A repairing film, preparation method and application

Abstract

The present invention belongs to the field of biopharmaceutical materials technology, and specifically relates to a patch with a repairing effect, a preparation method, and applications. The patch comprises the following raw materials: an acidic chitosan solution, a kudzu root powder solution, glycerol, and a polypeptide, wherein the volume ratio of the acidic chitosan solution to the kudzu root powder solution is 1 to 9:9 to 1. The present invention uses chitosan, kudzu root powder, and polypeptide as raw materials, and mixes the polypeptide with a natural composite matrix chitosan / kudzu root powder to prepare a patch with good mechanical properties, excellent biocompatibility, excellent biodegradability, good antibacterial properties, the ability to promote wound healing, and hemostasis. The patch is non-irritating to oral ulcer surfaces, is safe and has no toxic side effects, can adhere to the ulcer surface, and has pain relief, antibacterial, anti-inflammatory, and repair-promoting effects. It can effectively promote ulcer healing, repair and protect wounds from infection, and has excellent biocompatibility and biodegradability. The preparation method is simple and easy to operate.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedical materials, and in particular relates to a film with a repairing effect, a preparation method and an application thereof. Background Art

[0002] Oral mucosal injury is a series of diseases caused by the loss of mucosal integrity due to various reasons, such as recurrent aphthous ulcers, traumatic ulcers, traumatic blood blisters, Behcet's disease, radiation stomatitis, Reiter's syndrome, etc. Oral mucosal ulcers are the most common oral mucosal injury, with a prevalence of more than 25% worldwide, seriously affecting the quality of life of patients. The clinical manifestations of oral mucosal injury are mainly oral mucosal lesions (such as ulcers, erosions, etc.), local bleeding, microbial infection and immune inflammatory response, which may exist alone or in combination depending on the cause of the mucosal injury and the individual differences of the patient. The surface of the ulcer is covered with a yellow pseudomembrane, with a depression in the middle, which is painful and prone to recurrence. The onset period of oral ulcers is usually 10-14 days, and in severe cases, scars may be left. At present, local drug treatment represented by antibiotics, growth factors, fibrin, hyaluronic acid and glucocorticoids is the main method for repairing oral mucosal injury. However, antibiotics and drugs such as glucocorticoids can also cause various side effects, such as gastrointestinal damage, mucosal discoloration, taste disorders, and oral mucosal hypersensitivity. They may also make the ulcer surface more susceptible to anaerobic infection and increase the risk of candidiasis and oral mucosal atrophy.

[0003] Patches are easy to use and can be attached to oral wounds to protect them and reduce pain. They also help maintain a moist oral environment. Patches are easy to use and can be adjusted to release drugs in one or two directions by selecting different preparation processes. They are suitable for patients of all ages and have good patient compliance. Currently, various natural and synthetic materials have been widely used in the preparation of patches, such as polysaccharides, biological products, and nano-self-assembling peptides. However, patches with a single component cannot meet the different needs of ulcer and wound repair. Therefore, an ideal patch dressing should have multiple functions, such as maintaining a moist environment, relieving pain, stopping bleeding, resisting infection, and promoting wound healing.

[0004] Chitosan, a linear polysaccharide derived from the partial N-deacetylation of chitin, exhibits excellent antibacterial properties, film-forming properties, hemostatic properties, and is a biodegradable, biocompatible, and non-toxic polymer. To date, it has been used in the preparation of various biomedical materials, such as membranes, gels, sponges, and nanoparticles. However, the mechanical properties and actual antibacterial performance of chitosan films are not ideal. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a patch with a repairing effect, a preparation method and an application thereof, so as to improve the effect of treating oral ulcers.

[0006] The present invention includes a patch with a repairing effect, which comprises the following raw materials: chitosan acid solution, kudzu root powder solution, glycerin and polypeptide. The volume ratio of the chitosan acid solution to the kudzu root powder solution is 1-9:9-1, preferably 1-5:5-1.

[0007] Preferably, the acid in the chitosan acidic solution is one or more of citric acid, acetic acid, hydrochloric acid, nitric acid, phosphoric acid, ascorbic acid, gallic acid, phytic acid, and lactic acid. More preferably, the acid in the chitosan acidic solution is one or more of citric acid, lactic acid, ascorbic acid, and phytic acid. Preferably, the mass percentage of the acid solution itself in the chitosan acidic solution is preferably 0.5-2%.

[0008] Preferably, the molecular weight of the chitosan is 10 to 500 KDa, preferably 50 to 300 KDa.

[0009] Preferably, the weight concentration of the chitosan is 1-10%, preferably 1%-5%.

[0010] Preferably, the weight concentration of the kudzu root powder solution is 1 to 10%.

[0011] Preferably, the polypeptide is a cationic polypeptide, and the added weight of the polypeptide is 0.001 to 0.1% of the total weight of the chitosan acid solution and the kudzu root powder solution, preferably 0.01 to 0.05%.

[0012] Preferably, the sequence of the polypeptide is one or more of FFRKVLKLIRKI (SEQ ID NO. 1), FFRKVLKLIRKIF (SEQ ID NO. 2) and FFRKVLKLIRKIWR (SEQ ID NO. 3).

[0013] The kudzu root powder solution of the present invention is a solution obtained by mixing kudzu root powder and water, and then heating and stirring the mixture until the mixture is completely gelatinized.

[0014] The chitosan acid solution is a mixture of chitosan and an acid solution, wherein the molecular weight of the chitosan is 200 KDa, the weight concentration of the chitosan is 2%, the acid is ascorbic acid, and the mass concentration of the acid solution is 1%; the volume ratio of the chitosan acid solution to the kudzu root powder solution is 1:1; the weight concentration of the kudzu root powder solution is 2%; and the sequence of the polypeptide is FFRKVLKLIRKIWR.

[0015] The present invention provides a method for preparing a patch with a repairing effect, comprising the following steps: uniformly mixing a chitosan acid solution, a kudzu root powder solution, and glycerin (preferably by stirring at a stirring speed of 100 to 150 rpm for 2 to 4 hours); adding a polypeptide (preferably dissolved in a solvent) to the mixed solution under stirring conditions (preferably at a stirring speed of 100 to 150 rpm for 2 to 4 hours); pouring the mixture into a mold; and drying the mixture to form a film (preferably at room temperature) to obtain the patch with a repairing effect.

[0016] The present invention provides an application of the patch with a repairing effect, wherein the patch is used for preparing medicines or articles for treating ulcers (especially oral ulcers) and repairing wounds.

[0017] The beneficial effect of the present invention is that the present invention uses chitosan, kudzu root powder and polypeptide as raw materials, mixes the polypeptide with the natural composite matrix chitosan / kudzu root powder to prepare a patch with good mechanical properties, excellent biocompatibility, excellent biodegradability, good antibacterial properties, the ability to promote wound healing, and hemostatic function.

[0018] The present invention provides a method for preparing a repairable oral ulcer patch. The method involves loading an antimicrobial polypeptide onto a thin film matrix formed by cross-linking chitosan and kudzu root powder, ultimately yielding a repairable oral ulcer patch with excellent overall performance. The repairable oral ulcer patch prepared by the present invention is non-irritating to the oral ulcer surface, safe, and non-toxic, can adhere to the ulcer surface, and provides pain relief, antibacterial, anti-inflammatory, and repair-promoting effects. It effectively promotes ulcer healing, repairs and protects wounds from infection, and exhibits excellent biocompatibility and biodegradability. Furthermore, the preparation method is simple and easy to operate.

[0019] The present invention completely gelatinizes kudzu root powder, resulting in a more effective treatment than ungelatinized kudzu root powder. While Gegenqinlian decoction is commonly used to treat oral ulcers, the inventors have discovered that using kudzu root powder alone in a patch is more effective than using a combination of kudzu root, qinlian, and quercetin. Kudzu root can increase the elongation and adhesion of the patch. In a patch formulation, kudzu root powder also contains other ingredients beneficial to oral ulcers, making this formulation even more effective in repairing oral ulcers.

[0020] The present invention has found through experiments that chitosan is dissolved with ascorbic acid, chitosan with a larger molecular weight is used, and the sequence of the polypeptide is limited to FFRKVLKLIRKIWR. That is, when the following conditions are defined: the chitosan acid solution is a mixture of chitosan and an acid solution, the molecular weight of the chitosan is 200KDa, the weight concentration of the chitosan is 2%, the acid is ascorbic acid, and the mass concentration of the acid solution is 1%; the volume ratio of the chitosan acid solution to the kudzu root powder solution is 1:1; the weight concentration of the kudzu root powder solution is 2%; and the sequence of the polypeptide is FFRKVLKLIRKIWR. The resulting film is significantly superior to other acids and polypeptides in terms of adhesion time, tensile strength, elongation at break, hemostatic performance, cell survival rate, and ulcer healing rate, and has a better effect on repairing oral ulcers. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is the infrared absorption spectrum of the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. However, the described embodiments are only used to explain the present invention and are not used to limit the scope of the present invention.

[0023] Example 1

[0024] A method for preparing a film with a repairing effect comprises the following steps:

[0025] (1) Preparation of chitosan solution: chitosan (molecular weight 200 kDa, 2 g) was added to 100 g of ascorbic acid aqueous solution (the mass concentration of ascorbic acid in the ascorbic acid aqueous solution was 1.0%) and stirred until completely dissolved;

[0026] (2) Preparation of kudzu root powder solution: 2 g of kudzu root powder was placed in 100 g of water, heated at 80°C, and stirred until completely gelatinized;

[0027] (3) The chitosan solution, the kudzu root powder solution, and 0.6 g of glycerol were stirred and mixed uniformly at a stirring speed of 120 rpm for 3 hours to obtain a chitosan / kudzu root powder mixture;

[0028] (4) Under stirring conditions (stirring speed of 120 rpm; stirring time of 3 hours), the polypeptide solution (the polypeptide sequence is FFRKVLKLIRKIWR, weight is 0.02 g) is dropped into the chitosan / kudzu root powder mixture, stirred evenly and poured into a mold, and dried at room temperature to form a film, thereby obtaining a patch with a repairing effect.

[0029] Example 2

[0030] A method for preparing a film with a repairing effect comprises the following steps:

[0031] (1) Preparation of chitosan solution: chitosan (molecular weight 50 kDa, 4 g) was added to 100 g of acetic acid aqueous solution (the mass concentration of acetic acid in the acetic acid aqueous solution was 2.0%) and stirred until completely dissolved;

[0032] (2) Preparation of kudzu root powder solution: 4 g of kudzu root powder was placed in 100 g of water, heated at 80°C, and stirred until completely gelatinized;

[0033] (3) The chitosan solution, the kudzu root powder solution, and 1.6 g of glycerol were stirred and mixed uniformly at a stirring speed of 120 rpm for 3 hours to obtain a chitosan / kudzu root powder mixture;

[0034] (4) Under stirring conditions (stirring speed of 120 rpm; stirring time of 3 hours), the polypeptide solution (the polypeptide sequence is FFRKVLKLIRKI, weight is 0.06 g) is dropped into the chitosan / kudzu root powder mixture, stirred evenly and poured into a mold, and dried at room temperature to form a film, thereby obtaining a patch with a repairing effect.

[0035] Example 3

[0036] Compared with Example 1, the difference is that in step (2), the kudzu root powder solution is prepared by adding kudzu root powder (0.5 g) to 50 g of water and stirring until completely dissolved. Other steps are the same as in Example 1.

[0037] Comparative Example 1

[0038] Compared with Example 1, the difference is that in step (2), a kudzu root and scutellaria root solution is prepared instead of the kudzu root powder solution. The kudzu root and scutellaria root solution is prepared by decocting 2 g of kudzu root, 0.8 g of roasted liquorice root, 1.2 g of scutellaria root, and 1.2 g of coptis root with water and concentrating to 100 g. At the same time, in step (4), no polypeptide solution is added. Other steps are the same as in Example 1.

[0039] Comparative Example 2

[0040] Compared with Example 1, the difference is that in step (2), a quercetin solution is prepared instead of the kudzu root powder solution, and in step (4), no polypeptide solution is added. The quercetin solution is prepared by adding 2 g of quercetin to anhydrous ethanol and dissolving it to obtain a quercetin solution.

[0041] Comparative Example 3

[0042] Compared with Example 1, the difference is that in step (4), no polypeptide solution is added, that is, an equal amount of water is used instead of the polypeptide solution and dripped into the chitosan / kudzu root powder mixture.

[0043] Comparative Example 4

[0044] Compared with Example 1, the difference is that no kudzu root powder solution and polypeptide solution are added. Other aspects are the same as Example 1.

[0045] Comparative Example 5

[0046] Compared with Example 1, the difference is that no chitosan solution and polypeptide solution are added. Other steps are the same as in Example 1.

[0047] The following tests were performed to verify the quality evaluation, antibacterial properties, hemostatic properties, biocompatibility and in vivo repair-promoting effects of each film material of the present invention.

[0048] 1. Quality evaluation of film materials

[0049] (1) Film-forming property evaluation: The evaluation was based on the appearance. The specific scoring criteria were: uniform color, smooth surface, no bubbles (10 points); uniform color, smooth surface, 1-5 bubbles (8 points); uniform color, rough surface, 1-5 bubbles (6 points); uniform color, rough surface, >5 bubbles (4 points); uneven color, rough surface, >5 bubbles (2 points). See Table 1.

[0050] (2) Film-forming property: The film is evaluated based on the area of ​​film removal. The specific scoring criteria are: the film can be completely removed (10 points); the film is completely removed in an area of ​​80-100% (8 points); the film is completely removed in an area of ​​60-80% (6 points); the film is completely removed in an area of ​​40-60% (4 points); and the film is completely removed in an area of ​​20-40% (2 points). See Table 1.

[0051] (3) Film swelling and adhesion performance test

[0052] 1) Artificial saliva formulation: 10 g sodium carboxymethylcellulose, 0.9 g sodium chloride, 1.2 g potassium chloride, 0.052 g magnesium chloride, 30 g sorbitol, 200 mL of a 0.053% g / mL solution of calcium phosphate (ρ), 10 mL of a 0.2% g / mL solution of sodium phosphate (ρ), 0.33 g paraben. Add distilled water to 1000 mL.

[0053] 2) Determination of swelling and adhesion duration. Moisten the film with artificial saliva and apply it to the inner wall of a beaker. Record the time from wetting to adhesion. Then, slowly add 100 mL of artificial saliva. Place in a constant-temperature water bath (32°C) and stir at 100 rpm / min. Record the time until the film breaks or falls off, which is the adhesion duration. See Table 1.

[0054] (4) The mechanical properties of the film, including tensile strength and elongation at break, were tested using a WDW-10D universal testing machine. The test conditions were: the film was cut into 1 × 8 cm rectangles; a 100 N sensor was selected, the initial gripper spacing was 30 mm, and the probe speed was 20 mm / min. See Table 2.

[0055] Table 1 Quality evaluation of film materials

[0056]

[0057] Table 2 Mechanical properties of film materials

[0058]

[0059] As shown in Table 1, the chitosan / kudzu root powder composite film exhibits superior film-forming, film-lifting, adhesion, and mechanical properties compared to chitosan and kudzu root powder films. In particular, the chitosan / kudzu root powder composite film with the addition of antimicrobial peptides exhibits significant improvements in tensile strength and elongation after exercise. Furthermore, the addition of kudzu root powder significantly impacts the composite film's film-forming, film-lifting, adhesion, and mechanical properties, indicating an interaction between kudzu root powder and chitosan, forming a well-defined molecular network structure.

[0060] 2. Evaluation of antibacterial properties of film materials

[0061] The antibacterial properties of the film materials were evaluated by plate colony counting method. Different film materials were sterilized by UV and placed in a 48-well plate, and then 500 μL (1×10 6 CFU mL -1 ) bacterial solution and co-cultured in a shaker at 37°C at 2000 rpm for 16 hours. The co-culture solution was then serially diluted, and 20 μL of the diluted solution was aspirated and dropped onto an agar plate. The plate was evenly spread using a sterile applicator and incubated in a 37°C incubator for 24 hours. Finally, colony counts were performed on the plates, and the inhibition rate was calculated. The results are shown in Table 3. As shown in Table 3, the chitosan / kudzu root powder composite film exhibits moderate antibacterial properties, which are significantly enhanced with the addition of antimicrobial peptides. It exhibits significant inhibitory activity against Staphylococcus aureus, Escherichia coli, and Candida albicans, and this inhibition rate increases with the addition of antimicrobial peptides.

[0062] Table 3 Antibacterial properties of film materials

[0063]

[0064] 3. Evaluation of hemostatic performance of film materials

[0065] (1) Blood coagulation index: Equal amounts of the film materials were placed in centrifuge tubes and preheated in a 37°C constant temperature incubator for 5 minutes. Then, 50 μL of recalcified whole blood solution (the concentration of CaCl2 in blood is 10 mM) was added to the film materials. After a blood clot was formed for a certain period of time, 5.0 mL of PBS was added to release the uncoagulated blood. The absorbance of the supernatant at 540 nm was measured using a multifunctional microplate reader. The whole blood coagulation index (BCI) of the film (%) = As / Ac × 100 (where As is the absorbance value of the sample group; Ac is the absorbance value of the negative control group).

[0066] (2) Blood coagulation time: Place equal amounts of film material in a centrifuge tube and preheat in a 37°C incubator for 5 minutes. Then, add 100 μL of fresh whole blood solution containing an anticoagulant and a CaCl2 (10 mM) solution. The timer is started from the time the CaCl2 solution is added. Tilt the tube every 30 seconds in the early stages. When coagulation is about to begin, tilt the tube every 10 seconds until the blood is completely coagulated. The results are shown in Table 4.

[0067] As shown in Table 4, compared with chitosan film and kudzu root powder film, the chitosan / kudzu root powder composite film has better procoagulant performance, and after adding antimicrobial peptides, its blood coagulation index is significantly reduced and the blood coagulation time is significantly shortened.

[0068] Table 4 Hemostatic properties of film materials

[0069]

[0070]

[0071] 4. Biocompatibility analysis of the film

[0072] (1) Hemolytic activity: Weigh equal amounts of the membrane material, add 500 μL of PBS solution, then add 500 μL of a 2% red blood cell suspension, mix well, and incubate at 37°C for 1 h. The mixture is then centrifuged at 1,000 × g for 15 min, and the absorbance of the supernatant at 570 nm is measured using a multifunctional microplate reader to calculate the hemolytic rate. 1% Triton X-100 was used as a positive control, and PBS was used as a negative control. The results are shown in Table 5.

[0073] (2) Cut the film material into discs with a diameter of 3 mm and a thickness of 2 mm and sterilize them under UV light for 15 min. L929 cells were cultured at a density of 1.0 × 10 4Cells were seeded at a density of 100 cells / well in a 96-well plate. After incubation at 37°C, 5% CO₂ until the cell density reached 80-90%, the sponge samples equilibrated in complete DMEM were gently placed into the 96-well plate and incubated at 37°C, 5% CO₂ for 24 hours. Cell viability was then determined using the CCK-8 colorimetric assay. The results are shown in Table 5.

[0074] Table 5 Hemolytic activity and cytotoxicity of the film materials

[0075]

[0076] Table 5 shows that while all chitosan / kudzu root powder composite films exhibited varying hemolysis rates, all were below 5%, indicating that all film materials possessed good hemocompatibility. Furthermore, none of the chitosan / kudzu root powder composite films exhibited cytotoxicity against L929 cells, and the composite film materials containing antimicrobial peptides even exhibited a moderate proliferation-promoting effect, demonstrating that the antimicrobial peptides were evenly distributed throughout the chitosan / kudzu root powder composite films and exhibited excellent sustained-release properties.

[0077] 5. Evaluation of the repair-promoting effect of film materials in vivo

[0078] (1) Establishment of a chemical injury model of gingival mucosal ulcer in rats: Rats were anesthetized for 3 min using 3% (w / v) sodium pentobarbital (intraperitoneal injection, 30 mg / kg). After successful anesthesia, the rats were fixed, the gingival mucosa of the rats was cleaned with saline, and a circular filter paper with a diameter of 5 mm was soaked with 20 μL of 70% acetic acid solution. The filter paper was then placed on the gingival mucosa of the rats for 60 s to form an ulcer, and the ulcer was rinsed with physiological saline to completely remove the residual acetic acid. After two days of continuous monitoring, on the third day, the rats were divided into 7 groups: control group, Example 1 group, Example 2 group, Example 3 group, and Comparative Example 1-5 groups.

[0079] (2) Treatment process: Treatment was started after the oral mucosal ulcer was rinsed with normal saline. The patch material was applied to the ulcer surface. The mucosal ulcer site of each group of rats was photographed every other day and the ulcer area was calculated. After 2 days of treatment, the surface of the gingival ulcer was wiped back and forth several times with a sterile cotton swab, and then placed in a centrifuge tube of MH broth. The centrifuge tube containing the cotton swab was then placed in an ultrasonic instrument for 5 minutes. Then, the solution was diluted in sequence, and 20 μL was drawn from the diluted solution and dropped onto the agar plate. The solution was evenly spread on the plate with a sterile applicator and then placed in a 37°C incubator for 24 hours. Finally, the plate colony count was performed and the antibacterial rate was calculated. After 6 days of treatment, the animals were euthanized and the gingival mucosa around the ulcer was collected. The ulcer area was calculated and compared with the wound area on day 0.

[0080] (3) HE staining: Rat gingival mucosa samples were fixed in 4% paraformaldehyde solution, then embedded in paraffin, and the paraffin blocks were cut into 5 μm thick sections. The sections were stained with HE to evaluate the inflammatory status of the ulcer tissue.

[0081] Table 6 Food intake of rats

[0082]

[0083] Table 7 Rat ulcer healing rate

[0084]

[0085]

[0086] Table 8 Antibacterial properties of the film on ulcer rats

[0087]

[0088] Table 9 Wound inflammation in rats with ulcers after 6 days of treatment

[0089]

[0090] As shown in Table 6, the food intake of ulcer mice decreased after successful modeling compared with before modeling, and the food intake of rats in each drug-treated group also decreased, but increased compared with the blank group. The food intake of the group with antimicrobial peptide film increased most significantly, and basically returned to the level before film creation after 8 days of treatment, which was significantly recovered compared with 2 days of drug administration.

[0091] After successful ulcer modeling in mice, distinct ulcers were observed. The ulcer surface was slightly concave, grayish-white in color, with red, swollen, and tender edges. Table 7 shows that after two days of treatment, the ulcer area in all groups treated with the patch decreased, while the ulcer area in the control group increased. After four days of treatment, the ulcer area in all groups decreased, particularly in the group treated with the antimicrobial peptide patch, where the ulcer healing rate exceeded 60%. After six days of treatment, the ulcers in most rats treated with the antimicrobial peptide patch were completely healed.

[0092] As shown in Table 8, after 2 days of treatment, all the patch treatment groups had a certain anti-infection effect on ulcers, especially the patch group with added antimicrobial peptides, the antibacterial rate reached 100%, indicating that the antimicrobial peptide patch also has a good antibacterial effect in vivo.

[0093] As shown in Table 8, after 6 days of treatment, there were still a large number of inflammatory cells infiltrating in the ulcer tissue of the control group. The proportion of inflammatory cells in the ulcer tissue of each patch treatment group was significantly lower than that of the control group, especially the patch group with added antimicrobial peptides, in which the proportion of inflammatory cells was only 4-6%.

[0094] 6. Infrared spectrum analysis of film materials

[0095] Chitosan powder (CS), kudzu powder (PL), antimicrobial peptide HX-12C powder (AMP), and freeze-dried powder of a mixed solution of chitosan powder, kudzu powder, and antimicrobial peptide HX-12C were pressed into tablets with potassium bromide. Their structures were then examined by Nicolet iS10 Fourier transform infrared absorption spectroscopy (FT-IR). The results are shown in Figure 2. Figure 1 In the spectrum of antimicrobial peptide HX-12C, the wavelengths of 3000-3500 cm -1 There is a broad peak at 2970cm, which is the characteristic absorption band of NH and OH stretching vibration. -1 The band at 1670 cm is attributed to the stretching vibration of CH. -1 and 1540cm -1 Two characteristic peaks were observed at 3000-3500 cm-1, namely, amide I band (antisymmetric stretching vibration absorption peak of C=O) and amide II band (bending vibration absorption peak of -NH). -1 There are also characteristic absorption bands of -OH stretching and -NH stretching vibration at 1710 cm -1 and 1600cm -1 Two characteristic absorption peaks, amide I band and amide II band, were also observed at 1400 cm -1 The absorption peak centered at 1140 cm is the symmetrical stretching and bending deformation vibration absorption band of CH. -1 The peak at 3440 cm is the COC stretching vibration peak. After adding antimicrobial peptide HX-12C, it shows an absorption peak similar to that of chitosan powder alone, without any additional peaks, indicating that no covalent bond is formed. However, compared with the spectrum of antimicrobial peptide HX-12C, the amide I band and amide II band both move to lower wavenumbers, indicating that there is a hydrogen bond interaction between antimicrobial peptide HX-12C and chitosan. In the spectrum of Pueraria root powder, the peak at 3440 cm -1 The band centered at 1630 cm is mainly caused by -OH stretching vibration. -1 The absorption peak is the C=O stretching vibration peak, 1150cm -1 The peak of COC stretching vibration is at 1670cm. After adding antimicrobial peptide HX-12C, the spectrum shows that the -OH absorption peak moves to a lower wave number, which may be the result of the interaction of intermolecular hydrogen bonds between the two. -1 ) was significantly weakened, indicating that there was an interaction between Pueraria root powder and antimicrobial peptide HX-12C. -1 and 1020cm -1A CO absorption peak appeared at 100 nm. In the spectrum of the chitosan powder, kudzu root powder, and antimicrobial peptide HX-12C mixture, no new peaks appeared, but the absorption peaks for amide II, CH stretching vibration, and -OH stretching vibration all shifted slightly. These results indicate a strong molecular interaction between chitosan, kudzu root powder, and antimicrobial peptide HX-12C.

[0096] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of protection of the present application is limited to these examples. In line with the present application, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of different aspects of one or more embodiments of the present application as described above, which are not provided in detail for the sake of simplicity.

[0097] The one or more embodiments of this application are intended to encompass all such substitutions, modifications, and variations that fall within the broad scope of this application. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of one or more embodiments of this application should be included in the scope of protection of this application.

Claims

1. A film with a repairing effect, characterized in that: The method comprises the following raw materials: chitosan acid solution, kudzu root powder solution, glycerin and polypeptide, wherein the volume ratio of the chitosan acid solution to the kudzu root powder solution is 1-9:9-1; In the chitosan acidic solution, the acid is one or more of citric acid, acetic acid, hydrochloric acid, nitric acid, phosphoric acid, ascorbic acid, gallic acid, phytic acid, and lactic acid; The sequence of the polypeptide is one or more of FFRKVLKLIRKI, FFRKVLKLIRKIF and FFRKVLKLIRKIWR.

2. The repair film according to claim 1, wherein: In the chitosan acidic solution, the acid is one or more of citric acid, lactic acid, ascorbic acid and phytic acid.

3. The repair film according to any one of claims 1 to 2, wherein: The molecular weight of the chitosan is 10 to 500 KDa; the weight concentration of the chitosan is 1 to 10%.

4. The repair film according to any one of claims 1 to 2, characterized in that: The weight concentration of the kudzu root powder solution is 1-10%.

5. The repair film according to any one of claims 1 to 2, characterized in that: The polypeptide is a cationic polypeptide, and the added weight of the polypeptide is 0.001-0.1% of the total weight of the chitosan acid solution and the kudzu root powder solution.

6. The repair film according to any one of claims 1 to 2, wherein: The chitosan acid solution is a mixture of chitosan and an acid solution, wherein the molecular weight of the chitosan is 200 KDa, the weight concentration of the chitosan is 2%, the acid is ascorbic acid, and the mass concentration of the acid solution is 1%; the volume ratio of the chitosan acid solution to the kudzu root powder solution is 1:1; the weight concentration of the kudzu root powder solution is 2%; and the sequence of the polypeptide is FFRKVLKLIRKIWR.

7. A method for preparing a repair film according to any one of claims 1 to 6, characterized in that: Chitosan acid solution, kudzu root powder solution and glycerin are mixed evenly, and polypeptide is added to the mixed solution under stirring, poured into a mold, and dried to form a film to obtain a patch with a repairing effect.

8. An application of the repair film according to any one of claims 1 to 6, characterized in that: The patch is used for preparing medicines or articles for treating ulcers and repairing wounds.

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