An acne-removing composition, a preparation method, application and cosmetic thereof

Abstract

The application relates to a kind of acne-removing compositions and its preparation method, application, cosmetics.The acne-removing composition comprises: coptis chinensis, magnolia officinalis extract and costus root extract, the coptis chinensis and magnolia officinalis extract are extracted from the mixture of coptis chinensis and magnolia officinalis as extraction raw material, and the costus root extract is extracted from costus root as extraction raw material.The acne-removing composition provided in the application can inhibit sebaceous gland excessive secretion of oil and inhibit the growth of propionibacterium acnes, thereby can reduce skin inflammation and infection, and play the effect of preventing and treating acne.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and specifically to an anti-acne composition and its preparation method, application, and cosmetics. Background Art

[0002] Acne is a chronic inflammatory skin disease of the pilosebaceous unit, commonly presenting during adolescence. Clinical manifestations include facial acne, papules, pustules, nodules, and other polymorphic skin lesions. The pathogenesis of acne involves keratinization of the hair follicles, which blocks oil secretion. This leads to increased oil secretion and accumulation within the follicles and sebaceous glands, creating a breeding ground for Propionibacterium acnes. When pores become clogged, they rapidly grow, breaking down saturated fatty acids and producing large quantities of free fatty acids. These fatty acids penetrate the skin through the pores, triggering a stress response and causing acne, redness, and swelling. Bacteria such as Staphylococcus attach to the skin through the released pus, damaging skin tissue and ultimately allowing a proliferation of bacteria. This in turn triggers inflammation of the hair follicles and sebaceous glands. Malassezia, in particular, increases the secretion of IL-1β, IL-6, IL-8, and TNF-α by keratinocytes, while decreasing IL-10 secretion, exacerbating the inflammatory response and potentially leading to disfiguring scarring. Therefore, how to effectively prevent and treat acne is still a problem that needs to be solved urgently. Summary of the Invention

[0003] In response to the problems existing in the prior art, the embodiments of the present application provide an acne-removing composition and its preparation method, application, and cosmetics, in order to effectively prevent or treat acne.

[0004] In a first aspect, the present invention provides an anti-acne composition comprising:

[0005] Coptis chinensis and Magnolia bark extract: extracted from a mixture of Coptis chinensis and Magnolia bark;

[0006] Costusroot extract: extracted from Costusroot as the raw material.

[0007] In some embodiments, the mass ratio of the extraction raw materials Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:(0.8-1.2):(0.8-1.2); in some embodiments, the mass ratio of the extraction raw materials Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:1:1.

[0008] In a second aspect, the present invention provides a method for preparing an anti-acne composition, comprising the following steps:

[0009] Mixing Rhizoma Coptidis and Rhizoma Magnoliae and performing subcritical extraction to obtain a first extract;

[0010] Cooling the first extract to crystallize and then drying to obtain a first extract product;

[0011] Mixing the costusroot and an organic base for extraction to obtain a second extract;

[0012] subjecting the second extract to resin adsorption and then desorption with a polyol aqueous solution to obtain a desorbed liquid;

[0013] The desorption liquid is mixed with the first extraction product to obtain the anti-acne composition.

[0014] In some embodiments, the mass ratio of Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:(0.8-1.2):(0.8-1.2); in some embodiments, the mass ratio of the extraction raw materials Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:1:1.

[0015] In some embodiments, the step of mixing Rhizoma Coptidis and Rhizoma Magnoliae Bark and performing subcritical extraction to obtain a first extract comprises:

[0016] The coptis root and magnolia bark are mixed and crushed, put into a subcritical extraction tank, added with deionized water for high temperature and high pressure extraction, and filtered to obtain a first extract; wherein,

[0017] In some embodiments, the extraction temperature of the high temperature and high pressure extraction is 105° C. to 120° C., and the extraction pressure is 1.3 to 1.5 standard atmospheres.

[0018] In some embodiments, extracting the mixed Radix Aucklandiae Root and the organic base to obtain the second extract comprises:

[0019] Crush the woodruff, add organic alkali and deionized water to perform low-temperature extraction, and filter to obtain a second extract after extraction; wherein,

[0020] In some embodiments, the extraction temperature of the low-temperature extraction is 40°C to 55°C.

[0021] In some embodiments, the organic base comprises one or more of betaine, L-carnitine, and stachydrine.

[0022] In some embodiments, the mass ratio of the Sichuan costus root to the organic base is 1:(0.08-0.12).

[0023] In some embodiments, the resin used for the resin adsorption includes one or both of polyamide resin and snake cage resin.

[0024] In some embodiments, the polyol includes one or more of glycerol, 1,3-propylene glycol, 1,3-butylene glycol, 1,2-propylene glycol, dipropylene glycol, and methylpropylene glycol.

[0025] In some embodiments, the mass concentration of the polyol aqueous solution is 55% to 65%.

[0026] In some embodiments, the mass ratio of the Sichuan costus root to the polyol aqueous solution is 1:(11.76-16.66).

[0027] In a third aspect, the embodiments of the present application provide the use of the anti-acne composition of the embodiment of the first aspect of the present application or the anti-acne composition obtained by the preparation method of the embodiment of the second aspect of the present application in anti-acne cosmetics.

[0028] In a fourth aspect, the embodiments of the present application provide a cosmetic comprising the anti-acne composition of the first aspect of the present application or the anti-acne composition obtained by the preparation method of the second aspect of the present application.

[0029] In some embodiments, the mass percentage of the anti-acne composition in the cosmetic is 1% to 3%. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0031] Figure 1 These are the results of the HaCaT cell toxicity experiment in the examples of this application.

[0032] Figure 2 This is the IOD value result of the sebaceous gland cell oil control test in the embodiment of this application.

[0033] Figure 3 This is a fluorescence image of the oil control effect of sebaceous gland cells in the examples of this application.

[0034] Figure 4 This is the result of the clinical acne skin redness reduction test in the examples of this application.

[0035] Figure 5 These are the results of a clinical acne volume change test in the examples of this application.

[0036] Figure 6 These are the results of a clinical skin oil content change test in the examples of this application.

[0037] Figure 7 These are the results of a clinical transepidermal water loss test in the examples of this application.

[0038] Figure 8 This is a VISA collection diagram for clinical acne treatment in an embodiment of the present application (the darker the color, the higher the relative height of the acne). DETAILED DESCRIPTION

[0039] The features and exemplary embodiments of various aspects of the present application will be described in detail below. In the detailed description below, many specific details are set forth in order to provide a comprehensive understanding of the present application. However, it will be apparent to those skilled in the art that the present application can be implemented without some of these specific details. The following description of the embodiments is merely intended to provide a better understanding of the present application by illustrating examples of the present application. In the following description, at least some of the well-known structures and technologies are not shown in order to avoid unnecessary ambiguity in the present application; and, for clarity, the sizes of some structures may be exaggerated. In addition, the features, structures, or characteristics described below may be combined in any suitable manner in one or more embodiments.

[0040] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish an entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. In the description of the present application, it should be noted that, unless otherwise stated, the meaning of "plurality" is more than two. Moreover, the term "comprises", "comprising" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment comprising a series of elements not only include those elements, but also include other elements not clearly listed, or also include elements inherent to such process, method, article or equipment. In the absence of more restrictions, the elements limited by the statement "comprises..." do not exclude the presence of other identical elements in the process, method, article or equipment comprising the elements.

[0041] As described in the background, modern medical treatments for acne primarily include oral antibiotics, retinoic acid, sex hormones, and benzoyl peroxide. Retinoic acid, a first-line treatment, has been shown to have anti-keratotic and anti-inflammatory effects. However, these drugs currently suffer from drawbacks such as being unsuitable for long-term use, exhibiting diverse adverse reactions, and being prone to developing drug resistance.

[0042] Traditional Chinese Medicine (TCM) offers significant advantages in treating acne. Through preparation and combination, drug toxicity can be reduced, while formulation improves effectiveness and specificity. Consequently, TCM compositions offer minimal irritation, few side effects, and high efficacy. These compositions can reduce the incidence of infections and serious complications associated with long-term steroid use. Furthermore, TCM has a beneficial effect on regulating the body's immune system. Therefore, research into the prevention and treatment of acne with traditional Chinese medicine offers a new avenue for the discovery of modern therapeutic compositions. Currently, TCM formulas for acne prevention are generally ineffective, resulting in slow acne treatment and recurring attacks.

[0043] Acne syndromes can be divided into three types: internal warm-heat syndrome, lung meridian wind-heat syndrome, and liver depression and qi stagnation. Treatment for the internal warm-heat syndrome focuses on clearing heat and detoxifying, promoting bowel movement and removing dampness. Treatment for the lung meridian wind-heat syndrome primarily involves purging the lungs, clearing heat, cooling the blood, and detoxifying. Treatment for the liver depression and qi stagnation syndrome focuses on soothing the liver and relieving depression, and harmonizing the liver and spleen. Dietary, emotional, and skincare adjustments should also be considered. Clinical manifestations include facial flushing, red, swollen, and painful acne, or pustules, accompanied by pain. Coptis chinensis (Coptis chinensis) belongs to the Ranunculaceae family and is bitter and cold in nature. It enters the heart, liver, stomach, and large intestine meridians, and has the effects of clearing heat and dampness, purging fire, and detoxifying. Costus root (Aucklandia lappa) belongs to the Asteraceae family and is pungent and warm in nature. It enters the liver, spleen, and stomach meridians, and has aromatic, stomach-strengthening, qi-invigorating, and pain-relieving properties. Magnolia bark (Magnolia officinalis) belongs to the Magnoliaceae family and is pungent and warm in nature. It enters the spleen, stomach, and large intestine meridians, and has the effects of drying dampness. Currently, there are no studies or reports on the combined use of Coptis chinensis, Magnolia bark, and Costus root in the treatment of acne.

[0044] Acne-removing composition

[0045] The embodiment of the first aspect of the present application provides an acne-removing composition comprising:

[0046] Coptis chinensis and Magnolia bark extract: extracted from a mixture of Coptis chinensis and Magnolia bark;

[0047] Costusroot extract: extracted from Costusroot as the raw material.

[0048] The acne-removing composition comprises a mixed extract of coptis chinensis and magnolia bark, and an extract of kaempferia sinensis. The combination of these extracts can inhibit excessive oil secretion from sebaceous glands and the growth of Propionibacterium acnes, thereby reducing skin inflammation and infection, thereby preventing and treating acne. Furthermore, the acne-removing composition provided in the embodiments of the present application is prepared and combined with traditional Chinese medicine components, resulting in low irritation and few side effects. It can reduce the incidence of infections and complications caused by long-term hormone use. Traditional Chinese medicine also has a good regulatory effect on the human immune function and can repair the skin barrier. Therefore, it can be well applied to acne-removing cosmetics or acne treatment drugs.

[0049] In some embodiments, the mass ratio of the extraction raw materials Rhizoma Coptidis, Magnolia Bark and Radix Aucklandiae is 1:(0.8-1.2):(0.8-1.2).

[0050] In some embodiments, the mass ratio of the extraction raw materials Coptidis Rhizoma, Magnolia Bark and Aucklandia Rhizoma is 1:1:1.

[0051] By limiting the ratio of each extraction raw material to the above range, the obtained extract can have a better compatibility effect, further improving the efficacy of the acne-removing composition.

[0052] Preparation method of anti-acne composition

[0053] The second embodiment of the present application provides a method for preparing an acne-removing composition, comprising the following steps:

[0054] S100, performing subcritical extraction on a mixture of coptis chinensis and magnolia bark to obtain a first extract;

[0055] S200, cooling and crystallizing the first extract and then drying it to obtain a first extract product;

[0056] S300, extracting the Radix Aucklandiae lappa and an organic base to obtain a second extract;

[0057] S400, subjecting the second extract to resin adsorption and then desorption with a polyol aqueous solution to obtain a desorption solution;

[0058] S500, mixing the desorption liquid with the first extraction product to obtain the acne-removing composition.

[0059] In some embodiments, the mass ratio of Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:(0.8-1.2):(0.8-1.2); in some embodiments, the mass ratio of the extraction raw materials Coptis chinensis, Magnolia officinalis and Aucklandia lappa is 1:1:1.

[0060] By limiting the mass ratio of Coptis chinensis, Magnolia officinalis and Costus root to the above range, the mixed extraction effect of Coptis chinensis and Magnolia officinalis is better, and the purity and extraction rate of the extract can be further improved.

[0061] In some embodiments, step S100 includes:

[0062] The coptis chinensis and magnolia bark are mixed and crushed, and the mixture is put into a subcritical extraction tank, deionized water is added for high temperature and high pressure extraction, and the first extract is obtained by filtering.

[0063] Coptis chinensis contains a large amount of alkaloid components such as berberine, coptisine, methyl coptisine, and palmatine, while Magnolia bark contains a large amount of the active ingredient magnolol. When Coptis chinensis and Magnolia bark are crushed and mixed for extraction, the alkaloids in Coptis chinensis can act as ion donors, and the magnolol compounds in Magnolia bark act as ion acceptors. The two can form a cocrystal to form a supramolecular compound crystal, thereby improving the extraction efficiency and product purity of Coptis chinensis and Magnolia bark, and obtaining a high-purity crystalline extract. The obtained crystals are filtered and dried to obtain the first extraction product.

[0064] In some embodiments, the extraction temperature of the high temperature and high pressure extraction may be 105° C. to 120° C., and the extraction pressure may be 1.3 to 1.5 standard atmospheric pressures. In this application, standard atmospheric pressure is 101.325 kPa.

[0065] Limiting the extraction temperature and extraction pressure of Coptis chinensis and Magnolia officinalis to the above ranges can improve the extraction efficiency and effectiveness, and increase the concentration of active ingredients in the extract.

[0066] In some embodiments, step S300 includes: crushing Costus root, adding organic base and deionized water for low-temperature extraction, and filtering after extraction to obtain a second extract.

[0067] Kaohsiung wood contains a large number of flavonoid active ingredients. When Kaohsiung wood is crushed and mixed with an organic base for extraction, the flavonoids in Kaohsiung wood can act as ion receptors and the organic base as ion donors. The two can form a supramolecular compound crystal, thereby extracting the active ingredients in Kaohsiung wood. This can improve the efficiency of Kaohsiung wood extraction and the purity of the product. In the subsequent resin adsorption process, since the adsorption resin does not adsorb the organic base, the organic base in the second extract can be separated from the Kaohsiung wood extract.

[0068] In some embodiments, the extraction temperature of the low-temperature extraction may be 40° C. to 55° C. Within this extraction temperature range, the purity and extraction efficiency of the extracted product can be improved.

[0069] In some embodiments, the organic base includes one or more of betaine, L-carnitine, and stachydrine.

[0070] In some embodiments, the mass ratio of Sichuan costus root to the organic base can be 1: (0.08-0.12).

[0071] The above-mentioned organic base can form supramolecular compound crystals with the flavonoid active ingredients in Sichuan costus root, which can further improve the extraction efficiency and the purity of the extracted product.

[0072] The second extract is subjected to resin adsorption to remove impurities such as organic bases. The resin used for adsorption can be one or more of polyamide resin and snake cage resin. The adsorption resin effectively adsorbs the flavonoid active ingredients in the second extract while barely or only slightly adsorbing the organic base. Therefore, it can be used for the adsorptive separation of the active ingredients and the organic base in the second extract.

[0073] The active ingredients adsorbed by the adsorption resin are eluted with a polyol, which may include one or more of glycerol, 1,3-propylene glycol, 1,3-butylene glycol, 1,2-propylene glycol, dipropylene glycol, and methyl propylene glycol.

[0074] The polyol can act as a solvent in the anti-acne composition, thereby uniformly dispersing the components and improving the contact of the extract components in the anti-acne composition with the skin surface.

[0075] In some embodiments, the mass concentration of the polyol aqueous solution may be 55% to 65%.

[0076] Application of anti-acne composition in anti-acne cosmetics

[0077] The acne-removing composition provided in the first embodiment of the present application or the acne-removing composition provided according to the preparation method of the second embodiment of the present application has good effects of inhibiting excessive oil secretion by sebaceous glands, inhibiting the growth of Propionibacterium acnes, and reducing skin inflammation and infection, and therefore can be used in acne-removing cosmetics.

[0078] cosmetic

[0079] Another embodiment of the present application provides a cosmetic comprising the anti-acne composition of the first embodiment of the present application or the anti-acne composition obtained by the preparation method of the second embodiment of the present application.

[0080] In some embodiments, the mass percentage of the anti-acne composition in the cosmetic may be 1% to 3%.

[0081] Example

[0082] The present invention is described below with reference to specific examples. It should be noted that the examples described below are exemplary and are intended only to explain the present application, and should not be construed as limiting the present application. Where specific techniques or conditions are not specified in the examples, the techniques or conditions described in the literature in this area or the product specifications are used. Reagents or instruments used without manufacturer's indication are all commercially available conventional products.

[0083] Example 1

[0084] An anti-acne composition is prepared by the following method:

[0085] S100: 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0086] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.75 kg of L-carnitine were added, and the mixture was extracted at 50 ° C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtering with a 500-mesh filter bag. The resin was eluted with 100 kg of a 60% 1,3-propylene glycol back water solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag, and the filtrate was collected;

[0087] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0088] Example 2

[0089] An anti-acne composition is prepared by the following method:

[0090] S100: 6 kg of Coptis chinensis and 6 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0091] S200, 6 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.65 kg of L-carnitine were added, and the mixture was extracted at 50°C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtration using a 500-mesh filter bag. The resin was eluted using 100 kg of a 60% 1,3-propylene glycol aqueous solution for 1 h at a temperature of 55°C. The eluate was filtered using a 500-mesh filter bag, and the filtrate was collected;

[0092] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0093] Example 3

[0094] An anti-acne composition is prepared by the following method:

[0095] S100: 8.5 kg of Coptis chinensis and 8.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0096] S200, 8.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 0.85 kg of L-carnitine, extracted at 50 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-butanediol aqueous solution for 1 h at 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0097] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0098] Example 4

[0099] An anti-acne composition is prepared by the following method:

[0100] S100: 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added and extracted at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The extract was allowed to crystallize for 24 h, and the crystals were collected by filtration.

[0101] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 0.75 kg of betaine, extracted at 50 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0102] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0103] Example 5

[0104] An anti-acne composition is prepared by the following method:

[0105] S100: 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times the standard atmospheric pressure for 2 h. The extract was cooled to below 95°C, filtered through a 0.45 μm nylon membrane, and allowed to crystallize for 24 h. The crystals were then collected by filtration.

[0106] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.75 kg of L-carnitine were added, and the mixture was extracted at 50 ° C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtering with a 500-mesh filter bag. The resin was eluted with 120 kg of a 65% 1,3-propylene glycol aqueous solution for 1 h at a temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0107] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0108] Example 6

[0109] An anti-acne composition is prepared by the following method:

[0110] S100: 7.5 kg of Coptis chinensis and 9.0 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times the standard atmospheric pressure for 2 h. The extract was cooled to below 95°C, filtered through a 0.45 μm nylon membrane, and allowed to crystallize for 24 h. The crystals were then collected by filtration.

[0111] S200, 9.0 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.75 kg of L-carnitine were added, and the mixture was extracted at 50°C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtering with a 500-mesh filter bag. The resin was eluted with 90 kg of a 55% 1,3-propylene glycol aqueous solution for 1 h at a temperature of 55°C. The eluate was filtered with a 500-mesh filter bag, and the filtrate was collected;

[0112] S300, adding the crystals collected in step S100 to the filtrate collected in step S200, stirring and dissolving at 60°C for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain an anti-acne composition.

[0113] Comparative Example 1

[0114] S100: 7.5 kg each of Coptis chinensis and Magnolia officinalis were crushed, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added and extracted at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The crystals were allowed to crystallize for 24 h and then collected by filtration.

[0115] S200 , adding the crystals collected in step S100 into 100 kg of a 60% 1,3-propylene glycol aqueous solution, stirring and dissolving at 60° C. for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain a comparative composition 1.

[0116] Comparative Example 2

[0117] S100: 7.5 kg of Coptis chinensis was crushed, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 1700 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times the standard atmospheric pressure for 2 h. The extract was cooled to below 95°C, filtered through a 0.45 μm nylon membrane, and allowed to crystallize for 24 h. The crystals were then collected by filtration.

[0118] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 0.75 kg of L-carnitine, extracted at 90 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 760% 1,3-butanediol for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0119] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 2.

[0120] Comparative Example 3

[0121] S100: 7.5 kg of Magnolia officinalis was crushed, passed through a 60-mesh sieve, and then added with 100 kg of deionized water for high-temperature extraction for 2 h. The mixture was filtered through a 0.45 μm nylon membrane, and the filtrate was allowed to crystallize for 24 h. The crystals were collected by filtration.

[0122] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.75 kg of L-carnitine were added, and the mixture was extracted at 90 ° C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtration using a 500-mesh filter bag. The resin was eluted using 100 kg of a 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered using a 500-mesh filter bag, and the filtrate was collected;

[0123] S300 , adding the crystals collected in step S100 to the filtrate of S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 3.

[0124] Comparative Example 4

[0125] S100: Grind 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis, pass through a 60-mesh sieve, add 100 kg of deionized water, extract at 100°C for 2 h, and filter through a 5 μm polypropylene membrane; let the filtrate stand for 24 h to crystallize, and collect the crystals by suction;

[0126] S200 , adding the crystals collected in step S100 into 100 kg of a 60% 1,3-propylene glycol aqueous solution, stirring and dissolving at 60° C. for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 4.

[0127] Comparative Example 5

[0128] S100: Grind 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis, pass through a 60-mesh sieve, add 100 kg of deionized water, extract at 100°C for 2 h, and filter through a 5 μm polypropylene membrane; let the filtrate stand for 24 h to crystallize, and collect the crystals by suction;

[0129] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.75 kg of L-carnitine were added, and the mixture was extracted at 90 ° C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtration using a 500-mesh filter bag. The resin was eluted using 100 kg of a 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered using a 500-mesh filter bag, and the filtrate was collected;

[0130] S300 , adding the crystals collected in step S100 to the filtrate of S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 5.

[0131] Comparative Example 6

[0132] S100: Grind 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis, pass through a 60-mesh sieve, and place in a subcritical extraction tank. Add 100 kg of deionized water and extract at 125°C and 1.5 times standard atmospheric pressure for 2 h. Cool the extract to below 95°C, filter through a 0.45 μm nylon membrane, allow to crystallize for 24 h, and collect the crystals by suction.

[0133] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water, extracted at 90 ° C for 2 h, filtered the extract, collected the filtrate, added it to the polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag, and the filtrate was collected;

[0134] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 6.

[0135] Comparative Example 7

[0136] S100: 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis were crushed, added with 100 kg of deionized water and extracted at 100°C for 2 h. The mixture was filtered through a 0.45 μm nylon membrane, and the filtrate was allowed to crystallize for 24 h. The crystals were then collected by filtration.

[0137] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water, extracted at 90 ° C for 2 h, filtered the extract, collected the filtrate, added it to the polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag, and the filtrate was collected;

[0138] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 7.

[0139] Comparative Example 8

[0140] A composition is prepared by the following method:

[0141] S100: 5.5 kg of Coptis chinensis and 5.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times the standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0142] S200, 5.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 0.55 kg of L-carnitine, extracted at 50 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0143] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 8.

[0144] Comparative Example 9

[0145] A composition is prepared by the following method:

[0146] S100: 9.0 kg of Coptis chinensis and 9.0 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0147] S200, 9.0 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 1.08 kg of L-carnitine, extracted at 50 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0148] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 9.

[0149] Comparative Example 10

[0150] A composition is prepared by the following method:

[0151] S100: 7.5 kg of Coptis chinensis and 10 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added and extracted at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0152] S200, 5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, added with 100 kg of deionized water and 0.4 kg of L-carnitine, extracted at 50 ° C for 2 h, filtered the extract, collected the filtrate and added it to polyamide resin for static adsorption, filtered with a 500-mesh filter bag to collect the resin, and eluted the resin with 100 kg of 60% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag and the filtrate was collected;

[0153] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 h to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 10.

[0154] Comparative Example 11

[0155] A composition is prepared by the following method:

[0156] S100: 7.5 kg of Coptis chinensis and 7.5 kg of Magnolia officinalis were mixed and ground, passed through a 60-mesh sieve, and placed in a subcritical extraction tank. 100 kg of deionized water was added, and extraction was carried out at 125°C and 1.5 times standard atmospheric pressure for 2 h. The extract was cooled to below 95°C and filtered through a 0.45 μm nylon membrane. The filtrate was allowed to crystallize for 24 h, and the crystals were collected by suction filtration.

[0157] S200, 7.5 kg of Sichuan costus root was crushed, passed through a 60-mesh sieve, 100 kg of deionized water and 0.5 kg of L-carnitine were added, and the mixture was extracted at 60 ° C for 2 h. The extract was filtered, and the filtrate was collected and added to a polyamide resin for static adsorption. The resin was collected by filtering with a 500-mesh filter bag. The resin was eluted with 130 kg of a 70% 1,3-propylene glycol aqueous solution for 1 h at an elution temperature of 55 ° C. The eluate was filtered with a 500-mesh filter bag, and the filtrate was collected;

[0158] S300 , adding the crystals collected in step S100 to the filtrate collected in step S200 , stirring and dissolving at 60° C. for 1 hour to form a transparent solution, and filtering with a 0.45 μm nylon membrane to obtain comparative composition 11.

[0159] Test section

[0160] 1. Appearance and stability test

[0161] The appearance of the compositions prepared in each example and comparative example was observed, and their stability after being placed in different environments for 3 months was recorded, as shown in Table 1.

[0162] Table 1

[0163]

[0164] According to the test results in Table 1, the color of the composition obtained by the traditional water extraction method or when the amount of the extracted raw materials is too much is generally dark yellow, and the composition obtained when the content of the Sichuan costus root extract is high has a pungent odor, and the odor is large. The composition of the mixed extract of Coptis chinensis and Magnolia bark and the Sichuan costus root extract of the present application has no pungent odor, which is very important for the anti-acne composition used as a cosmetic raw material. In addition, the addition of Sichuan costus root extract can improve the stability of the mixed extract of Coptis chinensis and Magnolia bark, and reduce the discoloration and precipitation of the extract under high temperature or light conditions. And the composition obtained by extracting using subcritical extraction technology has the advantages of lighter odor and better stability compared to the composition extracted by traditional water extraction technology.

[0165] 2. Micromicrobic Inhibitory Concentration (MIC) for Propionibacterium acnes

[0166] Test Method: Sample solutions obtained by diluting the anti-acne composition at different times were added to corresponding sterile 96-well plates, with 10 μL of sample solution added to each well. One well was reserved for a blank control and then 10 μL of sterile distilled water was added. A bacterial suspension with a concentration equivalent to a 0.5 McFarland turbidimetric standard was mixed with LB medium at a volume ratio of 1:1000 to obtain a dilution. 90 μL of this dilution was added to the sample solution in the wells. The 96-well plates were inoculated with Propionibacterium acnes, sealed, and incubated on a shaker at 37°C for 18 hours before evaluating the results. If bacterial growth was evident in the blank control, the lowest sample concentration that completely inhibited bacterial growth in the remaining wells was determined as the MIC (OD600, measured using a microplate reader; MIC units are weight %). See Table 2 for detailed data.

[0167] Table 2

[0168]

[0169]

[0170] As can be seen from the MIC test results in Table 2, when the extraction raw materials only consist of the combination of Rhizoma Coptidis and Radix Aucklandiae (Comparative Example 2) or only the combination of Cortex Magnoliae Bark and Cortex Aucklandiae (Comparative Example 3), the minimum inhibitory concentration values ​​of the compositions obtained are higher. When the extraction raw materials only consist of the combination of Rhizoma Coptidis and Cortex Magnoliae Bark (Comparative Example 1), the minimum inhibitory concentration values ​​are reduced, indicating that the antibacterial ability of the Rhizoma Coptidis and Cortex Magnoliae Bark extracts is stronger. In each embodiment, the compositions extracted using Rhizoma Coptidis, Cortex Magnoliae Bark and Cortex Aucklandiae have significantly decreased minimum inhibitory concentration values, and the antibacterial efficacy is optimal.

[0171] From the MIC results of Comparative Examples 6 and 7, when Coptidis Rhizoma and Magnolia Bark were extracted using subcritical technology (Comparative Example 6), the antibacterial effect was better than that of Coptidis Rhizoma and Magnolia Bark extracted using traditional technology (Comparative Example 7); when Radix Aucklandiae Radix was extracted using a low eutectic solvent (Comparative Example 5), its antibacterial effect was better than that of traditional water-extracted Radix Aucklandiae Radix (Comparative Example 7). From the above MIC results, it can be seen that the combination of the three medicinal materials can obtain the best in vitro antibacterial and acne-removing effect, and when Coptidis Rhizoma and Magnolia Bark were extracted using subcritical technology and Radix Aucklandiae Radix was extracted using a low eutectic solvent, excellent in vitro antibacterial and acne-removing effect can be obtained.

[0172] 3. Cell safety

[0173] Test method: HaCaT cells are a human immortalized epidermal cell line. The cytotoxicity of HaCaT cells can be used as a reference for skin safety. Normal cells have vigorous metabolism, and the succinate dehydrogenase in their mitochondria can reduce tetrazolium salts into colored crystalline substances that are deposited around the cells. This change can be read by an enzyme reader as an OD (optical density) value. By comparing it with the OD value of the blank control group, the relative growth of the cells can be known (the OD value is proportional to the cell survival rate); after treating the sample with an aqueous solution of a 0.5% concentration of the acne-removing composition, the survival rate of HaCaT cells was determined using CCK-8; a cell survival rate of more than 75% was considered "non-toxic"; the test results were referred to Figure 1 .

[0174] Combine Figure 1 It can be seen from the test results that the acne-removing compositions in Examples 1 to 6 are not cytotoxic to HaCaT cells, the compositions in Comparative Examples 5, 6, 8 to 11 are not cytotoxic to HaCaT cells, the compositions in Comparative Examples 1 to 4 are toxic to HaCaT cells, and the composition in Comparative Example 7 is weakly toxic to HaCaT cells. The above experimental results show that the combined extracts of the three raw materials of Coptis chinensis, Magnolia officinalis and Sichuan costus root provided in this application can significantly reduce the toxicity to HaCaT cells.

[0175] 4. Inhibitory effect on excessive oil secretion of sebaceous glands

[0176] The reagents used and their sources are shown in Table 3.

[0177] Table 3

[0178] Reagent name source Dimethyl sulfoxide (DMSO) Aladdin Phosphate buffered saline (PBS) Gibco Fetal bovine serum (FBS) MRC CCK8 abcam Trypsin-EDTA (0.25%), containing phenol red ThermoFisher Nile Red Solebao DMEM medium Gibco

[0179] Test method:

[0180] 1) Cell seeding: Sebaceous gland cells were seeded into 24-well plates according to a certain number and incubated in a 37°C, 5% CO2 incubator overnight;

[0181] 2) Experimental grouping: set up CTR group (solvent control group), induction group, positive control group and sample group, with 3 replicates for each group;

[0182] 3) Solution preparation: Prepare sample working solutions of different concentrations according to Table 4 below:

[0183] Table 4

[0184]

[0185] 4) Sampling: According to the experimental groups, when the cell plating rate in the 24-well plate reaches 50%, group sampling is carried out, with 3 replicate wells in each group.

[0186] 5) Nile red staining: rinsing, fixing, staining, and decolorization;

[0187] 6) Cell morphology observation: After the culture is completed, the staining of cells in each group is observed under an inverted fluorescence microscope and photographed. Figure 2 、 Figure 3 .

[0188] Combine Figure 2 and Figure 3 The results of the oil secretion inhibition test on sebaceous gland cells show that the composition provided in the present application has a strong oil secretion inhibition effect. When combined in an appropriate proportion, it has a very strong ability to inhibit oil secretion.

[0189] It can be seen from the fluorescence image of sebaceous gland cells that linoleic acid can promote the sebaceous gland cells to secrete more oil after stimulating the sebaceous gland cells, while the positive control isotretinoin and the 0.25% concentration of the anti-acne composition (Example 1) can effectively inhibit the oil secretion of sebaceous gland cells stimulated by linoleic acid after addition.

[0190] 5. Clinical acne treatment efficacy

[0191] 1) Subject selection

[0192] 1.1 The inclusion criteria for the subjects are as follows:

[0193] Healthy men or women aged between 18 and 40 years old;

[0194] People with acne on their face;

[0195] The facial skin is free of spots, moles, birthmarks, and other phenomena that may affect the test;

[0196] The subjects do not have severe chronic wasting diseases (such as asthma, diabetes, etc.);

[0197] Those who have no allergic diseases, no history of allergies to cosmetics and other external preparations (products);

[0198] Comply with the trial requirements, voluntarily participate in the trial and sign the written informed consent;

[0199] Those who have not participated in other clinical trials in the past month;

[0200] No acne-removing products or treatments (such as medication, injections, laser, or other cosmetic procedures) have been used in the past month.

[0201] 1.2 Subject exclusion criteria are as follows:

[0202] Women who are planning to become pregnant, are pregnant, breastfeeding, or within six months after giving birth;

[0203] People with severe systemic diseases, immunodeficiency or autoimmune diseases;

[0204] Those who have used antihistamines in the past week or immunosuppressants in the past month;

[0205] Those who have used any anti-inflammatory drugs in the test area in the past two months;

[0206] The tested site has participated in other clinical trials in the past three months;

[0207] People with pustules, nodules, and cystic acne on the face;

[0208] People who are receiving dermatological treatment;

[0209] Other patients who are deemed unsuitable for trial participation by clinical assessment.

[0210] 1.3 Other notes for subjects:

[0211] During the test, the test parts should use the test products provided by the test organization. Any other products with the same performance or that may affect the test results should not be used.

[0212] During the test, it is forbidden to take any preparations that may affect or potentially affect the test results (such as anti-inflammatory drugs, injections, etc.). If it is necessary to take any preparations, the test institution should be informed in advance.

[0213] During the test, the subjects maintained a regular lifestyle and paid attention to monitoring their diet:

[0214] It is prohibited to perform cosmetic procedures or treatments such as laser spot removal and skin removal that may affect the test results during the test period.

[0215] 1.4 Subject Exit Criteria:

[0216] During the test, the subject experiences adverse reactions, is lost to follow-up without reason, or violates the test protocol (such as using other methods to verify the test results).

[0217] In the case of influential cosmetics or drugs or other special reasons, after evaluation and confirmation by a dermatologist, if the subject is no longer suitable to continue the test, the subject will be asked to withdraw.

[0218] 2) Test site

[0219] There are acne-prone areas on the face.

[0220] 3) Inspection instruments

[0221] The instrument types and models are detailed in Table 5.

[0222] Table 5

[0223] instrument model source Transepidermal water loss test probe Tewameter TM300 Courage-Khazaka, Germany Skin tester VISIA 7 Canfield, United States 3D image acquisition system Antera 3D Miravex, Ireland Skin oil test probe Sebumeter SM815 Courage-Khazaka, Germany

[0224] 4) Inspection environment

[0225] Temperature 21℃±1℃

[0226] Humidity 50% ± 10% RH

[0227] 5) Inspection process

[0228] 5.1 Subject Screening

[0229] Recruit subjects as required and have them sign informed consent forms; ask subjects relevant questions based on the inclusion and exclusion criteria, and record them together with the test screening results.

[0230] 5.2 Preparation before testing

[0231] No products can be applied to the test area on the day of the visit. The subjects use a facial cleansing product provided by the laboratory that has no effect on their faces. They sit in a constant temperature and humidity room for 30 minutes. During this period, they cannot drink hot water or eat hot food, and the test area cannot be exposed to water or touched.

[0232] 5.3 Initial value test

[0233] All subjects were tested using a before-after control design. After sitting quietly in a constant temperature and humidity room for 30 minutes, they were tested using relevant instruments. A unique code was compiled and recorded, which was recorded as the initial value "D O ”.

[0234] 5.4 Follow-up test

[0235] All subjects returned for follow-up visits on the 1st, 3rd, and 7th days. No products were applied to the test areas on the days of the visits. Subjects cleansed their faces with a facial cleanser provided by the laboratory that had no efficacy. After sitting in a constant temperature and humidity chamber for 30 minutes, they were tested using a facial image collector and a skin color tester. Unique codes were compiled and recorded as test values ​​"D1", "D3", and "D7".

[0236] 5.5 Management of adverse reactions

[0237] If subjects experience adverse skin reactions while using the test samples, they should immediately stop using them, take appropriate medical protective measures, and record the adverse reaction events;

[0238] 5.6 Data Analysis

[0239] Perform statistics on the measured values ​​of each test area, including quantity, mean, standard deviation, etc. Calculate the difference between the baseline value DO and D1, D3, and D7 of each test area.

[0240] The smear sample is a gel, the composition of which is as shown in Table 6:

[0241] Table 6

[0242]

[0243] The results of the redness removal a value test refer to Figure 4 , acne volume test results refer to Figure 5 , skin oil content results refer to Figure 6 , the results of transepidermal water loss refer to Figure 7 , acne removal VISA chart reference Figure 8 .

[0244] from Figure 4 The results of the a value change rate showed that both the control and sample groups were able to reduce redness in the skin around acne, which is consistent with the repair and regulation function of human skin. The redness reduction effects of Examples 1 to 6 were significantly superior to those of the comparative example and control group. The examples reduced the redness of the skin around acne by more than 13% on day 3 and by more than 25% on day 7. The comparative example reduced the redness of the skin around acne by 6% to 12% on day 3 and by 11% to 23% on day 7.

[0245] from Figure 5 The acne volume change rate results showed that both the control and sample groups were able to remove acne, consistent with the human skin's inherent repair and regulatory functions. The acne removal effects of Examples 1 to 6 were significantly superior to those of the comparative example and control group. The examples reduced acne volume by 30% to 45% on day 3 and by 45% to 55% on day 7. The comparative example reduced acne volume by 4% to 25% on day 3 and by 8% to 40% on day 7.

[0246] from Figure 6 From the results of the change rate of skin oil content, the acne-removing effects of Examples 1 to 6 are significantly better than those of the comparative example and the control group. The examples can reduce the skin oil content by 16% to 19% on the first day, by 25% to 30% on the third day, and by 37% to 40% on the seventh day. The comparative example can reduce the skin oil content by 4% to 14% on the first day, by 14% to 23% on the third day, and by 13% to 32% on the seventh day.

[0247] from Figure 7The results of the change rate of transepidermal water loss (TEWL) showed that both the control and sample groups were able to reduce TEWL over time, a result consistent with the repair and regulation function of human skin. Based on the change rate results, Examples 1 to 6 showed significantly better acne-removing effects than the comparative example and the control group. The examples reduced TEWL in the skin surrounding acne by 5% to 8% on day 1, by more than 22% to 23% on day 3, and by more than 29% to 34% on day 7. The comparative example reduced TEWL in the skin surrounding acne by 1% to 5% on day 1, by 9% to 21% on day 3, and by 15% to 28% on day 7.

[0248] from Figure 8 According to the results of the VISA facial collection of acne changes, the volume of the acne slowly decreases over time, which is consistent with the repair and regulation function of human skin itself. After applying the gel in Comparative Example 10, on the 7th day, it can be seen that the volume of the acne is slightly reduced, and the acne protrusion (acne height) is slightly reduced. However, the VISA image still shows that the color of the acne center is still darker, indicating that the acne is still in a raised state. After applying the gel in the sample group (Example 1), on the 3rd day, it can be seen that the acne volume is significantly reduced, and the acne protrusion (acne height) is significantly reduced. On the 7th day, the VISA image shows that the center of the acne has completely lost its dark color, that is, the acne protrusion has disappeared. The above VISA images show that Example 1 has a better acne-removing effect than Comparative Example 10.

[0249] Although the present application has been described with reference to preferred embodiments, various modifications may be made thereto and components may be substituted with equivalents without departing from the scope of the present application. In particular, the various technical features described in the various embodiments may be combined in any manner as long as there are no structural conflicts. The present application is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions within the scope of the claims.

Claims

1. A method for preparing an anti-acne composition, characterized in that: The steps include: The coptis chinensis and magnolia bark were mixed and crushed, and the mixture was put into a subcritical extraction tank, and deionized water was added for high temperature and high pressure extraction, and the first extract was obtained by filtration. The mass of the coptis chinensis and magnolia bark was 7.5 kg each, and the mass of the deionized water was 100 kg; Cooling the first extract to crystallize and then drying to obtain a first extract product; Crush the costus root, add an organic base and deionized water to perform low-temperature extraction, and filter to obtain a second extract after extraction, wherein the organic base includes betaine or L-carnitine, the mass of the costus root is 7.5 kg, the mass of the deionized water is 100 kg, and the mass of the organic base is 0.75 kg; subjecting the second extract to resin adsorption and then desorption with a polyol aqueous solution to obtain a desorbed liquid; The desorption liquid is mixed with the first extraction product to obtain the anti-acne composition.

2. The preparation method according to claim 1, characterized in that The extraction temperature of the high temperature and high pressure extraction is 105° C. to 120° C., and the extraction pressure is 1.3 to 1.5 standard atmospheric pressures.

3. The preparation method according to claim 1, characterized in that The extraction temperature of the low-temperature extraction is 40°C to 55°C.

4. The preparation method according to claim 1, characterized in that The resin used for the resin adsorption includes one or both of polyamide resin and snake cage resin; and / or The polyol includes one or more of glycerol, 1,3-propylene glycol, 1,3-butylene glycol, 1,2-propylene glycol, dipropylene glycol and methylpropylene glycol; and / or The mass concentration of the polyol aqueous solution is 55% to 65%; and / or The mass ratio of the Sichuan costus root to the polyol aqueous solution is 1:(11.76~16.66).

5. Use of the anti-acne composition obtained by the preparation method according to any one of claims 1 to 4 in the preparation of anti-acne cosmetics.

6. A cosmetic, characterized in that: The invention comprises an anti-acne composition obtained by the preparation method according to any one of claims 1 to 4.

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