A method for preparing Chinese herbal medicine extract based on low-temperature wall-breaking technology and its application in preparing cosmetics

Abstract

The present invention specifically discloses a method for preparing Chinese herbal medicine extracts based on low-temperature wall-breaking technology and its application in preparing cosmetics. The method comprises the following steps: (1) taking Chinese herbal medicine and crushing it to obtain Chinese herbal medicine powder, and soaking the Chinese herbal medicine powder in water to obtain water-containing herbal medicine powder; (2) freezing the water-containing herbal medicine powder at -20~-40°C for 30~60min to obtain frozen Chinese herbal medicine powder; (3) treating the frozen Chinese herbal medicine powder at -160~-200°C for 5~10min to obtain ultra-low temperature wall-breaking Chinese herbal medicine powder; (4) thawing the ultra-low temperature wall-breaking Chinese herbal medicine powder, and extracting it with a solvent after thawing; after the extraction is completed, filtering and separating the extract, and concentrating and drying the extract to obtain the Chinese herbal medicine extract. Studies have shown that the Chinese herbal medicine extract prepared by the method of the present invention has a tyrosinase inhibitory effect; therefore, using it as an effective ingredient to prepare cosmetics with whitening effect has important application value.

Description

Technical Field

[0001] The present invention relates to the technical fields of biomedicine and daily chemical products, and particularly relates to a method for preparing Chinese herbal medicine extracts based on cryogenic cell wall breaking technology and its application in the preparation of cosmetics. Background Art

[0002] Chinese herbal medicine extracts are a collection of active ingredients obtained by extracting and separating Chinese herbal medicines or Chinese herbal medicine compounds using extraction and separation processes; they have broad medical activities and are therefore widely used in the natural medicine and health product market. Since Chinese herbal medicine extracts from natural sources also have activities such as whitening, antioxidant, anti-aging, and wrinkle removal; they have also been widely used in cosmetics.

[0003] However, the extracts currently used in cosmetics are usually obtained by conventional methods such as heating under reflux and ultrasonic extraction; they cannot fully extract the active ingredients in Chinese herbal medicines. Cryogenic cell wall breaking technology can enable as much as possible of the active ingredients in Chinese herbal medicines to dissolve out, thereby improving the corresponding efficacy of Chinese herbal medicine extracts. However, cryogenic cell wall breaking technology has not yet been widely applied to the development of cosmetic raw materials; therefore, using cryogenic cell wall breaking technology to prepare cosmetic raw materials has important application value. Summary of the Invention

[0004] In order to overcome at least one technical problem existing in the prior art, the present invention provides a method for preparing Chinese herbal medicine extracts based on cryogenic cell wall breaking technology and its application in the preparation of cosmetics.

[0005] The above technical problems to be solved by the present invention are realized by adopting the following technical solutions:

[0006] The present invention first provides a method for preparing Chinese herbal medicine extracts based on cryogenic cell wall breaking technology, which is characterized by comprising the following steps:

[0007] (1) Take Chinese herbal medicines, crush them to obtain Chinese herbal medicine powder, and obtain water-containing Chinese herbal medicine powder after wetting the Chinese herbal medicine powder with water;

[0008] Chinese herbal medicine powder;

[0009] (2) Freeze the water-containing Chinese herbal medicine powder at -20 to -40 °C for 30 to 60 minutes to obtain frozen Chinese herbal medicine powder;

[0010] (3) Process the frozen Chinese herbal medicine powder at -160 to -200 °C for 5 to 10 minutes to obtain ultra-low temperature cell wall broken Chinese herbal medicine powder;

[0011] (4)Thaw the ultralow temperature broken Chinese herbal medicine powder, and after thawing, extract it with a solvent; after the extraction is completed, filter and separate the extract, and concentrate and dry the extract to obtain the Chinese herbal medicine solvent extract; take the Chinese herbal medicine solvent extract to obtain the described Chinese herbal medicine extract.

[0012] The present invention provides a brand-new preparation method of Chinese herbal medicine extract; research shows that after the Chinese herbal medicine raw material is first subjected to the low-temperature wall-breaking treatment described in the present invention and then prepared by solvent extraction, the obtained Chinese herbal medicine extract has a significantly higher tyrosinase inhibitory effect than the Chinese herbal medicine extract directly obtained by solvent extraction without low-temperature wall-breaking treatment.

[0013] Preferably, in step (1), the weight ratio of the Chinese herbal medicine powder to water is 1:1 to 3.

[0014] Most preferably, in step (1), the weight ratio of the Chinese herbal medicine powder to water is 1:2.

[0015] Preferably, the Chinese herbal medicine described in step (1) is selected from one or more mixtures of Salvia miltiorrhiza, Astragalus membranaceus, Rhodiola rosea, and Houttuynia cordata.

[0016] Most preferably, the Chinese herbal medicine described in step (1) is selected from Houttuynia cordata.

[0017] Most preferably, in step (2), the water-containing Chinese herbal medicine powder is frozen at -30°C for 40 minutes to obtain the frozen Chinese herbal medicine powder.

[0018] Most preferably, in step (3), the frozen Chinese herbal medicine powder is treated at -196°C for 8 minutes to obtain the ultralow temperature broken Chinese herbal medicine powder.

[0019] Preferably, the solvent described in step (4) is selected from one or more mixtures of water, alcohol, ethyl acetate, and n-hexane.

[0020] Preferably, the solvent described in step (4) is composed of ethyl acetate and n-hexane.

[0021] Further preferably, the volume ratio of ethyl acetate to n-hexane is 2 to 4:1.

[0022] Most preferably, the volume ratio of ethyl acetate to n-hexane is 3:1.

[0023] Research shows that taking Houttuynia cordata as the raw material, after first undergoing low-temperature wall-breaking treatment and then extracting with a solvent composed of ethyl acetate and n-hexane, the obtained Chinese herbal medicine solvent extract has a much higher tyrosinase inhibitory effect than the Chinese herbal medicine solvent extract obtained by extracting with other solvents or combinations of other solvents.

[0024] Preferably, the dosage ratio of the ultralow temperature broken Chinese herbal medicine powder to the solvent in step (4) is 1 kg:5 to 10 L.

[0025] Most preferably, in step (4), the dosage ratio of the ultra-low temperature broken Chinese herbal medicine powder to the solvent is 1 kg: 8 L.

[0026] Preferably, the method for preparing the Chinese herbal medicine extract based on the low-temperature breaking technology further comprises the following steps: subjecting the Chinese herbal medicine solvent extract to a silica gel column, first eluting and removing impurities with a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:4-7; then eluting with a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:17-19, collecting the eluate eluted by the mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:17-19, and concentrating and drying to obtain the Chinese herbal medicine extract.

[0027] Most preferably, the method for preparing the Chinese herbal medicine extract based on the low-temperature breaking technology further comprises the following steps: subjecting the Chinese herbal medicine solvent extract to a silica gel column, first eluting and removing impurities with a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:6; then eluting with a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:18, collecting the eluate eluted by the mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:18, and concentrating and drying to obtain the Chinese herbal medicine extract.

[0028] The inventor further found in the research that the Chinese herbal medicine extract prepared by further eluting the Chinese herbal medicine solvent extract under the silica gel column elution conditions of the present invention has a further significant improvement in its tyrosinase inhibitory effect compared with the Chinese herbal medicine solvent extract.

[0029] In addition, the inventor needs to explain that the silica gel column elution conditions of the present invention are very crucial; only the Chinese herbal medicine extract prepared by further eluting the Chinese herbal medicine solvent extract under the silica gel column elution conditions of the present invention can have a further significant improvement in its tyrosinase inhibitory effect compared with the Chinese herbal medicine solvent extract; however, the Chinese herbal medicine extract prepared by eluting the Chinese herbal medicine solvent extract under other silica gel column elution conditions cannot have a further significant improvement in its tyrosinase inhibitory effect compared with the Chinese herbal medicine solvent extract.

[0030] The present invention also provides a Chinese herbal medicine extract prepared by the above method.

[0031] The present invention also provides an application of the above Chinese herbal medicine extract in preparing a cosmetic having an inhibitor against tyrosinase.

[0032] The present invention also provides an application of the above Chinese herbal medicine extract in preparing a cosmetic having a whitening effect.

[0033] Beneficial effects: The present invention provides a brand-new method for preparing a Chinese herbal medicine extract; research shows that after the Chinese herbal medicine raw materials are first subjected to the low-temperature cell wall breaking treatment described in the present invention and then prepared into a Chinese herbal medicine extract by solvent extraction; its tyrosinase inhibitory effect is significantly higher than that of the Chinese herbal medicine extract directly obtained by solvent extraction without low-temperature cell wall breaking treatment. Since the Chinese herbal medicine extract prepared by the method described in the present invention has a tyrosinase inhibitory effect; therefore, using it as an active ingredient for preparing cosmetics with whitening effect has important application value. Detailed implementation manners

[0034] The following combines specific embodiments to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0035] Example 1 Method for preparing Chinese herbal medicine extract based on low-temperature cell wall breaking technology

[0036] (1) Take the Chinese herbal medicine, crush it and pass it through a 100-mesh sieve to obtain Chinese herbal medicine powder. After wetting the Chinese herbal medicine powder with water, obtain water-containing Chinese herbal medicine powder; wherein, the weight ratio of the Chinese herbal medicine powder to water is 1:2; the Chinese herbal medicine is dried Houttuynia cordata.

[0037] (2) Freeze the water-containing Chinese herbal medicine powder at -30°C for 40 min to obtain frozen Chinese herbal medicine powder.

[0038] (3) Quickly (within 30 seconds) put the frozen Chinese herbal medicine powder into liquid nitrogen for rapid cooling, and treat it at -196°C for 8 min to obtain ultra-low temperature cell wall broken Chinese herbal medicine powder.

[0039] (4) Thaw the ultra-low temperature cell wall broken Chinese herbal medicine powder with microwave. After thawing, extract it with a solvent at 60°C by ultrasonic wave (ultrasonic frequency is 40 kHz, ultrasonic power is 1 kW) for 1 h; after the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a Chinese herbal medicine solvent extract; take the Chinese herbal medicine solvent extract to obtain the Chinese herbal medicine extract.

[0040] In step (4), the dosage ratio of the ultra-low temperature cell wall broken Chinese herbal medicine powder to the solvent is 1 kg:8 L; the solvent is water.

[0041] Example 2 Method for preparing Chinese herbal medicine extract based on low-temperature cell wall breaking technology

[0042] The difference between Example 2 and Example 1 is that the solvent used in step (4) is methanol with a volume fraction of; the rest are the same as in Example 1.

[0043] Example 3 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0044] The difference between Example 3 and Example 1 is that the solvent used in step (4) is ethyl acetate; the rest are the same as in Example 1.

[0045] Example 4 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0046] The difference between Example 4 and Example 1 is that the solvent used in step (4) is n-hexane; the rest are the same as in Example 1.

[0047] Example 5 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0048] The difference between Example 5 and Example 1 is that the solvent used in step (4) is composed of ethyl acetate and n-hexane with a volume ratio of 3:1; the rest are the same as in Example 1.

[0049] Example 6 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0050] The difference between Example 6 and Example 1 is that the solvent used in step (4) is composed of water and methanol with a volume ratio of 3:1; the rest are the same as in Example 1.

[0051] Example 7 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0052] The difference between Example 7 and Example 1 is that the solvent used in step (4) is composed of ethyl acetate and methanol with a volume ratio of 3:1; the rest are the same as in Example 1.

[0053] Example 8 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0054] The difference between Example 8 and Example 1 is that the solvent used in step (4) is composed of methanol and n-hexane with a volume ratio of 3:1; the rest are the same as in Example 1.

[0055] Example 9 Method for Preparing Chinese Herbal Medicine Extract Based on Low-Temperature Wall-Breaking Technology

[0056] (1) Take the Chinese herbal medicine, crush it and sieve it through a 100-mesh sieve to obtain Chinese herbal medicine powder. After wetting the Chinese herbal medicine powder with water, obtain water-containing Chinese herbal medicine powder; among them, the weight ratio of the Chinese herbal medicine powder to water is 1:2; the Chinese herbal medicine is dried Houttuynia cordata.

[0057] (2) Freeze the water-containing Chinese herbal medicine powder at -30°C for 40 min to obtain frozen Chinese herbal medicine powder.

[0058] (3) Rapidly (within 30 seconds) place the frozen Chinese herbal medicine powder into liquid nitrogen for rapid cooling, and treat it at -196°C for 8 minutes to obtain ultra-low temperature broken-wall Chinese herbal medicine powder;

[0059] (4) Thaw the ultra-low temperature broken-wall Chinese herbal medicine powder with microwave. After thawing, extract it with a solvent at 60°C by ultrasonic wave (ultrasonic frequency is 40 kHz, ultrasonic power is 1 kW) for 1 hour; After the extraction is completed, filter and separate the extract, and concentrate and dry the extract to obtain the Chinese herbal medicine solvent extract; Among them, the dosage ratio of the ultra-low temperature broken-wall Chinese herbal medicine powder to the solvent in step (4) is 1 kg:8 L; The solvent is composed of ethyl acetate and n-hexane with a volume ratio of 3:1;

[0060] (5) Take the Chinese herbal medicine solvent extract and load it onto a silica gel column (the silica gel column is filled with 200-300 mesh silica gel with a weight 40 times that of the Chinese herbal medicine solvent extract), first elute and remove impurities with 3 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:6; Then elute with 5 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:18, collect the eluate eluted by the mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:18, and concentrate and dry it to obtain the Chinese herbal medicine extract.

[0061] Comparative Example 1 Method for preparing Chinese herbal medicine extract based on low-temperature broken-wall technology

[0062] (1) Take the Chinese herbal medicine, crush it and pass it through a 100-mesh sieve to obtain Chinese herbal medicine powder; The Chinese herbal medicine is dried Houttuynia cordata;

[0063] (2) Extract the Chinese herbal medicine powder with a solvent at 60°C by ultrasonic wave (ultrasonic frequency is 40 kHz, ultrasonic power is 1 kW) for 1 hour; After the extraction is completed, filter and separate the extract, and concentrate and dry the extract to obtain the Chinese herbal medicine solvent extract; Take the Chinese herbal medicine solvent extract to obtain the Chinese herbal medicine extract; Among them, the dosage ratio of the Chinese herbal medicine powder to the solvent is 1 kg:8 L; The solvent is water.

[0064] Comparative Example 2 Method for preparing Chinese herbal medicine extract based on low-temperature broken-wall technology

[0065] (1) Take the Chinese herbal medicine, crush it and pass it through a 100-mesh sieve to obtain Chinese herbal medicine powder, and obtain water-containing Chinese herbal medicine powder after wetting the Chinese herbal medicine powder with water; Among them, the weight ratio of the Chinese herbal medicine powder to water is 1:2; The Chinese herbal medicine is dried Houttuynia cordata;

[0066] (2) Freeze the water-containing Chinese herbal medicine powder at -30°C for 40 minutes to obtain frozen Chinese herbal medicine powder;

[0067] (3) Rapidly (within 30 seconds) place the frozen Chinese herbal medicine powder into liquid nitrogen for rapid cooling, and treat it at -196°C for 8 minutes to obtain the ultra-low temperature broken-wall Chinese herbal medicine powder;

[0068] (4) Thaw the ultra-low temperature broken-wall Chinese herbal medicine powder with microwave, and after thawing, perform ultrasonic extraction with a solvent at 60°C for 1 hour; after the extraction is completed, filter and separate the extract, and concentrate and dry the extract to obtain the Chinese herbal medicine solvent extract; wherein, the dosage ratio of the ultra-low temperature broken-wall Chinese herbal medicine powder to the solvent is 1 kg:8 L; the solvent is composed of ethyl acetate and n-hexane with a volume ratio of 3:1;

[0069] (5) Take the Chinese herbal medicine solvent extract and load it onto a silica gel column (the silica gel column is filled with 200-300 mesh silica gel with a weight 40 times that of the Chinese herbal medicine solvent extract), first elute and remove impurities with 3 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:15; then elute with 5 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:25, collect the eluate eluted by the mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:25, and concentrate and dry it to obtain the Chinese herbal medicine extract.

[0070] The difference between Comparative Example 2 and Example 5 lies in the different elution conditions of the silica gel column.

[0071] Comparative Example 3 Method for preparing Chinese herbal medicine extract based on low-temperature broken-wall technology

[0072] (1) Take the Chinese herbal medicine, crush it and pass it through a 100-mesh sieve to obtain the Chinese herbal medicine powder, and soak the Chinese herbal medicine powder with water to obtain the water-containing Chinese herbal medicine powder; wherein, the weight ratio of the Chinese herbal medicine powder to water is 1:2; the Chinese herbal medicine is dried houttuynia cordata;

[0073] (2) Freeze the water-containing Chinese herbal medicine powder at -30°C for 40 minutes to obtain the frozen Chinese herbal medicine powder;

[0074] (3) Rapidly (within 30 seconds) place the frozen Chinese herbal medicine powder into liquid nitrogen for rapid cooling, and treat it at -196°C for 8 minutes to obtain the ultra-low temperature broken-wall Chinese herbal medicine powder;

[0075] (4) Thaw the ultra-low temperature broken-wall Chinese herbal medicine powder with microwave, and after thawing, perform ultrasonic extraction with a solvent at 60°C for 1 hour; after the extraction is completed, filter and separate the extract, and concentrate and dry the extract to obtain the Chinese herbal medicine solvent extract; wherein, the dosage ratio of the ultra-low temperature broken-wall Chinese herbal medicine powder to the solvent is 1 kg:8 L; the solvent is composed of ethyl acetate and n-hexane with a volume ratio of 3:1;

[0076] (5) Take the silica gel column with the solvent extract of Chinese herbal medicine (the silica gel column is filled with silica gel of 200-300 mesh, which is 40 times the weight of the solvent extract of Chinese herbal medicine). First, elute and remove impurities with 3 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:30. Then, elute with 5 column volumes of a mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:50. Collect the eluate eluted by the mixed organic solvent composed of chloroform and acetone with a volume ratio of 100:50. After concentration and drying, the said Chinese herbal medicine extract can be obtained.

[0077] The difference between Comparative Example 3 and Example 5 lies in the different elution conditions of the silica gel column.

[0078] Experimental Example: Tyrosinase Inhibition Activity Test

[0079] Add 50 μL of L-DOPA solution, the sample solution to be tested and / or 50 μL of tyrosinase solution into a 96-well plate; after mixing evenly, place it in a water bath at 30 °C for reaction for 25 min; then measure the absorbance A (490 nm). Each group of tests is repeated 3 times, and the absorbance of each group of tests takes the average value of 3 measurements; calculate the tyrosinase inhibition rate; the calculation formula of the tyrosinase inhibition rate is: tyrosinase inhibition rate = [1 - (A X1 - A X2 ) / (A Y1 - A Y2 )] x 100%; where A X1 is the absorbance measured when adding the tyrosinase solution and the sample solution to be tested; A X2 is the absorbance measured when not adding the tyrosinase solution (replaced with an equal amount of phosphate buffer solution) but adding the sample solution to be tested; A Y1 is the absorbance measured when adding the tyrosinase solution but not adding the sample solution to be tested (replaced with an equal amount of phosphate buffer solution); A Y2 is the absorbance measured when neither adding the tyrosinase solution nor adding the sample solution to be tested (replaced with an equal amount of phosphate buffer solution). The test results are shown in Table 1.

[0080] In the above experiment, the concentration of the L-DOPA solution is 1 g / L, which is prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8; the concentration of the sample solution to be tested is 1 g / L, which is prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8; the concentration of the tyrosinase solution is 100 U / mL, which is prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8.

[0081] The said samples to be tested are respectively the Chinese herbal medicine extracts prepared by the methods of Examples 1-9 and Comparative Examples 1-3.

[0082] Table 1. Tyrosinase Inhibition Activity Test Results

[0083]

[0084] As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the Chinese herbal medicine extract prepared by the method of Example 1 is significantly higher than that of the Chinese herbal medicine extract prepared by the method of Comparative Example 1. This shows that after the Chinese herbal medicine raw materials are first subjected to the low-temperature cell wall breaking treatment described in the present invention and then extracted with a solvent, the tyrosinase inhibition effect of the obtained Chinese herbal medicine extract is significantly higher than that of the Chinese herbal medicine extract directly obtained by solvent extraction without low-temperature cell wall breaking treatment.

[0085] As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the Chinese herbal medicine extract prepared by the method of Example 5 is much higher than that of the Chinese herbal medicine extracts prepared by the methods of Examples 1 to 4. This shows that: taking Houttuynia cordata as the raw material, after first undergoing low-temperature cell wall breaking treatment and then extracting with a solvent composed of ethyl acetate and n-hexane, the tyrosinase inhibition effect of the obtained Chinese herbal medicine solvent extract is much higher than that of the Chinese herbal medicine solvent extract obtained by extracting with a single water, alcohol, ethyl acetate or n-hexane; the solvent composed of ethyl acetate and n-hexane can synergistically improve the tyrosinase inhibition effect of the obtained Chinese herbal medicine solvent extract.

[0086] As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the Chinese herbal medicine extracts prepared by the methods of Examples 6 to 8 has not been significantly improved compared with that of the Chinese herbal medicine extracts prepared by the methods of Examples 1 to 4. This shows that: taking Houttuynia cordata as the raw material, after first undergoing low-temperature cell wall breaking treatment, only when the Chinese herbal medicine solvent extract is obtained by extracting with a solvent composed of ethyl acetate and n-hexane can its tyrosinase inhibition effect be much higher than that of the Chinese herbal medicine solvent extract obtained by extracting with a single water, alcohol, ethyl acetate or n-hexane; the solvent composed of ethyl acetate and n-hexane can synergistically improve the tyrosinase inhibition effect of the obtained Chinese herbal medicine solvent extract.

[0087] As can be seen from the experimental data in Table 1, the tyrosinase inhibition rate of the Chinese herbal medicine extract prepared by the method of Example 9 is much higher than that of the Chinese herbal medicine extract prepared by the method of Example 5. This shows that:

[0088] The Chinese herbal medicine extract prepared by further eluting the Chinese herbal medicine solvent extract under the silica gel column elution conditions described in the present invention has a further significantly improved tyrosinase inhibition effect compared with the Chinese herbal medicine solvent extract.

[0089] It can be seen from the experimental data in Table 1 that for the Chinese herbal medicine extract prepared by the method of Comparative Example 2, its tyrosinase inhibition rate has not been significantly improved compared with the Chinese herbal medicine extract prepared by the method of Example 5; for the Chinese herbal medicine extract prepared by the method of Comparative Example 3, its tyrosinase inhibition rate has not been improved but decreased compared with the Chinese herbal medicine extract prepared by the method of Example 5; this shows that: the silica gel column elution conditions described in the present invention are very crucial; only the Chinese herbal medicine extract prepared by further passing through the silica gel column elution conditions described in the present invention from the Chinese herbal medicine solvent extract can its tyrosinase inhibition effect be further significantly improved compared with the Chinese herbal medicine solvent extract; however, for the Chinese herbal medicine extract prepared by the Chinese herbal medicine solvent extract through other silica gel column elution conditions, its tyrosinase inhibition effect cannot be further significantly improved compared with the Chinese herbal medicine solvent extract.

[0090] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims in the present invention.

[0091] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for preparing Chinese herbal medicine extracts based on low-temperature wall-breaking technology, characterized in that: The following steps are included: (1) crushing the Chinese herbal medicine to obtain Chinese herbal medicine powder, and soaking the Chinese herbal medicine powder in water to obtain water-containing herbal medicine powder; (2) Freezing the herbal medicine powder in water at -20 to -40°C for 30 to 60 minutes to obtain frozen herbal medicine powder; (3) treating the frozen Chinese herbal medicine powder at -160~-200°C for 5~10 minutes to obtain ultra-low temperature wall-broken Chinese herbal medicine powder; (4) thawing the ultra-low temperature broken wall Chinese herbal medicine powder, and extracting it with a solvent after thawing; filtering and separating the extract after the extraction, and concentrating and drying the extract to obtain a Chinese herbal medicine solvent extract; taking the Chinese herbal medicine solvent extract to obtain the Chinese herbal medicine extract; The Chinese herbal medicine in step (1) is selected from Houttuynia cordata; In step (1), the weight ratio of Chinese herbal medicine powder to water is 1:1-3; The solvent in step (4) is composed of ethyl acetate and n-hexane; wherein the volume ratio of ethyl acetate to n-hexane is 2-4:1; The Chinese herbal medicine extract is a Chinese herbal medicine extract with tyrosinase inhibitor effect.

2. The method for preparing Chinese herbal medicine extracts based on low-temperature wall-breaking technology according to claim 1, characterized in that: In step (1), the weight ratio of Chinese herbal medicine powder to water is 1:

2.

3. The method for preparing Chinese herbal medicine extracts based on low-temperature wall-breaking technology according to claim 1, characterized in that: In step (2), the water-containing herbal medicine powder is frozen at -30°C for 40 minutes to obtain frozen herbal medicine powder.

4. The method for preparing Chinese herbal medicine extracts based on low-temperature wall-breaking technology according to claim 1, characterized in that: In step (3), the frozen Chinese herbal medicine powder is treated at -196°C for 8 minutes to obtain ultra-low temperature wall-broken Chinese herbal medicine powder.

5. The Chinese herbal medicine extract prepared by the method according to any one of claims 1 to 4.

6. Use of the Chinese herbal extract according to claim 5 in the preparation of cosmetics having tyrosinase inhibitor effect.

7. Use of the Chinese herbal medicine extract according to claim 5 in preparing cosmetics with whitening effect.

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