Composition for promoting brown rice germination, application, germinated brown rice and preparation method thereof

Abstract

The invention belongs to the field of food, and discloses a composition for promoting brown rice germination, including bamboo leaf water extract and dodder extract. The composition includes bamboo leaf water extract and dodder extract. The main function of bamboo leaf water extract is antibacterial. Bamboo leaves contain a large amount of flavonoids, phenolic acid, anthraquinone compounds, etc., which have a strong antibacterial effect and have a strong inhibitory effect on several common contaminating bacteria in food. The dodder extract is rich in ascorbic acid, tocopherol, phenols, flavonoids, amino acids, mineral nutrients and plant hormones; low doses of dodder extract can improve the germination vitality of brown rice under water stress, and seeds grown under non-stress conditions also have similar improvements. High concentrations of phenols and flavonoids may improve germination and seedling emergence; at the same time, low doses of dodder extract can improve the antibacterial performance of bamboo leaf water extract; the two work together to significantly improve the germination rate of brown rice and control mildew; at the same time, the invention also provides an application based on the composition, germinated brown rice and a preparation method thereof.

Description

Technical Field

[0001] The invention belongs to the field of food, and particularly relates to a composition for promoting brown rice germination, application, germinated brown rice and a preparation method thereof. Background Art

[0002] Brown rice has a poor taste due to the lack of fine processing, takes a long time to cook, and is difficult to chew, but it contains a large amount of dietary fiber, minerals, vitamins, essential amino acids and other nutrients. When brown rice is placed under certain temperature and humidity conditions to induce germination, its taste will be greatly improved, the tissue will soften, and it will be easy to cook. The proteases and amylases in it will be activated and released, changing from a bound state to a free state, and the content of reducing sugars and amino acids will increase. At the same time, a variety of biologically active substances beneficial to human health will be generated, such as γ-aminobutyric acid, γ-glutamic acid, phenolic acid, etc. In the process of preparing germinated brown rice, soaking treatment is usually required. During this process, the number of bacteria and molds increases, and their metabolites will hinder the germination of brown rice and affect the quality of the germinated brown rice. Therefore, antibacterial treatment is usually carried out before brown rice germinates. Currently, NaClO, H 2 O 2 , O 3 , ultraviolet rays, etc., these chemical agents are more or less toxic, and there are also problems such as unstable antibacterial effects, residual chemical antibacterial substances, damage to the brown rice itself, and affecting the germination rate of brown rice. Among them, the residual chemical agents have a huge impact on the food safety of germinated brown rice.

[0003] CN107912708A discloses a method for processing sprouted-pregelatinized nutritious, healthy and palatable brown rice, which further discloses that the brown rice is fermented with an alcohol-soluble extract of bamboo leaves;

[0004] After verification by the applicant, we found that compared with the control group using distilled water, the germination rate of brown rice fermented with bamboo leaf water extract increased by 10% to 12%, and the mildew phenomenon of brown rice was reduced after the brown rice was fermented with bamboo leaf water extract.

[0005] However, the above germination rate and the control of corresponding mildew phenomenon did not reach the expected level. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a composition for promoting brown rice germination. The composition comprises bamboo leaf water extract and dodder seed extract. The main function of the bamboo leaf water extract is antibacterial. Bamboo leaves contain a large amount of flavonoids, phenolic acid, anthraquinone compounds, etc., which have a strong antibacterial effect and have a strong inhibitory effect on several common contaminating bacteria in food. The dodder seed extract is rich in ascorbic acid, tocopherol, phenols, flavonoids, amino acids, mineral nutrients and plant hormones. A low dose of dodder seed extract can improve the germination vitality of brown rice under water stress, and seeds grown under non-stress conditions also have similar improvements. High concentrations of phenols and flavonoids may improve germination and seedling emergence. At the same time, a low dose of dodder seed extract can improve the antibacterial performance of the bamboo leaf water extract. The two work together to significantly improve the germination rate of brown rice and control mildew at the same time.

[0007] Meanwhile, the invention also provides application of the composition, germinated brown rice and a preparation method thereof.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] A composition for promoting brown rice germination comprises bamboo leaf water extract and dodder seed extract.

[0010] In the above composition for promoting brown rice germination, the bamboo leaf water extract is a solution or powder obtained by water extracting 1 part by weight of bamboo leaves;

[0011] The dodder seed extract is equivalent to 1‰ to 1% of the weight of bamboo leaves.

[0012] Preferably, the Cuscuta seed extract is equivalent to 1‰ to 5‰ of the weight of bamboo leaves.

[0013] In the above composition for promoting brown rice germination, the dodder seed extract is a water-soluble extract, and its effective ingredients include ascorbic acid, tocopherol and flavonoids.

[0014] The composition for promoting brown rice germination further comprises a water-soluble grapefruit peel extract;

[0015] The weight ratio of the water-soluble grapefruit peel extract to the dodder seed extract is 1 to 3:10.

[0016] Water-soluble grapefruit peel extract contains pectin oligosaccharides, naringin and other ingredients. By adding water-soluble grapefruit peel extract, the seed germination rate can be further improved, but which specific ingredient plays a role is not yet known.

[0017] In the above composition for promoting brown rice germination, the preparation method of the bamboo leaf water extract is:

[0018] After crushing the bamboo leaves, soak them in hot water for a period of time to dissolve the water-soluble components in the bamboo leaves.

[0019] In the above composition for promoting brown rice germination, the amount of water used is not less than 20 times the weight of the bamboo leaves, the extraction time is not less than 10 minutes, and the extraction temperature is not less than 95°C.

[0020] Meanwhile, the invention also discloses the use of any of the above compositions in preparing germinated brown rice.

[0021] Meanwhile, the invention also discloses a method for germinating brown rice, which comprises using any of the above compositions to soak the brown rice in a germination machine for 3 to 9 hours; then washing the brown rice with water and placing it in a moist environment for germination.

[0022] In the above germination method, the soaking temperature of brown rice is 32±2°C; the germination temperature is 30±2°C; and the germination time is not less than 40h.

[0023] Finally, the invention also discloses germinated brown rice, which is prepared by the germination method as described above.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The composition of the invention comprises bamboo leaf water extract and dodder seed extract. The main function of the bamboo leaf water extract is to inhibit bacteria. Bamboo leaves contain a large amount of flavonoids, phenolic acid, anthraquinone compounds and the like, and have a strong antibacterial effect, and have a strong inhibitory effect on several common contaminating bacteria in food. The dodder seed extract is rich in ascorbic acid, tocopherol, phenols, flavonoids, amino acids, mineral nutrients and plant hormones. A low dose of the dodder seed extract can improve the germination vitality of brown rice under water stress, and seeds grown under non-stress conditions also have similar improvements. High concentrations of phenols and flavonoids may improve germination and seedling emergence. At the same time, a low dose of the dodder seed extract can improve the antibacterial performance of the bamboo leaf water extract. The two work together to significantly improve the germination rate of brown rice and control mildew.

[0026] After the water-soluble grapefruit peel extract is further added to the composition of the present invention, the germination rate is further improved, and its specific mechanism is not clear yet.

[0027] After the brown rice is treated with the composition of the present invention, conventional germination methods can be used for germination, and the operation is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a photo of brown rice before germination;

[0029] Figure 2 This is a photo of brown rice after germination;

[0030] Figure 3 A photo of moldy brown rice. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0032] Sources of reagents and equipment:

[0033] Bamboo leaves: Bozhou Chujianhuakai E-commerce Co., Ltd.

[0034] Brown rice: organic bran rice, Jilin Province Sanhe Farm Co., Ltd.;

[0035] Cuscuta australis extract: Shandong Tianjiu Biotechnology Co., Ltd.;

[0036] Water-soluble grapefruit peel extract: Shaanxi Tianxingjian Biochemical Technology Co., Ltd.;

[0037] Germination machine: Hangzhou Baobo Plastic Technology Co., Ltd.

[0038] Example 1

[0039] Step 1: Weigh 1g of bamboo leaves and crush them in a beaker, add 50ml of 100℃ hot water, soak for 30min, cool to room temperature, and then filter to obtain antibacterial bamboo leaf water;

[0040] Step 2: adding a dodder extract equivalent to 1 wt‰ of the weight of the bamboo leaves to the antibacterial bamboo leaf water (preparing a dodder extract solution with a concentration of 5 wt% in advance) to obtain a composite solution;

[0041] Step 3: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0042] Step 4: Add the composition solution to the above four culture dishes until the composition solution covers the brown rice, and place them in a sprouting machine and soak at 32° C. for 6 hours;

[0043] Step 5: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30℃ to germinate for 41 hours.

[0044] Count the amount of moldy and sprouted brown rice.

[0045] Example 2

[0046] The method is substantially the same as Example 1, except that the amount of the Cuscuta seeds extract is equivalent to 3wt‰ of the weight of the bamboo leaves.

[0047] Example 3

[0048] The method is substantially the same as Example 1, except that the amount of the Cuscuta seeds extract is equivalent to 5wt‰ of the weight of the bamboo leaves.

[0049] Example 4

[0050] The method is substantially the same as Example 1, except that the amount of the Cuscuta seeds extract is equivalent to 10wt‰ of the weight of the bamboo leaves.

[0051] Example 5

[0052] The method is substantially the same as Example 1, except that in step 1, the amount of water is 100 ml and the soaking time is 60 min.

[0053] Example 6

[0054] Step 1: Weigh 1g of bamboo leaves and crush them in a beaker, add 50ml of 100℃ hot water, soak for 30min, cool to room temperature, and then filter to obtain antibacterial bamboo leaf water;

[0055] Step 2: adding a dodder extract equivalent to 5wt‰ of the weight of the bamboo leaves (pre-prepared with a dodder extract solution with a concentration of 5wt%) and a water-soluble grapefruit peel extract equivalent to 10wt% of the weight of the dodder extract (pre-prepared with a water-soluble grapefruit peel extract solution with a concentration of 5wt%) to the antibacterial bamboo leaf water to obtain a composite solution;

[0056] Step 3: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0057] Step 4: Add the composition solution to the above four culture dishes until the composition solution covers the brown rice, and place them in a sprouting machine and soak at 32° C. for 6 hours;

[0058] Step 5: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30℃ to germinate for 41 hours.

[0059] Count the amount of moldy and sprouted brown rice.

[0060] Example 7

[0061] The method is substantially the same as Example 6, except that the amount of the water-soluble grapefruit peel extract is equivalent to 30% of the weight of the dodder seed extract.

[0062] Comparative Example 1

[0063] Step 1: Weigh 1g of bamboo leaves and crush them in a beaker, add 50ml of 100℃ hot water, soak for 30min, cool to room temperature, and then filter to obtain antibacterial bamboo leaf water;

[0064] Step 2: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0065] Step 3: Add antibacterial bamboo leaf water to the above 4 culture dishes, until the antibacterial bamboo leaf water covers the brown rice, and place them in a sprouting machine at 32°C and soak for 6 hours;

[0066] Step 4: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30°C to germinate for 41 hours.

[0067] Count the amount of moldy and sprouted brown rice.

[0068] Comparative Example 2

[0069] Step 1: Take 50 ml of distilled water, add 0.1 ml of dodder extract solution (concentration 5 wt%) to obtain dodder solution;

[0070] Step 2: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0071] Step 3: Add dodder solution to the above 4 culture dishes until the dodder solution covers the brown rice, and place them in a germination machine and soak at 32°C for 6 hours;

[0072] Step 4: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30°C to germinate for 41 hours.

[0073] Count the amount of moldy and sprouted brown rice.

[0074] Comparative Example 3

[0075] Step 1: Take 50 ml of distilled water, add 0.03 ml of water-soluble grapefruit peel extract solution (concentration 5 wt%), to obtain a grapefruit peel extract solution;

[0076] Step 2: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0077] Step 3: Add grapefruit peel extract solution to the above 4 culture dishes until the grapefruit peel extract solution covers the brown rice, and place in a sprouting machine and soak at 32° C. for 6 hours;

[0078] Step 4: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30°C to germinate for 41 hours.

[0079] Count the amount of moldy and sprouted brown rice.

[0080] Blank Group

[0081] Step 2: Peel 200 plump brown rice grains by hand, wash them with distilled water, divide them into 4 groups, place them in 4 culture dishes, and label them ①, ②, ③, ④;

[0082] Step 3: Add distilled water to the above 4 culture dishes until the brown rice is submerged in the distilled water, and place them in a sprouting machine at 32°C and soak for 6 hours;

[0083] Step 4: After 6 hours, pour out the soaking liquid, wash the brown rice with distilled water, cover the 4 groups with gauze, place the brown rice on the gauze, add distilled water to keep it moist, and place it in a germination machine at 30°C to germinate for 41 hours.

[0084] Count the amount of moldy and sprouted brown rice.

[0085] The data of the above examples, comparative examples and blank groups were statistically analyzed, and the results are shown in Table 1.

[0086] Table 1 Brown rice germination results data table

[0087] Number of germinations / grain Number of mold / particles Example 1 127 44 Example 2 139 38 Example 3 148 35 Example 4 133 37 Example 5 120 34 Example 6 155 36 Example 7 163 33 Comparative Example 1 114 51 Comparative Example 2 106 63 Comparative Example 3 94 61 Blank Group 92 65

[0088] Figure 1 This is a photo of brown rice raw material. Figure 2 This is a photo of brown rice after germination. Figure 3 This is a photo of germinated brown rice that is partially moldy.

[0089] Result analysis:

[0090] 1. It can be seen from Examples 1-5 that the antibacterial bamboo leaf water and the dodder extract are used for matching. Compared with Comparative Example 1, the germination rate is significantly improved and the mildew rate is effectively controlled, indicating that the dodder extract can not only improve the antibacterial properties of the bamboo leaf water extract, but also reduce the number of mildewed brown rice. At the same time, a low dose of the dodder extract can improve the germination activity of brown rice under water stress. When the dosage of the dodder extract exceeds 5‰, its ability to promote germination activity is relatively suppressed, but the antibacterial ability is not weakened.

[0091] 2. It can be seen from Examples 3, 6 and 7 that when the water-soluble grapefruit peel extract is additionally added, the germination rate is improved, but the mildew rate does not change significantly, indicating that the water-soluble grapefruit peel extract does not have a significant effect on promoting the antibacterial function of the antibacterial bamboo leaf water.

[0092] 3. It can be seen from Comparative Examples 1-3 that the water-soluble grapefruit peel extract alone has no obvious effect on the control of germination rate or mildew rate. The dodder extract alone can improve the germination rate of seeds under water stress, but has no obvious promotion on the antibacterial ability of seeds.

[0093] The applicant declares that the present invention illustrates the process method of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned process steps, and it does not mean that the present invention must rely on the above-mentioned process steps to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials selected by the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A composition for promoting brown rice germination, characterized in that: It is composed of bamboo leaf water extract, dodder seed extract, and water-soluble grapefruit peel extract; The preparation method of the bamboo leaf water extract is: 1 part by weight of bamboo leaves were crushed and soaked in 50 parts by weight of hot water for 30 minutes, cooled to room temperature and filtered to obtain a bamboo leaf water extract; The dodder seed extract is equivalent to 1‰ to 10‰ of the weight of bamboo leaves; the dodder seed extract is a water-soluble extract, and its effective ingredients include ascorbic acid, tocopherol, and flavonoids; the weight ratio of the water-soluble grapefruit peel extract to the dodder seed extract is 1 to 3:

10.

2. The composition for promoting brown rice germination according to claim 1, characterized in that The extraction temperature of the bamboo leaf water extract during the extraction process is not lower than 95°C.

3. Use of the composition as claimed in claim 1 or 2 in preparing germinated brown rice.

4. A method for germinating brown rice, characterized in that: The composition as claimed in claim 1 or 2 is used to soak brown rice in a sprouting machine for 3 to 9 hours; then the brown rice is washed with water and placed in a moist environment for sprouting.

5. The germination method according to claim 4, characterized in that: The soaking temperature of brown rice is 32±2℃; the germination temperature is 30±2℃; the germination time is not less than 40h.

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