Deep-sea animal culture system and methods for achieving constant pressure transfer, feeding, and wastewater discharge using this system.

Abstract

This invention relates to a deep-sea animal culture system and a method for constant-pressure transfer, feeding, and wastewater discharge using this system. The deep-sea animal culture system is housed within a container and includes a culture chamber, a water supply pipeline connected to the culture chamber, a temperature-controlled sleeve on the outer wall of the culture chamber, and a transmission rod passing through the culture chamber. A metabolite removal disc is fixedly connected to the transmission rod, and a driving disc is rotatably connected to it. The water supply pipeline includes: an input section with at least two on / off valves, an output section with at least two on / off valves, and a water supply and pressurization section connected to a water pump. The water supply and pressurization section extends from the water pump to the input section, the culture chamber, and the output section. This invention solves the long-term culture problem of deep-sea macroorganisms (such as lionfish and amphipods) in the laboratory.

Description

Technical Field

[0001] This invention relates to the field of deep-sea culture equipment technology, and in particular to a deep-sea animal culture system and a method for achieving constant pressure transfer, feeding, and sewage discharge using this system. Background Technology

[0002] Research on deep-sea organisms requires long-term continuity. Currently, there are two approaches in China: one is to build in-situ experimental devices on the deep-sea seabed to culture organisms and conduct long-term observation and research. This method is expensive, and once the experimental device is started, researchers have limited intervention power, which can easily lead to experimental failure. The other approach is to build culture chambers in the laboratory, collect biological samples from the seabed, and then transfer them to the culture chambers for long-term culture and observation. This method is less expensive and easier to intervene in, so it is the mainstream direction of deep-sea biological research.

[0003] In China, long-term cultivation of deep-sea microorganisms (such as bacteria) in laboratory culture chambers has been achieved. However, for deep-sea macroorganisms, such as lionfish and amphipods, the relevant technologies for long-term cultivation in laboratories have not yet been solved in China. Summary of the Invention

[0004] In response to the shortcomings of the existing production technologies, the applicant provides a well-structured deep-sea animal culture system and a method for using this system to achieve constant-pressure transfer of deep-sea macroorganisms, feeding into the culture chamber, and discharging wastewater from the culture chamber, which can solve the survival problem of long-term continuous culture of deep-sea macroorganisms.

[0005] The technical solution adopted in this invention is as follows:

[0006] A deep-sea animal culture system, housed within a container, includes a culture chamber, water supply pipes connected to the culture chamber, a temperature-controlled sleeve on the outer wall of the culture chamber, a transmission rod running through the culture chamber, a metabolite removal disc fixedly connected to the transmission rod, and a driving disc rotatably connected to the transmission rod; an opening at the bottom of the culture chamber connects to the external environment.

[0007] The water supply pipeline includes:

[0008] The input section has at least two switching valves.

[0009] The output section has at least two switching valves.

[0010] The water supply and pressurization section is connected to a water pump. From the water pump, the output end of the water supply and pressurization section extends to the input section, the culture chamber, and the output section.

[0011] As a further improvement to the above technical solution:

[0012] The metabolite removal disc is located at the bottom of the culture chamber, with several spokes radiating outwards from the center.

[0013] The driving disc and the transmission rod are threaded together. A guide rod is inserted at an eccentric position on the driving disc, and the guide rod is parallel to the transmission rod and connected to the inner wall of the culture chamber.

[0014] The driving disc has through holes.

[0015] The valves on the input section are connected in series, and the valves on the output section are connected in series.

[0016] The input section is equipped with a first switching valve and a second switching valve.

[0017] The output section is equipped with a third switching valve and a fourth switching valve.

[0018] The water supply and pressurization section is provided in three parts, which are connected to:

[0019] The water pump and the input section, with the water supply and pressurization point located between the on / off valves in the input section,

[0020] The water pump and the output section, with the water pressurization point located between the on / off valves in the output section.

[0021] Water pump and culture chamber

[0022] Each water supply and pressurization section is equipped with a check valve; the three water supply and pressurization sections are respectively equipped with a third check valve, a first check valve, and a second check valve.

[0023] Cavities are provided between the switching valves in the input section and between the switching valves in the output section.

[0024] A constant-pressure sample transfer method using a deep-sea animal culture system includes the following steps:

[0025] Start the motor, which drives the transmission rod to rotate, causing the drive plate to move along the guide rod until it comes into contact with the top end cover of the culture chamber.

[0026] Start the water pump to pump in-situ deep-sea seawater through the pressurization section between the pump and the culture chamber, injecting it into the culture chamber. Once the pressure in the culture chamber has been reduced to a low level, stop the water pump.

[0027] The air conditioning is turned on to raise or lower the temperature inside the container to the desired level. A temperature control sleeve is used to maintain the water temperature inside the culture chamber at the target temperature.

[0028] Start the water pump to pressurize the seawater in the culture chamber to the target pressure.

[0029] The external high-fidelity sampling device is tightly connected to the first switch valve of the input section. The water pump is started, and seawater is injected into the cavity between the two switch valves on the input section until the pressure in the cavity is equal to the pressure inside the culture chamber. The high-fidelity sampling device and the second switch valve are then opened. Under the action of gravity, the contents of the high-fidelity sampling device fall into the culture chamber through the through holes on the switch valve assembly and the driving plate, and are located between the driving plate and the metabolite removal plate. At this time, the second switch valve of the input section is closed.

[0030] A constant-pressure feeding method using a deep-sea animal culture system includes the following steps:

[0031] The material is fed into the cavity on the input section, and the first switch valve is closed.

[0032] Start the water pump to pressurize the cavity between the third check valve and the input section until the pressure inside the cavity is equal to the seawater pressure inside the culture chamber; open the second switch valve, and under gravity, the material enters the space between the driving disc and the metabolite removal disc.

[0033] Close the second switch valve near the culture chamber and open the first switch valve.

[0034] A constant-pressure wastewater discharge method using a deep-sea animal culture system includes the following steps:

[0035] The motor rotates in both directions, driving the metabolite removal disc to rotate via a transmission rod. Under the action of spokes and centrifugal force, the metabolites are pushed into the holes of the culture chamber.

[0036] The water pump is started, and the in-situ seawater is sent to the cavity between the first check valve and the output section, and pressurized to a pressure equivalent to that of the seawater inside the culture chamber.

[0037] Open the third switch valve on the output section near the culture chamber. Under the influence of gravity, the metabolites enter the cavity between the third and fourth switch valves.

[0038] Close the third switch valve near the culture chamber and open the fourth switch valve. Under the influence of gravity, the metabolites are discharged.

[0039] The above steps also apply to removing a portion of the organism from the culture chamber, as follows:

[0040] Close the fourth switch valve and open the third switch valve located on the side closest to the culture chamber.

[0041] The motor is started, driving the transmission rod to rotate. The driving disc moves downward along the guide rod, driving the organisms into the space between the spokes of the metabolic waste removal disc. At the same time, under the driving force of the rotating metabolic waste removal disc, the driving disc itself, and the downward water flow, the organisms enter the cavity of the output section.

[0042] Close the third switch valve on the side closest to the culture chamber, open the fourth switch valve, and remove the organism.

[0043] As a further improvement to the above technical solution:

[0044] A low-light camera is installed inside the culture chamber to monitor the status of macroorganisms inside the chamber over a long period of time.

[0045] The beneficial effects of the present invention are as follows:

[0046] This invention has a compact and reasonable structure and is easy to operate. It solves the problems of constant pressure transfer of deep-sea macroorganisms, as well as the feeding and metabolite removal necessary for the long-term survival of macroorganisms. Attached Figure Description

[0047] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0048] Figure 2 This is a schematic diagram of the driving disc structure of the present invention.

[0049] Figure 3 This is a schematic diagram of the metabolite removal disc structure of the present invention.

[0050] The components include: 1. Container; 2. Air conditioner; 5. Low-light camera; 6. Driving disc; 7. Transmission rod; 8. Metabolic waste removal disc; 9. Motor; 10. Guide rod; 11. Temperature control sleeve; 12. Water pump;

[0051] 3. Culture chamber; 301. Drainage port;

[0052] 401. First switching valve; 402. Second switching valve; 403. Third switching valve; 404. Fourth switching valve;

[0053] 131. First check valve; 132. Second check valve; 133. Third check valve;

[0054] 601. Input through hole;

[0055] 801. Spokes. Detailed Implementation

[0056] The specific embodiments of the present invention will now be described with reference to the accompanying drawings.

[0057] like Figures 1-3 As shown, the deep-sea animal culture system of this embodiment is built into container 1, including culture chamber 3, water supply pipe connected to culture chamber 3, temperature control sleeve 11 on the outer wall of culture chamber 3, transmission rod 7 passing through culture chamber 3, metabolite removal plate 8 fixedly connected to transmission rod 7 and drive plate 6 rotatably connected to transmission rod 7, and hole 301 is opened at the bottom of culture chamber 3, which is connected to the third switch valve 403;

[0058] The water supply pipeline includes:

[0059] The input section has at least two switching valves.

[0060] The output section has at least two switching valves.

[0061] The water supply and pressurization section is connected to a water pump 12. The water supply and pressurization section extends from the water pump 12 to the input section, the culture chamber 3, and the output section.

[0062] Metabolite removal plate 8 is located at the bottom of culture chamber 3, with several spokes radiating outwards from the center.

[0063] The driving disc 6 is threadedly connected to the transmission rod 7. A guide rod 10 is inserted through the driving disc 6 at an eccentric position. The guide rod 10 is parallel to the transmission rod 7 and connected to the inner wall of the culture chamber 3.

[0064] The driving disc 6 has a through hole.

[0065] The switching valves on the input section are connected in series, and the switching valves on the output section are connected in series.

[0066] The water supply pressurization section is set up in three sections, which are connected to:

[0067] Water pump 12 and the input section, the water supply and pressurization position is between the on / off valve of the input section.

[0068] The water pump 12 and the output section, with the water supply and pressurization point located between the on / off valves in the output section,

[0069] Water pump 12 and culture chamber 3,

[0070] Each section of the water supply pressurization section is equipped with a check valve.

[0071] The constant-pressure sample transfer method using a deep-sea animal culture system in this embodiment includes the following steps:

[0072] Start motor 9, which drives transmission rod 7 to rotate. Drive plate 6 moves along guide rod 10 until drive plate 6 abuts against the top end cover of culture chamber 3.

[0073] Start water pump 12 to pump deep-sea seawater through the pressurization section between water pump 12 and culture tank 3 into culture tank 3. After the pressure in culture tank 3 is increased to a low level, stop water pump 12.

[0074] Air conditioner 2 is activated to raise or lower the internal temperature of container 1 to the desired temperature. Temperature control sleeve 11 is used to control the water temperature inside culture chamber 3 to the target temperature.

[0075] Start water pump 12 to pressurize the seawater in culture chamber 3 to the target pressure.

[0076] The external high-fidelity sampling device is in close contact with the first switch valve 401 of the input section. The water pump 12 is started, and seawater is injected into the cavity between the two switch valves on the input section until the pressure in the cavity is equal to the pressure inside the culture chamber 3. The high-fidelity sampling device and the second switch valve 402 are then opened. Under the action of gravity, the animal in the high-fidelity sampling device falls into the culture chamber 3 through the switch valve group and the hole on the driving plate 6, and is located between the driving plate 6 and the metabolite removal plate 8. At this time, the second switch valve 402 of the input section is closed.

[0077] The constant-pressure feeding method using a deep-sea animal culture system in this embodiment includes the following steps:

[0078] The material is fed into the cavity on the input section, and the first switch valve 401 is closed.

[0079] Start water pump 12 to pressurize the cavity between the third check valve 133 and the input section until the pressure inside the cavity is equivalent to the seawater pressure inside the culture chamber 3; open the second switch valve 402, and under the action of gravity, the material enters the space between the driving plate 6 and the metabolite removal plate 8.

[0080] Close the second switch valve 402 near the culture chamber 3 and open the first switch valve 401.

[0081] The wastewater discharge method using a deep-sea animal culture system in this embodiment includes the following steps:

[0082] The motor 9 is started to rotate in both directions, which drives the metabolite removal disk 8 to rotate via the transmission rod 7. Under the action of the spokes and centrifugal force, the metabolites are pushed into the upper hole 301 on the culture chamber 3.

[0083] When water pump 12 is started, the in-situ seawater is sent to the cavity between the first check valve 131 and the output section, and pressurized to a pressure equivalent to that of the seawater inside the culture chamber 3.

[0084] Open the third switch valve 403 on the output section near the culture chamber 3. Under the action of gravity, metabolites enter the cavity between the third switch valve 403 and the fourth switch valve 404.

[0085] When the third switch valve 403 is closed and the fourth switch valve 404 is opened, the metabolites are discharged under the action of gravity.

[0086] The above steps also apply to removing a portion of the organism from culture chamber 3. The specific steps are as follows:

[0087] Close the fourth switch valve 404 and open the third switch valve 403, which is located near the culture chamber 3.

[0088] The motor 9 is started, which drives the transmission rod 7 to rotate. The driving disc 6 moves downward along the guide rod 10, driving the organisms into the space between the spokes of the metabolic waste removal disc 8. At the same time, under the driving force of the rotating metabolic waste removal disc 8 and the downward movement of the water flow from the driving disc 6, the organisms enter the cavity of the output section.

[0089] Close the third switch valve 403 on the side closest to the culture chamber 3, open the fourth switch valve 404, and remove the organism.

[0090] A low-light camera 5 is installed inside the culture chamber 3 to monitor the macro-organisms inside the culture chamber 3 over a long period of time.

[0091] The specific structure of this invention and the operational steps for each stage of culturing the organism are as follows:

[0092] The deep-sea animal culture system and its culture method provided by this invention are particularly suitable for deep-sea macroorganisms. For ease of underwater use and installation, all equipment is installed inside container 1. The culture chamber 3 has a cylindrical structure with a hole 301 at its bottom, connected to a first switching valve 401 for discharging metabolites and sampling organisms. A temperature control sleeve 11 is installed outside the culture chamber 3. The temperature control sleeve 11 uses oil heating to achieve temperature control.

[0093] like Figure 1 As shown, a transmission rod 7 is installed at the axial position inside the culture chamber 1. According to the orientation in the figure, the bottom end of the transmission rod 7 extends out of the culture chamber 3 and is connected to a motor 9; the other end of the transmission rod 7 extends to a position near the top wall of the culture chamber 3. The motor 9 can drive the transmission rod 7 to rotate in both directions.

[0094] A metabolic waste removal disc 8 and a driving disc 6 are fitted onto the transmission rod 7. The metabolic waste removal disc 8 is fixedly connected to the transmission rod 7, and the driving disc 6 is threadedly connected to the transmission rod 7. A guide rod 10, parallel to the transmission rod 7, passes through the driving disc 6 at an eccentric position. When the motor 9 rotates in both directions, the driving disc 6 can reciprocate along the transmission rod 7 and the guide rod 10.

[0095] Structure of Metabolite Removal Disc 8 Figure 3 As shown, spokes 801 radiate evenly outward from the center, with a fan-shaped area enclosed by two spokes 801. A low-light camera 5 is mounted on the upper surface of the metabolite removal disk 8.

[0096] The structure of the driving disc 6 is as follows Figure 2 As shown, the driving plate 6 is provided with several input through holes 601. The function of the input through holes 601 is to allow the bait, organisms, etc. that enter at the input section to smoothly enter the culture chamber 3.

[0097] The diameter of the input through hole 601 is slightly larger than the size of the deep-sea animal, and the hole on the culture chamber 3 that is connected to the second switch valve 402 needs to be concentric with the input through hole 601.

[0098] Viewed from the top of the culture chamber 3, the output through hole 301 on the bottom end cover of the culture chamber 3, which is connected to the third switch valve 403, is located at the end of the spoke of the metabolite removal disk 8 and is located within the rim of the metabolite removal disk 8.

[0099] The inner surface of container 1 needs to be covered with an insulation layer.

[0100] A water pump 12 is installed inside container 1, which draws in seawater from the outside environment. An input section and an output section are led out from the top and bottom of the culture chamber 3, respectively. A first switch valve 401 and a second switch valve 402 are connected in series on the input section, and a third switch valve 403 and a fourth switch valve 404 are connected in series on the output section.

[0101] A cavity is formed between the first switching valve 401 and the second switching valve 402; a cavity is formed between the third switching valve 403 and the fourth switching valve 404.

[0102] Three water supply and pressurization sections are led out from the water pump 12. The three water supply and pressurization sections are respectively equipped with a first check valve 131, a second check valve 132, and a third check valve 133. The first check valve 131 is connected to the cavity between the third switch valve 403 and the fourth switch valve 404, the second check valve 132 is connected to the bottom of the culture chamber 3, and the third check valve 133 is connected to the cavity between the first switch valve 401 and the second switch valve 402.

[0103] The first check valve 131, the second check valve 132, and the third check valve 133 form a structure that is connected in parallel with the water pump 12.

[0104] The above-mentioned structure constitutes a complete culture device, which also has functions of biological constant pressure transfer, feeding, and decontamination.

[0105] The cultivation method includes five processes: deep-sea animal fidelity transfer, feed delivery, animal behavior monitoring, metabolite removal, and animal sampling.

[0106] The steps for ensuring fidelity transfer are as follows:

[0107] S1: Place container 1 on the research vessel;

[0108] S2: Motor 9 starts, driving transmission rod 7 to rotate, thereby causing drive plate 7 to move upward along guide rod 10 until the upper surface of drive plate 7 and the top end cover of culture chamber 3 come into contact.

[0109] S3: Start water pump 12 to inject deep-sea in-situ seawater into the culture chamber 3 through the second check valve 132 and pressurize it to within 10MPa, then stop water pump 3;

[0110] S4: Start air conditioner 2 to raise the internal temperature of container 1 to 25-30℃ (when the target temperature inside culture chamber 3 is higher than room temperature) or lower it to 12-15℃ (when the target temperature inside culture chamber 3 is lower than room temperature); start the temperature control system on the research vessel to circulate the heat transfer oil through the temperature control sleeve 11, thereby controlling the water temperature inside culture chamber 3 to the target temperature.

[0111] S5: Start water pump 3 to pressurize the seawater in culture chamber 3 to the target pressure;

[0112] S6: Connect the high-fidelity sampling device to the first switch valve 401 in a sealed manner, start the water pump 12, and inject in-situ seawater into the cavity between the high-fidelity sampling device and the second switch valve 402 through the third check valve 133 until the pressure in the cavity is equivalent to the pressure inside the culture chamber 3; open the high-fidelity sampling device and the second switch valve 402, and the deep-sea animals inside, under the action of gravity, enter the space between the driving plate 6 and the metabolite removal plate 8 through the first switch valve 401, the second switch valve 402, and the inlet sluice 601; close the second switch valve 402.

[0113] S1-S6 refers to the timely transfer of animals from the deep-sea Fidelity Sampling Device to the Culture Chamber 3 after obtaining the animals from the deep-sea Fidelity Sampling Device, which solves the problem that deep-sea animals cannot survive for a long time in the Fidelity Transfer Device.

[0114] The bait can be dispensed by following these steps:

[0115] S1: Put the bait into the cavity between the first switch valve 401 and the second switch valve 402, and close the first switch valve 401;

[0116] S2: Start water pump 12 to pressurize the cavity between the first switch valve 401 and the second switch valve 402 until it is equivalent to the seawater pressure inside the culture chamber 3; open the second switch valve 402, and under the action of gravity, the feed enters the space between the driving plate 6 and the metabolite removal plate 8;

[0117] S3: Close the second shut-off valve 402 and open the first switch valve 401.

[0118] The S1-S3 method addresses the issue of food supply for the long-term cultivation of deep-sea animals.

[0119] The following steps are used to monitor the behavior of deep-sea animals:

[0120] S1: Periodically activate the low-light camera 5 to take photos of the animals inside the culture chamber 3. Use the photos to monitor the behavior of the animals inside the culture chamber 3 and determine their status, such as whether they are lacking oxygen or whether they need to be fed. Deep-sea animals live in the low-light environment of the deep sea for a long time. The low-light camera can ensure the monitoring of the behavior of the animals inside the culture chamber 3 in the dark.

[0121] Long-term culture of deep-sea animals requires regular removal of metabolites to prevent deterioration of the aquatic environment inside culture chamber 3. The following steps are used to achieve this removal of deep-sea animal metabolites:

[0122] S1: Start the motor 9 to rotate in both directions, and drive the metabolite removal disk 8 to rotate through the transmission rod 7. Under the action of the spokes 801 and centrifugal force, the metabolites are pushed into the output through hole 301.

[0123] S2: Start water pump 12, and seawater enters the cavity between the third switch valve 403 and the fourth switch valve 404 through the first check valve 131, and is pressurized to the same pressure as the seawater inside the culture chamber 3;

[0124] S3: Open the third switch valve 403, and under the action of gravity, the metabolites enter the cavity between the third switch valve 403 and the fourth switch valve 404;

[0125] S4: Close the third switch valve 403 and open the fourth switch valve 404. Under the action of gravity, the metabolites are discharged.

[0126] The long-term culture process of deep-sea animals requires the removal of some organisms from the culture chamber 3 for research, which is achieved through the following steps:

[0127] S1: Close the fourth switch valve 404 and open the third switch valve 403;

[0128] S2: Start motor 9, drive transmission rod 7 to rotate, drive drive disc 6 to move downward along guide rod 10, drive animal into the space between spokes of metabolite removal disc 8, and under the driving action of the rotation of metabolite removal disc 8 and the action of water flow of the downward movement of drive disc 6, animal enters the cavity between third switch valve 403 and fourth switch valve 404.

[0129] S3: Close the third switch valve 403, open the fourth switch valve 404, and remove the animal.

[0130] The advantage of this invention is that it solves the temperature and high pressure conditions required for long-term continuous culture of deep-sea animals, and under these conditions, it enables feeding, sewage discharge, and animal behavior monitoring, ensuring the survival conditions necessary for the animals to survive during the long-term culture process.

[0131] The above description is an explanation of the present invention and not a limitation thereof. The scope of the present invention is defined by the claims. Within the scope of protection of the present invention, any form of modification may be made.

Claims

1. A constant-pressure sample transfer method for a deep-sea animal culture system, characterized in that: The deep-sea animal culture system is built into a container (1); it includes a culture chamber (3) and a water supply pipe connected to the culture chamber (3). The outer wall of the culture chamber (3) is equipped with a temperature control sleeve (11). A transmission rod (7) is installed inside the culture chamber (3). A metabolite removal disc (8) is fixedly connected to the transmission rod (7), and a driving disc (6) is rotatably connected to it. A hole (301) is opened at the bottom of the culture chamber (3), which is connected to the path leading to the external environment. The water supply pipeline includes: The input section has at least two switching valves. The output section has at least two switching valves. The water supply and pressurization section is connected to a water pump (12). From the water pump (12), the output end of the water supply and pressurization section extends to the input section, the culture chamber (3), and the output section, respectively. The driving disc (6) is threadedly connected to the transmission rod (7). A guide rod (10) is inserted through the driving disc (6) at an eccentric position. The guide rod (10) is parallel to the transmission rod (7) and connected to the inner wall of the culture chamber (3). The driving disc (6) has a through hole. The valves on the input section are connected in series, and the valves on the output section are connected in series. The input section is equipped with a first switching valve (401) and a second switching valve (402). The output section is equipped with a third switch valve (403) and a fourth switch valve (404). A constant-pressure sample transfer method for deep-sea animal culture systems includes the following steps: Start the motor (9), which drives the transmission rod (7) to rotate. The driving disc (6) moves along the guide rod (10) until the driving disc (6) comes into contact with the top end cover of the culture chamber (3). Start the water pump (12) to pump deep-sea in-situ seawater through the water delivery and pressurization section between the water pump (12) and the culture chamber (3) into the culture chamber (3). After pressurizing the culture chamber (3) to a low pressure, stop the water pump (12). Turn on the air conditioner (2) to raise or lower the internal temperature of the container (1) to the expected temperature, and use the temperature control sleeve (11) to control the internal water temperature of the culture chamber (3) to the target temperature. Start the water pump (12) to pressurize the seawater in the culture chamber (3) to the target pressure. The external high-fidelity sampling device is in close contact with the first switch valve (401) of the input section. The water pump (12) is started, and seawater is injected into the cavity between the two switch valves on the input section until the pressure of the cavity is equal to the pressure inside the culture chamber (3). The high-fidelity sampling device and the second switch valve (402) are opened. Under the action of gravity, the contents of the high-fidelity sampling device fall into the culture chamber (3) through the through hole on the switch valve group and the driving plate (6) and are located between the driving plate (6) and the metabolite removal plate (8). At this time, the second switch valve (402) of the input section is closed.

2. The constant-pressure sample transfer method for the deep-sea animal culture system as described in claim 1, characterized in that: The metabolite removal plate (8) is located at the bottom of the culture chamber (3), with several spokes (801) radiating outward from the center.

3. The constant-pressure sample transfer method for the deep-sea animal culture system as described in claim 1, characterized in that: The water supply and pressurization section is provided in three parts, which are connected to: The water pump (12) and the input section, the water supply and pressurization position are between the switch valves in the input section, The water pump (12) and the output section, with the water supply and pressurization position located between the on / off valves of the output section, Water pump (12) and culture chamber (3), Each water supply and pressurization section is equipped with a check valve; the three water supply and pressurization sections are respectively equipped with a third check valve (133), a first check valve (131), and a second check valve (132).

4. The constant-pressure sample transfer method for the deep-sea animal culture system as described in claim 1, characterized in that: Cavities are provided between the switching valves in the input section and between the switching valves in the output section.

5. A constant-pressure feeding method for a deep-sea animal culture system, as described in claim 3, characterized in that... The constant pressure feeding method includes the following steps: The material is fed into the cavity on the input section and the first switch valve (401) is closed. Start the water pump (12) to pressurize the cavity between the third check valve (133) and the input section until the pressure inside the cavity is equal to the seawater pressure inside the culture chamber (3); open the second switch valve (402), and under the action of gravity, the material enters the space between the driving plate (6) and the metabolite removal plate (8). Close the second switch valve (402) on the side near the culture chamber (3) and open the first switch valve (401).

6. A constant-pressure wastewater discharge method for a deep-sea animal culture system, as described in claim 3, characterized in that... The constant pressure sewage discharge method includes the following steps: The start motor (9) rotates in both directions, driving the metabolite removal disc (8) to rotate via the transmission rod (7). Under the action of the spokes and centrifugal force, the metabolites are pushed into the holes (301) of the culture chamber (3). The water pump (12) is started, and the in-situ seawater is sent to the cavity between the first check valve (131) and the output section, and pressurized to a pressure equivalent to that of the seawater inside the culture chamber (3). Open the third switch valve (403) on the output section near the culture chamber (3). Under the action of gravity, the metabolites enter the cavity between the third switch valve (403) and the fourth switch valve (404). Close the third switch valve (403) on the side near the culture chamber (3), open the fourth switch valve (404), and the metabolites are discharged under the action of gravity; The above steps also apply to removing a portion of the organism from the culture chamber (3), and the specific steps are as follows: Close the fourth switch valve (404) and open the third switch valve (403) on the side near the culture chamber (3). Start the motor (9), which drives the transmission rod (7) to rotate. The driving disc (6) moves down along the guide rod (10), driving the organisms into the space between the spokes of the metabolite removal disc (8). At the same time, under the driving action of the rotating metabolite removal disc (8), the driving disc (6), and the downward water flow, the organisms enter the cavity of the output section. Close the third switch valve (403) on the side near the culture chamber (3), open the fourth switch valve (404), and remove the organism.

7. The method according to any one of claims 1-6, characterized in that, A low-light camera (5) is installed inside the culture chamber (3) to monitor the macro-organism status inside the culture chamber (3) for a long time.

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