A method of cutting a scotch pine

Abstract

The application discloses a pinning method for pinus sylvestris var. mongolica, and belongs to the technical field of pinus sylvestris var. mongolica pinning, and the method comprises the following steps: (1) seedbed preparation: setting a pinning bed, laying river pebbles on the bottom of the pinning bed, and laying pinning matrix on the river pebbles; (2) cutting treatment: pruning tender branches to obtain cuttings, then soaking the tail end of the cuttings into a callus promoting agent, and obtaining treated cuttings after the soaking is completed; (3) pinning: vertically inserting the treated cuttings into the pinning bed after the treated cuttings are dried; and (4) pinning management: immediately covering the seedbed film with an arched plastic film after the pinning is completed, and keeping the relative humidity and temperature in the pinus sylvestris var. mongolica seedbed, so that the problem of low survival rate of pinus sylvestris var. mongolica pinning is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Pinus sylvestris var. Mongholica Litv. cutting, in particular to a cutting method of Pinus sylvestris var. Mongholica Litv. BACKGROUND

[0002] Pinus sylvestris var. Mongholica Litv. is a plant of Pinaceae and Pinus, mainly distributed in Daxing'anling forest region, and has the characteristics of strong adaptability, strong drought resistance and strong cold resistance. Pinus sylvestris var. Mongholica Litv. has developed root system, luxuriant branches and leaves, good fast-growing ability, strong resistance and wind resistance. At present, as one of the main afforestation tree species in the north, the artificial forest has various ecological benefits such as wind prevention and sand fixation, water conservation, agricultural yield increase, carbon fixation and oxygen release.

[0003] Cutting propagation refers to cutting a section of plant vegetative organ, using its regeneration ability to make it root and branch, and becoming a new plant. The cutting method can not only effectively alleviate the shortage of seedlings, but also improve the genetic quality of seedlings. However, since Pinus sylvestris var. Mongholica Litv. belongs to callus rooting, only a small amount of bark can root, and only after callus rooting is met can bark rooting be carried out, and the rooting time is long. Moreover, it is difficult to form callus at low temperature, which leads to high requirement of Pinus sylvestris var. Mongholica Litv. cutting on temperature, and the cutting of Pinus sylvestris var. Mongholica Litv. is difficult to be carried out in autumn. On the other hand, the cutting stock is usually selected from 2-3 year old mother trees with high rooting rate, and with the increase of the age of mother trees, the rooting ability of cutting stock gradually decreases. If mother trees with large age are selected, the survival rate of cutting is low, and the cost is increased, which also leads to less available cutting stock. This is because with the increase of the age of mother trees, the cells gradually age, the cell activity and metabolic capacity decrease, the callus of cutting stock is difficult to form, and the rooting is difficult. The low rooting rate leads to that the cutting stock cannot effectively absorb water and nutrients, the growth of cutting stock is limited, and it is difficult to survive. At present, the cutting stock is mainly soaked or dipped in naphthalene acetic acid solution, rooting powder solution and other methods to promote rooting and improve survival rate. However, for cutting stock with large age of mother trees, the survival rate is improved to a limited extent.

[0004] Therefore, the present application provides a cutting method of Pinus sylvestris var. Mongholica Litv., which reduces the requirement of cutting stock on the age of mother trees, promotes the rooting of Pinus sylvestris var. Mongholica Litv. cutting stock, and improves the survival rate of Pinus sylvestris var. Mongholica Litv. cutting. SUMMARY

[0005] In view of this, the purpose of the present application is to provide a cutting method of Pinus sylvestris var. Mongholica Litv., which reduces the requirement of cutting stock on the age of mother trees and improves the survival rate of cutting.

[0006] The present application solves the above technical problems by the following technical means:

[0007] (1) Seedbed preparation: Set up a cutting bed with a width of 1.2 m and a thickness of 0.5 m, lay river pebbles on the bottom layer of the cutting bed, and lay cutting substrate on top. Disinfect the seedbed with a 0.5wt% potassium permanganate solution 7-10 days before cutting;

[0008] (2) Cutting treatment: Select tender branches with terminal buds and no pests or diseases, trim them to 12-15 cm, then cut the trimmed tender branches from the bottom 4 cm or so to the base of the branch in a diagonal cutting manner, obtain the cuttings, then soak the ends of the cuttings in a callus-promoting agent, and obtain the treated cuttings after soaking is complete;

[0009] (3) Cutting: After the treated cuttings are dried, they are inserted vertically into the cutting bed, with a cutting depth of 1 / 3 of the length of the cuttings, and a cutting row spacing of 4 cm x 3 cm;

[0010] (4) Cutting management: After cutting is complete, immediately cover the seedbed with an arched plastic film, and maintain the relative humidity and temperature in the seedbed.

[0011] Further, the cutting substrate in step (1) is compounded from perlite and grass charcoal in a mass ratio of 2:1.

[0012] Further, the length of the cutting in step (2) is 2-3 cm above the cut.

[0013] Further, the soaking time of the cutting in step (2) is 25-35 min.

[0014] Further, the callus-promoting agent in step (2) comprises the following raw materials: oxalacetic acid, Gleditsia sinensis extract, pyrroloquinoline quinone, sodium lactate, danshensu, and methyl-β-cyclodextrin.

[0015] Further, the proportions of the raw materials of the callus-promoting agent are as follows:

[0016] The mass ratio of oxalacetic acid, Gleditsia sinensis extract, pyrroloquinoline quinone, sodium lactate, danshensu, and methyl-β-cyclodextrin is (1-1.2):(5-6):(0.6-0.8):(0.6-0.7):(0.5-0.6):(1.2-1.5).

[0017] Further, the preparation method of the callus-promoting agent is as follows:

[0018] A: Grind Gleditsia sinensis and add it to a 50wt% ethanol solution, ultrasonically extract it, and take the clear liquid to obtain Gleditsia sinensis extract;

[0019] B: the Danshensu is added into 50% ethanol solution, stirred uniformly to obtain Danshensu solution, then methyl-β-cyclodextrin is grinded into paste after adding water, the Danshensu solution is added, and grinded for 1-1.5h, and then dried at 40-50℃ for 1.5-2h to obtain the inclusion compound;

[0020] C: the inclusion compound is added into deionized water, stirred at 250-350r / min for 5-10min, then oxalacetic acid, pyrroloquinoline quinone and sodium lactate are added, and stirred until completely dissolved, then the Gleditsia sinensis extract is added, and mixed uniformly to obtain the callus promoting agent.

[0021] Further, in the callus promoting agent preparation method, the ratio of Gleditsia sinensis to ethanol solution in step A is 1:40, the ultrasonic extraction power of Gleditsia sinensis is 300-350W, the extraction temperature is 50-60℃, and the extraction time is 15-20min.

[0022] Further, the mother tree of the Pinus sylvestris var. mongolica cutting is 2-10 years old.

[0023] The Pinus sylvestris var. mongolica cutting is generally selected from a 2-3 year old mother tree, and the cutting of a larger age mother tree has poor cell activity and low differentiation ability, and it is difficult to produce callus, therefore, the callus promoting agent is added in the process of cutting treatment, the cutting is soaked in the callus promoting agent, and then cutting is carried out, the cutting of a larger age mother tree can be selected, the source of the Pinus sylvestris var. mongolica cutting is expanded, which is beneficial to the Pinus sylvestris var. mongolica afforestation industry and reduces the cost. The pyrroloquinoline quinone and Danshensu in the callus promoting agent can synergistically improve the activity of aged cells in the cutting and promote the differentiation of the cells, thereby promoting the formation of callus at the cutting incision; meanwhile, a certain amount of sodium lactate and Gleditsia sinensis extract is added in the callus promoting agent, the active oxygen in the cutting cells is regulated, the activity of the cutting cells is synergistically maintained, and the stability of the cutting cells is increased; in addition, the methyl-β-cyclodextrin in the callus promoting agent can also synergistically promote the synthesis and absorption and transport of polysaccharides and nutrients in the cutting with the oxalacetic acid, thereby promoting the metabolism of the cutting of a higher age mother tree, increasing the lignin in the formed callus, making the callus further differentiate into root primordia, and continuously elongating through the cortex to grow out of the body to produce adventitious roots, thereby shortening the time required for the cutting to root.

[0024] Beneficial effects:

[0025] The present application improves the rooting rate of the higher age scion through the rooting treatment, and further improves the survival rate of the scion, so that the scion of the higher age mother tree can also be rooted by cutting, and the range of scion selection of the scot pine is expanded. Therefore, the present application can effectively alleviate the shortage of scot pine seedlings to meet the market demand for scot pine. And the survival ability of the scot pine scion treated by the present application is strong, which can effectively promote the artificial afforestation. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 : The cutting seedlings of example 1 are used for afforestation. DETAILED DESCRIPTION

[0027] The present application will be described in detail below in combination with specific examples:

[0028] Example 1: Cutting of scot pine

[0029] Preparation of callus promoting agent:

[0030] A: After the soapberry is ground, it is added to a 50wt% ethanol solution, the solid-liquid ratio of the soapberry to the ethanol solution is 1:40, and the extraction is carried out under the conditions of ultrasonic power of 300W and extraction temperature of 50℃ for 15min. After the extraction is completed, the clear liquid is taken to obtain the soapberry extract;

[0031] B: 0.5g of danshensu is added to 50ml of 50% ethanol solution, stirred uniformly to obtain a danshensu solution, then 1.2g of methyl-β-cyclodextrin is ground into a paste with 2.4ml of water, and then added to the danshensu solution, and continues to be ground for 1h. After the grinding is completed, it is dried at 40℃ for 1.5h to obtain the inclusion compound;

[0032] C: The inclusion compound is added to 200ml of deionized water, stirred at 250r / min for 5min, then 1g of oxalacetic acid, 0.6g of pyrroloquinoline quinone and 0.6g of sodium lactate are added, and continue to stir until completely dissolved. 5g of soapberry extract is added and mixed uniformly to obtain the callus promoting agent.

[0033] Method for cutting scot pine:

[0034] (1) Seedbed making: set up a cutting bed with a width of 1.2m and a thickness of 0.5m, lay river pebbles on the bottom of the cutting bed, and then lay the cutting substrate compounded by perlite and grass carbon with a mass ratio of 2:1 on the top, and then disinfect the seedbed with 0.5% potassium permanganate solution 7 days before cutting;

[0035] (2) Cutting treatment: the cutting is taken from the tender branch of the semi-lignified lateral branch of the 5-year-old Pinus sylvestris var. mongolica mother tree, the tender branch is trimmed to 12 cm, then the trimmed tender branch is cut from the lower 4 cm position to the base of the branch by oblique cutting, to obtain the cutting, and the 2 cm end of the cutting is soaked in the 10 times diluted 10 times of the callus promoting agent for 25 min, to obtain the treated cutting;

[0036] (3) Cutting: the treated cutting is dried, and is vertically inserted into the cutting bed at a plant spacing of 4 cm x 3 cm, and the cutting depth is 1 / 3 of the length of the cutting;

[0037] (4) Cutting management: after cutting, the seedbed film is immediately covered with an arched plastic film, and the relative humidity in the Pinus sylvestris var. mongolica seedbed is kept at 85%, and the temperature is 20°C.

[0038] Example 2: Pinus sylvestris var. mongolica cutting II

[0039] Preparation of the callus promoting agent:

[0040] A: Gleditsia sinensis is ground and added to a 50wt% ethanol solution, the solid-liquid ratio of Gleditsia sinensis to the ethanol solution is 1:40, under the conditions of an ultrasonic power of 330W and an extraction temperature of 55°C, the Gleditsia sinensis is extracted for 18 min, and then the clear liquid is taken to obtain a Gleditsia sinensis extract;

[0041] B: 0.55g of Danshensu is added to 55ml of a 50% ethanol solution, stirred uniformly to obtain a Danshensu solution, then 1.35g of methyl-β-cyclodextrin is ground into a paste with 2.7ml of water, and then added to the Danshensu solution, and continues to be ground for 1.3h, and then dried at 45°C for 1.8h after grinding to obtain an inclusion compound;

[0042] C: the inclusion compound is added to 220ml of ionized water, stirred at 300r / min for 8min to obtain a Danshensu solution, then 1.1g of oxalacetic acid, 0.7g of pyrroloquinoline quinone and 0.65g of sodium lactate are added, and continue to stir until completely dissolved, then 5.5g of the Gleditsia sinensis extract is added and mixed uniformly to obtain the callus promoting agent.

[0043] Pinus sylvestris var. mongolica cutting method:

[0044] (1) Seedbed preparation: a cutting bed with a width of 1.2m and a thickness of 0.5m is set, river pebbles are laid at the bottom of the cutting bed, and then a cutting substrate composed of perlite and grass carbon with a mass ratio of 2:1 is laid on top, and then the seedbed is disinfected with a 0.5% potassium permanganate solution 7 days before cutting;

[0045] (2) Cutting treatment: the cutting is obtained by cutting the tender branch of 12 cm in length from the 6-year-old mother tree of Pinus sylvestris var. mongolica at the base of the branch, and then soaking the end of the cutting in the 10 times diluted 2 cm of the promoting callus agent for 25 min;

[0046] (3) Cutting: the treated cutting is dried, and then vertically inserted into the cutting bed at a plant spacing of 4 cm x 3 cm, and the cutting depth is 1 / 3 of the length of the cutting;

[0047] (4) Cutting management: after the cutting is completed, the seedbed film is immediately covered with an arched plastic film, and the relative humidity in the Pinus sylvestris var. mongolica seedbed is maintained at 85%, and the temperature is 20°C.

[0048] Example 3: Pinus sylvestris var. mongolica cutting

[0049] Preparation of the promoting callus agent:

[0050] A: The hickory is ground and added to a 50wt% ethanol solution, the solid-liquid ratio of hickory to ethanol solution is 1:40, under the conditions of ultrasonic power of 350W and extraction temperature of 60℃, the extraction is carried out for 20min, after the extraction is completed, the clear liquid is taken, and the hickory extract is obtained;

[0051] B: 0.6g of Danshensu is added to 60ml of 50% ethanol solution, stirred uniformly, Danshensu solution is obtained, then 1.5g of methyl-β-cyclodextrin is ground into paste with 3ml of water, then added to the Danshensu solution, and continue to grind for 1.5h, after grinding is completed, dry at 50℃ for 2h, and the inclusion complex is obtained;

[0052] C: The inclusion complex is added to 240ml of ionized water, stirred at 350r / min for 10min, and the Danshensu solution is obtained, then 1.2g of oxalacetic acid, 0.8g of pyrroloquinoline quinone, and 0.7g of sodium lactate are added, continue to stir until completely dissolved, then add 6g of hickory extract, mix uniformly, and the promoting callus agent is obtained.

[0053] Pinus sylvestris var. mongolica cutting method:

[0054] (1) Seedbed preparation: set the cutting bed with a width of 1.2m and a thickness of 0.5m, the cutting bed is paved with river pebbles, and then the cutting bed is paved with the cutting substrate compounded by perlite and grass carbon with a mass ratio of 2:1, then the seedbed is disinfected with 0.5% potassium permanganate solution 10 days before cutting;

[0055] (2) Cutting treatment: the cutting is taken from the tender branch of the semi-lignified side branch of the 7-year-old Pinus sylvestris var. mongolica mother tree, the tender branch is trimmed to 15 cm, then the trimmed tender branch is cut from the lower 4 cm position to the base of the branch by oblique cutting, to obtain the cutting, and the 3 cm end of the cutting is soaked in the 10 times diluted callus promoting agent for 50 min, to obtain the treated cutting;

[0056] (3) Cutting: the treated cutting is dried, and is vertically inserted into the cutting bed according to the plant row spacing of 4 cm x 3 cm, and the cutting depth is 1 / 3 of the length of the cutting;

[0057] (4) Cutting management: after the cutting is completed, the film of the seedbed is immediately covered with an arched plastic film, and the relative humidity in the Pinus sylvestris var. mongolica seedbed is kept at 85% and the temperature is kept at 20°C during the cutting seedling cultivation.

[0058] Comparative Example 1

[0059] This comparative example is compared with Example 1, and the difference lies in that the raw material of the callus promoting agent is different, specifically, no pyrroloquinoline quinone is added in the preparation process of the callus promoting agent, and the specific method is as follows:

[0060] A: Gleditsia sinensis is ground and added to a 50wt% ethanol solution, the solid-liquid ratio of Gleditsia sinensis to the ethanol solution is 1:40, under the conditions of ultrasonic power of 300W and extraction temperature of 50°C, the extraction is carried out for 15 min, and after the extraction is completed, the clear liquid is taken to obtain the Gleditsia sinensis extract;

[0061] B: 0.5g of Danshensu is added to 50ml of 50% ethanol solution, stirred uniformly to obtain a Danshensu solution, then 1.2g of methyl-β-cyclodextrin is ground into a paste with 2.4ml of water, and then added to the Danshensu solution, and continues to be ground for 1h, and after the grinding is completed, it is dried at 40°C for 1.5h to obtain an inclusion compound;

[0062] C: the inclusion compound is added to 200ml of deionized water, stirred at 250r / min for 5min, then 1g of oxalacetic acid and 0.6g of sodium lactate are added, and continue to stir until completely dissolved, then add 5g of Gleditsia sinensis extract, and mix uniformly to obtain the callus promoting agent.

[0063] The cutting method used in this comparative example is the same as that of Example 1.

[0064] Comparative Example 2

[0065] This comparative example is compared with Example 1, and the difference lies in that the raw material of the callus promoting agent is different, specifically, no Danshensu is added in the preparation process of the callus promoting agent, and no Danshensu is packaged, and the specific method is as follows:

[0066] A: Gleditsia sinensis L. was ground and added to a 50wt% ethanol solution, the material-liquid ratio of Gleditsia sinensis L. to the ethanol solution was 1:40, under the conditions of ultrasonic power of 300W and extraction temperature of 50℃, extraction was carried out for 15min, and after the extraction was completed, the supernatant was taken to obtain a Gleditsia sinensis L. extract;

[0067] B: The inclusion compound was added to 200ml of deionized water, stirred at 250r / min for 5min, then 1g of oxalacetic acid, 0.6g of pyrroloquinoline quinone, 0.6g of sodium lactate, and 1.2g of methyl-β-cyclodextrin were added, and stirring was continued until complete dissolution, 5g of the Gleditsia sinensis L. extract was added, and mixed uniformly to obtain a wound healing promoting agent.

[0068] The cutting method used in this comparative example was the same as that of Example 1.

[0069] Comparative Example 3:

[0070] This comparative example was compared with Example 1, and the difference was that the raw materials of the wound healing promoting agent were different, specifically, no Gleditsia sinensis L. extract was added in the process of preparing the wound healing promoting agent, and the specific method was as follows:

[0071] A: 0.5g of danshensu was added to 50ml of a 50% ethanol solution and stirred uniformly to obtain a danshensu solution, then 1.2g of methyl-β-cyclodextrin was ground into a paste with 2.4ml of water and added to the danshensu solution, and continued to be ground for 1h, and after the grinding was completed, it was dried at 40℃ for 1.5h to obtain an inclusion compound;

[0072] B: The inclusion compound was added to 200ml of deionized water, stirred at 250r / min for 5min, then 1g of oxalacetic acid, 0.6g of pyrroloquinoline quinone, and 0.6g of sodium lactate were added, and stirring was continued until complete dissolution to obtain a wound healing promoting agent.

[0073] The cutting method used in this comparative example was the same as that of Example 1.

[0074] Comparative Example 4:

[0075] This comparative example was compared with Example 1, and the difference was that the raw materials of the wound healing promoting agent were different, specifically, no oxalacetic acid was added in the process of preparing the wound healing promoting agent, and the specific method was as follows:

[0076] A: Gleditsia sinensis L. was ground and added to a 50wt% ethanol solution, the material-liquid ratio of Gleditsia sinensis L. to the ethanol solution was 1:40, under the conditions of ultrasonic power of 300W and extraction temperature of 50℃, extraction was carried out for 15min, and after the extraction was completed, the supernatant was taken to obtain a Gleditsia sinensis L. extract;

[0077] B: 0.5 g Danshensu was added into 50 ml 50% ethanol solution and stirred to obtain a Danshensu solution, then 1.2 g methyl-β-cyclodextrin was grinded into paste with 2.4 ml water, and then the Danshensu solution was added and grinded for 1 h, and after grinded, the mixture was dried at 40 °C for 1.5 h to obtain the inclusion compound;

[0078] C: The inclusion compound was added into 200 ml deionized water and stirred at 250 r / min for 5 min, then 0.6 g pyrroloquinoline quinone and 0.6 g sodium lactate were added and stirred until completely dissolved, and then 5 g Gleditsia sinensis extract was added and mixed to obtain the wound healing promoting agent.

[0079] The cutting method used in the present comparative example is the same as that in Example 1.

[0080] Comparative Example 5:

[0081] The present comparative example is compared with Example 1, and the difference is that the raw material of the wound healing promoting agent is different, specifically, Danshensu is not included in the preparation of the wound healing promoting agent, and the specific method is as follows:

[0082] A: Gleditsia sinensis was grinded and added into 50 wt% ethanol solution, and the solid-liquid ratio of Gleditsia sinensis to ethanol solution was 1:40, and the extraction was carried out at an ultrasonic power of 300 W and an extraction temperature of 50 °C for 15 min, and then the clear liquid was taken to obtain the Gleditsia sinensis extract;

[0083] C: 0.5 g Danshensu was added into 200 ml 50% ethanol solution and stirred at 250 r / min for 5 min, then 1 g oxalacetic acid, 0.6 g pyrroloquinoline quinone, 0.6 g sodium lactate and 1.2 g methyl-β-cyclodextrin were added and stirred until completely dissolved, and then 5 g Gleditsia sinensis extract was added and mixed to obtain the wound healing promoting agent.

[0084] Comparative Example 6:

[0085] The present comparative example is compared with Example 1, and the difference is that the cuttings of Pinus sylvestris are not treated with the wound healing promoting agent, and the specific method is as follows:

[0086] (1) The same as Example 1;

[0087] (2) Cutting treatment: the cuttings were taken from the tender branches of 5-year-old Pinus sylvestris mother trees, and the tender branches were cut to 12 cm, and then the cut tender branches were cut from the lower 4 cm position to the base of the branches by oblique cutting to obtain the cuttings;

[0088] (3)-(4) The same as Example 1.

[0089] Comparative Example 7:

[0090] This comparative example is contrasted with Example 1, the only difference being that the cutting is treated according to the prior art, specifically by soaking the cutting in a solution of 300 mg / L naphthalene acetic acid, according to the following procedure:

[0091] (1) The same as Example 1;

[0092] (2) Cutting treatment: The cutting is taken from a tender shoot on a 5-year-old mother tree of Pinus sylvestris var. mongolica, the tender shoot is trimmed to 12 cm, then the trimmed tender shoot is cut from the lower 4 cm or so of the branch to the base of the branch by means of oblique cutting, a cutting is obtained, and the end of the cutting is soaked in a solution of 300 mg / L naphthalene acetic acid for 25 min to obtain a treated cutting;

[0093] (3)-(4) The same as Example 1.

[0094] Experiment:

[0095] Pinus sylvestris var. mongolica cutting experiments were carried out according to the methods of Example 1 and Comparative Examples 1-7, wherein the cuttings were selected from 2-year-old mother trees and 5-year-old mother trees. The cuttings were taken from tender shoots on a 5-year-old mother tree of Pinus sylvestris var. mongolica, the tender shoots were trimmed to 12 cm, then the trimmed tender shoots were cut from the lower 4 cm or so of the branch to the base of the branch by means of oblique cutting, a cutting was obtained, and the end of the cutting was soaked in a solution of 300 mg / L naphthalene acetic acid for 25 min to obtain a treated cutting;

[0096] After cutting, 30 cuttings were randomly selected from each experimental group as the object of data recording and statistics. After cutting, the formation of callus and the rooting of the cuttings were recorded after 30 days. The rooting rate of the cuttings was counted after 3 months of cutting, and the survival rate of the cuttings was counted after 4 months of cutting. The results of the 2-year-old mother tree are shown in Table 1, and the results of the 5-year-old mother tree are shown in Table 2.

[0097] Table 1

[0098]

[0099]

[0100] Table 2

[0101]

[0102] From the analysis of Table 1 and Table 2, it can be seen that:

[0103] 1、Table 1 is 2-year-old mother tree cuttings, Table 2 is 5-year-old mother tree cuttings, the rooting rate and survival rate of 2-year-old mother tree cuttings are greater than 5-year-old, and compared with 2-year-old cuttings, the callus formation time and rooting time of 5-year-old cuttings are longer. This is because as the age of the mother tree increases, the activity of the cutting cells gradually deteriorates, the differentiation ability decreases, it is difficult to produce callus, leading to the gradually weakened rooting ability of the cutting and the decreased survival rate. After using the callus promoting agent, although the rooting time and callus formation time of the 5-year-old cutting in Example 1 are longer than those of the 2-year-old mother tree, the rooting rate is greatly improved compared with the 5-year-old cutting without using the callus promoting agent, and the survival rate is greater than 85%, which shows that the present application can effectively promote the rooting of cuttings of mother trees with larger age and improve the survival rate of cutting, can be used for afforestation, and can reduce the cost.

[0104] Analysis of Table 2 can be obtained:

[0105] 1、Compared with Example 1, Comparative Example 1 does not add pyrroloquinoline quinone in the preparation process of the callus promoting agent, and Comparative Example 2 does not add danshensu, the callus time and rooting time of the cuttings are both longer, which is because the cells of the 5-year-old mother tree cutting are aging, the activity is low, leading to the difficulty in forming callus of the cutting, and it is difficult to root, so the rooting rate and survival rate decrease.

[0106] 2、Compared with Example 1, Comparative Example 2 does not add danshensu in the preparation process of the callus promoting agent, the cells of the 5-year-old cutting are aging, the differentiation activity is poor, leading to difficulty in rooting and low survival rate. Compared with Example 1, Comparative Example 5 does not modify danshensu by inclusion, because the stability of danshensu is poor, leading to a shorter action time and poorer effect on the cutting, thereby leading to the decrease of the rooting rate and survival rate of the cutting. However, compared with Comparative Example 2, Comparative Example 5 adds danshensu, which can activate the cells of the cutting to a certain extent, so the rooting rate is higher than that of Comparative Example 2.

[0107] 3、Compared with Example 1, Comparative Example 3 does not add soapnut extract in the preparation process of the callus promoting agent, the cell activity maintenance ability of the 5-year-old cutting is low, the stability is low, and the cells are easily damaged by external influences, thereby affecting the formation of callus of the cutting, the required time is long, leading to the decrease of the rooting rate, and further leading to the insufficient absorption of water and nutrients of the cutting, and the decrease of the survival rate.

[0108] 4、Compared with Example 1, Comparative Example 4 does not add oxalyl acetic acid in the preparation process of the callus promoting agent, the metabolism of the cells of the 5-year-old cutting is slow, leading to the slow synthesis and absorption of polysaccharides and nutrients in the cutting, the synthesis of lignin in the callus is affected, leading to slow rooting and low rooting rate.

[0109] 5、Comparative Example 6 and Example 1, the cuttings are not treated with the wound healing agent, the cuttings form callus slowly, resulting in a low rooting rate, the cuttings cannot effectively absorb water and nutrients, the growth of the cuttings is limited, and the cuttings are difficult to survive. Comparative Example 7 and Example 1, the cuttings are soaked in a 300 mg / L naphthaleneacetic acid solution, compared with Comparative Example 6, the rooting rate and survival rate are improved, but compared with Example 1, the rooting rate and survival rate of the cuttings are both low, indicating that the use of the naphthaleneacetic acid solution can promote the rooting of the cuttings to a certain extent, but the effect of promoting rooting is not as good as that of Example 1.

[0110] The above examples are only used to illustrate the technical solutions of the present application but not limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the present application, and all should be covered in the scope of the claims of the present application. The technical, shape, and structure parts not described in detail in the present application are well-known technologies.

Claims

1. A method of cutting Scots pine (Pinus sylvestris) characterized in that, The method comprises the following steps: (1) seedbed preparation: set up a cutting bed, lay river pebbles on the bottom layer of the cutting bed, and then lay cutting substrate on the river pebbles; (2) cutting treatment: prune the tender branches to obtain cutting branches, then immerse the end of the cutting branches in a callus-promoting agent, and obtain the treated cutting branches after the immersion is completed; (3) cutting: vertically insert the treated cutting branches into the cutting bed after the treated cutting branches are dried; (4) cutting management: immediately cover the seedbed film with an arched plastic film after the cutting is completed, and maintain the relative humidity and temperature in the seedbed of the Pinus sylvestris var. mongolica seedlings; The callus-promoting agent in the step (2) comprises the following raw materials: oxalacetic acid, Gleditsia sinensis extract, pyrroloquinoline quinone, sodium lactate, danshensu, and methyl-β-cyclodextrin. The proportions of the raw materials of the callus-promoting agent are as follows: The mass ratio of oxalacetic acid, Gleditsia sinensis extract, pyrroloquinoline quinone, sodium lactate, danshensu, and methyl-β-cyclodextrin is (1-1.2):(5-6):(0.6-0.8):(0.6-0.7):(0.5-0.6):(1.2-1.5). The preparation method of the callus-promoting agent is as follows: A: after Gleditsia sinensis is ground, the Gleditsia sinensis is added to a 50wt% ethanol solution, ultrasonic extraction is performed, and then the clear liquid is taken to obtain Gleditsia sinensis extract; B: danshensu is added to a 50% ethanol solution, stirred uniformly to obtain a danshensu solution, then methyl-β-cyclodextrin is ground into a paste with water, the danshensu solution is added, and the grinding is continued for 1-1.5 hours, and then drying is performed at 40-50°C for 1.5-2 hours to obtain an inclusion compound; C: the inclusion compound is added to deionized water, stirred at 250-350 r / min for 5-10 minutes, then oxalacetic acid, pyrroloquinoline quinone, and sodium lactate are added, and the stirring is continued until complete dissolution, then the Gleditsia sinensis extract is added, and the mixture is uniformly mixed to obtain the callus-promoting agent.

2. A method of cutting Scots pine as claimed in claim 1, characterized in that, The cutting substrate in the step (1) is prepared by compounding perlite and grass charcoal at a mass ratio of 2:

1.

3. A method of cutting Scots pine as claimed in claim 2, characterized in that, The length of the cutting branches for immersion in the step (2) is 2-3 cm above the incision.

4. A method of cutting Scots pine as claimed in claim 3, characterized in that, The time for the cutting branches to be immersed in the step (2) is 25-35 minutes.

5. A method of cutting Scots pine as claimed in claim 4, characterized in that, In the preparation method of the callus-promoting agent, the solid-liquid ratio of Gleditsia sinensis to the ethanol solution in step A is 1:40, the ultrasonic extraction power of Gleditsia sinensis is 300-350 W, the extraction temperature is 50-60°C, and the extraction time is 15-20 minutes.

6. A method of cuttage of dwarf pine as claimed in any one of the claims 1-5, characterized in that, The mother tree of the Pinus sylvestris var. mongolica cutting branches has a tree age of 2-10 years.

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