A method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships

Abstract

The invention discloses a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, comprising the steps of: 2‑ Separate water inlet: intermittently introduce S into the reaction system inoculated with anaerobic activated sludge. 2‑ The first type of seawater transformation, water inlet and stirring; S 2‑ The individual water inlet settings are five gradients S 2‑ concentration, each gradient corresponds to a reaction stage. When the NO3 ‑ , S 2‑ When the removal rate of total organic carbon reaches a stable level, the influent S 2‑ The concentration is adjusted to the next concentration gradient; S 2‑ While the water is being fed separately, NO3 is added to the seawater by continuous water inlet. ‑ and organic matter in the second stage of seawater transformation; activated carbon particles are added in the second stage; in the fifth stage and S 2‑ When the removal rate reaches more than 80%, the reaction stops. 2‑ concentration, gradually acclimate the autotrophic desulfurization and denitrification functional bacteria to S 2‑ The degradation capacity of sulfate-reducing bacteria on S 2‑ Toxicity resistance, increased inhibition and elimination of heterotrophic denitrification, high concentration of S 2‑ Under the same conditions, the comprehensive and rapid transformation from heterotrophic to sulfur autotrophic denitrification can be achieved within 30 days. At the same time, combined with stirring and adding activated carbon, S 2‑ and NO3 ‑ The removal rates reached high levels of 84.24% and 97.46% respectively, achieving efficient removal of multiple pollutants.

Description

Technical Field

[0001] The invention relates to the technical field of ship sewage desulfurization and denitrification, and in particular to a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in ship high-salinity wastewater. Background Art

[0002] Shipping is one of the main modes of international trade, with the advantages of high efficiency, economy, convenience and large transportation capacity. However, during ship operation, the exhaust gas emitted by diesel engines contains sulfur oxides (SO x ) and nitrogen oxides (NO x ), which brings pollution risks to the atmosphere. At present, ship flue gas treatment mostly adopts open wet scrubbing method, which uses seawater to absorb SO in flue gas. x and NO x , converting it into SO4 2- 、SO3 2- and NO3 - Plasma remains in the waste liquid. At the same time, ships also produce a large amount of domestic sewage during navigation, which contains high-concentration organic matter. If the above high-salt wastewater is directly discharged into the sea, it will seriously damage the marine environment.

[0003] In recent years, biological methods based on microbial metabolic capacity have been applied to the simultaneous desulfurization and denitrification of high-salinity wastewater. How to improve the operating efficiency of this process has gradually become the focus of application attention. In an anaerobic environment, sulfate reduction is coupled with autotrophic desulfurization and denitrification reactions. Sulfate-reducing bacteria use organic matter as electron donors to convert SO4 2- Restore to S 2- , followed by autotrophic desulfurization and denitrification with S 2- Reduction of NO3 as electron donor - , S 2- Oxidized to S 0 Recycle, NO3 - is reduced to N2. However, the wastewater contains NO3 - Organic matter is a beneficial substrate for heterotrophic denitrifying microorganisms. Organic matter can be converted into NO3 through heterotrophic denitrification. - Therefore, in the integrated reactor, heterotrophic denitrifying microorganisms will compete with sulfate-reducing functional bacteria for organic matter and with autotrophic desulfurization and denitrification bacteria for substrate NO3 - , which reduces the operating efficiency of the simultaneous desulfurization and denitrification process and hinders the widespread application of integrated reactors.

[0004] Therefore, in order to increase the intensity of autotrophic desulfurization and denitrification reactions and the relative abundance of autotrophic denitrifying bacteria, it is necessary to inhibit and prevent the heterotrophic denitrification reaction dominated by heterotrophic denitrifying microorganisms. 2-It will have a shocking toxic effect on the overall microbial flora, making it difficult to achieve a stable and rapid transformation from heterotrophic denitrification to sulfur autotrophic denitrification. Therefore, it is necessary to invent a reasonable step-by-step method to promote the succession of functional microorganisms to complete the transformation of the core reaction system. Summary of the invention

[0005] The present invention provides a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, so as to solve the above problems.

[0006] In order to achieve the above object, the technical solution of the present invention is:

[0007] A method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, comprising the steps of:

[0008] S 2- Separate water inlet: intermittent water inlet into the reactor inoculated with anaerobic activated sludge, the water inlet is S added to the seawater 2- The first type of transformed seawater is 0.2-0.6L of water per hour, with water added once every 10 minutes, each time for 5-10 minutes, and stirring during the water addition; S 2- The individual water inlet settings are five gradients S 2- concentration, and each gradient corresponds to a reaction stage. When the NO3 - Removal rate, S 2- When the removal rate and total organic carbon removal rate reach a stable level, the influent S 2- The concentration is adjusted to the next concentration gradient for the next reaction stage; continuous stirring allows sufficient contact between the activated sludge and the activated carbon particles, and sufficient mass transfer between the influent substrate and the microorganisms, thereby promoting the formation of biofilm and the degradation of pollutants. The reaction efficiency is improved under the joint action of the biofilm and the suspended activated sludge; intermittent stirring allows the activated sludge to have a reasonable sedimentation time to prevent excessive loss of functional microorganisms with the effluent;

[0009] S 2- At the same time as the water is fed separately, the reaction system in the reactor is continuously fed with water to add NO3 - and the second type of modified seawater of organic matter;

[0010] When the reaction is in the second stage, activated carbon particles are added to the reaction system to promote the attachment and growth of flagellated autotrophic desulfurization and denitrification functional bacteria. At the same time, since the density of activated carbon is greater than that of activated sludge, it can increase the density and viscosity of the overall flow system, which is beneficial to the sedimentation of activated sludge and prevents sludge expansion and excessive loss of functional bacteria.

[0011] When the reaction is in the fifth stage, and S 2- When the removal rate reaches more than 80%, the reaction stops.

[0012] Furthermore, the five gradients S 2- S between adjacent gradients of the first three gradients in concentration 2- The concentration difference is no more than 30 mgS / L. The S between the adjacent gradients of the latter two gradients is 2- The concentration difference is greater than 30 mgS / L; the concentration difference of the first three gradients is small, which can induce the proliferation of autotrophic desulfurization and denitrification bacteria under the condition of ensuring the normal metabolism of sulfate-reducing bacteria, and enhance the autotrophic desulfurization and denitrification bacteria to S 2- degradation capacity of sulfate-reducing bacteria and domestication of S 2- Adaptability to avoid subsequent S 2- The shock toxicity caused by excessive concentration destroys the sulfur degradation fundamentals of the reaction system; the substantial increase in the concentration difference of the latter two gradients can increase the proliferation driving force of autotrophic desulfurization and denitrification bacteria, triggering a rapid increase in their abundance, increasing the inhibition of heterotrophic denitrification, and substantially reducing the relative abundance of heterotrophic denitrification bacteria, thus achieving a rapid transformation from heterotrophic to sulfur autotrophic denitrification.

[0013] Furthermore, the five gradients S 2- The concentrations are 0mgS / L, 30mgS / L, 50mgS / L, 150mgS / L and 200mgS / L, which are generated by the reduction of sulfate contained in the background of seawater to produce 250mgS / L-270mgS / L 2- Add S on the basis of 2- The overall concentration is much higher than that of S removed by conventional desulfurization and denitrification processes. 2- Concentration. Such a high concentration can provide the maximum electron donor for autotrophic desulfurization and denitrification, provide beneficial substrates for inducing the rapid proliferation of autotrophic denitrifying bacteria, quickly inhibit heterotrophic denitrification reactions, reduce the abundance of heterotrophic bacteria including heterotrophic denitrifying bacteria and fermentation bacteria, and help autotrophic desulfurization and denitrification bacteria and sulfate-reducing bacteria gain an advantage in the competition with heterotrophic denitrifying bacteria and fermentation bacteria.

[0014] Furthermore, the introduced NO3 - The concentration is 100 mg / L-200 mg / L in terms of N, the concentration of organic matter is 350 mg / L-400 mg / L in terms of C, the pH value is 7.0-8.0, and it remains unchanged throughout the reaction.

[0015] Furthermore, the stirring speed is 5-15 r / min, the time is 5-15 min, and the temperature is set to 30-35°C.

[0016] Furthermore, the volume of the activated carbon particles added accounts for 5%-15% of the effective volume of the reactor, and the particle size of the activated carbon particles is 0.1-0.3 cm.

[0017] Furthermore, the NO3 in the effluent of each stage is determined - Removal rate, S 2- The removal rate and total organic carbon removal rate both reached a stable state. Specifically, the difference in removal rate measured before and after every 24 hours was no more than 5%.

[0018] Furthermore, the first type of modified seawater comprises: natural seawater 900-950 mg SO4 2- -S / L, Na2S·9H2O 0-200mgS / L;

[0019] The second type of modified seawater includes: natural seawater 900-950mg SO4 2- -S / L, peptone 380mgC / L, NaNO3150mgN / L, NaHCO3 3g / L.

[0020] Furthermore, the concentration of the inoculated anaerobic activated sludge is 5-10 gMLVSS / L, and the inoculation volume accounts for 30-35% of the effective reaction volume of the reactor, which not only maintains the microbial concentration in the reaction system, but also ensures that the activated sludge does not over-expand and be lost under stirring conditions.

[0021] The beneficial effects of the present invention are:

[0022] The present invention discloses a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, by increasing S 2- concentration, induced the proliferation of autotrophic functional bacteria, and gradually domesticated the autotrophic desulfurization and denitrification functional bacteria to S 2- The degradation capacity of sulfate-reducing bacteria on S 2- The resistance to toxicity can be enhanced, the intensity of autotrophic desulfurization and denitrification reaction can be increased, the inhibition and elimination of heterotrophic denitrification can be increased, and the stable flow of electrons released by organic matter to sulfate reduction reaction can be promoted, so that autotrophic desulfurization and denitrification replaces heterotrophic denitrification as the main reaction. 2- Under the same conditions, the rapid transformation from heterotrophic to sulfur autotrophic denitrification can be achieved within 30 days. The relative abundance of autotrophic bacteria can reach 20 times that of heterotrophic denitrifying bacteria. At the same time, combined with stirring and adding activated carbon, S 2- and NO3 - The removal rates can reach high levels of 84.24% and 97.46% respectively, achieving efficient removal of multiple pollutants, so that nitrogen and sulfur pollutants are ultimately converted into environmentally friendly N2 and S 0 It neither introduces new pollutants nor easily causes secondary pollution, ensuring the stable and efficient operation of the integrated desulfurization and denitrification process, saving ship space, and reducing the startup and stabilization time of biological wastewater treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 The relative abundance spectrum of dominant bacterial genera in the reaction system of a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships disclosed in an embodiment of the present invention;

[0025] Figure 2 It is the removal rate of various pollutants in the reaction system of a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships disclosed in an embodiment of the present invention. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The principle involved in the present invention:

[0028] In an anaerobic environment, sulfate reduction is coupled with autotrophic desulfurization and denitrification reactions. Sulfate-reducing bacteria use organic matter as electron donors to convert SO4 2- Restore to S 2- , the reaction is shown in formula (1); subsequently, autotrophic desulfurization and denitrification with S 2- Reduction of NO3 as electron donor - , S 2- Oxidized to S 0 Recycle, NO3 - is reduced to N2, as shown in equation (2); however, the wastewater contains NO3 - Organic matter is a beneficial substrate for heterotrophic denitrifying microorganisms. Organic matter can be converted into NO3 - The reduction of the heterotrophic denitrifying microorganisms provides electrons, as shown in equation (3). Therefore, in the integrated reactor, the heterotrophic denitrifying microorganisms will compete with the sulfate-reducing functional bacteria for organic matter, and will compete with the autotrophic desulfurization and denitrifying bacteria for the substrate NO3 - , which reduces the operating efficiency of the simultaneous desulfurization and denitrification process and hinders the widespread application of integrated reactors.

[0029] Org-C+SO4 2- →S 2- +CO2+H2O (1)

[0030] 5S 2- +2NO3 - +12H + →5S 0 +N2+6H2O (2)

[0031] Org-C+NO3 - +H2O→OH - +N2+CO2 (3)

[0032] Therefore, in order to improve the operating efficiency of the simultaneous desulfurization and denitrification process, it is necessary to increase the relative abundance of autotrophic denitrifying bacteria and increase the reaction intensity of autotrophic desulfurization and denitrification, inhibit the growth of heterotrophic denitrifying microorganisms and reduce the intensity of heterotrophic denitrification reactions. 2- It has toxic inhibitory effects on heterotrophic microorganisms, while S 2- It is also a beneficial substrate for autotrophic desulfurization and denitrification bacteria. Under certain control conditions, it can be quickly consumed without affecting the effluent quality of the integrated process. Therefore, in order to weaken the heterotrophic denitrification reaction on organic matter and NO3 - The competitive consumption of sulfur autotrophic bacteria is enhanced, thereby driving the occurrence of autotrophic desulfurization and denitrification, so that autotrophic desulfurization and denitrification replaces heterotrophic denitrification as the main reaction in the reaction system. The present invention proposes a method for inducing the rapid conversion of heterotrophic to sulfur autotrophic denitrification in ship high-salinity wastewater. By increasing S 2- The concentration was combined with stirring and adding activated carbon into the system to gradually acclimate the autotrophic desulfurization and denitrification functional bacteria to S 2- The degradation capacity of sulfate-reducing bacteria on S 2- The resistance of sulfur autotrophic denitrification is enhanced, the inhibition of heterotrophic denitrifying microorganisms is increased, the intensity of heterotrophic denitrification and anaerobic fermentation is weakened, and the electrons released by organic matter are promoted to flow steadily to the sulfate reduction reaction, so that autotrophic desulfurization and denitrification replace heterotrophic denitrification as the main reaction, and the comprehensive and rapid transformation from heterotrophic to sulfur autotrophic denitrification is realized, so that S 2- and NO3 - The removal rate can reach a high level, achieving efficient removal of multiple pollutants.

[0033] Example:

[0034] This embodiment provides a method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, comprising the steps of:

[0035] The anaerobic activated sludge from the secondary sedimentation tank of the municipal sewage treatment plant was inoculated into the integrated reactor, the sludge concentration was 9.87 gMLVSS / L, and the inoculation volume accounted for 30% of the effective reaction volume of the reactor; S 2- The intermittent water inlet is the first type of transformed seawater, that is, natural seawater (900mgSO4 2- -S / L) was added with Na2S·9H2O (0-200mgS / L); other substrates were continuously fed with water as the second type of modified seawater, that is, natural seawater (900mgSO4 2- -S / L) was added with peptone (380 mgC / L), NaNO3 (150 mgN / L) and NaHCO3 (3 g / L);

[0036] S 2- The water was fed intermittently, with 0.4 L of water fed per hour, once every 10 minutes, and each time for 10 minutes. Stirring was performed while feeding water, with a stirring speed of 10 r / min and a time of 10 min. The S in the reactor was gradually increased. 2- The concentrations, in terms of S, are 0 mg / L, 30 mg / L, 50 mg / L, 150 mg / L, and 200 mg / L, and correspond to the five stages of the reaction, J1, J2, J3, J4, and J5, respectively. When the NO3 in the effluent of each stage - Removal rate, S 2- When the removal rate and total organic carbon removal rate of each 24 hours before and after the removal rate difference is no more than 5%, adjust the next stage S 2- concentration, allowing the reaction to enter the next stage; adding S 2- At the same time, 10% of the effective volume of the reactor was added with activated carbon particles (i.e., the reaction was in S 2- In the second stage with a concentration of 30 mg / L), the particle size of the activated carbon particles was 0.2 cm, and other substrates were continuously fed with water. - The concentration is 150 mg / L in terms of N, the organic matter concentration is 380 mg / L in terms of C, the pH value is 7.5, and the temperature is 35±0.5℃. When the reaction is in S 2- The fifth stage with a concentration of 200 mg / L and NO3 in the effluent - Removal rate and S 2- When the removal rate reaches more than 80%, the reaction stops.

[0037] Detect NO3 in water every 24 hours - Concentration, S 2- concentration and total organic carbon concentration, according to the measured effluent NO3 - Removal rate, S 2- Removal rate and total organic carbon removal rate, judge the intensity of heterotrophic denitrification and autotrophic desulfurization and denitrification reactions and the degree of succession of heterotrophic denitrifiers and autotrophic desulfurization and denitrification bacteria;

[0038] Gradually increase S 2- The concentration makes the intensity of heterotrophic denitrification gradually weaken, and finally the heterotrophic degradation of NO3 - and organic matter decreased; while NO3 - At the same time, it acts as an electron acceptor for autotrophic desulfurization and denitrification. 2- In the presence of, it can promote the autotrophic desulfurization and denitrification. Therefore, in the process of rapid transformation from heterotrophic denitrification to autotrophic desulfurization and denitrification, NO3 - The removal of SO4 can still reach a high level; due to the presence of a large amount of SO4 2- The electrons released by organic matter can be used by sulfate reduction, which weakens the ability of heterotrophic denitrification to compete with organic matter and promotes sulfate reduction reaction to use organic matter to convert SO4 2- Restore to S 2- , enhancing the inhibition of heterotrophic denitrification and facilitating the rapid transformation of heterotrophic to sulfur autotrophic denitrification.

[0039] Gradually increase S 2- The concentration can cause a stable succession of functional bacteria. After the overall adjustment, heterotrophic denitrifiers and carbon degradation bacteria are inhibited, and the metabolic capacity of autotrophic desulfurization and denitrification bacteria is enhanced. 2- Under the condition of concentration, carbon degradation bacteria are inhibited first, and heterotrophic denitrification bacteria begin to be inhibited when the concentration reaches medium and high. 2- As the concentration increases, the functional bacterial genus undergoes succession, with S 2- The abundance of autotrophic desulfurization and denitrification bacteria using chlorinated parasites as substrates increased significantly, and the dominant bacterial genus evolved into autotrophic desulfurization and denitrification bacteria.

[0040] (1) Bacterial genus abundance:

[0041] After each stage of reaction, activated sludge samples were collected at specific locations inside the reactor, and the microorganisms in the reaction system were identified by DNA extraction, PCR amplification, purification, fluorescence quantification and high-throughput sequencing (all existing methods). The main dominant bacterial genera are shown in the following table. Figure 1 As shown, with S 2- As the concentration increased, the carbon-degrading bacteria Marinifilum and Psychromonas were first inhibited; 2- When the concentration reached a high concentration of 150 mg / L, the inhibition of the heterotrophic denitrifying bacteria Halomonas was significantly enhanced, and the relative abundance was greatly reduced; the autotrophic desulfurizing denitrifying bacteria Sulfurovum 2- Under sufficient conditions, the relative abundance reaches 20 times that of heterotrophic denitrifiers, becoming the absolute dominant genus;

[0042] (2) Removal of various pollutants:

[0043] The removal effect of each pollutant is as follows Figure 2 As shown, in S 2- In the later stage of concentration adjustment, the reaction is in S 2- In the fifth stage, the concentration of NO3 in the effluent is 200 mg / L. - Removal rate, S 2- The removal rates of sulfur dioxide and total organic carbon reached 97.46%, 84.24% and 71.11% respectively, indicating that the main reaction in the system was autotrophic desulfurization and denitrification, and various pollutants were removed synergistically. 2- Under the optimal conditions, the comprehensive and rapid transformation from heterotrophic to sulfur autotrophic denitrification can be achieved within 30 days.

[0044] In summary, this method gradually increases S 2- concentration, induced the proliferation of autotrophic functional bacteria, and gradually domesticated the autotrophic desulfurization and denitrification functional bacteria to S 2- The degradation capacity of sulfate-reducing bacteria on S 2- The resistance of the ship's high-salinity wastewater is enhanced, the intensity of the autotrophic desulfurization and denitrification reaction is enhanced, and the heterotrophic denitrification reaction in the simultaneous desulfurization and denitrification process of the ship's high-salinity wastewater is inhibited, and its resistance to organic matter and NO3 is weakened. - The competitive consumption of seawater makes autotrophic denitrification gradually replace heterotrophic denitrification as the main reaction; at the same time, it can also improve the inhibitory effect on anaerobic fermentation bacteria, prevent the electrons released by organic matter from being lost through fermentation and other processes, and is conducive to the survival of autotrophic denitrification functional bacteria; the salinity of seawater and the sulfate it contains promote the growth of sulfate-reducing bacteria and the occurrence of sulfate reduction reaction, and S will also be produced inside the system. 2- , further promoting the inhibition of heterotrophic reactions and promoting the comprehensive and rapid transformation from heterotrophic to autotrophic desulfurization and denitrification. 2- concentration, avoid S 2- The shock toxicity caused by the one-time increase in concentration destroys the sulfur degradation fundamentals of the reaction system, and can induce the proliferation of autotrophic desulfurization and denitrification bacteria under the condition of ensuring the normal metabolism of sulfate-reducing bacteria, increase the inhibition of heterotrophic denitrification, greatly reduce the relative abundance of heterotrophic denitrification bacteria, and achieve a comprehensive and rapid transformation from heterotrophic to sulfur autotrophic denitrification, so that S 2- and NO3 - The removal rates can reach a high level of 84.24% and 97.46% respectively, achieving efficient removal of multiple pollutants, so that nitrogen and sulfur pollutants are finally converted into N2 and S 0 It neither introduces new pollutants nor easily causes secondary pollution, ensuring the stable and efficient operation of the integrated desulfurization and denitrification process, saving ship space and reducing the startup and stabilization time of the reaction system.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships, characterized in that: Includes steps: S 2- Separate water inlet: intermittent water inlet into the reactor inoculated with anaerobic activated sludge, the water inlet is S added to the seawater 2- The first type of transformed seawater is 0.2-0.6L of water per hour, with water added once every 10 minutes, each time for 5-10 minutes, and stirring during the water addition; S 2- The individual water inlet settings are five gradients S 2- concentration, and each gradient corresponds to a reaction stage. When the NO3 - Removal rate, S 2- When the removal rate and total organic carbon removal rate reach a stable level, the influent S 2- The concentration is adjusted to the next concentration gradient to proceed to the next reaction stage; S 2- At the same time as the water is fed separately, the reaction system in the reactor is continuously fed with water to add NO3 - and the second type of modified seawater of organic matter; When the reaction is in the second stage, activated carbon particles are added to the reaction system; When the reaction is in the fifth stage, and S 2- When the removal rate reaches more than 80%, the reaction stops; The five gradients S 2- The concentrations are 0 mgS / L, 30 mgS / L, 50 mgS / L, 150 mgS / L and 200 mgS / L respectively.

2. A method for rapidly converting heterotrophic to sulfur autotrophic denitrification in ship high-salinity wastewater according to claim 1, characterized in that: The second type of transformation of NO3 in seawater - The concentration is 100 mg / L-200 mg / L in terms of N, the concentration of organic matter is 350 mg / L-400 mg / L in terms of C, the pH value is 7.0-8.0, and it remains unchanged throughout the reaction.

3. The method for rapidly converting heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships according to claim 1, characterized in that: The stirring speed is 5-15 r / min, the time is 5-15 minutes, and the temperature is set to 30-35°C.

4. The method for rapidly converting heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships according to claim 1, characterized in that: The added volume of the activated carbon particles accounts for 5%-15% of the effective volume of the reactor, and the particle size of the activated carbon particles is 0.1-0.3 cm.

5. The method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships according to claim 1, characterized in that: Determine the NO3 in the effluent at each stage - Removal rate, S 2- The removal rate and total organic carbon removal rate reached a stable state. The specific method is: the difference in removal rate measured before and after every 24 hours is no more than 5%.

6. The method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in high-salinity wastewater from ships according to claim 1, characterized in that: The first type of modified seawater includes: natural seawater 900-950mg SO4 2- -S / L, Na2S·9H2O 0-200mgS / L; The second type of modified seawater includes: natural seawater 900-950mg SO4 2- -S / L, peptone 380mgC / L, NaNO3150mgN / L, NaHCO33g / L.

7. The method for inducing rapid conversion of heterotrophic to sulfur autotrophic denitrification in ship high-salinity wastewater according to claim 1, characterized in that: The concentration of the inoculated anaerobic activated sludge is 5-10 gMLVSS / L, and the inoculation volume accounts for 30%-35% of the effective reaction volume of the reactor.

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