Ultrathin section staining device

Abstract

The present invention provides an ultrathin section staining device, comprising a box body, a blocking mechanism and a placement frame. The box body has a first inner cavity and a second inner cavity, and a discharge port for connecting the outside with the first inner cavity is opened on the outer wall. The placement frame can be used to fix multiple silica gel copper mesh carriers and ultrathin sections thereon, and is arranged in the first inner cavity and can be moved to the second inner cavity. The first inner cavity is used to place acetyl uranyl dye, and the second inner cavity is used to place lead citrate dye. The acetyl uranyl dye in the first inner cavity can isolate the outside air at the discharge port, and the blocking mechanism can discharge the air at the discharge port and block it. The acetyl uranyl dye placed in the first inner cavity of the present invention isolates the discharge port to prevent the outside air from entering, thereby reducing the contact between the lead citrate dye in the second inner cavity and the air, reducing the precipitation generated by the reaction of carbon dioxide in the air with the lead citrate dye, and thus reducing the degree of contamination of the ultrathin section by precipitation.

Description

Technical Field

[0001] The invention relates to the technical field of experimental devices, in particular to an ultrathin section staining device. Background Art

[0002] Transmission electron microscopy ultrathin sections are an important means of studying cell structure and function, especially after the ultrathin sections are stained and then observed under an electron microscope, the organelles and structures are more clearly visible.

[0003] Traditional ultrathin section staining involves attaching the ultrathin sections to a copper grid, dripping uranium salts and lead salts for staining into different areas of the culture dish, usually acetyl uranyl droplets and lead citrate droplets, clamping the copper grid and placing the ultrathin sections on the acetyl uranyl droplets and lead citrate droplets in turn for staining for 15 minutes. After staining, the copper grid is cleaned. Due to the fact that acetyl uranyl droplets are unstable and easy to precipitate under long-term light exposure, and that lead citrate droplets will combine with CO2 in the air to form PbCO3 precipitation, too long a staining time will cause a lot of contamination on the ultrathin sections, affecting observation. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides an ultrathin section staining device, thereby solving or at least alleviating one or more of the above-mentioned problems and other problems in the prior art.

[0005] The present invention provides an ultrathin section staining device, comprising:

[0006] The box body has a first inner cavity and a second inner cavity, and a discharge port for connecting the outside with the first inner cavity is opened on the outer wall;

[0007] A blocking mechanism capable of blocking the discharge port; and

[0008] A placement rack, which can be used to fix multiple silicone copper mesh carriers, is arranged in the first inner cavity and can be moved into the second inner cavity;

[0009] The discharge port can allow the silica gel copper mesh carrier to pass through, and the silica gel copper mesh carrier can carry ultra-thin sections. The first inner cavity is used to place acetyl uranyl dye, and the second inner cavity is used to place lead citrate dye. The acetyl uranyl dye in the first inner cavity can isolate the outside air at the discharge port, and the blocking mechanism can discharge the air at the discharge port.

[0010] Preferably, a third inner cavity is further provided in the box body, the third inner cavity is communicated with the first inner cavity, and the third inner cavity is filled with a first gas that does not react with the lead citrate dye.

[0011] Preferably, the third inner cavity is separated from the second inner cavity by a first partition, and the second inner cavity is separated from the first inner cavity by a second partition, the ends of the first partition and the second partition are connected, and the second partition is provided with an openable and closable window for the placement rack to pass through.

[0012] Preferably, a first sealing ring is provided on a surface of the second partition plate facing the second inner cavity, and the first sealing ring is arranged around the window, and further comprises:

[0013] an electric push rod connected to the inner wall of the second inner cavity; and

[0014] A mounting plate, slidably disposed in the second inner cavity, one surface of which is connected to the output end of the electric push rod, and the other surface of which can abut against the first sealing ring;

[0015] The placement frame is connected to an end of the mounting plate which is away from the electric push rod.

[0016] Preferably, the mounting plate comprises:

[0017] A first plate body, one end surface of which is connected to the electric push rod, and the other end surface of which can abut against the first sealing ring; and

[0018] The second plate body is connected to a surface of the first plate body facing the second partition plate and can be inserted into the window, and its shape matches the window.

[0019] Preferably, a plurality of flow guide holes communicating with the third inner cavity are formed on the side wall of the window.

[0020] Preferably, it is characterized in that the outer wall of the box body corresponding to the third inner cavity is made of elastic material.

[0021] Preferably, a discharge window is provided on the box body, and the discharge window is located at one end of the first inner cavity away from the second partition and on the same side as the third inner cavity. A guide block is connected to the inner wall of the first inner cavity, and the guide block is located between the discharge window and the third inner cavity. A discharge channel is formed between the guide block and the inner wall of the first inner cavity, and the discharge channel and the discharge window form a discharge port.

[0022] Preferably, the blocking mechanism comprises:

[0023] One or more second sealing rings are arranged along the center line direction of the discharge port and connected to the inner peripheral wall of the discharge port; and

[0024] A plugging cylinder, one end of which is closed and can pass through the second sealing ring to be flush with the end of the guide block located in the first inner cavity;

[0025] A first exhaust hole is provided at the closed end of the sealing tube, and a guide plate is connected to the other end of the sealing tube. A guide hole and one or more second exhaust holes are provided on the guide plate. A guide rod which slides along its axial direction is provided in the guide hole, and a pull ring is connected to one end of the guide rod, and the other end of the guide rod is located in the sealing tube and is connected to an elastic sealing plate, which can cover the first exhaust hole. A spring is sleeved on the guide rod, and one end of the spring abuts against the sealing plate, and the other end of the spring abuts against the guide plate.

[0026] Preferably, a side of the guide block facing away from the discharge port is an inclined surface, and the inclined surface faces the junction of the first partition plate and the second partition plate.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] In the technology of the present invention, by setting the first inner cavity and the second inner cavity, and only the first inner cavity is connected with the outside through the discharge port, the acetyl uranyl dye is placed in the first inner cavity to isolate the discharge port, thereby preventing the outside air from entering, thereby reducing the contact between the lead citrate dye in the second inner cavity and the air, reducing the precipitation caused by the reaction of carbon dioxide in the air with the lead citrate dye, thereby reducing the degree of contamination of ultrathin sections by precipitation and improving the staining quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0030] Figure 1 A three-dimensional diagram of an ultrathin section staining device in one embodiment of the present invention;

[0031] Figure 2 for Figure 1 Schematic diagram of the internal structure;

[0032] Figure 3 for Figure 2 Another stereogram of

[0033] Figure 4 for Figure 3 Schematic diagram of the internal structure of the blocking mechanism;

[0034] Figure 5 for Figure 2 Another stereogram of

[0035] Figure 6 for Figure 2Another stereogram of .

[0036] Reference numerals:

[0037] 10. Box body; 11. First inner cavity; 12. Second inner cavity; 13. Discharge port; 131. Discharge window; 132. Discharge channel; 14. Third inner cavity; 15. Guide block;

[0038] 20. Blocking mechanism; 21. Second sealing ring; 22. Blocking cylinder; 23. First exhaust hole; 24. Guide hole; 25. Second exhaust hole; 26. Guide rod; 27. Blocking plate; 28. Spring; 29. ​​Pull ring;

[0039] 30. Mounting frame; 31. Silicone copper mesh carrier board;

[0040] 40. first baffle; 41. second baffle; 411. window; 412. first sealing ring; 413. guide hole;

[0041] 50. Electric push rod; 51. Mounting plate; 511. First plate body; 512. Second plate body. DETAILED DESCRIPTION

[0042] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0043] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0044] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0045] In addition, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, the meaning of "plurality" is more than two, unless otherwise clearly and specifically defined.

[0046] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0047] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0048] See also Figures 1 to 6 This embodiment provides an ultrathin section staining device, including a box body 10, a blocking mechanism 20 and a mounting frame 30.

[0049] The box body 10 has a first inner cavity 11 and a second inner cavity 12, and a discharge port 13 is provided on the outer wall thereof to connect the outside with the first inner cavity 11. The discharge port 13 can allow a silicone copper mesh carrier plate 31 to pass through. The blocking mechanism 20 can block the discharge port 13. The placement rack 30 can be used to fix multiple silicone copper mesh carrier plates 31, which are arranged in the first inner cavity 11 and can be moved to the second inner cavity 12. The silicone copper mesh carrier plate 31 can carry ultrathin sections, the first inner cavity 11 is used to place acetyl uranyl dye, and the second inner cavity 12 is used to place lead citrate dye. The acetyl uranyl dye in the first inner cavity 11 can isolate the outside air at the discharge port 13, and the blocking mechanism 20 can exhaust the air at the discharge port 13. The silicone copper mesh carrier plate 31 is made using existing technology. Specifically, a slit is opened on the silicone plate, and the elasticity of the silicone is used to clamp the copper mesh in the slit, and the copper mesh is purchased as a standard finished product and used to fix the ultrathin sections. In addition, acetyl uranyl stain and lead citrate stain can also be replaced by other types of ultrathin section uranium salt stain and lead salt stain. It should be noted that the uranium salt stain here refers to the type that is easily precipitated by light, and the lead salt stain refers to the type that easily reacts with carbon dioxide.

[0050] In this embodiment, the box body 10 is made of an opaque material. The operator places one or more silica gel copper carriers carrying copper mesh (ultra-thin sections) into the discharge port 13 and places them on the mounting rack 30 for fixing. At this time, the mounting rack 30 is in the first inner cavity 11, and the acetyl uranyl dye immerses the mounting rack 30 to connect the copper mesh and ultra-thin sections thereon. At the same time, the height of the acetyl uranyl dye reaches the discharge port 13, so that during the installation of the silica gel copper mesh carrier 31, the outside air will not enter the first inner cavity 11. Then the blocking mechanism 20 blocks the discharge port 13 and discharges the air there, and the box body 10 is completely isolated inside and outside. After the ultra-thin sections on the copper mesh are dyed in the acetyl uranyl dye in the first inner cavity 11 for a period of time, the mounting rack 30 moves them to the lead citrate dye in the second inner cavity 12 for dyeing. In this process, the acetyl uranyl dye in the first inner cavity 11 and the lead citrate dye in the second inner cavity 12 are isolated from each other through the two inner cavities and will not mix. After the ultrathin sections are stained, they are moved from the second inner cavity 12 to the first inner cavity 11, and the blocking mechanism 20 opens the discharge port 13, and the silica gel copper mesh carrier plate 31 is taken out from the discharge port 13 for subsequent processing. The placement rack 30 can be made of existing technology.

[0051] In the process of dyeing ultrathin sections, the device isolates the outside air by the acetyl uranyl dye in the first inner cavity 11, so that the lead citrate dye in the second inner cavity 12 will not contact and react with carbon dioxide. Since the box body 10 is opaque, the acetyl uranyl dye in the first inner cavity 11 will not precipitate. Both dyes can be stored and used for a long time, thereby reducing costs. The long dyeing time does not cause a lot of pollution on the ultrathin sections, thereby improving the dyeing quality. In addition, the device can use the dye in the first inner cavity 11 to help the dye in the second inner cavity 12 to achieve air isolation, and no more settings are required, so that the structure of the device is simpler and more practical. Compared with the traditional use of sodium hydroxide to absorb carbon dioxide, the device can save the use of sodium hydroxide, further reducing costs, and there is no need to pay attention to the replacement of sodium hydroxide, which is also more convenient.

[0052] In one embodiment, a third inner cavity 14 is further provided in the box body 10, and the third inner cavity 14 is connected to the first inner cavity 11, and the third inner cavity 14 is filled with a first gas that does not react with the lead citrate dye. The first gas can usually be obtained by removing carbon dioxide from air.

[0053] In this embodiment, after the ultrathin sections on the mounting rack 30 are dyed in the first inner cavity 11, the acetyl uranyl dye in the first inner cavity 11 flows into the third inner cavity 14 by rotating the box body 10. At this time, the mounting rack 30 and the ultrathin sections thereon are separated from the acetyl uranyl dye and gradually dried, and then the mounting rack 30 and the ultrathin sections are moved to the second inner cavity 12 and dyed with lead citrate dye. The setting of the third inner cavity 14 can dry the mounting rack 30 together with the ultrathin sections, copper mesh, and silica gel copper mesh carrier plate 31 thereon, and bring as little acetyl uranyl dye into the second inner cavity 12 as possible, which can minimize the loss of acetyl uranyl dye. The setting of the first gas can maintain the pressure balance in the first inner cavity 11 and the third inner cavity 14.

[0054] In one embodiment, the third inner cavity 14 is separated from the second inner cavity 12 by a first partition 40, and the second inner cavity 12 is separated from the first inner cavity 11 by a second partition 41, and the ends of the first partition 40 and the second partition 41 are connected. The second partition 41 is provided with an openable and closable window 411, and the window 411 is used for the placement rack 30 to pass through. Specifically, the first inner cavity 11 and the second inner cavity 12 are parallel to each other, and the second inner cavity 12 is parallel to the third inner cavity 14, and the three are equivalent to the three corners located in a right triangle. The first partition 40 and the second partition 41 are both rectangular parallelepiped, and one end of the two is connected to each other, and the other three ends are connected to the inner wall of the box body 10. In addition, a guide plate can also be provided on the first partition 40, and the end face of the guide plate is inclined, which helps the dye in the third inner cavity 14 to flow back to the first inner cavity 11 when the box body 10 is placed horizontally.

[0055] In this embodiment, Figure 2, the entire box body 10 is placed horizontally, and at this time, there is a silica gel copper mesh carrier plate 31 on the placement rack 30, and there is an ultra-thin section on the copper mesh of the silica gel copper mesh carrier plate 31. The first gas is in the third inner cavity 14 at the upper part. After the ultra-thin section is dyed with acetyl uranyl dye in the first inner cavity 11 for a period of time, the box body 10 is rotated counterclockwise by 90 degrees. At this time, the acetyl uranyl dye in the first inner cavity 11 flows into the third inner cavity 14, and waits for a period of time. After the placement rack 30, the ultra-thin section thereon and the acetyl uranyl dye stained on other structures are separated, the window 411 on the second partition 41 is opened, and the entire placement rack 30 is moved to the second inner cavity 12 through the window 411, and then the window 411 is closed. The ultra-thin section is dyed with the lead citrate dye in the third inner cavity 14. The entire device can be rotated 90 degrees to realize the transfer of the dye in the first inner cavity 11. The box body 10 has a square shape and is easy to control. After the ultrathin sections are stained in the third inner cavity 14, the mounting rack 30 is moved back to the first inner cavity 11, and then the window 411 is closed, and the box body 10 is rotated 90 degrees clockwise, and the acetyl uranyl dye flows back from the third inner cavity 14 to the first inner cavity 11, and the acetyl uranyl dye at this time exceeds the lower end of the discharge port 13, so as to prevent the subsequent blocking mechanism 20 from opening the discharge port 13 and air from entering the first inner cavity 11. Finally, the silica gel copper mesh carrier plate 31 on the mounting rack 30 is removed from the discharge port 13, and the ultrathin sections are removed for subsequent processing.

[0056] The device can prevent the first gas in the third inner cavity 14 from flowing out by returning the acetyl uranyl dye to the first inner cavity 11, and always maintain the air pressure balance in the first inner cavity 11 and the second inner cavity 12. Maintaining the air pressure balance is conducive to the flow of the acetyl uranyl dye, and also prevents the dye from being sucked out due to air pressure imbalance when the window 411 is opened.

[0057] In one embodiment, a first sealing ring 412 is provided on a surface of the second partition plate 41 facing the second inner cavity 12 , and the first sealing ring 412 is arranged around the window 411 .

[0058] The ultrathin section staining device also includes an electric push rod 50 and a mounting plate 51. The electric push rod 50 is connected to the inner wall of the second inner cavity 12. One side of the mounting plate 51 is connected to the output end of the electric push rod 50, and the other side of the mounting plate 51 can be against the first sealing ring 412. The electric push rod 50 can be multi-stage, so that the displacement distance of the mounting plate 51 can be increased. The mounting frame 30 is connected to one end of the mounting plate 51 away from the electric push rod 50. It is slidably arranged in the second inner cavity 12. Specifically, a plurality of guide shafts are arranged in the second inner cavity 12, and a plurality of through holes that slidably cooperate with the guide shafts are opened on the mounting plate 51, so that the mounting plate 51 can slide along the guide shafts to approach or move away from the second partition 41.

[0059] In this embodiment, Figure 5The electric push rod 50 pushes the mounting plate 51 to move rightward and abuts against the first sealing ring 412 on the second partition plate 41 , thereby sealing the window 411 , and at the same time, the mounting frame 30 is moved into the first inner cavity 11 .

[0060] In one embodiment, the mounting plate 51 includes a first plate body 511 and a second plate body 512 .

[0061] The first plate 511 has one end surface connected to the electric push rod 50, and the other end surface can be against the first sealing ring 412. The second plate 512 is connected to the side of the first plate 511 facing the second partition 41, and can be inserted into the window 411. Its shape matches the window 411. Specifically, the second plate 512 is a cuboid, and the window 411 is a rectangular window. The second plate 512 can occupy the inner space of the window 411, thereby reducing the retention amount of the acetyl uranyl dye here when the box body 10 rotates counterclockwise.

[0062] In one embodiment, a plurality of flow guide holes 413 connected to the third inner cavity 14 are provided on the side wall of the window 411. Figure 2 The box body 10 is placed horizontally. After rotating 90 degrees counterclockwise, part of the acetyl uranyl dye is retained in the gap between the second plate body 512 and the window 411. The guide hole 413 on the side wall of the window 411 can flow the dye in the gap back into the third inner cavity 14, thereby reducing the loss of the dye.

[0063] In one embodiment, the outer wall of the box body 10 corresponding to the third inner cavity 14 is made of elastic material. By setting the side wall of the third inner cavity 14 to an elastic material, such as a rubber material, the internal space of the third inner cavity 14 can be expanded and contracted, thereby maintaining the internal air pressure balance when the dye is consumed in the first inner cavity 11 and the second inner cavity 12. In addition, the elastic material of the outer wall of the third inner cavity 14 can also make it possible to fill the gas there, and an elastic filling pad is set there. When the first gas is insufficient and the outer wall of the third inner cavity 14 is shrunk, the first gas is injected into the third inner cavity 14 through a syringe for replenishment.

[0064] In one embodiment, a discharge window 131 is provided on the box body 10. The discharge window 131 is located at one end of the first inner cavity 11 away from the second partition 41 and on the same side as the third inner cavity 14. A guide block 15 is connected to the inner wall of the first inner cavity 11. The guide block 15 is located between the discharge window 131 and the third inner cavity 14. A discharge channel 132 is formed between the guide block 15 and the inner wall of the first inner cavity 11. The discharge channel 132 and the discharge window 131 form a discharge port 13. Specifically, as Figure 2 The upper end, front end and rear end of the guide block 15 are connected to the inner wall of the first inner cavity 11, and the lower end of the guide block 15 is suspended.

[0065] In this embodiment, Figure 2 When the acetyl uranyl dye is located in the first inner cavity 11, its highest point should be immersed in the lower end of the guide block 15, thereby preventing air from entering.

[0066] In one embodiment, the sealing mechanism 20 includes one or more second sealing rings 21 and a sealing cylinder 22 .

[0067] The second sealing ring 21 is disposed along the center line direction of the discharge port 13 and connected to the inner peripheral wall of the discharge port 13 .

[0068] One end of the plugging tube 22 is closed and can pass through the second sealing ring 21 to be flush with the end of the guide block 15 located in the first inner cavity 11. Specifically, the outer wall of the plugging tube 22 is tightly fitted with the inner ring of the second sealing ring 21 to achieve sealing. The cross-section of the plugging tube 22 can be circular or square. Figure 4 The horizontal cross section of the middle plugging cylinder 22 is square.

[0069] A first exhaust hole 23 is provided at the closed end of the sealing tube 22, and a guide plate is connected to the other end of the sealing tube 22, on which a guide hole 24 and one or more second exhaust holes 25 are provided; a guide rod 26 is provided in the guide hole 24 and slides along its axial direction, and a pull ring 29 is connected to one end of the guide rod 26, and the other end of the guide rod 26 is located in the sealing tube 22 and is connected to an elastic sealing plate 27, which can cover the first exhaust hole 23, and a spring 28 is sleeved on the guide rod 26, one end of the spring 28 is against the sealing plate 27, and the other end of the spring 28 is against the guide plate.

[0070] In this embodiment, Figure 4 , pull the pull ring 29 on the plugging cylinder 22, compress the spring 28, and make the plugging plate 27 move away from the first exhaust hole 23 at the closed end. As the plugging cylinder 22 is inserted into the discharge port 13 and passes through the second sealing ring 21, the air below the plugging cylinder 22 flows out from the first exhaust hole 23. Until the lower end of the plugging cylinder 22 is inserted into the acetyl diuranyl dye and is flush with the lower end of the guide block 15, the pull ring 29 is released at this time, and the plugging plate 27 blocks the first exhaust hole 23. The plugging cylinder 22 can be temporarily guaranteed not to be separated from the discharge port 13 by relying on the second sealing ring 21.

[0071] In one embodiment, the side of the guide block 15 facing away from the discharge port 13 is an inclined surface, and the inclined surface faces the junction of the first partition plate 40 and the second partition plate 41. Figure 2 When the box body 10 is placed horizontally, if there is a first gas in the first inner cavity 11, it can enter the third inner cavity 14 along the inclined surface of the guide block 15, thereby reducing the first gas remaining under the guide block 15, and escaping from the discharge port 13 after the blocking mechanism 20 releases the blockage.

[0072] In the description of the present invention, a large number of specific details are described. However, it is understood that embodiments of the present invention can be practiced without these specific details. In some instances, well-known methods, structures and techniques are not shown in detail so as not to obscure the understanding of this description.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. An ultrathin section staining device, characterized in that: include: The box body (10) comprises a first inner cavity (11) and a second inner cavity (12), and a discharge port (13) is provided on the outer wall thereof for connecting the outside with the first inner cavity (11); A blocking mechanism (20) capable of blocking the discharge port (13); and A mounting frame (30) can be used to fix a plurality of silica gel copper mesh carrier plates (31), is arranged in the first inner cavity (11), and can be moved into the second inner cavity (12); The discharge port (13) can allow the silica gel copper mesh carrier plate (31) to pass through, and the silica gel copper mesh carrier plate (31) can carry ultra-thin sections. The first inner cavity (11) is used to place acetyl uranyl dye, and the second inner cavity (12) is used to place lead citrate dye. The acetyl uranyl dye in the first inner cavity (11) can isolate the outside air from the discharge port (13), and the blocking mechanism (20) can discharge the air at the discharge port (13); The box body (10) is further provided with a third inner cavity (14), the third inner cavity (14) being in communication with the first inner cavity (11), and the third inner cavity (14) being filled with a first gas that does not react with the lead citrate dye; The third inner cavity (14) and the second inner cavity (12) are separated by a first partition (40), and the second inner cavity (12) and the first inner cavity (11) are separated by a second partition (41), the first partition (40) and the second partition (41) are connected at their ends, and the second partition (41) is provided with an openable and closable window (411), and the window (411) is used for the placement frame (30) to pass through; A guide block (15) is connected to the inner wall of the first inner cavity (11); When the ultrathin sections on the mounting rack (30) have been stained in the first inner cavity (11), the box body (10) is rotated so that the uranyl acetate stain in the first inner cavity (11) flows into the third inner cavity (14). At this time, the mounting rack (30) and the ultrathin sections thereon are separated from the uranyl acetate stain and gradually dried. Then, the mounting rack 30 and the ultrathin sections are moved into the second inner cavity (12) and stained with the lead citrate stain.

2. An ultrathin section staining device as claimed in claim 1, characterized in that: A first sealing ring (412) is provided on a surface of the second partition plate (41) facing the second inner cavity (12), and the first sealing ring (412) is arranged around the window (411), and further comprises: An electric push rod (50) connected to the inner wall of the second inner cavity (12); and A mounting plate (51) slidably disposed in the second inner cavity (12), one surface of which is connected to the output end of the electric push rod (50), and the other surface of which can abut against the first sealing ring (412); The placement frame (30) is connected to an end of the mounting plate (51) that is away from the electric push rod (50).

3. An ultrathin section staining device as claimed in claim 2, characterized in that: The mounting plate (51) comprises: A first plate body (511), one end surface of which is connected to the electric push rod (50), and the other end surface of which can abut against the first sealing ring (412); and The second plate body (512) is connected to a surface of the first plate body (511) facing the second partition plate (41), and can be inserted into the window (411), and its shape matches the window (411).

4. An ultrathin section staining device as claimed in claim 3, characterized in that: A plurality of flow guide holes (413) communicating with the third inner cavity (14) are provided on the side wall of the window (411).

5. An ultrathin section staining device according to any one of claims 1 to 4, characterized in that: The portion of the outer wall of the box body (10) corresponding to the third inner cavity (14) is made of elastic material.

6. An ultrathin section staining device as claimed in claim 5, characterized in that: The box body (10) is provided with a material discharge window (131), the material discharge window (131) is located at one end of the first inner cavity (11) away from the second partition plate (41) and on the same side as the third inner cavity (14), the guide block (15) is located between the material discharge window (131) and the third inner cavity (14), the guide block (15) and the inner wall of the first inner cavity (11) form a material discharge channel (132), and the material discharge channel (132) and the material discharge window (131) form a material discharge port (13).

7. An ultrathin section staining device as claimed in claim 6, characterized in that: The blocking mechanism (20) comprises: One or more second sealing rings (21) are arranged along the centerline direction of the discharge port (13) and connected to the inner peripheral wall of the discharge port (13); and A sealing cylinder (22) having one end which is closed and can pass through the second sealing ring (21) to be flush with the end of the guide block (15) located in the first inner cavity (11); A first exhaust hole (23) is provided at the closed end of the blocking tube (22), and a guide plate is connected to the other end of the blocking tube (22). A guide hole (24) and one or more second exhaust holes (25) are provided on the guide plate. A guide rod (26) is provided in the guide hole (24) and slides along its axial direction. One end of the guide rod (26) is connected to a pull ring (29). The other end of the guide rod (26) is located in the blocking tube (22) and is connected to an elastic blocking plate (27). The blocking plate (27) can cover the first exhaust hole (23). A spring (28) is sleeved on the guide rod (26), one end of the spring (28) abuts against the blocking plate (27), and the other end of the spring (28) abuts against the guide plate.

8. An ultrathin section staining device as claimed in claim 7, characterized in that: A surface of the guide block (15) facing away from the discharge port (13) is an inclined surface, and the inclined surface faces the junction of the first partition plate (40) and the second partition plate (41).

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