Preparation and application of a traditional Chinese medicine aromatherapy spray and ointment extract with multiple activities

Abstract

This invention belongs to the field of traditional Chinese medicine, specifically relating to the preparation and application of a traditional Chinese medicine aromatherapy spray / ointment based on multiple effects. The composition of the aromatherapy is selected from at least eight of the following: peppermint, calamus, clove, patchouli, jasmine, atractylodes, artemisia, eupatorium, ligusticum, lavender, olive leaf, rose, mugwort, nutmeg, sophora japonica, honeysuckle, scutellaria, peppermint, and cinnamon. The extract of this herbal combination exhibits significant inhibitory effects on Staphylococcus aureus and Streptococcus epidermidis. Application surveys show it possesses significant pharmacological activities such as air freshening, mosquito repellent, antibacterial properties, and the ability to refresh the mind in the morning and calm the nerves at night. Furthermore, this herbal combination extraction greatly reduces the dosage of traditional Chinese medicine used, increasing the release efficiency and efficacy of the spray / ointment. The raw materials are inexpensive and readily available, the resulting herbal combination extract is easy to preserve, the production process is simple, the effects are obvious, the operation is simple and convenient, environmentally friendly and hygienic, easy to clean, and pollution-free.

Description

[0001] This case is a divisional application of the original application. The original patent number is 2022110874247; the original patent title is: Preparation and application of a Chinese herbal aromatherapy spray and ointment extract with multiple activities; the original application date is September 7, 2022. Technical Field

[0002] This invention belongs to the field of traditional Chinese medicine, specifically relating to the preparation and application of a traditional Chinese medicine aromatherapy spray and ointment extract with multiple activities. Background Technology

[0003] In today's fast-paced society, people experience increased stress. Prolonged sleep deprivation and other factors lead to weakened immunity, general weakness, difficulty falling asleep, and forgetfulness. Poor sleep quality also results in headaches, heaviness upon waking, and difficulty fully alert. Furthermore, mosquito bites at night significantly disrupt sleep for those with poor sleep quality. While many modern mosquito control products exist, most rely on chemical agents. Long-term use of these agents can lead to mosquito resistance, making them difficult to eradicate and increasing the risk of various infectious diseases, posing a significant threat to people's physical and mental health.

[0004] Aromatherapy, a tradition passed down from ancient my country, is known for its benefits such as accelerating cell metabolism, strengthening the immune system, eliminating toxins from cells, purifying the air, repelling insects, and killing bacteria. Often accompanied by soothing music, it promotes relaxation, relieves fatigue, stabilizes the endocrine system, and naturally leads to a refreshed mind and increased vitality. Based on this, this invention closely integrates ancient aromatherapy culture with modern Traditional Chinese Medicine (TCM) theory. Using a lamp as a medium, it incorporates TCM aromatherapy elements to create a TCM aromatherapy spray / stick with the main functions of opening the mind and refreshing the spirit in the morning, and calming the nerves, aiding sleep, and enhancing intelligence and awareness in the evening.

[0005] Based on the purpose of epidemic prevention, the formula of traditional Chinese medicine aromatherapy has been improved by adding herbs such as Eupatorium fortunei, Ligusticum striatum, and Pogostemon cablin, which have the activity of eliminating plague and warding off evil, as well as herbs such as Scutellaria baicalensis and Lonicera japonica, which have antibacterial activity. Using a certain preparation process, this aromatherapy paste has become a traditional Chinese medicine aromatherapy spray or aromatherapy paste that integrates multiple pharmacological activities such as eliminating plague and warding off evil, opening the mind and refreshing the spirit, calming the nerves, repelling insects and killing mosquitoes, purifying the air and antibacterial.

[0006] This invention provides a traditional Chinese medicine composition with multiple pharmacological activities, including dispelling plague and foulness, clearing the mind and refreshing the spirit, calming the nerves, repelling insects and killing mosquitoes, purifying the air, and antibacterial properties. The composition includes peppermint, calamus, clove, patchouli, jasmine, atractylodes, artemisia, eupatorium, ligusticum, lavender, olive leaf, rose, mugwort, nutmeg, sophora japonica, honeysuckle, scutellaria, peppermint, and cinnamon. The composition exhibits significant pharmacological activity and enhanced efficacy, helping to reduce the dosage of other medications. The raw materials are inexpensive and readily available, the resulting extract is easy to preserve, the production process is simple, the effects are obvious, and the operation is simple, convenient, environmentally friendly, hygienic, easy to clean, and pollution-free. Summary of the Invention

[0007] This invention addresses the shortcomings of current aromatherapy sprays and solid aromatherapy technology in the context of high social pressure. It provides a Chinese herbal aromatherapy spray extract and a Chinese herbal solid aromatherapy paste that are simple to produce, have obvious effects, are easy to operate, are environmentally friendly and hygienic, easy to clean, and have a pleasant fragrance.

[0008] The present invention solves the technical problem through the following solution:

[0009] Following the principles of ancient Chinese aromatherapy culture and supported by traditional Chinese medicine theory, this product addresses prominent issues in contemporary society such as high stress levels, insomnia, difficulty waking up, severe mosquito bites, and frequent influenza outbreaks. It utilizes a formula of Chinese herbs including: peppermint, calamus, clove, patchouli, jasmine, atractylodes, artemisia, eupatorium, ligusticum, lavender, olive leaf, rose, mugwort, nutmeg, sophora japonica, honeysuckle, scutellaria, peppermint, and cinnamon. These herbs are combined with modern pharmacological studies and formulated into appropriate herbal aromatherapy sprays and solid aromatherapy pastes.

[0010] In a first aspect, the present invention provides a traditional Chinese medicine composition with multiple activities, wherein the traditional Chinese medicine composition is selected from the following components in parts by weight: 3-5 parts of peppermint, 1-2 parts of acorus tatarinowii, 0.5-1 part of clove, 2-3 parts of patchouli, 3-5 parts of jasmine, 1-2 parts of atractylodes lancea, 1-2 parts of artemisia annua, 1-2 parts of eupatorium fortunei, 1-2 parts of ligusticum striatum, 2-3 parts of lavender, 3-5 parts of olive leaf, 1-2 parts of rose, 1-2 parts of mugwort, 0.5-1 part of nutmeg, 2-3 parts of sophora japonica, 1-2 parts of honeysuckle, 1-2 parts of scutellaria baicalensis, and 0.5-1 part of cinnamon.

[0011] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 5 parts peppermint, 2 parts acorus tatarinowii, 0.5 parts clove, 2 parts patchouli, 3 parts jasmine, 1 part atractylodes lancea, 1 part artemisia annua, 1 part eupatorium fortunei, and 1 part ligusticum striatum.

[0012] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 3 parts lavender, 3 parts olive leaves, 1 part rose, 1 part mugwort, 1 part white wormwood, 0.5 parts cinnamon, 0.5 parts nutmeg, 1 part agastache rugosa, and 1 part ligusticum striatum.

[0013] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 3 parts peppermint, 3 parts patchouli, 3 parts lavender, 2 parts mugwort, 3 parts sophora japonica, 2 parts honeysuckle, 2 parts scutellaria baicalensis, and 0.5 parts cinnamon.

[0014] Secondly, the present invention provides a traditional Chinese medicine composition extract with multiple activities. The preparation method of the traditional Chinese medicine composition extract is as follows: the traditional Chinese medicine composition of each component mentioned in the first aspect is pulverized, 70% ethanol solution is added and refluxed twice, the extracts are combined, filtered, and the filtrate is concentrated under reduced pressure to obtain the traditional Chinese medicine composition extract.

[0015] Preferably, the method includes the following steps:

[0016] (1) Take the Chinese herbal medicine composition and pulverize it at high speed, pass it through a 60-mesh sieve, and collect the sieved powder;

[0017] (2) Take the powder described in step (1), add 6-8 times the amount of 70% ethanol solution, heat under low heat and reflux for 6 hours to extract the extract; filter and collect the filtrate;

[0018] (3) Repeat step (2) with the filter residue obtained in step (2);

[0019] (4) Combine the filtrates obtained in steps (2) and (3), and concentrate them under vacuum to recover ethanol, thereby obtaining the extract of the traditional Chinese medicine composition.

[0020] Thirdly, the present invention provides the application of the traditional Chinese medicine composition described in the first aspect and the extract of the traditional Chinese medicine composition described in the second aspect in the preparation of products for eliminating plague and warding off filth, and / or opening the orifices and refreshing the mind, and / or calming the nerves, and / or repelling insects and killing mosquitoes, and / or purifying the air, and / or antibacterial products.

[0021] Preferably, the product includes applications in traditional Chinese medicine aromatherapy pastes, sprays, moisturizing soaps, and hand sanitizers.

[0022] Fourthly, the present invention provides a traditional Chinese medicine aromatherapy paste, the preparation method of which is as follows: an adhesive, excipient, buffer and neutralizer are placed in a mortar and repeatedly ground until they are mixed evenly and the particle size is uniform; pure water at 50-70℃ is added, and the mixture is placed in a water bath at 65℃ and heated for at least 30 minutes while stirring continuously until the solution in the beaker becomes gelatinous white and nearly transparent; then the extract of the traditional Chinese medicine composition, preservative and volatile oil of traditional Chinese medicine described in the second aspect are added, and the mixture is stirred repeatedly and mixed evenly, and left at room temperature until it becomes a solid paste.

[0023] Fifthly, the present invention provides a traditional Chinese medicine aromatherapy spray, wherein the preparation method of the traditional Chinese medicine aromatherapy spray is as follows: the extract of the traditional Chinese medicine composition described in the second aspect is added to a spray emission device to obtain the traditional Chinese medicine aromatherapy spray.

[0024] The beneficial effects of the present invention are: (1) The Chinese medicine composition described in the present invention is safe and non-toxic, with no obvious contraindications, and is suitable for use by all people; (2) It is safer and more effective than the chemical reagent mosquito-killing products currently on the market; (3) It is especially suitable for sub-healthy people who are under too much pressure in life, have difficulty falling asleep at night and cannot wake up in the morning; (4) The addition of antiviral Chinese medicine makes it more suitable for the prevention and control of the current epidemic; (5) Both the Chinese medicine and the matrix are green and environmentally friendly, and the preparation process is simple; (6) The prepared Chinese medicine combination extract spray and ointment have good stability, are not easy to decompose, and can be stored for a long time; (7) The prepared Chinese medicine combination extract spray and ointment can be divided into multiple functions such as purifying the air, killing mosquitoes and insects, fighting the epidemic, clearing the head and eyes, and calming and hypnotizing, and can be used according to needs; (8) The formula of this product is reasonable, the aroma is mild and mellow, the acceptance range is wide, and it meets the universal needs of the public. Attached Figure Description

[0025] Figure 1 The prepared traditional Chinese medicine aromatherapy spray and its implementation method are shown;

[0026] Figure 2 The prepared traditional Chinese medicine aromatherapy solid ointment and its implementation method are shown;

[0027] Figure 3 Results of effective head-clearing and detoxifying effects of formulas F1 and F2 aromatherapy sprays. * This indicates that P < 0.05. ** This indicates that P < 0.01;

[0028] Figure 4 The sedative-hypnotic efficacy of aromatherapy sprays in formulations F1 and F3, compared to group ZH, was [not specified]. * This indicates that P < 0.05. *** This indicates that P < 0.001;

[0029] Figure 5 The air-freshening effectiveness of aromatherapy sprays in formulations F1, F2, and F3 compared to group XJ was as follows. * This indicates that P < 0.05;

[0030] Figure 6 Results of mosquito and insect repellent efficacy of formulations F1, F2, and F3 aromatherapy sprays (Note: Compared with group CH) * This indicates that P < 0.05. ** This indicates that P < 0.01;

[0031] Figure 7Results of the efficacy of aromatherapy ointments F1 and F2 in clearing the head and eyes: Note: Compared with group BM, ** This indicates that P < 0.01. *** This indicates that P < 0.001;

[0032] Figure 8 The sedative and hypnotic efficacy of aromatherapy ointments in formulations F1 and F3 was compared with that in group BM. ** This indicates that P < 0.01. *** This indicates that P < 0.001;

[0033] Figure 9 The air-freshening efficacy of aromatherapy balms in formulations F1, F2, and F3 was compared to that in group XJ. * This indicates that P < 0.05. ** This indicates that P < 0.01. *** This indicates that P < 0.001;

[0034] Figure 10 The mosquito repellent and insecticidal efficacy of formulations F1, F2, and F3 aromatherapy ointments, compared to group CH, was [not specified]. ** This indicates that P < 0.01. *** This means P < 0.001. Detailed Implementation

[0035] To provide a detailed description of the technical solutions in this invention, the inventors have provided an explanation in conjunction with some embodiments. The inventors have the right to claim patent protection for similar traditional Chinese medicine aromatherapy sprays and solid ointments. It is worth noting that any technical solutions derived by those skilled in the art based on the concepts of this invention through logical analysis, deduction, and experimentation within the existing technology are all within the scope of protection claimed by this invention.

[0036] Example 1: Traditional Chinese Medicine Formula and Efficacy Evaluation

[0037] 1. The traditional Chinese medicine formulas described in this embodiment are as follows:

[0038] Traditional Chinese Medicine Formula 1 (F1): 3 parts peppermint, 2 parts acorus tatarinowii, 0.5 parts clove, 2 parts patchouli, 3 parts jasmine, 1 part atractylodes lancea, 1 part artemisia annua, 1 part eupatorium fortunei, 1 part ligusticum striatum, 3 parts lavender, 3 parts olive leaf, 1 part rose, 1 part mugwort, 0.5 parts nutmeg, 3 parts sophora japonica, 2 parts honeysuckle, 2 parts scutellaria baicalensis, 0.5 parts cinnamon;

[0039] Chinese herbal formula 2 (F2): 3 parts peppermint, 2 parts acorus tatarinowii, 0.5 parts clove, 2 parts patchouli, 3 parts jasmine, 1 part atractylodes lancea, 1 part artemisia annua, 1 part eupatorium fortunei, and 1 part ligusticum striatum.

[0040] Chinese herbal formula 3 (F3): 3 parts lavender, 3 parts olive leaves, 1 part rose, 1 part mugwort, 1 part white wormwood, 0.5 parts cinnamon, 0.5 parts nutmeg, 1 part agastache, 1 part ligusticum.

[0041] Chinese herbal formula 4 (F4): 3 parts peppermint, 3 parts patchouli, 3 parts lavender, 2 parts mugwort, 3 parts sophora japonica, 2 parts honeysuckle, 2 parts scutellaria baicalensis, and 0.5 parts cinnamon.

[0042] Five portions of each of the four traditional Chinese medicine formulas 1-4 were taken and prepared into traditional Chinese medicine aromatherapy spray and traditional Chinese medicine aromatherapy ointment respectively. The preparation of the traditional Chinese medicine aromatherapy spray is as follows: (1) Preparation of traditional Chinese medicine composition extract: ① Take each component of the traditional Chinese medicine composition and pulverize it at high speed, pass it through a 60-mesh sieve, and collect the sieved powder; ② Add 6-8 times the amount of 70% ethanol solution to the powder, heat it under low heat and reflux for 6 hours; filter and collect the extract; ③ Take the filter residue and repeat step ②; ④ Collect the extract twice, concentrate it under vacuum and reduce pressure to recover the ethanol, until the extract has no obvious ethanol smell, and then obtain the traditional Chinese medicine composition extract. Sterilize it at high temperature, seal it and place it at room temperature, and store it at 4℃ for later use; (2) Preparation of traditional Chinese medicine aromatherapy spray: Take 1 mL of the above traditional Chinese medicine combination extract, dilute it with pure water to 10 mL, and then use a spray launcher to obtain traditional Chinese medicine aromatherapy spray, which can stably and persistently release for 2-5 hours. The preparation process is as follows. Figure 1 As shown.

[0043] The preparation of the traditional Chinese medicine aromatherapy ointment is as follows: (1) Preparation of the extract of the traditional Chinese medicine composition: Same as above (1); (2) Preparation of the traditional Chinese medicine aromatherapy ointment: Add the adhesive and excipient in a beaker at a ratio of 11:7 (that is, when 11 parts of adhesive are needed by mass, 7 parts of excipient are needed), then add 2 parts of buffer and 0.8 parts of neutralizer, place in a mortar and grind repeatedly until the mixture is uniform and the particle size is uniform, then add 50mL of pure water (keep it between 50-70℃). Place the mixture in a 65℃ water bath and heat for at least 30 minutes, stirring constantly, until the solution in the beaker becomes gelatinous white and nearly transparent. Then, add 10mL of the herbal extract, 0.2 parts of preservative, and one or more herbal volatile oils, stirring repeatedly until well mixed. Let it stand at room temperature until the solution in the beaker becomes a solid paste with a uniform color, similar to the color of the medicinal liquid. This yields a solid herbal aromatherapy paste. Allow it to cool naturally at room temperature, then seal it in a vacuum-sealed bag and store it at 4℃. When using it in conjunction with a multi-functional aromatherapy lamp, it can stably last for 0.5-5 hours. Figure 2 As shown.

[0044] 2. In vitro experiments

[0045] The aromatherapy spray and herbal aromatherapy paste of herbal formula 1 (F1) were tested for their effects of opening the mind and stimulating consciousness, calming the nerves, repelling insects and killing mosquitoes, purifying the air, and antibacterial activity. The aromatherapy spray and herbal aromatherapy paste of herbal formula 2 (F2) were tested for their effects of clearing the head and eyes, purifying the air, repelling mosquitoes and killing insects, and antibacterial activity. The aromatherapy spray and herbal aromatherapy paste of herbal formula 3 (F3) were tested for their effects of calming and hypnotizing, purifying the air, repelling mosquitoes and killing insects, and antibacterial activity. The aromatherapy spray and herbal aromatherapy paste of herbal formula 4 (F4) were tested for antibacterial activity. Five samples of each formula were selected in parallel for the experiment (numbered: F1-1~F1-5; F2-1~F2-5; F3-1~F3-5; F4-1~F4-5).

[0046] The following tests were conducted: A 4ml sample of Baimiss Energizing Energy Bar was used as a comparison for the use of a commercially available product; five parallel samples (numbered BM-1 to BM-5) were selected for each test; a 200ml sample of Liushen Floral Water was used as a comparison for the use of a commercially available product; a 70g sample of Xinjushuang Solid Air Freshener was used as a comparison for the use of a commercially available product; and a 200ml sample of Liushen Floral Water was used as a comparison for the use of a commercially available product. Five parallel samples were selected for each test.

[0047] The dosage and administration are as follows: twice daily (once in the morning and once in the evening), for a course of 7 days. The number of subjects in each study is 50. Effectiveness is determined by significant reduction in the corresponding symptoms of the subjects or by subjective evaluation of effectiveness. Efficacy rate (%) = (number of subjects / total number of subjects) × 100%.

[0048] Among them, as shown in Table 1 and Figure 3 As shown, the total effective rate of F1 aromatherapy spray in clearing the head and saliva was 84.91±11.97, while the total effective rate of the control group BM was 74.34±11.92, indicating that the effective rate of F1 aromatherapy spray in clearing the head and saliva was significantly higher than that of the control group (P<0.01). The total effective rate of F2 aromatherapy spray in clearing the head and saliva was 82.74±12.03, while the total effective rate of the control group BM was 74.34±11.92 (P<0.05), indicating that the effective rate of F2 aromatherapy spray in clearing the head and saliva was higher than that of the control group. This shows that both F1 and F2 aromatherapy sprays have significant activity in clearing the head and saliva, and F1 is superior to F2. Among them, compared with the BM group, * This indicates that P < 0.05. ** This indicates that P < 0.01.

[0049] Table 1. Effectiveness of Aromatherapy Sprays Formulas F1 and F2 in Clearing Head Muscles

[0050]

[0051]

[0052] As shown in Table 2 and Figure 4 As shown, the total effective rate of sedation and hypnosis of Formula F1 aromatherapy spray was 85.60±8.78%, while the total effective rate of the control group ZH was 75.94±10.08%, indicating that the effective rate of sedation and hypnosis of Formula F1 was higher than that of the control group; the total effective rate of sedation and hypnosis of Formula F3 aromatherapy spray was 81.33±10.29%, while the total effective rate of sedation and hypnosis of the control group ZH was 75.94±10.08%, indicating that the effective rate of sedation and hypnosis of Formula F3 aromatherapy spray was higher than that of the control group. This indicates that both Formula F1 and Formula F3 aromatherapy sprays have significant activity in clearing the head and eyes, and Formula F1 is superior to F3. In particular, compared with Group ZH, * This indicates that P < 0.05. *** This means P < 0.001.

[0053] Table 2. Results of the sedative and hypnotic efficacy of aromatherapy sprays formulated F1 and F3.

[0054]

[0055] Combination Figure 5 The air freshening efficacy of Formula F1 aromatherapy spray is shown in Table 3, with a total effective rate of 90.51±7.35, while the total effective rate of the control group XJ was 85.83±8.90. Formula F1 aromatherapy spray is significantly better than the control group (P<0.05). The air freshening efficacy of Formula F2 aromatherapy spray is shown in Table 4, with a total effective rate of 87.49±7.88. Formula F2 aromatherapy spray is better than the control group, but the difference is not statistically significant, indicating that the effects of F2 aromatherapy spray and control XJ are comparable. The air freshening efficacy of Formula F3 aromatherapy spray is shown in Table 5, with a total effective rate of 89.71±6.75. Formula F3 aromatherapy spray is better than the control group, but the difference is not statistically significant, indicating that the effects of F2 aromatherapy spray and control XJ are comparable. The experimental results showed that all three aromatherapy sprays (formulas F1, F2, and F3) had an air-freshening effect, with F1 being the most effective. F2 and F3 were roughly equivalent to the control group on the market. However, compared to group XJ... * This indicates that P < 0.05.

[0056] Table 3 shows the air freshening effectiveness of Formula F1 aromatherapy spray.

[0057]

[0058]

[0059] Table 4 shows the air freshening effectiveness of Formula F2 aromatherapy spray.

[0060]

[0061] Table 5 shows the air freshening effectiveness of Formula F3 aromatherapy spray.

[0062]

[0063] Combination Figure 6 The efficacy results of the F1 aromatherapy spray for mosquito and insect control are shown in Table 6. The total efficacy rate was 78.17±12.25, while the total efficacy rate of the control group CH was 74.80±12.08. The efficacy of the F1 aromatherapy spray for mosquito and insect control was significantly better than that of the control group (P<0.05). The efficacy results of the F2 aromatherapy spray for mosquito and insect control are shown in Table 7. The total efficacy rate was 83.20±8.40. The efficacy of the F2 aromatherapy spray for mosquito and insect control was significantly better than that of the control group (P<0.01). The efficacy results of the F3 aromatherapy spray for mosquito and insect control are shown in Table 8. The total efficacy rate was 79.83±10.31. The F3 aromatherapy spray for mosquito and insect control was better than that of the control group, but the difference was not statistically significant, indicating that the F3 aromatherapy spray for mosquito and insect control was comparable to that of the control CH. The experimental results showed that formulations F1, F2, and F3 of the aromatherapy spray all had significant mosquito-repellent and insecticidal effects. Formulations F1 and F2 showed better mosquito and insect repellent effects, while the effect of formulation F3 was basically the same as the control. Note: Compared with group CH, * This indicates that P < 0.05. ** This indicates that P < 0.01.

[0064] Table 6 shows the effectiveness of formulation F1 aromatherapy spray in repelling mosquitoes and killing insects.

[0065]

[0066] Table 7 shows the effectiveness of F2 aromatherapy spray in repelling mosquitoes and killing insects.

[0067]

[0068] Table 8 shows the effectiveness of formulation F3 aromatherapy spray in repelling mosquitoes and killing insects.

[0069]

[0070] As shown in Table 9 and Figure 7As shown, the total effective rate of the F1 aromatherapy balm for clearing the head and saliva was 84.97±9.04, while the total effective rate of the control group BM was 76.80±7.43. The effective rate of the F1 aromatherapy balm for clearing the head and saliva was significantly better than that of the control group (P<0.01). The total effective rate of the F2 aromatherapy balm for clearing the head and saliva was 86.51±7.49, and the effective rate of the F2 aromatherapy spray for clearing the head and saliva was significantly better than that of the control group (P<0.001). This indicates that both the F1 and F2 aromatherapy balms have significant activity in clearing the head and saliva. Among them, compared with the BM group, ** This indicates that P < 0.01. *** This means P < 0.001.

[0071] Table 9 shows the effectiveness of formulas F1 and F2 aromatherapy ointments in clearing the head and eyes.

[0072]

[0073] As shown in Table 10 and Figure 8 As shown, the total effective rate of sedation and hypnosis of Formula F1 aromatherapy ointment was 88.79±6.21%, while the total effective rate of the control group ZH was 80.46±9.80%. The effective rate of sedation and hypnosis of Formula F1 aromatherapy ointment was significantly higher than that of the control group (P<0.001). The total effective rate of sedation and hypnosis of Formula F3 aromatherapy ointment was 89.71±5.72%, while the total effective rate of the control group ZH was 80.46±9.80. The effective rate of sedation and hypnosis of Formula F3 aromatherapy ointment was significantly higher than that of the control group (P<0.001). This indicates that both Formula F1 and Formula F3 aromatherapy ointments have significant activity in clearing the head and eyes. Among them, compared with Group BM, *** This indicates that P < 0.01.

[0074] Table 10 shows the sedative and hypnotic efficacy of aromatherapy ointments F1 and F3 in formulations 10.

[0075]

[0076] Combination Figure 9The air-freshening efficacy of formula F1 aromatherapy balm is shown in Table 11, with a total effective rate of 90.17±5.40, while the total effective rate of the control group XJ was 83.94±7.89. Formula F1 aromatherapy balm showed a significantly better air-freshening effect than the control group (P<0.001). The air-freshening efficacy of formula F2 aromatherapy balm is shown in Table 12, with a total effective rate of 89.31±4.87. Formula F2 aromatherapy balm showed a significantly better air-freshening effect than the control group (P<0.01). The air-freshening efficacy of formula F3 aromatherapy balm is shown in Table 13, with a total effective rate of 89.14±5.08. Formula F3 aromatherapy balm showed a better air-freshening effect than the control group (P<0.05). These experimental results show that formulas F1, F2, and F3 aromatherapy balms all have significant air-freshening effects. Compared with group XJ, * This indicates that P < 0.05. ** This indicates that P < 0.01. *** This means P < 0.001.

[0077] Table 11. Air-freshening efficacy of Formula F1 aromatherapy balm.

[0078]

[0079] Table 12 shows the air-freshening effectiveness of Formula F2 aromatherapy balm.

[0080]

[0081] Table 13 shows the air-freshening efficacy of Formula F3 aromatherapy balm.

[0082]

[0083] Combination Figure 10 The efficacy results of the F1 aromatherapy ointment for mosquito and insect control are shown in Table 14, with a total efficacy of 78.17±12.25, while the total efficacy of the control group CH was 74.80±12.07. The efficacy of the F1 aromatherapy ointment for mosquito and insect control was significantly better than that of the control group (P<0.001). The efficacy results of the F2 aromatherapy ointment for mosquito and insect control are shown in Table 15, with a total efficacy of 85.49±9.12. The efficacy of the F2 aromatherapy ointment for mosquito and insect control was significantly better than that of the control group (P<0.01). The efficacy results of the F3 aromatherapy ointment for mosquito and insect control are shown in Table 16, with a total efficacy of 86.00±8.64. The efficacy of the F3 aromatherapy ointment for mosquito and insect control was stronger than that of the control group, but the difference was not statistically significant, indicating that the efficacy of the F3 aromatherapy ointment for mosquito and insect control was comparable to that of the control CH. The experimental results showed that formulations F1 and F2 of the aromatherapy paste had significant mosquito-repellent and insecticidal effects, while formulation F3 of the aromatherapy paste had the same mosquito-repellent and insecticidal effects as the control. Among these, compared with group CH, ** This indicates that P < 0.01. ***This means P < 0.001.

[0084] Table 14 shows the effectiveness of Formula F1 aromatherapy ointment in repelling mosquitoes and killing insects.

[0085]

[0086] Table 15 shows the effectiveness of Formula F2 aromatherapy ointment in repelling mosquitoes and killing insects.

[0087]

[0088] Table 16 shows the effectiveness of Formula F3 aromatherapy ointment in repelling mosquitoes and killing insects.

[0089]

[0090]

[0091] 3. Animal experiments

[0092] Preparation of extracts from traditional Chinese medicine formulas:

[0093] Take each component of the Chinese herbal medicines F1-F4, add 10 times the amount of purified water, and decoct for 1 hour. Decoction twice, collect the extracts from both decoctions, and reflux extract over low heat for 6 hours; extract twice. Collect the extracts from both decoctions, concentrate them at low temperature to a crude drug content of 0.5 g / mL, and store at 4°C for later use.

[0094] (1) Head-Clearing Experiment: The evaluation indicators of this experiment are mainly norepinephrine (NA) and dopamine (Da) in brain tissue. Norepinephrine is a neurotransmitter, a chemical substance responsible for transmitting nerve impulses between neurons. It can be found in the central and sympathetic nervous systems. This substance is also secreted by the adrenal glands and has the effect of a stress hormone. It can excite the heart and help people wake up quickly and be full of energy. Dopamine is a brain endocrine substance and the most abundant catecholamine neurotransmitter in the brain. It can regulate a variety of physiological functions of the central nervous system. It is related to human emotions and feelings and can transmit information of excitement and happiness. Therefore, NA and Da are used as indicators to evaluate the effect of the head-clearing formula in this experiment.

[0095] Experimental Methods: Ninety SD rats (180±20g) were randomly divided into nine groups: blank group, model group, norepinephrine group, dopamine group, norepinephrine + dopamine group, F1 drug administration group, F2 drug administration group, F3 drug administration group, and F4 drug administration group. Except for the blank group, hypoxic-ischemic encephalopathy models were established in the other groups. The specific method was as follows: After anesthetizing the rats, the skin was incised in the midline of the neck, the common carotid artery was separated, and double ligation was performed using 5-0 non-invasive sutures. The blood vessel was cut in the middle of the ligature, disinfected, and sutured. Two hours after surgery, the rats were placed in a hypoxic chamber, and a mixture of 92% nitrogen and 8% oxygen was introduced into the chamber for continuous hypoxia for 2 hours. After successful model establishment, each group was administered medication by gavage for 7 days at a dose of 20mL / kg. After the administration period, the levels of NA and Da in the rat brain tissue fluid were detected using an ELISA kit. The results are shown in Table 17. Compared with the blank group, ### This indicates that P < 0.001; compared with the model group, * This indicates that P < 0.05. ** This indicates that P < 0.01. *** This means P < 0.001.

[0096] As shown in Table 17, compared with the control group, the levels of NA and Da in the model group were significantly decreased (P < 0.001). After treatment, the levels of NA and Da in the F1-F4 treatment groups were significantly increased (P < 0.05 or P < 0.01). This indicates that all the formulations promoted the increase of NA and Da in brain tissue, and F1-F4 all had hormone-like effects similar to NA and Da. These results suggest that F1-F4 all have a clearing and refreshing effect on the head, and after use, they can have an excitatory effect, helping people to quickly regain consciousness and vitality.

[0097] Table 17 Expression levels of NA and Da in the brain tissue fluid of rats in each group (n=8, )

[0098]

[0099]

[0100] (2) Sedative-Hypnotic Experiment: The main evaluation indicators for this experiment were serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) in brain tissue. Serotonin, also known as serotonin, is a widely distributed biological indoleamine. It is both a neurotransmitter and a vasoactive substance. The serotonin neurotransmitter system in the brain is relatively concentrated, with its neurons mainly located in the raphe nuclei in the lower brainstem near the midline. Numerous studies have shown that 5-HT, as an important monoamine neurotransmitter in the brain, plays an irreplaceable role in improving insomnia symptoms. 5-HT has a significant effect on promoting both REM and SEM sleep. The insomnia rat model was created by micro-injecting p-chlorophenylalanine, which reduces 5-HT synthesis, into the dorsal raphe nucleus of rats, thus inducing insomnia symptoms. Therefore, 5-HT was used as an effective indicator for evaluating sedative-hypnotic effects.

[0101] Experimental Methods: Establishment of an animal model of insomnia: Seventy Kunming mice (16±2g) were acclimatized for 1 week and then randomly divided into 7 groups according to body weight: blank control group, model group, diazepam 2.6mg / kg group, F1 treatment group, F2 treatment group, F3 treatment group, and F4 treatment group. Except for the blank control group, which received an equal volume of physiological saline intraperitoneally, the other groups were given a 30mg / mL chlorophenylalanine (PCPA) saline solution. The solution was thoroughly shaken before each injection, and the injection was administered once daily at a volume of 10mL / kg for 2 days. Compared with the blank control group, mice exhibiting restlessness, continuous activity, loss of diurnal rhythm, decreased appetite, and significant weight loss were considered to have successfully established the insomnia model. After successful modeling, the mice were administered the corresponding drugs via gavage at a dose of 0.4 mL / 20 g / day for 6 consecutive days. Thirty minutes after the last gavage, each group of mice was given an intraperitoneal injection of 30 mg / kg sodium pentobarbital. The number of mice falling asleep within 20 minutes was observed, and the results are shown in Table 18. The time of disappearance and reappearance of the righting reflex were also recorded for each group of mice. The disappearance of the righting reflex for more than 30 seconds was considered sleep initiation, and the sleep time from sleep onset to the reappearance of the righting reflex was defined as sleep duration. Finally, the sleep latency and sleep duration for each group were calculated, and the results are shown in Table 19. After the experiment, cerebrospinal fluid was collected and 5-HT levels were detected using an ELISA kit, and the results are shown in Table 20. Compared with the blank group, #### This indicates that P < 0.0001; compared with the model group, * This indicates that P < 0.05. ** This indicates that P < 0.01. *** This means P < 0.001.

[0102] Table 18 shows the effects of each traditional Chinese medicine formula on the number of times the mice in the PCPA insomnia model fall asleep. It is evident that F1-F4 all significantly increased the sleep rate of the PCPA insomnia model mice, but none were as effective as diazepam. F1 showed the best effect, followed by F3, then F4, and F2 showed the worst. Table 19 shows the effects of each traditional Chinese medicine formula on the sleep of the PCPA insomnia model mice. It is evident that, compared with the control group, the sleep latency in the model group was significantly prolonged (P < 0.05). 0001), sleep duration was significantly reduced (P<0.0001), sleep latency was significantly shortened (P<0.01 or (P<0.001) and sleep duration was significantly prolonged (P<0.01 or (P<0.001)) in each treatment group. The traditional Chinese medicine formulas F1-F4 all had significant effects on improving sleep, effectively reducing sleep latency and increasing sleep duration. Among them, F1 had the most significant effect, followed by F3, then F4, and F2 had the worst effect.

[0103] Table 18. Effects of various traditional Chinese medicine formulations on the number of mice falling asleep in the PCPA insomnia model (n=10)

[0104] Group Number of babies falling asleep (babies) Sleep onset rate (%) Blank group 0 0％ Model group 0 0％ Diazepam 2.6 mg / kg group 7 70％ F1 treatment group 6 60％ F2 drug administration group 2 20％ F3 treatment group 5 50％ F4 treatment group 4 40％

[0105] Table 19 Effects of various traditional Chinese medicine formulations on sleep in PCPA insomnia model mice (n=10, )

[0106] Group Sleep latency (min) Sleep duration (min) Blank group 3.33±1.18 72.50±5.63 Model group <![CDATA[9.36±1.29 #### ]]> <![CDATA[16.60±2.41 #### ]]> Diazepam 2.6 mg / kg group <![CDATA[1.95±0.74 *** ]]> <![CDATA[80.40±5.95 *** ]]> F1 treatment group <![CDATA[3.07±0.70 *** ]]> <![CDATA[65.00±5.98 *** ]]> F2 drug administration group <![CDATA[6.91±0.84* * ]]> <![CDATA[35.44±5.78 *** ]]> F3 treatment group <![CDATA[3.20±0.58 *** ]]> <![CDATA[60.60±6.92 *** ]]> F4 treatment group <![CDATA[7.46±0.50 ** ]]> <![CDATA[50.00±7.31 *** ]]>

[0107] Table 20. Expression levels of 5-HT in the brain tissue fluid of rats in each group (n=8, )

[0108] Group 5-HT (ng / g) Blank group 246.88±25.51 Model group <![CDATA[161.38±17.66 ### ]]> Diazepam 2.6 mg / kg group <![CDATA[282.63±11.54 *** ]]> F1 treatment group <![CDATA[228.75±12.70 *** ]]> F2 drug administration group 187.00±11.87 F3 treatment group <![CDATA[251.50±16.97 *** ]]> F4 treatment group <![CDATA[173.88±8.87 * ]]>

[0109] (3) Antibacterial test:

[0110] Example: Preparation of extract of traditional Chinese medicine composition: Mix the components of traditional Chinese medicine F1-F4, pulverize at high speed, pass through a 60-mesh sieve, and collect the sieved powder; add 6-8 times the amount of 70% ethanol solution, and gently heat under reflux for 6 hours; extract twice. Collect the two extracts, concentrate under vacuum to recover ethanol, and continue until the extract has no obvious ethanol smell, thus obtaining the extract of traditional Chinese medicine composition. Sterilize at high temperature, seal and place at room temperature, then store at 4℃ for later use.

[0111] Comparative example:

[0112] Preparation of single-herb extracts: Weigh out 30g each of peppermint, acorus tatarinowii, clove, patchouli, jasmine, atractylodes lancea, artemisia annua, eupatorium fortunei, ligusticum striatum, lavender, olive leaf, rose, mugwort leaf, nutmeg, sophora japonica flower, honeysuckle, scutellaria baicalensis, and cinnamon; the preparation method is the same as that for the preparation of the herbal combination extract in the example.

[0113] Comparative composition 1 (Z1): 30g of Sophora japonica flower, 20g of Lonicera japonica flower, 20g of Scutellaria baicalensis flower, and 5g of Cinnamomum cassia bark; Comparative composition 2 (Z2): 30g of Mentha haplocalyx flower, 30g of Pogostemon cablin flower, 20g of Lavender flower, and 20g of Artemisia argyi leaf; Comparative composition 3 (Z3): 20g of Acorus tatarinowii flower, 5g of Syzygium aromaticum flower, 30g of Jasmine flower, 10g of Atractylodes lancea flower, 10g of Artemisia argyi flower, 10g of Eupatorium fortunei flower, and 10g of Ligusticum striatum flower; Comparative composition 4 (Z4): 30g of Olive leaf, 10g of Rose, 10g of Artemisia argyi leaf, and 5g of Nutmeg; The preparation method is the same as that for the preparation of the herbal composition extract in the examples.

[0114] Inhibition zone detection: The agar plate perforation method was used. 100 μL of the test bacteria was evenly spread onto nutrient agar. After 15 min, holes were evenly punched in the nutrient agar using a 6 mm diameter punch. The agar block was removed. 1 mL of the herbal extract was added to each well, ensuring the plate was full but not overflowing. Five wells were used per plate, with a well spacing >20 mm. Distilled water was placed in the center as a negative control, and a positive control was also included. The plates were incubated at 37℃ for 24 h. The diameter of the inhibition zone was measured, and the results were observed. The experiment was repeated three times. An inhibition zone diameter ≥20 mm was considered extremely sensitive, 15–20 mm as highly sensitive, 10–15 mm as moderately sensitive, and below 10 mm as lowly sensitive.

[0115] As shown in Table 21, the antibacterial results indicate that the extracts of various single Chinese herbal medicines, including Acorus tatarinowii, Atractylodes lancea, Artemisia argyi, Eupatorium fortunei, Lavender, Olive leaf, Rose, Artemisia argyi, Sophora japonica, Honeysuckle, Scutellaria baicalensis, and Cinnamon, exhibit certain antibacterial activity against Staphylococcus aureus. Among these, only Artemisia argyi and Scutellaria baicalensis show high antibacterial activity against Staphylococcus aureus. Artemisia argyi, Ligusticum striatum, Olive leaf, Rose, Artemisia argyi, Sophora japonica, and Scutellaria baicalensis also exhibit certain antibacterial activity against Streptococcus albus. Among these, only Rose and Artemisia argyi show high antibacterial activity against Streptococcus albus. Clove, Atractylodes lancea, Myristica fragrans, Sophora japonica, Scutellaria baicalensis, and Cinnamon also exhibit certain antibacterial activity against Escherichia coli. Among these, only Atractylodes lancea shows high antibacterial activity against Escherichia coli. As shown in Table 22, the antibacterial results of the herbal extracts in formulations F1-F4 indicate that all four formulations exhibit significant inhibitory activity against Staphylococcus aureus, Streptococcus albus, and Escherichia coli. Specifically, F1, F2, and F4 showed extremely sensitive antibacterial activity against Staphylococcus aureus, while F3 showed highly sensitive antibacterial activity. F1 and F4 showed extremely sensitive antibacterial activity against Streptococcus albus, while F2 and F3 showed highly sensitive antibacterial activity. All four formulations (F1, F2, F3, and F4) showed extremely sensitive antibacterial activity against Escherichia coli. These results demonstrate that F1 and F4 exhibit excellent antibacterial effects. The comparison of antibacterial results shown in Tables 21 and 22 reveals that the antibacterial effect of single herbs is significantly weaker, while the combined Chinese medicine formulas (F1, F2, F3, and F4) exhibit significant antibacterial activity. F1, which combines F2 and F3 with the main antibacterial active Chinese medicine of F4, possesses extremely strong antibacterial activity. The combinations of Chinese medicines F2 and F3 have considerable antibacterial activity, but all four formulas exhibit high antibacterial activity, and the overall antibacterial activity is significantly stronger than that of any single herb. Therefore, they can be used as a basis for the future development of related antibacterial products.

[0116] Table 21. Diameter of inhibition zones (mm) of different herbal extracts against the tested bacteria.

[0117]

[0118]

[0119] Table 22. Diameter of inhibition zones (mm) of different herbal extracts against the tested bacteria.

[0120]

[0121] As shown in Table 23, the extracts of the four comparative compositions all have a certain antibacterial effect on the detected bacteria, but the antibacterial effect is significantly lower than that of the traditional Chinese medicine composition described in this invention.

[0122] Table 23. Diameter of inhibition zones (mm) of the comparative composition extracts against the tested bacteria.

[0123]

[0124] Note: The description in this patent is merely an embodiment of the present invention and is not intended to limit the further development of the present invention. Any modifications, equivalent substitutions, and improvements based on the technology, spirit, and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition, characterized in that, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 3 parts lavender, 3 parts olive leaves, 1 part rose, 1 part mugwort, 1 part white wormwood, 0.5 parts cinnamon, 0.5 parts nutmeg, 1 part agastache rugosa, and 1 part ligusticum striatum. The preparation method of the traditional Chinese medicine composition is as follows: take the traditional Chinese medicine composition of each component, crush it, add 70% ethanol solution and reflux to extract twice, combine the extracts, filter, concentrate the filtrate under reduced pressure to obtain the extract of the traditional Chinese medicine composition.

2. The traditional Chinese medicine composition as described in claim 1, characterized in that, The preparation method includes the following steps: (1) Take the Chinese herbal medicine composition and pulverize it at high speed, pass it through a 60-mesh sieve, and collect the sieved powder; (2) Take the powder described in step (1), add 6-8 times the amount of 70% ethanol solution, heat under low heat and reflux for 6 h to extract the extract; filter and collect the filtrate; (3) Repeat step (2) with the filter residue obtained in step (2); (4) Combine the filtrates obtained in steps (2) and (3), and concentrate them under vacuum to recover ethanol, thereby obtaining the extract of the traditional Chinese medicine composition.

3. The use of the traditional Chinese medicine composition as described in claim 1 in the preparation of sedative and tranquilizing, and / or insect repellent and mosquito killer, and / or air purifier, and / or antibacterial products.

4. The application as described in claim 3, characterized in that, The products are Chinese herbal aromatherapy pastes, sprays, moisturizing soaps, and hand sanitizers.

5. A traditional Chinese medicine aromatherapy spray, characterized in that, The method for preparing the herbal aromatherapy spray is as follows: the herbal composition described in claim 1 is added to a spray emission device to obtain the herbal aromatherapy spray.

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