An automated microfluidic chip liquid injection device

Abstract

The present application relates to the technical fields of microfluidic chip, and particularly relates to an automatic microfluidic chip liquid injection device, which comprises a liquid injection mechanism, a moving mechanism and a rotary driving mechanism; the liquid injection mechanism comprises a liquid injection pipe, a liquid injection sleeve, an elastic sealing sleeve and an upper liquid injection sleeve pipe; the liquid injection pipe penetrates into the liquid injection sleeve, the elastic sealing sleeve is clamped between the liquid injection pipe and the liquid injection sleeve, and the elastic sealing sleeve is arranged at the liquid injection insertion end of the liquid injection pipe; the liquid injection sleeve is rotationally connected with the upper liquid injection sleeve pipe, and the rotation of the upper liquid injection sleeve pipe is used to move the liquid injection sleeve away from the upper liquid injection sleeve pipe; the rotary driving mechanism is installed on the liquid injection mechanism, and the rotary driving mechanism is used to drive the rotation of the upper liquid injection sleeve pipe; the liquid injection mechanism is installed on the moving mechanism, and the moving mechanism is used to drive the movement of the liquid injection mechanism, so that the liquid injection pipe moves into or out of the liquid injection port of the microfluidic chip, and the elastic sealing sleeve is compressed between the liquid injection port and the liquid injection sleeve.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microfluidic chip, and particularly relates to an automatic microfluidic chip liquid injection device. BACKGROUND

[0002] Microfluidic chip technology is to integrate sample preparation, reaction, separation, detection and other basic operation units of chemical, biological and medical analysis process on a micron-scale chip to automatically complete the whole analysis process. The technology has been widely researched and applied in the fields of biology, chemistry, medicine and the like.

[0003] At present, microfluidic chips are very common in microfluidic laboratories, but there is still a technical pain point of the microfluidic chip, that is, the liquid leakage prevention problem of the connection between the chip and the liquid inlet pipe. Since the connection pipe and the liquid inlet cannot be well matched, and the liquid pressure of the microfluidic chip pipe is high when injecting the cell sample liquid, the liquid inlet usually leaks.

[0004] Therefore, it has practical value to research an automatic microfluidic chip liquid injection device that can prevent liquid leakage during liquid injection. SUMMARY

[0005] The present application aims to provide an automatic microfluidic chip liquid injection device to solve the problem of easy liquid leakage in the prior art during liquid injection.

[0006] In order to solve the above technical problems, the present application provides an automatic microfluidic chip liquid injection device, comprising

[0007] a liquid injection mechanism, a moving mechanism and a rotating drive mechanism;

[0008] The liquid injection mechanism comprises a liquid injection pipe, a liquid injection sleeve, an elastic sealing sleeve and an upper liquid injection sleeve pipe;

[0009] The liquid injection pipe penetrates into the liquid injection sleeve, the elastic sealing sleeve is clamped between the liquid injection pipe and the liquid injection sleeve, and the elastic sealing sleeve is arranged at the liquid injection insertion end of the liquid injection pipe;

[0010] The liquid injection sleeve is rotationally connected with the upper liquid injection sleeve pipe, and the rotation of the upper liquid injection sleeve pipe is used to move the liquid injection sleeve away from the upper liquid injection sleeve pipe;

[0011] The rotating drive mechanism is installed on the liquid injection mechanism, and the rotating drive mechanism is used to drive the rotation of the upper liquid injection sleeve pipe;

[0012] The moving mechanism is installed on the liquid injection mechanism, and the moving mechanism is used to drive the movement of the liquid injection mechanism, so that the liquid injection pipe moves into or out of the liquid injection port of the microfluidic chip, and the elastic sealing sleeve is compressed between the liquid injection port and the liquid injection sleeve.

[0013] In one of the embodiments, the liquid injection sleeve is provided with a first constant diameter pipe section; the liquid injection pipe is provided with a liquid injection variable diameter section, which penetrates into the first constant diameter pipe section, and gradually decreases in diameter in the direction adjacent to the liquid injection port, and the maximum diameter of the liquid injection variable diameter section is greater than the diameter of the first constant diameter pipe section.

[0014] In one of the embodiments, the liquid injection sleeve is further provided with a second constant diameter pipe section and a variable width pipe section; the variable width pipe section and the second constant diameter pipe section are sequentially connected in the direction adjacent to the liquid injection port, and the diameter of the variable width pipe section gradually increases; the elastic sealing sleeve is arranged in the second constant diameter pipe section and / or the variable width pipe section, and the sleeve diameter of the elastic sealing sleeve is greater than the minimum diameter of the variable width pipe section.

[0015] In one of the embodiments, the elastic sealing sleeve comprises a thick diameter wall section, a variable diameter wall section and a narrow diameter wall section; the thick diameter wall section, the variable diameter wall section and the narrow diameter wall section are sequentially connected in the direction adjacent to the liquid injection port; the outer wall of the thick diameter wall section elastically abuts against the inner wall of the liquid injection sleeve; the inner wall of the thick diameter wall section, the inner wall of the variable diameter wall section and the inner wall of the narrow diameter wall section all elastically abut against the outer wall of the liquid injection pipe.

[0016] In one of the embodiments, the liquid injection mechanism further comprises a liquid injection limiting member sleeve and a limiting member; the liquid injection pipe is screwed into the liquid injection limiting member sleeve, and the liquid injection limiting member sleeve is slidably installed in the liquid injection sleeve; the limiting member abuts against the liquid injection limiting member sleeve, and the limiting member is arranged at the end of the liquid injection limiting member sleeve away from the liquid injection port, and the limiting member is used to make the liquid injection limiting member sleeve keep the tendency of moving towards the liquid injection port.

[0017] In one of the embodiments, the limiting member comprises a limiting rod and a limiting block; the limiting rod comprises a fixed end and an extended end, the fixed end is fixedly installed in the liquid injection sleeve, and the extended end is rotationally connected with the limiting block; the limiting block comprises a narrow end and a wide end, the narrow end abuts against the liquid injection limiting member sleeve, and the wide end abuts against the inner wall of the liquid injection sleeve, and the distance between the narrow end and the rotation point of the limiting block is greater than the distance between the wide end and the rotation point of the limiting block.

[0018] In one of the embodiments, the automatic micro-fluidic chip liquid injection device further comprises a chip clamping mechanism; the chip clamping mechanism comprises a leveling assembly, a placement table and a plurality of chip clamping pieces; the placement table is installed on the leveling assembly, and the leveling assembly is used to level the placement table; the plurality of chip clamping pieces are arranged opposite along the same axis, and each of the chip clamping pieces comprises a clamping part and a driving part; the clamping part is arranged on the placement table, the clamping part is in transmission connection with the driving part, and the driving part is used to drive the clamping part to move away from or close to the micro-fluidic chip to clamp the micro-fluidic chip.

[0019] In one of the embodiments, the automatic micro-fluidic chip liquid injection device further comprises a trigger mechanism; the trigger mechanism comprises a trigger spring and a trigger locking piece; the upper liquid injection sleeve is coaxially arranged with the liquid injection sleeve, a compression protruding wall is arranged on the outer wall of the upper liquid injection sleeve, and the compression protruding wall is arranged outside the liquid injection sleeve; the trigger spring is compressed between the compression protruding wall and the liquid injection sleeve; the trigger locking piece is arranged between the upper liquid injection sleeve and the liquid injection sleeve; the upper liquid injection sleeve is rotated to make the trigger locking piece in an unlocked state or a locked state; when the trigger locking piece is in the unlocked state, the trigger spring is used to push the liquid injection sleeve to move away from the upper liquid injection sleeve; when the trigger locking piece is in the locked state, the liquid injection sleeve is clamped in the upper liquid injection sleeve.

[0020] In one of the embodiments, the trigger locking piece comprises a trigger block, a locking channel and a trigger channel; the trigger block is arranged on the outer wall of the upper liquid injection sleeve; the locking channel and the trigger channel are arranged on the inner wall of the liquid injection sleeve, and the locking channel and the trigger channel are in communication with each other; the locking channel is arranged circumferentially around the outer wall of the upper liquid injection sleeve, and the axis direction of the trigger channel is the same as the axis direction of the upper liquid injection sleeve; the trigger block is slidably installed in the locking channel or the trigger channel.

[0021] In one of the embodiments, the automatic micro-fluidic chip liquid injection device further comprises a reset mechanism; a plurality of reset grooves are arranged on the outer wall of the liquid injection sleeve, and the plurality of reset grooves are arranged circumferentially around the outer wall of the liquid injection sleeve; the reset mechanism comprises a reset gear, a reset frame and a reset motor; the reset frame is provided with a through hole, and the liquid injection mechanism is installed in the through hole; the reset gear is arranged on the reset frame, the gear axis of the reset gear is perpendicular to the axis of the liquid injection sleeve, the reset gear is in meshing connection with the reset grooves, and the reset gear is in transmission connection with the reset motor.

[0022] In one of the embodiments, the liquid injection mechanism further comprises a buffer reset member; an inner wall of the liquid injection sleeve is provided with a spring pressing block; the buffer reset member comprises a buffer reset spring and a buffer reset sleeve; the buffer reset sleeve is axially movably arranged in the liquid injection sleeve; the reset spring is compressed between an end of the buffer reset sleeve away from the liquid injection port and the spring pressing block; and the other end of the buffer reset sleeve can move out of or into the liquid injection sleeve.

[0023] The more prominent beneficial effects of the present application are as follows:

[0024] I. Since the liquid injection tube penetrates into the liquid injection sleeve, the liquid injection tube and the liquid injection sleeve elastically clamp the elastic sealing sleeve, the elastic sealing sleeve is arranged at the liquid injection insertion end of the liquid injection tube; the liquid injection sleeve is rotationally connected with the upper liquid injection sleeve tube, and the rotation of the upper liquid injection sleeve tube is used to move the liquid injection sleeve away from the upper liquid injection sleeve tube, so that when the liquid injection tube is inserted into the liquid injection port to a greater depth, the liquid injection sleeve will gradually abut against the liquid injection port, and when the liquid injection sleeve continuously maintains the trend of moving towards the liquid injection port, the liquid injection sleeve is pressed against the liquid injection port, and the gap between the liquid injection sleeve and the liquid injection port is continuously sealed and closed by the elastic sealing sleeve, so that the sealing during the liquid injection process is realized, and liquid cannot flow out of the liquid injection port or the liquid injection tube.

[0025] II. Since the liquid injection tube is provided with a liquid injection variable diameter section, and the liquid injection sleeve is provided with a first constant diameter tube section; the liquid injection variable diameter section of the liquid injection tube penetrates into the first constant diameter tube section, and the tube diameter of the liquid injection variable diameter section gradually decreases in the direction adjacent to the liquid injection port, the maximum tube diameter of the liquid injection variable diameter section is greater than the tube diameter of the first constant diameter tube section, and the liquid injection tube is fixed in the liquid injection sleeve, so that the gradually decreasing tube diameter of the liquid injection tube is abutted and pressed in the constant diameter tube section, thereby maintaining the sealed connection between the liquid injection tube and the liquid injection sleeve.

[0026] III. Since the liquid injection tube is continuously subjected to downward force by the limiting member, the liquid injection tube can continuously abut against the elastic sealing sleeve, and the elastic sealing sleeve is elastically clamped by the liquid injection tube and the liquid injection sleeve, which on one hand avoids the liquid injection tube from being loosened from the elastic sealing sleeve during the liquid injection process, thereby causing the liquid injection of the liquid injection tube to flow out of the elastic upper part of the liquid injection sleeve to the outside; and on the other hand avoids the liquid injection tube from being inserted into the liquid injection port to a depth that is too deep, thereby causing the broken condition. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort based on these drawings.

[0028] Figure 1 is the overall structure schematic diagram provided by the first embodiment of the present application;

[0029] Figure 2 is an exploded structural schematic view of the liquid injection mechanism provided by Embodiment One of the present application;

[0030] Figure 3 is a cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment One of the present application;

[0031] Figure 4 is a partial cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment One of the present application;

[0032] Figure 5 is a structural schematic view of the chip clamping mechanism provided by Embodiment One of the present application;

[0033] Figure 6 is an exploded structural schematic view of the chip clamping mechanism provided by Embodiment One of the present application;

[0034] Figure 7 is a partial structural schematic view of the trigger mechanism provided by Embodiment One of the present application;

[0035] Figure 8 is a partial structural schematic view of the liquid inlet mechanism provided by Embodiment One of the present application;

[0036] Figure 9 is a structural schematic view of the reagent tube provided by Embodiment One of the present application;

[0037] Figure 10 is a cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment Two of the present application;

[0038] Figure 11 is a cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment Three of the present application Figure 1 ;

[0039] Figure 12 is a cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment Three of the present application Figure 2 ;

[0040] Figure 13 is a partial cross-sectional structural schematic view of the liquid injection mechanism provided by Embodiment Three of the present application;

[0041] Figure 14 is a cross-sectional structural schematic view of the liquid injection mechanism and microfluidic chip provided by Embodiment Four of the present application.

[0042] The reference signs are as follows:

[0043] 1, liquid injection mechanism; 10, liquid injection pipe; 100, liquid injection variable diameter section; 101, liquid injection narrowing section; 102, liquid injection constant diameter section; 11, liquid injection sleeve; 110, middle liquid injection sleeve; 1100, reset groove; 111, lower liquid injection sleeve; 1110, first constant diameter pipe section; 1111, second constant diameter pipe section; 1112, third constant diameter pipe section; 1113, variable wide diameter pipe section; 1114, variable narrow diameter pipe section; 1115, spring pressing block; 12, elastic sealing sleeve; 120, thick diameter wall section; 121, variable diameter wall section; 122, narrow diameter wall section; 13, upper liquid injection sleeve; 130, compression convex wall; 14, liquid injection limiting member sleeve; 15, limiting member; 150, limiting rod; 151, limiting block; 16, buffer reset member; 160, buffer reset spring; 161, buffer reset sleeve; 1610, buffer reset rod; 1611, buffer reset block; 17, clamping assembly block;

[0044] 2, moving mechanism; 20, vertical moving unit; 21, horizontal moving unit; 22, longitudinal moving unit;

[0045] 3, rotating drive mechanism; 30, rotating fixed frame; 31, frameless motor stator; 32, frameless motor rotor;

[0046] 4, chip clamping mechanism; 40, leveling assembly; 400, leveling frame; 401, leveling probe; 402, leveling member; 4020, leveling motor; 4021, leveling screw; 4022, leveling spring; 4023, leveling nut; 41, placement table; 42, chip clamping member; 420, clamping part; 421, driving part;

[0047] 5, trigger mechanism; 50, trigger spring; 51, trigger locking member; 510, trigger block; 511, locking channel; 512, trigger channel;

[0048] 6, reset mechanism; 60, reset gear; 61, reset frame; 62, reset motor;

[0049] 7, liquid inlet mechanism; 70, air pressure member; 700, liquid injection pipe air nozzle; 71, cover; 72, reagent fixing member; 720, reagent pipe; 7200, rubber one-way membrane; 7201, reagent pipe plug; 7202, reagent pipe cover;

[0050] 8, microfluidic chip; 80, liquid injection port. DETAILED DESCRIPTION

[0051] The technical solutions in the embodiments of the application will be described clearly and completely below with reference to the drawings in the embodiments of the application.

[0052] Microfluidic chip is very common in microfluidic laboratory, but there is still a technical pain point of the microfluidic chip, which is the leakage prevention problem of the connection between the chip and the liquid inlet pipe. Due to the poor matching of the connection pipe and the liquid inlet, and the high hydraulic pressure of the microfluidic chip pipe when injecting the cell sample liquid, the liquid inlet usually leaks.

[0053] In order to solve the above problems, the present application provides an automatic microfluidic chip liquid injection device, the core of which is to drive the liquid injection sleeve to move towards the liquid injection port by rotating the liquid injection sleeve and the upper liquid injection sleeve pipe, so that the elastic sealing sleeve is compressed between the liquid injection port and the liquid injection sleeve. The specific implementation is as follows.

[0054] Embodiment one

[0055] The first embodiment of the present application provides an automatic microfluidic chip liquid injection device, please refer to Figures 1 to 9 , including liquid injection mechanism 1, moving mechanism 2 and rotating drive mechanism 3; the liquid injection mechanism 1 includes liquid injection pipe 10, liquid injection sleeve 11, elastic sealing sleeve 12 and upper liquid injection sleeve pipe 13; the liquid injection pipe 10 penetrates into the liquid injection sleeve 11, the elastic sealing sleeve 12 is clamped between the liquid injection pipe 10 and the liquid injection sleeve 11, and the elastic sealing sleeve 12 is arranged at the liquid injection insertion end of the liquid injection pipe 10; the liquid injection sleeve 11 is rotatably connected with the upper liquid injection sleeve pipe 13, and the rotation of the upper liquid injection sleeve pipe 13 is used to move the liquid injection sleeve 11 away from the upper liquid injection sleeve pipe 13; the rotating drive mechanism 3 is installed on the liquid injection mechanism 1, and the rotating drive mechanism 3 is used to drive the upper liquid injection sleeve pipe 13 to rotate; the moving mechanism 2 is installed on the liquid injection mechanism 1, and the moving mechanism 2 is used to drive the liquid injection mechanism 1 to move, so that the liquid injection pipe 10 moves into or out of the liquid injection port 80 of the microfluidic chip 8, and the elastic sealing sleeve 12 is compressed between the liquid injection port 80 and the liquid injection sleeve 11. In application, the liquid injection pipe 10 penetrates into the liquid injection sleeve 11 and is installed therein, then the moving mechanism 2 drives the liquid injection mechanism 1 to move to the position corresponding to the liquid injection port 80, and the liquid injection pipe 10 is inserted into the liquid injection port 80, then the rotating drive mechanism 3 drives the upper liquid injection sleeve pipe 13 to rotate, drives the liquid injection sleeve 11 and the liquid injection pipe 10 in the liquid injection sleeve 11 to continue to move towards the liquid injection port 80, when the liquid injection sleeve 11 abuts against the liquid injection port 80 or the microfluidic chip, the elastic sealing sleeve 12 is compressed by the liquid injection sleeve 11 and the liquid injection port 80, the elastic sealing sleeve 12 realizes interference sealing cooperation with the liquid injection port 80 and the liquid injection sleeve 11, so as to realize the sealing purpose.

[0056] In this embodiment, the moving mechanism 2 related to the above is as follows Figure 1As shown, the moving mechanism 2 includes a vertical moving unit 20, a lateral moving unit 21 and a longitudinal moving unit 22, the lateral moving unit 21 is slidingly installed on the vertical moving unit 20, the liquid injection mechanism 1 and the rotating driving mechanism 3 are installed on the lateral moving unit 21, the microfluidic chip is installed on the longitudinal moving unit 22, the vertical moving unit 20 and the lateral moving unit 21 are used to move the liquid injection mechanism 1 and the rotating driving mechanism 3 in the vertical and lateral directions on the same plane, and the longitudinal moving unit 22 is used to longitudinally move the microfluidic chip to the corresponding plane, so that the liquid injection mechanism 1 can be aligned with and moved into or out of the liquid injection port 80 of the microfluidic chip.

[0057] In the embodiment, the vertical moving unit 20, the lateral moving unit 21 and the longitudinal moving unit 22 are all linear moving units, and the linear moving units can be selected in various ways, including but not limited to a screw block moving unit, a gear and rack moving unit and a cylinder and piston unit, and a person skilled in the art can select according to actual needs.

[0058] In the embodiment, the rotating driving mechanism 3 is as shown in the accompanying drawings. Figure 2 As shown, the rotating driving mechanism 3 includes a rotating fixed frame 30, a frameless motor stator 31 and a frameless motor rotor 32, the frameless motor stator 31 is installed on the rotating fixed frame 30, the frameless motor rotor 32 is installed in the frameless motor stator 31, and the upper liquid injection sleeve 13 of the liquid injection mechanism 1 is installed in the frameless motor rotor 32. When applied, the permanent magnet on the frameless motor rotor 32 generates a magnetic field, and the coil on the stator generates another magnetic field when the current passes through. When the two magnetic fields interact, according to the law of Lorentz force, a torque is generated on the rotor, so that the frameless motor rotor 32 and the upper liquid injection sleeve 13 rotate.

[0059] In the embodiment, the rotating fixed frame 30 is provided with a through hole for liquid inlet, and the through hole is in communication with the upper liquid injection sleeve 13 of the liquid injection mechanism 1.

[0060] In the embodiment, the liquid injection mechanism 1 is as shown in the accompanying drawings. Figure 2 and Figure 3As shown, the axis of the liquid injection mechanism 1 is vertically arranged, that is, the axis of the liquid injection mechanism 1 is perpendicular to the horizontal plane, the liquid injection mechanism 1 comprises a liquid injection pipe 10, a liquid injection sleeve 11, an elastic sealing sleeve 12 and an upper liquid injection sleeve pipe 13, the liquid injection pipe 10 is detachably arranged in the liquid injection sleeve 11, the liquid injection pipe 10 and the liquid injection sleeve 11 are adjacent to one end of the liquid injection port 80, and the elastic sealing sleeve 12 is elastically clamped therebetween, the upper liquid injection sleeve pipe 13 is rotatably arranged in the liquid injection sleeve 11 away from the liquid injection port 80, the upper liquid injection sleeve pipe 13 is rotatably connected with the rotary driving mechanism 3, in application, the upper liquid injection sleeve pipe 13 is driven to rotate by the rotary driving mechanism 3, the upper liquid injection sleeve pipe 13 drives the liquid injection sleeve 11 to rotate, so that the liquid injection sleeve 11 moves away from the upper liquid injection sleeve pipe 13 (that is, moves towards the liquid injection port 80), when the liquid injection sleeve 11 moves to the liquid injection port 80 and abuts against it, the elastic sealing sleeve 12 between the liquid injection port 80 and the liquid injection sleeve 11 is gradually compressed, the compression degree of the elastic sealing sleeve 12 increases with the depth of the movement of the liquid injection sleeve 11, so that the elastic sealing sleeve 12 is in interference sealing fit with the liquid injection port 80 and the liquid injection sleeve 11, when the liquid to be injected is input into the upper liquid injection sleeve pipe 13, the liquid to be injected flows into the liquid injection pipe 10, the liquid injection pipe 10, the liquid injection sleeve 11 and the liquid injection port 80 are in interference sealing fit with the elastic sealing sleeve 12 to achieve effective sealing.

[0061] In this embodiment, as shown in Figure 2 , Figure 3 and Figure 7 , in order to realize the rotary connection of the liquid injection sleeve 11 and the upper liquid injection sleeve pipe 13, a trigger mechanism 5 is further included, the trigger mechanism 5 comprises a trigger spring 50 and a trigger locking piece 51; the upper liquid injection sleeve pipe 13 is coaxially arranged with the liquid injection sleeve 11, a compression protruding wall 130 is arranged on the outer wall of the upper liquid injection sleeve pipe 13 and outside the liquid injection sleeve 11; the trigger spring 50 is compressed between the compression protruding wall 130 and the liquid injection sleeve 11; the trigger locking piece 51 is arranged between the upper liquid injection sleeve pipe 13 and the liquid injection sleeve 11; the upper liquid injection sleeve pipe 13 rotates to make the trigger locking piece 51 in an unlocked state or a locked state; when the trigger locking piece 51 is in the unlocked state, the trigger spring 50 is used to push the liquid injection sleeve 11 to move away from the upper liquid injection sleeve pipe 13; when the trigger locking piece 51 is in the locked state, the liquid injection sleeve 11 is clamped in the upper liquid injection sleeve pipe 13, in application, when it is needed to move the liquid injection sleeve 11 downward, the rotary driving mechanism 3 drives the upper liquid injection sleeve pipe 13 to rotate until the trigger locking piece 51 is in the unlocked state, in the unlocked state, the compression energy of the trigger spring 50 is released, and the compression energy of the trigger spring 50 is converted into energy to drive the liquid injection sleeve 11 to move away from the upper liquid injection sleeve pipe 13, so that the liquid injection sleeve 11 moves away from the upper liquid injection sleeve pipe 13.

[0062] In the embodiment, for the trigger lock 51, the trigger lock 51 comprises a trigger block 510, a locking channel 511 and a trigger channel 512, as shown in Figure 2 and Figure 7 The trigger block 510 is arranged on the outer wall of the upper liquid injection sleeve 13; the locking channel 511 and the trigger channel 512 are arranged on the inner wall of the liquid injection sleeve 11, and the locking channel 511 and the trigger channel 512 are in communication with each other; the locking channel 511 is arranged in the circumferential direction of the outer wall of the upper liquid injection sleeve 13, and the axis direction of the trigger channel 512 is the same as the axis direction of the upper liquid injection sleeve 13; the trigger block 510 is slidably installed in the locking channel 511 or the trigger channel 512; in use, the rotation driving mechanism 3 drives the rotation of the upper liquid injection sleeve 13, thereby driving the rotation of the trigger block 510 in the locking channel 511; only when the trigger block 510 is rotated to the trigger channel 512, the trigger mechanism 5 is in the unlocked state, and the liquid injection sleeve 11 can move axially.

[0063] After the trigger mechanism 5 is used, at least the following advantages are obtained: first, the entire liquid injection mechanism 1 moves to the vicinity of the liquid injection port 80; when the moving displacement of the liquid injection sleeve 11 is less than the compression amount of the trigger spring 50 can make the liquid injection sleeve 11 keep moving away from the upper liquid injection sleeve 13, that is, the liquid injection sleeve 11 keeps being compressed with the liquid injection port 80, so that the elastic sealing sleeve 12 is continuously compressed by the liquid injection sleeve 11 and the liquid injection port 80, thereby achieving better interference sealing connection; second, due to the arrangement of the trigger mechanism 5, compared with the ordinary threaded connection or other rotation mode, the axial movement of the liquid injection sleeve 11 is strictly controlled; only when the trigger mechanism 5 is in the unlocked state, the liquid injection sleeve 11 can move, thereby effectively improving the stability of operation.

[0064] In the embodiment, as shown in Figure 4 In order to realize the assembly connection of the liquid injection pipe 10 in the liquid injection sleeve 11, the liquid injection pipe 10 and the liquid injection sleeve 11 are connected through threads, that is, the liquid injection pipe 10 is provided with external threads, the liquid injection sleeve 11 is provided with internal threads, and the liquid injection pipe 10 is connected with the liquid injection sleeve 11 through the external threads and the internal threads, thereby realizing the fixation of the liquid injection pipe 10 in the liquid injection sleeve 11.

[0065] In the embodiment, as shown in Figure 4As shown, in order to enhance the sealing assembly effect of the liquid injection pipe 10 in the liquid injection sleeve 11, the liquid injection sleeve 11 is provided with a first constant diameter pipe segment 1110; the liquid injection pipe 10 is provided with a liquid injection variable diameter segment 100, the liquid injection variable diameter segment 100 penetrates into the first constant diameter pipe segment 1110, and the pipe diameter of the liquid injection variable diameter segment 100 gradually decreases in the direction adjacent to the liquid injection port 80; the maximum pipe diameter of the liquid injection variable diameter segment 100 is greater than the pipe diameter of the first constant diameter pipe segment 1110; in application, after one end of the liquid injection pipe 10 is screwed into the liquid injection sleeve 11, the other end of the liquid injection pipe 10 will be abutted and pressed in the first constant diameter pipe segment 1110 of the liquid injection sleeve 11 due to the existence of the liquid injection variable diameter segment 100; after this kind of arrangement is adopted, on the one hand, the sealed connection between the liquid injection pipe 10 and the liquid injection sleeve 11 is ensured, the liquid injection pipe 10 is prevented from sliding out of the liquid injection sleeve 11 or the elastic sealing sleeve 12, and the sealed state of the liquid injection pipe 10 is effectively maintained; on the other hand, the depth of the liquid injection pipe 10 inserted into the microfluidic chip can be prevented from being too deep, and the liquid injection pipe 10 can be prevented from being broken.

[0066] In the embodiment, as shown in Figure 4 In order to enhance the sealing effect of the liquid injection sleeve 11 and the elastic sealing sleeve 12, the liquid injection sleeve 11 is further provided with a second constant diameter pipe segment 1111 and a variable width pipe segment 1113; the variable width pipe segment 1113 and the second constant diameter pipe segment 1111 are sequentially connected in the direction adjacent to the liquid injection port 80, and the pipe diameter of the variable width pipe segment 1113 gradually increases; the elastic sealing sleeve 12 is arranged in the second constant diameter pipe segment 1111 and / or the variable width pipe segment 1113, and the sleeve diameter of the elastic sealing sleeve 12 is greater than the minimum pipe diameter of the variable width pipe segment 1113; in application, when the elastic sealing sleeve 12 is extruded by the liquid injection port 80, the elastic deformation of the elastic sealing sleeve 12 can be supported by the inclined wall of the variable width pipe, so that the interference fit effect of the elastic sealing sleeve 12 and the liquid injection sleeve 11 is better.

[0067] In the embodiment, for the liquid injection sleeve 11, as shown in Figure 4 The liquid injection sleeve 11 includes a middle liquid injection sleeve pipe 110 and a lower liquid injection sleeve pipe 111; one end of the middle liquid injection sleeve pipe 110 is rotationally connected with the upper liquid injection sleeve pipe 13 in the direction adjacent to the liquid injection port 80; the other end of the middle liquid injection sleeve pipe 110 is detachably connected with the lower liquid injection sleeve pipe 111; the lower liquid injection sleeve pipe 111 includes the first constant diameter pipe segment 1110, the second constant diameter pipe segment 1111, the third constant diameter pipe segment 1112, the variable width pipe segment 1113 and the variable narrow pipe segment 1114; the third constant diameter pipe segment 1112, the variable narrow pipe segment 1114, the first constant diameter pipe segment 1110, the variable width pipe segment 1113 and the second constant diameter pipe segment 1111 are sequentially connected in the direction adjacent to the liquid injection port 80.

[0068] In the embodiment, for the liquid injection pipe 10, as shown in Figure 4As shown, the liquid injection pipe 10 includes a liquid injection variable diameter section 100, a liquid injection narrowing section 101, and a liquid injection constant diameter section 102, and in the direction adjacent to the liquid injection port 80, the liquid injection constant diameter section 102, the liquid injection narrowing section 101, and the liquid injection variable diameter section 100 are sequentially connected.

[0069] The liquid injection narrowing section 101 of the liquid injection pipe 10 is connected with the variable diameter pipe section 1114 of the lower liquid injection sleeve 11, and the liquid injection variable diameter section 100 of the liquid injection pipe 10 is connected with the first constant diameter pipe section 1110 of the liquid injection sleeve 11, and after adopting this arrangement, the sealing assembly effect of the liquid injection pipe 10 in the liquid injection sleeve 11 can be effectively achieved.

[0070] In this embodiment, the detachable connection mode of the middle liquid injection sleeve pipe 110 and the lower liquid injection sleeve pipe 111 is as shown in Figure 2 As shown, the middle liquid injection sleeve pipe 110 is externally provided with a first rotating groove, the lower liquid injection sleeve pipe 111 is externally provided with rotating blocks, and the rotating blocks are formed with a second rotating groove, the clamping assembly block 17 is rotatably sleeved on the outside of the middle liquid injection sleeve pipe 110, the clamping assembly block 17 is provided with a notch and a connecting portion, when the connecting block of the clamping assembly block 17 is rotated on the first rotating groove and the second rotating groove, the lower liquid injection sleeve pipe 111 is assembled on the middle liquid injection sleeve pipe 110 through the clamping assembly block 17, when the notch of the clamping assembly block 17 is rotated to the rotating block of the lower liquid injection sleeve pipe 111, the lower liquid injection sleeve pipe 111 can be detached from the middle liquid injection sleeve pipe 110, thereby realizing the detachable connection of the middle liquid injection sleeve pipe 110 and the lower liquid injection sleeve pipe 111, and in application, the lower liquid injection sleeve pipe 111 can be replaced in style to adapt to different micro-fluidic chips.

[0071] In this embodiment, in order to better seal and connect the liquid injection sleeve 11 with the liquid injection port 80, the liquid injection port 80 of the liquid injection sleeve 11 is provided with a liquid injection convex wall, and the liquid injection convex wall is externally provided with threads; the inner wall of the lower liquid injection sleeve pipe 111 of the liquid injection sleeve 11 is provided with threads, and the liquid injection sleeve 11 is threadedly connected with the liquid injection port 80, and in application, the rotation of the upper liquid injection sleeve pipe 13 can drive the liquid injection sleeve 11 to rotate, thereby driving the liquid injection sleeve 11 to be rotationally connected with the liquid injection port 80, so that the liquid injection sleeve 11 is connected with the liquid injection port 80 in a guided manner, and the elastic sealing sleeve 12 can better be interference sealed with the liquid injection port 80.

[0072] In this embodiment, as shown in Figure 3As shown, in order to enhance the interference sealing effect of the elastic sealing sleeve 12, the elastic sealing sleeve 12 includes a thick diameter wall section 120, a variable diameter wall section 121 and a narrow diameter wall section 122; along the direction adjacent to the injection port 80, the thick diameter wall section 120, the variable diameter wall section 121 and the narrow diameter wall section 122 are connected in sequence; the outer wall of the thick diameter wall section 120 elastically abuts against the inner wall of the injection sleeve 11; the inner wall of the thick diameter wall section 120, the inner wall of the variable diameter wall section 121 and the inner wall of the narrow diameter wall section 122 all elastically abut against the outer wall of the injection tube 10. When in use, the inner and outer sides of the elastic sealing sleeve 12 abut against the injection tube 10 and the injection sleeve 11 respectively. When the injection port 80 abuts against the elastic sealing sleeve 12, the thick diameter wall section 120 of the elastic sealing sleeve 12 can achieve a good interference fit effect.

[0073] After adopting the elastic sealing sleeve 12, there are at least the following advantages. First, when the liquid injection port 80 and the elastic sealing sleeve 12 are pressed tightly, the liquid injection port 80 and the elastic sealing sleeve 12 form an interference sealing connection, which can effectively prevent the liquid in the liquid injection port 80 from overflowing; second, when the liquid injection port 80 presses the elastic sealing sleeve 12, the elastic deformation of the elastic sealing sleeve 12 can be supported by the inclined wall of the widened diameter tube in the liquid injection sleeve 11, so that the elastic sealing sleeve 12 and the liquid injection port 80 form a better interference sealing fit; third, the elastic sealing sleeve 12 and the liquid injection tube 10 are elastically sealed to prevent overflow from the injection tube 10 and the elastic sealing sleeve 12.

[0074] In this embodiment, further, in order to realize the reset of the injection mechanism 1 after injection, as shown in FIG. Figure 2 As shown, it also includes a reset mechanism 6; a plurality of reset grooves 1100 are provided on the outer wall of the injection sleeve 11, and the plurality of reset grooves 1100 are arranged circumferentially around the outer wall of the injection sleeve 11; the reset mechanism 6 is engaged with the reset groove 1100, and the reset mechanism 6 is used to move the injection mechanism 1 away from the injection port 80, so that the injection mechanism 1 moves and resets.

[0075] In this embodiment, if Figure 2 As shown, the reset mechanism 6 includes a reset gear 60, a reset frame 61 and a reset motor 62; the reset frame 61 is provided with a through hole, and the injection mechanism 1 is installed in the through hole; the reset gear 60 is provided on the reset frame 61, and the reset frame 61 is fixedly connected to the rotating fixed frame 30, the gear axis of the reset gear 60 and the axis of the injection sleeve 11 are perpendicular to each other, the reset gear 60 is meshed with the reset groove 1100, and the reset gear 60 is transmission-connected to the reset motor 62. After adopting this arrangement, when the injection mechanism 1 completes the injection, the reset motor 62 drives the reset gear 60 to rotate, thereby driving the injection sleeve 11 of the injection mechanism 1 to move away from the injection port 80, thereby achieving reset, and the automation and reuse of the injection device can be realized.

[0076] In the embodiment, further, in order to realize clamping and fixing of the micro-fluidic chip, as shown in Figure 5 and Figure 6 , the chip clamping mechanism 4 is further included; the chip clamping mechanism 4 is installed on the longitudinal moving unit 22, and the chip clamping mechanism 4 includes the leveling assembly 40, the placing table 41 and a plurality of chip clamping pieces 42; the placing table 41 is installed on the leveling assembly 40, and the leveling assembly 40 is used for leveling the placing table 41; the plurality of chip clamping pieces 42 are arranged oppositely along the same axis, and the chip clamping piece 42 includes the clamping part 420 and the driving part 421; the clamping part 420 is arranged on the placing table 41, the clamping part 420 is in transmission connection with the driving part 421, and the driving part 421 is used for driving the clamping part 420 to move away from or close to the micro-fluidic chip to clamp the micro-fluidic chip; after the arrangement is adopted, the micro-fluidic chip is clamped on the chip clamping piece 42, and the micro-fluidic chip is leveled by the leveling assembly 40, so that the micro-fluidic chip can be kept horizontal during liquid injection, and a line-plane vertical relationship between the micro-fluidic chip and the liquid injection mechanism 1 is formed, that is, the axis of the liquid injection pipe 10 can be coaxially arranged with the axis of the liquid inlet, the elastic sealing sleeve 12 can be kept vertical with the axis of the liquid injection port 80 when the liquid injection sleeve 11 abuts against the liquid injection port 80, and the contact position is more uniform when the two are in interference sealing cooperation, thereby avoiding the problem that the sealing property is reduced due to poor contact between the elastic sealing sleeve 12 and the liquid injection sleeve 11.

[0077] In the embodiment, for the leveling assembly 40, as shown in Figure 5 and Figure 6 , the leveling assembly 40 includes the leveling frame 400, the leveling probe 401 and a plurality of leveling pieces 402, the leveling frame 400 is installed on the longitudinal moving unit 22, the leveling frame 400 is an X-shaped frame body, the leveling piece 402 is arranged at each corner of the leveling frame 400, the placing table 41 is installed on each leveling piece 402, and the leveling piece 402 is used for leveling, the leveling probe 401 abuts against the placing table 41, and the leveling probe 401 is used for detecting whether the placing table 41 is horizontal or not; after the arrangement is adopted, the leveling piece 402 can level the placing table 41, and when the leveling probe 401 detects that the placing table 41 is not leveled, the leveling piece 402 is continuously controlled to level until the placing table 41 is leveled.

[0078] In the embodiment, as shown in Figure 5 and Figure 6 , specifically, the leveling piece 402 includes the leveling motor 4020, the leveling screw 4021, the leveling spring 4022 and the leveling nut 4023, the leveling motor 4020 is installed on the leveling frame 400, the leveling screw 4021 is in transmission connection on the leveling motor 4020, the one end of the leveling screw 4021 away from the leveling motor 4020 is connected with the leveling nut 4023, and the leveling spring 4022 is compressed between the leveling nut 4023 and the leveling frame 400.

[0079] In the embodiment, asFigure 5 and Figure 6 As shown, the driving part 421 of the chip clamping part 42 is a micro lead screw and a lead screw slider, and the clamping part 420 of the chip clamping part 42 is a fixed claw, which is fixed on the lead screw slider. When in use, the fixed claw is driven by the micro lead screw and can be moved away from or closer to the microfluidic chip, thereby achieving the purpose of fixed clamping.

[0080] In this embodiment, further, in order to achieve the clamping and fixing of the microfluidic chip, as shown in FIG. Figure 8 As shown, it also includes a liquid inlet mechanism 7; the liquid inlet mechanism 7 is connected to the upper liquid injection sleeve 11, and the liquid inlet mechanism 7 is used to detect the sealing of the device and to fill the liquid, such as Figure 1 As shown, the liquid inlet mechanism 7 includes a pneumatic component 70, a sealing cover 71, a reagent fixing component 72, an injection converter housing, a pressure cover, a connector rubber ring and a converter connector. The top of the sealing cover is equipped with a pneumatic component, and the bottom of the sealing cover is equipped with a rotatable reagent fixing component. The reagent fixing component is used to install the reagent tube. The sealing cover and the injection converter housing form a sealed space. The reagent fixing component is arranged in the sealed space. The bottom of the injection converter housing is provided with an injection connecting hole, and the connecting hole is equipped with a connector rubber ring and a converter connector. After adopting this setting method, the pneumatic component can be used to detect air tightness, and the reagent fixing component can be used to fix the reagent tube.

[0081] In this embodiment, for the pneumatic member 70, Figure 8 As shown, the pneumatic component 70 includes a connecting nut, a cylinder frame, a micro cylinder sleeve, a liquid injection tube nozzle 700 and a cylinder rod. The cylinder frame can be rotatably mounted on the pressure cover, and the liquid injection tube nozzle 700 is installed in the center of the cylinder frame. One end of the liquid injection tube nozzle 700 can be connected to the pneumatic mechanism through a connecting nut, and the other end of the liquid injection tube nozzle is connected to the sealed space; the micro cylinder sleeve and the cylinder rod are fixed on the peripheral side of the cylinder frame, and the micro cylinder sleeve, the liquid injection tube nozzle 700 and the cylinder rod are used to push the reagent tube cover to compress the reagent tube.

[0082] In this embodiment, for the reagent fixing member 72, as shown in FIG. Figure 8 and Figure 9 As shown, the reagent fixing part 72 includes a converter spring, a reagent tube cover, a reagent tube 720, and a reagent tube rack. The test tube rack can be rotatably installed in the liquid injection converter housing, and the reagent tube and the reagent tube cover are installed on the peripheral side of the test tube rack. The rotation track of the reagent tube covers the cylinder rod and the liquid injection tube nozzle. During application, after the reagent tube is filled with reagent liquid, the reagent tube rotates and aligns with the liquid injection tube nozzle. When liquid injection is required, the liquid injection tube nozzle is connected to the pneumatic mechanism, and the pneumatic mechanism pumps air to make the reagent in the reagent tube flow into the liquid injection connecting hole, and makes the liquid pass through the upper liquid injection sleeve 13, the liquid injection sleeve 11 and the liquid injection tube 10 in turn, and finally injected into the liquid injection port 80 of the microfluidic chip.

[0083] It should be pointed out that if Figure 9 As shown, a sealing rubber one-way membrane 7200 is provided at the upper end of the reagent tube to block the air circulation between the outside and the inside of the tube. The lower end of the reagent tube gradually shrinks. When adding solution to the reagent tube, first plug the lower end of the reagent tube with a reagent tube plug 7201, and tighten the reagent tube cover 7202 after filling. When in use, pull out the reagent tube plug. At this time, because the rubber membrane blocks the air circulation, the reagent in the tube will not flow out.

[0084] As can be seen from the above, the basic structure and principle of this solution will be explained below in conjunction with the specific usage method.

[0085] The first step is the microfluidic chip loading and fixing step, such as Figure 1 As shown, the microfluidic chip is first placed on the placement table 41. The platform pressure sensing device automatically recognizes that the chip has been placed on the platform and preliminarily determines whether the position of the microfluidic chip is placed in the specified appropriate area. There is a rubber anti-slip pad on the bottom of the fixture, and the rubber pad is drawn with a guide placement area line; after confirming that the chip is placed correctly, the micro screw starts to start, driving the fixed claws to move toward the chip to clamp the microfluidic chip. During the contact process, a preliminary contact is first made to test whether the chip can be grasped by the four fixed claws. After the preliminary detection is completed, the movement continues. When the pressure sensor in the fixed claw detects a certain pressure, it stops moving. If the pressure signals fed back by the pressure sensors of the four fixed claws are significantly different, the device automatically determines that the microfluidic chip is fixed in an incorrect manner, and the micro screw device is loosened and a warning is issued on the display screen on the base control platform until the operator readjusts the chip position. There are three modes for chip clamping, namely loose clamping mode, normal clamping mode and over-tight clamping mode. The operator adjusts the clamping mode according to the manufacturing material of the microfluidic chip and actual needs. If there is no adjustment, the system defaults to normal clamping mode.

[0086] The second step is the automatic leveling step, such as Figure 1As shown, after the microfluidic chip is placed on the placement table 41 and fixed, the XYZ axis screw device composed of the horizontal moving unit 21, the vertical moving unit 20 and the longitudinal moving unit 22 drives the slider to move to the limiting position before automatic leveling starts, and the correction and homing of the coordinates of the liquid injection mechanism 1 are performed; after the homing coordinates are cleared, the YZ axis screw moving device composed of the horizontal moving unit 21 and the vertical moving unit 20 drives the liquid injection mechanism 1 to move to the liquid injection area, and the longitudinal moving unit 22 drives the placement table 41 to move to the liquid injection area and is fixed and no longer moves, the high-definition camera on the valve body and the visual recognition technology detect the chip position, after the detection is completed, the YZ axis screw device is started, the system is adjusted to the leveling mode, the screw moves up and down to drive the liquid injection mechanism 1 to move, the liquid injection sleeve 11 contacts the four corner points of the chip, the height of the four corner points of the chip in the Z direction is measured, when the measured data exceeds the specified range, the system automatically starts four leveling reduction motors, the motor drives the leveling screw 4021 to rotate and engage with the leveling nut 4023 to realize the small movement up and down, and the leveling spring 4022 is pressed to prevent the loosening between the screw and the nut, after the motor leveling is completed, the liquid injection mechanism 1 will be tested again whether it has been leveled by four-point positioning and distance measurement, the screw device is homed to the limit, and enters the solution loading mode.

[0087] The third step is the reagent tube loading step, as shown in Figure 1 When the leveling is completed, the moving mechanism 2 is homed, and the system automatically reminds the operator that the liquid injection tube 10 can be placed according to the prompt, the display screen of the base control platform prompts the number of reagent tubes that should be placed at present, the number is set by the operator in the computer software, after the screen display is placed, the operator clicks complete on the operation screen, the reagent tube rack automatically rotates to the next numbered tube rack, up to three reagent tubes can be placed, after the placement is completed, the sensor on the tube rack detects the number of tube racks placed and whether it is correctly placed, if there is deviation, a warning will be issued to reposition, after the placement is completed, the reagent tube rack rotation adjustment is required first to reach the liquid injection position of the reagent tube.

[0088] The fourth step is the liquid injection mechanism 1 and the liquid injection port 80 docking step, as shown in Figure 1As shown, the high-definition camera automatically identifies the position of the microfluidic chip liquid injection port 80. When the recognition of the liquid injection sleeve 11 and the liquid injection port 80 is coaxial, the vertical moving unit 20 device is lowered, the liquid injection sleeve 11 contacts the liquid injection port 80, the pressure sensor of the liquid injection port 80 returns a signal to confirm the correct docking, and the rotating drive mechanism 3 starts to drive the upper liquid injection sleeve 11 to rotate. The trigger block 510 of the upper liquid injection sleeve 13 rotates out of the locking channel 511 of the liquid injection sleeve 11. The trigger spring 50 is in a compressed state. When the trigger block 510 of the upper liquid injection sleeve 13 moves out of the locking channel 511, the compressed trigger spring 50 is released and pushes the liquid injection sleeve 11 and the liquid injection port 80 tightly. When the system detects that it is tightly connected, the frameless motor continues to rotate to drive the internal threads of the liquid injection sleeve 11 to connect with the external threads of the liquid injection port 80. The elastic sealing sleeve 12 in the liquid injection sleeve 11 is continuously pressed against the liquid injection port 80 to form an interference fit, achieving a certain effect of preventing liquid leakage. The liquid injection tube 10 passes through the elastic sealing sleeve 12 and is inserted into the liquid injection port 80. When the pressure sensor of the liquid injection sleeve 11 detects that the pressure reaches a certain value, the frameless motor stops rotating, the threads of the liquid injection sleeve 11 and the liquid injection port 80 stop rotating and connecting, and the liquid injection step is completed.

[0089] The fifth step is the liquid injection step, as shown in Figure 1 When the liquid injection mechanism 1 is docked, the system will start the air tightness test. The cylinder device of the liquid injection converter starts to move the cylinder rod to dock and press the gas nozzle of the liquid injection tube 10 to the gas nozzle on the reagent tube cover. The converter spring is compressed to ensure the air tightness of the gas nozzle connection. There is a rubber sleeve in the gas nozzle of the liquid injection tube 10 to further ensure the air tightness. A Luer connector is used to connect between the gas pump and the gas delivery hose. The system starts the gas pump to smoothly inject a small amount of gas. The gas pressure sensor in the liquid injection mechanism 1 detects whether the gas pressure is smooth and continuous during the injection of the gas. If it is continuous and smooth, it is determined that the air tightness is good. If the measured value is abnormal, the gas nozzle of the liquid injection tube 10 automatically re-docks. If the air tightness is still abnormal, the operator is prompted to check whether the connection of other parts is normal. When the air tightness test is good, the gas pump starts again to inject liquid. The liquid injection method is set by the operator in advance on the computer or operation screen. The computer can set multiple solution continuous injection mode and single solution injection mode. If the multiple solution injection mode is set, when all the solutions in one reagent tube are injected, the liquid injection mode is adjusted to the cleaning mode. The liquid injection mechanism 1 is disconnected from the liquid injection port 80 and is driven by the horizontal moving unit 21 to the limiting position. Pure water is provided by a special reagent tube to clean the pipeline in the liquid injection mechanism 1. The waste liquid is discharged into the liquid collection tank. The liquid collection tank guide is connected to the external pipeline to discharge the waste liquid. Repeat the fourth step to reconnect the liquid injection port 80. The cylinder device drives the cylinder rod to retreat. The reagent tube holder rotates to the next reagent tube to be injected. There is no need to detect the air tightness to repeat the step of connecting the gas nozzle of the liquid injection tube 10. The above steps can realize fast conversion of reagent injection.

[0090] The sixth step is to separate the liquid injection mechanism 1 from the liquid injection port 80. Figure 1 As shown; when it is detected that the injection is completed, in order to prevent the solution from flowing out in the opposite direction after the injection sleeve 11 is separated, the air pressure sensor device in the injection machine will detect the pressure in the tube. After the pressure returns to normal, the vertical moving unit 20 device moves upward, and the frameless motor rotates in the opposite direction to release the threaded connection between the injection port 80 and the injection sleeve 11 until it is completely separated. The two reset gears 60 are driven by the reduction reset motor 62 to start retracting the injection sleeve 11 and re-tighten the trigger spring 50. After the trigger block 510 reaches the bottom, the injection sleeve 11 is fixed, and the frameless motor continues to rotate in the opposite direction to return to the original limit position. The injection mechanism 1 is taken to the liquid collection tank by the horizontal moving unit 21 device for cleaning and drying the pipeline.

[0091] Example 2

[0092] The second embodiment of this application, such as Figure 10 As shown, it is basically the same as the first embodiment, with the difference that, first, the way the injection tube 10 is assembled and connected in the injection sleeve 11 is different, that is, the injection tube 10 and the injection sleeve 11 are not directly connected by threads, but the injection tube 10 is installed in the injection sleeve 11 through a limit member 15; second, the end of the injection sleeve 11 is not provided with a thread for connecting to the injection port 80.

[0093] In this embodiment, the liquid injection mechanism 1 further includes a liquid injection limiter sleeve 14 and a limiter 15; Figure 10 、 Figure 13 、 Figure 14 As shown, the injection tube 10 is screwed into the injection limiter sleeve 14, and the injection limiter sleeve 14 can be slidably installed in the injection sleeve 11; the limiter 15 abuts the injection limiter sleeve 14, and the limiter 15 is provided at one end of the injection limiter sleeve 14 away from the injection port 80. The limiter 15 is used to keep the injection limiter sleeve 14 moving toward the injection port 80. When in use, the limiter 15 can keep the injection limiter sleeve 14 in the direction of the injection port 80. The elastic sealing sleeve 12 is kept elastically clamped by the injection tube 10 and the injection sleeve 11, thereby preventing the injection tube 10 from loosening from the elastic sealing sleeve 12 during the injection process, thereby causing the injection liquid of the injection tube 10 to flow out from the elastic upper part of the injection sleeve 11 to the outside.

[0094] In this embodiment, if Figure 10 、 Figure 12 、 Figure 13As shown, the limit member 15 includes a limit rod 150 and a limit block 151; the limit rod 150 includes a fixed end and a protruding end, the fixed end is fixedly installed in the injection sleeve 11, and the protruding end is rotatably connected to the limit block 151; the limit block 151 includes a narrow end and a wide end, the narrow end abuts against the injection limit member sleeve 14, and the wide end abuts against the inner wall of the injection sleeve 11, and the distance between the narrow end and the rotation point of the limit block 151 is greater than the distance between the wide end and the rotation point of the limit block 151. When in use, the limit block 151 can limit the movement of the injection limit member sleeve 14, thereby achieving the fixation of the injection tube 10.

[0095] The fixing method of the limiting member 15 used in this embodiment is as follows: Figure 10 As shown, the liquid injection tube 10 can keep moving toward the liquid injection port 80, and the liquid injection sleeve 11 has a constant diameter pipe section for limiting the movement of the liquid injection tube 10. Compared with the ordinary fixing method, when the liquid injection port 80 abuts against the elastic sealing sleeve 12, the elastic sealing sleeve 12 can not only be subjected to the upward force applied by the liquid injection port 80, but also the elastic sealing sleeve 12 can be subjected to the continuous downward force applied by the liquid injection tube 10. The elastic sealing sleeve 12 abuts against the liquid injection tube 10 and the liquid injection port 80 more tightly, so that the interference seal fit effect is better.

[0096] Example 3

[0097] The third embodiment of this application, such as Figures 11 to 13 As shown, it is basically the same as the second embodiment, except that the liquid injection mechanism 1 is not provided with a buffer reset member 16.

[0098] In this embodiment, if Figures 11 to 13 As shown, the injection mechanism 1 also includes a buffer reset member 16, which is arranged in the injection sleeve 11. The buffer reset member 16 is used to provide a buffer for the injection sleeve 11 to prevent the injection sleeve 11 from moving excessively when connected to the injection port 80, resulting in damage to the injection sleeve 11.

[0099] like Figures 11 to 13As shown, the buffer reset member 16 includes a buffer reset spring 160 and a buffer reset sleeve 161. The buffer reset sleeve 161 is inserted into the injection sleeve 11, and the buffer reset sleeve 161 can move in the axial direction of the injection sleeve 11. A spring pressure block 1115 is provided on the inner wall of the end of the injection sleeve 11 away from the injection port 80. The buffer reset spring 160 is compressed between the spring pressure block and one end of the buffer reset sleeve 161, and the other end of the buffer reset sleeve 161 can move in or out of the injection sleeve 11. During use, when the injection sleeve 11 moves into the injection port 80, the buffer reset sleeve 161 first abuts against the microfluidic chip, and then the buffer reset sleeve 161 slides upward, thereby compressing the reset spring, thereby realizing a buffering function during the connection process, avoiding excessive movement and causing damage to the injection sleeve 11 or the injection tube 10; when the injection sleeve 11 is moved out to the injection port 80, the reset spring can realize the reset of the buffer reset sleeve 161, which is helpful for the next use.

[0100] In this embodiment, for the buffer reduction sleeve 161, Figures 11 to 13 As shown, the buffer reset sleeve 161 includes a buffer reset rod 1610 and a buffer reset block 1611. The buffer reset rod 1610 can be slidably installed in the injection sleeve 11. Both ends of the buffer reset rod 1610 are connected to the buffer reset block 1611. The reset block at one end serves to abut against the microfluidic chip, and the reset block at the other end is used to abut against the buffer reset spring 160.

[0101] The buffer reset member 16 used in this embodiment can prevent the injection sleeve 11 or the injection tube 10 from moving excessively during the movement and connection process of the injection mechanism 1, which may cause the injection sleeve 11 or the injection tube 10 to directly collide with the microfluidic chip and cause damage. It can effectively protect the connection process of the injection mechanism 1 and improve the safety of the injection mechanism 1.

[0102] Example 4

[0103] The fourth embodiment of the present application is as follows: Figure 14 As shown, it is basically the same as the third embodiment, with the differences being that, first, the elastic sealing sleeve 12 is not provided with a thick diameter wall section 120, a variable diameter wall section 121 and a narrow diameter wall section 122, but the elastic sealing sleeve 12 is an airbag; second, the injection tube 10 is not provided with an injection variable diameter section 100, but is entirely a constant diameter tube.

[0104] In this embodiment, the elastic sealing sleeve 12 is an airbag, which is equipped with an air inlet pipe extending out of the injection sleeve 11. When in use, the airbag is inflated through the air inlet pipe. The airbag filled with gas can connect the injection tube 10, the injection sleeve 11 and the injection port 80 in an interference sealing manner to achieve sealing.

[0105] In the embodiment, the liquid injection pipe 10 is a constant diameter pipe, and the liquid injection pipe 10 is installed in the liquid injection sleeve 11 through the limiting member 15.

[0106] The above is the preferred embodiment of the present application, it should be noted that for those skilled in the art, without departing from the principles of the present application, can also make several improvements and refinements, these improvements and refinements are also considered the scope of protection of the present application.

Claims

1. An automated microfluidic chip injection device, characterized in that: include Liquid injection mechanism, moving mechanism and rotation drive mechanism; The liquid injection mechanism includes a liquid injection tube, a liquid injection sleeve, an elastic sealing sleeve and an upper liquid injection sleeve; The injection tube is inserted into the injection sleeve, and the elastic sealing sleeve is elastically clamped between the injection tube and the injection sleeve, and the elastic sealing sleeve is arranged at the injection insertion end of the injection tube; The injection sleeve is rotatably connected to the upper injection sleeve, and the rotation of the upper injection sleeve is used to move the injection sleeve away from the upper injection sleeve; The rotation drive mechanism is installed on the injection mechanism, and the rotation drive mechanism is used to drive the upper injection sleeve to rotate; The liquid injection mechanism is installed on the moving mechanism, and the moving mechanism is used to drive the liquid injection mechanism to move so that the liquid injection tube moves into or out of the liquid injection port of the microfluidic chip and the elastic sealing sleeve is compressed between the liquid injection port and the liquid injection sleeve; When the liquid injection sleeve abuts against the liquid injection port, the elastic sealing sleeve is compressed by the liquid injection sleeve and the liquid injection port, and the elastic sealing sleeve realizes interference sealing cooperation with the liquid injection port and the liquid injection sleeve; The liquid injection mechanism also includes a liquid injection limiter sleeve and a limiter; The injection pipe is threadedly connected to the injection limiter sleeve, and the injection limiter sleeve is slidably installed in the injection sleeve; The limiting member abuts against the liquid injection limiting member sleeve, and the limiting member is provided at one end of the liquid injection limiting member sleeve away from the liquid injection port, and the limiting member is used to keep the liquid injection limiting member sleeve moving toward the liquid injection port; The limiting member includes a limiting rod and a limiting block; The limiting rod includes a fixed end and an extended end, the fixed end is fixedly installed in the injection sleeve, and the extended end is rotatably connected to the limiting block; The limit block includes a narrow end and a wide end, the narrow end abuts against the injection limit member sleeve, the wide end abuts against the inner wall of the injection sleeve, and the distance between the narrow end and the rotation point of the limit block is greater than the distance between the wide end and the rotation point of the limit block; The automated microfluidic chip injection device further includes a trigger mechanism; The trigger mechanism includes a trigger spring and a trigger locking member; The upper injection sleeve is coaxially arranged with the injection sleeve, and a compression convex wall is provided on the outer wall of the upper injection sleeve, and the compression convex wall is provided outside the injection sleeve; The trigger spring is compressed between the compression convex wall and the injection sleeve; The trigger locking member is provided between the upper liquid injection sleeve and the liquid injection sleeve; The upper liquid filling sleeve is rotated to put the trigger locking member into an unlocked state or a locked state; When the trigger locking member is in an unlocked state, the trigger spring is used to push the injection sleeve to move away from the upper injection sleeve; When the trigger locking member is in a locked state, the injection sleeve is clamped in the upper injection sleeve; The trigger locking member includes a trigger block, a locking channel and a trigger channel; The trigger block is arranged on the outer wall of the upper injection sleeve; The locking channel and the triggering channel are provided on the inner wall of the injection sleeve, and the locking channel and the triggering channel are communicated with each other; The locking channel is circumferentially arranged around the outer wall of the upper injection sleeve, and the axial direction of the trigger channel is the same as the axial direction of the upper injection sleeve; The trigger block can be slidably installed in the locking channel or the trigger channel.

2. The automated microfluidic chip injection device according to claim 1, characterized in that: The injection sleeve is provided with a first constant diameter pipe section; The injection pipe is provided with an injection diameter-changing section, which penetrates into the first constant-diameter pipe section. Along the direction adjacent to the injection port, the diameter of the injection diameter-changing section gradually decreases, and the maximum diameter of the injection diameter-changing section is greater than the diameter of the first constant-diameter pipe section.

3. The automated microfluidic chip injection device according to claim 1, characterized in that: The injection sleeve is further provided with a second constant diameter pipe section and a variable diameter pipe section; Along the direction adjacent to the liquid injection port, the variable diameter pipe section and the second constant diameter pipe section are connected in sequence, and the diameter of the variable diameter pipe section gradually increases; The elastic sealing sleeve is arranged in the second constant diameter pipe section and / or the variable diameter pipe section, and the sleeve diameter of the elastic sealing sleeve is larger than the minimum pipe diameter of the variable diameter pipe section.

4. The automated microfluidic chip injection device according to claim 1, characterized in that: The elastic sealing sleeve comprises a thick diameter wall section, a variable diameter wall section and a narrow diameter wall section; Along the direction adjacent to the liquid injection port, the thick-diameter wall section, the variable-diameter wall section, and the narrow-diameter wall section are connected in sequence; The outer wall of the thick-wall section elastically abuts against the inner wall of the injection sleeve; The inner wall of the thick-diameter wall section, the inner wall of the variable-diameter wall section, and the inner wall of the narrow-diameter wall section are all in elastic contact with the outer wall of the liquid injection tube.

5. The automated microfluidic chip injection device according to claim 1, characterized in that: The liquid injection mechanism also includes a buffer reset component; The inner wall of the injection sleeve is provided with a spring pressure block; The buffer reset member includes a buffer reset spring and a buffer reset sleeve; The buffer reset sleeve is axially movable and penetrates the liquid injection sleeve. The reset spring is compressed between one end of the buffer reset sleeve away from the liquid injection port and the spring pressure block. The other end of the buffer reset sleeve can be moved out of or into the liquid injection sleeve.

6. The automated microfluidic chip injection device according to claim 1, characterized in that: The automated microfluidic chip injection device further includes a chip clamping mechanism; The chip clamping mechanism includes a leveling component, a placement table and a plurality of chip clamping members; The placement table is installed on the leveling component, and the leveling component is used to level the placement table; The plurality of chip clamps are relatively arranged along the same axis, and the chip clamps include a clamping portion and a driving portion; The clamping part is arranged on the placement table, and is in transmission connection with the driving part. The driving part is used to drive the clamping part to move away from or closer to the microfluidic chip to clamp the microfluidic chip.

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