A traditional Chinese medicine composition for treating chicken tapeworm disease

Abstract

The present invention belongs to the field of veterinary medicine technology and discloses a traditional Chinese medicine composition for treating tapeworm disease in chickens. The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30-50 parts of Amomum villosum root, 10-30 parts of Clerodendrum scabra root, 10-20 parts of Prunus mume root, 10-20 parts of Punica granatum root, and 5-15 parts of Citrus aurantium immaturus. The raw materials are derived from Chinese medicinal materials or Chinese medicinal extracts equivalent to the amount of the raw materials. The traditional Chinese medicine composition of the present invention is a pure Chinese medicinal preparation. Drug screening tests and clinical treatment trials have verified that the formula of the present invention has a high anthelmintic rate, can significantly improve the cure rate of tapeworm disease, has no palatability, does not affect egg production rate and egg quality, is green and safe, and has good market application value.

Description

Technical Field

[0001] The invention belongs to the field of veterinary medicine, and particularly relates to a traditional Chinese medicine composition for treating chicken tapeworm disease. Background Art

[0002] There are over 40 species of tapeworms that parasitize the chicken intestines. Among them, the four-cornered, spiny, and rounded tapeworms of the genus Ribile are the most harmful to chickens, often presenting clinically as mixed infections. Epidemiological surveys have found that laying hens of all ages can be infected with tapeworms, but are particularly common in chicks and laying hens over 20 days of age. The main clinical manifestations are: large flocks of chickens excreting watery feces or yellowish feces with red or rusty bloody mucus, lethargy, matted feathers, anemia, and pale combs. Infected laying hens experience decreased egg production, characteristic red or rusty stools, and fecal examinations reveal white, sesame-like tapeworm segments.

[0003] Common anthelmintics available on the market include albendazole (for nematodes, lungworms, liver flukes, and tapeworms, with a 4-day withdrawal period for poultry), fenbendazole (for nematodes and tapeworms, affecting egg production, with a 28-day withdrawal period for poultry), levamisole (for nematodes, lungworms, and pig kidneyworms), ivermectin (for nematodes), doramectin (for ticks, fleas, lice, and other external parasites), niclosamide (for tapeworms, with a 28-day withdrawal period for poultry), nitrochlorophenol (for liver flukes in cattle and sheep), iodine ether sulfamethoxazole (for liver flukes in cattle and sheep), triclabendazole (for liver flukes in cattle and sheep), and praziquantel (for tapeworms and schistosomiasis, affecting egg production, with a 28-day withdrawal period for poultry). However, niclosamide is currently the primary treatment for tapeworms in laying hens. However, this drug has significant hepatotoxicity and enterotoxicity, and long-term use carries the risk of drug residues, impacting food safety, and is therefore prohibited for use in laying hens. However, albendazole, fenbendazole, and praziquantel tablets have long withdrawal periods (4-28 days), leaving drug residues in eggs after use. Furthermore, praziquantel and albendazole can affect fertility, egg production, and hatchability (Liu Jingshou et al., Residues of Praziquantel in Eggs from Feed and Its Effect on Hatchability, Heilongjiang Animal Husbandry and Veterinary Medicine, 2009, No. 3). Their use is generally restricted or prohibited during the egg-laying period. Clinically, praziquantel tablets are more effective than albendazole and fenbendazole tablets in removing tapeworms before egg laying. Therefore, to avoid drug residues in eggs and impacts on egg production, these drugs are generally used before egg laying (110 days).

[0004] Regarding the use of traditional Chinese medicine for the prevention and treatment of tapeworms, there are also literature and patent reports using herbs such as betel nut, thunder ball, scutellaria baicalensis, Dichroa chinensis, Artemisia annua, gourd tea, whitehead herb, and smilax china for the prevention and treatment of tapeworms. However, clinical trials have found that while many of these herbs have some effect on improving fecal tapeworm symptoms, their cure and protection rates are low, and they require high dosages to produce palatability, resulting in low clinical use. Some reports, such as using oral solutions formulated with betel nut to expel chicken tapeworms, have shown limited efficacy at low dosages and increased toxicity at high doses. Therefore, in the clinical setting, there is an urgent need for an effective and safe veterinary drug suitable for laying hens that combines high deworming rates with rapid improvement of symptoms such as mental alertness and diarrhea, while maintaining palatability.

[0005] Chinese veterinary medicine has a wide range of sources. In addition to conventional insecticides and antimalarial drugs such as Artemisia annua, Dichroa febrifuga, and Areca catechu, in recent years, screening of ethnic medicines has become a new feasible option. Some of these herbs have the advantages of precise efficacy, green and safe, no toxic side effects, and little negative impact on egg production. In recent years, they have been effectively applied in clinical frontline applications and have considerable market development value. Summary of the Invention

[0006] In view of the problems and shortcomings in the prior art, the present invention aims to provide a Chinese medicine composition for treating chicken tapeworm disease.

[0007] Based on the above purpose, the present invention adopts the following technical solutions:

[0008] A first aspect of the present invention provides a traditional Chinese medicine composition for treating chicken tapeworm disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30-50 parts of Amomum villosum root, 10-30 parts of Clerodendrum scabra root, 10-20 parts of Prunus mume, 10-20 parts of Punica granatum root, and 5-15 parts of Citrus aurantium. The raw materials are derived from traditional Chinese medicines or traditional Chinese medicine extracts equivalent to the raw medicinal amount of the traditional Chinese medicines.

[0009] Preferably, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 35-45 parts of Amomum villosum root, 15-25 parts of Clerodendrum scabra root, 10-15 parts of Prunus mume, 10-15 parts of Punica granatum root, and 5-10 parts of Citrus aurantium immaturum. More preferably, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 40 parts of Amomum villosum root, 20 parts of Clerodendrum scabra root, 15 parts of Prunus mume, 15 parts of Punica granatum root, and 10 parts of Citrus aurantium immaturum.

[0010] The second aspect of the present invention provides a method for preparing a traditional Chinese medicine composition for treating chicken tapeworm disease according to any one of the first aspects above, wherein the method comprises: uniformly mixing the root of Amomum villosum, the root of Clerodendrum scabra, the root of Prunus mume, the root of Punica granatum, and the immature bitter orange in proportion, crushing, and sieving to obtain the traditional Chinese medicine composition.

[0011] More preferably, the particle size of the traditional Chinese medicine composition does not exceed 60 mesh.

[0012] The third aspect of the present invention provides a medicine for treating chicken tapeworm disease, which contains the traditional Chinese medicine composition described in the first aspect.

[0013] Preferably, the medicine further contains pharmaceutically acceptable excipients.

[0014] Preferably, the drug is in oral dosage form.

[0015] Preferably, the drug is in the form of a powder, granules, oral solution, extract or soluble powder. More preferably, the drug is in the form of a powder.

[0016] The fourth aspect of the present invention provides use of the traditional Chinese medicine composition of the first aspect in treating tapeworm disease in chickens, especially in treating tapeworm disease in laying hens.

[0017] Usage and dosage: Mix 250-333kg of feed per 1kg of powder. Feed freely for 3-5 days.

[0018] All components of the present invention can be purchased from the market. The formulation principle of the Chinese medicine composition is as follows:

[0019] Daicao Kombu root: The dried rhizome of Alpinia longipes, a plant of the Zingiberaceae family. It's listed in the "Yunnan Province Traditional Chinese Medicine Standards: Dai Medicine" and is pungent and hot. It enters the spleen and stomach meridians and is primarily used locally to treat rheumatic joint swelling and pain.

[0020] Clerodendrum scabra root: This is the root of the Verbenaceae plant Clerodendrum scabra. It has a pungent, bitter, and warming nature and enters the liver, spleen, and kidney meridians. It promotes qi and strengthens the spleen, dispels wind and dampness, and detoxifies and reduces swelling. It is used to treat stagnation, abdominal distension, hemorrhoids, leukorrhea, rheumatic pain, carbuncles, and swelling.

[0021] Primulina officinalis: The whole herb of Primulina officinalis, a plant of the Primulaceae family, is pungent, bitter, and warm in nature. It dispels wind and dampness, promotes blood circulation, relieves pain, and detoxifies. Its primary component, saponins, possesses strong wind-dispelling and dampness-removing abilities. The paridisin contained in it has a stimulating effect on the isolated uteri of rabbits, guinea pigs, rats, and mice.

[0022] White pomegranate root: This is the root of the Punica granatum or Punica granatum plant of the Punica family. It tastes bitter and astringent, and is slightly warm in nature. It dispels wind and dampness, and kills insects. It is primarily used to treat rheumatic pain.

[0023] Citrus aurantium (Fructus Aurantii Immaturus) is the dried young fruit of Citrus aurantium L. and its cultivated varieties, or Citrus sinensis Osbeck (Citrus sinensis Osbeck), both of the Rutaceae family. It has a bitter, pungent, sour, and slightly cold nature; it enters the spleen and stomach meridians; it dispels qi and resolves stagnation, resolves phlegm, and relieves abdominal distension. It is used to treat internal stagnation, fullness, distension, and pain, heaviness after diarrhea, constipation, phlegm stagnation, qi obstruction, chest pain, chest congestion, and sagging internal organs.

[0024] Clinical symptoms of tapeworm infection in laying hens include loss of appetite, depression, anemia, emaciation, pale combs and mucous membranes, loose stools, and red or rusty-colored, foul-smelling feces. The disease is most common in summer and autumn, from June to November, and is more common in laying hens with compromised immunity. Necropsy findings include hepatosplenomegaly, punctate hemorrhages in the intestinal mucosa, and the presence of numerous live white worms in the duodenum and jejunum. Laying hens exhibit digestive impairment, emaciation, and anemia. Beak-pecking and anal pecking increase. Eggshell color, hardness, and yolk color deteriorate, eggs become smaller, and egg production stagnates or decreases significantly.

[0025] The present invention draws the treatment principle according to the conclusion of Chinese medicine dialectical analysis, and by early clinical screening, it is specially: reuse the pungent and hot medicine Radix Paeoniae Albae strong wind-dispelling and dampness-removing, change the damp-heat internal environment of intestinal tract suitable for tapeworm breeding, and is monarch medicine; be supplemented with the pungent and warm medicine Radix Paeoniae Albae, return liver, spleen, kidney meridian, dispel wind-dispelling and dampness, detoxify and reduce swelling, can control intestinal hemorrhoids and bleeding, insecticide and hemostasis, Prunus mume tastes pungent and bitter, and is warm in nature. Clinically, it is found that except dispelling wind and dampness, it still has a strong anthelmintic effect, and the white pomegranate root tastes bitter and astringent, is slightly warm in nature, insecticide and hemostasis, and the three medicines are ministerial medicines altogether, dispelling wind and dampness, insecticide and hemostasis, and improve the damp-heat internal environment of laying hen intestinal tract; finally, it is assisted by an appropriate amount of Fructus Aurantii Immaturus, breaks up the qi and eliminates stagnation, gives evil with a way out, expelling stagnation and reducing stagnation, and reduces intestinal damage. All medicines are used together, and play the merit of pungent and warm dampness-removing, insecticide and hemostasis altogether.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] (1) The present invention uses TCM dialectical analysis to analyze the etiology and pathogenesis of laying hen tapeworms, and thus derives a treatment principle and a TCM prescription: the root of Amomum villosum is used as the main drug, which strongly dispels wind and dampness, changing the damp and hot internal environment of the intestine that is suitable for tapeworm reproduction; the roots of Croton tiglium, Prunus mume, and Pomegranate scabra are used as the auxiliary drugs, which dispel wind and dampness, kill parasites and stop bleeding, and assist the main drug in expelling parasites and killing parasites; the immature bitter orange is used as the adjuvant drug, which breaks up qi, descends qi, eliminates stagnation, provides a way out for pathogens, expel parasites and eliminates stagnation, and reduces intestinal damage. The combination of these drugs has the effect of pungent, warm, drying dampness, killing parasites and stopping bleeding.

[0028] (2) The Chinese medicine composition of the present invention is a pure Chinese medicine preparation. The results of drug screening tests and clinical treatment tests show that the formula of the present invention has a high anthelmintic rate, can significantly improve the cure rate of tapeworm disease, has no palatability, does not affect egg production rate and egg quality, and is green and safe. In one embodiment, the anthelmintic rate of the Chinese medicine composition prepared by the present invention can reach 96.51%, and the egg production rate data after medication can still be maintained at more than 90%. Therefore, the Chinese medicine composition of the present invention overcomes the shortcomings of the existing technology for the prevention and treatment of tapeworm disease in laying hens (unsatisfactory anthelmintic effect, slow symptom improvement, toxicity of some medicinal materials affecting egg production, etc.), can significantly meet the needs of farms for controlling tapeworms, and has good market application value.

[0029] Other advantages, objects, and features of the present invention will be described in part in the following description and, in part, will be apparent to those skilled in the art upon examination of the following description or may be learned from practice of the present invention. The objects and other advantages of the present invention may be realized and obtained through the following description. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below through examples. It should be understood that the specific examples described herein are only used to explain the present invention and are not intended to limit the present invention.

[0031] It should be noted that, unless there is a conflict, the embodiments and features in the embodiments of this application can be combined with each other. The present application will be described in detail below in conjunction with the embodiments. It should be further noted that the mass of each mass fraction involved in the embodiments is equal.

[0032] Example 1

[0033] The embodiment of the present invention provides a traditional Chinese medicine composition for treating chicken tapeworm disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 40 parts of Amomum villosum root, 20 parts of Clerodendrum scabra root, 15 parts of Prunus mume, 15 parts of Punica granatum root, and 10 parts of Citrus aurantium.

[0034] The raw materials are weighed according to the above-mentioned mass fractions, mixed, crushed, and passed through a 60-mesh sieve. All the sieved materials are mixed to obtain the traditional Chinese medicine composition, and finally the traditional Chinese medicine composition can be packaged.

[0035] Example 2

[0036] The embodiment of the present invention provides a traditional Chinese medicine composition for treating chicken tapeworm disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 30 parts of Amomum villosum root, 25 parts of Clerodendrum scabra root, 20 parts of Prunus mume, 10 parts of Punica granatum root, and 15 parts of Citrus aurantium.

[0037] Weigh each raw material according to the above-mentioned mass fraction, mix, crush, and pass through a 60-mesh sieve, then mix all the sieve-downs to obtain the traditional Chinese medicine composition, and finally package the traditional Chinese medicine composition.

[0038] Example 3

[0039] The embodiment of the present invention provides a traditional Chinese medicine composition for treating chicken tapeworm disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 50 parts of Amomum villosum root, 15 parts of Clerodendrum scabra root, 10 parts of Prunus mume, 20 parts of Punica granatum root, and 5 parts of Citrus aurantium.

[0040] Weigh each raw material medicine according to the above parts by mass, mix, pulverize, and pass through a 60-mesh sieve. After mixing all the materials passing through the sieve, the said traditional Chinese medicine composition is obtained. Finally, the traditional Chinese medicine composition is packaged.

[0041] Experimental Example 1: Acute Toxicity Test

[0042] 1. Test Materials

[0043] (1) Test animals: 50 Kunming mice, each weighing about 15 g, with half males and half females. They were purchased from Henan Experimental Animal Center, certificate number: SCXK(Yu)2020 - 0012. After purchase, the mice were adaptively raised for 3 - 5 days in the environment, and then used for subsequent tests when their body weights were about 18 - 22 g per mouse.

[0044] (2) Test drugs: A traditional Chinese medicine composition for treating chicken tapeworm disease was prepared according to Example 1. It was specifically prepared by the Traditional Chinese Medicine Laboratory of the National Enterprise Technology Center of Henan MuXiang Animal Pharmaceutical Co., Ltd. in June 2021. Hereinafter, it is referred to as "Three-Root Tapeworm Powder", specification: 1 kg / bag.

[0045] 2. Test Methods

[0046] (1) Preliminary test: Take 20 test mice, randomly divide them into 5 groups, with 4 mice in each group, half males and half females. After the mice were fasted overnight without water deprivation for 10 h, they were used for the test. According to the literature data and expert experience, after weighing the body weight of each mouse, the drug was mixed with 0.5 mL of normal saline according to the dose of 5 g drug / kgBW, and the mice were gavaged. After gavage, the mice were allowed to eat freely and observed for 24 h. If no death occurred, a formal test was arranged.

[0047] (2) Formal test: Take 30 test mice, weigh their body weights, and randomly divide them into 3 groups, with 10 mice in each group, half males and half females, namely the blank group, drug group 1, and drug group 2 (the drug groups are 2 parallel replicates). Before each feeding, the mice were fasted overnight without water deprivation for 10 h and then used for the test. Among them, each mouse in drug group 1 and drug group 2 was dosed once a day according to the dose of 5 g drug / kgBW. When dosing, the drug was mixed with 0.5 mL of normal saline to make a suspension for gavage of the mice; the blank group was given 0.5 mL of normal saline for gavage. After gavage, the mice were allowed to eat freely. The death situation and mental state of the mice were observed continuously for 7 d, and the body weights of the mice in each group were weighed and recorded. Some results are shown in Table 1 and Table 2.

[0048] 3. Test Results and Analysis

[0049] (1) Physical condition: Both the preliminary and formal experiments showed that after administration, the mice in the drug group ate normally, gained weight, moved freely, had smooth fur, and had normal urination and defecation, no abnormal secretions, and no adverse reactions. As shown in Table 1, no mice in the drug group died after 7 days of observation. Gross autopsy and pathological examinations showed no abnormalities in the major organs of the mice in the drug group, and no differences were observed with the blank control group by naked eye.

[0050] Table 1 Death of mice in each group with acute toxicity

[0051]

[0052] (2) Body weight: As shown in Table 2, although the weight gain rate of mice in drug group 1 and drug group 2 was slightly higher than that in the blank control group, there was no significant difference after statistical analysis.

[0053] Table 2 Death of mice in each group with acute toxicity

[0054]

[0055]

[0056] In summary, according to the Veterinary Drug Acute Toxicity Test (LD 50 If no mice die during the test period and the mice are in good spirits after administration, and no deaths occur in the group receiving the drug at a dose of 5000 mg / kg, it is not necessary to determine the LD50. 50 The dosage of the three-root tapeworm powder prepared by the present invention has reached 5000 mg / kg, which indicates that the clinical application dosage of the traditional Chinese medicine composition prepared by the present invention is relatively safe and has no safety risks.

[0057] Test Example 2: Tapeworm removal test in laying hens

[0058] 1. Test materials

[0059] (1) Experimental animals: 9,300 270-day-old sea blue brown laying hens were obtained from Hengmao Farm in Dongyang Village, Weishi County, Kaifeng City. In the morning before feeding, fresh feces were examined to identify chickens infected with tapeworms. After microscopic examination by the Poultry Disease Research Institute of Henan Agricultural University, it was found that the main infections were Rileya spinosum and Rileya rotundifolia. 41 infected chickens were selected and placed in an isolation feeding device for single feeding. They were observed for 24 hours and their feces were collected. The infection was confirmed by microscopic examination again to confirm whether they were infected with tapeworms. Finally, 36 infected chickens that met the experimental requirements were selected and used as experimental chickens.

[0060] (2) Experimental drugs: Three-root tapeworm powder, specification: 1kg / bag, prepared by the Traditional Chinese Medicine Laboratory of Henan Muxiang Animal Pharmaceutical Co., Ltd. in July 2021 according to Example 1 of the present invention; veterinary praziquantel tablets, 0.2g / tablet, produced by Sichuan Hengtong Animal Health Biotechnology Co., Ltd., production batch number: 201208; Anthelmintic powder (veterinary trade name: Animal and Poultry Insect Killer; ingredients: lice, thunder balls, black plums, Quisqualis, Corydalis cyrtonema, Terminalia chebula, Areca nut, dried ginger, rhubarb, costus root, and Torreya grandis), 25g / bag, Sichuan Forever Animal Husbandry Pharmaceutical Co., Ltd., production batch number: 210307.

[0061] 2. Test methods

[0062] (1) Experimental grouping: 36 experimental chickens were randomly divided into 6 groups according to their body weight (the average body weight of the experimental chickens in each group was not significantly different), namely, Chinese medicine group I (low-dose group of Sangentaeshockum powder), Chinese medicine group II (medium-dose group of Sangentaeshockum powder), Chinese medicine group III (high-dose group of Sangentaeshockum powder), Western medicine control group (praziquantel tablets group), Chinese medicine control group (anthelmintic powder group), and blank control group (free drinking water group).

[0063] (2) Administration: The concentrations of Sangentaesin powder added to the feed of the Chinese medicine group I, Chinese medicine group II, and Chinese medicine group III were 0.15%, 0.3%, and 0.6%, respectively, and the drug was administered continuously for 5 days. The dosage and method of praziquantel tablets and anthelmintic powder added to the feed of the Western medicine control group and the Chinese medicine control group were in accordance with the pharmacopoeial instructions for continuous administration for 5 days. The blank control group was not supplemented with any drugs and served as the blank control group. All experimental chickens had free access to food and water.

[0064] 3. Test results and analysis

[0065] (1) Observation indicators

[0066] Anthelmintic effect: The feces of 6 chickens in each group were collected every day after the start of medication, and the parasites were found and counted, which was recorded as the number of parasites expelled on that day; then after counting the number of eggs laid on the 5th day of medication, all the test chickens were killed, and the intestines were examined using the full collection method to find the number of residual parasites. Finally, the anthelmintic results were calculated, anthelmintic rate (%) = (sum of the number of parasites expelled on the 1st and 2nd days) / (sum of the number of parasites expelled on the 1st to 5th days + the number of residual parasites) × 100%, and the results are shown in Table 3.

[0067] Egg laying situation: the number of eggs laid by each group was counted, and the total number of eggs laid by each group 5 days after medication was counted to observe the effect of medication on the production performance of laying hens (number of eggs laid). The average egg laying rate (%) = total number of eggs laid by the group test chickens 5 days after medication / (5 days × 6 chickens) × 100%. The results are shown in Table 4.

[0068] (2) Results and analysis

[0069] Deworming results and analysis:

[0070] Table 3 Statistics of anthelmintic effect after medication in each group

[0071]

[0072] After medication, tapeworms were gradually expelled and essentially ceased to be expelled after 48 hours. The total number of dewormings in each group within 48 hours was then counted. As can be seen in Table 3, egg-laden segments were found in the feces of the test chickens in the blank control group within 48 hours, totaling one. Praziquantel had the best deworming effect, followed by the medium- and high-dose groups of the three-root tapeworm powder prepared in Example 1 of the present invention, with deworming rates exceeding 90%, meeting clinical deworming needs. The high-dose group had a deworming rate of 96.51%, very close to the deworming effect of the Western medicine control group. In this experiment, the deworming powder was not particularly effective, with the deworming rate data not even exceeding 80%. This is consistent with the inventor's clinical experience as a frontline clinical technician that veterinary deworming powders produced by multiple companies were ineffective and required double the dose. Furthermore, double the dose had a palatability issue, likely related to the strong odors of betel nut and costus root. However, the three-root tapeworm powder prepared by the present invention was not found to be palatable in the low, medium and high dose groups, and the mental state of the chickens basically returned to normal from depression 3 days after medication, which is basically consistent with the above results.

[0073] Egg production and analysis:

[0074] Table 4 Statistics of egg production in each group after medication

[0075]

[0076]

[0077] As shown in Table 4, praziquantel tablets have a side effect on egg production, reducing egg production to less than 70%, consistent with literature reports that praziquantel has reproductive toxicity, affecting the fertility and egg production rates of laying hens. Furthermore, the presence of a withdrawal period makes it unsuitable for use during the laying period. However, the traditional Chinese medicine anthelmintic powder and the present invention's three-root tapeworm powder have minimal impact on egg production and can be used safely during the laying period.

[0078] In summary, based on the results of the comprehensive anthelmintic effect and laying hen egg production, the Chinese medicine composition prepared in this application is slightly inferior to the Western medicine control group of quinolone in anthelmintic effect, but it is friendly to the laying performance of laying hens, and the prescription does not contain blood-activating or nephrotoxic ingredients, and there is no risk of drug residue. Compared with the Chinese medicine control group of anthelmintic powder, the anthelmintic effect of this application is significantly higher than that of the Chinese medicine anthelmintic powder. Therefore, the Chinese medicine composition prepared in this application has better overall performance.

[0079] Test Example 3: Tapeworm removal test in laying hens

[0080] 1. Experimental process

[0081] In August 2021, at the Taiping Layer Farm in Mudan District, Heze City, Shandong Province, there were 8,200 350-day-old Hy-Line Gray laying hens. Farmers reported that the chickens had recently experienced varying degrees of reduced feed intake, lethargy, drooping wings, messy fur, diarrhea (rusty loose stools), weight loss, and white worm segments were visible in some feces. An autopsy revealed that the small intestine was blocked by worms and there were obvious punctate bleeding, and worms could be found on the intestinal wall. The Watanabe egg concentration method was used to collect feces, and tapeworm segments and tapeworm eggs were found. The on-site practicing veterinarian determined that the chickens were infected with typical tapeworm disease (mainly Rileyi spinosum and Rileyi spp.).

[0082] After communicating with the on-site veterinarian, the three-root tapeworm powder prepared by the present invention was used for treatment, and the chickens with typical symptoms were marked, and 1 kg of the medicine was mixed with 250 kg of feed (the amount was increased as appropriate if used alone) and used for 5 consecutive days.

[0083] 2. Test results and analysis

[0084] During a follow-up visit after the medication, the chickens' spirits significantly improved, their feed intake gradually recovered to pre-disease levels, and the rust-colored diarrhea had essentially disappeared. Intestinal examinations of eight chickens marked before the medication were performed, revealing no bleeding. One chicken had a single residual worm, while the remaining seven had no residual worms. Therefore, the results demonstrate that the Sangen Taenia powder prepared by the present invention effectively prevents and treats tapeworms in laying hens.

[0085] Therefore, considering the above test results and the cost of medication, the recommended dosage of 1 kg of the Chinese medicine formula of the present invention mixed with 300 kg of feed for 3-5 days can significantly improve the cure rate of tapeworm disease, and it has a definite therapeutic effect, high cost-effectiveness, and is green and safe.

[0086] In summary, the present invention effectively overcomes the deficiencies in the prior art and has a high industrial application value. The above embodiments serve to illustrate the substantial content of the present invention, but are not intended to limit the scope of protection of the present invention. Those skilled in the art will appreciate that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of protection of the technical solutions of the present invention.

Claims

1. A Chinese medicinal composition for treating chicken tapeworm disease, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30-50 parts of Amomum villosum root, 10-30 parts of Clerodendrum scabra root, 10-20 parts of Prunus mume, 10-20 parts of Punica granatum root, and 5-15 parts of Citrus aurantium immaturus. The raw materials are derived from traditional Chinese medicines or traditional Chinese medicine extracts equivalent to the raw medicinal amount of the traditional Chinese medicines.

2. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 35-45 parts of Amomum villosum root, 15-25 parts of Clerodendrum scabra root, 10-15 parts of Prunus mume, 10-15 parts of Punica granatum root, and 5-10 parts of Citrus aurantium immaturum.

3. The Chinese medicine composition according to claim 2, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 40 parts of Amomum villosum root, 20 parts of Clerodendrum scabra root, 15 parts of Prunus mume, 15 parts of Punica granatum root, and 10 parts of Citrus aurantium immaturum.

4. The method for preparing the Chinese medicinal composition for treating chicken tapeworm disease according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: uniformly mixing the root of Amomum villosum, the root of Clerodendrum scabra, the root of Prunus mume, the root of Punica granatum and the immature bitter orange according to a certain proportion, crushing the mixture and sieving the mixture to obtain the traditional Chinese medicine composition.

5. A drug for treating chicken tapeworm disease, characterized in that: The medicine contains the traditional Chinese medicine composition according to any one of claims 1 to 3.

6. The drug according to claim 5, characterized in that The medicine also contains pharmaceutically acceptable excipients.

7. The drug according to claim 6, characterized in that The drug is in oral dosage form.

8. The drug according to claim 7, characterized in that The medicine is in the form of powder, granules, oral solution or extract.

9. The drug according to claim 8, characterized in that The medicine is in the form of powder.

10. Use of the traditional Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicament for treating chicken tapeworm disease.

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