A composition for killing dust mites or human demodex and its application

Abstract

The present invention relates to a composition for killing dust mites or human demodex, wherein the composition comprises an active ingredient for killing dust mites or human demodex, and the active ingredient comprises a mixture of an acid-type sophorolipid and a lactone-type sophorolipid. The combined mass ratio of the acid-type sophorolipid and the lactone-type sophorolipid is 99-10:1-90. The present invention adopts a combination of an acid-type sophorolipid and a lactone-type sophorolipid to obtain a composition that can efficiently kill human demodex and dust mites. Compared with a single-component acid-type sophorolipid or a lactone-type sophorolipid, the mixed sophorolipid composition has a significantly improved killing effect on human demodex and dust mites. By using the composition in daily cleaning products, not only the daily cleaning products are given the effects of decontamination, degreasing, and antibacterial, but also the dust mites and human demodex can be effectively killed, reducing the troubles caused by dust mites or human demodex to people's lives.

Description

Technical Field

[0001] The invention relates to the technical field of daily cleaning and care, and in particular to a composition for killing dust mites or human demodex mites and an application thereof. Background Art

[0002] Mites are a general term for all species in the subclass Acari except ticks, with about 40,000 species belonging to more than 400 categories. The main ones closely related to human life are dust mites and human Demodex. Among them, dust mites are the most common indoor allergens. Their secretions, excretions and tissue fragments can induce allergic rhinitis, asthma, rashes and other allergic diseases. Due to the rapid reproduction rate and astonishing number of dust mites and their strong pathogenicity, accidentally eating food contaminated by mites can cause severe allergic reactions. Human Demodex is a parasitic mite that mostly parasitizes in human hair follicles and sebaceous glands. The clinical manifestations of facial diseases caused by Demodex infection include acne, rosacea, enlarged pores, blackheads, orange peel-like changes in the skin, and skin flushing.

[0003] At present, there are biological miticides and chemical miticides for the prevention and control of mites. However, due to safety issues, effective miticide prevention and control methods are mostly concentrated in the field of animal breeding and applied to livestock and other animals. Currently, commonly used chemical miticides include benzyl benzoate, lindane, chlorfenapyr, permethrin, malathion and sulfur. However, the non-degradability of chemical miticides brings about chemical drug residues, environmental hazards and drug resistance, which makes their use in mite prevention and control more restricted. Biological miticides include avermectin. Avermectin produced by Streptomyces can effectively kill mites on the surface and inside the body of animals, but its high-dose use leads to high antibiotic residues in livestock products, which has aroused strong concern among consumers. Therefore, it is very meaningful to develop some miticide products that are safe, mild, non-irritating and non-toxic to humans.

[0004] However, there is still a lack of safe and mild acaricides for humans and mature technology in the field of daily chemicals. Most of the common ones are plant extracts. For example, patent application CN112043817A discloses an anti-mite composition, whose anti-mite components are 1-10 parts of antimicrobial peptides, 1-10 parts of lilac extracts and 1-10 parts of Artemisia annua extracts. The killing rate of rabbit mites with one medication is 83%, but the killing rate of dust mites and human demodex mites has not been tested, and the components that really play a mite-killing role are not clear. For another example, patent application CN106821844A discloses an anti-mite composition, which contains 5-10 parts of Phellodendron chinense extract, 20-30 parts of mung bean powder, 5-15 parts of grape seed extract, and 40-50 parts of deep sea mud. This scheme only verifies that it has a certain alleviating effect on the symptoms of mite dermatitis, and does not directly confirm whether it has a killing effect on dust mites or human demodex mites. In addition, some research reports mentioned that plant essential oils have acaricidal effects. For example, the research by Jiang Liyan (2009) and others showed that Zanthoxylum bungeanum volatile oil has a significant inhibitory and killing effect on human Demodex mites. In terms of killing dust mites, it has been reported that plant essential oils such as fennel, Korean celery, clove, pine red plum, atractylodes, peony, cinnamon, asarum, patchouli, and cypress have significant killing effects. These have provided more possible choices for the acaricidal effects of daily chemical products. However, the characteristic odor of plant essential oils and the fact that they can easily cause skin redness and swelling have limited the number of product recipients to a certain extent. At the same time, the unclear active ingredients of plant extracts also make it impossible to stably control their acaricidal effects. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a composition for killing dust mites or human Demodex and its application. The glycolipid composition is a composition of a lactone-type sophorolipid and an acid-type sophorolipid. The composition not only exhibits excellent activity in killing dust mites or human Demodex, but also has the emulsification, dispersion, washing, solubilization and other functions of a surfactant. It can be used in various toiletries to give the product excellent efficacy in killing dust mites or human Demodex.

[0007] (II) Technical solution

[0008] The present invention relates to a mixed sophorolipid composition and its application. In addition to the hydrophilic / lipophilic surfactant functions of conventional sophorolipids, the mixed sophorolipid composition also has excellent bactericidal and mite removal functions. The mixed sophorolipid composition is applied to different types of daily chemical washing, skin care, and indoor purification products, thereby obtaining a product that has affinity with human skin and can effectively kill dust mites or human demodex mites that are closely related to human life, and has the characteristics of safety, mildness, no irritation, and no toxic residue.

[0009] The technical solutions of the present invention include the following:

[0010] In a first aspect, the present invention provides a composition for killing dust mites or human Demodex, wherein the composition comprises an active ingredient for killing dust mites or human Demodex, and the active ingredient comprises a mixture of an acid-type sophorolipid and a lactone-type sophorolipid.

[0011] According to a preferred embodiment of the present invention, the active ingredients of the composition consist only of acid-type sophorolipids and lactone-type sophorolipids, that is, the composition uses the sophorolipid composition composed of acid-type sophorolipids and lactone-type sophorolipids as the only active ingredient.

[0012] According to a preferred embodiment of the present invention, in the active ingredient, the combined mass ratio of acid-type sophorolipids and lactone-type sophorolipids is 99-10:1-90; preferably, the combined mass ratio of acid-type sophorolipids and lactone-type sophorolipids is 10-90:90-10; more preferably, the combined mass ratio of acid-type sophorolipids and lactone-type sophorolipids is 10:90. In the aforementioned combined mass ratio, the sum of the ratios of acid-type sophorolipids and lactone-type sophorolipids is 100.

[0013] According to a preferred embodiment of the present invention, in the composition, the sophorolipid composition composed of acid-type sophorolipid and lactone-type sophorolipid accounts for 20-90% of the total mass of the composition, preferably 30-70%, more preferably 40-60%, and further preferably 55%.

[0014] According to a preferred embodiment of the present invention, the composition further comprises a solvent, and the solvent is at least one of water, ethanol, glycerol, propylene glycol, butylene glycol, pentanediol, ethyl acetate and ethyl propionate. Preferably, the solvent is at least one of water, ethanol and glycerol; the solvent accounts for 10-80% of the total mass of the composition, preferably 30-70%, and more preferably 40-60%.

[0015] According to a preferred embodiment of the present invention, the composition further comprises a colorant or a fragrance.

[0016] According to a preferred embodiment of the present invention, the composition consists only of acid-type sophorolipids and lactone-type sophorolipids, and does not contain other ingredients except the active ingredient.

[0017] The present invention produces and sells the composition for killing dust mites or human demodex as a separate product, and adds it into daily cleaning products that are close to people's lives in the form of additives.

[0018] In a second aspect, the present invention also relates to the application of the above-mentioned composition for killing dust mites or human demodex, which includes: adding the composition for killing dust mites or human demodex as an additive to daily cleaning products; or directly diluting it to make a dust mite or human demodex killing agent.

[0019] According to a preferred embodiment of the present invention, the daily cleaning products include but are not limited to the following products: household cleaning agents (laundry detergents, dishwashing agents, fruit and vegetable cleaning agents, washing powders, washing machine cleaning agents, collar cleaners, down coat care agents, tile cleaning agents), cleaning agents (shampoo, hand soap, hair care liquid, hair mask, facial cleanser, soap, toothpaste, mouthwash, oral freshening spray, face cream, lotion, essence, facial mask, toner, shower gel, makeup remover, skin cleanser, makeup, perfume, wet wipes), air purifiers, bedding mite removers, underwear mite removers, furniture cleaners, furniture care agents, kitchen cleaners, mosquito repellents, bathroom cleaners, glass cleaners, carpet cleaners and other daily chemicals. By adding the composition to the aforementioned products to replace conventional synthetic surfactants, it not only has the functions of surfactants, but also has antibacterial and antiseptic properties. More importantly, it can give the product a mild, non-irritating, non-allergic effect of killing dust mites or human demodex.

[0020] According to a preferred embodiment of the present invention, in a daily cleaning product, the amount of the composition for killing dust mites or human demodex is as follows: the mass percentage of the sophorolipid composition composed of acid-type sophorolipids and lactone-type sophorolipids in the daily cleaning product is 0.1-20%, preferably 0.5-10%, and more preferably 1-5%. The concentration of the sophorolipid composition should meet the above concentration, and within the above concentration range, an effective killing rate of human demodex or dust mites can be exerted.

[0021] In order to prepare the composition for killing dust mites or human demodex, it is necessary to combine two sophorolipids with different chemical structures. Specifically, the preparation method can be: respectively measure a certain mass of acid-type sophorolipid and lactone-type sophorolipid, and then stir and mix. Then, add at least one of some solvents, toners and fragrances as needed to obtain the composition product for killing dust mites or human demodex. The mass ratio of acid-type sophorolipid and lactone-type sophorolipid can be designed as needed.

[0022] (III) Beneficial effects

[0023] The present invention combines acid-type sophorolipids and lactone-type sophorolipids in a certain proportion, and can efficiently kill mites closely related to human life, such as human demodex and dust mites. Compared with the acid-type sophorolipids of a single component, the combination of two sophorolipids significantly improves the killing effect of human demodex and dust mites, and the two sophorolipids have a synergistic effect in killing dust mites and human demodex. Therefore, the present invention adds the composition of two sophorolipids as an additive to daily cleaning products, which can not only play the emulsification, dispersion, washing, solubilization, sterilization and preservation functions of sophorolipid biosurfactants, but also give the product the function of removing dust mites and human demodex. Alternatively, the composition of the two sophorolipids can be directly diluted with a solubilizer to make a dust mite or human demodex remover for use and sale.

[0024] The sophorolipid composition of the present invention has no special smell (the taste is similar to that of sweeteners but without special smell), is non-toxic, mild, safe and non-irritating, does not cause skin redness or swelling and pain, is easily biodegradable, and can be well miscible with other organic and inorganic components. Compared with plant essential oils or plant extracts, the composition of the present invention has a wider range of applications and product acceptance. DETAILED DESCRIPTION

[0025] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below in conjunction with specific implementation methods.

[0026] Sophorolipids are bio-based surfactants produced by microorganisms through fermentation using sugar and oil as raw materials. The molecular structure consists of a molecule of long-chain fatty acid and two molecules of glucose. According to the connection form of the carboxyl group at the end of the fatty acid chain, they are divided into acid-type sophorolipids and lactone-type sophorolipids. Acid-type sophorolipids and lactone-type sophorolipids are the two main types of sophorolipids, and the difference between them lies in the different chemical bonds in their molecular structures. The characteristic of lactone-type sophorolipids is that there are one or more esterified ring structures inside its molecules, that is, a lactone ring is formed at a specific position (such as the 4' hydroxyl group and the hydroxyl or carboxyl group on another carbon atom). The structure of acid-type sophorolipids does not contain a lactone ring, but exists in the form of a free carboxyl group, which makes them have better solubility and foam formation ability. Because acid-type sophorolipids do not have the characteristics of lactonization, their physical and chemical properties are different from those of lactone types.

[0027] Due to its excellent hydrophilic and lipophilic properties and biodegradability, sophorolipids have great application potential in the fields of daily chemicals, textiles, environmental governance, agriculture, medical treatment and petrochemicals. In the field of daily chemical cleaning, the prior art mainly develops various cleaning products based on the safety, mildness and biodegradability of sophorolipid biosurfactants. Patents such as EP0499434, EP1445302, EP1411111, CN104125821 all describe the application of surfactant properties based on acid-type sophorolipids in various cleaning products. In addition, sophorolipids are also a very effective biocide, and there are many research literature reports on the bactericidal effect of sophorolipids. However, there are few studies on the insecticidal effects of sophorolipids. Patent US2023 / 0240294A1 discloses the killing effect of sophorolipids on Mesostigmata mites such as Dermanyssus gallinae, Ornithonyssus bursa, and Ornithonyssus sylviarum, and based on this effect, discloses a sophorolipid application system for controlling mites in poultry houses in the breeding industry. The sophorolipid content of the application system is 1.5-40%, and the spraying amount per 1,000 chickens in a chicken house is 2.5-20 liters.

[0028] In the present application, dust mites are a kind of micro-arthropods belonging to the family Acaridae and the genus Dust Mite. They are tiny in size, usually between 0.1 and 0.6 mm in length, difficult to detect with the naked eye, and can only be seen with the aid of a microscope. Dust mites are widely distributed in human living environments, and especially prefer warm and humid environments (temperature is about 25°C and relative humidity is about 75%), which are conducive to their reproduction and growth. Dust mites produce a large amount of excrement and body fragments during their life cycle. These particles become an important source of allergens when suspended in the air, which can cause allergic reactions in people, such as allergic rhinitis, asthma, and skin diseases. Human Demodex, also known as Demodex or Sebaceous Demodex, is a tiny parasite belonging to the class Arachnida, the order Sarcoptes, and the family Demodex. Demodex may cause chronic inflammatory reactions, thereby causing skin diseases, clinically manifested as symptoms such as erythema, papules, itching, and desquamation. This condition is collectively referred to as demodicosis or Demodex dermatitis.

[0029] Dust mites and human demodex are mites closely related to human life. They often parasitize directly on the surface of human skin, underwear, bedding, etc., so that human skin is often troubled by dust mites and human demodex, causing various skin problems. However, so far, there is no prior art that discloses the use of acid-type sophorolipids and lactone-type sophorolipids in combination to kill dust mites or human demodex. This application proposes for the first time to use acid-type sophorolipids and lactone-type sophorolipids as an active ingredient for killing dust mites or human demodex, and it can be used as the only active ingredient in the product to effectively kill dust mites or human demodex.

[0030] In the following examples, the test method for the killing effect of human Demodex is as follows:

[0031] 1.1 Experimental Materials

[0032] Equipment and reagents: biological microscope, transparent tape, slides, pipette electronic balance, RPMI1640 cell culture medium, physiological saline, latex gloves, etc.

[0033] Human Demodex: Live dust mites and human Demodex were collected from the face of infected individuals using the transparent tape method, respectively.

[0034] 1.2 Experimental methods

[0035] 1.2.1 Acquisition of experimental mite species: Instruct volunteers to clean their faces thoroughly before going to bed, and apply 6 cm long and 1.5 cm wide transparent tape to the forehead, cheeks, nose, chin and other parts of the volunteers, and press gently to make the tape fully contact the face. The next day, peel it off and stick it on a glass slide for microscopic examination. The seized human Demodex mites were stored in RPMI 1640 cell culture medium and placed in a 4°C refrigerator for later use.

[0036] a. Weigh 67 mg of the sample to be tested and place it on the slide. At the same time, take 50 μL of RPMI 1640 cell culture medium and place it on the slide. Use a bamboo stick to mix the two thoroughly and then use a pusher to spread them evenly.

[0037] b. Stick the tape with the Demodex mite on the glass slide containing the sample to be tested to ensure that the sample is in full contact with the mite. If the scraping method is used for sampling, mix the scraping material, the sample to be tested, and the RPMI 1640 cell culture medium on the glass slide. Add 200 μL of saline on another glass slide as a blank control.

[0038] c. Place the sample slide and the blank control slide in an artificial climate chamber (temperature 26°C, humidity 90%), or place a ball of wet cotton in a sealed box to make a wet box, and place the wet box in a 26°C incubator. d. Count the number of Demodex mites killed under a microscope after 4 hours, 8 hours, and 12 hours.

[0039] Twenty Demodex mites were counted in each group of experiments, and the experiment was repeated 3 times, and the results were averaged.

[0040] 1.3 Death judgment standard: Observe continuously for 1 minute under a 400x microscope. If the chelicerae, feet and claws do not move, it is preliminarily judged as dead. Observe every 5 minutes within 30 minutes. If there is still no movement, it is considered dead.

[0041] 1.4 Calculation of human demodex killing rate (calculation result is rounded to 2 decimal places):

[0042] P = [(AB) / A] * 100%;

[0043] Where: P = corrected mortality rate (%); A = number of surviving mites in the blank control group (individuals); B = number of surviving mites in the test group (individuals).

[0044] In the following examples, the test method for the killing effect on dust mites is the same as the above method, and the only difference is that the dust mites are obtained from different sources. The dust mite acquisition method is: a towel that has been hung in the bathroom for a long time without sunlight is attached to the back of the towel with a 6 cm long and 1.5 cm wide transparent tape, and gently pressed. After peeling it off, it is attached to a glass slide for microscopic examination. The seized dust mites are stored in RPMI 1640 cell culture medium and placed in a 4°C refrigerator for use.

[0045] Example 1

[0046] Acid-type sophorolipid and lactone-type sophorolipid were combined in proportion to obtain a sophorolipid composition 1# with a sophorolipid mass percentage concentration of 50%, wherein the mass ratio of acid-type sophorolipid to lactone-type sophorolipid was 10:90.

[0047] Sophorolipid composition 1# was diluted with deionized water to obtain aqueous solutions with a total concentration of 1% (50 times dilution) and 1.5% (33.3 times dilution) of sophorolipids, respectively. The plate count method was used to test the growth inhibition effects of sophorolipid aqueous solutions on Escherichia coli, Staphylococcus aureus, and Streptococcus mutans at two concentrations. The results showed that the aqueous solution with a total concentration of 1% of sophorolipids had a growth inhibition rate of 90% on Escherichia coli, and the inhibition rates on Staphylococcus aureus and Streptococcus mutans were both 100%. The aqueous solution with a total concentration of 1.5% of sophorolipids had a growth inhibition rate of 96.8% on Escherichia coli, and a 100% inhibition rate on Staphylococcus aureus and Streptococcus mutans, which was completely inhibited.

[0048] Sophorolipid composition 1# was diluted with deionized water to obtain aqueous solutions with total concentrations of 1% (50 times dilution), 0.5% (100 times dilution) and 1.5% (33.3 times dilution) of sophorolipids, respectively. The aqueous sophorolipid solutions were treated at room temperature for 30 minutes, and the killing rates of the aqueous sophorolipid solutions against dust mites and human demodex were tested at two concentrations. The results showed that the killing rates of the aqueous solution with a total concentration of 1% of sophorolipids against dust mites and human demodex were 84.7% and 98.4%, respectively; the killing rates of the aqueous solution with a total concentration of 0.5% of sophorolipids against dust mites and human demodex were 71.5% and 88.6%. The killing rates of the aqueous solution with a total concentration of 1.5% of sophorolipids against dust mites and human demodex were 94.6% and 100%.

[0049] Example 2

[0050] The acid-type sophorolipid and the lactone-type sophorolipid were mixed to obtain a sophorolipid composition 2#, wherein the mass ratio of the acid-type sophorolipid to the lactone-type sophorolipid was 40:60, and the total mass concentration of the sophorolipid in the sophorolipid composition 2# was 50%.

[0051] The sophorolipid composition 2# was diluted to obtain aqueous solutions with concentrations of 1.5% (diluted 33.3 times) and 2% (diluted 25 times), and the plate count method was used to test the growth inhibition effects on Escherichia coli and Staphylococcus aureus. The plate count method was used to test the growth inhibition effects of the sophorolipid aqueous solutions on Escherichia coli, Staphylococcus aureus, and Streptococcus mutans at two concentrations. The results showed that the aqueous solution with a total concentration of 1.5% sophorolipids had an inhibition rate of 85% on the growth of Escherichia coli, and the inhibition rates on Staphylococcus aureus and Streptococcus mutans were 100% and 98%. The aqueous solution with a total concentration of 2% sophorolipids had an inhibition rate of 100% on the growth of Escherichia coli, Staphylococcus aureus, and Streptococcus mutans, which was completely inhibited.

[0052] Sophora biosurfactant composition 2# was diluted with deionized water to obtain aqueous solutions with concentrations of 0.8% (62.5 times dilution) and 1.5% (33.3 times dilution). The aqueous solution of sophora biosurfactant was treated at room temperature for 30 minutes, and the killing rates of the aqueous solution of sophora biosurfactant against dust mites and human demodex were tested at two concentrations. The results showed that the killing rates of the aqueous solution with a total concentration of 0.8% sophora biosurfactant against dust mites and human demodex were 70% and 78.6%, respectively; the killing rates of the aqueous solution with a total concentration of 1.5% sophora biosurfactant against dust mites and human demodex were 91% and 99.8%.

[0053] From the comparison between Example 1 and Example 2, it can be seen that in an aqueous solution with the same total concentration of sophorolipids, when the mass ratio of acid-type sophorolipids to lactone-type sophorolipids is 1:9, the sophorolipid aqueous solution has a higher killing rate of dust mites and human Demodex.

[0054] Example 3

[0055] In order to explore the interaction between acid-type sophorolipids and lactone-type sophorolipids in killing dust mites and human Demodex, this example draws on the determination method of the joint toxicity index of insecticides.

[0056] The test materials are living dust mites and human demodex mites prepared in advance, and single-dose stock solutions and combined stock solutions of lactone-type sophorolipids and acidic sophorolipids are prepared respectively, and multiple concentration gradients are set to test the median lethal concentration by plate counting method. The test data processing can be processed by data processing software and Excel for data statistical analysis, and the toxicity regression equation, LC 50 , 95% confidence interval, and the toxicity index of each agent and the co-toxicity coefficient (CTC) of the mixture were calculated according to the Sun Yunpei method.

[0057] Measured toxicity index (ATI) = (standard agent LC 50 / Test reagent LC 50 )×100;

[0058] The LC of the standard drug was calculated using the half lethal concentration of lactone-type sophorolipid (B). 50 value.

[0059] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture

[0060] Co-toxicity coefficient (CTC) = (Actual toxicity index (ATI) of mixture / Theoretical toxicity index (TTI) of mixture) × 100

[0061] When the co-toxicity coefficient is greater than 120, it is a synergistic effect; when the co-toxicity coefficient is 80-120, it is an additive effect; when the co-toxicity coefficient is less than 80, it is an antagonistic effect.

[0062] The toxicity of a single agent to dust mites and human Demodex was tested, and the test results are shown in Tables 1 and 2.

[0063] Table 1: Test results of toxicity of sophorolipid compositions to dust mites

[0064]

[0065] Table 2: Results of the toxicity test of the sophorolipid composition against human Demodex mites

[0066]

[0067]

[0068] From the above measurement results, it can be seen that the combination of acid-type sophorolipids and lactone-type sophorolipids in a mass ratio of 10:90-90:10 has a synergistic killing effect on dust mites and human Demodex. Among them, especially after the acid-type sophorolipids and lactone-type sophorolipids are combined in a mass ratio of 10:90 to 40:60, the resulting sophorolipid composition has the most significant killing effect on dust mites and human Demodex. And the compounding of acid-type sophorolipids and lactone-type sophorolipids shows basically the same rules in the co-toxic synergistic effect on dust mites and human Demodex. Among them, the killing function of the sophorolipid composition on dust mites and human Demodex is more significantly affected by the reduction of the proportion of lactone-type sophorolipids. This may be mainly because the acid-type sophorolipid is a biosurfactant that can increase the cell permeability of dust mites and human Demodex, while the lactone-type sophorolipid has a high efficiency in killing dust mites and human Demodex. The combination of the two sophorolipids can have a synergistic effect on the killing of dust mites and human Demodex.

[0069] Example 4

[0070] In this embodiment, the acid-type sophorolipid and the lactone-type sophorolipid are combined in a mass ratio of 10:90-90:10, diluted with deionized water to a total concentration of 0.5%, 5%, 10%, 15%, 20% and other concentrations of sophorolipids, and applied to the more sensitive parts of the human cheek. After 20 minutes, the facial redness is observed. The results show that when the acid-type sophorolipid and the lactone-type sophorolipid are combined in any proportion and the dilution concentration is within the range of 0.5%-20%, there is no irritation to the cheek part, and no redness or itching is caused. It can be seen that the sophorolipid shows good affinity when it is in direct contact with human skin and is compatible with the skin.

[0071] Example 5

[0072] This example is an application example of preparing wet wipes using the sophorolipid composition 2# of Example 2. The sophorolipid composition 2# prepared in Example 2, wherein the mass ratio of acid-type sophorolipid: lactone-type sophorolipid is 40:60, the total mass concentration of sophorolipid is 50%, and the remaining components are deionized water.

[0073] The sophorolipid composition 2# is used to prepare antibacterial and acaricidal wet wipes liquid and wet wipes, and the steps are as follows:

[0074] (1) Prepare 3 parts by mass of glycerin, 6 parts by mass of rose water, 4 parts by mass of a sophorolipid composition, 86.8 parts by mass of deionized water, and 0.2 parts by mass of potassium sorbate as a preservative.

[0075] (2) Add deionized water to the mixing bucket, add glycerin and the sophorolipid composition during the stirring process, and stir evenly. Add rose water to the mixing bucket and stir evenly to obtain an antibacterial and acaricidal sophorolipid wet wipes. The total mass concentration of sophorolipids in the antibacterial and acaricidal sophorolipid wet wipes is 2.0%.

[0076] (3) The prepared antibacterial and acaricidal sophorolipid wet wipes were added to the non-woven fabric at a mass ratio of 2.5:1, and then cut into the required size and packaged to obtain the antibacterial and acaricidal sophorolipid wet wipes. After testing, the killing rate of the wet wipes against human Demodex was 99.5%.

[0077] Example 6

[0078] This example is an application example of preparing shampoo using the sophorolipid composition 1# of Example 1. The sophorolipid composition 1# prepared in Example 1, wherein the mass ratio of acid-type sophorolipid: lactone-type sophorolipid is 10:90, the total mass concentration of sophorolipid is 50%, and the remaining components are deionized water.

[0079] The sophorolipid composition 1# is used to prepare shampoo, and the steps are as follows:

[0080] (1) Prepare phase A and phase B separately:

[0081] Phase A composition: deionized water, 44.14 mass parts; polyquaternium-10, 0.4 mass parts; sophorolipid composition 1#, 2 mass parts; EDTA-2Na, 0.1 mass parts; CDT35 (water, sodium methyl cocoyl taurate, sodium chloride), 4 mass parts; TOP-Rinese CA30H (water, sodium cocoylaminopropionate), 28 mass parts; AMC (sodium cocoylamphoacetate), 10 mass parts; decyl glucoside, 5 mass parts; PEG-120 methyl glucose trioleate, 0.8 mass parts; PEG-150 distearate, 2.1 mass parts; coconut oil methyl monoethanolamide, 1.5 mass parts; PPG-3 octyl ether, 0.3 mass parts; C12-13 alkyl lactate, 0.4 mass parts.

[0082] Phase B composition: flavor, 0.5 mass part; preservative, 0.5 mass part; citric acid, 0.26 mass part.

[0083] (2) Pre-disperse phase A in cold water, heat and stir, raise the temperature to 75-80°C, stir and dissolve evenly, then cool to 45-50°C, add phase B to adjust the consistency according to the viscosity, and after cooling, an antibacterial and mite-killing shampoo can be obtained. Among them, the total mass concentration of sophorolipids is about 1.0%. The shampoo was tested for its killing effect on human Demodex mites, and the killing rate was 98.4%.

[0084] Example 7

[0085] This example is an application example of preparing a mite remover using the sophorolipid composition 1# of Example 1. The sophorolipid composition 1# prepared in Example 1, wherein the mass ratio of acid-type sophorolipid: lactone-type sophorolipid is 10:90, the total mass concentration of sophorolipid is 50%, and the remaining components are deionized water.

[0086] The sophorolipid composition 1# was diluted 33 times with deionized water and ethanol, and 0.5% natural lemon essence was added to prepare a mite remover. The killing effect of the mite remover on bedding dust mites was tested, and the killing rate was 95%.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Use of a composition comprising a sophorolipid composition composed of an acid-type sophorolipid and a lactone-type sophorolipid as the sole active ingredient in killing human Demodex mites, characterized in that: The mass ratio of acid-type sophorolipid to lactone-type sophorolipid is 10:90 to 40:

60.

2. The use according to claim 1, characterized in that: The composition further comprises a solvent, and the solvent is at least one of water, ethanol, glycerol, propylene glycol, butylene glycol, pentanediol, ethyl acetate and ethyl propionate.

3. The use according to claim 1, characterized in that: In the composition, the sophorolipid composition composed of acid-type sophorolipid and lactone-type sophorolipid accounts for 20-90% of the total mass of the composition.

4. The use according to claim 3, characterized in that: In the composition, the sophorolipid composition composed of acid-type sophorolipid and lactone-type sophorolipid accounts for 30-70% of the total mass of the composition.

5. The use according to claim 1 or 4, characterized in that: The composition may also contain a coloring agent or a fragrance.

6. The use according to claim 1, characterized in that: The composition consists only of acid-type sophorolipids and lactone-type sophorolipids, that is, the sophorolipid composition consisting of acid-type sophorolipids and lactone-type sophorolipids accounts for 100% of the total mass of the composition.

7. The use according to any one of claims 1 to 6, characterized in that: The composition is used as an additive to be added into daily cleaning products; or is directly diluted to prepare acaricide for killing human Demodex mites.

8. The use according to claim 7, characterized in that: The daily cleaning and care products include but are not limited to the following products: laundry detergent, laundry powder, washing machine detergent, collar cleaner, down jacket care agent, shampoo, hand soap, hair conditioner, hair mask, facial cleanser, soap, facial cream, lotion, essence, facial mask, toner, shower gel, makeup remover oil, cleansing water, makeup, perfume, wet wipes, and underwear mite remover.

9. The use according to claim 7, characterized in that: In daily cleaning products, the addition amount of the composition is: based on the sophorolipid composition composed of acid-type sophorolipids and lactone-type sophorolipids, the mass percentage of the sophorolipid composition in daily cleaning products is 0.1-20%.

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