A drosophila circulative quantitative traditional Chinese medicine extract administration device and an experimental method thereof

Abstract

The application provides a Drosophila circulative quantitative traditional Chinese medicine extract administration device and an experimental method thereof, and belongs to the technical field of animal experiment devices. The device solves the problems of being unable to quantify, the Drosophila being prone to death and waste. The device comprises a feeder groove, a storage device and a supporting device, the feeder groove, the storage device and the supporting device are sequentially connected from top to bottom, a culture medium is placed in the feeder groove, a plurality of small holes are arranged on the feeder groove, and cotton is placed in the storage device. The Drosophila eats the culture medium through the plurality of small holes around the feeder groove, the thick culture medium is prevented from sticking to the Drosophila, the accidental death amount of the Drosophila is reduced, the Drosophila can quantitatively eat the culture medium, the Drosophila can be recycled, the device has the advantages of being green and environmentally-friendly, being easy to operate, being easy to control and observe, and improving experimental efficiency.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal experiment devices, and particularly relates to a fruit fly recyclable quantitative traditional Chinese medicine extract administration device and an experimental method thereof. BACKGROUND

[0002] Based on the excellent characteristics of fast reproduction, clear genetic background, high homologous genes with humans, and easy feeding, fruit flies have become one of the commonly selected model organisms for traditional Chinese medicine pharmacological research, and play an important role in the fields of traditional Chinese medicine anti-aging, anti-tumor, and drug screening.

[0003] In the use of fruit flies to study the pharmacology of traditional Chinese medicine extracts and traditional Chinese medicine drug screening, due to the diversity of the physical and chemical properties of traditional Chinese medicine extracts, such as odor, solubility difference, color diversity, and different acid-base properties, in the process of fruit fly administration, the common operation is to mix traditional Chinese medicine extracts with fruit fly culture feed (such as sucrose, corn flour, yeast, etc.) to configure a solid culture medium containing traditional Chinese medicine extracts for fruit flies to eat.

[0004] The existing method is to mix traditional Chinese medicine extracts with fruit fly feed, and then put them into fruit fly culture tubes or fruit fly culture bottles. However, this method has the following problems in research: first, in the study of fruit fly survival analysis, it is impossible to quantify whether the fruit flies have eaten the culture medium, and to determine whether the fruit flies have died due to eating the medicated culture or have starved to death; second, there is a situation that the medicated culture medium is sticky, which can stick fruit flies and cause fruit fly death; third, the insufficient use of medicated culture medium causes waste, and the medicated culture medium attached to the bottom and the inner wall of the fruit fly tube or bottle causes fruit flies to only eat the medicated culture medium exposed on the surface; fourth, the one-time use of fruit fly tubes or bottles causes waste and is not environmentally friendly, and the repeated use requires a lot of time for cleaning, which greatly affects the experimental efficiency. SUMMARY

[0005] Therefore, in order to solve the technical problems mentioned in the background, the present application provides a fruit fly recyclable quantitative traditional Chinese medicine extract administration device and an experimental method thereof, which has the advantages of quantifying the fruit fly culture medium added with traditional Chinese medicine extracts, reducing accidental death of fruit flies, increasing the area of fruit fly eating medicated culture medium, being green and environmentally friendly, being easy to operate, control and observe, and improving experimental efficiency.

[0006] To achieve the above purpose, the present application adopts the following technical solution: a fruit fly recyclable quantitative traditional Chinese medicine extract administration device, comprising a dosing device groove, a storage device and a supporting device, the dosing device groove, the storage device and the supporting device are connected in sequence from top to bottom, the culture medium is placed in the dosing device groove, a plurality of small holes are arranged on the dosing device groove, and cotton is placed in the storage device.

[0007] Further, the medicine dispenser groove is made of transparent polypropylene material, the upper part of the medicine dispenser groove is a cuboid, the middle part is a four-rhombic pyramid, and the bottom part is a cylinder.

[0008] Further, the upper part of the medicine dispenser groove is more than 80% of the bottom surface of each hole.

[0009] Further, the upper and lower end faces of the cylinder are provided with a plurality of bottom holes of the medicine dispenser groove.

[0010] Further, the top of the medicine dispenser groove is provided with a top switch.

[0011] Further, the storage device, the medicine dispenser groove and the support device are connected by threads.

[0012] Further, the storage device is a cylinder in shape.

[0013] Further, the support device comprises a connecting part and a plurality of support feet, and the connecting part is provided with a plurality of support feet.

[0014] Further, the support feet are telescopic and provided with a suction cup at the bottom.

[0015] An experimental method of a fruit fly circulatory quantitative traditional Chinese medicine extract administration device, specifically comprising the following steps:

[0016] (1) Prepare the culture medium, which is a traditional Chinese medicine extract culture medium, then place the fruit fly circulatory quantitative traditional Chinese medicine extract administration device and open the top switch 1, add the prepared culture medium to the medicine dispenser groove, close the top switch and cool it down, and save it for later use;

[0017] (2) Weigh the total mass of the fruit fly circulatory quantitative traditional Chinese medicine extract administration device with culture medium and record M1, place the fruit fly tube in it, and put the sorted male and female fruit flies into the fruit fly tube in advance, and use carbon dioxide gas to anesthetize them, record the number of dead fruit flies every day, anesthetize the fruit flies after two days, take them out and weigh the total mass of the fruit fly circulatory quantitative traditional Chinese medicine extract administration device again and record M2, and calculate the formula for the amount of culture medium consumed by fruit flies after two days: Δ M=M2-M1;

[0018] (3) Statistically analyze the results and analyze the amount of culture medium consumed by fruit flies over time, and statistically analyze the number of dead males and females and analyze the effect of culture medium on the survival life of fruit flies.

[0019] Compared with the prior art, the fruit fly circulatory quantitative traditional Chinese medicine extract administration device and the experimental method thereof have the following advantages:

[0020] (1) The fruit fly in the present application eats the culture medium through the small holes around the groove of the dosing device, which prevents the culture medium from sticking to the fruit fly and reduces the accidental death of the fruit fly.

[0021] (2) The whole device is taken out again after each time period, and the quantitative calculation before and after is carried out, so that the fruit fly culture medium with added traditional Chinese medicine extract can be quantified.

[0022] (3) In the present application, the medicated culture medium is elevated from the previously exposed surface to five surfaces, increasing the dosing area and greatly reducing the waste of medicated culture medium.

[0023] (4) In terms of operation and raw materials, the present application is easy to operate and observe, and can greatly improve the experimental efficiency; the device raw materials have abundant sources and are easy to make.

[0024] (5) The present application is green, easy to operate, control and observe, and can improve the experimental efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0025] The accompanying drawings, which form a part of the present application, are used to provide a further understanding of the present application, and the illustrative embodiments thereof and their descriptions are used to explain the present application and do not constitute improper limitations on the present application. In the drawings:

[0026] Figure 1 The structure diagram of the fruit fly circulating quantitative traditional Chinese medicine extract dosing device described in the present application;

[0027] Figure 2 The detailed diagram of the dosing device groove of the fruit fly circulating quantitative traditional Chinese medicine extract dosing device described in the present application;

[0028] Figure 3 The detailed diagram of the storage device and connecting device of the fruit fly circulating quantitative traditional Chinese medicine extract dosing device described in the present application;

[0029] Figure 4 The detailed diagram of the support device of the fruit fly circulating quantitative traditional Chinese medicine extract dosing device described in the present application.

[0030] In the figure: 1 - top switch; 2 - small hole; 3 - connecting device; 4 - support device; 5 - dosing device groove bottom small hole; 6 - dosing device groove bottom convex thread; 7 - concave thread; 8 - storage device; 9 - support device convex thread; 10 - movable rod; 11 - support foot. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict, and the described embodiments are only some of the embodiments of the present application, but not all the embodiments.

[0032] Referring to Figures 1-4 The present embodiment is described as follows. A fruit fly circulatory quantitative traditional Chinese medicine extract administration device is composed of three parts, which are sequentially arranged from top to bottom. The first part is a transparent polypropylene administration groove without odor. The upper part of the administration groove is a cuboid, the middle part is a four-rhombic cone, the bottom part is a cylinder, and the top part is provided with a top switch 1. Five sides (with a thickness of 1 mm) are respectively provided with small holes (each side is provided with small holes with a proportion of at least 80% or more). The cylinder-shaped tube connected to the bottom part is provided with a convex thread design, and the upper and lower surfaces are provided with small holes 5 at the bottom of the administration groove. The second part is a storage device, which is made of the same material as the first part and is composed of a cylinder and a cone. The inside of the cylinder tube is provided with a thread design, and the inside of the cone is smooth. The internal space is used for placing cotton. The third part is a support device, which is mainly made of metal. The upper part is a cylinder with a thread design. The lower part is composed of three support legs 11. Each support leg 11 can be telescopic, and each support leg 11 is provided with a plastic suction cup at the bottom.

[0033] The working principle of the fruit fly circulatory quantitative traditional Chinese medicine extract administration device is as follows.

[0034] The fruit fly circulatory quantitative traditional Chinese medicine extract administration device with added traditional Chinese medicine extract is installed in a fruit fly tube after the total mass is weighed. The fruit flies eat the culture medium through the small holes around the administration groove, so as to prevent the fruit flies from being stuck by the viscous culture medium. Under the action of gravity, part of the excess water in the culture medium flows out and is collected in the cotton of the storage device 3, so as to reduce the viscosity of the culture medium. After each period of time, the whole device is taken out and weighed again, the quantitative calculation before and after is performed, and the administration groove with added traditional Chinese medicine extract is replaced (only the administration groove with added medicine needs to be replaced, and the remaining device can not be replaced).

[0035] The present embodiment provides an experimental method using the fruit fly circulatory quantitative traditional Chinese medicine extract administration device. The device is especially suitable for pharmacological experiments such as fruit fly survival analysis, and specifically includes the following steps.

[0036] (1) Taking the survival analysis experiment as an example, the preparation of the culture medium for the herbal extract of Drosophila involved weighing 52.5g corn flour, 27.5g sucrose, 20g yeast, and 8g agar, stirring evenly, and dissolving in 500ml pure water. The solution was heated to boiling on an induction cooker for 5 minutes. After cooling to 60℃, 0.25ml propionic acid was added and stirred evenly. The solution was then placed in a 60℃ water bath, and the required amount of herbal extract was added and mixed evenly. The recyclable quantitative herbal extract drug delivery device for Drosophila was set up and turned on. The prepared culture medium was added to the drug delivery device tank, the switch was turned off, and the solution was allowed to cool. It was then stored at 4℃ for later use.

[0037] (2) When conducting the experiment, weigh the total mass of the fruit fly recyclable quantitative Chinese medicine extract administration device containing the Chinese medicine extract culture medium and record it as M1. Take a 50mm×120mm fruit fly tube and place it in the device. Beforehand, select male and female fruit flies for the experiment, anesthetize them with carbon dioxide gas, and put them into the fruit fly tubes. Record the number of dead fruit flies every day. After two days, anesthetize the fruit flies, take them out, weigh the total mass of the fruit fly recyclable quantitative Chinese medicine extract administration device again, and record it as M2. The formula for calculating the quantitative amount of culture medium consumed by the fruit flies after two days is: Δ M = M2 - M1. Replace the cotton in the new drug delivery container and the collection container 3 (only the used drug delivery container needs to be cleaned and the cotton replaced; the fruit fly tube and other devices can be reused multiple times).

[0038] (3) Finally, the results were statistically analyzed and the amount of food consumed by fruit flies over time was analyzed using the Chinese herbal extract culture medium. The number of male and female flies that died was also statistically analyzed and the effect of the Chinese herbal extract culture medium on the lifespan of fruit flies was analyzed.

[0039] The embodiments of the present invention disclosed above are merely illustrative of the invention. These embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention.

Claims

1. An experimental method using a fruit fly recyclable quantitative traditional Chinese medicine extract delivery device, characterized in that: Specifically, the following steps are included: The fruit fly recyclable quantitative Chinese medicine extract administration device includes an administration device slot, a receiver (3) and a support device (4). The administration device slot, receiver (3) and support device (4) are connected in sequence from top to bottom. The administration device slot contains a culture medium and has several small holes (2). The receiver (3) contains cotton. The specific experimental method includes the following steps: (1) Prepare a culture medium, which is a traditional Chinese medicine extract culture medium. Then, place the fruit fly recyclable quantitative traditional Chinese medicine extract drug delivery device and turn on the top switch (1). Add the prepared culture medium to the drug delivery tank, turn off the top switch and wait for the culture medium to cool down, and store it for later use. (2) Weigh the total mass of the fruit fly recyclable quantitative Chinese medicine extract administration device with culture medium and record it as M1. Take a fruit fly tube and put the device into it. In advance, select the male and female fruit flies for the experiment, anesthetize them with carbon dioxide gas and put them into the fruit fly tube. Record the number of dead fruit flies every day. After two days, anesthetize the fruit flies, take them out and weigh the total mass of the fruit fly recyclable quantitative Chinese medicine extract administration device again and record it as M2. The formula for calculating the quantitative amount of culture medium consumed by the fruit flies for two days is: ∆M=M2-M1; (3) Statistical analysis of quantitative results and analysis of the amount of food consumed by fruit flies over time, statistical analysis of the number of male and female deaths and analysis of the effect of culture medium on the lifespan of fruit flies; The drug delivery device slot is made of transparent polypropylene. The upper part of the drug delivery device slot is rectangular, the middle part is a square pyramid, and the bottom is cylindrical. The upper part of the drug delivery device slot, except for the bottom surface, has a small hole (2) accounting for >80% of each side; The upper and lower end faces of the cylinder are provided with several small holes at the bottom of the drug delivery slot (5). A top switch (1) is provided at the top of the drug delivery device slot.

2. The experimental method for using the fruit fly recyclable quantitative traditional Chinese medicine extract delivery device according to claim 1, characterized in that: The receiver (3) is threadedly connected to the applicator slot and the support device (4).

3. The experimental method for using the fruit fly recyclable quantitative traditional Chinese medicine extract delivery device according to claim 1, characterized in that: The storage container (3) is cylindrical at both the top and bottom.

4. The experimental method for using the fruit fly recyclable quantitative traditional Chinese medicine extract delivery device according to claim 1, characterized in that: The support device (4) includes a connecting part and several support feet (11), with several support feet (11) installed below the connecting part.

5. The experimental method for using the fruit fly recyclable quantitative traditional Chinese medicine extract delivery device according to claim 4, characterized in that: The support foot (11) is retractable and has a suction cup at the bottom.

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