Method and apparatus for ranking material's ability to adhere cells

Abstract

The application relates to the field of material grading, and provides a material cell adhesion ability grading method and device. The method comprises the following steps: arranging a sample base in a connecting part of a tube body part and a pointed end part of a centrifugal tube, fixing a material to be tested in a groove of the sample base, and obtaining a first centrifugal tube to be processed; pouring a blood mixture into the first centrifugal tube to be processed, obtaining a second centrifugal tube to be processed; performing a centrifugal operation on the second centrifugal tube to be processed, obtaining a material to be observed according to the material to be tested after the centrifugal operation; and determining a cell adhesion ability grade of the material to be tested according to the number of residual cells on the material to be observed. The material cell adhesion ability grading method provided by the application can simulate the interaction between the material and the cells in an actual cell separation scene, and grade the cell adhesion ability of the material to be tested, so that the strength of the cell adhesion ability of the material can be accurately judged, and the accuracy of the cell adhesion ability grading of the material is improved.

Description

Technical Field

[0001] This application relates to the field of material grading technology, specifically to a method and apparatus for grading the ability of materials to adhere to cells. Background Technology

[0002] In treating hematological malignancies, blood samples are collected from the patient and then separated into immune cells using density gradient centrifugation. These immune cells are then genetically modified and amplified to obtain immune cells that can be reinfused into the patient to kill cancer cells. During this process, when using fully automated density gradient separation equipment to separate the blood sample or different components of the amplified immune cells, some cells adhere to the centrifuge vessel wall. This reduces the number of immune cells actually obtained to kill cancer cells, thus affecting the patient's treatment outcome.

[0003] To obtain centrifuge cup wall materials that do not easily adhere to cells, it is necessary to determine the cell adhesion ability of various materials. The traditional method is to estimate the cell adhesion ability of a material based on its surface energy, then make a centrifuge cup using this material, and test the cell adhesion ability of the centrifuge cup made of this material in a real-world environment to ultimately determine the material's cell adhesion ability.

[0004] However, surface energy is a constant property of a material. When the same material adheres to different cells of the same type or different types of cells, its surface energy does not change. Therefore, it is impossible to distinguish the adhesion ability of the material to different cells of the same type or different types of cells. In fact, since the degree of adhesion between each cell and the material often involves the combined effect of multiple proteins of the cell and different binding sites of the material, the adhesion between different cells of the same type and different types of cells and the same material may be different. Using the surface energy of the material to measure the adhesion ability to cells has low accuracy. Summary of the Invention

[0005] This application provides a method and apparatus for grading the cell adhesion ability of materials, in order to solve the technical problem that the accuracy of using the surface energy of materials to calibrate their cell adhesion ability is low.

[0006] In a first aspect, embodiments of this application provide a method for grading the cell adhesion ability of materials, including:

[0007] A sample base is placed in the connection between the body and tip of a centrifuge tube, and the material to be tested is fixed in the groove of the sample base to obtain a first centrifuge tube to be processed; the bottom opening of the body is connected to the top opening of the tip, the material to be tested is in sheet form, and the groove opening direction is consistent with the top opening direction of the centrifuge tube.

[0008] The blood mixture was poured into the first centrifuge tube to be processed, resulting in a second centrifuge tube to be processed.

[0009] The second centrifuge tube to be processed is centrifuged, and the material to be observed is obtained based on the material to be tested after centrifugation.

[0010] The cell adhesion ability level of the test material is determined based on the number of residual cells on the test material.

[0011] In one embodiment, fixing the material to be tested in the groove of the sample base includes:

[0012] After the material to be tested is placed in the groove of the sample base, a pressure plate with a through hole is fixed to the groove opening side to fix the material to be tested in the groove; wherein, the through hole is connected to the groove opening.

[0013] In one embodiment, obtaining the material to be observed from the material to be tested after centrifugation includes:

[0014] The test material was rinsed with physiological saline after centrifugation to obtain the rinsed material;

[0015] The cell contact surface of the rinsed material is bonded to a glass slide to obtain the material to be observed; the cell contact surface is the side of the rinsed material that is in direct contact with the blood mixture.

[0016] In one embodiment, before determining the cell adhesion ability level of the test material based on the number of residual cells on the test material, the following steps are included:

[0017] The material under observation is observed using a microscope, and multiple residual numbers are obtained based on the number of residual cells on the cell contact surface under multiple different fields of view.

[0018] In one embodiment, determining the cell adhesion ability level of the test material based on the number of residual cells on the test material includes:

[0019] Based on the number of residues and the morphology of the residues corresponding to the number of residues, determine the multiple cell area values ​​corresponding to the multiple numbers of residues;

[0020] Calculate the average of the multiple cell area values, and divide the average by the area of ​​the cell contact surface to obtain the percentage of residual cell coverage;

[0021] The cell adhesion ability level of the test material is determined based on the percentage of residual cell coverage.

[0022] In one embodiment, determining the cell adhesion ability level of the test material based on the residual cell coverage percentage includes:

[0023] If the residual cell coverage is greater than 70% and less than or equal to 90%, then the cell adhesion ability of the test material is determined to be Level 1.

[0024] If the residual cell coverage is greater than 50% and less than or equal to 70%, then the cell adhesion ability of the test material is determined to be level two.

[0025] If the residual cell coverage is greater than 30% and less than or equal to 50%, then the cell adhesion ability of the test material is determined to be level three.

[0026] If the residual cell coverage ratio is greater than 10% and less than or equal to 30%, then the cell adhesion ability of the test material is determined to be level four.

[0027] If the residual cell coverage percentage is greater than 0% and less than or equal to 10%, then the cell adhesion ability of the test material is determined to be level five.

[0028] In one embodiment, obtaining the material to be observed from the material to be tested after centrifugation includes:

[0029] The cell contact surface of the test material after centrifugation is bonded to a glass slide to obtain the test material; the cell contact surface is the side of the test material after centrifugation that is in direct contact with the blood mixture.

[0030] In one embodiment, determining the cell adhesion ability level of the test material based on the number of residual cells on the test material includes:

[0031] If the number of residual cells on the material to be observed is zero, then the cell adhesion ability of the material to be tested is determined to be level six.

[0032] In one embodiment, the step of placing the sample base within the connection between the centrifuge tube body and the tip includes:

[0033] Multiple steel balls are placed inside the tip.

[0034] In a second aspect, embodiments of this application provide a material cell adhesion ability grading device for implementing the material cell adhesion ability grading method described in the first aspect, comprising: centrifuge tube, sample base, tablet and steel ball;

[0035] The centrifuge tube includes a tube body and a tip, wherein the size of the top opening of the tip is larger than the size of the bottom opening of the tip, and the bottom opening of the tube body is connected to the top opening of the tip.

[0036] The sample base is disposed in the connection between the tube body and the tip, and the sample base is provided with a groove, the groove opening direction being consistent with the top opening direction of the centrifuge tube.

[0037] The pressure plate is disposed on the groove opening side of the groove, and the pressure plate is provided with a through hole, which is connected to the groove opening;

[0038] The steel ball is disposed inside the tip.

[0039] The method for grading the cell adhesion ability of materials provided in this application involves placing a sample base within the connection between the body and tip of a centrifuge tube, fixing the test material in the groove of the sample base to obtain a first centrifuge tube, then pouring a blood mixture into the first centrifuge tube to obtain a second centrifuge tube, and then centrifuging the second centrifuge tube. The test material after centrifugation is used to obtain the observation material, and finally, the cell adhesion ability level of the test material is determined based on the number of residual cells on the observation material. This method directly contacts the test material with the blood mixture, simulating a real cell separation scenario under centrifugation. In this scenario, the cell adhesion ability of the test material is graded based on the number of residual cells on the observation material. Because it simulates the interaction between material and cells in a real cell separation scenario and grades the cell adhesion ability of the test material, it can accurately determine the strength of the material's cell adhesion ability, thus improving the accuracy of grading the material's cell adhesion ability. Attached Figure Description

[0040] To more clearly illustrate the technical solutions in this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0041] Figure 1 This is one of the flowcharts illustrating the material cell adhesion ability grading method provided in the embodiments of this application;

[0042] Figure 2 This is a second schematic flowchart of the material cell adhesion ability grading method provided in the embodiments of this application;

[0043] Figure 3 This is the third flowchart illustrating the method for grading the ability of materials to adhere to cells provided in the embodiments of this application;

[0044] Figure 4 This is a schematic diagram of the material cell adhesion ability grading device provided in the embodiments of this application.

[0045] Figure label:

[0046] 1-Centrifuge tube; 11-Tube body; 12-Tip; 2-Sample base; 21-Groove; 3-Pressure plate; 31-Through hole; 4-Steel ball. Detailed Implementation

[0047] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0048] Figure 1 This is one of the flowcharts illustrating the method for grading the ability of materials to adhere to cells provided in the embodiments of this application. (Refer to...) Figure 1 This application provides a method for grading the cell adhesion ability of materials, which may include:

[0049] 101. Place the sample base in the connection between the tube body and the tip of the centrifuge tube, and fix the material to be tested in the groove of the sample base to obtain the first centrifuge tube to be processed.

[0050] The bottom opening of the tube body is connected to the top opening of the tip. The material to be tested is sheet-like, and the groove opening direction is consistent with the top opening direction of the centrifuge tube.

[0051] 102. Pour the blood mixture into the first centrifuge tube to be processed, thus obtaining the second centrifuge tube to be processed;

[0052] 103. Centrifuge the second centrifuge tube to be processed, and obtain the material to be observed based on the material to be tested after centrifugation.

[0053] 104. Determine the cell adhesion ability level of the material to be tested based on the number of residual cells on the material to be observed.

[0054] In step 101, the sample base is placed in the connection between the body and tip of the centrifuge tube. The shape and size of the sample base and the connection are not limited here, as long as the sample base and the connection are compatible. The material of the sample base and the centrifuge tube are also not limited here, as long as they meet the implementation requirements.

[0055] In this embodiment, the sample base is circular in shape, with a diameter of 20 mm and a thickness of 6 mm, and is made of stainless steel. The connecting part is also circular in shape. The centrifuge tube has a capacity of 50 ml and an inner diameter of 28 mm. Since the connecting part is the connection between the bottom opening of the tube body and the top opening of the tip, it can be assumed that the inner diameter of the connecting part is 28 mm, which is sufficient for the sample base to be set.

[0056] The shape and size of the groove in the sample base, as well as the shape and material of the material to be tested, are not limited here, as long as the groove can accommodate the material to be tested.

[0057] In this embodiment, the groove is square with a side length of 14 mm and a depth of 3 mm, and the material to be tested is also square with a side length of 12 mm.

[0058] It should be noted that holes (non-through holes) can be drilled at the edge of any side of the test material before fixing the test material in the groove of the sample base. Since the side of the test material near the groove opening is the side that is in direct contact with the blood mixture, it is possible to first determine whether there is a hole on the side of the test material near the groove opening. This allows you to determine which side of the test material is in direct contact with the blood mixture after removing the test material. For example, after fixing the test material in the groove of the sample base, if it is determined that there is a hole on the side of the test material near the groove opening, then after removing the test material, it can be determined that the side of the test material with the hole is the side that is in direct contact with the blood mixture.

[0059] In step 102, the blood mixture can be poured into the first centrifuge tube until the height of the blood mixture reaches the 20 ml mark on the first centrifuge tube.

[0060] It should be noted that, during the repetition of this embodiment, it is necessary to ensure that the height of each poured blood mixture reaches the 20 ml mark of the first centrifuge tube to be processed, so as to ensure that the number of cells in the blood mixture is consistent in each implementation and reduce the error between different implementation processes.

[0061] In step 103, the second centrifuge tube to be processed can be placed into a regular centrifuge for centrifugation. The centrifugal force can range from 200 gravitational accelerations to 700 gravitational accelerations, and the centrifugation time can be 20 minutes.

[0062] The method for grading the cell adhesion ability of materials provided in this embodiment involves placing a sample base within the connection between the body and tip of a centrifuge tube, and fixing the test material in the groove of the sample base to obtain a first centrifuge tube. A blood mixture is then poured into the first centrifuge tube to obtain a second centrifuge tube. The second centrifuge tube is then centrifuged. The test material after centrifugation is used to obtain the observation material. Finally, the cell adhesion ability level of the test material is determined based on the number of residual cells on the observation material. This method directly contacts the test material with the blood mixture, simulating a real cell separation scenario under centrifugation. In this scenario, the cell adhesion ability of the test material is graded based on the number of residual cells on the observation material. Because it simulates the interaction between materials and cells in a real cell separation scenario and grades the cell adhesion ability of the test material, it can accurately determine the strength of the material's cell adhesion ability, thus improving the accuracy of grading the cell adhesion ability of materials.

[0063] Furthermore, traditional methods require fabricating materials into centrifuge cups for actual testing to ultimately determine the material's cell adhesion ability. This necessitates significant resources for centrifuge cup fabrication. If a material is found to have strong adhesion, centrifuge cups made from that material become unusable, resulting in a large waste of centrifuge cups. The method in this embodiment can quickly screen a large number of materials without requiring fabricated centrifuge cups for testing to determine the material's cell adhesion ability, thus reducing material consumption and centrifuge cup waste.

[0064] In one embodiment, fixing the material to be tested in a groove in the sample base may include:

[0065] After the material to be tested is placed in the groove of the sample base, a pressure plate with a through hole is fixed to the groove opening side to fix the material to be tested in the groove.

[0066] The through hole is connected to the slot.

[0067] The shape, size, and material of the tablet are not limited here, as long as they can completely cover the groove opening.

[0068] In this embodiment, the pressure plate is circular in shape, with a diameter of 20 mm, and is made of stainless steel, and can completely cover the groove opening side.

[0069] The shape and size of the through hole are not limited here. In this embodiment, the through hole is circular and has a diameter of 10 mm.

[0070] Since the density of the test material may be lower than that of blood, after the blood mixture is poured into the first centrifuge tube, the test material may float out of the groove. During subsequent centrifugation, both sides of the test material will be in full contact with the cells, which will disrupt the simulation of the actual centrifugation environment (in the actual centrifugation environment, the blood mixture will only contact the inside of the centrifuge cup, that is, only one side of the material). On the other hand, the fact that both sides of the test material will be in full contact with the cells will also increase the tediousness of counting the residual cells on the surface of the material.

[0071] In this embodiment, by fixing a pressure plate with a through hole to the groove opening side, the material to be tested is fixed in the groove, and the through hole is connected to the groove opening. On the one hand, this can prevent the material to be tested from floating up and damaging the simulation environment and increasing the tediousness of cell counting. On the other hand, the blood mixture can flow into the groove through the through hole and the groove opening without affecting the contact between the material to be tested and the blood mixture during centrifugation.

[0072] Figure 2 This is the second schematic flowchart of the material cell adhesion ability grading method provided in the embodiments of this application. (Refer to...) Figure 2 In one embodiment, obtaining the material to be observed based on the material to be tested after centrifugation may include:

[0073] 201. Rinse the test material after centrifugation with physiological saline to obtain the rinsed material;

[0074] 202. Bond the cell-contact surfaces of the rinsed material to a glass slide to obtain the material to be observed;

[0075] The cell contact surface is the side of the material that comes into direct contact with the blood mixture after rinsing.

[0076] In step 201, a handheld pipette can be used to draw 1 ml of physiological saline to rinse the surface of the test material after centrifugation. This process is repeated 3 times to remove some cells that are weakly adhered to the test material, thus avoiding their influence on increasing the grading error.

[0077] In this embodiment, the test material is rinsed with physiological saline after centrifugation, and the cell contact surface of the rinsed material is bonded to a glass slide, so that the cell state on the test material can more accurately characterize the adhesion ability of the test material to cells.

[0078] In one embodiment, before determining the level of cell adhesion ability of the test material based on the number of residual cells on the test material, the following may be included:

[0079] The material under observation is observed using a microscope, and multiple residual cell counts are obtained based on the number of residual cells on the cell contact surface under multiple different fields of view.

[0080] This embodiment uses multi-field observation to obtain the number of residual cells under multiple different fields of view. Therefore, the number of residual cells under multiple different fields of view can be used to verify each other and reduce the error in the number of residual cells.

[0081] Figure 3 This is the third schematic flowchart of the material cell adhesion ability grading method provided in the embodiments of this application. (Refer to...) Figure 3 In one embodiment, determining the cell adhesion ability level of the test material based on the number of residual cells on the test material may include:

[0082] 301. Based on the number of residues and the morphology of the residue cells corresponding to the number of residues, determine the area values ​​of multiple cells corresponding to multiple residue numbers;

[0083] 302. Calculate the average of multiple cell area values, and divide the average by the area of ​​the cell contact surface to obtain the percentage of residual cell coverage.

[0084] 303. If the residual cell coverage is greater than 70% and less than or equal to 90%, the cell adhesion ability of the test material is determined to be Level 1.

[0085] 304. If the residual cell coverage is greater than 50% and less than or equal to 70%, the cell adhesion ability of the test material is determined to be level two.

[0086] 305. If the residual cell coverage is greater than 30% and less than or equal to 50%, the cell adhesion ability of the test material is determined to be level three.

[0087] 306. If the residual cell coverage is greater than 10% and less than or equal to 30%, the cell adhesion ability of the test material is determined to be level four.

[0088] 307. If the residual cell coverage is greater than 0% and less than or equal to 10%, the cell adhesion ability of the test material is determined to be level five.

[0089] In step 301, for example, if the number of residual cells obtained in the first field of view is A, and the shape of these A cells in the first field of view is rice grain-shaped or round, then the area of ​​all A cells on the cell contact surface is calculated based on the number of residual cells A and the shape of these A cells to obtain the first cell area value. Similarly, the area of ​​all cells on the cell contact surface in other fields of view is calculated to obtain multiple cell area values.

[0090] In step 302, the average value of the cell area under multiple fields of view is calculated, and the average value is divided by the area of ​​the cell contact surface to obtain the percentage of residual cell coverage.

[0091] It should be noted that in practical applications, there is no strict timing relationship between steps 303 and 307; that is, they can be executed simultaneously, or any one step can be executed first, depending on the actual needs, and no restrictions are imposed here.

[0092] In addition, the interval division of the residual cell coverage ratio can be adjusted according to actual needs to make the grading more accurate.

[0093] This embodiment determines the residual cell coverage ratio by comparing the average cell area value from multiple fields of view with the area of ​​the cell contact surface. This ratio is then used to classify the cell adhesion ability of the test material, thus providing accurate classification.

[0094] In one embodiment, obtaining the material to be observed from the material to be tested after centrifugation may include:

[0095] The cell contact surface of the material to be tested after centrifugation is bonded to a glass slide to obtain the material to be observed;

[0096] The cell contact surface is the side of the test material that comes into direct contact with the blood mixture after centrifugation.

[0097] In this embodiment, the test material is directly bonded to the glass slide without rinsing after centrifugation, which allows for the determination of the test material's adhesion to cells without rinsing during subsequent observations.

[0098] In one embodiment, determining the cell adhesion ability level of the test material based on the number of residual cells on the test material includes:

[0099] If the number of residual cells on the material to be observed is zero, then the ability of the material to adhere to cells is determined to be level six.

[0100] The material to be observed is unwashed. Even without washing, the material can be observed using a microscope. Based on the number of residual cells on the cell contact surface under multiple different fields of view, multiple residual counts are obtained. If multiple residual counts are all zero, it indicates that the material to be tested has a very weak ability to adhere to cells.

[0101] In this embodiment, when the number of residual cells on the material to be observed is zero, the cell adhesion ability of the material to be tested is determined to be level six, so as to characterize the weak cell adhesion ability of the material to be tested.

[0102] In one embodiment, placing the sample base within the connection between the centrifuge tube body and the tip may include:

[0103] Multiple steel balls are placed inside the tip.

[0104] The size, quantity, and material of the steel ball are not specified here.

[0105] In this embodiment, the steel ball has a diameter of 6 mm and is made of stainless steel.

[0106] Because the blood mixture flows into the tip of the centrifuge tube from the gap between the sample base and the centrifuge tube, and then flows out from the bottom opening of the tip, it causes waste of blood mixture and cells, affecting cell count and thus affecting the grading of material adhesion ability.

[0107] This embodiment slows down the flow rate of the blood mixture by placing multiple steel balls inside the tip, thereby reducing the consumption of the blood mixture and cells, improving the accuracy of cell counting, and thus improving the accuracy of grading the material's adhesion ability.

[0108] In one embodiment, a through hole can be made at the bottom of the groove of the sample base to facilitate the removal of the sample base for reuse using tweezers or a hook.

[0109] The shape and size of the through hole are not limited here. In this embodiment, the through hole is a rectangular through hole with a length of 7 mm and a width of 2 mm.

[0110] Alternatively, the pellets and test material after centrifugation can be removed using tweezers or similar tools.

[0111] This embodiment allows for easy removal of the sample base using tweezers or a hook after the centrifugation process by opening a passage at the bottom of the groove, so that it can be reused later.

[0112] The following describes the material adhesion cell ability grading device provided in the embodiments of this application. The material adhesion cell ability grading device described below and the material adhesion cell ability grading method described above can be referred to in correspondence.

[0113] Figure 4 This is a schematic diagram of the material cell adhesion ability grading device provided in an embodiment of this application. (Refer to...) Figure 4 This application provides a material cell adhesion ability grading device for implementing the aforementioned material cell adhesion ability grading method, which may include: centrifuge tube 1, sample base 2, pressure plate 3 and steel ball 4;

[0114] The centrifuge tube 1 includes a tube body 11 and a tip 12. The top opening of the tip 12 is larger than the bottom opening of the tip 12. The bottom opening of the tube body 11 is connected to the top opening of the tip 12.

[0115] The sample base 2 is located in the connection between the tube body 11 and the tip 12, and the sample base 2 is provided with a groove 21, the groove opening direction of which is consistent with the top opening direction of the centrifuge tube 1.

[0116] The pressure plate 3 is set on the groove opening side of the groove 21, and the pressure plate 3 is provided with a through hole 31, which is connected to the groove opening 21.

[0117] The steel ball 4 is located inside the tip 12.

[0118] The material cell adhesion ability grading device provided in this embodiment uses a pressure plate to fix the test material in a groove. After the blood mixture enters the centrifuge tube, it can enter the groove through the through hole on the pressure plate and come into contact with the test material. Through the centrifugation process, the interaction between cells in the blood mixture and the test material in the actual separation scenario is simulated. The cell adhesion ability of the test material is graded by the number of residual cells on the test material. This can accurately determine the strength of the material's cell adhesion ability and improve the accuracy of grading the material's cell adhesion ability.

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A method for grading the cell adhesion ability of materials, characterized in that, include: Place multiple steel balls inside the tip of the centrifuge tube; A sample base is placed inside the connection between the body and tip of a centrifuge tube, and the material to be tested is fixed in the groove of the sample base to obtain a first centrifuge tube to be processed, comprising: After the material to be tested is placed in the groove of the sample base, a pressure plate with a through hole is fixed to the groove opening side to fix the material to be tested in the groove; wherein, the through hole is connected to the groove opening; the bottom opening of the tube body is connected to the top opening of the tip; the material to be tested is sheet-like; the groove opening direction is consistent with the top opening direction of the centrifuge tube; a rectangular through hole is opened at the bottom of the groove of the sample base; The blood mixture was poured into the first centrifuge tube to be processed, resulting in a second centrifuge tube to be processed. The second centrifuge tube to be processed is centrifuged, and the material to be observed is obtained based on the material to be tested after centrifugation. The cell adhesion ability level of the test material is determined based on the number of residual cells on the test material.

2. The method for grading the cell adhesion ability of materials according to claim 1, characterized in that, The process of obtaining the material to be observed based on the material to be tested after centrifugation includes: The test material was rinsed with physiological saline after centrifugation to obtain the rinsed material; The cell contact surface of the rinsed material is bonded to a glass slide to obtain the material to be observed; the cell contact surface is the side of the rinsed material that is in direct contact with the blood mixture.

3. The method for grading the cell adhesion ability of materials according to claim 2, characterized in that, Before determining the cell adhesion ability level of the test material based on the number of residual cells on the test material, the following steps are included: The material under observation is observed using a microscope, and multiple residual numbers are obtained based on the number of residual cells on the cell contact surface under multiple different fields of view.

4. The method for grading the cell adhesion ability of materials according to claim 3, characterized in that, The step of determining the cell adhesion ability level of the test material based on the number of residual cells on the test material includes: Based on the number of residues and the morphology of the residues corresponding to the number of residues, determine the multiple cell area values ​​corresponding to the multiple numbers of residues; Calculate the average of the multiple cell area values, and divide the average by the area of ​​the cell contact surface to obtain the percentage of residual cell coverage; The cell adhesion ability level of the test material is determined based on the percentage of residual cell coverage.

5. The method for grading the cell adhesion ability of materials according to claim 4, characterized in that, The step of determining the cell adhesion ability level of the test material based on the residual cell coverage ratio includes: If the residual cell coverage is greater than 70% and less than or equal to 90%, then the cell adhesion ability of the test material is determined to be Level 1. If the residual cell coverage is greater than 50% and less than or equal to 70%, then the cell adhesion ability of the test material is determined to be level two. If the residual cell coverage is greater than 30% and less than or equal to 50%, then the cell adhesion ability of the test material is determined to be level three. If the residual cell coverage is greater than 10% and less than or equal to 30%, then the cell adhesion ability of the test material is determined to be level four. If the residual cell coverage percentage is greater than 0% and less than or equal to 10%, then the cell adhesion ability of the test material is determined to be level five.

6. The method for grading the cell adhesion ability of materials according to claim 1, characterized in that, The process of obtaining the material to be observed based on the material to be tested after centrifugation includes: The cell contact surface of the test material after centrifugation is bonded to a glass slide to obtain the test material; the cell contact surface is the side of the test material after centrifugation that is in direct contact with the blood mixture.

7. The method for grading the cell adhesion ability of materials according to claim 6, characterized in that, The step of determining the cell adhesion ability level of the test material based on the number of residual cells on the test material includes: If the number of residual cells on the material to be observed is zero, then the cell adhesion ability of the material to be tested is determined to be level six.

8. A material cell adhesion ability grading device, used to implement the material cell adhesion ability grading method according to any one of claims 1-7, characterized in that, include: Centrifuge tubes, sample holders, pellets, and steel balls; The centrifuge tube includes a tube body and a tip, wherein the size of the top opening of the tip is larger than the size of the bottom opening of the tip, and the bottom opening of the tube body is connected to the top opening of the tip. The sample base is disposed in the connection between the tube body and the tip, and the sample base is provided with a groove, the groove opening direction being consistent with the top opening direction of the centrifuge tube. The pressure plate is disposed on the groove opening side of the groove, and the pressure plate is provided with a through hole, which is connected to the groove opening; The steel ball is disposed inside the tip.

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