A plant anti-aging composition, its preparation method and application

Abstract

The present invention belongs to the technical field of cosmetic compositions, and particularly relates to a plant anti-aging composition, a preparation method thereof and an application. The present invention provides a plant anti-aging composition, which comprises the following components: kangaroo paw extract, astragalus root extract, kudzu root extract, supramolecular ionic liquid. It is prepared by compounding a variety of plant extracts, which is mild and safe; each component acts on the epidermis, the epidermal-dermal junction layer and the dermis respectively, and has good synergistic effects; a transdermal penetration enhancer for promoting the absorption of anti-aging substances is added, and the anti-aging and wrinkle-removing effects are good.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetic compositions, and particularly relates to a plant anti-aging composition, a preparation method thereof, and an application thereof. Background Art

[0002] Aging is a natural phenomenon that humans must experience in life. The main manifestations of skin aging are: dry skin, skin atrophy, degradation of elastic tissue, thinning of the skin, and the appearance of wrinkles, sagging, and roughness. The causes of aging are very complex and are mainly divided into endogenous aging and exogenous aging. Endogenous aging is caused by internal factors, including: aging, physiological aging, decreased synthesis ability of collagen and elastin in the skin; reduced fluidity of cytoplasm and intercellular matrix. Exogenous aging mainly refers to environmental factors such as ultraviolet radiation, smoking, wind, sun exposure, and exposure to harmful chemicals. Among them, photoaging can cause excessive production of oxygen free radicals, attack membrane lipids, proteins, and DNA, causing oxidative damage, and also up-regulate the expression of genes such as matrix metalloproteinases (MMPs) and down-regulate the expression of collagen, accelerating the formation of skin wrinkles. In order to improve skin wrinkles, people have tried to delay aging through different ways such as increasing collagen in the skin and reducing free radicals in the body.

[0003] At present, most of the anti-aging raw materials on the market use peptides and collagens. These raw materials require cold-chain transportation and low-temperature storage, with demanding requirements and high prices, which are not economically applicable. Moreover, they do not comprehensively delay aging from multiple levels such as the epidermis, the dermo-epidermal junction, and the dermis. There are also some that use plant compositions as anti-aging raw materials, but natural plant active ingredients generally have problems such as difficult dissolution, difficult penetration, and difficult absorption, and the anti-aging effect is minimal. Summary of the Invention

[0004] To solve the problems raised in the above background art, the present invention provides a plant anti-aging composition, a preparation method thereof, and an application thereof. The plant anti-aging composition provided by the present invention not only has simple transportation and storage requirements, is of pure plant origin, and is affordable, but also each component acts on the epidermis, the dermo-epidermal junction, and the dermis respectively, having good synergistic effects. It improves skin wrinkles through multiple synergistic effects such as scavenging free radicals, stimulating the activity of fibroblasts, promoting collagen synthesis, and inhibiting collagen degradation. The combination is reasonable, and a transdermal penetration enhancer for promoting the absorption of anti-aging substances is also added, and the anti-aging and anti-wrinkle effect is good.

[0005] To achieve the above object, the present invention provides the following technical solutions.

[0006] The present invention provides a plant anti-aging composition, comprising the following components: kangaroo paw extract, astragalus extract, pueraria extract, supramolecular ionic liquid.

[0007] The mass ratio of the kangaroo paw extract, astragalus extract, pueraria extract, and supramolecular ionic liquid is (0.4 - 3):(0.3 - 2):(0.2 - 3):(0.1 - 2).

[0008] The supramolecular ionic liquid is betaine - glucosylglycerol.

[0009] The present invention provides a plant anti - aging composition, which is prepared by compounding a variety of plant extracts; in the plant anti - aging composition provided by the present invention, a kangaroo paw extract is added. It is rich in fructose, rutin, and polyphenols, can increase the production of tenascin - X, improve the structure of the dermis layer, and can also stimulate collagen synthesis, enhance elastin synthesis, reduce the appearance of wrinkles and skin laxity. Moreover, it can stimulate the activity of fibroblasts, increase the contractility of fibroblasts, improve skin firmness, reduce laxity, reduce wrinkles, and has a rapid and long - lasting effect.

[0010] In the plant anti - aging composition provided by the present invention, an astragalus extract is added. It is rich in astragaloside, flavonoids, and astragalus polysaccharides, can improve the activity of the enzymatic defense system (SOD\GSH - Px), timely scavenge free radicals, reduce or terminate the chain reaction of free radicals, reduce lipid peroxidation reactions, and can also improve the activity of macrophages, thereby promoting early wound healing and stimulating the proliferation of fibroblasts and keratinocytes. In addition, it also has anti - inflammatory and antibacterial effects.

[0011] In the plant anti - aging composition provided by the present invention, a pueraria extract is added. It is rich in puerarin, daidzin, genistin, daidzein, and genistein, can up - regulate the expression levels of aquaporin 3 (AQP3) and keratin, and can also increase the content of hyaluronic acid (HA), improve the water content of the epidermis, and can also inhibit the expression of matrix metalloproteinase MMP9, reduce the degradation of collagen, promote the synthesis of collagen I and Ⅳ, and reduce skin laxity.

[0012] In the plant anti - aging composition provided by the present invention, a supramolecular ionic liquid, betaine - glucosylglycerol, is added. Betaine has the effects of strengthening the barrier and deeply locking water and moisturizing, and can improve the solubility of active substances; glucosylglycerol has excellent moisturizing ability and good skin permeability. Through supramolecular improvement technology, betaine and glucosylglycerol are closely combined by intermolecular forces to form a supramolecular ionic liquid, achieving the effect of 1 + 1>2. Moreover, based on the amphiphilicity of the supramolecule, it can promote the skin penetration of the three plant extracts, slowly release them, increase the residence time, improve the absorption utilization rate, and effectively improve the transdermal absorption degree.

[0013] By scientifically formulating the above - mentioned plant extracts, the synergistic effect of these three plant extracts in anti - aging can be effectively exerted, and then using the supramolecular ionic liquid to deliver, a significant anti - aging effect can be achieved.

[0014] As a preferred embodiment of the plant anti-aging composition of the present invention, the kangaroo paw extract is two of the kangaroo paw flower extract and the kangaroo paw stem and leaf extract.

[0015] As a preferred embodiment of the plant anti-aging composition of the present invention, the mass ratio of the kangaroo paw flower extract to the kangaroo paw stem and leaf extract is (0.4 - 15):(0.1 - 2).

[0016] Another object of the present invention is to provide a preparation method of a plant anti-aging composition, comprising the following steps:

[0017] S1; Weigh the raw materials of kangaroo paw, astragalus, and kudzu root, pulverize them through a low-temperature pulverizer and sieve (200 mesh) to obtain powder;

[0018] S2: According to a solid-liquid ratio of 1:5 - 50, add an ethanol aqueous solution with a concentration of 30% - 95% to the powder, perform ultrasonic extraction for 0.2h - 1.5h, filter the extract with a 400-mesh filter screen, and perform vacuum concentration at 35°C - 50°C to obtain a concentrated solution. This process is carried out 1 - 2 times;

[0019] S3: Centrifuge the concentrated solution with a disc centrifuge to obtain a centrifugate;

[0020] S4: Further purify the centrifugate with a ceramic membrane and an ultrafiltration membrane to obtain a separated and purified solution;

[0021] S5: Homogenize the supramolecular ionic liquid and the separated and purified solution for 10 - 60 min, filter and sterilize to obtain the plant anti-aging composition.

[0022] Preferably, in step S4, the ceramic membrane selects a ceramic membrane with a pore size of 50 - 500 nm;

[0023] Preferably, in step S4, the ultrafiltration membrane selects a membrane with a molecular weight cut-off of 4000 - 10000 Da.

[0024] Another object of the present invention is to provide an anti-aging emulsion, which, by mass percentage, comprises the following components: 0.5% - 10% of the plant anti-aging composition of the present invention, 0.3% - 13% of a humectant, 0.5% - 3% of an emulsifier, 0.01% - 0.5% of a thickener, 0.01% - 0.5% of a regulator, 0.2 - 0.6% of an antioxidant, 3% - 20% of an oil, and the balance is water.

[0025] As a preferred embodiment of the anti-aging emulsion of the present invention, the humectant is at least one of glycerol, butylene glycol, panthenol, and propylene glycol; the oil is at least one of pentaerythritol tetra(ethylhexanoate), isononyl isononanoate, limnanthes alba seed oil, camellia seed oil, and shea butter.

[0026] Another object of the present invention is to provide the application of the plant anti-aging composition in cosmetics.

[0027] As a preferred embodiment of the application of the plant anti-aging composition in cosmetics, the plant anti-aging composition accounts for 0.5%-10% of the total mass of the cosmetics.

[0028] The composition of the present invention can be used for various different applications to form various different preparations without particular limitation. Specifically, the composition of the present invention can be applied to dosage forms such as essence, water, milk, cream, facial cleanser, facial mask, foundation, etc.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0030] (1) The composition of the present invention is prepared by compounding a variety of plant extracts, without adding preservatives, mild and safe, and can be used for a long time.

[0031] (2) The composition of the present invention has a wide source, low price, simple transportation and storage requirements, and is more economical and affordable.

[0032] (3) The composition of the present invention is compounded from plant extracts and acts on different sites of the epidermis, the dermo-epidermal junction, and the dermis. Anigozanthos extracts act on the dermis, improve the extracellular matrix structure, stimulate the synthesis of collagen and elastin, activate fibroblasts, and increase the contractility of fibroblasts; Astragalus membranaceus extracts act on the dermis and epidermis, scavenge free radicals, reduce lipid peroxidation reactions, stimulate the proliferation of fibroblasts and keratinocytes, and also have certain anti-inflammatory and antibacterial effects; Pueraria lobata extracts act on the dermis, epidermis, and dermo-epidermal junction, inhibit the activity of matrix metalloproteinases, reduce collagen degradation, promote the synthesis of collagen type IV in the dermo-epidermal junction, and can also increase the hyaluronic acid content in the dermis and epidermis, up-regulate the expression levels of aquaporin 3 (AQP3) and keratin, and increase the water content of the epidermis. Each component has a targeted effect on multiple nodes and steps in the process of wrinkle formation, achieving a comprehensive and significant anti-wrinkle effect. Then, a supramolecular ionic liquid (a penetration enhancer) is used to deliver the composite plant composition, which can significantly increase the transdermal absorption amount and permeability, solve the bottleneck problem that has always existed in the industry that active ingredients cannot be well absorbed through the skin barrier, and while promoting the absorption of active ingredients, it can also protect the structure of active ingredients and play a synergistic effect, making the moisturizing and anti-wrinkle effect better. Detailed implementation mode

[0033] The present invention provides a plant anti-aging composition and a preparation method and application thereof. To make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] Unless otherwise specified, the experimental methods used in the examples are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0035] A plant anti-aging composition, composed of the following components in parts by weight, the contents of Examples 1-4 are shown in Table 1 below:

[0036]

[0037] A method for preparing a plant anti-aging composition is completed by the following steps:

[0038] S1; weigh kangaroo paw flower, kangaroo paw stem and leaf, astragalus, and kudzu root according to the above proportions, crush and sieve (200 mesh) with a low-temperature grinder to obtain a powder;

[0039] S2: Add 60% ethanol aqueous solution to the powder at a solid-liquid ratio of 1:10, extract by ultrasonic for 1 h, filter the extract with a 400-mesh filter, and concentrate under reduced pressure at 45°C to obtain a concentrate. This process is repeated twice.

[0040] S3: centrifuging the concentrate using a butterfly centrifuge to obtain a centrifuge;

[0041] S4: the centrifuged liquid is further purified by a ceramic membrane with a pore size of 400 nm and an ultrafiltration membrane with a molecular weight cutoff of 8000 Da to obtain a separated and purified liquid;

[0042] S5: The supramolecular ionic liquid and the separation and purification liquid are homogenized for 40 minutes, filtered and sterilized to obtain a plant anti-aging composition.

[0043] The contents of Comparative Examples 1-13 are shown in Table 2 below by weight:

[0044]

[0045] Preparation method: the same as that of Example 1-4.

[0046] Performance test: The anti-aging compositions prepared in Examples 1-4 and Comparative Examples 1-13 were subjected to the following performance tests:

[0047] Irritation test: The samples of Examples 1-4 and Comparative Examples 1-13 were subjected to irritation tests in accordance with "SN / T 2329-2009 Chicken Embryo Chorioallantoic Membrane Test for Eye Irritation / Corrosion of Cosmetics". The test data obtained were recorded in Table 4, and the test evaluation criteria are shown in Table 3 below:

[0048]

[0049] Table 4 Irritation test results:

[0050]

[0051] Conclusion: It can be seen from Table 4 that all 17 samples showed no / low irritation, had high skin safety, were non-irritating to the human body, and could be used with confidence. However, the total ES of the samples in Comparative Examples 5-6 was slightly higher than that of other samples. The samples in Comparative Examples 5-6 were ground with a single and excessive amount of plants. Therefore, when only one plant is used and the dosage is too high, the irritation will increase.

[0052] Free radical scavenging effect test: The DPPH method was used for detection, and the specific steps are as follows:

[0053] (1) Weigh a certain amount of DPPH and prepare a 0.04 mg / mL DPPH solution with absolute ethanol;

[0054] (2) Take 0.2 mL of the samples of Examples 1-4 and Comparative Examples 1-13 and 3.8 mL of pH 6.8 phosphate buffer solution, add them to 4 mL of DPPH solution, mix well, and place them at 25 °C for 30 min. Then, take the same amount of the sample and measure the absorbance at 517 nm (each sample is measured in parallel three times and the average value is taken). The test results are shown in Table 5 below.

[0055]

[0056] Conclusion: It can be seen from Table 5 that the samples of Examples 1-4 have good free radical scavenging effects. The samples in Comparative Examples 1, 2, 4, 6, and 10 have poor free radical scavenging effects. The samples in Comparative Examples 3, 5, 7, 8, 9, 11, 12, and 13 have good free radical scavenging effects, but are inferior to those of Examples 1-4. This shows that the main function of astragalus extract in the composition is to scavenge free radicals. In addition, the free radical scavenging effect of plant compounding is better than that of single / two plants, indicating that the composition of the present invention has a synergistic effect in scavenging free radicals.

[0057] Anti-aging test: It was characterized by evaluating the inhibition rate of the test samples on elastase. Elastase is mainly secreted by fibroblasts and can degrade elastin in the skin, leading to skin aging. The test method refers to the laboratory method (HMC-WI-028 Elastase Inhibition Rate), and the specific results are shown in Table 6 below.

[0058]

[0059] Conclusion: As can be seen from Table 6, the elastase inhibition rates of Examples 1-4 are relatively high, those of Comparative Examples 3, 5, 9, and 11 are relatively low, and those of Comparative Examples 7, 8, 12, and 13 are relatively high, but lower than those of Examples 1-4. This shows that based on the specific ratio content of each component in the composition, the plant anti-aging composition of the present invention has excellent anti-wrinkle and firming effects.

[0060] The anti-aging compositions prepared from Examples 1-4 and Comparative Examples 7, 8, 12, and 13 were prepared into corresponding anti-aging emulsions, and labeled as Examples 1-4 and Comparative Examples 7, 8, 12, and 13. The percentage content of the emulsion formula is shown in Table 7 below:

[0061]

[0062] The preparation method of the above anti-aging emulsion includes the following steps:

[0063] 1) Add water, humectant, thickener, and antioxidant to the main pot, stir and heat up to 83 ± 2 °C, homogenize at 3000 r / min for 5 min, and disperse evenly;

[0064] 2) Add oil and emulsifier to the auxiliary pot, stir and heat up to 83 ± 2 °C until completely dissolved;

[0065] 3) Pump the material in the auxiliary pot into the main pot, homogenize at 3000 r / min for 7 min for emulsification;

[0066] 4) Add a regulator to adjust the pH value,

[0067] 5) Continue to cool down to 43 ± 2 °C, add the anti-aging composition, and stir for 15 min.

[0068] In order to prove the effects and performance of the products of the present invention, the above anti-aging emulsion was used for the following anti-aging effect tests.

[0069] Anti-aging effect test: 80 subjects aged 36 to 60 years were randomly selected, including 20 males and 60 females; they were randomly divided into 8 groups with 10 people in each group; and they were respectively labeled as Examples 1-4 and Comparative Examples 7, 8, 12, and 13; then, after cleansing their faces in the morning and evening during the test period, the subjects in different groups applied the anti-aging emulsions prepared in Examples 1-4 and Comparative Examples 7, 8, 12, and 13 that matched their groups to their faces after using the toner; before using the product (D0) and 28 days after using the product (D28), the following tests were respectively conducted on each group of subjects: Primos CR was used for skin wrinkle analysis; a skin elasticity measuring instrument Cutometer® MPA580 was used to detect skin elasticity and skin firmness; a skin moisture content measuring instrument Corneometer® CM825 was used to detect the moisture content of the stratum corneum of the skin; the skin wrinkle conditions, skin elasticity, skin firmness, and moisture content of the stratum corneum of the subjects were measured and analyzed, and compared with the data in the D0 period, and the data change rate was calculated. The specific data was recorded in Table 8 below. A smaller skin wrinkle analysis value indicates an improvement in skin wrinkles; a larger skin elasticity measurement value indicates an improvement in skin elasticity, and a smaller skin firmness measurement value indicates an improvement in skin firmness; a larger moisture content measurement value of the stratum corneum of the skin indicates an increase in the moisture content of the stratum corneum of the skin.

[0070]

[0071] Conclusion: As can be seen from Table 8, the wrinkle area and wrinkle depth of the volunteers who tried the emulsions of Examples 1-4 were significantly reduced, and the skin elasticity and firmness were significantly better, indicating that the plant anti-aging composition of the present invention has obvious effects of improving skin firmness, reducing relaxation, and increasing the water content of the stratum corneum. As can be seen from the results of Comparative Example 13, the addition of the plasma liquid significantly promoted the skin penetration of the three plant extracts, making the anti-aging effect more significant. As can be seen from the results of Comparative Examples 7, 8, and 12, the lack of any one of the kangaroo paw flower extract and the kangaroo paw stem and leaf extract or the imbalance of the ratio reduced the synergistic effect, resulting in a poor anti-aging effect.

[0072] Through comparison and analysis of relevant data, it can be known that compared with the comparative examples, the composition prepared in the present invention is composed of a compound of kangaroo paw extract, astragalus extract, pueraria extract, and supramolecular ionic liquid, without adding preservatives, and is mild and safe; each component acts on the epidermis, the epidermal-dermal junction layer, and the dermis respectively, and has a good synergistic effect. It improves skin wrinkles through multiple pathways such as scavenging free radicals, stimulating the activity of fibroblasts, promoting collagen synthesis, and inhibiting collagen degradation. The combination is reasonable, and the supramolecular ionic liquid promotes the skin penetration of plant extracts, effectively improving the transdermal absorption degree. The anti-aging effect is good; in addition, the composition prepared in the present invention has a wide source, low price, simple transportation and storage requirements, and is more economical and practical.

[0073] In the description of this specification, the descriptions referring to terms such as "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0074] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A plant anti-aging composition, characterized in that: The invention comprises the following components: kangaroo paw extract, astragalus extract, kudzu root extract and supramolecular ionic liquid; the mass ratio of the kangaroo paw extract, astragalus extract, kudzu root extract and supramolecular ionic liquid is (0.4-3):(0.3-2):(0.2-3):(0.1-2); the supramolecular ionic liquid is betaine-glycerol glucoside; the kangaroo paw extract is composed of kangaroo paw flower extract and kangaroo paw stem and leaf extract; the mass ratio of the kangaroo paw flower extract and kangaroo paw stem and leaf extract is (0.4-15):(0.1-2).

2. The method for preparing a plant anti-aging composition according to claim 1, characterized in that: The following steps are involved: S1: Weigh kangaroo paw, astragalus root and kudzu root, grind them with a low-temperature grinder and sieve them with 200 mesh to obtain powder; S2: Add 30%-95% ethanol aqueous solution to the powder at a solid-liquid ratio of 1:5-50, perform ultrasonic extraction for 0.2h-1.5h, filter the extract with a 400-mesh filter, and concentrate under reduced pressure at 35°C-50°C to obtain a concentrate. This process is repeated 1-2 times; S3: The concentrate is centrifuged using a butterfly centrifuge to obtain a centrifuge; S4: the centrifuged liquid is further purified by using a 50-500 nm ceramic membrane and an ultrafiltration membrane with a molecular weight cutoff of 4000-10000 Da to obtain a separated and purified liquid; S5: The supramolecular ionic liquid and the separation and purification liquid are homogenized for 10 to 60 minutes, filtered and sterilized to obtain the plant anti-aging composition.

3. An anti-aging lotion, characterized in that: Calculated by mass percentage, it includes the following components: The plant anti-aging composition of claim 1 comprises 0.5%-10%, humectant 0.3%-13%, emulsifier 0.5%-3%, thickener 0.01%-0.5%, regulator 0.01%-0.5%, antioxidant 0.2-0.6%, oil 3%-20%, and the balance is water.

4. The anti-aging lotion according to claim 3, characterized in that: The moisturizer is at least one of glycerin, butylene glycol, panthenol, and propylene glycol; the oil is at least one of pentaerythritol tetra(ethylhexanoate), isononyl isononanoate, meadowfoam seed oil, camellia seed oil, and shea butter.

5. Use of the plant anti-aging composition according to claim 1 in the preparation of cosmetics.

6. The use of the plant anti-aging composition according to claim 5 in the preparation of cosmetics, characterized in that: The plant anti-aging composition accounts for 0.5%-10% of the total weight of the cosmetic.

7. The use of the plant anti-aging composition according to claim 5 in the preparation of cosmetics, characterized in that: The plant anti-aging composition can be used to prepare essence, lotion, facial cream, facial mask and foundation.

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