A traditional Chinese medicine composition for preventing influenza, and its preparation method and application

Abstract

The present invention belongs to the field of traditional Chinese medicine technology, and specifically relates to a traditional Chinese medicine composition for preventing influenza, its preparation method, and application. The composition comprises the following raw materials in parts by weight: 6-30g of raw coix seed, 5-30g of kapok flower, 3-20g of patchouli, 3-15g of perilla leaf, 3-15g of siler, 3-15g of forsythia, 3-20g of astragalus, and 5-20g of ginger. The traditional Chinese medicine composition of the present application uses authentic Lingnan medicinal materials, is simple, convenient, inexpensive, and effective, and can treat both cold and warm effects, dispel pathogenic factors without damaging the body's health, strengthen the body's health without restraining pathogenic factors, and eliminate dampness without damaging yin. It dispels pathogenic factors and strengthens the body's health simultaneously, has a good effect on preventing influenza, can enhance the body's immunity, and has a good preventive and health-care effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for preventing influenza, a preparation method thereof and an application thereof. Background Art

[0002] Monitoring data from the Chinese Center for Disease Control and Prevention show that since February 2023, the positive rate of influenza virus testing in southern and northern provinces of my country has continued to rise, with the H1N1 and H3N2 subtypes and the Victoria strain of influenza B virus being the main test results. my country adheres to the principles of infectious disease prevention and control based on "prevention first, combining prevention and treatment, scientific and legal, and classified." While there are numerous traditional Chinese and Western medicine preventive programs for influenza, few traditional Chinese medicine formulas can simultaneously achieve the effects of "eliminating pathogenic factors without harming the body's health, strengthening the body's health without restraining pathogenic factors, and transforming dampness without harming Yin." Therefore, it is crucial to develop a traditional Chinese medicine formula that can achieve these aforementioned effects. Summary of the Invention

[0003] The purpose of the present invention is to provide a traditional Chinese medicine composition for preventing influenza and its preparation method and application, so as to solve the above problems.

[0004] According to one aspect of the present invention, the present invention provides a traditional Chinese medicine composition for preventing influenza, which comprises the following raw medicines in parts by weight: 6-30 parts of raw coix seed, 5-30 parts of kapok flower, 3-20 parts of patchouli, 3-15 parts of perilla leaf, 3-15 parts of siler, 3-15 parts of forsythia, 3-20 parts of astragalus, and 5-20 parts of ginger.

[0005] In this formula, Forsythia suspensa is bitter and slightly cold, clearing heat and detoxifying, dispersing wind-heat; Saposhnikovia divaricata is pungent, sweet, and slightly warm, dispersing wind and relieving exterior symptoms, serving as a lubricant in wind-clearing medicines. Together, these two are the main herbs, working together to clear heat and detoxify, dispel pathogenic factors and ward off filth. Raw Coix lachryma-jobi seeds are sweet, mild, and cooling, strengthening the spleen and stomach, tonifying the lungs, clearing heat, and dispelling wind and dampness. Kapok flowers clear heat and dampness, relieving depression and relieving restlessness. Patchouli is pungent and slightly warm, dispersing exterior symptoms, dispersing pathogenic factors, dispelling dampness and wind, and clearing heat and quenching thirst. Together, these three are the assistant herbs, assisting the main herbs in dispelling pathogenic factors and detoxifying, while also invigorating the spleen and stomach and clearing dampness and turbidity. Astragalus is a sacred Qi-tonifying herb. Combined with Forsythia suspensa, it clears heat and detoxifies without damaging Qi. Combined with Saposhnikovia divaricata, it dispels wind and resolves exterior symptoms with a powerful, sturdy effect. Perilla leaf, pungent and warm, dispels exterior symptoms and dispels cold, promotes Qi circulation and alleviates fullness. Combined with Saposhnikovia divaricata, it enhances its exterior-clearing properties. Combined with raw Coix seed, Kapok flower, and Patchouli, it strengthens its spleen-tonifying and dampness-removing properties. Astragalus and Perilla leaf serve as adjuvants. Ginger, pungent and warm, enters the lungs, spleen, and stomach, dispelling exterior symptoms and dispelling cold, warming the middle and relieving vomiting, and warming the lungs and relieving coughs. It harmonizes the other herbs and serves as a guiding herb. Together, these herbs achieve the effects of clearing heat and dispersing wind, expelling pathogenic factors, and dispelling pathogenic factors.

[0006] The Chinese medicine composition of this application, through the rational combination of multiple Chinese herbs and their synergistic effects, not only clears away heat and dispels wind, expel pathogenic pathogens, but also strengthens the spleen and eliminates dampness, invigorates the spleen and harmonizes the stomach, and can specifically relieve discomfort such as nasal congestion and runny nose, fatigue, dry and sore throat, poor appetite and nausea, and loose stools. Guided by the academic theories of "three causes, three treatments," "prevent illness before it occurs," and "maintaining vital energy within to prevent pathogenic pathogens from entering," Traditional Chinese Medicine (TCM) can simultaneously act on multiple biological pathways to exert a broad-spectrum antiviral effect, making it simple, convenient, inexpensive, and effective in preventing and treating influenza.

[0007] The Chinese medicine composition of the present application uses authentic Lingnan medicinal materials, uses both cold and warm properties, dispels evil without harming the body, strengthens the body without restraining evil, transforms dampness without harming yin, and dispels evil and strengthens the body at the same time. It has a good effect on preventing influenza, can enhance the body's immunity, and plays a very good preventive and health-care effect.

[0008] In some embodiments, the proportion of the raw materials of the above-mentioned traditional Chinese medicine composition can be preferably: 10-25 parts of raw coix seed, 10-25 parts of kapok flower, 5-15 parts of patchouli, 5-15 parts of perilla leaf, 5-15 parts of siler, 5-15 parts of forsythia, 5-15 parts of astragalus, and 10-20 parts of ginger.

[0009] In some embodiments, the proportion of the raw materials of the above-mentioned traditional Chinese medicine composition can be further preferably: 20 parts of raw coix seed, 15 parts of kapok flower, 10 parts of patchouli, 10 parts of perilla leaf, 10 parts of siler, 10 parts of forsythia, 10 parts of astragalus, and 15 parts of ginger.

[0010] In some embodiments, the above-mentioned Chinese medicine composition may also include medically acceptable excipients to prepare different dosage forms required by patients, which are convenient for use, storage and transportation, and meet clinical and market needs; the excipients used are all excipients commonly used in the prior art, including excipients, flavoring agents, adhesives, fillers, disintegrants, lubricants, etc.

[0011] In some embodiments, the Chinese medicine composition for preventing influenza can be in the form of pills, tablets, capsules, granules, oral solutions, powders or sprays, etc. Among them, the Chinese medicine composition is often taken in the form of oral solutions, which is convenient for patients to prepare and take.

[0012] According to another aspect of the present invention, the present invention also provides a method for preparing a traditional Chinese medicine composition for preventing influenza. When the traditional Chinese medicine composition is an oral solution, the preparation method comprises the following steps:

[0013] (1) Weigh the raw materials according to the ratio;

[0014] (2) adding the raw materials into 8-12 parts by weight of water for soaking, then heating to boiling and maintaining a slight boiling, and separating to obtain a first decoction and medicinal residue;

[0015] (3) Take the residue obtained after the first decoction, add water equivalent to 8-10 times the weight of the total mass of the raw material, heat to boiling, keep it slightly boiling, and obtain the second decoction and the residue after separation; combine the first decoction and the second decoction to obtain an oral solution.

[0016] According to another aspect of the present invention, the present invention also provides a method for preparing a traditional Chinese medicine composition for preventing influenza. When the traditional Chinese medicine composition is a powder, the preparation method comprises the following steps:

[0017] (1) Take raw coix seeds, crush them, and pass them through 10-mesh and 50-mesh sieves at the same time. Continue to crush the coarse particles that cannot pass through the 10-mesh sieve, and take the powder between the 10-mesh sieve and the 50-mesh sieve;

[0018] (2) crushing kapok flowers, patchouli, perilla leaves, siler leaves, forsythia, astragalus, and ginger into powder according to the method of step (1);

[0019] (3) Mix all the raw material powders evenly, put them into a packaging bag, and seal it.

[0020] According to another aspect of the present invention, there is provided a use of the above-mentioned traditional Chinese medicine composition for preventing influenza in the preparation of a drug for preventing influenza.

[0021] The traditional Chinese medicine composition for preventing influenza of the present invention can also be used to prepare medicines for relieving symptoms of nasal congestion and runny nose, fatigue, dry and sore throat, poor appetite and nausea, and loose stools. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Figure 3 shows the weight change trends of mice in different groups in the death protection experiment; NC represents the blank group, V represents the virus group, YH represents the high-dose group of the traditional Chinese medicine composition (concentration is 6.072 g / ml), YM represents the medium-dose group of the traditional Chinese medicine composition (concentration is 3.036 g / ml), and YL represents the low-dose group of the traditional Chinese medicine composition (concentration is 1.518 g / ml).

[0023] Figure 2 、 Figure 3 These are the survival curves of each group under different situations in the death protection experiment.

[0024] Figure 4 This is the trend of body weight changes in different groups of mice in the acute lung injury protection experiment in mice.

[0025] Figure 5 Figure 3 shows the lung tissue structure and HE staining of mice in different groups.

[0026] Figure 6 Figure 2 shows the expression of H1N1 mRNA in lung tissues of mice in different groups.

[0027] Figure 7The lung index of mice in different groups. DETAILED DESCRIPTION

[0028] The present invention will be further described in detail below in conjunction with specific embodiments. Unless otherwise specified, the following raw materials are all commercially available.

[0029] The Chinese medicine composition provided by the present invention is mainly aimed at preventing influenza. The components and weights of Examples 1-2 of the present invention are listed below.

[0030] Example 1

[0031] A traditional Chinese medicine composition for preventing influenza, comprising the following raw medicinal materials: 20g of raw coix seed, 15g of kapok flower, 10g of patchouli, 10g of perilla leaf, 10g of siler, 10g of forsythia, 10g of astragalus, and 15g of ginger.

[0032] When the above-mentioned Chinese medicine composition is prepared into an oral solution, it can be prepared according to the following method:

[0033] (1) Weigh approximately 20g of raw coix seed, 15g of kapok flower, 10g of patchouli, 10g of perilla leaf, 10g of siler, 10g of forsythia, 10g of astragalus, and 15g of ginger;

[0034] (2) Add the weighed raw drug substance to water of about 10 times its weight and soak for about 30 minutes, then heat to boiling and keep it slightly boiling for about 1 hour, and separate to obtain a first decoction and a medicinal residue; take the medicinal residue obtained after the first decoction, add water of about 8 times its weight to the total mass of the raw drug substance, heat to boiling, keep it slightly boiling for about 1 hour, and separate to obtain a second decoction and a medicinal residue; combine the first decoction and the second decoction to obtain an oral solution;

[0035] (3) The obtained oral solution is cooled and then divided into portions, the proportion of which is one dose, and one dose is about 150 mL.

[0036] Example 2

[0037] A traditional Chinese medicine composition for preventing influenza, comprising the following raw medicinal materials: 20g of raw coix seed, 15g of kapok flower, 10g of patchouli, 10g of perilla leaf, 10g of siler, 10g of forsythia, 10g of astragalus, and 15g of ginger.

[0038] It can also be ground into powder and made into decoction or granule dosage forms according to the following preparation method:

[0039] (1) Take raw coix seeds, crush them, and pass them through 10-mesh and 50-mesh sieves at the same time. Continue to crush the coarse particles that cannot pass through the 10-mesh sieve, and take the powder between the 10-mesh sieve and the 50-mesh sieve;

[0040] (2) crushing kapok flowers, patchouli, perilla leaves, siler leaves, forsythia, astragalus, and ginger into powder according to the method of step (1);

[0041] (3) Mix all the raw material powders evenly, put them into a packaging bag, and seal it.

[0042] To illustrate the therapeutic effect of the Chinese medicine composition of the present invention, the following animal experiment was conducted: wherein the Chinese medicine composition used was the oral solution prepared in Example 1.

[0043] 1. Death Protection Experiment

[0044] Fifty 6-8-week-old Balb / c mice were divided into five groups (10 male mice each): a blank group, a virus group, a high-dose Chinese medicine composition group, a medium-dose Chinese medicine composition group, and a low-dose Chinese medicine composition group. All but the blank group were infected intranasally with 2 LD50 of influenza A virus (H1N1 / A / PR8) virus dilution. The Chinese medicine composition group was administered intragastrically, starting one week before challenge, once daily for seven days. Body weight and general condition of the mice were observed and recorded daily, with observation ending on day 14 post-infection. The mortality protection rate was calculated, as shown in Tables 1-2.

[0045] Table 1 Actual death rate

[0046]

[0047] Note: The actual death rate was calculated and the log-rank test was used to compare the survival time of different groups. The survival curves of the virus group and the high-dose group of the preventive prescription showed significant differences (P < 0.05), and the survival curves of the virus group and the low-dose group showed significant differences (P < 0.05).

[0048] Table 2 Cases where body weight < 70% is considered death

[0049]

[0050] Note: According to animal ethics requirements, 70% of body weight was used as the humane endpoint to calculate the mortality rate. The log-rank test was used to compare the survival time of different groups. There was no significant difference in the survival curves between the virus group and the high, medium and low dose groups of the preventive prescription (P>0.05).

[0051] 2. Protection Experiment on Acute Lung Injury in Mice

[0052] Sixty 6-8 week old Balb / c mice were divided into 8 groups, 12 male mice each, including a blank group, a model group, a high-dose Chinese medicine composition group, a medium-dose Chinese medicine composition group, a low-dose Chinese medicine composition group, a honeysuckle group, a ginger group, and a honeysuckle + ginger group. All mice, except the blank group, were infected intranasally with 2LD50 of a virus dilution of influenza A virus (H1N1 / A / PR8). The Chinese medicine composition group was gavage-administered once daily starting one week before the challenge, and continued until the end of the sample collection. The honeysuckle group, ginger group, and honeysuckle + ginger group were administered with the drug for 7 consecutive days after the intranasal challenge. The body weight and general condition of the mice were observed and recorded daily. On the 7th day after infection, blood and lung tissue were collected from the mice after an intraperitoneal injection of 100 mg / kg sodium pentobarbital.

[0053] Among them, the honeysuckle group received 30g of honeysuckle, and the small yellow ginger group received 60g of small yellow ginger; the honeysuckle + small yellow ginger group: 30g of honeysuckle + 60g of small yellow ginger.

[0054] Observation indicators:

[0055] 1. All BALB / c mouse lung tissues were fixed with formaldehyde and then stained with HE to observe the pathological sections.

[0056] 2. All BALB / c mouse lung tissues were collected to calculate the mouse lung index.

[0057] 3. Lung tissues of 6 BALB / c mice in each group were subjected to qPCR experiments to observe viral replication.

[0058] The weight changes of experimental mice in each group can be found in Figure 4 Compared with the model group, the Chinese medicine combination group can improve the weight loss of mice, suggesting that the Chinese medicine combination group may improve the survival rate of mice.

[0059] All BALB / c mouse lung tissues and their HE staining images can be found in Figure 5 The results showed that the lung tissue structure of mice in the normal control group (i.e., blank group) was normal, with thin alveolar walls, clearly visible alveolar cavities, no bleeding and inflammatory cell infiltration. The alveolar walls and septa of mice in the model control group were thickened, with inflammatory cell infiltration and more obvious damage. Compared with the normal control group, the degree of lung pathological damage in the model control group was significantly increased, indicating that the model was successful. Compared with the model control group, the pathological degree of lung tissue in the high-, medium-, and low-dose groups of the Chinese medicine composition, the honeysuckle group, the ginger group, and the honeysuckle + ginger group was significantly reduced, the lung morphology and structure were relatively normal, the degree of inflammatory cell infiltration was significantly reduced, and there was basically no pathological damage. It can be inferred that the Chinese medicine composition has a certain protective effect on acute lung injury.

[0060] Figure 6 and Figure 7The results showed that compared with the normal control group mice, the expression of H1N1 mRNA and lung index in the model group mice's lung tissue were significantly increased (P<0.05); compared with the model control group mice, the expression of H1N1 mRNA in the three groups of high, medium and low doses of the Chinese medicine composition and the honeysuckle, ginger, and honeysuckle + ginger groups were significantly decreased (P<0.05), the lung index of the low dose group of the Chinese medicine composition had no significant difference from that of the model group, and the lung index of the other groups were significantly decreased (P<0.05).

[0061] The above are only some embodiments of the present invention. For those skilled in the art, several modifications and improvements can be made without departing from the inventive concept of the present invention, which all fall within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for preventing influenza A, characterized in that: The invention is composed of the following raw medicines in parts by weight: 6-30 parts of raw coix seeds, 5-30 parts of kapok flowers, 3-20 parts of patchouli, 3-15 parts of perilla leaves, 3-15 parts of siler leaves, 3-15 parts of forsythia, 3-20 parts of astragalus and 5-20 parts of ginger.

2. The Chinese medicine composition for preventing influenza A according to claim 1, characterized in that The invention is composed of the following raw medicines in parts by weight: 10-25 parts of raw coix seeds, 10-25 parts of kapok flowers, 5-15 parts of patchouli, 5-15 parts of perilla leaves, 5-15 parts of siler leaves, 5-15 parts of forsythia, 5-15 parts of astragalus and 10-20 parts of ginger.

3. The Chinese medicine composition for preventing influenza A according to claim 1, characterized in that The invention is composed of the following raw medicines in parts by weight: 20 parts of raw coix seeds, 15 parts of kapok flowers, 10 parts of patchouli, 10 parts of perilla leaves, 10 parts of siler leaves, 10 parts of forsythia, 10 parts of astragalus and 15 parts of ginger.

4. The Chinese medicine composition for preventing influenza A according to any one of claims 1 to 3, characterized in that Also included are medically acceptable excipients.

5. The Chinese medicine composition for preventing influenza A according to claim 4, characterized in that The traditional Chinese medicine composition is in the form of pills, tablets, capsules, granules, oral solutions, powders or sprays.

6. The method for preparing the traditional Chinese medicine composition for preventing influenza A according to claim 5, characterized in that: When the Chinese medicine composition is an oral solution, the preparation method comprises the following steps: (1) Weigh the raw materials according to the ratio; (2) adding the raw materials into 8-12 parts by weight of water for soaking, then heating to boiling and maintaining a slight boiling, and separating to obtain a first decoction and medicinal residue; (3) Take the residue obtained after the first decoction, add water equivalent to 8-10 times the weight of the total mass of the raw material, heat to boiling, keep it slightly boiling, and obtain the second decoction and the residue after separation; combine the first decoction and the second decoction to obtain an oral solution.

7. The method for preparing the Chinese medicine composition for preventing influenza A according to claim 5, wherein: When the Chinese medicine composition is an oral solution, the preparation method comprises the following steps: (1) Take raw coix seeds, crush them, and pass them through 10-mesh and 50-mesh sieves at the same time. Continue to crush the coarse particles that cannot pass through the 10-mesh sieve, and take the powder between the 10-mesh sieve and the 50-mesh sieve; (2) Grind kapok flowers, patchouli, perilla leaves, siler leaves, forsythia, astragalus, and ginger into powder according to the method of step (1); (3) Mix all the raw material powders evenly, put them into the packaging bag and seal it.

8. Use of the traditional Chinese medicine composition for preventing influenza A according to any one of claims 1 to 4 in the preparation of a medicament for preventing influenza A.

Images