A method and system for real-time wide-field color imaging using scattered light

Abstract

The present application relates to a kind of real-time wide field of view color imaging method and system using scattered light.The method: different visible light band coherent laser is uniformly mixed to obtain multi-wavelength mixed parallel light;Scattered light formed after mixed parallel light passes through scattering medium is simultaneously carried out RGB three channels individual feedback type wavefront shaping, complete the pre-correction of mixed parallel light after scattering medium;The sample to be imaged is placed between mixed parallel light output and scattering medium, according to the imaging field of view range adjustment sample to be imaged position, obtain the sample image of RGB three channels and carry out fusion to obtain the color image of sample to be imaged, realize the real-time wide field of view color imaging of scattered light.The present application only needs to correct scattered light in advance, realizes the imaging of any transparent sample in the imaging range under the condition that scattering medium does not change or slightly changes, has real-time;While introducing optical memory effect, imaging field of view is no longer limited to optical axis, can realize wide field of view imaging.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of computational optical imaging, and particularly relates to a method and system for real-time wide-field color imaging using scattered light. BACKGROUND

[0002] Optical imaging through scattering media has great research value in the fields of biological tissue imaging, space-based imaging and fog imaging. The non-uniform scattering medium causes the spherical wavefront distortion of each point source due to the random refractive index change, resulting in image blurring and limiting optical observation. According to the scattering times of photons in the scattering medium, the photons are divided into the following three categories: ballistic photons, snake photons and scattered photons. The ballistic photons propagate in the same direction as the direction of the photons entering the scattering medium, and are approximately considered as not scattered. The snake photons are mainly near-axis transmission, and the photons are slightly scattered. The scattered photons propagate in a completely different direction from the incident light direction, and have undergone multiple scattering. The ballistic light retains the original object information because it does not collide with the scattering medium; while the scattered light loses most of the information and often appears as noise in imaging. Existing scattering medium imaging methods such as optical coherence tomography, confocal microscopy and adaptive optical imaging technology can achieve imaging through scattering media to a certain extent. However, they rely on non-scattered "ballistic" photons, and the ballistic photons decay exponentially in depth, so the imaging effect is limited in highly scattering turbid media. Although the scattered light is often regarded as imaging noise, it still carries sample information and occupies a large proportion in highly scattering media. Therefore, how to utilize the scattered light to realize real-time imaging is of great significance for high scattering medium imaging. SUMMARY

[0003] The purpose of the present application is to provide a method and system for real-time wide-field color imaging using scattered light. The method first corrects the transmission characteristics of scattered light in the scattering medium, and then realizes imaging of any transparent sample within the imaging range. Since only the scattered light needs to be corrected in advance, the imaging of any transparent sample within the imaging range can be realized under the condition that the scattering medium remains unchanged or changes slightly, thus having real-time performance. At the same time, due to the introduction of optical memory effect, the imaging field of view is no longer limited to the optical axis, so wide-field imaging can be realized.

[0004] To achieve the above purpose, the technical scheme of the present application is as follows: a method for real-time wide-field color imaging using scattered light, characterized in that it comprises:

[0005] uniformly mixing coherent lasers of different visible light bands to obtain mixed parallel light of multiple wavelengths;

[0006] The feedback type wavefront shaping system is used to simultaneously perform single feedback type wavefront shaping on the scattered light formed by the mixed parallel light after passing through the scattering medium, a focusing image of the incident light is obtained on a color industrial camera, and pre-correction of the mixed parallel light after passing through the scattering medium is completed; after the pre-correction, the scattering medium is converted into a “scattering lens” with a focal length of R, that is, a wide-field “scattering microscope”;

[0007] The optimal amplitude compensation diagram obtained according to the pre-correction is loaded on the digital micromirror device, the sample to be imaged is placed between the mixed parallel light output and the scattering medium, the position of the sample to be imaged is adjusted according to the imaging field of view range, the sample images of the RGB three channels are obtained, the sample images of the RGB three channels are fused to obtain a color image of the sample to be imaged, and real-time wide-field color imaging of the scattered light is realized.

[0008] In an embodiment of the present application, the different visible light wave bands are coherent lasers in red, green and blue wave bands, and the mixed parallel light of multiple wavelengths is obtained by mixing the coherent lasers in the red, green and blue wave bands.

[0009] In an embodiment of the present application, the scattered light formed by the mixed parallel light after passing through the scattering medium is simultaneously subjected to single feedback type wavefront shaping of the RGB three channels, and the pre-correction of the mixed parallel light after passing through the scattering medium is completed.

[0010] The scattered light formed by the mixed parallel light of multiple wavelengths obtained by mixing the coherent lasers in the red, green and blue wave bands is subjected to wavefront shaping, the light intensity information of the color scattered light R, G and B three channels is simultaneously obtained by using a color industrial camera, and real-time feedback is performed by taking the light intensity value of the region of interest set in the color industrial camera as a feedback signal, so that high-intensity light intensity focusing of the region of interest is realized, that is, the pre-correction of the mixed parallel light after passing through the scattering medium is completed.

[0011] In an embodiment of the present application, the wavefront shaping is realized by a digital micromirror device, which performs pure amplitude modulation on the scattered light formed by the mixed parallel light of multiple wavelengths obtained by mixing the coherent lasers in the red, green and blue wave bands, and uses an intelligent optimization algorithm based on binary amplitude modulation (including continuous sequence optimization algorithm, particle swarm optimization algorithm, genetic algorithm, greedy algorithm, bat algorithm and the like, and the genetic algorithm is preferably adopted in the present example) to perform wavefront shaping.

[0012] In an embodiment of the present application, the scattering medium is a steady-state scattering medium such as ground glass or a dynamic scattering medium such as water mist.

[0013] In an embodiment of the present application, the specific implementation of adjusting the position of the sample to be imaged according to the imaging field of view range is as follows:

[0014] The image intensity on the camera plane is measured when the tiny sample is at different positions away from the original optimized point source, and this is used as the light intensity of the tiny sample offset from the original optimized point source position. The measured light intensity data is then fitted with the theoretical calculation formula of the optical memory effect to obtain the imaging field of view, where the theoretical calculation formula of the optical memory effect is C(qL) = [qL / sinh(qL)], q = 2πΔθ / λ, λ is the wavelength of the incident light, Δθ is the rotation angle, and L is the thickness of the scattering medium; then the position of the sample to be imaged is adjusted so that the sample to be imaged is near the optical axis and does not exceed the imaging field of view.

[0015] In one embodiment of the present invention, the sample to be imaged is a transparent color sample or a grayscale sample, and has a length ranging from 2 to 5 mm, a width ranging from 2 to 5 mm, and a thickness ranging from 0.001 to 1 mm.

[0016] The present invention also provides a real-time wide-field color imaging system using scattered light, comprising a multi-wavelength mixed light source module and a wavefront shaping and imaging module; wherein,

[0017] The multi-wavelength mixed light source module is used to uniformly mix coherent lasers within the three wavelength bands of red, green and blue to obtain multi-wavelength mixed parallel light;

[0018] The wavefront shaping and imaging module is used to:

[0019] (1) The scattered light formed after the mixed parallel light passes through the scattering medium is simultaneously subjected to separate feedback wavefront shaping of the three channels of RGB, thereby completing the pre-correction of the mixed parallel light after passing through the scattering medium;

[0020] (2) The position of the sample to be imaged, which is placed between the mixed parallel light output and the scattering medium, is adjusted according to the imaging field of view, and sample images of the three RGB channels are obtained. The sample images of the three RGB channels are fused to obtain a color image of the sample to be imaged, thereby realizing real-time wide-field color imaging of scattered light.

[0021] In one embodiment of the present invention, the multi-wavelength hybrid light source module includes a light source module, a beam mixing module, and a beam expansion module; wherein,

[0022] The light source module consists of a blue band coherent laser 1, a green band coherent laser device 2, and a red band coherent laser 3, which are used to provide coherent light within the three bands of blue, green, and red;

[0023] The beam mixing module is used to achieve uniform mixing of the coherent light of the blue, green and red bands of the light source module to obtain mixed parallel light;

[0024] The beam expansion module is used to achieve amplification and collimation of mixed parallel light.

[0025] In an embodiment of the present application, the wavefront shaping and imaging module comprises a uniform light beam module, a sample to be imaged, a scattering medium, a 4F module, a light field modulation module, an image receiving module, a data integration processing module; wherein,

[0026] The uniform light beam module is used to convert the collimated input mixed parallel light beam into an output light beam with uniform intensity;

[0027] The scattering medium is used to distort the mixed parallel light wavefront to obtain scattered light;

[0028] The 4F module is used to zoom in and image to the light field modulation module and the image receiving module;

[0029] The light field modulation module (using a digital micromirror device) is used to perform pure amplitude wavefront shaping on the scattered light whose wavefront is distorted after passing through the scattering medium, to realize the recovery of the wavefront;

[0030] The image receiving module (using a color CMOS camera) is used to receive image information of R, G and B channels respectively;

[0031] The data integration processing module is used to use an intelligent optimization algorithm based on binary amplitude modulation to perform wavefront shaping on the scattered light, and to perform data processing on the collected image information and fuse the image information of the three channels using an algorithm to obtain a color image.

[0032] The wavefront shaping and imaging module realizes the imaging process as follows: 1) pre-correction of the scattering medium. First, without placing the sample to be tested, the mixed parallel light passes through the scattering medium and is imaged to the digital micromirror device by the 4F module. After modulation by the digital micromirror device, the speckle image is collected by the color CMOS camera, and the wavefront shaping algorithm (i.e. an intelligent optimization algorithm based on binary amplitude modulation) is used to pre-correct the wavefront changes introduced by the scattering medium. 2) Wide field color imaging. Then, the sample to be imaged is placed in the target area at the rear end of the mixed parallel light source and the front end of the scattering medium, and transparent color sample imaging is performed.

[0033] Compared with the prior art, the present application has the following beneficial effects: the present application provides a real-time wide-field color imaging method and system using scattered light, which has real-time performance, only needs to correct the scattered light in advance, and can realize imaging of any transparent sample in the imaging range under the condition that the scattering medium is unchanged or slightly changed; at the same time, due to the introduction of optical memory effect, the imaging field is no longer limited to the optical axis, and has the ability of wide-field imaging. The method and system are different from the dependence of traditional imaging technology on ballistic light, can perform wavefront shaping on scattered light, effectively overcome the problem of wavefront distortion of light caused by multiple scattering in the scattering medium, and through the mixing of red, green and blue band coherent lasers, not only can better pre-correct and improve the imaging quality, but also can obtain the color imaging effect of the sample to be imaged. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 is a method implementation flowchart of an embodiment of the present application.

[0035] Figure 2 is a system structure principle diagram of an embodiment of the present application.

[0036] Figure 3 is a speckle pattern of mixed parallel light after passing through a scattering medium in an embodiment of the present application.

[0037] Figure 4 is a result diagram of pre-correcting in an embodiment of the present application. DETAILED DESCRIPTION

[0038] The technical solutions of the present application will be specifically described below with reference to the drawings.

[0039] It should be pointed out that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0040] It should be noted that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is intended to include the plural form, and in addition, it should be understood that when the terms "comprise" and / or "include" are used in the specification, they indicate the presence of a feature, step, operation, device, component and / or combination thereof.

[0041] As Figure 1As shown, this embodiment provides a method for real-time wide-field color imaging using scattered light. First, coherent lasers from different visible light bands are uniformly mixed to produce multi-wavelength mixed parallel light. Then, a feedback wavefront shaping system simultaneously performs separate feedback wavefront shaping on the three RGB channels of the mixed parallel light after it passes through a scattering medium. A focused image of the incident light is obtained on a color industrial camera, completing the pre-correction of the mixed parallel light after it passes through the scattering medium. After pre-correction, the scattering medium and the optical components in front of the image plane effectively form a "scattering lens" with a focal length of R, i.e., a wide-field "scattering microscope." Next, the optimal amplitude compensation map obtained based on the pre-correction is loaded onto a digital micromirror device. The sample to be imaged is placed behind the mixed parallel light source and in front of the scattering medium. The position of the sample to be imaged is adjusted according to the imaging field of view. A color CMOS camera is used to capture image information of the sample in the three RGB channels. These three channel sample images are then fused to obtain a color image of the sample to be imaged, ultimately achieving real-time wide-field color imaging of scattered light.

[0042] like Figure 2As shown, this embodiment provides a real-time wide-field color imaging system using scattered light for implementing the above-mentioned method, including a multi-wavelength mixing light module and a wavefront shaping and imaging module. The multi-wavelength mixing light module is composed of a light source module (composed of 1-3), a beam mixing module (composed of 4-7), and a beam expansion module (composed of 8-9). The light source module is composed of a blue band coherent laser 1, a green band coherent laser device 2, and a red band coherent laser 3, and is used to provide coherent light in the three bands of blue, green, and red. The beam mixing module is composed of a wide-range wide-band reflector 4 and 7 in the visible light band, and a long-wavelength dichroic spectrometer 5 and 6, and is used to achieve uniform mixing of the coherent light in the three bands of blue, green, and red of the light source module. The beam expansion module is composed of a continuously variable magnification beam expander 8 and a convex lens 9, and is used to achieve amplification and collimation of the mixed light. The wavefront shaping and imaging module consists of a uniform beam module (composed of 10-11), a sample to be imaged 12, a scattering medium module 13, a 4F module (composed of 14-15, 17-18), a light field control module 16, an image receiving module 19, and a data integration processing module 20. The uniform beam module consists of a rotating optical diffuser diffraction optical element 10 and an aperture 11, which is used to convert the collimated input mixed light beam into an output light beam with uniform intensity. The scattering medium module is composed of a steady-state scattering medium such as frosted glass, which is used to distort the wavefront and obtain scattered light. The 4F module is composed of two 4F systems consisting of a microscope objective 14 and lens 15, and lens 17 and lens 18, which are used to perform variable magnification imaging to the light field control module 16 and the image receiving module 19. The light field control module 16 is composed of a digital micromirror device, which is used to perform pure amplitude wavefront shaping on the scattered light whose wavefront is distorted after passing through the scattering medium, thereby realizing wavefront recovery. The image receiving module is composed of a color CMOS industrial camera 19, which is used to receive image information from the three channels R, G, and B. The data integration and processing module is composed of a computer 20, which uses an intelligent optimization algorithm based on binary amplitude modulation to call the digital micromirror device and obtain the optimal amplitude mask. It also processes the collected image information and uses an algorithm to fuse the image information from the three channels to obtain a color image.

[0043] The implementation process of the present invention will be further described below with reference to a specific embodiment.

[0044] Figure 1 The following are the steps for implementing the method for real-time wide-field color imaging using scattered light provided in this embodiment, including:

[0045] 1) Utilize the construction of a real-time wide-field color imaging system using scattered light to obtain multi-wavelength mixed parallel light.

[0046] 2) The scattered light formed after the mixed light passes through the scattering medium is subjected to three-channel wavefront shaping to achieve pre-correction.

[0047] 3) Calculate the imaging field of view and adjust the position of the sample to be imaged according to the imaging field of view.

[0048] 4) The sample images of the three channels R, G, and B are fused to obtain a color image of the sample to be imaged.

[0049] Among them, the construction of a real-time wide-field color imaging system using scattered light includes a multi-wavelength hybrid light module and a wavefront shaping and imaging module, such as Figure 2 As shown:

[0050] Multi-wavelength hybrid optical module, specific operation: The light source module preferably uses a 635nm semiconductor red laser as the red band coherent laser 3, a 532nm semiconductor green laser as the green band coherent laser 2, and a 420nm semiconductor blue laser as the blue band coherent laser 1. First, turn on the 635nm semiconductor red laser 3 to emit collimated red coherent parallel light, which passes through the visible light band wide-range wide-band reflector 4 placed horizontally at 45° and then reflects at 90°. Then, it passes through the long-wavelength pass dichroic spectrometer 5 placed horizontally at 45° (transmits 584-800nm ​​band, reflects 380-550nm band) and transmits. At this time, turn on the 532nm semiconductor green laser 2 to emit collimated green coherent parallel light, which passes through the long-wavelength pass dichroic spectrometer 5 placed horizontally at 45° (transmits 584-800nm ​​band, reflects 380-550nm band) and evenly mixes with the transmitted red coherent parallel light. After passing through a long-wavelength dichroic beam splitter plate 6 (transmitting the 505-800nm ​​band and reflecting the 380-470nm band) positioned horizontally at a 45° angle, the beam is transmitted. At this point, the 420nm semiconductor blue laser 1 is turned on to emit collimated blue coherent parallel light. This is reflected by the long-wavelength dichroic beam splitter plate 6 (transmitting the 505-800nm ​​band and reflecting the 380-470nm band) positioned horizontally at a 45° angle, and evenly mixed with the transmitted red and green mixed parallel light, achieving uniform mixing of the red, green, and blue light in the beam mixing module. The mixed light then passes through a visible light wide-range wide-band reflector 7 positioned horizontally at a 45° angle, and is reflected at a 90° angle to the beam expansion module. The mixed light passes through a 5x to 10x continuously variable magnification beam expander 8, which changes its beam diameter. The expanded mixed light is then collimated by a convex lens 9, completing amplification and collimation, before entering the uniform beam module.

[0051] The wavefront shaping and imaging module has the following specific operations: the collimated and amplified mixed light beam is converted into an output light beam with uniform intensity by a rotating optical diffuser diffractive optical element 10, and then passes through a variable aperture 11 to improve the quality of the mixed light beam and obtain a mixed light beam of appropriate size; the mixed light beam passes through a steady-state scattering medium 13, such as 220-mesh ground glass, to obtain scattered light, which is amplified and imaged on the mirror surface of a digital micromirror device (DMD, DLP7000) 16 after passing through a 4F system consisting of a 10x objective lens 14 (0.25NA, f=10.2mm) and a convex lens 15 (f=150mm). The computer 20 controls the digital micromirror device. In order to facilitate area division and save time, the 768×768 pixel area in the middle part of the DMD is used to divide it into several modulation units. After being modulated by the digital micromirror device, the mixed light beam is collimated by a 4F system consisting of a convex lens 17 (f=200mm) and a convex lens 18 (f=200mm) and imaged on a color complementary metal oxide semiconductor (CCM) 16. A color CMOS camera receives image information of three channels, R, G, and B, respectively, and transmits the information to the computer 20.

[0052] Furthermore, the scattered light formed after the mixed light passes through the scattering medium is subjected to wavefront shaping of three channels to achieve pre-correction. The specific operation is as follows: after completing the construction of the experimental system, the scattered light formed after the mixed light of red, green and blue wavelengths passes through the scattering medium is subjected to wavefront shaping by combining the genetic algorithm based on binary amplitude modulation. The light intensity information of the three channels R, G and B of the colored scattered light is obtained simultaneously by using a color industrial camera, and the light intensity value of the area of ​​interest set in the color industrial camera is used as the feedback signal for real-time feedback to achieve high-intensity light focusing in the area of ​​interest, thus completing the pre-correction. In the experiment, the amplitude mask image generated by the genetic algorithm was first binarized according to the control principle of the digital micromirror device; secondly, the initial parameters of the genetic algorithm based on binary amplitude modulation were set as 40 initial populations, 20 subpopulations generated in each iteration, initial mutation rate 0.1, final mutation rate 0.0025, attenuation factor 80, and maximum number of iterations 500; during the optimization process, the genetic algorithm based on binary amplitude modulation generated 40 initial amplitude mask images, which were loaded into the digital micromirror device in turn. The color CMOS camera collected the modulated light field information with millisecond response and transmitted it to the computer. The light intensity of the region of interest in the three channels of R, G, and B was used as the fitness function. The generated amplitude mask images were sorted, the parent and parent generations with higher light intensity in the region of interest were selected, the offspring amplitude mask images were cross-generated, mutated, and loaded into the digital micromirror device, and this cycle was repeated; finally, the optimal amplitude mask image was obtained, and the pre-correction of the mixed light beam was completed. Figure 3The speckle pattern of the mixed light beam after passing through the scattering medium is shown, Figure 4 The focusing pattern of the mixed light beam after passing through the scattering medium is shown.

[0053] Further, the imaging field of view is calculated, and the position of the sample to be imaged is adjusted according to the imaging field of view range. Specifically, first, the field of view (FOV) in the wide-field imaging of scattered light is determined by the optical memory effect. In order to determine the imaging field of view of the experimental system, the image intensity on the camera plane is measured when the 50um pinhole is at different positions from the original optimized point source, which is used as the light intensity offset from the original optimized point source position. Then, the measured light intensity is normalized, and a graph of the distance corresponding to the optical axis is drawn. The result is fitted with the theoretical formula of the memory effect in the scattering sample, and the imaging field of view range is obtained. The theoretical calculation formula of the optical memory effect is C(qL)=[qL / sinh(qL)], where q=2πΔθ / λ, λ is the wavelength of the incident light, Δθ is the rotation angle, and L is the thickness of the scattering medium. Finally, since the imaging field of view limits the spatial size of the sample to be measured, a transparent color sample with a suitable spatial size is selected, and the transparent color sample to be measured is placed at the center of the optical axis at the front end of the scattering medium and within the imaging field of view range, so as to transfer the sample information. The sample is a color sample, and the color image of the sample can also be obtained by using real-time wide-field color imaging.

[0054] Further, the image information of the R, G, and B channels of the sample is obtained, and a color image is obtained by algorithm fusion. Specifically, when obtaining the image information of the sample, the R, G, and B channels of the color CMOS camera are used to simultaneously obtain image information corresponding to different wavelengths. An image fusion algorithm based on the RGB three channels is used, such as a weighted average algorithm, a maximum and minimum fusion algorithm, a Laplacian pyramid fusion algorithm, a wavelet transform fusion algorithm, and a linear weighted fusion algorithm, to obtain better color sample image information.

[0055] The above is only a preferred embodiment of the present application, and is not intended to limit the present application in other forms. Any person skilled in the art can modify or change the above disclosed technical content to obtain equivalent embodiments. However, any simple modification, equivalent change and modification of the above embodiments based on the technical essence of the present application, without departing from the technical solution of the present application, still falls within the protection scope of the present application.

Claims

1. A method for real-time wide-field color imaging using scattered light, characterized in that: include: Evenly mix coherent lasers of different visible light bands to obtain multi-wavelength mixed parallel light; The scattered light formed after the mixed parallel light passes through the scattering medium is simultaneously subjected to separate feedback wavefront shaping of the three RGB channels to complete the pre-correction of the mixed parallel light after passing through the scattering medium; The sample to be imaged is placed between the mixed parallel light output and the scattering medium, and the position of the sample to be imaged is adjusted according to the imaging field of view. The sample images of the three RGB channels are acquired, and the sample images of the three RGB channels are fused to obtain a color image of the sample to be imaged, thereby realizing real-time wide-field color imaging of scattered light. The coherent lasers in different visible light bands are coherent lasers in the range of red, green and blue bands respectively, and multi-wavelength mixed parallel light is obtained by mixing the coherent lasers in the range of red, green and blue bands; The specific implementation method of simultaneously performing independent feedback wavefront shaping of the three RGB channels on the scattered light formed after the mixed parallel light passes through the scattering medium to complete the pre-correction of the mixed parallel light after passing through the scattering medium is as follows: Wavefront shaping is performed on the scattered light formed by mixing multi-wavelength parallel light obtained by coherent lasers in the red, green, and blue bands after passing through a scattering medium. A color industrial camera is used to simultaneously obtain the light intensity information of the three channels (R, G, and B) of the colored scattered light. The light intensity value of the area of ​​interest set in the color industrial camera is used as the feedback signal for real-time feedback, achieving high-intensity light focusing in the area of ​​interest, that is, completing the pre-correction of the mixed parallel light after passing through the scattering medium. The wavefront shaping is achieved through a digital micromirror device, which performs pure amplitude modulation on the scattered light formed by mixing multi-wavelength mixed parallel light obtained by mixing coherent lasers in the red, green and blue bands and passing it through a scattering medium, and uses an intelligent optimization algorithm based on binary amplitude modulation to perform wavefront shaping.

2. The method for real-time wide-field color imaging using scattered light according to claim 1, characterized in that: The scattering medium includes a steady-state scattering medium and a dynamic scattering medium.

3. The method for real-time wide-field color imaging using scattered light according to claim 1, characterized in that: The specific implementation method of adjusting the position of the sample to be imaged according to the imaging field range is: The image intensity on the camera plane is measured when the tiny sample is at different positions away from the original optimized point source, and this is used as the light intensity of the tiny sample offset from the original optimized point source position. The measured light intensity data is then fitted with the theoretical calculation formula of the optical memory effect to obtain the imaging field of view, where the theoretical calculation formula of the optical memory effect is C(qL) = [qL / sinh(qL)], q = 2πΔθ / λ, λ is the wavelength of the incident light, Δθ is the rotation angle, and L is the thickness of the scattering medium; then the position of the sample to be imaged is adjusted so that the sample to be imaged is near the optical axis and does not exceed the imaging field of view.

4. The method for real-time wide-field color imaging using scattered light according to claim 1, characterized in that: The sample to be imaged is a transparent color sample or a grayscale sample, and has a length range of 2-5 mm, a width range of 2-5 mm, and a thickness range of 0.001-1 mm.

5. A real-time wide-field color imaging system using scattered light, characterized in that: It includes a multi-wavelength hybrid light source module and a wavefront shaping and imaging module; wherein, The multi-wavelength mixed light source module is used to uniformly mix coherent lasers within the three wavelength bands of red, green and blue to obtain multi-wavelength mixed parallel light; The wavefront shaping and imaging module is used to: (1) The scattered light formed after the mixed parallel light passes through the scattering medium is simultaneously subjected to separate feedback wavefront shaping of the three channels of RGB, thereby completing the pre-correction of the mixed parallel light after passing through the scattering medium; (2) adjusting the position of the sample to be imaged, which is placed between the mixed parallel light output and the scattering medium, according to the imaging field of view, obtaining sample images of the three RGB channels, and fusing the sample images of the three RGB channels to obtain a color image of the sample to be imaged, thereby realizing real-time wide-field color imaging of scattered light; The multi-wavelength hybrid light source module includes a light source module, a beam mixing module, and a beam expansion module; wherein, The light source module is composed of a blue band coherent laser (1), a green band coherent laser (2), and a red band coherent laser (3), and is used to provide coherent light within the three bands of blue, green, and red; The beam mixing module is used to achieve uniform mixing of the coherent light of the blue, green and red bands of the light source module to obtain mixed parallel light; The beam expansion module is used to achieve amplification and collimation of mixed parallel light; The wavefront shaping and imaging module includes a uniform beam module, a sample to be imaged, a scattering medium, a 4F module, a light field control module, an image receiving module, and a data integration processing module; wherein, The uniform beam module is used to convert the collimated input mixed parallel light beam into an output beam with uniform intensity; The scattering medium is used to distort the mixed parallel light wavefront to obtain scattered light; The 4F module is used for variable magnification imaging to the light field control module and the image receiving module; The light field control module is used to perform pure amplitude wavefront shaping on the scattered light whose wavefront is distorted after passing through the scattering medium, thereby restoring the wavefront; The image receiving module is used to receive image information of three channels, R, G, and B respectively; The data integration processing module is used to use an intelligent optimization algorithm based on binary amplitude modulation to perform wavefront shaping on the scattered light, and to perform data processing on the collected image information and use an algorithm to fuse the image information of the three channels to obtain a color image.

Images