A traditional Chinese medicine composition and use thereof

Abstract

The scheme discloses a traditional Chinese medicine composition and application thereof in the cosmetic technical field, and the traditional Chinese medicine composition is composed of 5-35 parts of malus micromalus, 10-45 parts of pyracantha fortuneana, 5-45 parts of dendrobium nobile and 5-45 parts of ainsliaea fragrans, and can be applied to various skin care products such as face masks and face creams. The traditional Chinese medicine composition has good anti-inflammatory activity, can significantly improve the cell inflammatory factor level, and well solves the problems of poor anti-inflammatory effect and easy allergic phenomenon of common treatment acne skin care products.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and specifically relates to a traditional Chinese medicine composition and its uses. Background Technology

[0002] Acne is a chronic inflammatory skin disease affecting the hair follicles and sebaceous glands. In traditional Chinese medicine, it falls under the categories of "acne," "lung wind acne," "acne," and "facial pimples." Clinically, it is characterized by comedones, papules, pustules, nodules, and cysts, commonly occurring on the face, chest, and back, and is prone to recurrence. The prevalence of acne is increasing year by year. If treatment is not timely or the wrong medication is chosen, it may lead to pigmentation or scarring, affecting appearance and even causing psychological problems. The pathogenesis of acne is complex, involving four classic factors: genetics, androgen-induced excessive sebum secretion, proliferation of Propionibacterium acnes, keratinization of the pilosebaceous duct, and inflammation and immunity. Among these, Propionibacterium acnes, inflammatory response, and sebum production are the main links.

[0003] Currently, Western medicine treatments primarily rely on antibiotics and retinoids, which, while possessing antibacterial and anti-inflammatory effects, have adverse reactions and cannot reduce recurrence rates. In contrast, traditional Chinese medicine offers many natural and safe remedies with proven efficacy and fewer side effects, suitable for human skin. Therefore, exploring traditional Chinese medicine for acne treatment has significant economic and social value. Summary of the Invention

[0004] The present invention aims to provide a traditional Chinese medicine composition for treating acne that is effective, has few side effects, and is natural and safe, in order to solve the problems of poor anti-inflammatory effect, easy allergy, and poor acne treatment effect of common skin care products in the prior art.

[0005] One of the traditional Chinese medicine compositions in this scheme consists of 5-35 parts of prickly pear, 10-45 parts of firethorn fruit, 5-45 parts of dendrobium nobile, and 5-45 parts of artemisia annua.

[0006] The working principle and beneficial effects of this solution: The prickly pear is the fruit of *Rosaroxburghii Tratt.*, a perennial shrub belonging to the Rosaceae family. It is a medicinal and edible fruit in southwestern my country. As early as the Qing Dynasty, Zhao Xuemin's *Compendium of Materia Medica Supplement* recorded its medicinal use, stating that "the roots, stems, leaves, fruits, and seeds of the prickly pear can all be used medicinally, possessing stomach-strengthening, digestive, and nourishing effects." In ethnic minority areas of southwestern China, it is commonly used to treat indigestion, weakness and emaciation, food stagnation and bloating, metrorrhagia, and leukorrhea. Modern pharmacological research shows that it has hypoglycemic, hypolipidemic, antioxidant, antitumor, and antiviral effects. Chemical composition studies indicate that the prickly pear contains abundant vitamin C, triterpenes, flavonoids, organic acids, and other chemical components.

[0007] Firethorn berries are the fruit of the evergreen shrub *Pyracantha fortuneana* (Maxim.) HLLi, belonging to the genus *Pyracantha* of the Rosaceae family. It is named for its red berries and sharp thorns on its branches. According to the *Compendium of Materia Medica*, firethorn has high medicinal value, capable of relieving pain, promoting blood circulation, and stopping bleeding; its roots can also clear heat and cool the blood.

[0008] Dendrobium nobile Lindl., also known as Bianhuangcao or Wanzhangxu, is a plant belonging to the genus Dendrobium in the Orchidaceae family. It is a traditional and precious medicinal plant in my country, listed as a superior herb in the *Shennong Bencao Jing* (Shennong's Classic of Materia Medica) and hailed as one of the "Nine Immortal Herbs." The medicinal part is the fresh or dried stem, which has the effects of strengthening yin and replenishing essence, nourishing the stomach and intestines, and promoting longevity. Modern pharmacological studies have shown that the main active ingredients of Dendrobium nobile are alkaloids and polysaccharides, possessing multiple effects such as anti-tumor, antioxidant, anti-aging, immune enhancement, regulating gastrointestinal motility, lowering blood sugar, and lowering blood lipids. The alkaloids and polysaccharides in Dendrobium nobile also have anti-inflammatory, antibacterial, whitening, and moisturizing effects on the skin.

[0009] Blumea balsamifera DC., also known as Dafeng Ai or Bingpian Ai, and in Miao medicine as Dangwokai, is a perennial herbaceous plant belonging to the Asteraceae family, widely distributed in Guangxi, Guizhou, Yunnan, Guangdong, and other provinces (autonomous regions). It possesses the effects of dispelling wind and dampness, warming the middle jiao and stopping diarrhea, promoting blood circulation and detoxifying. As a Miao medicine, it is commonly used to treat colds due to wind-cold, headaches, rheumatic pain, cold-damp diarrhea, and injuries from falls. Besides its direct clinical use as a traditional medicine, Blumea balsamifera is also a major raw material for extracting natural borneol—Artemisia argyi (L-carnitine). Chemical composition studies have revealed that Blumea balsamifera contains various components such as volatile oils, flavonoids, sesquiterpenes, and phenolic acids.

[0010] This traditional Chinese medicine composition has good anti-inflammatory activity, can significantly improve the level of cellular inflammatory factors, and effectively solves the problems of poor anti-inflammatory effect, easy allergic reaction and poor treatment effect of common acne skin care products.

[0011] Furthermore, the traditional Chinese medicine composition consists of 5 parts prickly pear, 15 parts firethorn fruit, 25 parts dendrobium nobile, and 45 parts artemisia. Experiments have shown that this composition exhibits the strongest anti-inflammatory activity.

[0012] Furthermore, the traditional Chinese medicine composition includes one or more drugs and / or extracts for anti-inflammatory and antioxidant purposes. Anti-inflammatory drugs can relieve skin inflammation, promote wound healing, and have significant therapeutic effects on inflammatory skin diseases such as dermatitis and acne. Antioxidant drugs can resist skin aging, protect against ultraviolet damage, and improve skin texture.

[0013] Furthermore, the anti-inflammatory drugs include aspirin, salicylic acid, acetaminophen, and amoxicillin.

[0014] Furthermore, the antioxidants include vitamin C, vitamin E, resveratrol, quercetin, and dihydroquercetin.

[0015] Furthermore, according to another aspect of the present invention, the use of a traditional Chinese medicine composition in the preparation of a medicament for treating and preventing acne is provided.

[0016] Furthermore, according to another aspect of the present invention, the above-mentioned traditional Chinese medicine composition is used in various skin care products such as face masks and face creams for treating acne. Detailed Implementation

[0017] The following detailed description illustrates the specific implementation method:

[0018] Example:

[0019] The traditional Chinese medicine compositions 1-11 of this invention employ a uniform design experimental scheme: referring to the literature (Liang Maoxin, Li Dong'an. Improved application of uniform design in the selection of small compound traditional Chinese medicine formulas [J]. World Science and Technology). Modernization of Traditional Chinese Medicine, 2006, 8(02):53 56.), a uniform design scheme U11(115) was prepared (as shown in Table 1). The four Chinese medicinal materials in the formula, namely, prickly pear, firethorn fruit, dendrobium nobile, and artemisia, were used as the test factors. Each factor was tested at 11 levels (i.e., 11 different doses in a gradient). The test dose was 0. Between 50g and 50g, each factor and level was arranged according to a uniform design table to construct a formulation, resulting in 11 groups of traditional Chinese medicine compositions.

[0020] Table 1. Uniform Design Table of Traditional Chinese Medicine Composition U11(115)

[0021]

[0022] Preparation of Traditional Chinese Medicine Compositions 1-11: According to the amounts of medicinal materials in Compositions 1-11 in Table 1, weigh each medicinal material according to the prescription for each group, pulverize them into coarse powder, and mix them evenly. Add 10 times the amount of purified water to each group based on the total amount of medicinal materials, soak for 1 hour, then decoct for 1.5 hours. After cooling, filter and collect the filtrate. Add 8 times the amount of purified water to the dregs, decoct again for 1 hour, cool, filter, combine the filtrates from the two decoctions, and concentrate to twice the volume (volume / mass ratio) of the original medicinal materials. Let stand at 70℃, add 3 times the amount of 95% ethanol while hot, let stand for 4 hours to precipitate, collect the supernatant, recover the ethanol under reduced pressure, and concentrate. Dry the concentrate under reduced pressure at 60℃ to obtain the extract of Traditional Chinese Medicine Compositions 1-11.

[0023] Evaluation of the anti-inflammatory activity of traditional Chinese medicine compositions 1-11: Logarithmic growth phase Raw264.7 cells were taken and made into a single-cell suspension with a complete medium containing 10% FBS and 1% streptomycin. After adjusting the concentration to 105 cells / mL, they were inoculated into a 96-well plate and cultured for 18 hours. After the cells adhered, extracts of traditional Chinese medicine compositions 1-11 at a concentration of 1 g / mL were added respectively. After pretreatment for 5 hours, lipopolysaccharide (LPS) at a concentration of 1.0 μg / mL was added. After co-incubation for 24 hours, the 96-well plate was centrifuged at 1000×g for 10 minutes on a centrifuge, and the supernatant was taken. According to the instructions of the nitric oxide kit (Shanghai Enzyme-linked Biotechnology Co., Ltd.), the content of NO was measured. Another supernatant was taken, and according to the instructions of the enzyme-linked immunosorbent assay kit (Shanghai Enzyme-linked Biotechnology Co., Ltd.), the contents of inflammatory factors IL-6, IL-1β, and TNF-α were measured. The results are shown in Table 2.

[0024] Table 2 Effects of different compositions on NO and inflammatory factors in LPS-induced RAW264.7 cells (n = 5)

[0025]

[0026] It can be seen from the data in Table 2 that traditional Chinese medicine composition 2 (composed of 5 parts of Rosa roxburghii Tratt, 15 parts of Pyracantha fortuneana (Maxim.) Li, 25 parts of Dendrobium nobile Lindl, and 45 parts of Blumea balsamifera (L.) DC.), traditional Chinese medicine composition 5 (composed of 20 parts of Rosa roxburghii Tratt, 45 parts of Pyracantha fortuneana (Maxim.) Li, 15 parts of Dendrobium nobile Lindl, and 10 parts of Blumea balsamifera (L.) DC.), traditional Chinese medicine composition 7 (composed of 30 parts of Rosa roxburghii Tratt, 10 parts of Pyracantha fortuneana (Maxim.) Li, 45 parts of Dendrobium nobile Lindl, and 5 parts of Blumea balsamifera (L.) DC.), and traditional Chinese medicine composition 8 (composed of 35 parts of Rosa roxburghii Tratt, 20 parts of Pyracantha fortuneana (Maxim.) Li, 5 parts of Dendrobium nobile Lindl, and 30 parts of Blumea balsamifera (L.) DC.) can better inhibit the release of cellular NO and inflammatory factors, showing good anti-inflammatory activity. Especially, traditional Chinese medicine composition 2 (composed of 5 parts of Rosa roxburghii Tratt, 15 parts of Pyracantha fortuneana (Maxim.) Li, 25 parts of Dendrobium nobile Lindl, and 45 parts of Blumea balsamifera (L.) DC.) has the strongest anti-inflammatory activity.

[0027] Evaluation of the anti-inflammatory activity of traditional Chinese medicine composition 2 in animals: After 50 male ICR mice (provided by the Experimental Animal Center of Zunyi Medical University, animal production license number: SCXK(Qian)2019-0001) were adaptively fed for 1 week, they were randomly divided into 5 groups with 10 mice in each group, namely:

[0028] 1. Model group (intragastric administration of 0.5% CMC-Na solution);

[0029] 2. Positive control group (intragastric administration of 0.2 g / kg / d aspirin, Bayer Healthcare Co., Ltd.);

[0030] 3. High-dose group of composition 2 (intragastric administration of 1 g / kg / d extract of composition 2, prepared by the method of Example 1, the same below);

[0031] 4. Medium-dose group of composition 2 (0.5 g / kg / d extract of composition 2 by gavage);

[0032] 5. Low-dose group of composition 2 (0.25 g / kg / d extract of composition 2 administered by gavage).

[0033] The model group was administered 0.5% CMC-Na solution by gavage, while other groups were administered the corresponding drugs by gavage in equal volumes for five consecutive days. One hour after the last administration, xylene (Nanjing Chemical Reagent Co., Ltd.) was used to stimulate inflammation of the mouse auricles. One hour later, the mice were sacrificed, and both ears were removed along the auricle baseline. One earlobe was taken from the same location using a punch and weighed using an electronic balance. The weight of the inflamed earlobe minus the weight of the control earlobe was the degree of swelling, and the inhibition rate was calculated. The degree of swelling was the weight difference (mg) between the two earlobe groups: Inhibition rate = (Swelling degree of the model group - Swelling degree of the administered group) / Swelling degree of the model group × 100%.

[0034] The results are shown in Table 3. Compared with the model group, high, medium and low doses of the traditional Chinese medicine composition 2 can significantly inhibit xylene-induced ear swelling in mice (P<0.05) and are dose-dependent, indicating that the traditional Chinese medicine composition 2 (composed of 5 parts of prickly pear, 15 parts of firethorn fruit, 25 parts of dendrobium nobile and 45 parts of artemisia) has good anti-inflammatory activity.

[0035] Table 3. Effects of the extract of traditional Chinese medicine composition 2 on xylene-induced ear swelling in mice. ±SD)

[0036]

[0037] Note: Compared with the model group, * p<0.05； Swelling degree is the weight difference between the two ear pieces (mg);

[0038] Inhibition rate = (Swelling degree in model group - Swelling degree in treatment group) / Swelling degree in model group × 100%

[0039] Preparation of the Chinese herbal composition 2 facial mask: Facial mask formula composition: water 83.68%, Chinese herbal composition 2 extract 10.0%, glyceryl polyether-26 2.0%, 1,3-butanediol 1.0%, glycerin 2.0%, polydimethylsiloxane 0.5%, sodium hyaluronate 0.05%, acetyl hexapeptide 0.5%, disodium EDTA 0.05%, 1,2-hexanediol 0.15%, capryloyl hydroxamic acid 0.05%, PEG-40 hydrogenated castor oil 0.02%.

[0040] Preparation process: Add the prescribed amount of water, extract of Chinese herbal composition 2, glycerin, and polydimethylsiloxane to the main pot and stir and heat to 85°C to fully dissolve them; then add 1,3-butanediol, sodium hyaluronate, and disodium EDTA and continue stirring until homogeneous; cool to 45°C and add the remaining components in sequence, continue stirring slowly until homogeneous, and after passing the inspection, fill, package, inspect the finished product, and put it into storage.

[0041] Preliminary volunteer trials showed that the mask prepared with the extract of Chinese herbal composition 2 had no unpleasant odor or irritation, and had significant effects in removing acne and reducing inflammation.

Claims

1. A traditional Chinese medicine composition, characterized in that: The traditional Chinese medicine composition is selected from any of the following components: 5 parts prickly pear, 15 parts firethorn fruit, 25 parts dendrobium nobile and 45 parts artemisia argyi; 20 parts prickly pear, 45 parts firethorn fruit, 15 parts dendrobium nobile and 10 parts artemisia argyi; 30 parts prickly pear, 10 parts firethorn fruit, 45 parts dendrobium nobile and 5 parts artemisia argyi; 35 parts prickly pear, 20 parts firethorn fruit, 5 parts dendrobium nobile and 30 parts artemisia argyi.

2. The use of the traditional Chinese medicine composition according to claim 1 in the preparation of a medicament for treating and preventing acne.

3. The application of the traditional Chinese medicine composition according to claim 1 in the preparation of facial masks and creams for treating acne.

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