A cell detection device

Abstract

The present invention discloses a cell detection device, and relates to the field of cell detection technology. The cell detection device includes a transmission member arranged inside a transmission frame, which is assembled to move upward so that two arc-shaped clamping plates can move toward or away from each other; after moving downward, a placement plate can be vibrated. The present invention arranges a placement plate, a support frame, a transmission frame and a transmission member. After a culture dish is placed on the placement plate, the transmission member after moving upward can make the arc-shaped clamping plates on it move toward each other, thereby fixing the culture dish. Afterwards, after moving downward, the transmission frame can be driven to vibrate by vibration, thereby vibrating the culture dish on the placement plate, and when adding dye, the placement plate can drive the culture dish to rotate to achieve uniform dyeing, thereby solving the problem that the prior art is inconvenient to fix culture dishes of different sizes, and vibrates the dyed culture dish to achieve rapid fusion dyeing of the dye and the cells, and there is a long dyeing cycle.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, in particular to a cell detection device. Background Art

[0002] The definition of cells has been constantly revised over the years and defined at different levels. Currently, most biologists and medical scientists believe that cells are a type of cells from embryos, fetuses or adults that have the ability to self-renew, proliferate and differentiate unlimitedly under certain conditions. They can produce daughter cells with exactly the same phenotype and genotype as themselves, as well as specialized cells that make up the body's tissues and organs. They can also differentiate into progenitor cells. After cultivating the cells under different conditions, they need to be stained and then observed to see if there are any missing or deformed shapes.

[0003] The Chinese invention patent with authorization announcement number CN114994346B discloses a cell detection device and a detection method thereof. Other devices are installed through a mounting seat, and then a digital microscope detects and compares the cell mixture placed on the observation device. The transmission device and the position adjustment device allow the observation device to rotate while observing, and can rotate to the next pile of cells while magnifying and observing the cells. The operating table drives the inclined block and the observation device to rotate around the center of the operating table, and then the observation device cooperates with the fixed gear, and the cell storage and discharge device cooperates with the inclined block, so that the cell culture fluid inside the cell storage and discharge device is distributed in a circle on the observation device, and then the staining device stains the sampled cells. As the operating table drives the observation device to rotate, the samples in the corresponding cell storage and discharge device are quickly collected on the observation device.

[0004] However, the prior art is inconvenient to fix culture dishes of different sizes while vibrating the stained culture dishes to achieve rapid fusion staining of the dye and cells, and there is a problem of a long staining cycle. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a cell detection device, which solves the problems of the prior art that it is inconvenient to fix culture dishes of different sizes while vibrating the stained culture dishes to achieve rapid fusion staining of the stain and cells, and has a long staining cycle.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a cell detection device, comprising a supporting bottom cover provided with a detection position and a staining position, an electron microscope is fixedly installed at the detection position of the supporting bottom cover, and also comprising: a staining rack detachably installed at the staining position of the supporting bottom cover, an extrusion component for pushing out the dye from the liquid outlet pipe on the staining rack is arranged inside the staining rack; a rotatable supporting frame arranged on the supporting bottom cover, a plurality of rotatable placement plates for placing cell culture dishes are arranged in an array on the supporting frame, and the bottom of each placement plate is fixedly connected to a transmission frame, which is configured to rotate the placement plate when the extrusion component pushes out the dye from the liquid outlet pipe on the staining rack after the placement plate is rotated to the staining position of the staining rack; two arc-shaped clamping plates arranged on the placement plate are used to clamp the culture dish placed on the placement plate, and a transmission member arranged inside the transmission frame, which is configured to cause the two arc-shaped clamping plates to move toward or away from each other after upward movement; and to vibrate the placement plate after downward movement.

[0007] Furthermore, a driving motor is fixedly connected to the bottom of the supporting bottom cover, and a rotating shaft is fixedly connected to the end of the output shaft of the driving motor. The rotating shaft movably passes through the supporting bottom cover and is fixedly connected to the supporting frame, and the bottom of the supporting frame is movably connected to the inner wall of the supporting bottom cover through a connecting bearing; the staining frame is fixedly connected to the supporting frame by screws, and a laser transmitter is fixedly connected to the staining frame, and laser receivers are fixedly connected to the positions corresponding to the placement plates on the supporting frame. When staining is required, after the laser receiver receives the light signal from the laser transmitter, the controller cuts off the power to the driving motor, and the extrusion component pushes the dye out of the liquid outlet pipe on the staining frame, and at the same time, the transmission frame drives the placement plate to rotate, so that the dye is in uniform contact with the cells inside the culture dish.

[0008] Furthermore, the extrusion component includes a driving motor and a threaded rod, the driving motor is fixedly connected to the bottom of the dyeing frame, and its output shaft movably passes through the dyeing frame and is fixedly connected to one end of the threaded rod. A piston is threadedly sleeved on the surface of the threaded rod, and a guide block is fixedly connected to the surface of the piston, and the surface of the guide block is movably connected to the inner wall of the dyeing frame.

[0009] Furthermore, a support plate is fixedly connected to the surface of the dyeing frame, a rotating member is fixedly connected to the bottom of the support plate, a transmission pulley is fixedly sleeved on the output shaft of the driving motor, and the rotating member and the transmission pulley are connected through belt transmission; the transmission frame includes a circular tube portion and a support portion, the support portion is fixedly connected to the top of the circular tube portion, the top of the support portion is fixedly connected to the surface of the placement plate, the surface of the circular tube portion is fixedly connected with teeth meshing with the rotating member, and the placement plate is connected to the support frame through a fixed bearing.

[0010] Furthermore, the transmission member includes an intermediate portion, an abutment portion and an adjustment portion, the abutment portion is integrally formed at the top of the intermediate portion, the adjustment portion is integrally formed at the bottom of the intermediate portion, the surface of the intermediate portion is uniformly arrayed with a plurality of external teeth, the interior of the circular tube portion is uniformly arrayed with a plurality of internal teeth, the surface of the external teeth is movably connected to the surface of the internal teeth; a gear-moving member is provided on the abutment portion, after the rotating member moves upward, the external teeth are disengaged from the internal teeth, and the adjustment portion drives the rotating member to rotate, so that the gear-moving member on the abutment portion causes the arc-shaped clamping plates to move apart or toward each other through the transmission member; a connecting spring is fixedly connected to the surface of the abutment portion, and one end of the connecting spring is connected to the interior of the circular tube portion through a limit bearing.

[0011] Furthermore, a vibration-boosting plate is fixedly connected to the interior of the supporting bottom cover on the same side of the detection position and the dyeing position, and both sides of the vibration-boosting plate are provided with inclined surfaces facing the bottom of the supporting bottom cover, and a plurality of vibration points are evenly arrayed on the bottom surface of the vibration-boosting plate, and a plurality of baffles are evenly arrayed and fixed on the top surface of the adjustment part.

[0012] Furthermore, an unlocking position and a fixing position are sequentially arranged inside the supporting bottom cover at the same side of the detecting position and the dyeing position; a rising arc plate is fixedly connected inside the supporting bottom cover at the detecting position and the dyeing position for moving the rotating part upward; an outer tooth plate with adjustable position is arranged on the unlocking position of the supporting bottom cover; a screw is fixedly connected on the surface of the outer tooth plate, one end of the screw thread penetrates through the supporting bottom cover and extends to the outside thereof; a guide rod is also fixedly connected to the surface of the outer tooth plate, and the guide rod is movably connected to the supporting bottom cover; an inner tooth plate is fixedly connected to the fixed position of the supporting bottom cover, and when the adjusting part moves to the position of the inner tooth plate, it rolls on the inner tooth plate to rotate the rotating part, and drives the arc-shaped clamping plates to move toward each other through the gear-moving part on the abutting part.

[0013] Furthermore, the geared part includes an upper gear ring and a lower gear ring, the upper gear ring is connected to the surface of the abutment part through a positioning bearing, and the lower gear ring is slidably connected to the surface of the abutment part through a connecting block, and tooth blocks are arranged on opposite sides of the upper gear ring and the lower gear ring, and the tooth blocks on the upper gear ring and the tooth blocks of the lower gear ring are arranged opposite to each other, and magnet sheets are fixedly connected to the surfaces of the tooth blocks; an abutment spring is fixedly connected to the surface of the lower gear ring, and one end of the abutment spring is fixedly connected to the surface of the abutment part.

[0014] Furthermore, the transmission component includes a screw and a transmission bevel gear, the screw is movably connected to the placement plate, the arc-shaped clamping plate is threadedly sleeved on the surface of the screw, and the transmission bevel gear is fixedly sleeved on the surface of the screw, and the bottom of the placement plate is also movably connected to an active bevel gear and a pressure plate through a connecting shaft, the active bevel gear is meshed with the transmission bevel gear, and the opposite sides of the pressure plate and the upper gear ring are fixedly connected with extrusion teeth.

[0015] Furthermore, an electromagnet is fixedly connected to the position of the support frame corresponding to the placement plate, and a brake component is movably connected to the surface of the placement plate, and a magnet block is fixedly connected to the surface of the brake component. When dyeing is required, after the laser receiver receives the light signal from the laser transmitter, the electromagnet is energized to separate the brake component from the placement plate by adsorbing the magnet block.

[0016] The present invention has the following beneficial effects:

[0017] (1) The cell detection device is provided with a placement plate, a support frame, a transmission frame and a transmission member. After a culture dish is placed on the placement plate, the transmission member can move upward to cause the arc-shaped clamping plates on the placement plate to move toward each other, thereby fixing the culture dish. Afterwards, after moving downward, the transmission frame can be driven to vibrate by vibration, thereby vibrating the culture dish on the placement plate. When the dye is added, the placement plate can drive the culture dish to rotate to achieve uniform dyeing. This solves the problem of the inconvenience of fixing culture dishes of different sizes in the prior art, while vibrating the dyed culture dish to achieve rapid fusion dyeing of the dye and cells, and the problem of a long dyeing cycle.

[0018] (2) The cell detection device is provided with an upper tooth ring and a lower tooth ring, wherein the upper tooth ring and the lower tooth ring are connected by a magnet sheet, and the upper tooth ring and the abutting portion are movably connected, and the lower tooth ring and the abutting portion are slidably connected. When the culture dish causes the arc-shaped clamping plate to rotate, the extrusion force will be concentrated on the extrusion teeth between the upper tooth ring and the pressure plate, and the upper tooth ring and the lower tooth ring are connected by magnetic force. Therefore, when the upper tooth ring cannot rotate and the rotating part at the bottom continues to rotate, the lower tooth ring is pressed and moves downward, and under the action of the abutting spring, it reciprocates up and down to realize the continuous rotation of the rotating part, and the arc-shaped clamping plate will not move, thereby protecting the culture dish. This design is also conducive to clamping culture dishes of different sizes.

[0019] (3) In the cell detection device, the adjustment part contacts the vibration-assisting plate fixed inside the supporting bottom cover, and an inclined surface is provided on the vibration-assisting plate. Under the action of the inclined surface, the adjustment part drives the entire rotating part to move downward. At this time, the connecting spring is compressed to the minimum. A plurality of baffles are evenly arranged in an array on the adjustment part, and a plurality of vibration points are fixedly connected to the vibration-assisting plate. The baffles contact the vibration points and vibrate. According to the density of the baffles and the density of the vibration points, high-frequency and low-amplitude vibration can be achieved, so that the rotating frame is vibrated through the rotating part, and then the placement plate is rotated, which can achieve mixing of the components of the cell fluid and the dye, facilitating rapid dyeing.

[0020] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal structure of the dyeing frame of the present invention;

[0023] Figure 3 It is a structural schematic diagram of the support frame of the present invention;

[0024] Figure 4 This is a schematic diagram of the internal structure of the support frame of the present invention;

[0025] Figure 5 For the present invention Figure 4 A magnified view of the structure at center;

[0026] Figure 6 This is a schematic diagram of the internal structure of the circular tube portion of the present invention;

[0027] Figure 7 It is a schematic diagram of the transmission structure of the present invention;

[0028] Figure 8 This is a schematic diagram of the structure of the extrusion teeth of the present invention;

[0029] Fig. 9 This is a schematic diagram of the internal structure of the support bottom cover of the present invention;

[0030] Fig.10 It is a schematic diagram of the structure of the vibration-boosting plate of the present invention.

[0031] In the figure, 1, supporting bottom cover; 2, dyeing frame; 3, supporting frame; 4, placing plate; 5, transmission frame; 501, round tube part; 502, supporting part; 6, arc clamping plate; 7, transmission member; 701, middle part; 702, abutting part; 703, adjusting part; 8, driving motor; 9, rotating shaft; 10, laser transmitter; 11, laser receiver; 12, driving motor; 13, threaded rod; 14, piston; 15, guide block; 16, supporting plate; 17, rotating member; 18, transmission Pulley; 19, external teeth; 20, internal teeth; 21, connecting spring; 22, vibration-assisting plate; 23, baffle; 24, rising arc plate; 25, external tooth plate; 26, screw; 27, guide rod; 28, internal tooth plate; 29, upper gear ring; 30, lower gear ring; 31, gear block; 32, magnet sheet; 33, abutment spring; 34, screw; 35, transmission bevel gear; 36, active bevel gear; 37, pressure plate; 38, extrusion teeth; 39, electromagnet; 40, brake; 41, magnet block. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0034] See also Figure 1-Figure 10 The embodiment of the present invention provides a technical solution: a cell detection device, comprising a support bottom cover 1 provided with a detection position and a staining position, an electron microscope fixedly installed at the detection position of the support bottom cover 1, and also comprising: a staining frame 2 detachably installed at the staining position of the support bottom cover 1, an extrusion component for pushing out the stain from the liquid outlet pipe on the staining frame 2 is arranged inside the staining frame 2; a rotatable support frame 3 arranged on the support bottom cover 1, a plurality of rotatable placement plates 4 for placing cell culture dishes are arranged in an array on the support frame 3, and a transmission frame 5 is fixedly connected to the bottom of each placement plate 4, which is configured to rotate the placement plate 4 when the extrusion component pushes out the stain from the liquid outlet pipe on the staining frame 2 after the placement plate 4 rotates to the staining position of the staining frame 2; two arc-shaped clamping plates 6 arranged on the placement plate 4, used to clamp the culture dish placed on the placement plate 4, and a transmission member 7 arranged inside the transmission frame 5, which is configured to make the two arc-shaped clamping plates 6 move toward or away from each other after upward movement; after downward movement, the placement plate 4 can vibrate.

[0035] Specifically, a driving motor 8 is fixedly connected to the bottom of the supporting bottom cover 1, and a rotating shaft 9 is fixedly connected to the end of the output shaft of the driving motor 8. The rotating shaft 9 movably penetrates the supporting bottom cover 1 and is fixedly connected to the supporting frame 3. The bottom of the supporting frame 3 is movably connected to the inner wall of the supporting bottom cover 1 through a connecting bearing; the staining frame 2 is fixedly connected to the supporting frame 3 by screws, and a laser transmitter 10 is fixedly connected to the staining frame 2. The positions corresponding to the placement plates 4 on the supporting frame 3 are fixedly connected with laser receivers 11. When staining is required, after the laser receiver 11 receives the optical signal of the laser transmitter 10, the controller cuts off the power to the driving motor 8, and the extrusion component pushes the dye out of the liquid outlet pipe on the staining frame 2. At the same time, the transmission frame 5 drives the placement plate 4 to rotate, so that the dye is in uniform contact with the cells inside the culture dish.

[0036] The extrusion component includes a driving motor 12 and a threaded rod 13. The driving motor 12 is fixedly connected to the bottom of the dyeing frame 2. The output shaft thereof movably penetrates the dyeing frame 2 and is fixedly connected to one end of the threaded rod 13. A piston 14 is threadedly sleeved on the surface of the threaded rod 13. A guide block 15 is fixedly connected to the surface of the piston 14. The surface of the guide block 15 is movably connected to the inner wall of the dyeing frame 2.

[0037] In this embodiment, when one of the placement trays 4 moves to the dyeing position, the laser emitted by the laser transmitter 10 on the dye will be received by the laser receiver 11, and the received light signal will be transmitted to the processor. After processing by the processor, the driving motor 8 is powered off and self-locked through the controller, so that the support frame 3 stops rotating. At this time, the circular tube portion 501 on the rotating member 17 contacts the transmission member, and the teeth on the circular tube portion 501 can engage with the teeth on the transmission member 7. Then the driving motor 12 is started, driving the threaded rod 13 and the transmission pulley 18 on its output shaft to rotate. The rotation of the threaded rod 13 can cause the piston 14 on it to move upward, so that the dye inside the dyeing frame 2 can be pushed out of the dyeing tube.

[0038] Specifically, a support plate 16 is fixedly connected to the surface of the dyeing frame 2, a rotating member 17 is fixedly connected to the bottom of the support plate 16, a transmission pulley 18 is fixedly sleeved on the output shaft of the driving motor 12, and the rotating member 17 is connected to the transmission pulley 18 through belt transmission; the transmission frame 5 includes a circular tube portion 501 and a support portion 502, the support portion 502 is fixedly connected to the top of the circular tube portion 501, the top of the support portion 502 is fixedly connected to the surface of the placement plate 4, the surface of the circular tube portion 501 is fixedly connected with teeth meshing with the rotating member 17, and the placement plate 4 is connected to the support frame 3 through a fixed bearing.

[0039] In this embodiment, the transmission pulley 18 rotates the rotating member 17 through the belt, which can drive the circular tube portion 501 to rotate, and the placement plate 4 can be rotated through the support portion 502 on the circular tube portion 501, and the culture dish can be rotated, so that the dye can be evenly dripped into the culture dish.

[0040] Specifically, the transmission member 7 includes an intermediate portion 701, an abutting portion 702 and an adjusting portion 703. The abutting portion 702 is integrally formed at the top of the intermediate portion 701, and the adjusting portion 703 is integrally formed at the bottom of the intermediate portion 701. The surface of the intermediate portion 701 is uniformly arrayed with a plurality of external teeth 19, and the interior of the circular tube portion 501 is uniformly arrayed with a plurality of internal teeth 20, and the surface of the external teeth 19 is movably connected to the surface of the internal teeth 20; a toothed member is arranged on the abutting portion 702, and after the rotating member 17 moves upward, the external teeth 19 are disengaged from the internal teeth 20, and the adjusting portion 703 drives the rotating member 17 to rotate, so that the toothed member on the abutting portion 702 causes the arc-shaped clamping plate 6 to move away from or toward each other through the transmission component; a connecting spring 21 is fixedly connected to the surface of the abutting portion 702, and one end of the connecting spring 21 is connected to the interior of the circular tube portion 501 through a limit bearing.

[0041] A vibration-boosting plate 22 is fixedly connected to the inside of the supporting bottom cover 1 on the same side of the detection position and the dyeing position. Both sides of the vibration-boosting plate 22 are provided with inclined surfaces facing the bottom of the supporting bottom cover 1. A number of vibration points are evenly arrayed on the bottom surface of the vibration-boosting plate 22, and a number of baffle bars 23 are fixedly and evenly arrayed on the top surface of the adjustment part 703.

[0042] In this embodiment, an inclined surface is provided on the vibration-assisting plate 22. Under the action of the inclined surface, the adjusting portion 703 drives the entire rotating member 17 to move downward. At this time, the connecting spring 21 is compressed to the minimum. A plurality of baffles 23 are evenly arranged in an array on the adjusting member. At the same time, a plurality of vibration points are fixedly connected to the vibration-assisting plate 22. The baffles 23 contact the vibration points and vibrate. High-frequency and low-amplitude vibrations can be achieved according to the density of the baffles 23 and the density of the vibration points, so that the rotating frame is vibrated through the rotating member 17, and then the rotation of the placement plate 4 is achieved, so that the components of the cell fluid and the dye can be mixed, which is convenient for rapid dyeing.

[0043] Specifically, an unlocking position and a fixing position are sequentially arranged inside the supporting bottom cover 1 at the same side of the detecting position and the dyeing position. A rising arc plate 24 is fixedly connected at the detecting position and the dyeing position inside the supporting bottom cover 1 for moving the rotating member 17 upward. An outer tooth plate 25 with adjustable position is arranged on the unlocking position of the supporting bottom cover 1. A screw rod 26 is fixedly connected to the surface of the outer tooth plate 25. One end of the screw rod 26 is threadedly penetrates the supporting bottom cover 1 and extends to the outside thereof. A guide rod 27 is also fixedly connected to the surface of the outer tooth plate 25. The guide rod 27 is movably connected to the supporting bottom cover 1. An inner tooth plate 28 is fixedly connected to the fixed position of the supporting bottom cover 1. When the adjusting portion 703 moves to the position of the inner tooth plate 28, it rolls on the inner tooth plate 28 to rotate the rotating member 17, and drives the arc-shaped clamping plate 6 to move toward each other through the gear-moving member on the abutting portion 702.

[0044] The geared member includes an upper gear ring 29 and a lower gear ring 30. The upper gear ring 29 is connected to the surface of the abutment portion 702 through a positioning bearing, and the lower gear ring 30 is slidably connected to the surface of the abutment portion 702 through a connecting block. Tooth blocks 31 are provided on opposite sides of the upper gear ring 29 and the lower gear ring 30. The tooth blocks 31 on the upper gear ring 29 and the tooth blocks 31 of the lower gear ring 30 are arranged opposite to each other, and magnet sheets 32 are fixedly connected to the surfaces of the tooth blocks 31; an abutment spring 33 is fixedly connected to the surface of the lower gear ring 30, and one end of the abutment spring 33 is fixedly connected to the surface of the abutment portion 702.

[0045] In this embodiment, the upper gear ring 29 and the opposite side of the upper gear ring 29 are fixedly connected with a tooth block 31, the tooth block 31 is arranged at an angle, and a magnet sheet 32 ​​is also fixedly connected to the tooth block 31. When the rotating member 17 rotates, the lower gear ring 30 and the upper gear ring 29 rotate due to magnetic force, and the two arc-shaped clamping plates 6 on the placement plate 4 are moved toward each other through the transmission component, thereby fixing the culture dish.

[0046] Specifically, the transmission component includes a screw rod 34 and a transmission bevel gear 35. The screw rod 34 is movably connected to the placement plate 4. The arc-shaped clamping plate 6 is threadedly sleeved on the surface of the screw rod 34. The transmission bevel gear 35 is fixedly sleeved on the surface of the screw rod 34. The bottom of the placement plate 4 is also movably connected with an active bevel gear 36 and a pressure plate 37 through a connecting shaft. The active bevel gear 36 is meshed with the transmission bevel gear 35, and the opposite sides of the pressure plate 37 and the upper gear ring 29 are fixedly connected with extrusion teeth 38.

[0047] In this embodiment, the transmission component includes an active bevel gear 36 and a pressure plate 37 movably connected to the bottom of the placement plate 4 through a connecting shaft. A plurality of extrusion teeth 38 are evenly fixed in an array on the pressure plate 37. When the rotating member 17 moves upward, the upper gear ring 29 docks with the extrusion teeth 38 between the pressure plate 37. Therefore, when the rotating member 17 rotates, the active bevel gear 36 can drive the two transmission bevel gears 35 meshing with it to rotate through the connecting shaft, thereby realizing the rotation of the screw rod 34, and then the arc-shaped clamping plates 6 can move toward each other to fix the culture dish.

[0048] Specifically, an electromagnet 39 is fixedly connected to the position of the support frame 3 corresponding to the placement plate 4, and a brake member 40 is movably connected to the surface of the placement plate 4. A magnet block 41 is fixedly connected to the surface of the brake member 40. When dyeing is required, after the laser receiver 11 receives the optical signal from the laser transmitter 10, the electromagnet 39 is energized to separate the brake member 40 from the placement plate 4 by adsorbing the magnet block 41.

[0049] In this embodiment, when dyeing is not required, the electromagnet 39 remains energized, and the current thereon can cause the magnet block 41 to be subjected to a repulsive force, thereby causing it to drive the brake member 40 to squeeze the placement plate 4 to prevent the placement plate 4 from rotating. When dyeing is required, a reverse current is passed through the electromagnet 39, and the rotating member 17 is disengaged from the placement plate 4, so that the rotation can be achieved under the action of the rotating frame.

[0050] When in use, the culture dish containing the cell fluid is placed on the placement tray 4. Each placement tray 4 is provided with two arc-shaped clamping plates 6. The spacing between the two arc-shaped clamping plates 6 is wide enough to place a culture dish of conventional maximum size. The staining rack 2 is fixed to the support frame 3 by screws, and the pre-prepared stain is injected into it.

[0051] After the culture dish is placed on the placement tray 4, the driving motor 8 is started. Under the action of the driving motor 8, the rotating shaft 9 is driven to rotate, and the rotating shaft 9 drives the support frame 3 to rotate, so that the plurality of placement trays 4 thereon can be driven to rotate simultaneously. In this process, the electromagnet 39 is kept energized, and the current on the electromagnet 39 can cause the magnet block 41 to be subjected to a repulsive force, so that it can drive the brake member 40 to squeeze the placement tray 4 to prevent the placement tray 4 from rotating. A rotating frame is fixedly connected to the bottom of the placement tray 4, and a rotating member 17 is connected to the interior of the rotating frame. The rotating member 17 includes an intermediate portion 701, which is integrally formed in the middle. The abutment portion 702 at the top of the middle portion 701 and the adjusting portion 703 integrally formed at the bottom of the middle portion 701, under the action of the support frame 3, the bottom of the adjusting portion 703 will contact the surface of the rising arc plate 24, so that it will be squeezed and move upward under the action of the rising arc plate 24. At this time, the connecting spring 21 is stretched, and after the rotating member 17 rises to the highest point, the outer teeth 19 on the middle portion 701 are disengaged from the inner teeth 20 inside the circular tube portion 501, and one end of the connecting spring 21 is connected to the inside of the circular tube portion 501 through a limit bearing, which will not cause any influence when the rotating member 17 rotates.

[0052] When unlocking is not required, the outer tooth plate 25 will not contact the teeth on the adjusting portion 703 under the action of the screw 26, so that it will not rotate. Then the adjusting portion 703 contacts the inner tooth plate 28 and meshes with the teeth thereon. The adjusting portion 703 drives the intermediate portion 701 and the abutting portion 702 thereon to rotate. The abutting portion 702 is also movably connected with an upper tooth ring 29, and a plurality of extrusion teeth are evenly arrayed on the top of the upper tooth ring 29. A lower tooth ring 30 is also slidably sleeved on the abutting portion 702. Specifically, the lower tooth ring 30 is 0, the inner connecting block is connected to the inner side wall of the abutment portion 702, an abutment spring 33 is fixedly connected between the lower gear ring 30 and the abutment portion 702, the upper gear ring 29 and the opposite side of the upper gear ring 29 are fixedly connected with tooth blocks 31, the tooth blocks 31 are arranged obliquely, and a magnet sheet 32 ​​is fixedly connected to the tooth blocks 31. When the rotating member 17 rotates, the lower gear ring 30 and the upper gear ring 29 rotate due to magnetic force, and the two arc-shaped clamping plates 6 on the placement plate 4 move toward each other through the transmission component, so as to fix the culture dish.

[0053] The transmission component includes an active bevel gear 36 and a pressure plate 37 movably connected to the bottom of the placement plate 4 through a connecting shaft. A plurality of extrusion teeth 38 are evenly fixed on the pressure plate 37. When the rotating member 17 moves upward, the upper gear ring 29 docks with the extrusion teeth 38 between the pressure plate 37. Therefore, when the rotating member 17 rotates, the active bevel gear 36 drives the two transmission bevel gears 35 meshing with it to rotate through the connecting shaft, thereby realizing the rotation of the screw rod 34, and then the arc-shaped clamping plates 6 can move toward each other to fix the culture dish. Inclined surfaces are provided on both sides of the rising arc plate 24 to facilitate the rising and falling of the rotating member 17. When the rotating member 17 is out of contact with the rising arc plate 24, the rotating member 17 automatically resets downward under the action of the connecting spring 21, and the outer teeth 19 on the middle part 701 are again in contact and meshed with the inner teeth 20 inside the circular tube part 501, so as to limit the rotating member 17 and prevent shaking.

[0054] When one of the placement trays 4 moves to the dyeing position, the laser emitted by the laser transmitter 10 on the dye will be received by the laser receiver 11, and the received light signal will be transmitted to the processor. After processing by the processor, the driving motor 8 is powered off and self-locked through the controller, so that the support frame 3 stops rotating. At this time, the circular tube portion 501 on the rotating member 17 contacts the transmission member, and the teeth on the circular tube portion 501 can mesh with the teeth on the transmission member 7. Then the driving motor 12 is started, driving the threaded rod 13 and the transmission pulley 18 on its output shaft to rotate. The rotation of the threaded rod 13 can cause the piston 14 on it to move upward, so that the dye inside the dyeing frame 2 can be pushed out of the dyeing tube. At the same time, the transmission pulley 18 rotates the rotating member 17 through the belt, which can drive the circular tube portion 501 to rotate, and then the placement tray 4 can be rotated through the support portion 502 on the circular tube portion 501, and then the culture dish can be rotated, so that the dye can be evenly dripped into the culture dish.

[0055] The filling of the dye is timed by a timer. After the timing is over, the drive motor 12 is powered off and the drive motor 8 is restarted. Then the support frame 3 continues to rotate, and the electromagnet 39 is supplied with reverse current to repel the magnet block 41, so that the brake member 40 can fix the placement plate 4. Then the adjustment part 703 will contact the vibration-assisting plate 22 fixed inside the support bottom cover 1. The vibration-assisting plate 22 is provided with an inclined surface. Under the action of the inclined surface, the adjustment part 703 drives the entire rotating part 17 to move downward. At this time, the connecting spring 21 is compressed to the minimum. A plurality of baffles 23 are evenly arranged in an array on the adjustment part. At the same time, a plurality of vibration points are fixedly connected to the vibration-assisting plate 22. The baffles 23 contact the vibration points and vibrate. According to the density of the baffles 23 and the density of the vibration points, high-frequency and low-amplitude vibrations can be achieved, so that the rotating frame is vibrated through the rotating member 17, and then the placement plate 4 is rotated. The components of the cell fluid and the dye can be mixed, which is convenient for rapid dyeing.

[0056] Since dyeing may take a long time, the dyeing rack 2 can be removed after all the culture dishes are filled with dye. Under the action of the drive motor 12, the support frame 3 continues to rotate. After the brake member 40 is out of contact with the vibration-assisting plate 22, it will contact the inner tooth plate 28 again. Under the action of the inner tooth plate 28, the rotating member 17 rotates again to achieve the opposite movement of the arc-shaped clamping plate 6. This design makes it possible for the arc-shaped clamping plate 6 to loosen when the vibration-assisting plate 22 rotates the rotating member 17, resulting in unstable clamping or even offset. However, continuous rotation of the rotating member 17 may cause the arc-shaped clamping plate 6 to continue to pressurize the culture dish, causing the culture dish to rupture or even the support frame 3 to be unable to rotate. Therefore, the lower tooth ring 30 and the upper tooth ring 29 can move relative to each other.

[0057] As mentioned above, the upper toothed ring 29 is connected to the lower toothed ring 30 by the magnet sheet 32, and the upper toothed ring 29 is movably connected to the abutment portion 702, and the lower toothed ring 30 is slidably connected to the abutment portion 702. When the culture dish causes the arc-shaped clamping plate 6 to rotate for lunch, the extrusion force will be concentrated on the extrusion teeth 38 between the upper toothed ring 29 and the pressure plate 37, and the upper toothed ring 29 and the lower toothed ring 30 are connected by magnetic force. Therefore, when the upper toothed ring 29 cannot rotate and the rotating part 17 at the bottom continues to rotate, the lower toothed ring 30 is pressed and moves downward, and under the action of the abutment spring 33, it reciprocates up and down to realize the continuous rotation of the rotating part 17, and the arc-shaped clamping plate 6 will not move, thereby protecting the culture dish. This design is also conducive to clamping culture dishes of different sizes.

[0058] With the help of the vibration-assisting plate 22, the cells inside the cell fluid are quickly stained with the dye. After the staining is completed, it is detected by an electron microscope at the detection position. Finally, the outer tooth plate 25 moves toward the direction close to the inside of the supporting bottom cover 1 through the screw 26 to achieve the contact between the outer tooth plate 25 and the adjustment part 703. Under the action of the outer tooth plate 25, the adjustment part 703 is reversed, so that the arc-shaped clamping plate 6 can be moved away from each other through the rotating component, and the contact with the culture dish is broken, so that the culture dish can be disassembled.

[0059] Through the above scheme, the culture dish can be automatically fixed and vibrated, and dye can be added to the cell fluid, which is convenient for rapid detection of cells and shortening of dyeing time, thereby shortening the detection cycle.

[0060] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0061] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A cell detection device, comprising a support bottom cover (1) provided with a detection position and a staining position, wherein an electron microscope is fixedly mounted at the detection position of the support bottom cover (1), characterized in that: Also includes: A dyeing frame (2) detachably mounted at the dyeing position of the supporting bottom cover (1), wherein an extrusion component for pushing the dye out of a liquid outlet pipe on the dyeing frame (2) is arranged inside the dyeing frame (2); A rotatable support frame (3) is arranged on the support bottom cover (1), and a plurality of rotatable placement plates (4) for placing cell culture dishes are arranged in an array on the support frame (3). The bottom of each placement plate (4) is fixedly connected to a transmission frame (5), and the transmission frame (5) is arranged so that when the placement plate (4) is rotated to the staining position of the staining frame (2), the placement plate (4) can be rotated when the extrusion component pushes the staining agent out of the liquid outlet pipe on the staining frame (2); Two arc-shaped clamping plates (6) are arranged on the placement plate (4) for clamping the culture dish placed on the placement plate (4), and a transmission member (7) is arranged inside the transmission frame (5). The transmission member (7) is assembled so that after upward movement, the two arc-shaped clamping plates (6) can move towards or away from each other; after downward movement, the placement plate (4) can vibrate. The bottom of the support bottom cover (1) is fixedly connected to a driving motor (8), and the end of the output shaft of the driving motor (8) is fixedly connected to a rotating shaft (9). The rotating shaft (9) movably penetrates the support bottom cover (1) and is fixedly connected to the support frame (3). The bottom of the support frame (3) is movably connected to the inner side wall of the support bottom cover (1) via a connecting bearing. The staining frame (2) is fixedly connected to the support frame (3) by screws, a laser emitter (10) is fixedly connected to the staining frame (2), and laser receivers (11) are fixedly connected to positions on the support frame (3) corresponding to the placement plates (4). When staining is required, after the laser receiver (11) receives the optical signal from the laser emitter (10), the controller turns off the power to the drive motor (8), and the extrusion component pushes the dye out of the liquid outlet pipe on the staining frame (2), while the transmission frame (5) drives the placement plates (4) to rotate, so that the dye is in uniform contact with the cells inside the culture dish; The extrusion component comprises a driving motor (12) and a threaded rod (13); the driving motor (12) is fixedly connected to the bottom of the dyeing frame (2); an output shaft thereof movably penetrates the dyeing frame (2) and is fixedly connected to one end of the threaded rod (13); a piston (14) is threadedly sleeved on the surface of the threaded rod (13); a guide block (15) is fixedly connected to the surface of the piston (14); the surface of the guide block (15) is movably connected to the inner side wall of the dyeing frame (2); a support plate (16) is fixedly connected to the surface of the dyeing frame (2); a rotating member (17) is fixedly connected to the bottom of the support plate (16); a transmission pulley (18) is fixedly sleeved on the output shaft of the driving motor (12); and the rotating member (17) is connected to the transmission pulley (18) via a belt drive; The transmission frame (5) comprises a circular tube portion (501) and a support portion (502), wherein the support portion (502) is fixedly connected to the top of the circular tube portion (501), the top of the support portion (502) is fixedly connected to the surface of a placement plate (4), the surface of the circular tube portion (501) is fixedly connected with teeth meshing with a rotating member (17), and the placement plate (4) is connected to the support frame (3) via a fixed bearing.

2. A cell detection device according to claim 1, characterized in that: The transmission member (7) comprises a middle portion (701), an abutting portion (702) and an adjusting portion (703), wherein the abutting portion (702) is integrally formed at the top of the middle portion (701), and the adjusting portion (703) is integrally formed at the bottom of the middle portion (701); a surface of the middle portion (701) is uniformly arrayed with a plurality of external teeth (19); an interior of the circular tube portion (501) is uniformly arrayed with a plurality of internal teeth (20); and a surface of the external teeth (19) is movably connected to a surface of the internal teeth (20); The abutment portion (702) is provided with a geared member, and after the rotating member (17) moves upward, the outer teeth (19) and the inner teeth (20) are disengaged, and the adjusting portion (703) drives the rotating member (17) to rotate, so that the geared member on the abutment portion (702) causes the arc-shaped clamping plates (6) to move away from or towards each other through the transmission component; A connection spring (21) is fixedly connected to the surface of the abutment portion (702), and one end of the connection spring (21) is connected to the interior of the circular tube portion (501) via a limit bearing.

3. A cell detection device according to claim 2, characterized in that: A vibration-assisting plate (22) is fixedly connected to the inside of the support bottom cover (1) on the same side of the detection position and the dyeing position, and both sides of the vibration-assisting plate (22) are provided with inclined surfaces facing the bottom of the support bottom cover (1), and a plurality of vibration points are evenly arrayed on the bottom surface of the vibration-assisting plate (22), and a plurality of retaining bars (23) are fixedly evenly arrayed on the top surface of the adjustment portion (703).

4. A cell detection device according to claim 2, characterized in that: An unlocking position and a fixing position are sequentially arranged inside the support bottom cover (1) at the same side of the detection position and the dyeing position. A rising arc plate (24) is fixedly connected to the detection position and the dyeing position inside the support bottom cover (1) for moving the rotating member (17) upward. An outer tooth plate (25) with adjustable position is arranged on the unlocking position of the support bottom cover (1). A screw rod (26) is fixedly connected to the surface of the outer tooth plate (25). One end of the screw rod (26) is threadedly passed through the support bottom cover (1) and extends to the outside thereof. A guide rod (27) is also fixedly connected to the surface of the outer tooth plate (25). The guide rod (27) is movably connected to the support bottom cover (1). An inner tooth plate (28) is fixedly connected to the fixed position of the support bottom cover (1); when the adjustment portion (703) moves to the position of the inner tooth plate (28), the adjustment portion (703) rolls on the inner tooth plate (28) to rotate the rotating member (17), and the transmission member drives the arc-shaped clamping plate (6) to move toward each other through the toothed member on the abutting portion (702).

5. A cell detection device according to claim 4, characterized in that: The geared member comprises an upper gear ring (29) and a lower gear ring (30); the upper gear ring (29) is connected to the surface of the abutment portion (702) via a positioning bearing; the lower gear ring (30) is slidably connected to the surface of the abutment portion (702) via a connecting block; tooth blocks (31) are arranged on opposite sides of the upper gear ring (29) and the lower gear ring (30); the tooth blocks (31) on the upper gear ring (29) and the tooth blocks (31) on the lower gear ring (30) are arranged opposite to each other, and magnet sheets (32) are fixedly connected to the surfaces of the tooth blocks (31); An abutment spring (33) is fixedly connected to the surface of the lower gear ring (30), and one end of the abutment spring (33) is fixedly connected to the surface of the abutment portion (702).

6. A cell detection device according to claim 5, characterized in that: The transmission component comprises a screw rod (34) and a transmission bevel gear (35), the screw rod (34) is movably connected to the placement plate (4), the arc-shaped clamping plate (6) is threadedly sleeved on the surface of the screw rod (34), the transmission bevel gear (35) is fixedly sleeved on the surface of the screw rod (34), and the bottom of the placement plate (4) is also movably connected to a driving bevel gear (36) and a pressure plate (37) via a connecting shaft, the driving bevel gear (36) is meshed with the transmission bevel gear (35), and the pressure plate (37) and the upper gear ring (29) are fixedly connected to extrusion teeth (38) on opposite sides.

7. A cell detection device according to any one of claims 1 to 6, characterized in that: An electromagnet (39) is fixedly connected to a position on the support frame (3) corresponding to the placement plate (4), a brake component (40) is movably connected to the surface of the placement plate (4), and a magnet block (41) is fixedly connected to the surface of the brake component (40). When dyeing is required, after the laser receiver (11) receives the optical signal from the laser transmitter (10), the electromagnet (39) adsorbs the magnet block (41) to separate the brake component (40) from the placement plate (4).

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