through a pair of first sprockets 2 2 and the first chain 23 drive the first rotating rod 21 to rotate, so that the rotation of the first rotating rod 21 drives the first bevel gear 8 to rotate. Because one end of the outer wall of the first bevel gear 8 is fixedly connected to one end of the outer wall of the first rotating rod 21 located in the box body 1, and the first bevel gear 8 is meshed with the conical rack 7, the rotation of the first bevel gear 8 will drive the conical rack 7 to rotate, so that the rotation of the conical rack 7 drives the centrifugal filter barrel 6 to rotate, so that the fermented tryptophan generates centrifugal force in the rotating centrifugal filter barrel 6, so that the fermented tryptophan is separated from the impurities of the tryptophan and the fermentation liquid containing the tryptophan under the action of the centrifugal force, and the fermentation liquid containing the tryptophan falls from the filter holes on the inner wall of the centrifugal filter barrel 6 to the bottom end of the inner wall of the box body 1, so that the impurities and unnecessary objects such as microbial cells in the tryptophan fermentation liquid can be separated, so that the purity of the tryptophan fermentation liquid is higher.

[0040] When the present invention is in use, tryptophan and fermentation agents are placed into the housing 1 through the feed pipe 2, and the feed pipe 2 is closed through the valve. At this time, the motor 15 drives the rotating shaft 16 to rotate. Since the rotating shaft 16 passes through the outer wall of the housing 1 and is fixedly connected to a third bevel gear 17 at one end, and the third bevel gear 17 is meshed with the second bevel gear 14 and the outer side wall of the rotating column 11 is fixedly connected to the second bevel gear 14, the rotation of the rotating shaft 16 drives the third bevel gear 17 to rotate, and the rotation of the third bevel gear 17 drives the second bevel gear 14 and the rotating column 11 to rotate, so that the rotating column 11 drives a group of rotating rods 12 and auxiliary rods 13 to rotate, so that the rotation of the rotating rods 12 and auxiliary rods 13 stirs the tryptophan and the fermentation agent to make them evenly mixed, thereby fermenting. , and when the rotating column 11 drives the rotating rod 12 to rotate, when the rotating rod 12 and the auxiliary rod 13 rotate with the rotating column 11, because the fourth bevel gear 25 is fixedly connected to the center of the top of the inner wall of the box body 1 through the fixing rod, and the fourth bevel gear 25 is located in the circular through groove 24 and a group of the outer wall ends of the rotating rods 12 located in the circular through groove 24 are fixedly connected with the fifth bevel gear 26, and a group of the fifth bevel gears 26 are meshed with the fourth bevel gear 25, so that when the rotating rod 12 rotates with the rotating column 11, the rotating rod 12 will drive the fifth bevel gear 26 to rotate, and the fourth bevel gear 25 is stationary, so that when the fifth bevel gear 26 rotates with the rotating column 11, it will rotate along the fourth bevel gear 25, so that the rotation of the fifth bevel gear 26 drives the rotating rod 1 2, so that the rotating rod 12 and the auxiliary rod 13 rotate at the same time as the rotating column 11 rotates, thereby enhancing the stirring effect of the device and improving the stirring efficiency of the device. In addition, the fifth bevel gear 26 and the fourth bevel gear 25 are both located in the circular through groove 24, so that the tryptophan fermentation liquid will not contact the fifth bevel gear 26 and the fourth bevel gear 25, so that the tryptophan is not easily wasted and is not easily contacted with other devices, thereby ensuring the safety of the device and preventing the tryptophan from being contaminated. When the rotating column 11 rotates, the rotation of the rotating column 11 drives the square shell 34 to rotate, so that the square shell 34 drives the cleaning plate 36 to rotate, so that the rotation of the cleaning plate 36 cleans the inner wall of the box body 1, thereby the tryptophan fermentation liquid is not easily wasted and is not easily contacted with other devices, thereby ensuring the safety of the device and preventing the tryptophan from being contaminated. When the rotating column 11 rotates, the rotation of the rotating column 11 drives the square shell 34 to rotate, so that the square shell 34 drives the cleaning plate 36 to rotate, so that the rotation of the cleaning plate 36 cleans the inner wall of the box body 1, thereby When the fermentation liquid is stained on the inner wall of the box body 1, the rotation of the cleaning plate 36 will clean it and make it fall onto the placement plate 4 to mix with other tryptophan fermentation liquid, so that the device can not only prevent the waste of tryptophan fermentation liquid through the cleaning plate 36, but also prevent the liquid from being stained on the inner wall of the box body 1, so that tryptophan will not be incompletely fermented due to insufficient required fermentation agent, and the cleaning plate 36 is connected to the sliding block 35 through a spring, so that the rotation of the third rotating rod 40 will drive the eighth bevel gear 41 to rotate, and the eighth bevel gear 41 will drive the seventh bevel gear 39 to rotate, and the seventh bevel gear 39 will drive the second threaded rod 38 to rotate. Because the inner wall of the thread groove 37 is threadedly connected with the second threaded rod 38, the rotation of the second threaded rod 38 will drive the sliding block 35 to move.The sliding block 35 drives the spring and the cleaning plate 36 to move, so that the cleaning plate 36 can work for a long time, so that when the wear is small, because the sliding block 35 and the cleaning plate 36 are connected by a spring, the cleaning plate 36 has elasticity, so that when the cleaning plate 36 is less worn, the elastic force of the spring will squeeze the cleaning plate 36, so that it always fits the inner wall of the box body 1, thereby ensuring that the cleaning plate 36 can always maintain a cleaning state. When the wear is too much or the elastic force of the spring is weak after working for a long time, the sliding block 35 can be driven to move by the third rotating rod 40, so that the cleaning plate 36 is closer to the inner wall of the box body 1, and the elastic force of the spring can be adjusted by the movement of the sliding block 35, so that the spring has sufficient elastic force, thereby extending the service life of the cleaning plate 36. When the tryptophan is evenly stirred and the fermentation is completed, by rotating the first threaded rod 20, since one side of the outer wall of the closing plate 5 is rotatably connected with the first threaded rod 20, and the first threaded rod 20 penetrates the box body 1, the first threaded rod 20 is threadedly connected to the box body 1, so that the rotation of the first threaded rod 20 will drive the first threaded rod 20 to move, so that the movement of the first threaded rod 20 drives the closing plate 5 to move, and the movement of the closing plate 5 will open the square groove 18 on the placement plate 4, so that the tryptophan fermentation liquid can fall from the square groove 18, and when the closing plate 5 is opened, the movement of the closing plate 5 will drive the first square plate 29 to move through the first round rod, so that the movement of the first square plate 29 opens the second groove 28, and when the first square plate 29 moves, due to the first square plate 29 A group of gear grooves 32 are provided on one side of the outer wall of the second square plate 30, and the gear grooves 32 mesh with the sixth gear 31, so that the movement of the first square plate 29 will drive the sixth gear 31 to rotate. Because the first gear bar 33 is fixedly connected to one side of the outer wall of the second square plate 30, and the first gear bar 33 meshes with the sixth gear 31, the rotation of the sixth gear 31 will drive the first gear bar 33 to move, so that the first gear bar 33 drives the second square plate 30 to move in the opposite direction, and the movement of the second square plate 30 will drive the other closing plate 5 to move through the first round rod, so that when the device moves one closing plate 5, it will drive the first square plate 29 and the second square plate 30 to move in the opposite direction at the same time, thereby opening the second through slot 28, and the movement of one closing plate 5 will also drive the other closing plate 5 moves, so that the closing plate 5 moves to open the square through slot 18 to make the tryptophan fermented liquid fall, which will drive the other closing plate 5 to move in the opposite direction, making it easier for the tryptophan fermented liquid to fall, and the movement of the closing plate 5 will also drive the first square plate 29 and the second square plate 30 to open the second through slot 28, so that the tryptophan fermented liquid falls into the centrifugal filter barrel 6 through the square through slot 18 and the second through slot 28, and when the closing plate 5 closes the square through slot 18, it will also drive the first square plate 29 and the second square plate 30 to close the second through slot 28, so that the top of the centrifugal filter barrel 6 is blocked by the sealing plate 27 and the first square plate 29 and the second square plate 30, and the outer walls of the first square plate 29 and the second square plate 30 are provided with inclined surfaces on opposite sides, so that when the tryptophan fermented liquid falls,Even if it is spilled on the first square plate 29 and the second square plate 30, it will slide down the inclined surface, thereby preventing the tryptophan fermentation liquid from being wasted. At this time, the rotating shaft 16 is driven to rotate by the motor 15. Because the first rotating rod 21 does not penetrate the outer wall of the box body 1, one end and the outer side wall of the rotating shaft 16 are fixedly connected with the first sprocket 22, and the pair of first sprockets 22 are connected by the first chain 23. The rotation of the rotating shaft 16 drives the first rotating rod 21 to rotate through the pair of first sprockets 22 and the first chain 23, so that the rotation of the first rotating rod 21 drives the first bevel gear 8 to rotate. Because one end of the outer wall of the first bevel gear 8 is fixed The first rotating rod 21 is connected to one end of the outer wall of the housing 1, and the first bevel gear 8 is meshed with the conical rack 7, so that the rotation of the first bevel gear 8 drives the conical rack 7 to rotate, and the rotation of the conical rack 7 drives the centrifugal filter barrel 6 to rotate, so that the fermented tryptophan impurities and the fermentation liquid containing tryptophan are separated under the action of centrifugal force, and the fermentation liquid containing tryptophan falls from the filter holes on the inner wall of the centrifugal filter barrel 6 to the bottom end of the inner wall of the housing 1, so that the tryptophan fermentation liquid can remove impurities and make it higher in purity, and the top of the centrifugal filter barrel 6 is sealed. The sealing plate 27 and the first square plate 29 and the second square plate 30 are sealed, so that the impurities in the tryptophan fermentation liquid will not splash out of the centrifugal filter barrel 6 from the top of the centrifugal filter barrel 6 during the centrifugal process, so that the impurities cannot escape from the centrifugal filter barrel 6, thereby ensuring the purity and quality of the fermentation liquid containing tryptophan, and preventing the impurities from splashing and increasing the difficulty of cleaning the box 1. When the fermentation liquid containing tryptophan falls from the filter holes on the inner wall of the centrifugal filter barrel 6 to the bottom end of the inner wall of the box 1, the fermentation liquid containing tryptophan is heated and evaporated by the heating device 9. During the evaporation and purification The gas generated during the fermentation can be discharged from the gas outlet pipe 10 by opening the gas outlet pipe 10. After the fermentation liquid containing tryptophan is evaporated and purified, the tryptophan after fermentation and purification can be taken out by opening the valve on the discharge pipe 3. This device does not need to use multiple machines for fermentation and purification when fermenting and purifying tryptophan, saving the transfer and placement time of tryptophan, improving work efficiency, and the entire fermentation and purification process of tryptophan is completed in the box 1, so that the tryptophan does not contact the outside world, so that it is not easily contaminated by the outside world, thereby ensuring the purity and quality of tryptophan.

[0041] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A fermentation and purification device for tryptophan production, comprising a box (1); a feed pipe (2) is fixedly connected to the top of the outer wall of the box (1); a discharge pipe (3) is fixedly connected to the bottom of the outer wall of the box (1), and both the discharge pipe (3) and the feed pipe (2) are connected to the box (1); a column (42) is fixedly connected to the bottom of the outer wall of the box (1); characterized in that: The inner wall of the box body (1) is fixedly connected to a placement plate (4); a closing member is provided at the top of the outer wall of the placement plate (4); the closing member includes a closing plate (5); a centrifugal filter barrel (6) is rotatably connected to the center of the bottom end of the inner wall of the box body (1) via a first cylinder; a transmission member is provided on the outer wall of the centrifugal filter barrel (6) via a conical rack (7); the transmission member includes a first bevel gear (8); a heating device (9) is fixedly connected to the bottom end of the inner wall of the box body (1); an air outlet pipe (10) is fixedly connected to the bottom end of the outer wall of the box body (1); and a rotating column (11) is rotatably connected to the center of the top end of the inner wall of the box body (1); The outer wall of the rotating column (11) is provided with a group of rotating rods (12); the outer wall of the rotating rod (12) is fixedly connected to a group of auxiliary rods (13) through a square block; the outer wall of the rotating column (11) is fixedly connected to a second bevel gear (14); the outer wall of the box body (1) is fixedly connected to a motor (15) through a fixed block; the output end of the motor (15) is provided with a rotating shaft (16), and one end of the outer wall of the rotating shaft (16) passes through the outer wall of the box body (1) and is located inside the box body (1); one end of the rotating shaft (16) passing through the outer wall of the box body (1) is fixedly connected to a third bevel gear (17) meshing with the second bevel gear (14); A square through groove (18) is provided at the center of the top end of the outer wall of the placement plate (4); first through grooves (19) are provided on opposite sides of the inner wall of the square through groove (18); the outer walls of a pair of closing plates (5) are slidably connected to the inner walls of a pair of first through grooves (19); a first threaded rod (20) is rotatably connected to one side of the outer wall of the closing plate (5); and one end of the outer wall of the first threaded rod (20) passes through the box body (1), and the first threaded rod (20) is threadedly connected to the box body (1); The inner side wall of the conical rack (7) is fixedly connected to the outer side wall of the centrifugal filter barrel (6), and the conical rack (7) is ring-shaped; the outer side wall of the box body (1) is rotatably connected to a first rotating rod (21), and one end of the outer wall of the first rotating rod (21) penetrates the outer side wall of the box body (1) and is located inside the box body (1); one end of the outer wall of the first bevel gear (8) is fixedly connected to one end of the outer wall of the first rotating rod (21) located inside the box body (1), and the first bevel gear (8) and the conical rack (7) are meshed with each other; one end of the outer wall of the first rotating rod (21) that does not penetrate the box body (1) and the outer side wall of the rotating shaft (16) are both fixedly connected to a first sprocket (22), and a pair of first sprockets (22) are connected by a first chain (23); A circular through groove (24) is formed at the top of the outer wall of the rotating column (11); a fourth bevel gear (25) is fixedly connected to the center of the top of the inner wall of the box body (1) through a fixing rod, and the fourth bevel gear (25) is located in the circular through groove (24); one end of the outer wall of a group of rotating rods (12) passes through the outer wall of the rotating column (11) and is located in the circular through groove (24), and the group of rotating rods (12) are rotatably connected to the rotating shaft (16); one end of the outer wall of a group of rotating rods (12) located in the circular through groove (24) is fixedly connected to a fifth bevel gear (26), and the group of fifth bevel gears (26) are meshed with the fourth bevel gear (25); The inner wall of the box body (1) is fixedly connected with a sealing plate (27), and the bottom end of the outer wall of the sealing plate (27) is in contact with the top end of the outer wall of the centrifugal filter barrel (6); the top end of the outer wall of the sealing plate (27) is provided with a second through groove (28); the bottom ends of the outer walls of a pair of closing plates (5) are respectively fixedly connected with a first square plate (29) and a second square plate (30) through a pair of first round rods, and the bottom ends of the outer walls of the first square plate (29) and the second square plate (30) are both slidably connected to the top end of the outer wall of the sealing plate (27); and the outer walls of the first square plate (29) and the second square plate (30) are both provided with inclined surfaces on opposite sides.

2. A fermentation and purification device for tryptophan production according to claim 1, characterized in that: The top end of the outer wall of the sealing plate (27) is rotatably connected to a sixth gear (31) via a second round rod; a group of gear grooves (32) are provided on one side of the outer wall of the first square plate (29), and the gear grooves (32) and the sixth gear (31) are meshed with each other; a first gear bar (33) is fixedly connected to one side of the outer wall of the second square plate (30), and the first gear bar (33) and the sixth gear (31) are meshed with each other.

3. A fermentation and purification device for tryptophan production according to claim 1, characterized in that: The outer wall of the rotating column (11) is fixedly connected to a square shell (34); the inner wall of the square shell (34) is slidably connected to a sliding block (35); one end of the outer wall of the sliding block (35) is fixedly connected to a cleaning plate (36) via a spring, and the outer wall of one end of the cleaning plate (36) is slidably connected to the inner wall of the square shell (34), and one side of the outer wall of the cleaning plate (36) is in contact with the inner wall of the box body (1).

4. A fermentation and purification device for tryptophan production according to claim 3, characterized in that: A thread groove (37) is formed at one end of the outer wall of the sliding block (35); a second threaded rod (38) is threadedly connected to the inner wall of the thread groove (37), and one end of the outer wall of the second threaded rod (38) is rotatably connected to the outer wall of the rotating column (11); a seventh bevel gear (39) is fixedly connected to the outer wall of the second threaded rod (38); a third rotating rod (40) is rotatably connected to one side of the outer wall of the square shell (34), and one end of the outer wall of the third rotating rod (40) passes through the square shell (34) and is located inside the square shell (34); an eighth bevel gear (41) is fixedly connected to one end of the outer wall of the third rotating rod (40) passing through the square shell (34), and the eighth bevel gear (41) and the seventh bevel gear (39) are meshed with each other.

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