An aphid repellent

Abstract

This invention provides one use of borneol as an aphid repellent, specifically for the control of tobacco aphids. This invention also provides a method for repelling tobacco aphids by using borneol as a repellent. After analyzing the volatile components of tobacco infected with cucumber mosaic virus, this invention discovered that borneol has aphid-repelling effect and can be used to prepare repellents, thus providing a new compound for aphid control.

Description

Technical Field

[0001] This invention belongs to the field of green pest control technology, and in particular relates to a repellent for the tobacco aphid (peach aphid). Background Technology

[0002] The tobacco aphid, also known as the peach aphid, is one of the most widely distributed aphids in the world, found in Asia, North America, Europe, and Africa. It is distributed throughout all provinces of China. Besides damaging tobacco, the tobacco aphid feeds on more than 170 species of plants, including cruciferous and legume families, making it a typical polyphagous pest. Adults and nymphs of the tobacco aphid damage host plants by piercing and sucking their flesh, reducing yields. Their honeydew excretions can also induce plant diseases such as sooty mold. Furthermore, the tobacco aphid transmits various cucumber mosaic virus (CMV) and potato virus Y (PVY) diseases, causing even more severe crop losses.

[0003] Currently, chemical pesticides are mostly used to control aphids, which can easily lead to pesticide residues and environmental pollution. Developing repellents can provide support for green control technologies. Summary of the Invention

[0004] The purpose of this invention is to provide a use of borneol as an aphid repellent, specifically for the control of tobacco aphids.

[0005] The present invention first provides one use of borneol, which is as a repellent for tobacco aphids;

[0006] This invention provides a method for repelling tobacco aphids, which uses borneol as a repellent to control tobacco aphids;

[0007] Preferably, in the method, the concentration of borneol is 0.1–10 μg / μL;

[0008] The present invention also provides a liquid formulation for repelling tobacco aphids, wherein the formulation is prepared by adding borneol to a solvent;

[0009] Furthermore, the concentration of borneol in the formulation is between 0.1 and 10 μg / μL.

[0010] As a specific example, the solvent used is paraffin.

[0011] Furthermore, the liquid formulation also contains one or more of the following: methyl salicylate, linalool, terpinene-4-ol, and geranylacetone.

[0012] This invention, after analyzing the volatile components of tobacco infected with cucumber mosaic virus (CMV), discovered that borneol has the effect of repelling tobacco aphids and can be used to prepare repellents, thus providing a new compound for aphid control. Attached Figure Description

[0013] Figure 1 : Illustration of the repellent effects of five compounds on aphids. Detailed Implementation

[0014] During the experiment, it was discovered that infection of tobacco with cucumber mosaic virus (CMV) altered the tactic behavior of the vector tobacco aphid. Analysis of the volatile components of CMV-infected tobacco revealed that borneol has a repellent effect on tobacco aphids and can be used to prepare repellents.

[0015] The present invention will now be described in detail with reference to the embodiments and accompanying drawings.

[0016] Example 1: Determination that Borneol has the effect of repelling tobacco aphids.

[0017] 1. Selection behavior of tobacco aphids on tobacco plants infected with Cucumber Mosaic Virus (CMV).

[0018] The selection test of tobacco aphids on different susceptible tobacco plants was conducted using a Y-type olfactory instrument. The tobacco plants selected for the Y-type olfactory instrument were mid-stage CMV infection and healthy tobacco plants. During the experiment, a single adult aphid (starved for 1 hour) was placed at the outlet of the Y-type tube, and its behavior was observed and recorded. If the tobacco aphid entered about half of a certain odor arm within 10 minutes and stayed for more than 30 seconds, it was recorded as selective behavior towards the tobacco plant on that side; if it did not enter any arm, it was recorded as no selection. The odor tube position was changed for every 5 aphids tested, and each group of experiments was repeated 100 times.

[0019] The results showed that 56 aphids chose healthy tobacco plants, 11 aphids chose CMV tobacco plants, and the remaining aphids showed no obvious reaction, indicating that CMV tobacco plants have a repellent effect on tobacco aphids, which may be related to the volatile components they release.

[0020] 2. Detection of the types of volatile substances

[0021] Tobacco volatiles were collected using dynamic headspace collection. The tobacco roots were sealed with aluminum foil to prevent soil gas volatilization. The entire tobacco plant was then sealed in a gas collection bag and left to stand for 5 hours. Air carrying the plant's aroma flowed out from the valve of the collection bag, passed through a glass tube containing 60 mg of Tenax TA adsorbent, and was then extracted by an atmospheric sampler at a flow rate of 150 mL / min. The air then flowed out of the sampler and back into the collection bag through the valve, forming a cycle. Each collection was performed for 12 hours. The extracted compounds were eluted with 600 μL of n-hexane into a sample vial, concentrated to 100 μL using a nitrogen blowdown apparatus, and stored at -20°C for chemical analysis. The tested tobacco plants included those in the mid-stage of CMV infection and healthy plants at the same stage, with five replicates for each host plant at different stages. GC-MS was used for the analysis and identification of tobacco volatiles.

[0022] The results of the volatile component analysis are shown in Table 1. The results indicate that after CMV contamination of tobacco, the content of many volatile compounds increased significantly, including borneol, linalool, and methyl salicylate; the content of trans-β-ocimene did not change much, while the content of 3,3-dimethylhexanal decreased significantly.

[0023] Table 1: Main types of volatile substances in CMV-infected tobacco plants

[0024]

[0025] Example 2: Testing the repellent effects of different compounds on aphids

[0026] Borneol, benzyl alcohol, linalool, methyl salicylate, terpinene-4-ol, and geranylacetone were selected and their repellent effects were determined using a Y-type olfactometer at a concentration of 0.1 μg / μL. Paraffin oil solvent was used as a control, and 30 aphids were tested each time. The repellency rate was calculated as (CK-T) / (CK+T)×100%. The results showed that the repellency rates of borneol, terpinene-4-ol, and geranylacetone were 33.33%, 31.03%, and 28.00%, respectively, indicating significant repellent effects. Benzyl alcohol, linalool, and methyl salicylate had slightly lower repellent effects, all below 20%. Trans-β-ocimene not only had no repellent effect on aphids but also had an attractant effect. The compound 3,3-dimethylhexanal also had almost no repellent effect.

[0027] Furthermore, the results showed that for the same compound, different concentrations resulted in varying repellent effects. Using paraffin as a solvent, the repellent effect of borneol increased with increasing concentration within the range of 0.1–10 μg / μL, with 10 μg / μL being the optimal concentration. Concentrations exceeding 10 μg / μL showed no significant increase in effectiveness and also increased costs.

[0028] In summary, this invention confirms that borneol has a repellent effect on aphids and can be used to prepare corresponding products.

Claims

1. One use of borneol, characterized in that, The stated use is as a repellent for tobacco aphids.

2. A method for repelling tobacco aphids, characterized in that, The method described involves using borneol as a repellent to control tobacco aphids.

3. The method as described in claim 2, characterized in that, The concentration of borneol used in the method is 0.1~10 μg / μL.

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