Cosmetic compositions and methods of use thereof

Abstract

Compositions comprising an extract from Hibiscus and an extract from damaged plant parts of Moringa, and methods of stimulating collagen synthesis in skin cells are described herein. Compositions comprising an extract from Hibiscus, an extract from damaged plant parts of Moringa, and a complex of actives comprising at least one oligopeptide, at least one extract from Laminaria, and whey protein are also described herein.

Description

Technical Field

[0001] The present disclosure is in the field of cosmetic compositions for treating aged skin and has particular efficacy in stimulating collagen synthesis in skin cells. Background Art

[0002] Collagen is one of the main structural proteins in the skin. It can exist in fibrous or non-fibrous forms. The fibrous form is the most common and includes collagen subtypes I, II, III, V and XI. Types I, IV and V are most commonly associated with skin and dermal tissue. The collagen found in the skin usually decreases with age and causes sagging, fine lines and wrinkles on the skin. Any active ingredient that induces skin cells to increase collagen synthesis is ideal because it improves the side effects of collagen deficiency in skin cells that cause fine lines, wrinkles and skin sagging.

[0003] It has been unexpectedly found that the combination of an extract obtained from Hibiscus and an extract obtained from Moringa synergistically improves the stimulation of collagen synthesis in skin cells. And it has also been unexpectedly found that the complex of actives further synergistically improves the efficacy of the combination of an extract obtained from Hibiscus and an extract obtained from Moringa on stimulating collagen synthesis in skin cells. Summary of the invention

[0004] The present disclosure relates to topical compositions comprising an extract from Hibiscus and an extract from Moringa.

[0005] The present disclosure also relates to a topical composition comprising an extract from Hibiscus, an extract from Moringa, and a complex of actives comprising at least one oligopeptide, at least one extract from Laminaria, and whey protein.

[0006] The present disclosure also relates to methods of using the topical composition comprising an extract from Hibiscus and an extract from Moringa.

[0007] The present disclosure also relates to methods of using a topical composition comprising a topical composition comprising an extract from Hibiscus, an extract from Moringa, and a complex of actives comprising at least one oligopeptide, at least one extract from Laminaria, and whey protein. DETAILED DESCRIPTION

[0008] definition

[0009] As used herein, "Hibiscus" refers to all synonyms: "Abelmoschus mutabilis," "Abelmoschus venustus," "Hibiscus immutabilis," "Hibiscus javanicus," "Hibiscus mutabilis," and "Ketmia mutabilis."

[0010] "Ethanol extract" refers to an extract that has undergone an extraction step from the plant in ethanol or a water / ethanol solution. It does not refer to an extract that has been subjected to an extraction step in another solvent that is subsequently evaporated and the precipitate is redissolved in ethanol or a water / ethanol solution.

[0011] "Hydroglycolic extract" means that the extraction step from the plant is carried out in a water / butylene glycol solution. It does not refer to an extract carried out by extraction in another solvent which is subsequently evaporated, the precipitate being redissolved in a water / butylene glycol solution.

[0012] As used herein, "and / or" means "and" or "or". For example, "A and / or B" means "A, or B, or both A and B". Therefore, within the meaning of the present disclosure, "white flowers and / or light pink flowers" means only white flowers, or only light pink flowers, or a mixture of white flowers and light pink flowers, and the ratio of white flowers to light pink flowers is irrelevant.

[0013] A. Composition

[0014] In some embodiments of the present disclosure, the composition may be in liquid, semisolid or solid form, and may be an emulsion, solution, suspension or anhydrous form.

[0015] In some embodiments, the compositions of the present disclosure may include an extract from Hibiscus and an extract from Moringa. In some aspects, the compositions of the present disclosure may further include a complex of actives including at least one oligopeptide, at least one extract from Laminaria, and whey protein.

[0016] 1. Extract from Hibiscus :

[0017] The composition may comprise at least one extract obtained by extracting a plant part from a plant of the genus Hibiscus. The extract from Hibiscus may be present in an amount ranging from about 0.001-5%, preferably about 0.005-3%, more preferably about 0.01-2%, and most preferably about 0.1-1% by weight of the total composition.

[0018] Hibiscus is a genus of flowering plants, including both annual and perennial herbs, as well as woody shrubs and small trees. The flower color of certain species of the genus Hibiscus (e.g., Hibiscus mutabilis and Hibiscus tiliaceus) varies with the age of the flower. Preferred Hibiscus species are plants whose flower color varies with the age of the flower. A more preferred Hibiscus species is Hibiscus mutabilis.

[0019] Plant parts that can be used include stems, leaves, roots, buds, seeds, twigs, flowers, bark, etc. Preferred plant parts are leaves, twigs, bark and flowers. The most preferred plant part is flower.

[0020] Hibiscus flowers unfold their corolla in the morning when the flowers are tinged with a creamy white color. Over the course of the day, the petals gradually turn pink, and at the end of flowering they are an intense pink. Thus, white or light pink Hibiscus flowers are considered juvenile flowers, while red Hibiscus flowers are not considered juvenile flowers. Preferred Hibiscus flowers are juvenile flowers (i.e., white flowers and / or light pink flowers).

[0021] Suitable extractants include water, alkanes, ethers, aromatic solvents, ketones, and the like. Water alone, water / solvent mixtures, or solvent alone are preferred, wherein the solvent comprises a monohydric alcohol, a dihydric alcohol, or a polyhydric alcohol, such as ethanol, propanol, isopropanol, butanol, methanol. The extractant may be combined with the plant part in any suitable ratio, including a solvent / water ratio of 1:1-10 to 1-10:1, respectively. More preferably, the hibiscus extract is a hydrodiol extract of hibiscus flowers.

[0022] In some aspects, the extract from Hibiscus according to the present disclosure can be made from young Hibiscus flowers. In some preferred aspects, the extract from Hibiscus according to the present disclosure can be made from young Hibiscus flowers that do not show signs of aging. In some alternative preferred aspects, the extract from Hibiscus according to the present disclosure can be made from young Hibiscus flowers that show minimal concentrations of the oxidation marker Cy-3-S.

[0023] In some preferred aspects, the extract from Hibiscus mutabilis according to the present disclosure can be obtained from white flowers and / or light pink flowers of Hibiscus mutabilis. In some more preferred aspects, the extract from Hibiscus mutabilis according to the present disclosure can contain at least 2 ppm of N-(1-deoxy-1-fructosyl)-proline (a glycosylated amino acid with a molar mass of 277 Da) and / or at least 145 ppm of cyperoside epoxide (a glycoside with a molar mass of 291 Da).

[0024] In contrast, the hydrodiol extract of red Hibiscus mutabilis flower contained little or no of these two molecules (less than 2 ppm of N-(1-deoxy-1-fructosyl)-proline and less than 145 ppm of cyperoside epoxide).

[0025] In some more preferred aspects, the extract of Hibiscus mutabilis according to the present disclosure contains little or no mature marker molecules, i.e., citric acid and another organic acid with a structure close to citric acid. In some most preferred aspects, the extract of Hibiscus mutabilis contains less than 150 ppm of citric acid with a molar mass of 192 Da, and / or less than 60 ppm of isomers of citric acid with a molar mass of 192 Da.

[0026] In contrast, the hydrodiol extract of red Hibiscus mutabilis flowers contained more than 150 ppm of citric acid and more than 60 ppm of an isomer of citric acid having a molar mass of 192 Da.

[0027] In some particularly preferred aspects, the extract from Hibiscus mutabilis according to the present disclosure may have at least one feature selected from the following: the extract from Hibiscus mutabilis flower contains at least 50% carbohydrates by dry weight of the extract; the extract from Hibiscus mutabilis flower contains minerals and / or proteins, preferably less than 20% minerals and / or less than 20% proteins, the percentages being given by dry weight of the extract; at least 30% by dry weight of the carbohydrates present in the extract from Hibiscus mutabilis flower are oligosaccharides and polysaccharides with a molar mass between 360-1,620 Da; and the proteins present in the extract from Hibiscus mutabilis flower are peptides with a molar mass below 2,000 Da. In some most preferred aspects, the extract from Hibiscus mutabilis flower may have all of the above features in this paragraph.

[0028] The most preferred extract from Hibiscus mutabilis according to the present disclosure, which can be obtained from Hibiscus mutabilis white flowers and / or light pink flowers, has all of the following characteristics: comprises at least 2 ppm of N-(1-deoxy-1-fructosyl)-proline and / or at least 145 ppm of cyperoside epoxide; comprises less than 150 ppm of citric acid with a molar mass of 192 Da and / or less than 60 ppm of isomers of citric acid with a molar mass of 192 Da; comprises at least 50% of carbohydrates by dry weight of the extract; comprises less than 20% of minerals and / or less than 20% of proteins, the percentages being given by dry weight of the extract; at least 30% by dry weight of the carbohydrates present in the extract from Hibiscus mutabilis are oligosaccharides and polysaccharides with a molar mass between 360-1,620 Da; and the proteins present in the extract from Hibiscus mutabilis are peptides with a molar mass below 2,000 Da.

[0029] A suitable extract from Hibiscus mutabilis may be obtained by a process comprising the steps of dissolving Hibiscus mutabilis flower powder in a water / butylene glycol mixture; separating the soluble and insoluble phases to recover the soluble phase; filtering; and sterile filtering.

[0030] Examples of suitable extracts from Hibiscus mutabilis are available under the trade name Purchased from SILAB.

[0031] 2. Extract from Moringa :

[0032] The composition may comprise at least one extract obtained by extracting damaged plant parts from a plant of the genus Moringa. The extract may be present in an amount ranging from about 0.001-5%, preferably about 0.005-3%, more preferably about 0.01-2%, and most preferably about 0.1-1% by weight of the total composition.

[0033] Plants from this genus grow commonly in Africa and India. The Moringaceae family is a genus of flowering plants, ranging in size from large trees to small flowering plants. There are 13 species. Species drouhardii, hildebrandtii, ovalifolia and stenopetala are large trees with large water-storing trunks ("bottle trees") and small radial and symmetrical flowers. Species concanensis, oleifera and pygmaea are slender trees with tuberous juvenile stages and gray or pink flowers. Species arborea, borziana, longituba, pygmaea, rivae, ruspoliana are trees, shrubs or herbaceous forms. Moringa is especially a fast-growing tree, commonly known as the "drumstick tree". Moringaceae leaves are said to contain large amounts of vitamins, polyphenols and four unique sugar-modified aromatic glycosides. Isothiocyanates from the Moringaceae family (or Moringa isothiocyanates ("MIC")) are said to have many health benefits. Typically, isothiocyanates are formed when the enzyme myrosinase (also known as thioglucosyl glycohydrolase) cleaves the sulfur-linked glucose in the precursor glucosinolate. Moringa extracts with higher concentrations of MIC are most desired. Such extracts can be prepared by damaging plant parts to cause an increase in myrosinase content, which in turn promotes the conversion of Moringa glucosinolates ("MGLs") to MICs.

[0034] Moringa extracts that can be used in the compositions of the present invention are further described in Phytochemistry, Vol. 103 (2014), pp. 114-122; and U.S. Provisional Patent Application Serial No. 61 / 898795, filed on November 1, 2013; U.S. Provisional Patent Application Serial No. 62 / 032496, filed on August 1, 2014; and U.S. Serial No. 14 / 683730, a continuation-in-part application of PCT / US2014 / 0063178, filed on October 30, 2014, all of which are incorporated herein by reference in their entirety.

[0035] Damage to plant parts may occur by processing steps such as pressing, slicing, pulverizing, crushing, blending or grinding. Damage (and myrosinase production) may also be induced by subjecting plant parts to solutions containing water. The solution may be all water, or a mixture of various solvents (e.g., ethanol, propanol, isopropanol, butanediol, propylene glycol, pentanediol, etc.) and water. Preferably, such damage occurs at a temperature below 100° C., and without otherwise exposing the plant parts to extreme weather conditions, such as low temperatures or severe drying conditions. The conditions that induce plant damage should occur for a period of time sufficient to activate myrosinase in an amount sufficient to cause an increase in the production of MIC in the damaged plant parts.

[0036] Plant parts that can be used include stems, leaves, roots, buds, seeds, twigs, flowers, bark, etc. Most preferably, the damaged plant parts are from seeds, buds or leaves. Preferably, the plant parts are fresh, that is, they have not been dried or frozen.

[0037] After the plant parts are damaged, they can be extracted or dried. Suitable extractants can be water or a mixture of solvents as described above. The damaged plant parts can be extracted immediately or after drying.

[0038] Suitable extractants include water, alkanes, ethers, aromatic solvents, ketones, etc. Water alone, water / solvent mixtures, or solvent alone are preferred, wherein the solvent comprises a monohydric alcohol, a dihydric alcohol, or a polyhydric alcohol, such as ethanol, propanol, isopropanol, butanol, methanol. The extractant can be combined with the plant part in any suitable ratio, including a solvent / water ratio of 1:1-10 to 1-10:1, respectively.

[0039] Preferably the concentration of MIC in the extract should be at least 0.5-10% MIC per gram of plant material, preferably 0.75-4%, more preferably 0.8-5%. Harsh temperatures or drying of plant parts will cause rapid degradation of the MIC present in the plant parts and the resulting extract.

[0040] Most preferably, the Moringa seed extract contains about 0.5-3.0% MIC, more preferably 0.75-2.5% MIC, and most preferably about 0.8-1.5% MIC. The extract can be obtained under the trade name Available from Nutrasorb LLC, it is a mixture of Moringa seed extract and isoceteth-20 in a ratio of about 7.5:92.5, respectively, and contains about 1% MIC. When tested for stability at 25°C and 37°C for 30 days, the best embodiment extract has a MIC content of greater than 65%, preferably greater than 70%.

[0041] Moringa seed extracts that may be used in the compositions of the present invention are further described in U.S. Pat. No. 9,687,439, which is incorporated herein by reference in its entirety, wherein Moringa seed extracts themselves were found to have no activity in stimulating collagen production in normal human dermal fibroblasts ("NHDF"), and in fact, in most cases, caused a decrease in collagen synthesis.

[0042] 3. Oligopeptides :

[0043] The composition may comprise at least one oligopeptide in an amount ranging from about 0.000001-5%, preferably about 0.00001-2%, more preferably about 0.0005-1% by weight of the total composition.

[0044] Suitable oligopeptides are those having about 2-20, preferably about 4-10, or most preferably 5-6 amino acids. The peptides may be substituted by acyl groups (e.g., acetyl, palmitoyl, etc.). Examples of suitable oligopeptides include, but are not limited to, dipeptides, tripeptides, pentapeptides, hexapeptides, heptapeptides, etc. Suitable acyl groups include acetyl, palmitoyl, or myristoyl. Further specific examples include hexapeptides 1-60, wherein the range includes each integer between 1-60, acetylated, palmitoyl, or myristoyl hexapeptides, such as acetyl hexapeptides 1, 7, 8, 19, 20, 22, 24, 30, 31, 37, 38, 39, or 40, particularly preferably acetyl hexapeptide-8 obtained by acetylation of hexapeptide-8 (a synthetic peptide containing arginine, glutamic acid, glutamine, and methionine). Acetyl hexapeptide-8 can be obtained under the trade name Purchased from Lipotec

[0045] SA, which is a solution of about 0.05 parts of acetyl hexapeptide-8, 93.35 parts of water, and the remainder being a preservative.

[0046] Pentapeptides that may be acetylated, palmitoylated or myristoylated are also suitable. Examples of such pentapeptides include pentapeptide 1-50, including every integer therebetween. Palmitoyl pentapeptide-5 is particularly preferred.

[0047] Particularly preferred are oligopeptides with the INCI names acetyl-hexapeptide-8, palmitoyl oligopeptide, tripeptide-32, tetrapeptide-26, palmitoyl hexapeptide-12, oligopeptide-10, oligopeptide-5, oligopeptide-3, pentapeptide-3, tetrapeptide-51 amide, heptapeptide, palmitoyl pentapeptide-5 or combinations thereof. These peptides are defined by the International Nomenclature of Cosmetic Ingredients (INCI) and are terms known in the art.

[0048] 4. Extract from Laminaria japonica :

[0049] The composition may include at least one extract from Laminaria. Laminaria is a genus containing 30+ species of brown algae, commonly known as giant algae. Such extracts from Laminaria include those of the species abyssalis, agardhii, appressirhiza, brasiliensis, brongardiana, bulbosa, bullata, complanata, digitata, ephemera, farlowii, groenlandica, hyperborea, inclinitorhiza, multiplicata, nigripes, ochroleuca, pallida, platymeris, rodriguezi, ruprechtii, sachalinensis, setchellii, sinclairii, solidungula or yezoensis. Preferably, the extract from Laminaria is also a SIRT3 activator. Preferably the extract is from Laminaria digitata, and more particularly the extract has a laminarin content and / or mannitol content in the range of 0.5-3 wt%, or about 0.75-2.5 wt%, or most preferably about 1 wt% or more, preferably about 2%. An example of a suitable extract of Laminaria digitata is available from Barnet Products under the trade name Mitostime Di, which is a mixture of 91 parts water, 8 parts extract of Laminaria digitata and 1 part preservative. Preferably the extract of Laminaria digitata is obtained by aqueous extraction, and leaching of freeze-dried seaweed and sterilization microfiltration followed by reverse osmosis to concentrate the active molecules.

[0050] In a preferred embodiment of the present invention, the extract may be present in the composition in an amount ranging from 0.0001-5%, preferably about 0.001-2.5%, more preferably about 0.01-1%.

[0051] 5. Whey protein :

[0052] The composition may comprise whey protein in an amount ranging from 0.01-5%, preferably about 0.05-3%, more preferably about 0.1-2% by weight of the total composition.

[0053] Whey protein is a polypeptide obtained from the fluid part of milk after separation from the curd. Whey protein may be hydrolyzed. Most preferred is the whey protein sold by Glanbia Foods under the trade name Whey protein NXP.

[0054] 6. Active ingredient complex :

[0055] In one embodiment, the oligopeptides, kelp extract and whey protein can be supplied to the composition in the form of a pre-blend, which can then be formulated into the final product. In this case, a ratio of about 2-20 parts of oligopeptides, 1-10 parts of kelp extract and 0.1-5 parts of whey protein is suitable. Most preferably, a ratio of 10 parts of acetyl hexapeptide-8, 5 parts of palm kelp extract and 1 part of whey protein.

[0056] The compositions of the present invention may further "comprise" other ingredients, including but not limited to those described herein.

[0057] 7. Other ingredients :

[0058] Topical compositions may contain the following ingredients:

[0059] Oil

[0060] Suitable oils include silicones, esters, vegetable oils, synthetic oils, including but not limited to those described herein. The oil can be volatile or non-volatile, and is preferably in the form of a pourable liquid at room temperature. If present, the oil can be in the range of about 0.5-85% by weight of the total composition, preferably about 1-75% by weight, and more preferably about 5-65% by weight.

[0061] Cyclic and linear volatile silicones are available from a variety of commercial sources, including Dow Chemical Corporation and Momentive (formerly General Electric Silicones). Dow Chemical linear volatile silicones are sold under the trade names Dowsil and Xiameter 244, 245, 344 and 200 fluids. These fluids include hexamethyldisiloxane (viscosity 0.65 centistokes (abbreviated as cst)), octamethyltrisiloxane (1.0 cst), decamethyltetrasiloxane (1.5 cst), dodecamethylpentasiloxane (2 cst) and mixtures thereof, wherein all viscosity measurements are made at 25°C.

[0062] Suitable branched volatile silicones include alkyl trimethicone, such as methyl trimethicone, which is a branched volatile silicone having the general formula:

[0063]

[0064] Methyl trimethicone is commercially available from Shin-Etsu Silicones under the trade name TMF-1.5 and has a viscosity of 1.5 centistokes at 25°C.

[0065] Various straight or branched chain alkanes having 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 carbon atoms (more preferably 8-16 carbon atoms) are also suitable. Suitable hydrocarbons include pentane, hexane, heptane, decane, dodecane, tetradecane, tridecane and C 8-20 Isoparaffin. Suitable C 12 Isoparaffins are manufactured by Permethyl Corporation under the trade name Permethyl 99A. Various commercially available C 16 Isoparaffins such as isohexadecane (trade name Permethyl R) are also suitable.

[0066] The ester formed by the reaction of carboxylic acid and alcohol is also suitable.Alcohol and carboxylic acid can all have a fatty (C6-30) chain.Examples include hexyl laurate, butyl isostearate, hexadecyl isostearate, hexadecyl palmitate, isostearyl neopentanoate, stearyl enanthate, isostearyl isononanoate, stearyl lactate, stearyl octanoate, stearyl stearate, isononyl isononanoate etc.

[0067] Esters can also be in dimer or trimer form. Examples of such esters include diisostearyl malate, neopentyl glycol dioctanoate, dibutyl sebacate, dicetostearyl dilinoleate dimer, dicetyl adipate, diisocetyl adipate, diisononyl adipate, diisostearyl dilinoleate dimer, diisostearyl fumarate, diisostearyl malate, dioctyl malate, and the like.

[0068] Examples of other types of esters include those derived from arachidonic acid, citric acid or behenic acid, such as triarachidin, tributyl citrate, triisostearyl citrate, tri-C citrate, and tri-C esters. 12-13 Alkyl esters, tricaprylin, trioctyl citrate, tridecyl behenate, trioctyldodecyl citrate, tridecyl behenate; or tridecyl coconut oil, tridecyl isononanoate, etc.

[0069] Synthetic or naturally occurring fatty acid glycerides or triglycerides are also suitable for use in the compositions. Both plant and animal sources may be used. Examples of such oils include castor oil, lanolin oil, C 10-18 Triglycerides, caprylic / capric / triglycerides, sweet almond oil, almond oil, sesame oil, camelina oil, tamanu seed oil, coconut oil, corn oil, cottonseed oil, linseed oil, ink oil, olive oil, palm oil, ice grass fat, rapeseed oil, soybean oil, grapeseed oil, sunflower seed oil, walnut oil, etc.

[0070] Synthetic or semi-synthetic glycerides are also suitable, such as fatty acid mono-, di- and triglycerides, which are natural fats or oils that have been modified, such as mono-, di- or triesters of polyols (such as glycerol). 12-22 ) fatty carboxylic acid reacted with one or more repeating glyceryl groups. Stearic acid glyceryl, diisostearate diglyceryl, polyglyceryl-3 isostearate, polyglyceryl-4 isostearate, polyglyceryl-6 ricinoleate, dioleic acid glyceryl, distearic acid glyceryl, tetraisostearic acid glyceryl, tricaprylic acid glyceryl, distearic acid diglyceryl, linoleic acid glyceryl, myristic acid glyceryl, isostearic acid glyceryl, PEG castor oil, PEG oleic acid glyceryl, PEG stearic acid glyceryl, PEG tallow acid glyceryl, and the like.

[0071] Non-volatile silicones (both water soluble and water insoluble) are also suitable for use in the composition. Such silicones preferably have a viscosity at 25°C in the range of from about greater than 5 to 800,000 cSt, preferably 20-200,000 cSt. Suitable water-insoluble silicones include amine-functional silicones such as aminodimethicone. Examples include dimethicone, phenyl dimethicone, diphenyl dimethicone, phenyl trimethicone, or trimethylsiloxyphenyl dimethicone. Other examples include alkyl dimethicones such as cetyl dimethicone, stearyl dimethicone, behenyl dimethicone, and the like.

[0072] Surfactants

[0073] The composition may contain one or more surfactants, especially if in the form of an emulsion. However, if the composition is also anhydrous, such surfactants may be used and will help disperse polar ingredients, such as pigments. Such surfactants may be silicone-based or organic. The surfactant will help form a stable emulsion in the form of water-in-oil or oil-in-water. If present, the surfactant may range from about 0.001-30% by weight of the total composition, preferably about 0.005-25% by weight, and more preferably about 0.1-20% by weight.

[0074] Silicone surfactants may be generally referred to as dimethicone copolyols or alkyl dimethicone copolyols. In some cases, the number of ethylene oxide or propylene oxide repeating units in the polymer is also specific, such as dimethicone copolyol, also known as PEG-15 / PPG-10 dimethicone, which refers to dimethicone with substituents containing 15 ethylene glycol units and 10 propylene glycol units on the siloxane backbone. One or more methyl groups in the above general structure may also be substituted with longer chain alkyl groups (e.g., ethyl, propyl, butyl, etc.) or ethers (e.g., methyl ether, ethyl ether, propyl ether, butyl ether, etc.).

[0075] Examples of silicone surfactants are those sold by Dow Silicones under the trade names Dowsil 3225C Formulation Aid, with the CTFA name Cyclotetrasiloxane (and) Cyclopentasiloxane (and) PEG / PPG-18 Dimethicone; or 5225C Formulation Aid, with the CTFA name Cyclopentasiloxane (and) PEG / PPG-18 / 18 Dimethicone; or Dowsil 190 Surfactant, with the CTFA name PEG / PPG-18 / 18 Dimethicone; or Dowsil 193 Fluid, Dowsil 5200, with the CTFA name Lauryl PEG / PPG-18 / 18 Methicone; or Abil EM 90 sold by Goldschmidt, with the CTFA name Cetyl PEG / PPG-14 / 14 Dimethicone; or Abil EM 90 sold by Goldschmidt 97, CTFA name Bis-Cetyl PEG / PPG-14 / 14 Dimethicone; or Abil WE 09, CTFA name Cetyl PEG / PPG-10 / 1 Dimethicone in a mixture also containing Polyglyceryl-4 Isostearate and Hexyl Laurate; or KF-6011 sold by Shin-Etsu Silicones, CTFA name PEG-11 Methyl Ether Dimethicone; KF-6012 sold by Shin-Etsu Silicones, CTFA name PEG / PPG-20 / 22 Butyl Ether Dimethicone; or KF-6013 sold by Shin-Etsu Silicones, CTFA name PEG-9 Dimethicone; or KF-6015 sold by Shin-Etsu Silicones, CTFA name PEG-3 Dimethicone; or KF-6016 sold by Shin-Etsu Silicones, CTFA name PEG-4 Dimethicone. KF-6016 sold by Shin-Etsu Silicones under the CTFA name PEG-9 Methyl Ether Dimethicone; or KF-6017 sold by Shin-Etsu Silicones under the CTFA name PEG-10 Dimethicone; or KF-6038 sold by Shin-Etsu Silicones under the CTFA name Lauryl PEG-9 Polydimethylsiloxyethyl Dimethicone.

[0076] Also suitable are various types of cross-linked silicone surfactants, generally referred to as emulsifying elastomers, which contain at least one hydrophilic portion, such as a polyoxyalkylenated group. Polyoxyalkylene silicone elastomers that can be used in at least one embodiment of the present disclosure include those sold by Shin-Etsu Silicones under the names KSG-21, KSG-20, KSG-30, KSG-31, KSG-32, KSG-33; KSG-210, which is a dimethicone / PEG-10 / 15 crosspolymer dispersed in dimethicone; KSG-310, which is a PEG-15 lauryl dimethicone crosspolymer; KSG-320, which is a PEG-15 lauryl dimethicone crosspolymer dispersed in isododecane; KSG-330 (the former dispersed in triethylhexanoin), KSG-340 (which is a mixture of PEG-10 lauryl dimethicone crosspolymer and PEG-15 lauryl dimethicone crosspolymer).

[0077] Polyglycerolated silicone elastomers are also suitable, such as those disclosed in PCT / WO 2004 / 024798, which is hereby incorporated by reference in its entirety. Such elastomers include Shin-Etsu's KSG series, such as KSG-710, which is a dimethicone / polyglyceryl-3 crosspolymer dispersed in dimethicone; or lauryl dimethicone / polyglyceryl-3 crosspolymer dispersed in various solvents (e.g., isododecane, dimethicone, triethylhexanoin), sold by Shin-Etsu under the trade names KSG-810, KSG-820, KSG-830 or KSG-840. Silicones sold by Dow Silicones under the trade names 9010 and DC9011 are also suitable.

[0078] The composition may include one or more nonionic organic surfactants. Suitable nonionic surfactants include alkoxylated alcohols, or ethers formed by reacting an alcohol with an alkylene oxide (usually ethylene oxide or propylene oxide). Preferably, the alcohol is a fatty alcohol having 6-30 carbon atoms. Examples of such ingredients include Steareth 2-100, which is formed by reacting stearyl alcohol with ethylene oxide, and the number of ethylene oxide units is in the range of 2-100; Beheneth 5-30, which is formed by reacting behenyl alcohol with ethylene oxide, wherein the number of ethylene oxide repeating units is 5-30; Ceteareth 2-100, which is formed by reacting a mixture of cetyl alcohol and stearyl alcohol with ethylene oxide, wherein the number of ethylene oxide repeating units in the molecule is 2-100; Ceteth 1-45, which is formed by reacting cetyl alcohol with ethylene oxide, and the number of ethylene oxide repeating units is 1-45, etc. The description of all units includes all integers between the ranges.

[0079] Other alkoxylated alcohols are formed by the reaction of fatty acids and mono-, di- or polyols with alkylene oxides. 6-30 The reaction products of fatty carboxylic acids and polyols (which are monosaccharides such as glucose, galactose, methyl glucose, etc.) with alkoxylated alcohols. Examples include polymeric alkylene glycols reacted with glycerol fatty acid esters, such as PEG olein, PEG stearin, or PEG polyhydroxyalkanoates, such as PEG dipolyhydroxystearate, wherein the number of ethylene glycol repeating units is in the range of 3-1000.

[0080] Other suitable nonionic surfactants include alkoxylated sorbitan and alkoxylated sorbitan derivatives. For example, alkoxylation (particularly ethoxylation) of sorbitan provides polyalkoxylated sorbitan derivatives. Esterification of polyalkoxylated sorbitan provides sorbitan esters, such as polysorbates. For example, polyalkoxylated sorbitan can be esterified with C6-30 (preferably C12-22) fatty acids. Examples of such ingredients include polysorbate 20-85, sorbitan oleate, sorbitan sesquioleate, sorbitan palmitate, sorbitan sesquiisostearate, sorbitan stearate, etc.

[0081] Wetting agent

[0082] It is also desirable to include one or more wetting agents in the composition. If present, such wetting agents may range from about 0.001-25%, preferably about 0.005-20%, and more preferably about 0.1-15% by weight of the total composition. Examples of suitable wetting agents include glycols, sugars, and the like. Suitable glycols are in monomeric or polymeric form and include polyethylene glycol and polypropylene glycol, such as PEG 4-200, which is a polyethylene glycol having 4-200 ethylene oxide repeating units; and C 1-6 Alkylene glycols, such as propylene glycol, butylene glycol, pentylene glycol, etc. Suitable sugars (some of which are also polyols) are also suitable humectants. Examples of such sugars include glucose, fructose, honey, hydrogenated honey, inositol, maltose, mannitol, maltitol, sorbitol, sucrose, xylitol, xylose, etc. Urea is also suitable. Preferably, the humectant used in the composition of the present disclosure is C 1-6 (Preferred C 2-4 ) alkylene glycols, most particularly butanediol.

[0083] Plant Extracts

[0084] It may be desirable to include one or more plant extracts in the composition. If so, a suggested range is about 0.0001-10%, preferably about 0.0005-8%, more preferably about 0.001-5% by weight of the total composition. Suitable plant extracts include extracts from plants (herbs, roots, flowers, fruits, seeds) (e.g., flowers, fruits, vegetables, etc.), including yeast fermentation extract, Padina Pavonica extract, Thermus thermophilus fermentation extract, Camelina seed oil, Boswellia serrata extract, Olive extract, Aribodopsis Thaliana extract, Acacia Dealbata extract, Acer Saccharinum (Sugar Maple), Acidophilus, Calamus, Horse Chestnut, Agaricus, Agave, Agrimonia, Algae, Aloe, Citrus, Brassica, Cinnamon, Orange, Apple, Blueberry, Cranberry, Peach, Pear, Lemon, Lime, Pea, Seaweed, Caffeine, Green Tea, Chamomile, Willow Bark, Mulberry, Poppy, and those described in CTFA Cosmetic Ingredient Handbook, Eighth Edition, Vol. 2, pages 1646-1660.Further specific examples include, but are not limited to, Glycyrrhiza Glabra, Salix Nigra, Macrocycstis Pyrifera, Pyrus Malus, Saxifraga Sarmentosa, Vitis Vinifera, Morus Nigra, Scutellaria Baicalensis, Anthemis Nobilis, Salvia Sclarea, Rosmarinus Officianalis, Citrus Medica Limonum, Panax Ginseng, Siegesbeckia Orientalis, Fructus Mume, Ascophyllum Nodosum, Fermented Lysate, Glycine Soja Extract, Beta Vulgaris, Haberlea Rhodopensis, Polygonum Cuspidatum, Citrus AurantiumDulcis), grapes (Vitis Vinifera), Selaginella (Selaginella Tamariscina), hops (Humulus Lupulus), citrus (Citrus Reticulata) peel, pomegranate (Punica Granatum), seamendong (Asparagopsis), turmeric (Curcuma Longa), menyanthes (Menyanthes Trifoliata), sunflower (Helianthus Annuus), barley (Hordeum Vulgare), cucumber (Cucumis Sativus), flat moss (Evernia Prunastri), oak moss (Evernia Furfuracea) and mixtures thereof.

[0085] Granular materials

[0086] The compositions of the present disclosure may contain particulate material in the form of pigments, inert particles, or mixtures thereof. If present, the suggested range is about 0.01-75%, preferably about 0.5-70%, and more preferably about 0.1-65% by weight of the total composition. In the case where the composition may contain a mixture of pigments and powders, suitable ranges include about 0.01-75% pigment and 0.1-75% powder, such weights being based on the weight of the total composition.

[0087] The particulate material may be a colored or colorless powder. Suitable non-coloring powders include bismuth oxychloride, titanated mica, fumed silica, spherical silica, polymethyl methacrylate, micronized Teflon, boron nitride, acrylate copolymers, aluminum silicate, aluminum starch octenyl succinate, bentonite, calcium silicate, cellulose, chalk, corn starch, diatomaceous earth, fuller's earth, glyceryl starch, hectorite, hydrated silica, kaolin, magnesium aluminum silicate, magnesium trisilicate, maltodextrin, montmorillonite, microcrystalline cellulose, rice starch, silicon dioxide, talc, mica, titanium dioxide, zinc laurate, zinc myristate, zinc rosinate, alumina, attapulgite, calcium carbonate, calcium silicate, dextran, kaolin, nylon, silica silylate, silk powder, sericite, soy flour, tin oxide, titanium hydroxide, trimagnesium phosphate, walnut shell powder, or mixtures thereof. The above powders may be surface treated with lecithin, amino acids, mineral oils, silicones or various other agents, alone or in combination, which coat the powder surface and render the particles more lipophilic in nature.

[0088] Suitable pigments are organic or inorganic. Organic pigments are generally various aromatic types, including azo, indigo, triphenylmethane, anthraquinone and xanthine dyes, which are named D&C and FD&C blue, brown, green, orange, red, yellow, etc. Organic pigments are generally composed of insoluble metal salts (called lakes) of certified color additives. Inorganic pigments include iron oxide, ultramarine, chromium, chromium hydroxide colorants and mixtures thereof. Red, blue, yellow, brown, black iron oxides and mixtures thereof are suitable.

[0089] Vitamins

[0090] The composition of the present disclosure may contain vitamins and / or coenzymes. If so, it is recommended to be 0.001-10%, preferably 0.01-8%, and more preferably 0.05-5% by weight of the total composition. Suitable vitamins include ascorbic acid and its derivatives, such as ascorbyl palmitate, tetrahexyldecyl ascorbate, etc.; B vitamins, such as thiamine, riboflavin, pyridoxine, etc., and coenzymes, such as thiamine pyrophosphate, flavin adenine dinucleotide, folic acid, pyridoxal phosphate, tetrahydrofolate, etc. Vitamin E and its derivatives are also suitable, such as vitamin E acetate, nicotinic acid ester or other esters thereof. In addition, vitamin D and K are suitable.

[0091] Antioxidants and free radical scavengers

[0092] Antioxidants and free radical scavengers are particularly useful for providing protection against UV radiation that may cause increased scaling or textural changes in the stratum corneum and against other environmental agents that may cause skin damage. Such antioxidants / free radical scavengers include, for example, tocopheryl sorbate and other esters of tocopherol and tocopheryl sorbate.

[0093] Anti-inflammatory agents

[0094] Anti-inflammatory agents enhance skin appearance benefits by, for example, contributing to uniformity and acceptable skin tone and / or color. Optionally, anti-inflammatory agents include steroidal anti-inflammatory agents and non-steroidal anti-inflammatory agents. The steroidal anti-inflammatory agent may be hydrocortisone. So-called "natural" anti-inflammatory agents are also useful. For example, alpha bisabolol, aloe vera, Manjistha (extracted from plants of the genus Rubia (particularly Rubia)) and Guggal (extracted from plants of the genus Commiphora (particularly Indian Commiphora)), cola extract, chamomile and gorgonian extracts may also be used.

[0095] Antimicrobial Agents

[0096] As used herein, "antimicrobial agent" refers to a compound that is capable of destroying microorganisms, preventing the development of microorganisms, or preventing the pathogenic effects of microorganisms. Antimicrobial agents are useful, for example, in the control of acne. Preferred antimicrobial agents are benzoyl peroxide, erythromycin, tetracycline, clindamycin, azelaic acid, sulfur resorcinol, phenoxyethanol, and Irgasan. TM DP 300 (CibaGeigy Corp., USA). A safe and effective amount of antimicrobial agent may be added to the emulsion herein, preferably 0.001% to 10%, more preferably 0.01% to 5%, still more preferably 0.05% to 2%.

[0097] Chelating agents

[0098] As used herein, "chelating agent" refers to a compound that reacts to remove metal ions from a system by forming a complex so that the metal ions cannot readily participate in or catalyze chemical reactions. The inclusion of chelating agents is particularly useful for providing protection from UV radiation that may cause excessive scaling or changes in skin texture and from other environmental agents that may cause skin damage. Exemplary chelating agents useful herein are disclosed in U.S. Pat. No. 5,487,884, issued Jan. 30, 1996 to Bissett et al.; PCT Applications 91 / 16035 and 91 / 16034, issued Oct. 31, 1995 to Bush et al. Preferred chelating agents are furildioxime and its derivatives.

[0099] Silicone elastomer

[0100] The composition may include a non-emulsifying cross-linked organopolysiloxane elastomer. As used herein, the term "non-emulsifying" defines a cross-linked organopolysiloxane elastomer in which polyoxyalkylene units are absent. Such elastomers are used to reduce the tackiness / stickiness associated with skin conditioning agents.

[0101] The elastomer may be dimethicone / vinyl dimethicone crosspolymer, vinyl dimethicone / lauryl dimethicone crosspolymer, C30-C45 alkyl cetyl stearyl dimethicone / polycyclohexane oxide crosspolymer, and mixtures thereof.

[0102] Dimethicone / vinyl dimethicone crosspolymer is available from several suppliers, including Dow Corning (DC 9040 and DC 9041), General Electric (SFE 839), Shin Etsu (KSG-15, 16, 18 [dimethicone / phenyl vinyl dimethicone crosspolymer]), and Grant Industries (GRANSIL TM Crosslinked organopolysiloxane elastomers and methods for their preparation are further described in U.S. Patents 4,970,252, issued November 13, 1990 to Sakuta et al.; 5,760,116, issued June 2, 1998 to Kilgour et al.; and 5,654,362, issued August 5, 1997 to Schulz, Jr. et al.

[0103] Vinyl dimethicone / lauryl dimethicone crosspolymers include vinyl dimethicone / lauryl dimethicone crosspolymer and mineral oil (trade name KSG-41); vinyl dimethicone / lauryl dimethicone crosspolymer and isododecane (trade name KSG-42); vinyl dimethicone / lauryl dimethicone crosspolymer and triethylhexanoin (trade name KSG-43); vinyl dimethicone / lauryl dimethicone crosspolymer and squalane (trade name KSG-44). Each of these silicone elastomers named "KSG" can be obtained from Shinestu Chemical.

[0104] Commercially available cyclomethicone and C30-C45 alkyl cetyl stearyl dimethicone / polycyclohexane oxide crosspolymer are available under the trade name Velvasil 125 from GE Silicone.

[0105] Brightener

[0106] The compositions herein may further comprise from 0.001% to 10%, or from 0.1% to 5%, of a whitening agent. Non-limiting examples of suitable whitening agents are those that are compatible with aqueous compositions. Whitening agents may include active ingredients that not only change the appearance of the skin compared to pre-treatment, but also improve hyperpigmentation.

[0107] Useful whitening agents may include ascorbic acid compounds, azelaic acid, butylated hydroxyanisole, gallic acid and its derivatives, glycyrrhizic acid, hydroquinone, kojic acid, arbutin, mulberry extract, and mixtures thereof. It is believed that using a combination of whitening agents is advantageous because they may provide whitening benefits through different mechanisms.

[0108] The ascorbic acid compound can be an ascorbate or a derivative thereof. Exemplary water-soluble salt derivatives include, but are not limited to, L-ascorbic acid 2-glucoside, L-ascorbic acid phosphate ester salts, such as sodium L-ascorbic acid phosphate, potassium L-ascorbic acid phosphate, magnesium L-ascorbic acid phosphate, calcium L-ascorbic acid phosphate, aluminum L-ascorbic acid phosphate. L-ascorbic acid sulfate ester salts can also be used. Examples are sodium L-ascorbic acid sulfate, potassium L-ascorbic acid sulfate, magnesium L-ascorbic acid sulfate, calcium L-ascorbic acid sulfate, and aluminum L-ascorbic acid sulfate.

[0109] pH Adjuster

[0110] The composition may further comprise a pH adjusting agent to control the pH of the composition. In particular, the pH of the composition of the present disclosure is in the range of about 5 to about 8, or about 5.2 to about 7.8, or about 5.4 to about 7.6, such as about 5.4, about 5.6, about 5.8, about 6.0, about 6.2, about 6.4, about 6.6, about 6.8, about 7.0, about 7.2, about 7.4, about 7.6, and any range therebetween.

[0111] The composition may further comprise from about 0.01% to about 5%, or from about 0.1% to about 3%, or from about 0.3% to about 2%, or from about 0.4% to about 1.8%, and or from about 0.5% to about 1.6%, for example, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.1%, about 1.2%, about 1.3%, about 1.4%, about 1.5%, about 1.6%, and any range therebetween, of a pH adjuster, wherein the pH adjuster is selected from potassium hydroxide, sodium hydroxide, ammonium hydroxide, aminomethyl propanol, triethanolamine, tetrahydroxypropyl ethylenediamine, and any combination thereof, by weight of the composition.

[0112] When using a polymeric emulsifier and a pH adjuster, it may be desirable to optimize the ratio of the polymeric emulsifier to the pH adjuster. For example, the weight ratio of the polymeric emulsifier to the pH adjuster may be between about 1:5 and about 1:0.5, or between about 1:3 and about 1:1, such as about 1:3, about 1:2.5, about 1:2, about 1:1.5, about 1:1.1, and any range therebetween.

[0113] Thickener

[0114] If the emulsifier in the composition also acts as a thickener, the composition may further comprise a thickener (also referred to as a thickening agent) or an additional thickener. The composition may comprise from about 0.1% to about 5% or from about 0.2% to about 2% of a thickener or an additional thickener (when present). Suitable types of thickeners include, but are not limited to, carboxylic acid polymers, polyacrylamide polymers, sulfonated polymers, copolymers thereof, hydrophobically modified derivatives thereof, and mixtures thereof.

[0115] The thickener may be an acrylate cross-linked silicone copolymer network (sometimes also referred to as a "polyacrylate siloxane copolymer network"). Suitable thickeners may also generally include carboxylic acid polymers, polyacrylamide polymers or copolymers, sulfonated polymers, gums, clays, cellulose or modified cellulose compositions, and the like.

[0116] The composition of the present invention may also be "essentially composed of" an extract from Hibiscus, an extract from damaged plant parts of Moringa, oligopeptides, an extract from Laminaria and whey protein, which refers to a composition that contains the five ingredients mentioned and only contains additional ingredients that do not affect the basic and novel characteristics of the composition, such as any ingredients mentioned in the other ingredients section, and inert ingredients that do not affect the collagen stimulating activity of the composition.

[0117] B. method

[0118] In some embodiments, the present disclosure relates to methods of stimulating collagen synthesis in skin cells by administering a composition comprising an extract from Hibiscus and an extract from damaged plant parts of Moringa.

[0119] In some aspects, the present disclosure relates to methods of stimulating collagen synthesis in skin cells by applying a composition comprising an extract from Hibiscus, an extract from damaged plant parts of Moringa, and a complex of actives comprising oligopeptides, an extract from Laminaria, and whey protein.

[0120] The present disclosure will be further described in conjunction with the following examples, which are set forth for purposes of illustration only.

[0121] experiment

[0122] Example 1

[0123] The ability of the comparative samples to stimulate collagen production in normal human dermal fibroblasts ("NHDF") was tested. NHDF from a 62-year-old donor were plated at a density of 50,000 cells / well in 6-well plates and incubated overnight under standard conditions (37°C / 5% CO□). Fibroblasts were grown in DMEM medium supplemented with 10% bovine calf serum and 1% penicillin-streptomycin.

[0124] Comparable extracts were prepared from flowers harvested at different times and extracted using the same method. ) Comparable Extract A was obtained from dark pink Hibiscus mutabilis flowers. Comparable Extract B was prepared from dark pink Hibiscus mutabilis flowers. And Comparable Extract C was obtained from red Hibiscus mutabilis flowers.

[0125] Samples were treated with comparable extracts A, B or C in fully supplemented medium at 0.0021%, 0.0105% or 0.021% active doses. Plates were incubated for 96 hours under standard conditions (37°C / 5% CO□). After incubation, supernatants were harvested and stored at -80°C.

[0126] After 96 hours of incubation, the supernatant was collected and cell counts were performed to determine cell viability. Each sample was washed once with PBS and then given 0.25% trypsin. After the cells were detached from the 6-well plate, medium was given to each sample to neutralize the trypsin. Cell counts were performed using Vicell.

[0127] Collagen I production was assessed using Pro Collagen Type I ELISA (Takara MK101) according to the supplier's protocol (Takara Bio, MK101). Optical density (OD) was measured at 450 nm.

[0128] All samples were statistically analyzed using one-way ANOVA with Dunnett's post-test using Graphpad InStat. The results are described below:

[0129]

[0130]

[0131] This experiment shows the critical importance of harvest time when obtaining plant extracts and that precision is necessary to deliver optimal performance to the skin. Extracts from young Hibiscus mutabilis flowers (white flowers and / or light pink flowers) showed significantly better efficacy in stimulating collagen production at all active concentrations.

[0132] Example 2

[0133] The composition samples were tested for their ability to stimulate collagen production in normal human dermal fibroblasts ("NHDF"). NHDF from elderly donors (61 and 62 years old) were plated at a density of 50,000 cells / well in 6-well plates and incubated overnight under standard conditions (37°C / 5% CO□). Fibroblasts were grown in DMEM medium supplemented with 10% bovine calf serum and 1% penicillin-streptomycin.

[0134] The composition samples were prepared with the ingredients and concentrations listed in the table below and were numbered as follows: (1) an extract of Hibiscus mutabilis; (2) an extract of Hibiscus mutabilis and an extract of Moringa oleifera; (3) a complex of an extract of Moringa oleifera, active ingredients (acetyl hexapeptide-8, an extract of Laminaria digitata, and whey protein); (4) a complex of an extract of Hibiscus mutabilis, an extract of Moringa oleifera, active ingredients (acetyl hexapeptide-8, an extract of Laminaria digitata, and whey protein).

[0135]

[0136] Controls and samples were tested in fully supplemented medium. Plates were incubated under standard conditions (37°C / 5% CO□) for 96 hours. After incubation, supernatants were harvested and stored at -80°C.

[0137] After 96 hours of incubation and collection of supernatant, cell counts were performed to determine cell viability. Each sample was washed once with PBS and then given 0.25% trypsin. After cells were detached from the 6-well plate, culture medium was given to each sample to neutralize trypsin. Cell counts were performed with Vicell.

[0138] Collagen I production was assessed using Pro Collagen Type I ELISA (Takara MK101) according to the supplier's protocol (Takara Bio, MK101). Optical density (OD) was measured at 450 nm.

[0139] All samples were statistically analyzed using one-way ANOVA with Dunnett's post-test using Graphpad InStat. The results are described below:

[0140]

[0141] Although the extract of Moringa oleifera itself has no activity in stimulating collagen production, the extract of Hibiscus mume induced a 59% increase in collagen production. Furthermore, the combination of the extract of Hibiscus mume and the extract of Moringa oleifera synergistically induced a 483% increase in collagen production. In addition, the combination of the extract of Hibiscus mume, the extract of Moringa oleifera and the complex of actives induced an 888% increase, while the combination of the extract of Moringa oleifera and the complex of actives only induced a 510% increase.

[0142] Other Implementations

[0143] Although the present invention has been described in conjunction with preferred embodiments, it is not intended that the scope of the present invention be limited to the specific forms set forth, but rather, it is intended to cover such substitutions, modifications and equivalents that may be included in the spirit and scope of the present invention as defined by the appended claims. Therefore, other embodiments (including those embodiments that can be easily modified by those skilled in the art in light of this disclosure) are also within the scope of the claims.

Claims

1. A cosmetic composition for skin care, comprising an extract from Hibiscus mutabilis and an extract from damaged plant parts of Moringa oleifera, wherein the extract from Hibiscus mutabilis is present in an amount ranging from 0.001-5% by weight of the total composition, the extract from damaged plant parts of Moringa oleifera is present in an amount ranging from 0.001-5% by weight of the total composition, and the extract from Hibiscus mutabilis is obtained from Hibiscus mutabilis white flowers and / or light pink flowers.

2. The composition of claim 1, wherein the extract from Hibiscus mutabilis comprises at least 2 ppm of N-(1-deoxy-1-fructosyl) and / or at least 145 ppm of cyperoside epoxide.

3. The composition of claim 1, wherein the extract from Hibiscus mume is a hydroglycolic extract.

4. The composition of claim 1, wherein the extract from Hibiscus mutabilis contains less than 60 ppm of an isomer of citric acid having a molar mass of 192 Da.

5. The composition of claim 1, wherein the extract from Hibiscus mutabilis comprises at least 50% carbohydrates by dry weight of the extract.

6. The composition of claim 5, wherein at least 30% by dry weight of the carbohydrates present in the extract from Hibiscus mutabilis are oligosaccharides and polysaccharides having a molar mass between 360-1,620 Da.

7. The composition of claim 1, wherein the extract from Hibiscus mutabilis comprises minerals and / or proteins.

8. The composition according to claim 7, wherein the protein is a peptide with a molar mass below 2,000 Da.

9. The composition of claim 7, wherein the extract from Hibiscus mutabilis comprises less than 20% minerals.

10. The composition of claim 7, wherein the extract from Hibiscus mutabilis comprises less than 20% protein.

11. The composition of claim 1, wherein the concentration of moringa isothiocyanates of the extract from damaged plant parts of Moringa oleifera ranges from 0.5% to 3.0% by weight of the total extract.

12. The composition of claim 1, further comprising a complex of actives comprising at least one oligopeptide, at least one extract from Laminaria and whey protein.

13. The composition of claim 12, wherein the oligopeptide is present in an amount ranging from 0.000001-5% by weight of the total composition.

14. The composition of claim 12, wherein the oligopeptide is acetyl hexapeptide-8.

15. The composition of claim 12, wherein the at least one extract from Laminaria is present in an amount ranging from 0.0001-5% by weight of the total composition.

16. The composition according to claim 15, wherein the extract from the genus Laminaria is an extract from Laminaria digitata, and its laminarin content and / or mannitol content is in the range of 0.5-3% by weight of the total extract.

17. The composition of claim 12, wherein the whey protein is present in an amount ranging from 0.01-5% by weight of the total composition.

18. A non-therapeutic method for stimulating collagen synthesis in skin cells by administering a composition according to any one of claims 1 to 17.

19. The method of claim 18, wherein the composition further comprises a complex of active ingredients comprising oligopeptides, an extract from Laminaria and whey protein.

20. The method of claim 18, wherein the composition is administered once or twice daily.

21. The method of claim 19, wherein the oligopeptide is acetyl hexapeptide-8 and the Laminaria is Laminaria digitata.

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